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Magnesium oxide nanoparticles (MgO-NPs) were synthesized using the fungal strain Aspergillus terreus S1 to overcome the disadvantages of chemical and physical methods. The factors affecting the biosynthesis process were optimized as follows: concentration of Mg(NO3)2·6H2O precursor (3 mM), contact time (36 min), pH (8), and incubation temperature (35 °C). The characterization of biosynthesized MgO-NPs was accomplished using UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy—energy dispersive X-ray (SEM-EDX), X-ray diffraction (XRD), and dynamic light scattering (DLS). Data confirmed the successful formation of crystallographic, spherical, well-dispersed MgO-NPs with a size range of 8.0–38.0 nm at a maximum surface plasmon resonance of 280 nm. The biological activities of biosynthesized MgO-NPs including antimicrobial activity, biotreatment of tanning effluent, and chromium ion removal were investigated. The highest growth inhibition of pathogenic Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans was achieved at 200 μg mL–1 of MgO-NPs. The biosynthesized MgO-NPs exhibited high efficacy to decolorize the tanning effluent (96.8 ± 1.7% after 150 min at 1.0 µg mL–1) and greatly decrease chemical parameters including total suspended solids (TSS), total dissolved solids (TDS), biological oxygen demand (BOD), chemical oxygen demand (COD), and conductivity with percentages of 98.04, 98.3, 89.1, 97.2, and 97.7%, respectively. Further, the biosynthesized MgO-NPs showed a strong potential to remove chromium ions from the tanning effluent, from 835.3 mg L–1 to 21.0 mg L–1, with a removal percentage of 97.5%.
Ebrahim Saied; Ahmed Eid; Saad Hassan; Salem Salem; Ahmed Radwan; Mahmoud Halawa; Fayez Saleh; Hosam Saad; Essa Saied; Amr Fouda. The Catalytic Activity of Biosynthesized Magnesium Oxide Nanoparticles (MgO-NPs) for Inhibiting the Growth of Pathogenic Microbes, Tanning Effluent Treatment, and Chromium Ion Removal. Catalysts 2021, 11, 821 .
AMA StyleEbrahim Saied, Ahmed Eid, Saad Hassan, Salem Salem, Ahmed Radwan, Mahmoud Halawa, Fayez Saleh, Hosam Saad, Essa Saied, Amr Fouda. The Catalytic Activity of Biosynthesized Magnesium Oxide Nanoparticles (MgO-NPs) for Inhibiting the Growth of Pathogenic Microbes, Tanning Effluent Treatment, and Chromium Ion Removal. Catalysts. 2021; 11 (7):821.
Chicago/Turabian StyleEbrahim Saied; Ahmed Eid; Saad Hassan; Salem Salem; Ahmed Radwan; Mahmoud Halawa; Fayez Saleh; Hosam Saad; Essa Saied; Amr Fouda. 2021. "The Catalytic Activity of Biosynthesized Magnesium Oxide Nanoparticles (MgO-NPs) for Inhibiting the Growth of Pathogenic Microbes, Tanning Effluent Treatment, and Chromium Ion Removal." Catalysts 11, no. 7: 821.
The discovery of eco-friendly, rapid, and cost-effective compounds to control diseases caused by microbes and insects are the main challenges. Herein, the magnesium oxide nanoparticles (MgO-NPs) are successfully fabricated by harnessing the metabolites secreted by Penicillium chrysogenum. The fabricated MgO-NPs were characterized using UV-Vis, XRD, TEM, DLS, EDX, FT-IR, and XPS analyses. Data showed the successful formation of crystallographic, spherical, well-dispersed MgO-NPs with sizes of 7–40 nm at a maximum wavelength of 250 nm. The EDX analysis confirms the presence of Mg and O ions as the main components with weight percentages of 13.62% and 7.76%, respectively. The activity of MgO-NPs as an antimicrobial agent was investigated against pathogens Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans, and exhibited zone of inhibitions of 12.0 ± 0.0, 12.7 ± 0.9, 23.3 ± 0.8, 17.7 ± 1.6, and 14.7 ± 0.6 mm respectively, at 200 µg mL−1. The activity is decreased by decreasing the MgO-NPs concentration. The biogenic MgO-NPs exhibit high efficacy against different larvae instar and pupa of Anopheles stephensi, with LC50 values of 12.5–15.5 ppm for I–IV larvae instar and 16.5 ppm for the pupa. Additionally, 5 mg/cm2 of MgO-NPs showed the highest protection percentages against adults of Anopheles stephensi, with values of 100% for 150 min and 67.6% ± 1.4% for 210 min.
