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Soil structure is a crucial constituent influencing soil organic richness, rooting systems, and soil moisture conservation. Adding biochar to the soil, which directly affects aggregation, can significantly alter the soil moisture status. The extent of this impact is influenced by the temperature at which pyrolysis biochar is formed. The impact of biochar derived from wheat straw made at 350, 450, 550, and 650 °C (B₃₅₀, B₄₅₀, B₅₅₀, B₆₅₀) on soil aggregation and moisture retention was evaluated in this study. Based on the results, B₅₅₀ had the largest mean weight diameter, most water-stable aggregates, and highest available water content compared to the control, with increases of 235%, 39% and 166% compared to the control. On the other hand, B₃₅₀ was identified as the weakest treatment, with no significant difference from the control. Using B₅₅₀ and B₆₅₀ significantly reduced the soil bulk density by 13% and 12% compared to the control. Therefore, the formation of micro-aggregates, the development of soil porosity, and the subsequent increase in soil available water are unavoidable during the addition of B₅₅₀. The change in the hydrophilic character of biochar and the attainment of an optimal oxygen/carbon ratio with pyrolysis degradations is a critical factor in soil hydrology issues.