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Opuntia sp. contain antioxidant phytochemicals resistant to ROS damage, whose excess negatively affect fertilization. We investigate the activity of fruit extracts of O. dillenii and O. ficus indica (cv red and yellow) on sperm quality and cryopreservation. In the first experiment, we exposed the samples to extracts (50 µl) for 1 hour to then evaluate semen parameters (vitality, motility, acrosome reaction, DNA fragmentation and oxidative stress). The results showed a significant increase in the motility (86%±0.19 for OFI cv yellow, 82%±0.15 for OFI cv red and 90%±0.08 for O. dillenii) compared to the control (80%±0.17). Moreover, we noted a reduction of DNA fragmentation on treated (3%±0.03 in OFI cv yellow, 7%±0.09 in OFI cv red and 5%±0.07 in O. dillenii) than the control (40%±0.14). Furthermore, the oxidative stress was reduced after exposure to solutions (3.15mV in the control and 2.94mV in the treated). In the second experiment, 50 µl of solutions were added to the Freezing medium. After thawing, we observed an improvement in vitality and the number of intact acrosomes. Our results suggest that Opuntia sp. fruit extracts improve sperm quality, both before and after cryopreservation, optimizing the potential of fertilization of sperm cells.
Martina Contino; Elena Maria Scalisi; Roberta Pecoraro; Chiara Failla; Sara Ignoto; Lucia Buonfrate; Antonio Salvaggio; Pietro Calderaro; Claudia Genovese; Salvatore Antonino Raccuia; Paola Asero; Giovanna Caruso; Giovanni Bracchitta; Maria Violetta Brundo. Antioxidant Activity of Opuntia SP. Fruit Extracts on Human Sperm Quality and Cryopreservation Cycle. 2021, 1 .
AMA StyleMartina Contino, Elena Maria Scalisi, Roberta Pecoraro, Chiara Failla, Sara Ignoto, Lucia Buonfrate, Antonio Salvaggio, Pietro Calderaro, Claudia Genovese, Salvatore Antonino Raccuia, Paola Asero, Giovanna Caruso, Giovanni Bracchitta, Maria Violetta Brundo. Antioxidant Activity of Opuntia SP. Fruit Extracts on Human Sperm Quality and Cryopreservation Cycle. . 2021; ():1.
Chicago/Turabian StyleMartina Contino; Elena Maria Scalisi; Roberta Pecoraro; Chiara Failla; Sara Ignoto; Lucia Buonfrate; Antonio Salvaggio; Pietro Calderaro; Claudia Genovese; Salvatore Antonino Raccuia; Paola Asero; Giovanna Caruso; Giovanni Bracchitta; Maria Violetta Brundo. 2021. "Antioxidant Activity of Opuntia SP. Fruit Extracts on Human Sperm Quality and Cryopreservation Cycle." , no. : 1.
The focus of this work was to investigate the toxicity of different metal nanoparticles (gold nanoparticles [AuNPs], silver nanoparticles [AgNPs], titanium dioxide nanoparticles [TiO2NPs]) on brine shrimp Artemia salina. We investigated if nanoparticles could have an influence on hatching of cysts and on mortality of larvae. Larvae (also called nauplii) and cysts were exposed to NPs for 24 hr in artificial seawater on microplates. At the end of the test, we assessed the endpoint (immobility/death) for the larvae by a stereomicroscope. Nauplii that appeared completely motionless, were counted as dead, and the percentages of mortality were calculated for each treatment. Instead for the cysts, the percentages of not‐hatched nauplii for each concentration considered were calculated by counting the number of whole cysts. Currently, nanoparticles toxicity has been investigated in several research; in our study we highlighted the nontoxicity of TiO2NPs on A. salina nauplii as shown by low percentages of immobilization and on cysts because TiO2NPs do not affect their hatching. Despite AuNPs exerted toxic effects on hatching, they did not affect larvae development as well as TiO2NPs. Otherwise, AgNPs induced mortality of the larvae and inhibited cysts hatching.
Roberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Giuseppe Fragalà; Sara Ignoto; Antonio Salvaggio; Massimo Zimbone; Giuliana Impellizzeri; Maria Violetta Brundo. Artemia salina : A microcrustacean to assess engineered nanoparticles toxicity. Microscopy Research and Technique 2020, 84, 531 -536.
AMA StyleRoberta Pecoraro, Elena Maria Scalisi, Giuseppina Messina, Giuseppe Fragalà, Sara Ignoto, Antonio Salvaggio, Massimo Zimbone, Giuliana Impellizzeri, Maria Violetta Brundo. Artemia salina : A microcrustacean to assess engineered nanoparticles toxicity. Microscopy Research and Technique. 2020; 84 (3):531-536.
Chicago/Turabian StyleRoberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Giuseppe Fragalà; Sara Ignoto; Antonio Salvaggio; Massimo Zimbone; Giuliana Impellizzeri; Maria Violetta Brundo. 2020. "Artemia salina : A microcrustacean to assess engineered nanoparticles toxicity." Microscopy Research and Technique 84, no. 3: 531-536.