Amr Fouda; Mohamed A. Awad; Ahmed M. Eid; Ebrahim Saied; Mohammed G. Barghoth; Mohammed F. Hamza; Salah Abdelbary; Saad El-Din Hassan. An Eco-Friendly Approach to the Control of Pathogenic Microbes and Anopheles stephensi Malarial Vector Using Magnesium Oxide Nanoparticles (Mg-NPs) Fabricated by Penicillium chrysogenum. International Journal of Molecular Sciences 2021, 22, 5096 .
AMA StyleAmr Fouda, Mohamed A. Awad, Ahmed M. Eid, Ebrahim Saied, Mohammed G. Barghoth, Mohammed F. Hamza, Salah Abdelbary, Saad El-Din Hassan. An Eco-Friendly Approach to the Control of Pathogenic Microbes and Anopheles stephensi Malarial Vector Using Magnesium Oxide Nanoparticles (Mg-NPs) Fabricated by Penicillium chrysogenum. International Journal of Molecular Sciences. 2021; 22 (10):5096.
Chicago/Turabian StyleAmr Fouda; Mohamed A. Awad; Ahmed M. Eid; Ebrahim Saied; Mohammed G. Barghoth; Mohammed F. Hamza; Salah Abdelbary; Saad El-Din Hassan. 2021. "An Eco-Friendly Approach to the Control of Pathogenic Microbes and Anopheles stephensi Malarial Vector Using Magnesium Oxide Nanoparticles (Mg-NPs) Fabricated by Penicillium chrysogenum." International Journal of Molecular Sciences 22, no. 10: 5096.
The metabolites of the fungal strain Rhizopus oryaze were used as a biocatalyst for the green-synthesis of magnesium oxide nanoparticles (MgO-NPs). The production methodology was optimized to attain the maximum productivity as follows: 4 mM of precursor, at pH 8, incubation temperature of 35 °C, and reaction time of 36 h between metabolites and precursor. The as-formed MgO-NPs were characterized by UV-Vis spectroscopy, TEM, SEM-EDX, XRD, DLS, FT-IR, and XPS analyses. These analytical techniques proved to gain crystalline, homogenous, and well-dispersed spherical MgO-NPs with an average size of 20.38 ± 9.9 nm. The potentiality of MgO-NPs was dose- and time-dependent. The biogenic MgO-NPs was found to be a promising antimicrobial agent against the pathogens including Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans with inhibition zones of 10.6 ± 0.4, 11.5 ± 0.5, 13.7 ± 0.5, 14.3 ± 0.7, and 14.7 ± 0.6 mm, respectively, at 200 μg mL–1. Moreover, MgO-NPs manifested larvicidal and adult repellence activity against Culex pipiens at very low concentrations. The highest decolorization percentages of tanning effluents were 95.6 ± 1.6% at 100 µg/ 100 mL after 180 min. At this condition, the physicochemical parameters of tannery effluents, including TSS, TDS, BOD, COD, and conductivity were reduced with percentages of 97.9%, 98.2%, 87.8%, 95.9%, and 97.3%, respectively. Moreover, the chromium ion was adsorbed with percentages of 98.2% at optimum experimental conditions.
Saad El-Din Hassan; Amr Fouda; Ebrahim Saied; Mohamed M. S. Farag; Ahmed M. Eid; Mohammed G. Barghoth; Mohamed A. Awad; Mohammed F. Hamza. Rhizopus oryzae-Mediated Green Synthesis of Magnesium Oxide Nanoparticles (MgO-NPs): A Promising Tool for Antimicrobial, Mosquitocidal Action, and Tanning Effluent Treatment. Journal of Fungi 2021, 7, 372 .
AMA StyleSaad El-Din Hassan, Amr Fouda, Ebrahim Saied, Mohamed M. S. Farag, Ahmed M. Eid, Mohammed G. Barghoth, Mohamed A. Awad, Mohammed F. Hamza. Rhizopus oryzae-Mediated Green Synthesis of Magnesium Oxide Nanoparticles (MgO-NPs): A Promising Tool for Antimicrobial, Mosquitocidal Action, and Tanning Effluent Treatment. Journal of Fungi. 2021; 7 (5):372.
Chicago/Turabian StyleSaad El-Din Hassan; Amr Fouda; Ebrahim Saied; Mohamed M. S. Farag; Ahmed M. Eid; Mohammed G. Barghoth; Mohamed A. Awad; Mohammed F. Hamza. 2021. "Rhizopus oryzae-Mediated Green Synthesis of Magnesium Oxide Nanoparticles (MgO-NPs): A Promising Tool for Antimicrobial, Mosquitocidal Action, and Tanning Effluent Treatment." Journal of Fungi 7, no. 5: 372.
