This study aimed to investigate the effects of feeding dairy calves starter diets containing corn grain processed by different methods (ground versus steam-flaked; GRC vs. SFC) and either 18% or 21% crude protein (CP) contents (dry matter basis) on growth performance, digestibility, ruminal fermentation, urinary purine derivatives, and blood metabolites of dairy calves. Holstein dairy calves (39.3 ± 1.9 kg of body weight, n = 12 calves per treatment, 6 males and 6 females) were randomly distributed to experimental diets in a 2 × 2 factorial arrangement of treatments. The 4 dietary treatments were (1) starter diet containing GRC and 18% dietary CP (GRC-18CP; geometric mean particle size, GMPS = 0.73 mm); (2) GRC and 21% dietary CP (GRC-21CP; GMPS = 0.71 mm); (3) SFC and 18% dietary CP (SFC-18CP; GMPS = 2.21 mm); and (4) SFC and 21% dietary CP (SFC-21CP; GMPS = 2.16 mm). Calves were weaned on d 63 and remained in the study until d 83 of age. The starter feed intake did not differ among treatments; however, average daily gain and feed efficiency (FE) were improved and final body weight was higher for SFC diets compared with GRC diets. The organic matter and nonfiber carbohydrate digestibilities were greater for calves fed the SFC diets than for those fed the GRC diets. The ruminal total volatile fatty acid concentrations and the molar proportions of propionate and butyrate were greater, and the molar proportion of acetate and NH₃-N concentrations were lower, for calves fed the SFC diets than for those fed the GRC diets. The excretion of allantoin and total purine derivatives, and subsequently microbial protein synthesis, were greater for calves fed the SFC diets than the GRC diets. The total urinary nitrogen excretion and its proportion of N intake were lower for calves fed the SFC diets than the GRC diets. The blood concentrations of insulin (pre- and postweaning), glucose (postweaning), and β-hydroxybutyrate (preweaning) were greater and blood urea nitrogen (preweaning) was lower for calves fed the SFC diets than the GRC diets. The protein content of the concentrate did not affect feed intake, growth performance, or ruminal fermentation of the calves. The neutral detergent fiber digestibility was greater for calves fed the 21% CP diets than the 18% CP diets. No interaction between main effects was observed regarding the starter intake, average daily gain, body weight, FE, ruminal fermentation, and nutrient digestibility of calves. The interaction between corn grain processing and starter protein content was significant for withers and hip heights with the greatest values found for SFC-21CP treatment. Our results show that steam flaking of corn improved the organic matter and nonfiber carbohydrate digestibilities, weight gain, FE, and ruminal microbial protein synthesis, and reduced urinary nitrogen excretion compared with grinding corn. Regardless of the marginal benefit derived from feeding the diet containing SFC and 21% CP in the height of calves, lower starter protein content (18% CP) may be used efficiently when calves are fed the SFC diets.

The objective of this study was to investigate the effects of grain sources and forage provision on growth performance, blood metabolites, and feeding behaviors of dairy calves. Sixty 3-d-old Holstein dairy calves (42.2 ± 2.5 kg of body weight) were used in a 2 × 3 factorial arrangement with the factors being grain sources (barley and corn) and forage provision (no forage, alfalfa hay, and corn silage). Individually housed calves were randomly assigned (n = 10 calves per treatment: 5 males and 5 females) to 6 treatments: (1) barley grain (BG) without forage supplement, (2) BG with alfalfa hay (AH) supplementation, (3) BG with corn silage (CS) supplementation, (4) corn grain (CG) without forage supplement, (5) CG with AH supplementation, and (6) CG with CS supplementation. All calves had ad libitum access to water and starter feed throughout the experiment. All calves were weaned on d 49 and remained in the study until d 63. Starter feed intake and average daily gain (ADG) was greater for calves fed barley than those fed corn during the preweaning and overall periods. Calves supplemented with CS had greater final body weight and postweaning as well as overall starter feed intake than AH and non-forage-supplemented calves. During the preweaning and overall periods, feeding of CS was found to increase ADG compared with feeding AH and nonforage diets. However, feed efficiency was not affected by dietary treatments. Calves supplemented with CS spent more time ruminating compared with AH and control groups; nonnutritive oral behaviors were the greatest in non-forage-supplemented calves. Regardless of the grain sources, the rumen pH value was greater for AH calves compared with CS and non-forage-supplemented calves. Blood concentration of BHB was greater for CS-supplemented calves compared with AH and non-forage-supplemented calves. Furthermore, body length and heart girth were greater for calves fed barley compared with those fed corn, and also in forage-supplemented calves compared with non-forage-supplemented calves. These results showed no interactions between grain sources and forage provision on calf performance; however, the inclusion of CS and barley in starter diets could enhance the growth performance of Holstein calves during the transition from liquid to solid feed.

