Xylella fastidiosa subsp. pauca (Xfp) is one of the most dangerous plant pathogens in the world. Identified in 2013 in olive trees in south–eastern Italy, it is spreading to the Mediterranean countries. The bacterium is transmitted by insects that feed on sap, and causes rapid wilting in olive trees. The paper explores the use of Unmanned Aerial Vehicle (UAV) in combination with a multispectral radiometer for early detection of infection. The study was carried out in three olive groves in the Apulia region (Italy) and involved four drone flights from 2017 to 2019. To classify Xfp severity level in olive trees at an early stage, a combined method of geostatistics and discriminant analysis was implemented. The results of cross-validation for the non-parametric classification method were of overall accuracy = 0.69, mean error rate = 0.31, and for the early detection class of accuracy 0.77 and misclassification probability 0.23. The results are promising and encourage the application of UAV technology for the early detection of Xfp infection.

Xylella fastidiosa is a bacterial pathogen affecting many plant species worldwide. Recently, the subspecies pauca (Xfp) has been reported as the causal agent of a devastating disease on olive trees in the Salento area (Apulia region, southeastern Italy), where centenarian and millenarian plants constitute a great agronomic, economic, and landscape trait, as well as an important cultural heritage. It is, therefore, important to develop diagnostic tools able to detect the disease early, even when infected plants are still asymptomatic, to reduce the infection risk for the surrounding plants. The reference analysis is the quantitative real time-Polymerase-Chain-Reaction (qPCR) of the bacterial DNA. The aim of this work was to assess whether the analysis of hyperspectral data, using different statistical methods, was able to select with sufficient accuracy, which plants to analyze with PCR, to save time and economic resources. The study area was selected in the Municipality of Oria (Brindisi). Partial Least Square Regression (PLSR) and Canonical Discriminant Analysis (CDA) indicated that the most important bands were those related to the chlorophyll function, water, lignin content, as can also be seen from the wilting symptoms in Xfp-infected plants. The confusion matrix of CDA showed an overall accuracy of 0.67, but with a better capability to discriminate the infected plants. Finally, an unsupervised classification, using only spectral data, was able to discriminate the infected plants at a very early stage of infection. Then, in phase of testing qPCR should be performed only on the plants predicted as infected from hyperspectral data, thus, saving time and financial resources.

In some regions of Italy, low intensity farming systems, together with variable climate condition, have lowered soil organic carbon (SOC) content and soil quality attributes. This work aims to investigate on some aspects of: (1) TOC prediction using Vis-NIR reflectance spectroscopy in combination with partial least squares regression (PLSR); (2) the most appropriate pre-processing techniques of Vis-NIR absorbance spectra; (3) the composition of organic carbon using variable importance of prediction (VIP). The study area was an olive grove, located at Montecorvino Rovella (Salerno, southwestern Italy), characterized by a calcaric soil (Leptic Calcisols) and (Luvic Phaeozem), with a low content of TOC (mean 2.03 g kg⁻¹), caused by a low intensity farming. Results of univariate PLSR analyses showed a good agreement between measured and predicted values both for TOC (R²: 0.66) and total carbonate content (R²: 0.93), when pH, electrical conductivity (EC) and absorbance spectra were used as predictors. The best results were obtained using as pre-treatments of the spectral data: 1) standard normal variate (SNV); 2) Savitzky-Golay algorithm; 3) first derivative. Variable Importance for Prediction (VIP) statistics showed to be a good tool to gain insights in TOC composition also when its content is low and influenced by carbonate.

Summary: SPAD combined with hyperspectral sensors can be an alternative approach to traditional laboratory methods for determining the physiological status of trees. The aim of this work was to assess whether the effectiveness of SPAD predictive models using hyperspectral data might be influenced by where the measurements were carried out. Leaves of apricot trees of two varieties (Farbaly and Farlis) were analysed with SPAD and spectroradiometer, and the data were organized in two different ways: (i) overall dataset (OD), collecting total measurements of trees in each variety; (ii) subset of overall datasets (SOD), collecting the measurements performed on the cardinal points of trees in each variety. Prediction models were built using as regressors: (i) spectral data transformed with Continuum Removal (CR) methodology (CR indices); (ii) vegetation indices (VI) linked to chlorophyll and nitrogen content; (iii) reflectance values associated with chlorophyll content and to wavelengths ranges where (CR) methodology was applied; (iv) reflectance values of full spectrum. The best performances belonged to models using wider ranges of spectrum both in ODs and in SODs. The north cardinal point showed prediction models with the best performances in both varieties. No VI and CR indices showed reliable models. All the reliable prediction models were associated with compounds involved in physiological state and metabolism of leaves in apricot tree.

Remote sensing technologies continue to expand their role in environmental monitoring, providing invaluable advances in soil assessing and mapping. This study aimed to prove the need to apply spatial statistical models for processing data in remote sensing (RS), which appears to be an important source of spatial data at multiple scales. A crucial problem facing us is the fusion of multi-source spatial data of different natures and characteristics, among which there is the support size of measurement that unfortunately is little considered in RS. A data fusion approach of both sample (point) and grid (areal) data is proposed that explicitly takes into account spatial correlation and change of support in both increasing support (upscaling) and decreasing support (downscaling). The techniques of block cokriging and kriging downscaling were employed for the implementation of such an approach, respectively. The method is applied to soil sample data, jointly analysed with hyperspectral data measured in the laboratory, UAV, and satellite data (Planet and Sentinel 2) of an olive grove after filtering soil pixels. Each data type had its own support that was transformed to the same support as the soil sample data so that the data fusion approach could be applied. To demonstrate the statistical, as well as practical, effectiveness of such a method, it was compared by a cross-validation test with a univariate approach for predicting each soil property. The positive results obtained should stimulate advanced statistical techniques to be applied more and more widely to RS data.

Traditional soil characterization methods are time consuming, laborious and invasive and do not allow for long-term repeatability of measurements. The overall aim of this paper was to assess and model spatial variability of the soil in an olive grove in south Italy by using data from two sensors of different types: a multi-spectral on-board drone radiometer and a hyperspectral visible-near infrared-shortwave infrared (VIS-NIR-SWIR) reflectance radiometer, as well as sample data, to arrive at a delineation of homogeneous areas. The hyperspectral data were processed using Continuum Removal (CR) methodology to obtain information about the content and composition of clay. Differently, the multispectral data were firstly upscaled to the support of soil data using geostatistics and taking into account the change of support. Secondly, the data acquired with the two different sensors were integrated with soil granulometric properties by using two multivariate geostatistical techniques: multi-collocated cokriging to achieve a more exhaustive and finer-scale soil characterization, and multi-collocated factor cokriging to extract synthetic scale-dependent indices (regionalized factors) for the delineation of soil in homogeneous zones. This paper shows the impact of change of support on the uncertainty of soil prediction that can have a significant effect on decision making in Precision Agriculture. Moreover, four regionalized factors at two different scales (two for each scale) were retained and mapped. Each factor provided a different delineation of the field with areas characterized by different granulometries and clay compositions. The applied method is sufficiently flexible and could be applied to any number and type of sensors.