Endophytic bacteria colonize plants and live inside them for part of or throughout their life without causing any harm or disease to their hosts. The symbiotic relationship improves the physiology, fitness, and metabolite profile of the plants, while the plants provide food and shelter for the bacteria. The bacteria-induced alterations of the plants offer many possibilities for biotechnological, medicinal, and agricultural applications. The endophytes promote plant growth and fitness through the production of phytohormones or biofertilizers, or by alleviating abiotic and biotic stress tolerance. Strengthening of the plant immune system and suppression of disease are associated with the production of novel antibiotics, secondary metabolites, siderophores, and fertilizers such as nitrogenous or other industrially interesting chemical compounds. Endophytic bacteria can be used for phytoremediation of environmental pollutants or the control of fungal diseases by the production of lytic enzymes such as chitinases and cellulases, and their huge host range allows a broad spectrum of applications to agriculturally and pharmaceutically interesting plant species. More recently, endophytic bacteria have also been used to produce nanoparticles for medical and industrial applications. This review highlights the biotechnological possibilities for bacterial endophyte applications and proposes future goals for their application.
Ahmed Eid; Amr Fouda; Mohamed Abdel-Rahman; Salem Salem; Albaraa Elsaied; Ralf Oelmüller; Mohamed Hijri; Arnab Bhowmik; Amr Elkelish; Saad Hassan. Harnessing Bacterial Endophytes for Promotion of Plant Growth and Biotechnological Applications: An Overview. Plants 2021, 10, 935 .
AMA StyleAhmed Eid, Amr Fouda, Mohamed Abdel-Rahman, Salem Salem, Albaraa Elsaied, Ralf Oelmüller, Mohamed Hijri, Arnab Bhowmik, Amr Elkelish, Saad Hassan. Harnessing Bacterial Endophytes for Promotion of Plant Growth and Biotechnological Applications: An Overview. Plants. 2021; 10 (5):935.
Chicago/Turabian StyleAhmed Eid; Amr Fouda; Mohamed Abdel-Rahman; Salem Salem; Albaraa Elsaied; Ralf Oelmüller; Mohamed Hijri; Arnab Bhowmik; Amr Elkelish; Saad Hassan. 2021. "Harnessing Bacterial Endophytes for Promotion of Plant Growth and Biotechnological Applications: An Overview." Plants 10, no. 5: 935.
Microbial endophytes organize symbiotic relationships with the host plant, and their excretions contain diverse plant beneficial matter such as phytohormones and bioactive compounds. In the present investigation, six bacterial and four fungal strains were isolated from the common bean (Phaseolus vulgaris L.) root plant, identified using molecular techniques, and their growth-promoting properties were reviewed. All microbial isolates showed varying activities to produce indole-3-acetic acid (IAA) and different hydrolytic enzymes such as amylase, cellulase, protease, pectinase, and xylanase. Six bacterial endophytic isolates displayed phosphate-solubilizing capacity and ammonia production. We conducted a field experiment to evaluate the promotion activity of the metabolites of the most potent endophytic bacterial (Bacillus thuringiensis PB2 and Brevibacillus agri PB5) and fungal (Alternaria sorghi PF2 and, Penicillium commune PF3) strains in comparison to two exogenously applied hormone, IAA, and benzyl adenine (BA), on the growth and biochemical characteristics of the P. vulgaris L. Interestingly, our investigations showed that bacterial and fungal endophytic metabolites surpassed the exogenously applied hormones in increasing the plant biomass, photosynthetic pigments, carbohydrate and protein contents, antioxidant enzyme activity, endogenous hormones and yield traits. Our findings illustrate that the endophyte Brevibacillus agri (PB5) provides high potential as a stimulator for the growth and productivity of common bean plants.
Mohamed Ismail; Mohamed Amin; Ahmed Eid; Saad Hassan; Hany Mahgoub; Islam Lashin; Abdelrhman Abdelwahab; Ehab Azab; Adil Gobouri; Amr Elkelish; Amr Fouda. Comparative Study between Exogenously Applied Plant Growth Hormones versus Metabolites of Microbial Endophytes as Plant Growth-Promoting for Phaseolus vulgaris L. Cells 2021, 10, 1059 .
AMA StyleMohamed Ismail, Mohamed Amin, Ahmed Eid, Saad Hassan, Hany Mahgoub, Islam Lashin, Abdelrhman Abdelwahab, Ehab Azab, Adil Gobouri, Amr Elkelish, Amr Fouda. Comparative Study between Exogenously Applied Plant Growth Hormones versus Metabolites of Microbial Endophytes as Plant Growth-Promoting for Phaseolus vulgaris L. Cells. 2021; 10 (5):1059.