Pollution and other types of environmental stress do not spare marine environments, especially those affected by high industrial pressure. Fish, especially coastal species, are used for monitoring the marine environment because they are particularly efficient as bioindicators thanks to their ability to bioaccumulate and biomagnify along the trophic chain. The aim of this research is to evaluate the bioaccumulation and the indirect bioindication ability of the European Hake, Merluccius merluccius (Linnaeus, 1758), one of the most important commercial fish species of the Mediterranean Sea. Morphological and histological alterations of the main target organs, such as liver and gills, have been investigated and the results showed a steatosis in the hepatic tissue. The accumulation of heavy metals has been analyzed by inductively coupled plasma mass spectrometry and for several metals it was showed a different concentration in the two sexes. Moreover, the expression of metallothioneins 1 and Heat Shock Protein 70 has been assessed by immunohistochemistry and did not show high level of expression. We underline the importance of contamination evaluation in commercial fish species and the utilization of the ichthyofauna as bioindicator of environmental quality.
Antonio Salvaggio; Roberta Pecoraro; Chiara Copat; Margherita Ferrante; Alfina Grasso; Elena Maria Scalisi; Sara Ignoto; Vincenza Serena Bonaccorsi; Giuseppina Messina; Bianca Maria Lombardo; Francesco Tiralongo; Maria Violetta Brundo. Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment. Journal of Marine Science and Engineering 2020, 8, 712 .
AMA StyleAntonio Salvaggio, Roberta Pecoraro, Chiara Copat, Margherita Ferrante, Alfina Grasso, Elena Maria Scalisi, Sara Ignoto, Vincenza Serena Bonaccorsi, Giuseppina Messina, Bianca Maria Lombardo, Francesco Tiralongo, Maria Violetta Brundo. Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment. Journal of Marine Science and Engineering. 2020; 8 (9):712.
Chicago/Turabian StyleAntonio Salvaggio; Roberta Pecoraro; Chiara Copat; Margherita Ferrante; Alfina Grasso; Elena Maria Scalisi; Sara Ignoto; Vincenza Serena Bonaccorsi; Giuseppina Messina; Bianca Maria Lombardo; Francesco Tiralongo; Maria Violetta Brundo. 2020. "Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment." Journal of Marine Science and Engineering 8, no. 9: 712.
Recently two-dimensional nanomaterials, such as graphene and molybdenum disulfide (MoS2), have received much attention as adsorbent materials for the effective removal of organic contaminants. MoS2 is attracting attention, not only for its chemical-physical properties, but also for its wide availability in nature as a constituent of molybdenite. The aim of this investigation was to assess the effects of different MoS2 concentrations (5 × 10−1, 5 × 10−2 and 5 × 10−3 mg/ml) on the embryonated eggs of Gallus gallus domesticus, according to Beck method. We evaluated the toxic effect of the MoS2 powder purchased at Sigma-Aldrich indicated as “received” and MoS2 powder treated via mechanical milling indicated as “ball mille”. Subsequently, the embryos were sacrificed at different times of embryonic development (11th, 15th and 19th day after incubation) in order to evaluate their embryotoxic and teratogenic effects. The alterations of the embryonic development were studied by morphological and immunohistochemical analysis of the tissues. The results obtained have shown the toxicity of both powders of MoS2 with a high percentage of deaths and growth delays. Moreover, the immunohistochemical analysis performed on several tissue sections showed a strong positivity to the anti-metallothionein1 antibody only for the erythrocytes.
Elena Maria Scalisi; Antonio Salvaggio; Francesco Antoci; Antonio Messina; Roberta Pecoraro; Maria Cantarella; Giuliana Gorrasi; Giuliana Impellizzeri; Maria Violetta Brundo. Toxicity assessment of two-dimensional nanomaterials molybdenum disulfide in Gallus gallus domesticus. Ecotoxicology and Environmental Safety 2020, 200, 110772 .
AMA StyleElena Maria Scalisi, Antonio Salvaggio, Francesco Antoci, Antonio Messina, Roberta Pecoraro, Maria Cantarella, Giuliana Gorrasi, Giuliana Impellizzeri, Maria Violetta Brundo. Toxicity assessment of two-dimensional nanomaterials molybdenum disulfide in Gallus gallus domesticus. Ecotoxicology and Environmental Safety. 2020; 200 ():110772.
Chicago/Turabian StyleElena Maria Scalisi; Antonio Salvaggio; Francesco Antoci; Antonio Messina; Roberta Pecoraro; Maria Cantarella; Giuliana Gorrasi; Giuliana Impellizzeri; Maria Violetta Brundo. 2020. "Toxicity assessment of two-dimensional nanomaterials molybdenum disulfide in Gallus gallus domesticus." Ecotoxicology and Environmental Safety 200, no. : 110772.
Three different Advanced Oxidation Processes (AOPs) have been investigated for the degradation of the imidacloprid pesticide in water: photocatalysis, Fenton and photo-Fenton reactions. For these tests, we have compared the performance of two types of CeO2, employed as a non-conventional photocatalyst/Fenton-like material. The first one has been prepared by chemical precipitation with KOH, while the second one has been obtained by exposing the as-synthetized CeO2 to solar irradiation in H2 stream. This latter treatment led to obtain a more defective CeO2 (coded as “grey CeO2”) with the formation of Ce3+ sites on the surface of CeO2, as determined by Raman and X-ray Photoelectron Spectroscopy (XPS) characterizations. This peculiar feature has been demonstrated as beneficial for the solar photo–Fenton reaction, with the best performance exhibited by the grey CeO2. On the contrary, the bare CeO2 showed a photocatalytic activity higher with respect to the grey CeO2, due to the higher exposed surface area and the lower band-gap. The easy synthetic procedures of CeO2 reported here, allows to tune and modify the physico-chemical properties of CeO2, allowing a choice of different CeO2 samples on the basis of the specific AOPs for water remediation. Furthermore, neither of the samples have shown any critical toxicity.
