Freshwater ecosystems are responsible for an important part of the methane (CH₄) emissions which are likely to change with global warming. This study aims to evaluate temperature-induced (from 5 to 20 °C) changes on microbial community structure and methanogenic pathways in five sub-Antarctic lake sediments from Magallanes strait to Cape Horn, Chile. We combined in situ CH₄ flux measurements, CH₄ production rates (MPRs), gene abundance quantification and microbial community structure analysis (metabarcoding of the 16S rRNA gene). Under unamended conditions, a temperature increase of 5 °C doubled MPR while microbial community structure was not affected. Stimulation of methanogenesis by methanogenic precursors as acetate and H₂/CO₂, resulted in an increase of MPRs up to 127-fold and 19-fold, respectively, as well as an enrichment of mcrA-carriers strikingly stronger under acetate amendment. At low temperatures, H₂/CO₂-derived MPRs were considerably lower (down to 160-fold lower) than the acetate-derived MPRs, but the contribution of hydrogenotrophic methanogenesis increased with temperature. Temperature dependence of MPRs was significantly higher in incubations spiked with H₂/CO₂ (c. 1.9 eV) compared to incubations spiked with acetate or unamended (c. 0.8 eV). Temperature was not found to shape the total microbial community structure, that rather exhibited a site-specific variability among the studied lakes. However, the methanogenic archaeal community structure was driven by amended methanogenic precursors with a dominance of Methanobacterium in H₂/CO₂-based incubations and Methanosarcina in acetate-based incubations. We also suggested the importance of acetogenic H₂-production outcompeting hydrogenotrohic methanogenesis especially at low temperatures, further supported by homoacetogen proportion in the microcosm communities. The combination of in situ-, and laboratory-based measurements and molecular approaches indicates that the hydrogenotrophic pathway may become more important with increasing temperatures than the acetoclastic pathway. In a continuously warming environment driven by climate change, such issues are crucial and may receive more attention.

This study describes, for the first time, the water chemistry and microbial diversity in Dziani Dzaha, a tropical crater lake located on Mayotte Island (Comoros archipelago, Western Indian Ocean). The lake water had a high level of dissolved matter and high alkalinity (10.6–14.5 g L^-1 eq. CO₃^2-_, i.e. 160–220 mM compare to around 2–2.5 in seawater), with salinity up to 52 psu, 1.5 higher than seawater. Hierarchical clustering discriminated Dziani Dzaha water from other alkaline, saline lakes, highlighting its thalassohaline nature. The phytoplankton biomass was very high, with a total chlorophyll a concentration of 524 to 875 μg chl a L^-1 depending on the survey, homogeneously distributed from surface to bottom (4 m). Throughout the whole water column the photosynthetic biomass was dominated (>97% of total biovolume) by the filamentous cyanobacteria Arthrospira sp. with a straight morphotype. In situ daily photosynthetic oxygen production ranged from 17.3 to 22.2 g O₂ m^-2 d^-1, consistent with experimental production / irradiance measurements and modeling. Heterotrophic bacterioplankton was extremely abundant, with cell densities up to 1.5 10⁸ cells mL^-1 in the whole water column. Isolation and culture of 59 Eubacteria strains revealed the prevalence of alkaliphilic and halophilic organisms together with taxa unknown to date, based on 16S rRNA gene analysis. A single cloning-sequencing approach using archaeal 16S rDNA gene primers unveiled the presence of diverse extremophilic Euryarchaeota. The water chemistry of Dziani Dzaha Lake supports the hypothesis that it was derived from seawater and strongly modified by geological conditions and microbial activities that increased the alkalinity. Dziani Dzaha has a unique consortium of cyanobacteria, phytoplankton, heterotrophic Eubacteria and Archaea, with very few unicellular protozoa, that will deserve further deep analysis to unravel its uncommon diversity. A single taxon, belonging to the genus Arthrospira, was found responsible for almost all photosynthetic primary production.

The Anthropocene is an uneven phenomenon. Accelerated shifts in the functioning of the Earth System are mainly driven by the production and consumption of wealthy economies. Social, environmental and health costs of such industrialization, however, bear on low‐income communities inhabiting severely degraded territories by polluting activities (i.e., sacrifice zones). How global, national and local socio‐economic and governance processes have interacted in perpetuating socio‐environmental inequalities in these territories has been rarely explored. Here, we develop an historical quantitative approach integrating a novel chemostratigraphic record, data on policy making, and socio‐economic trends to evaluate the feedback relationship between environmental injustice and Anthropocene in sacrifice zones. We specifically outline a case study for the Puchuncaví valley ‐one of the most emblematic sacrifice zones from Chile‐. We verify an ever‐growing burden of heavy metals and metalloids over the past five decades paced by the staggering expansion of local industrial activities, which has ultimately been spurred by national and transnational market forces. Local poverty levels have declined concomitantly, but this path toward social equality is marginal as costs of pollution have grown through time. Indeed, national and international pollution control actions appear insufficient in mitigating the cumulative impact brought by highly toxic elements. Thus, our sub‐decadal reconstruction for pollution trends over the past 136 years from a sediment record, emerges as a science‐based tool for informing the discussion on Anthropocene governance. Furthermore, it helps to advance in the assessment of environmental inequality in societal models that prioritize economic growth to the detriment of socio‐environmental security.

The Aconcagua river basin (Chile, 32 °S) has suffered the effects of the megadrought over the last decade. The severe snowfall deficiency drastically modified the water supply to the catchment headwaters. Despite the recognized snowmelt contribution to the basin, an unknown streamflow buffering effect is produced by glacial, periglacial and groundwater inputs, especially in dry periods. Hence, each type of water source was characterized and quantified for each season, through the combination of stable isotope and ionic analyses as natural water tracers. The δ¹⁸O and electric conductivity were identified as the key parameters for the differentiation of each water source. The use of these parameters in the stable isotope mixing “simmr” model revealed that snowmelt input accounted 52% in spring and only 22–36% during the rest of the year in the headwaters. While glacial supply contributed up to 34%, both groundwater and periglacial exhibited a remarkable contribution around 20% with some seasonal variations. Downstream, glacial contribution averaged 15–20%, groundwater seasonally increased up to 46%, and periglacial input was surprisingly high (i.e., 14–21%). The different water sources contribution quantification over time for the Aconcagua River reported in this work provides key information for water security in this territory.