Chicago/Turabian StyleMohamed Ismail; Mohamed Amin; Ahmed Eid; Saad Hassan; Hany Mahgoub; Islam Lashin; Abdelrhman Abdelwahab; Ehab Azab; Adil Gobouri; Amr Elkelish; Amr Fouda. 2021. "Comparative Study between Exogenously Applied Plant Growth Hormones versus Metabolites of Microbial Endophytes as Plant Growth-Promoting for Phaseolus vulgaris L." Cells 10, no. 5: 1059.
Endophytic fungi are widely present in internal plant tissues and provide different benefits to their host. Medicinal plants have unexplored diversity of functional fungal association; therefore, this study aimed to isolate endophytic fungi associated with leaves of medicinal plants Ephedra pachyclada and evaluate their plant growth-promoting properties. Fifteen isolated fungal endophytes belonging to Ascomycota, with three different genera, Penicillium, Alternaria, and Aspergillus, were obtained from healthy leaves of E. pachyclada. These fungal endophytes have varied antimicrobial activity against human pathogenic microbes and produce ammonia and indole acetic acid (IAA), in addition to their enzymatic activity. The results showed that Penicillium commune EP-5 had a maximum IAA productivity of 192.1 ± 4.04 µg mL−1 in the presence of 5 µg mL−1 tryptophan. The fungal isolates of Penicillium crustosum EP-2, Penicillium chrysogenum EP-3, and Aspergillus flavus EP-14 exhibited variable efficiency for solubilizing phosphate salts. Five representative fungal endophytes of Penicillium crustosum EP-2, Penicillium commune EP-5, Penicillium caseifulvum EP-11, Alternaria tenuissima EP-13, and Aspergillus flavus EP-14 and their consortium were selected and applied as bioinoculant to maize plants. The results showed that Penicillium commune EP-5 increased root lengths from 15.8 ± 0.8 to 22.1 ± 0.6. Moreover, the vegetative growth features of inoculated maize plants improved more than the uninoculated ones.
Ahmed Khalil; Saad Hassan; Sultan Alsharif; Ahmed Eid; Emad Ewais; Ehab Azab; Adil Gobouri; Amr Elkelish; Amr Fouda. Isolation and Characterization of Fungal Endophytes Isolated from Medicinal Plant Ephedra pachyclada as Plant Growth-Promoting. Biomolecules 2021, 11, 140 .
AMA StyleAhmed Khalil, Saad Hassan, Sultan Alsharif, Ahmed Eid, Emad Ewais, Ehab Azab, Adil Gobouri, Amr Elkelish, Amr Fouda. Isolation and Characterization of Fungal Endophytes Isolated from Medicinal Plant Ephedra pachyclada as Plant Growth-Promoting. Biomolecules. 2021; 11 (2):140.
Chicago/Turabian StyleAhmed Khalil; Saad Hassan; Sultan Alsharif; Ahmed Eid; Emad Ewais; Ehab Azab; Adil Gobouri; Amr Elkelish; Amr Fouda. 2021. "Isolation and Characterization of Fungal Endophytes Isolated from Medicinal Plant Ephedra pachyclada as Plant Growth-Promoting." Biomolecules 11, no. 2: 140.
In this study, 15 bacterial endophytes linked with the leaves of the native medicinal plant Pulicaria incisa were isolated and identified as Agrobacterium fabrum, Acinetobacter radioresistant, Brevibacillus brevis, Bacillus cereus, Bacillus subtilis, Paenibacillus barengoltzii, and Burkholderia cepacia. These isolates exhibited variant tolerances to salt stress and showed high efficacy in indole-3-acetic acid (IAA) production in the absence/presence of tryptophan. The maximum productivity of IAA was recorded for B. cereus BI-8 and B. subtilis BI-10 with values of 117 ± 6 and 108 ± 4.6 μg mL−1, respectively, in the presence of 5 mg mL−1 tryptophan after 10 days. These two isolates had a high potential in phosphate solubilization and ammonia production, and they showed enzymatic activities for amylase, protease, xylanase, cellulase, chitinase, and catalase. In vitro antagonistic investigation showed their high efficacy against the three phytopathogens Fusarium oxysporum, Alternaria alternata, and Pythium ultimum, with inhibition percentages ranging from 20% ± 0.2% to 52.6% ± 0.2% (p ≤ 0.05). Therefore, these two endophytic bacteria were used as bio-inoculants for maize seeds, and the results showed that bacterial inoculations significantly increased the root length as well as the fresh and dry weights of the roots compared to the control plants. The Zea mays plant inoculated with the two endophytic strains BI-8 and BI-10 significantly improved (p ≤ 0.05) the growth performance as well as the nutrient uptake compared with an un-inoculated plant.