Roberto Fiorenza; Stefano Andrea Balsamo; Luisa D’Urso; Salvatore Sciré; Maria Violetta Brundo; Roberta Pecoraro; Elena Maria Scalisi; Vittorio Privitera; Giuliana Impellizzeri. CeO2 for Water Remediation: Comparison of Various Advanced Oxidation Processes. Catalysts 2020, 10, 446 .
AMA StyleRoberto Fiorenza, Stefano Andrea Balsamo, Luisa D’Urso, Salvatore Sciré, Maria Violetta Brundo, Roberta Pecoraro, Elena Maria Scalisi, Vittorio Privitera, Giuliana Impellizzeri. CeO2 for Water Remediation: Comparison of Various Advanced Oxidation Processes. Catalysts. 2020; 10 (4):446.
Chicago/Turabian StyleRoberto Fiorenza; Stefano Andrea Balsamo; Luisa D’Urso; Salvatore Sciré; Maria Violetta Brundo; Roberta Pecoraro; Elena Maria Scalisi; Vittorio Privitera; Giuliana Impellizzeri. 2020. "CeO2 for Water Remediation: Comparison of Various Advanced Oxidation Processes." Catalysts 10, no. 4: 446.
The aim of this study was to evaluate the toxicity of titanium dioxide nanoparticles (TiO2NPs) by short‐term toxicity tests in Apis mellifera, considered an excellent bioindicator organism mainly due to its sensitivity. Bees have been exposed to several concentrations of TiO2NPs (1 × 10−3, 1 × 10−4, 1 × 10−5, 1 × 10−6 mg/10 ml) for 10 days. Morphostructural and histological assays were done on gut and honey sac. The research of exposure biomarkers like metallothioneins 1 (MT1) and Heat Shock Protein 70 (HSP70) was performed to verify if a detoxification mechanism has been activated in the exposed animals. No histological alteration on the epithelium of the gut and honey sac were observed in exposed samples. A significant positivity for anti‐MT1 antibody was observed only in the honey sac cells. A weak positivity for HSP70 was observed in both structures analyzed. In several studies have shown the non‐toxicity of TiO2NPs on other model organisms, in our study, titanium dioxide nanoparticles was proven to be highly toxic at the highest concentration tested (100% of lethality to 1 × 10−3 mg/10 ml) and moderately toxic at lower concentrations. Honey bees proved to be excellent models for study of NPs toxicity and for monitoring environment.
Giulia Ferrara; Antonio Salvaggio; Roberta Pecoraro; Elena Maria Scalisi; Andrea Maria Presti; Giuliana Impellizzeri; Maria Violetta Brundo. Toxicity assessment of nano‐TiO 2 in Apis mellifera L., 1758: histological and immunohistochemical assays. Microscopy Research and Technique 2019, 83, 332 -337.
AMA StyleGiulia Ferrara, Antonio Salvaggio, Roberta Pecoraro, Elena Maria Scalisi, Andrea Maria Presti, Giuliana Impellizzeri, Maria Violetta Brundo. Toxicity assessment of nano‐TiO 2 in Apis mellifera L., 1758: histological and immunohistochemical assays. Microscopy Research and Technique. 2019; 83 (4):332-337.
Chicago/Turabian StyleGiulia Ferrara; Antonio Salvaggio; Roberta Pecoraro; Elena Maria Scalisi; Andrea Maria Presti; Giuliana Impellizzeri; Maria Violetta Brundo. 2019. "Toxicity assessment of nano‐TiO 2 in Apis mellifera L., 1758: histological and immunohistochemical assays." Microscopy Research and Technique 83, no. 4: 332-337.
Invertebrates are precious organisms in order to study environmental pollution. In particular, they appear to be suitable as a bioindicator species for pioneer ecotoxicity studies on new xenobiotics such as nanoparticles. In fact, they are able to absorb nanomaterials scattered in the environment in different ways and it's known the compartmentalization of nano‐sized contaminants in selected tissues and intracellular organelles. Titanium dioxide represents the most used nanoparticulate, destined to become probably ubiquitous in the environment. Recently, some research has been published on the toxic potential of nano‐TiO2 in several animal species. Among all invertebrates, Oniscidean Isopods are the only taxon of Crustaceans that has become completely terrestrial, known as excellent bioindicators and bioaccumulators. They have a digestive gland, the hepatopancreas, which is the location of election for the accumulation of pollutants. For this reason, they are considered efficient animal models to ecological studies. For this study, we collected Armadillo officinalis from Natural Oriented Reserve of “Vendicari” (Sicily, Italy), to evaluate the toxicity of titanium dioxide (TiO2) on their hepatopancreas, after a short period of exposure. We conducted morphostructural and immunohistochemistry assays. The results suggested a great capacity of the species of bioaccumulation of nanoparticles in the hepatopancreas, where a strong positivity to the metallothioneins was highlighted. Our study confirms that Oniscidean Isopods, in particular Armadillo officinalis, proved to be an appropriate indicator of pollution in terrestrial ecosystems from nanoparticles.
