Propolis is a bee product that results from the mixture of beeswax and resinous compounds. It can scavenge free radicals; therefore, it displays antioxidant activity. Here, we have investigated the antioxidant potential of propolis produced by three Brazilian stingless bee species: Melipona quadrifasciata anthidiodes (Mandaçaia) and Tetragona clavipes (Borá), which inhabit the southeastern region of Brazil (state of São Paulo), and Scaptotrigona spp. (Tubi), which inhabits the northeastern region of Brazil (state of Maranhão). The antioxidant activities of propolis produced by different bee species were compared to the well-known antioxidant activity of green propolis, produced by the honey bee Apis mellifera. The ethanolic extract of propolis produced by Mandaçaia displayed the strongest antioxidant activity. The activity was even stronger than the antioxidant activity of green propolis. The ethyl acetate and aqueous fraction of propolis produced by Mandaçaia presented the highest antioxidant activity. This result agreed with the higher total polyphenol content verified in this propolis. high performance liquid chromatography analysis revealed that the groups of compounds present in propolis produced by Mandaçaia, Tubi, and Borá were not the same as those previously identified as the main constituents of green propolis. In fact, propolis produced by Mandaçaia contained many other polyphenols in the fingerprint, and hence its composition requires further elucidation.