Amr Fouda; Ahmed M. Eid; Albaraa Elsaied; Ehab F. El-Belely; Mohammed G. Barghoth; Ehab Azab; Adil A. Gobouri; Saad El-Din Hassan. Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of Pulicaria incisa (Lam.) DC Inherent to Arid Regions. Plants 2021, 10, 76 .
AMA StyleAmr Fouda, Ahmed M. Eid, Albaraa Elsaied, Ehab F. El-Belely, Mohammed G. Barghoth, Ehab Azab, Adil A. Gobouri, Saad El-Din Hassan. Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of Pulicaria incisa (Lam.) DC Inherent to Arid Regions. Plants. 2021; 10 (1):76.
Chicago/Turabian StyleAmr Fouda; Ahmed M. Eid; Albaraa Elsaied; Ehab F. El-Belely; Mohammed G. Barghoth; Ehab Azab; Adil A. Gobouri; Saad El-Din Hassan. 2021. "Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of Pulicaria incisa (Lam.) DC Inherent to Arid Regions." Plants 10, no. 1: 76.
An endophytic strain of Streptomyces antimycoticus L-1 was isolated from healthy medicinal plant leaves of Mentha longifolia L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV–vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs with a particle size of 13–40 nm. Further, the stability of the Ag-NPs was assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month or by measuring the NPs surface charge (−19.2 mV) by zeta potential analysis (ζ). The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations (6.25–100 ppm) against the pathogens Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium with a clear inhibition zone ranging from (9.5 ± 0.4) nm to (21.7 ± 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC50) = 5.7 ± 0.2 ppm). With respect to antibacterial and in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected as a safe dose for loading onto cotton fabrics. The scanning electron microscopy connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric elements. Moreover, the nano-finished fabrics exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing cycles, indicating the stability of the treated fabrics.
Salem S. Salem; Ehab F. El-Belely; Gniewko Niedbała; Maryam M. Alnoman; Saad El-Din Hassan; Ahmed Mohamed Eid; Tharwat I. Shaheen; Amr Elkelish; Amr Fouda. Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics. Nanomaterials 2020, 10, 2082 .
AMA StyleSalem S. Salem, Ehab F. El-Belely, Gniewko Niedbała, Maryam M. Alnoman, Saad El-Din Hassan, Ahmed Mohamed Eid, Tharwat I. Shaheen, Amr Elkelish, Amr Fouda. Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics. Nanomaterials. 2020; 10 (10):2082.
Chicago/Turabian StyleSalem S. Salem; Ehab F. El-Belely; Gniewko Niedbała; Maryam M. Alnoman; Saad El-Din Hassan; Ahmed Mohamed Eid; Tharwat I. Shaheen; Amr Elkelish; Amr Fouda. 2020. "Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics." Nanomaterials 10, no. 10: 2082.
Improvement of the medical textile industry has received more attention recently, especially with widespread of microbial and viral infections. Medical textiles with new properties, such as bacterial pathogens self-cleaning, have been explored with nanotechnology. In this study, an endophytic actinomycetes strain of Streptomyces laurentii R-1 was isolated from the roots of the medicinal plant Achillea fragrantissima. This is used as a catalyst for the mediated biosynthesis of silver nanoparticles (Ag-NPs) for applications in the textile industry. The biosynthesized Ag-NPs were characterized using UV-vis spectroscopy, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), and X-ray Diffraction (XRD), which confirmed the successful formation of crystalline, spherical metal nanoparticles. The biosynthesized Ag-NPs exhibited broad-spectrum antibacterial activity. Our data elucidated that the biosynthesized Ag-NPs had a highly cytotoxic effect against the cancerous caco-2 cell line. The selected safe dose of Ag-NPs for loading on cotton fabrics was 100 ppm, regarding their antibacterial activity and safe cytotoxic efficacy. Interestingly, scanning electron microscope connected with energy dispersive X-ray spectroscopy (SEM-EDX) of loaded cotton fabrics demonstrated the smooth distribution of Ag-NPs on treated fabrics. The obtained results highlighted the broad-spectrum activity of nano-finished fabrics against pathogenic bacteria, even after 5 and 10 washing cycles. This study contributes a suitable guide for the performance of green synthesized NPs for utilization in different biotechnological sectors.
Ahmed M. Eid; Amr Fouda; Gniewko Niedbała; Saad El-Din Hassan; Salem S. Salem; Abdullah M. Abdo; Helal F. Hetta; Tharwat I. Shaheen. Endophytic Streptomyces laurentii Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties. Antibiotics 2020, 9, 641 .