Alessia Flaccavento; Roberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Antonio Salvaggio; Giuliana Impellizzeri; Bianca Maria Lombardo; Maria Violetta Brundo. Morphostructural and immunohistochemical study for evaluation of nano‐TiO 2 toxicity in Armadillo officinalis Duméril, 1816 (Crustacea, Isopoda, Oniscidea). Microscopy Research and Technique 2019, 83, 297 -303.
AMA StyleAlessia Flaccavento, Roberta Pecoraro, Elena Maria Scalisi, Giuseppina Messina, Antonio Salvaggio, Giuliana Impellizzeri, Bianca Maria Lombardo, Maria Violetta Brundo. Morphostructural and immunohistochemical study for evaluation of nano‐TiO 2 toxicity in Armadillo officinalis Duméril, 1816 (Crustacea, Isopoda, Oniscidea). Microscopy Research and Technique. 2019; 83 (3):297-303.
Chicago/Turabian StyleAlessia Flaccavento; Roberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Antonio Salvaggio; Giuliana Impellizzeri; Bianca Maria Lombardo; Maria Violetta Brundo. 2019. "Morphostructural and immunohistochemical study for evaluation of nano‐TiO 2 toxicity in Armadillo officinalis Duméril, 1816 (Crustacea, Isopoda, Oniscidea)." Microscopy Research and Technique 83, no. 3: 297-303.
In recent years, the Mediterranean Sea has become an accumulation zone for waste generated by the 22 countries bordering its shores. Although the effects of plastic litter on the marine environment and on organisms have recently been studied in other areas, further information is needed for the Mediterranean Sea and, in particular, about plastics additives inputs and interactions with the biota and the trophic network, such as phthalates and bisphenol A. Plastic material production, use and disposal contribute also to the release of heavy metals into the environment, such as mercury (Hg), often used during the production of chlorine, the primary ingredient in PVC, lead (Pb) and cadmium (Cd), which are used as stabilizers in PVC and leach out of products during use and disposal. Our research aims to evaluate phthalates, bisphenol A and heavy metals contamination in Lepidopus caudatus (Pisces, Trichiuridae), which could be considered as a potential sentinel species. For the evaluation of toxicological effects, we evaluated the expression of vitellogenin and metallothioneins 1. In all samples analyzed, we have not found microplastics in the gastrointestinal tract but chemical analysis revealed the presence of high content of phthalates, and in particular high quantities of DIDP, DEHP, bis-benzylester phthalate, bis-butyl ester phthalate and mono-N-butyl ester phthalate in different organs. Instead, trace elements detected in tissue revealed a trend of concentrations generally higher in liver and intestine than gill and muscle tissues. Immunohistochemical analysis for anti-metallothionein 1 antibody showed a strong positivity of liver cells, both in females and males. Analysis for the anti-vitellogenin antibody showed in females a strong positivity both in the liver cells and in the gonads, in male specimens was found to be always negative except for a specimen, in which it was highlighted a positivity in some areas of the liver and of the gonad.
Antonio Salvaggio; Francesco Tiralongo; Evangelia Krasakopoulou; Dimitra Marmara; Ioannis Giovos; Rosalia Crupi; Giuseppina Messina; Bianca Maria Lombardo; Alessandra Marzullo; Roberta Pecoraro; Elena Maria Scalisi; Chiara Copat; Pietro Zuccarello; Margherita Ferrante; Maria Violetta Brundo. Biomarkers of Exposure to Chemical Contamination in the Commercial Fish Species Lepidopus caudatus (Euphrasen, 1788): A Particular Focus on Plastic Additives. Frontiers in Physiology 2019, 10, 905 .
AMA StyleAntonio Salvaggio, Francesco Tiralongo, Evangelia Krasakopoulou, Dimitra Marmara, Ioannis Giovos, Rosalia Crupi, Giuseppina Messina, Bianca Maria Lombardo, Alessandra Marzullo, Roberta Pecoraro, Elena Maria Scalisi, Chiara Copat, Pietro Zuccarello, Margherita Ferrante, Maria Violetta Brundo. Biomarkers of Exposure to Chemical Contamination in the Commercial Fish Species Lepidopus caudatus (Euphrasen, 1788): A Particular Focus on Plastic Additives. Frontiers in Physiology. 2019; 10 ():905.
Chicago/Turabian StyleAntonio Salvaggio; Francesco Tiralongo; Evangelia Krasakopoulou; Dimitra Marmara; Ioannis Giovos; Rosalia Crupi; Giuseppina Messina; Bianca Maria Lombardo; Alessandra Marzullo; Roberta Pecoraro; Elena Maria Scalisi; Chiara Copat; Pietro Zuccarello; Margherita Ferrante; Maria Violetta Brundo. 2019. "Biomarkers of Exposure to Chemical Contamination in the Commercial Fish Species Lepidopus caudatus (Euphrasen, 1788): A Particular Focus on Plastic Additives." Frontiers in Physiology 10, no. : 905.
Although in recent years there has been intensification in the use of nanoparticles (NPs) for the production of many commercial products, few studies have been carried out to assess the risks associated with its use. Among the most used NPs, silver nanoparticles (AgNPs) have a preponderant position as they have various applications. In this study, 40 adult zebrafish were exposed to increasing concentrations of AgNPs (8, 45, and 70 μg/L) for 30 days to evaluate the effects on eyes after chronic exposure to AgNPs with an average diameter of 50 nm. From the morphological and ultrastructural analysis performed, no alteration or lesions of the corneal epithelium were detected.
