Perineuronal nets (PNNs) surround specific neurons in the brain and are involved in various forms of plasticity and clinical conditions. However, our understanding of the PNN role in these phenomena is limited by the lack of highly quantitative maps of PNN distribution and association with specific cell types. Here, we present a comprehensive atlas of Wisteria floribunda agglutinin (WFA)-positive PNNs and colocalization with parvalbumin (PV) cells for over 600 regions of the adult mouse brain. Data analysis shows that PV expression is a good predictor of PNN aggregation. In the cortex, PNNs are dramatically enriched in layer 4 of all primary sensory areas in correlation with thalamocortical input density, and their distribution mirrors intracortical connectivity patterns. Gene expression analysis identifies many PNN-correlated genes. Strikingly, PNN-anticorrelated transcripts are enriched in synaptic plasticity genes, generalizing PNNs' role as circuit stability factors.

Pedestrian detection through Computer Vision is a building block for a multitude of applications. Recently, there has been an increasing interest in convolutional neural network-based architectures to execute such a task. One of these supervised networks’ critical goals is to generalize the knowledge learned during the training phase to new scenarios with different characteristics. A suitably labeled dataset is essential to achieve this purpose. The main problem is that manually annotating a dataset usually requires a lot of human effort, and it is costly. To this end, we introduce ViPeD (Virtual Pedestrian Dataset), a new synthetically generated set of images collected with the highly photo-realistic graphical engine of the video game GTA V (Grand Theft Auto V), where annotations are automatically acquired. However, when training solely on the synthetic dataset, the model experiences a Synthetic2Real domain shift leading to a performance drop when applied to real-world images. To mitigate this gap, we propose two different domain adaptation techniques suitable for the pedestrian detection task, but possibly applicable to general object detection. Experiments show that the network trained with ViPeD can generalize over unseen real-world scenarios better than the detector trained over real-world data, exploiting the variety of our synthetic dataset. Furthermore, we demonstrate that with our domain adaptation techniques, we can reduce the Synthetic2Real domain shift, making the two domains closer and obtaining a performance improvement when testing the network over the real-world images.

The integration of mobile and ubiquitous computing with deep learning methods is a promising emerging trend that aims at moving the processing task closer to the data source rather than bringing the data to a central node. The advantages of this approach range from bandwidth reduction, high scalability, to high reliability, just to name a few. In this paper, we propose a real-time deep learning approach to automatically detect and count vehicles in videos taken from a UAV (Unmanned Aerial Vehicle). Our solution relies on a convolutional neural network-based model fine-tuned to the specific domain of applications that is able to precisely localize instances of the vehicles using a regression approach, straight from image pixels to bounding box coordinates, reasoning globally about the image when making predictions and implicitly encoding contextual information. A comprehensive experimental evaluation on real-world datasets shows that our approach results in state-of-the-art performances. Furthermore, our solution achieves real-time performances by running at a speed of 4 Frames Per Second on an NVIDIA Jetson TX2 board, showing the potentiality of this approach for real-time processing in UAVs.

The automatic detection of violent actions in public places through video analysis is difficult because the employed Artificial Intelligence-based techniques often suffer from generalization problems. Indeed, these algorithms hinge on large quantities of annotated data and usually experience a drastic drop in performance when used in scenarios never seen during the supervised learning phase. In this paper, we introduce and publicly release the Bus Violence benchmark, the first large-scale collection of video clips for violence detection on public transport, where some actors simulated violent actions inside a moving bus in changing conditions, such as the background or light. Moreover, we conduct a performance analysis of several state-of-the-art video violence detectors pre-trained with general violence detection databases on this newly established use case. The achieved moderate performances reveal the difficulties in generalizing from these popular methods, indicating the need to have this new collection of labeled data, beneficial for specializing them in this new scenario.