AMA StyleAhmed M. Eid, Amr Fouda, Gniewko Niedbała, Saad El-Din Hassan, Salem S. Salem, Abdullah M. Abdo, Helal F. Hetta, Tharwat I. Shaheen. Endophytic Streptomyces laurentii Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties. Antibiotics. 2020; 9 (10):641.
Chicago/Turabian StyleAhmed M. Eid; Amr Fouda; Gniewko Niedbała; Saad El-Din Hassan; Salem S. Salem; Abdullah M. Abdo; Helal F. Hetta; Tharwat I. Shaheen. 2020. "Endophytic Streptomyces laurentii Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties." Antibiotics 9, no. 10: 641.
Desert plants are able to survive under harsh environmental stresses inherent to arid and semiarid regions due to their association with bacterial endophytes. However, the identity, functions, and the factors that influence the association of bacterial endophytes with desert plants are poorly known. These bacterial endophytes can be used as an untapped resource to favor plant growth and development in agro-ecosystems of arid regions. The present study is therefore focused on the isolation and identification of bacterial endophytes from two native medicinal plants (Fagonia mollis Delile and Achillea fragrantissima (Forssk) Sch. Bip.) growing spontaneously in the arid region of the South Sinai (Egypt), and characterization of their plant growth promoting (PGP) traits. Thirteen putative bacterial endophytes were isolated from the leaves of both plant species and characterized for their plant growth promoting abilities using molecular and biochemical approaches, as well as greenhouse trials. Selected endophytic bacterial strains were applied to maize plants (Zea mays L. var. Single cross Pioneer 30K08) to further evaluate their PGP abilities under greenhouse conditions. Isolated bacterial strains have variable plant growth promoting activities. Among these activities, isolated bacterial endophytes have the efficacy of phosphate solubilizing with clear zones ranging from 7.6 ± 0.3 to 9.6 ± 0.3 mm. Additionally, the obtained bacterial endophytes increased the productivity of indole acetic acid (IAA) in broth media from 10 to 60 µg·mL−1 with increasing tryptophan concentration from 1 to 5 mg·mL−1. Bacillus and Brevibacillus strains were frequently isolated from the leaves of both plant species, and had significant positive effects on plant growth and shoot phosphorus (P) and nitrogen (N) contents. Results suggest that these endophytes are good candidates as plant growth promoting inoculants to help reduce chemical input in conventional agricultural practices and increase nutrient uptake and stress resilience in plant species.
Muneera D. F. Alkahtani; Amr Fouda; Kotb A. Attia; Fahad Al-Otaibi; Ahmed M. Eid; Emad El-Din Ewais; Mohamed Hijri; Marc St-Arnaud; Saad El-Din Hassan; Naeem Khan; Yaser M. Hafez; Khaled A. A. Abdelaal. Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture. Agronomy 2020, 10, 1325 .
AMA StyleMuneera D. F. Alkahtani, Amr Fouda, Kotb A. Attia, Fahad Al-Otaibi, Ahmed M. Eid, Emad El-Din Ewais, Mohamed Hijri, Marc St-Arnaud, Saad El-Din Hassan, Naeem Khan, Yaser M. Hafez, Khaled A. A. Abdelaal. Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture. Agronomy. 2020; 10 (9):1325.
Chicago/Turabian StyleMuneera D. F. Alkahtani; Amr Fouda; Kotb A. Attia; Fahad Al-Otaibi; Ahmed M. Eid; Emad El-Din Ewais; Mohamed Hijri; Marc St-Arnaud; Saad El-Din Hassan; Naeem Khan; Yaser M. Hafez; Khaled A. A. Abdelaal. 2020. "Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture." Agronomy 10, no. 9: 1325.