Roberta Pecoraro; Antonio Salvaggio; Elena Maria Scalisi; Carmelo Iaria; Giovanni Lanteri; Chiara Copat; Margherita Ferrante; Giuseppe Fragalà; Massimo Zimbone; Giuliana Impellizzeri; Maria V. Brundo. Evaluation of the effects of silver nanoparticles on Danio rerio cornea: Morphological and ultrastructural analysis. Microscopy Research and Technique 2019, 82, 1297 -1301.
AMA StyleRoberta Pecoraro, Antonio Salvaggio, Elena Maria Scalisi, Carmelo Iaria, Giovanni Lanteri, Chiara Copat, Margherita Ferrante, Giuseppe Fragalà, Massimo Zimbone, Giuliana Impellizzeri, Maria V. Brundo. Evaluation of the effects of silver nanoparticles on Danio rerio cornea: Morphological and ultrastructural analysis. Microscopy Research and Technique. 2019; 82 (8):1297-1301.
Chicago/Turabian StyleRoberta Pecoraro; Antonio Salvaggio; Elena Maria Scalisi; Carmelo Iaria; Giovanni Lanteri; Chiara Copat; Margherita Ferrante; Giuseppe Fragalà; Massimo Zimbone; Giuliana Impellizzeri; Maria V. Brundo. 2019. "Evaluation of the effects of silver nanoparticles on Danio rerio cornea: Morphological and ultrastructural analysis." Microscopy Research and Technique 82, no. 8: 1297-1301.
In the last years, the increase of anthropogenic activities has led to a progressive pollution of ecosystems. Oniscidean isopods are a group of terrestrial animals known as excellent bio‐indicators and bio‐accumulators, because of their capacity to accumulate contaminants and to implement appropriate strategies of detoxification. For this study, we collected Porcellionides pruinosus from two different sites in the Corinth region (Greece), a polluted site and a control site, to assess the heavy metal pollution status. We conducted an inductively coupled plasma mass spectrometry (ICP‐MS) analysis for the detection of trace elements in animals' hepatopancreas and soil; we performed also immunohistochemistry for detection of metallothioneins 1 (MT1) and heat shock protein 70 (HSP70s). The results suggest a great capacity of the species in trace elements bioaccumulation and the high degree of pollution of the area near Agioi Theodoroi where the metal pollution index (MPI) is threefolds higher than the control site, due to the presence of significantly higher concentration of arsenic, cadmium, cobalt, chromium, manganese, molybdenum, and lead found in hepatopancreas and soil. The environmental stress is confirmed by the expression of the relative biomarkers of exposure MT1 and HSP70. Our study, with a multimarker approach, consents getting a complete analysis of environmental quality and the effect of pollution on organisms and shows for the first time the heavy metals contamination status in certain Greece area. Furthermore, P. pruinosus proved to be an appropriate indicator of pollution in terrestrial ecosystems.
Angeliki Droutsa; Nikolaos Emmanouil; Konstantinos Raptopoulos; Antonio Salvaggio; Veronica Mazzei; Roberta Pecoraro; Giuseppina Messina; Chiara Copat; Margherita Ferrante; Maria Violetta Brundo; Bianca Maria Lombardo. Trace elements bioaccumulation in Porcellionides pruinosus (Brandt, 1833) and related biomarkers of exposure. Microscopy Research and Technique 2019, 82, 651 -657.
AMA StyleAngeliki Droutsa, Nikolaos Emmanouil, Konstantinos Raptopoulos, Antonio Salvaggio, Veronica Mazzei, Roberta Pecoraro, Giuseppina Messina, Chiara Copat, Margherita Ferrante, Maria Violetta Brundo, Bianca Maria Lombardo. Trace elements bioaccumulation in Porcellionides pruinosus (Brandt, 1833) and related biomarkers of exposure. Microscopy Research and Technique. 2019; 82 (6):651-657.
Chicago/Turabian StyleAngeliki Droutsa; Nikolaos Emmanouil; Konstantinos Raptopoulos; Antonio Salvaggio; Veronica Mazzei; Roberta Pecoraro; Giuseppina Messina; Chiara Copat; Margherita Ferrante; Maria Violetta Brundo; Bianca Maria Lombardo. 2019. "Trace elements bioaccumulation in Porcellionides pruinosus (Brandt, 1833) and related biomarkers of exposure." Microscopy Research and Technique 82, no. 6: 651-657.
Thiacloprid is an insecticide belonging to the family of neonicotinoids, substances initially underestimated for their potential adverse effects, that they may manifest in the long term leading to an extensive use. The objective of this study was to evaluate the effect at increasing concentrations of thiacloprid on chick embryos development. The research was carried out on 75 fertile eggs of Gallus gallus domesticus. The eggs were opened after 10, 15 and 20 days of incubation and in treated embryos were observed developmental alterations, growth retardation, limbs defects and ectopia viscerale. The histological analysis showed hepatic steatosis and haemorrhages both in the liver and in the lungs. Moreover, the immunohistochemical analysis performed on the liver sections showed a strong positivity only for the erythrocytes to the anti-CYP1A antibody. Thiacloprid exposure increases the risks of teratogenic effects especially at the higher doses tested, therefore its use should be more controlled and limited. Since the literature on the topic is lacking, then the human health impacts resulting from neonicotinoids exposure is not yet fully understood, and, our data will be helpful to allow the assessment of an oral reference dose and health risk characterization.