Introducing forage in the young calf diet during the milk-feeding period stimulates rumen development. It was hypothesized that performance in dairy calves would depend on forage provision and starter physical form such that the textured starter (TS) feed with corn silage (CS) supplementation would benefit calf performance. This study evaluates the effects of the physical form of starter diets and CS supplementation on performance, rumen fermentation characteristics, and structural growth of dairy calves. Forty-eight 3-d-old Holstein dairy calves with a mean starting BW of 42.1 kg (SD 2.4) were used in a 2 × 2 factorial arrangement with the factors dietary CS level (0 or 15% on DM basis) and physical form of starter (mashed vs. textured). Individually housed calves were randomly assigned (n = 12 calves per treatment: 6 males and 6 females) to 4 treatments: 1) a mashed starter (MS) feed with no CS (MS-NCS), 2) a MS feed with CS (MS-CS), 3) a TS feed with no CS (TS-NCS), and 4) a TS feed with CS (TS-CS). The calves had ad libitum access to water and starter throughout the study. All calves were weaned on d 56 of age and remained in the study until d 66. The interaction of the physical form of the starter and CS provision was significant (P < 0.01) for the starter intake, with the greatest intake for TS-CS treatment during the preweaning and overall periods. Regardless of the physical form of starter, starter intake, ADG, weaning BW, final BW, ruminal pH, the molar proportion of acetate, and the acetate-to-propionate ratio were greater (P < 0.01) for CS-supplemented calves compared with unsupplemented calves. No interaction (P > 0.05) was detected between the physical form of starter and CS provision with respect to the rumen fermentation parameters and body measurements. Total rumen VFA concentration and the molar proportion of propionate were greater (P < 0.01) in calves fed TS compared with MS-fed calves. In conclusion, independent of the physical form of starter, inclusion of 15% CS in starter diets improves the performance of dairy calves.

Chemical and physical treatments of barley grain increase ruminally resistant starch and can improve the rumen fermentation pattern. The objective of the present study was to evaluate the effects of chemical (addition of citric acid, CA) and physical (grinding to two different particle sizes, PS) treatment of barley grain on performance, rumen fermentation, microbial protein yield in the rumen and selected blood metabolites in growing calves. In all, 28 male Holstein calves (172±5.1 kg initial BW) were used in a complete randomised design with a factorial arrangement of 2 barley grain particle sizes×2 levels of citric acid. The diets were as follows: (i) small PS (average 1200 µm) barley grain soaked in water (no CA addition); (ii) small PS barley grain soaked in a CA solution (adding 20 g CA/kg barley); (iii) large PS (average 2400 µm) barley grain soaked in water (no citric acid addition) and (iv) large PS barley grain soaked in a citric acid solution (adding 20 g CA/kg barley). Barley grain was then incorporated at 35% in a total mixed ration and fed to the calves for 11 weeks. Feeding small PS barley decreased feed intake (P=0.02) and average daily weight gain (P=0.01). The addition of CA to barley grain did not affect intake but increased weight gain (P<0.01) and improved feed to gain ratio (P=0.03). Digestibility of organic matter and NDF tended (P<0.10) to increase, whereas faecal scoring was improved (P=0.03) and the presence of undigested grain particles in faeces was reduced (P<0.01) with CA-treated barley grain. Glucose and urea concentrations were increased (P<0.01) in the blood of calves fed the CA-treated barley grain. Ruminal pH tended (P=0.08) to be decreased with more finely ground barley and was increased when barley grain was treated with CA. Total volatile fatty acid concentrations in the rumen did not differ among treatments (P>0.05). However, the molar proportion of propionate was increased (P=0.03) when barley was more finely ground, and that of acetate was increased (P=0.04) when CA was added to barley grain. The ruminal concentration of ammonia nitrogen was increased (P<0.01) and microbial nitrogen synthesis in the rumen tended to decrease by adding CA to barley. Treating barley grain with citric acid increased fibre digestibility of total mixed rations, attenuated the decrease in ruminal pH, and improved weight gain and feed efficiency in male Holstein growing calves fed a high-cereal diet (550 g cereal grain/kg diet).