Salinity stress deleteriously affects the growth and yield of many plants. Plant growth promoting rhizobacteria (PGPR) and chitosan both play an important role in combating salinity stress and improving plant growth under adverse environmental conditions. The present study aimed to evaluate the impacts of PGPR and chitosan on the growth of sweet pepper plant grown under different salinity regimes. For this purpose, two pot experiments were conducted in 2019 and 2020 to evaluate the role of PGPR (Bacillus thuringiensis MH161336 106–8 CFU/cm3) applied as seed treatment and foliar application of chitosan (30 mg dm−3) on sweet pepper plants (cv. Yolo Wonder) under two salinity concentrations (34 and 68 mM). Our findings revealed that, the chlorophyll fluorescence parameter (Fv/Fm ratio), chlorophyll a and b concentrations, relative water content (RWC), and fruit yield characters were negatively affected and significantly reduced under salinity conditions. The higher concentration was more harmful. Nevertheless, electrolyte leakage, lipid peroxidation, hydrogen peroxide (H2O2), and superoxide (O2−) significantly increased in stressed plants. However, the application of B. thuringiensis and chitosan led to improved plant growth and resulted in a significant increase in RWC, chlorophyll content, chlorophyll fluorescence parameter (Fv/Fm ratio), and fruit yield. Conversely, lipid peroxidation, electrolyte leakage, O2−, and H2O2 were significantly reduced in stressed plants. Also, B. thuringiensis and chitosan application regulated the proline accumulation and enzyme activity, as well as increased the number of fruit plant−1, fruit fresh weight plant−1, and total fruit yield of sweet pepper grown under saline conditions
Muneera D. F. Alkahtani; M. Hafez; Naeem Khan; Ahmed Eid; Mohamed A. M. Ali; Khaled Abdelaal. Chlorophyll Fluorescence Parameters and Antioxidant Defense System Can Display Salt Tolerance of Salt Acclimated Sweet Pepper Plants Treated with Chitosan and Plant Growth Promoting Rhizobacteria. Agronomy 2020, 10, 1180 .
AMA StyleMuneera D. F. Alkahtani, M. Hafez, Naeem Khan, Ahmed Eid, Mohamed A. M. Ali, Khaled Abdelaal. Chlorophyll Fluorescence Parameters and Antioxidant Defense System Can Display Salt Tolerance of Salt Acclimated Sweet Pepper Plants Treated with Chitosan and Plant Growth Promoting Rhizobacteria. Agronomy. 2020; 10 (8):1180.
Chicago/Turabian StyleMuneera D. F. Alkahtani; M. Hafez; Naeem Khan; Ahmed Eid; Mohamed A. M. Ali; Khaled Abdelaal. 2020. "Chlorophyll Fluorescence Parameters and Antioxidant Defense System Can Display Salt Tolerance of Salt Acclimated Sweet Pepper Plants Treated with Chitosan and Plant Growth Promoting Rhizobacteria." Agronomy 10, no. 8: 1180.
This study addresses the impacts of metabolites from different microbial taxa on the fabrication and multifunctional biological properties of spherical silver nanoparticles (Ag-NPs). Three microbial taxa, a bacterial (Bacillus cereus A1-5), actinomycetes (Streptomyces noursei H1-1), and fungal (Rhizopus stolonifer A6-2) strains were used for Ag-NPs biosynthesis, whereas Streptomyces noursei is demonstrated for the first time. These isolates were identified using either 16S rRNA or ITS gene sequencing. Characterization of Ag-NPs was done using color change analysis, Uv-Vis spectroscopy, FT-IR spectroscopy, XRD, TEM, SEM-EDX, DLS, and Zeta potential analysis. All biosynthesized NPs exhibited spherical shape with different sizes ranged from 6‒50 nm, 6-30 nm and 6-40 nm for NPs obtained by A1-5, H1-1 and A6-2, respectively. The crystalline center cubic face of Ag-NPs was confirmed using XRD at 2θ values 38.08o, 44.27o, 64.41o and 77.36o. FT-IR analysis revealed varied intense absorption peaks for biomolecules required for NPs synthesize by each microbial strain. The stability of spherical Ag-NPs was confirmed due to highly DLS negative surface charge of ‒17.5mV, ‒18.9mV, and ‒15.6mV for NPs synthesized by strains A1-5, H1-1, and A6-2, respectively. Ag-NPs exhibited a broadspectrum of antibacterial activity against Gram-positive and Gram-negative bacteria with varied effectiveness. They also exhibited a cytotoxic effect against cancer cell line (caco-2) in a dose-dependent pattern with IC50 of 8.9 ± 0.5, 5.6 ± 3.0, 11.2 ± 0.5 μg/ml for NPs synthesized by strains A1-5, H1-1, and A6-2, respectively. Moreover, these spherical Ag-NPs showed larvicidal activity against the 3rd instar larvae of the dengue vector Aedes aegypti.
Sultan M. Alsharif; Salem S. Salem; Mohamed Ali Abdel-Rahman; Amr Fouda; Ahmed Eid; Saad El-Din Hassan; Mohamed A. Awad; Asem A. Mohamed. Multifunctional properties of spherical silver nanoparticles fabricated by different microbial taxa. Heliyon 2020, 6, e03943 .
AMA StyleSultan M. Alsharif, Salem S. Salem, Mohamed Ali Abdel-Rahman, Amr Fouda, Ahmed Eid, Saad El-Din Hassan, Mohamed A. Awad, Asem A. Mohamed. Multifunctional properties of spherical silver nanoparticles fabricated by different microbial taxa. Heliyon. 2020; 6 (5):e03943.