Antonio Salvaggio; Francesco Antoci; Antonino Messina; Margherita Ferrante; Chiara Copat; Claudia Ruberto; Elena Maria Scalisi; Roberta Pecoraro; Maria Violetta Brundo. Teratogenic effects of the neonicotinoid thiacloprid on chick embryos (Gallus gallus domesticus). Food and Chemical Toxicology 2018, 118, 812 -820.
AMA StyleAntonio Salvaggio, Francesco Antoci, Antonino Messina, Margherita Ferrante, Chiara Copat, Claudia Ruberto, Elena Maria Scalisi, Roberta Pecoraro, Maria Violetta Brundo. Teratogenic effects of the neonicotinoid thiacloprid on chick embryos (Gallus gallus domesticus). Food and Chemical Toxicology. 2018; 118 ():812-820.
Chicago/Turabian StyleAntonio Salvaggio; Francesco Antoci; Antonino Messina; Margherita Ferrante; Chiara Copat; Claudia Ruberto; Elena Maria Scalisi; Roberta Pecoraro; Maria Violetta Brundo. 2018. "Teratogenic effects of the neonicotinoid thiacloprid on chick embryos (Gallus gallus domesticus)." Food and Chemical Toxicology 118, no. : 812-820.
The study aimed to test the cadmium (Cd), copper (Cu) and lead (Pb) bioaccumulation capacity of the marine sponge Chondrilla nucula and to measure the expression of metallothioneins (MTs) by a western blotting assay to explore metal tolerance and its potential use as a bioremediator in contaminated coastal areas. C. nucula was sampled in a protected marine area in order to perform experiments on organisms living in a healthy environment. Under laboratory conditions, the sponges were exposed to increasing concentrations of Cd, Cu and Pb in tanks filled with artificial seawater set up for the experiments. For each metal, three waterborne concentrations (mgL) of Cd (0.02-0.04-0.08), Cu (0.1-0.2-0.4) and Pb (0.2-0.4-0.8) were considered separately. SPSS 20.0 was used for statistical analysis. Significant differences in bioaccumulation across levels of each metal were highlighted. Moreover, the amount accumulated in sponges increased proportionately with increasing levels of exposure for each metal as well as MT expression after Cd and Pb exposure. The results demonstrate the efficiency of C. nucula in accumulating high levels of Cu, but a low tolerance to progressively higher concentrations for Cd and Pb. The study suggests the suitability of the use of this species in bioremediation plans for moderately contaminated environments. However, the biological response in the field should be verified by the use of transplanted organisms from a control site to a polluted site.
Margherita Ferrante; Marilisa Vassallo; Antonio Mazzola; Maria Violetta Brundo; Roberta Pecoraro; Alfina Grasso; Chiara Copat. In vivo exposure of the marine sponge Chondrilla nucula Schmidt, 1862 to cadmium (Cd), copper (Cu) and lead (Pb) and its potential use for bioremediation purposes. Chemosphere 2018, 193, 1049 -1057.
AMA StyleMargherita Ferrante, Marilisa Vassallo, Antonio Mazzola, Maria Violetta Brundo, Roberta Pecoraro, Alfina Grasso, Chiara Copat. In vivo exposure of the marine sponge Chondrilla nucula Schmidt, 1862 to cadmium (Cd), copper (Cu) and lead (Pb) and its potential use for bioremediation purposes. Chemosphere. 2018; 193 ():1049-1057.
Chicago/Turabian StyleMargherita Ferrante; Marilisa Vassallo; Antonio Mazzola; Maria Violetta Brundo; Roberta Pecoraro; Alfina Grasso; Chiara Copat. 2018. "In vivo exposure of the marine sponge Chondrilla nucula Schmidt, 1862 to cadmium (Cd), copper (Cu) and lead (Pb) and its potential use for bioremediation purposes." Chemosphere 193, no. : 1049-1057.
The use of nanomaterials in several application fields has received in the last decades a great attention due to their peculiar properties, but also raised many doubts about possible toxicity when these materials are used for some specific applications, such as water purification. Indeed a careful investigation is needed in order to exclude possible harmful side effects related to the use of nanotechnology. Nanoparticles effects on the marine organisms may depend on their chemical composition, size, surface structure, solubility, shape and how the individual nanoparticles aggregate together. In order to make the most of their potential, without polluting the environment, many researchers are trying to trap them into some kind of matrix that keeps them active but avoids their dispersion in the environment. In this study we have tested nanocomposite membranes prepared using Nafion polymer combined with various fillers, such as anatase-type TiO2 nanoparticles and graphene oxide. The non-toxicity of these nanocomposites, already shown to be effective for water purification applications in our previous studies, was recognized by testing the effect of the different materials on zebrafish embryos. Zebrafish was considered an excellent model for ecotoxicological studies and for this motivation zebrafish embryos were exposed to different concentrations of free nanoparticles and to the nanocomposite membranes. As biomarkers of exposure, we evaluated the expression of heme-oxygenase 1 and inducible Nitric Oxide Synthases by immunohistochemistry and gene expression. Embryo toxicity test showed that nor sublethal effects neither mortality were caused by the different nanoparticles and nano-systems tested. Only zebrafish larvae exposed to free nanoparticles have shown a different response to antibodies anti-heme-oxygenase 1 and anti- inducible Nitric Oxide Synthases. The immunolocalization analysis in fact has highlighted an increase in the synthesis of these biomarkers.
