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Barbara Kutasy
Campus Keszthely, Department of Plant Physiology and Plant Ecology, Hungarian University of Agriculture and Life Sciences Georgikon, 7 Festetics Str., 8360 Keszthely, Hungary

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Journal article
Published: 10 June 2021 in Sustainability
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Lolium rigidum Gaud. is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally. Acetohydroxyacid synthase (AHAS) resistant populations cause significant problems in cereal production; therefore, monitoring the development of AHAS resistance is widely recommended. Using next-generation sequencing (NGS), a de novo transcriptome sequencing dataset was presented to identify the complete open reading frame (ORF) of AHAS enzyme in L. rigidum and design markers to amplify fragments consisting of all of the eight resistance-conferring amino acid mutation sites. Pro197Thr, Pro197Ala, Pro197Ser, Pro197Gln, and Trp574Leu amino acid substitutions have been observed in samples. Although the Pro197Thr amino acid substitution was already described in SU and IMI resistant populations, this is the first report to reveal that the Pro197Thr in AHAS enzyme confers a high level of resistance (ED50 3.569) to pyroxsulam herbicide (Triazolopyrimidine).

ACS Style

Barbara Kutasy; Zsolt Takács; Judit Kovács; Verëlindë Bogaj; Syafiq Razak; Géza Hegedűs; Kincső Decsi; Kinga Székvári; Eszter Virág. Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass (Lolium Rigidum Gaud.). Sustainability 2021, 13, 6648 .

AMA Style

Barbara Kutasy, Zsolt Takács, Judit Kovács, Verëlindë Bogaj, Syafiq Razak, Géza Hegedűs, Kincső Decsi, Kinga Székvári, Eszter Virág. Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass (Lolium Rigidum Gaud.). Sustainability. 2021; 13 (12):6648.

Chicago/Turabian Style

Barbara Kutasy; Zsolt Takács; Judit Kovács; Verëlindë Bogaj; Syafiq Razak; Géza Hegedűs; Kincső Decsi; Kinga Székvári; Eszter Virág. 2021. "Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass (Lolium Rigidum Gaud.)." Sustainability 13, no. 12: 6648.

Journal article
Published: 10 March 2021 in Diversity
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Background: The spread of herbicide-resistance Ambrosia artemisiifolia threatens not only the production of agricultural crops, but also the composition of weed communities. The reduction of their spread would positively affect the biodiversity and beneficial weed communities in the arable habitats. Detection of resistant populations would help to reduce herbicide exposure which may contribute to the development of sustainable agroecosystems. Methods: This study focuses on the application of target-site resistance (TSR) diagnostic of A. artemisiifolia caused by different herbicides. We used targeted amplicon sequencing (TAS) on Illumina Miseq platform to detect amino acid changes in herbicide target enzymes of resistant and wild-type plants. Results: 16 mutation points of four enzymes targeted by four herbicide groups, such as Photosystem II (PSII), Acetohydroxyacid synthase (AHAS), 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) and protoporphyrinogen IX oxidase (PPO) inhibitors have been identified in common ragweed populations, so far. All the 16 mutation points were analyzed and identified. Out of these, two mutations were detected in resistant biotypes. Conclusions: The applied next-generation sequencing-targeted amplicon sequencing (NGS-TAS) method on A. artemisiifolia resistant and wild-type populations enable TSR detection of large sample numbers in a single reaction. The NGS-TAS provides information about the evolved herbicide resistance that supports the integrated weed control through the reduction of herbicide exposure which may preserve ecological properties in agroecosystems.

ACS Style

Barbara Kutasy; Zoltán Farkas; Balázs Kolics; Kincső Decsi; Géza Hegedűs; Judit Kovács; János Taller; Zoltán Tóth; Nikoletta Kálmán; Gabriella Kazinczi; Eszter Virág. Detection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencing. Diversity 2021, 13, 118 .

AMA Style

Barbara Kutasy, Zoltán Farkas, Balázs Kolics, Kincső Decsi, Géza Hegedűs, Judit Kovács, János Taller, Zoltán Tóth, Nikoletta Kálmán, Gabriella Kazinczi, Eszter Virág. Detection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencing. Diversity. 2021; 13 (3):118.

Chicago/Turabian Style

Barbara Kutasy; Zoltán Farkas; Balázs Kolics; Kincső Decsi; Géza Hegedűs; Judit Kovács; János Taller; Zoltán Tóth; Nikoletta Kálmán; Gabriella Kazinczi; Eszter Virág. 2021. "Detection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencing." Diversity 13, no. 3: 118.

Journal article
Published: 30 July 2020 in Insects
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Applying instrumental insemination in closely related honey bee colonies often leads to frequent lethality of offspring causing colony collapse. This is due to the peculiarities of honey bee reproductive biology, where the complementary sex determination (csd) gene drives sex determination within a haplodiploid system. Diploid drones containing homozygous genotypes are lethal. Tracking of csd alleles using molecular markers prevents this unwanted event in closed breeding programs. Our approach described here is based on high throughput sequencing (HTS) that provides more data than traditional molecular techniques and is capable of analysing sources containing multiple alleles, including diploid individuals as the bee queen. The approach combines HTS technique and clipping wings as a minimally invasive method to detect the complementary sex determiner (csd) alleles directly from honey bee queens. Furthermore, it might also be suitable for screening alleles of honey harvested from hives of a closed breeding facility. Data on alleles of the csd gene from different honey bee subspecies are provided. It might contribute to future databases that could potentially be used to track the origin of honey. With the help of tracking csd alleles, more focused crossings will be possible, which could in turn accelerate honey bee breeding programmes targeting increase tolerance against varroosis as well.

ACS Style

Éva Kolics; Tamás Parrag; Ferenc Házi; Kinga Szepesi; Botond Heltai; Kinga Mátyás; Barbara Kutasy; Eszter Virág; János Taller; László Orbán; Balázs Kolics. An Alternative, High Throughput Method to Identify Csd Alleles of the Honey Bee. Insects 2020, 11, 483 .

AMA Style

Éva Kolics, Tamás Parrag, Ferenc Házi, Kinga Szepesi, Botond Heltai, Kinga Mátyás, Barbara Kutasy, Eszter Virág, János Taller, László Orbán, Balázs Kolics. An Alternative, High Throughput Method to Identify Csd Alleles of the Honey Bee. Insects. 2020; 11 (8):483.

Chicago/Turabian Style

Éva Kolics; Tamás Parrag; Ferenc Házi; Kinga Szepesi; Botond Heltai; Kinga Mátyás; Barbara Kutasy; Eszter Virág; János Taller; László Orbán; Balázs Kolics. 2020. "An Alternative, High Throughput Method to Identify Csd Alleles of the Honey Bee." Insects 11, no. 8: 483.