Chicago/Turabian StyleSultan M. Alsharif; Salem S. Salem; Mohamed Ali Abdel-Rahman; Amr Fouda; Ahmed Eid; Saad El-Din Hassan; Mohamed A. Awad; Asem A. Mohamed. 2020. "Multifunctional properties of spherical silver nanoparticles fabricated by different microbial taxa." Heliyon 6, no. 5: e03943.
Historic cultural heritage and archival manuscripts have a significant importance worldwide and efforts should be considered to preserve such property for as long as possible. The current study aimed to establish a non-toxic, and eco-friendly conservation strategy for retarding paper biodeterioration. To this end, fungal isolates associated with the biodeteriorated paper manuscripts of the historical book “DESCRIPTION DE L´EGYPTE” dating back to 1823 A.D were isolated. These isolates were identified as Asperigllus niger, Aspergillus flavus, Penicillium chrysogenum and Alternaria alternata and then evaluated for cellulolytic activities. Amongst them, Aspergillus niger A2 exhibited the highest cellulase activity. In in vitro study using filter papers as model, the suppressive effect of biosynthesized silver and zinc oxide nanoparticles (NPs) on A. niger A2 bioderteriorant activity was evaluated. Biosynthesized Ag-NPs and ZnO-NPs were characterized by UV-Vis spectroscopy, X-ray diffraction patterns (XRD), Transmission Electron Microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FT-IR). Color changes, tensile strength, FT-IR-spectroscopy, and scanning electron microscopy of NPs-treated papers were examined. Treatments with 2 mM Ag-NPs and 2 mM ZnO-NPs exhibited the best conservation effect on treated papers and alleviated cellulose degradation. Furthermore, this treatments enhanced tensile strength and preserved the original morphology of paper fibers. These results introduced a potential approach for historical documents conservation against fungal biodeterioration through NPs sprays. It can be also used for preparing anti-biodeterioration paper for expected manuscripts uses.
Amr Fouda; Gomaa Abdel-Maksoud; Mohamed Ali Abdel-Rahman; Ahmed Eid; Mohammed G. Barghoth; Mohamad Abdel-Haleem El-Sadany. Monitoring the effect of biosynthesized nanoparticles against biodeterioration of cellulose-based materials by Aspergillus niger. Cellulose 2019, 26, 6583 -6597.
AMA StyleAmr Fouda, Gomaa Abdel-Maksoud, Mohamed Ali Abdel-Rahman, Ahmed Eid, Mohammed G. Barghoth, Mohamad Abdel-Haleem El-Sadany. Monitoring the effect of biosynthesized nanoparticles against biodeterioration of cellulose-based materials by Aspergillus niger. Cellulose. 2019; 26 (11):6583-6597.
Chicago/Turabian StyleAmr Fouda; Gomaa Abdel-Maksoud; Mohamed Ali Abdel-Rahman; Ahmed Eid; Mohammed G. Barghoth; Mohamad Abdel-Haleem El-Sadany. 2019. "Monitoring the effect of biosynthesized nanoparticles against biodeterioration of cellulose-based materials by Aspergillus niger." Cellulose 26, no. 11: 6583-6597.
Fungal endophytes associated with medicinal plants have potential role to promote plant growth through different mechanisms. However, the biological and ecological roles of fungal endophytes still totally unexplored. In this study, three different fungal endophytes were isolated from the medicinal plant of Asclepias sinaica and identified as Penicillium chrysogenum Pc_25, Alternaria alternata Aa_27 and the third fungal strain was described as sterile hyphae Sh_26. It was recorded that, these endophytes had various ability to produce several extracellular enzymes including amylase, pectinase, cellulase, gelatinase, xylanase and tyrosinase. Their antimicrobial activities against different specific test organisms were investigated as well. In addition, both endophyte isolates i.e. Sh_26 and Aa_27 were found to promote root growth higher than Pc_25 and control treatments. These fungal isolates had a considerable impact on plant growth parameters including root elongation as a result of ammonia and IAA production
Amr Fouda; Saad El-Din Hassan; Ahmed Eid; Emad El-Din Ewais. Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.). Annals of Agricultural Sciences 2015, 60, 95 -104.
AMA StyleAmr Fouda, Saad El-Din Hassan, Ahmed Eid, Emad El-Din Ewais. Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.). Annals of Agricultural Sciences. 2015; 60 (1):95-104.
Chicago/Turabian StyleAmr Fouda; Saad El-Din Hassan; Ahmed Eid; Emad El-Din Ewais. 2015. "Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.)." Annals of Agricultural Sciences 60, no. 1: 95-104.