Gene editing is a versatile technique in biomedicine that promotes fundamental research as well as clinical therapy. The development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) as a genome editing machinery has accelerated the application of gene editing. However, the delivery of CRISPR components often suffers when using conventional transfection methods, such as viral transduction and chemical vectors, due to limited packaging size and inefficiency toward certain cell types. In this review, we discuss physical transfection methods for CRISPR gene editing which can overcome these limitations. We outline different types of physical transfection methods, highlight novel techniques to deliver CRISPR components, and emphasize the role of micro and nanotechnology to improve transfection performance. We present our perspectives on the limitations of current technology and provide insights on the future developments of physical transfection methods.

A boron-doped pyrolyzed Fe–N–C catalyst possesses high stability and the ability to facilitate O₂ dissociation through O₂ side-on adsorption.

A surface acoustic wave (SAW) is a sound wave travelling on the surface of an elastic material. SAW offers a robust control of the acoustic energy leading to an unparalleled versatility. As an actuator, SAW can exert acoustic forces on particles and fluids thus enabling dexterous micro/nanoscale manipulations. As a sensor, SAW has a unique sensing capability upon changes in the environment. On-chip SAW technology, in which SAW is integrated with modern lab-on-a-chip (LOC), has drawn a lot of attention in recent years and found various exciting applications in micro/nanosystems. In particular, its well-known biocompatibility provides on-chip SAW technology as an exceptional platform for biomaterials research at the small-scale. In this minireview, we highlighted recent advances of on-chip SAW technology for biomaterials manipulation and characterization with a focus on cell-based (e.g. single-cell and multicellular) biomaterials. We also discussed and shared our perspective on future directions for this emerging research field.

Manipulation of micro/nano particles has been well studied and demonstrated by optical, electromagnetic, and acoustic approaches, or their combinations. Manipulation of internal structure of droplet/particle is rarely explored and remains challenging due to its complicated nature. Here we demonstrated the manipulation of internal structure of disk-in-sphere endoskeletal droplets using acoustic wave. We developed a model to investigate the physical mechanisms behind this interesting phenomenon. Theoretical analysis of the acoustic interactions indicated that these assembly dynamics arise from a balance of the primary and secondary radiation forces. Additionally, the disk orientation was found to change with acoustic driving frequency, which allowed on-demand, reversible adjustment of the disk orientations with respect to the substrate. This dynamic behavior leads to unique reversible arrangements of the endoskeletal droplets and their internal architecture, which may provide an avenue for directed assembly of novel hierarchical colloidal architectures and intracellular organelles or intra-organoid structures.