Roberta Pecoraro; Daniele D'Angelo; Simona Filice; Silvia Scalese; Fabiano Capparucci; Fabio Marino; Carmelo Iaria; Giulia Guerriero; Daniele Tibullo; Elena Maria Scalisi; Antonio Salvaggio; Isabella Nicotera; Maria V. Brundo. Toxicity Evaluation of Graphene Oxide and Titania Loaded Nafion Membranes in Zebrafish. Frontiers in Physiology 2018, 8, 1039 .
AMA StyleRoberta Pecoraro, Daniele D'Angelo, Simona Filice, Silvia Scalese, Fabiano Capparucci, Fabio Marino, Carmelo Iaria, Giulia Guerriero, Daniele Tibullo, Elena Maria Scalisi, Antonio Salvaggio, Isabella Nicotera, Maria V. Brundo. Toxicity Evaluation of Graphene Oxide and Titania Loaded Nafion Membranes in Zebrafish. Frontiers in Physiology. 2018; 8 ():1039.
Chicago/Turabian StyleRoberta Pecoraro; Daniele D'Angelo; Simona Filice; Silvia Scalese; Fabiano Capparucci; Fabio Marino; Carmelo Iaria; Giulia Guerriero; Daniele Tibullo; Elena Maria Scalisi; Antonio Salvaggio; Isabella Nicotera; Maria V. Brundo. 2018. "Toxicity Evaluation of Graphene Oxide and Titania Loaded Nafion Membranes in Zebrafish." Frontiers in Physiology 8, no. : 1039.
Nanotechnology is rapidly growing with nanoparticles produced and utilized in a wide range of commercial products worldwide. Among the different types of nanomaterials produced, silver nanoparticles (AgNPs) occupy a predominant position and they are used in electronics, clothing, food industry, cosmetics and medical devices. Nanosilver has also showed excellent performance in antibacterial application. Nowadays, the increasing use of AgNPs has put the evidence on their possible toxicity to the human health and the impact on the environment. This paper focus on adverse effects of AgNPs in adult of Danio rerio. Fishes exposed to increasing concentrations (8, 45, and 70 μg/l) silver nanoparticles (AgNPs, 25 nm in average diameter) and after treatment for 30 days, was quickly euthanized in MS-222. We have evaluated bioaccumulation of AgNPs using ICP-MS and analyzed histological changes, biomarkers of oxidative damage and gene expression in the gut, liver and gills tissues of AgNPs-treated zebrafish. The histological analysis showed lesions of secondary lamellae of the gills with different degrees of toxicity such as hyperplasia, lamellar fusion, subepithelial edema, and even in some cases telangiectasia. Huge necrosis of the intestinal villi was found in the gut. No lesion was detected in the liver. The analysis revealed a high expression of metallothioneins 1 (MTs 1) in animals exposed to AgNPs compared to the control group. The ICP-MS analysis shows that the amount of particles absorbed in all treated samples is almost the same. We can affirm that AgNPs toxicity linked more to their size and state of aggregation than to their concentrations. Silver nanoparticles can damage gills and gut because they are able to pass through the mucosal barrier thanks to their small size. The damage is still reversible because it is not documented injury to the basal membrane.
Roberta Pecoraro; Fabio Marino; Antonio Salvaggio; Fabiano Capparucci; Gianfranco Di Caro; Carmelo Iaria; Andrea Salvo; Archimede Rotondo; Daniele Tibullo; Giulia Guerriero; Elena M. Scalisi; Massimo Zimbone; Giuliana Impellizzeri; Maria V. Brundo. Evaluation of Chronic Nanosilver Toxicity to Adult Zebrafish. Frontiers in Physiology 2017, 8, 1011 .
AMA StyleRoberta Pecoraro, Fabio Marino, Antonio Salvaggio, Fabiano Capparucci, Gianfranco Di Caro, Carmelo Iaria, Andrea Salvo, Archimede Rotondo, Daniele Tibullo, Giulia Guerriero, Elena M. Scalisi, Massimo Zimbone, Giuliana Impellizzeri, Maria V. Brundo. Evaluation of Chronic Nanosilver Toxicity to Adult Zebrafish. Frontiers in Physiology. 2017; 8 ():1011.
Chicago/Turabian StyleRoberta Pecoraro; Fabio Marino; Antonio Salvaggio; Fabiano Capparucci; Gianfranco Di Caro; Carmelo Iaria; Andrea Salvo; Archimede Rotondo; Daniele Tibullo; Giulia Guerriero; Elena M. Scalisi; Massimo Zimbone; Giuliana Impellizzeri; Maria V. Brundo. 2017. "Evaluation of Chronic Nanosilver Toxicity to Adult Zebrafish." Frontiers in Physiology 8, no. : 1011.
