Nitrogen, an essential element for plant growth and food production, presents significant challenges in agriculture due to the environmental consequences of synthetic nitrogen fertilizers. This review explores the potential of nitrogen-fixing cyanobacteria as a sustainable alternative for agricultural nitrogen fertilization. The molecular mechanisms underlying nitrogen fixation in cyanobacteria, including key genes such as nif and related biochemical pathways, are examined in detail. Biotechnological approaches for utilizing nitrogen-fixing cyanobacteria as biofertilizers are discussed, alongside strategies for genetic engineering to improve nitrogen fixation efficiency. The review further evaluates the impact of cyanobacteria on soil health and environmental sustainability, emphasizing their role in mitigating the detrimental effects of synthetic fertilizers. While promising, challenges such as oxygen sensitivity during nitrogen fixation and competition with native microorganisms are critically analyzed. Finally, future directions are proposed, including advancements in synthetic biology, integration with conventional agricultural practices, and scalable implementation strategies. This review underscores the transformative potential of nitrogen-fixing cyanobacteria in promoting sustainable agriculture and enhancing global food security.

Yellow-flowered alfalfa (Medicago sativa subsp. falcata) (also known as sickle medic) has been the cornerstone for breeding alfalfa for dual grazing and hay production in the semiarid regions of the northern Great Plains in the US and Canada. Most, if not all, of the cultivars developed for the northern Great Plains during the 20th century, had parentage tracing back to introductions by Niels Ebbesen Hansen that were obtained from expeditions to Russia, primarily the province of Siberia, on behalf of the United States Department of Agriculture during the early 1900s. The M. falcata genome contains alleles for high levels of drought-tolerance, winter hardiness, and tolerance to grazing, but is generally deficient for commercial seed production traits, such as non-shatter, compared with common alfalfa (M. sativa). A naturalized population, tracing to USDA plant introductions to Perkins County South Dakota by N.E. Hansen in early 1900, and subsequently, facilitated by the determined seed increase and interseeding of a population by a local rancher, Norman ‘Bud’ Smith, has shown highly desirable in situ characteristics for improving rangelands in the northern Great Plains. This includes adequate seed production to build a seed bank in the soil for natural seedling recruitment and population maintenance/expansion and support the production of a commercial seed source. This review documents the seminal events in the development of cultivars to date and describes novel germplasm with potential for new cultivars in the future.

Climate change and disturbance can alter invasion success of clonal plants by differentially affecting the clonal traits influencing their establishment as young plants. Clonal traits related to the vegetative reproduction of native Pascopyrum smithii and non-native Bromus inermis grass seedlings were evaluated under altered precipitation frequencies and a single grazing event. Pascopyrum smithii maintained similar vegetative reproduction under three simulated precipitation frequencies whereas B. inermis vegetative reproduction declined as precipitation became more intermittent. Vegetative reproduction of the non-native B. inermis was greater than the native P. smithii under all simulated precipitation frequencies except the most intermittent scenario. A single grazing event did not affect either species’ response to intra-annual precipitation variability but did slightly reduce their clonal growth and increase their bud dormancy. In young plants, clonal traits of the invasive grass favored its superior expansion and population growth compared to the native grass except under the most severe climate change scenario. Grassland restoration using native P. smithii seeds would be successful in most years due to its resilient clonal growth in a changing climate. Clonal infrastructure development in young plants is critical to clonal plant establishment and persistence in a changing climate and under disturbed conditions.

Invasive clonal species may exhibit different growth strategies than their native clonal competitors. In this study, we examined the spatial distribution of tiller outgrowth and the bud bank by comparing the investment in phalanx versus guerilla growth of a native and invasive perennial grass in North America. We also examined the effect of altered precipitation frequency, clipping, and competition on their clonal growth strategies. Investment in phalanx and guerilla growth was assessed by examining live propagule and tiller production from the plant crown versus its rhizomes. Although invasive Bromus inermis and native Pascopyrum smithii exhibited similar clonal growth strategies as young seedlings, their clonal growth strategies significantly differed by the end of their first growing season. Pascopyrum smithii invested in dual phalanx and guerilla tiller outgrowth and bud placement, and B. inermis primarily invested in phalanx tiller outgrowth and bud placement. Competition rather than intra-annual precipitation variability and clipping altered the clonal growth strategy of these species. Intra- and inter- specific competition did not alter tiller outgrowth for either species. However, inter-specific competition caused both species to alter their bud placement. Bromus inermis shifted more buds from phalanx to guerilla positions while P. smithii shifted in the opposite direction. This may enable invasive B. inermis to expand while confining native P. smithii to more localized areas in the future. Clonal growth strategies appear to be species specific and responsive to inter-specific competition. Investigating the belowground bud aspect of clonal growth can reveal the mechanism driving the future aboveground clonal growth strategy of native and invasive rhizomatous grasses and help inform the patterns of invasion within a plant community.

Affected by the pressure and constraints of available resources, plant growth and development, as well as plant life history strategies, usually vary with environmental conditions. Plant buds play a crucial role in the life history of woody plants. Nitraria tangutorum is a common dominant woody species in desertified areas of northern China and its growth is critical to the desert ecosystem. Revealing the allometry of N. tangutorum aboveground bud fates and the linkage between bud traits and plant nutrient contents and stoichiometric ratios can be useful in understanding plant adaptation strategy. We applied seven nitrogen and phosphorus fertilizer addition treatments to natural N. tangutorum ramets in Ulan Buh Desert in three consecutive years. We surveyed three types of aboveground buds (dormant buds, vegetative buds, and reproductive buds) in each N. tangutorum ramet, then measured the plant carbon (C), nitrogen (N), and phosphorus (P) contents and ratios during three consecutive years. We specified that reserve growth potential (RGP), vegetative growth intensity (VGI) and sexual reproduction effort (SRE) are the three indices of bud dynamic pattern. The results showed that the bud dynamic pattern of N. tangutorum ramets differed significantly among different fertilizer addition treatments and sampling years. The allometry of RGP, VGI, and SRE was obvious, showing size dependence. The allometric growth relationship fluctuated among the sampling years. The linkage between bud traits and plant stoichiometric characteristics of N. tangutorum ramets showed close correlation with plant P content, C:P and N:P ratios, no significant correlation with plant C content, N content and C:N ratio. These results contribute to an improved understanding of the adaptive strategies of woody plants growing in desert ecosystems and provide insights for adoption of effective measures to restore and conserve plant communities in arid and semi-arid regions.