Nanomaterials (NM) have different shapes and can be composed of different materials such as carbon, silicon, and some metals like gold, silver, and titanium. They are used as fillers, catalysts, semiconductors, cosmetics, drug carriers in medicine, energy storage systems, and antifriction coatings. NM are the parent compounds of nanoparticles (NPs), which may be divided into two groups: fullerenes and engineered nanoparticles (ENPs). After crossing the cell membrane, NPs may be stored in vesicles, mitochondria, and additional organelles within epithelial cells. They may generate reactive oxygen species, oxidative stress, and cytotoxicity, as well as denaturation of proteins, apoptosis, and necrosis. Nowadays, new toxicological data are required to assess the potential exposure of the environment and human beings to pollutants. The aim of the present investigation is to evaluate the toxicity of the metallic nano-composite by the zebrafish embryo toxicity test (ZFET). The methods described here can be easily adapted to other nanocomposites or nanomaterials with some variations. © 2017 by John Wiley & Sons, Inc.
Roberta Pecoraro; Antonio Salvaggio; Fabio Marino; Gianfranco Di Caro; Fabiano Capparucci; Bianca Maria Lombardo; Giuseppina Messina; Elena Maria Scalisi; Maurizio Tummino; Francesco Loreto; Giusi D'Amante; Roberto Avola; Daniele Tibullo; Maria Violetta Brundo. Metallic Nano‐Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers. Current Protocols in Toxicology 2017, 74, 1.14.1 -1.14.13.
AMA StyleRoberta Pecoraro, Antonio Salvaggio, Fabio Marino, Gianfranco Di Caro, Fabiano Capparucci, Bianca Maria Lombardo, Giuseppina Messina, Elena Maria Scalisi, Maurizio Tummino, Francesco Loreto, Giusi D'Amante, Roberto Avola, Daniele Tibullo, Maria Violetta Brundo. Metallic Nano‐Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers. Current Protocols in Toxicology. 2017; 74 (1):1.14.1-1.14.13.
Chicago/Turabian StyleRoberta Pecoraro; Antonio Salvaggio; Fabio Marino; Gianfranco Di Caro; Fabiano Capparucci; Bianca Maria Lombardo; Giuseppina Messina; Elena Maria Scalisi; Maurizio Tummino; Francesco Loreto; Giusi D'Amante; Roberto Avola; Daniele Tibullo; Maria Violetta Brundo. 2017. "Metallic Nano‐Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers." Current Protocols in Toxicology 74, no. 1: 1.14.1-1.14.13.
Honey is a valuable food produced by bees from sugary substances that they gather in nature. The transformation the nectar into honey, by bees, is long and complex. Except for honey, where heavy metals are absent or are found only in traces, the bees and their products have always been considered excellent biomarkers of such contaminants. We have assumed that the absence of heavy metals in honey is due to the presence of a detoxification system in the digestive system of bees, which involves metallothioneins, proteins that have a role in the homeostatic control of essential and non-essential metals. We have placed the beehives in three different zones: industrial, urban and rural. Investigations were carried out with ICP-MS method for the detection of heavy metals in the guts of honey bees and honey. The metallothioneins have been identified by Immunohistochemical and Western-blotting analisys. The investigations have shown the presence of heavy metals only in bees guts but not in honey, while the presence of metallothionein has been highlighted only in epithelium of the honey sac, demonstrating the existence of an efficient system of detoxification of heavy metals.
Antonio Salvaggio; Roberta Pecoraro; Elena Maria Scalisi; Daniele Tibullo; Bianca Maria Lombardo; Giuseppina Messina; Francesco Loreto; Chiara Copat; Margherita Ferrante; Roberto Avola; Giusi D'Amante; Claudia Genovese; Salvatore Antonino Raccuia; Maria Violetta Brundo. Morphostructural and immunohistochemical study on the role of metallothionein in the detoxification of heavy metals inApis melliferaL., 1758. Microscopy Research and Technique 2017, 80, 1215 -1220.
AMA StyleAntonio Salvaggio, Roberta Pecoraro, Elena Maria Scalisi, Daniele Tibullo, Bianca Maria Lombardo, Giuseppina Messina, Francesco Loreto, Chiara Copat, Margherita Ferrante, Roberto Avola, Giusi D'Amante, Claudia Genovese, Salvatore Antonino Raccuia, Maria Violetta Brundo. Morphostructural and immunohistochemical study on the role of metallothionein in the detoxification of heavy metals inApis melliferaL., 1758. Microscopy Research and Technique. 2017; 80 (11):1215-1220.
Chicago/Turabian StyleAntonio Salvaggio; Roberta Pecoraro; Elena Maria Scalisi; Daniele Tibullo; Bianca Maria Lombardo; Giuseppina Messina; Francesco Loreto; Chiara Copat; Margherita Ferrante; Roberto Avola; Giusi D'Amante; Claudia Genovese; Salvatore Antonino Raccuia; Maria Violetta Brundo. 2017. "Morphostructural and immunohistochemical study on the role of metallothionein in the detoxification of heavy metals inApis melliferaL., 1758." Microscopy Research and Technique 80, no. 11: 1215-1220.
The antibacterial activity and possible toxicity of graphene oxide and laser-irradiated graphene oxide (iGO) were investigated. Antibacterial activity was tested on Escherichia coli and shown to be higher for GO irradiated for at least three hours, which seems to be correlated to the resulting morphology of laser-treated GO and independent of the kind and amount of oxygen functionalities. X-ray photoelectron spectroscopy, Raman spectroscopy, dynamic light scattering and scanning electron microscopy (SEM) show a reduction of the GO flakes size after visible laser irradiation, preserving considerable oxygen content and degree of hydrophilicity. SEM images of the bacteria after the exposure to the iGO flakes confirm membrane damage after interaction with the laser-modified morphology of GO. In addition, a fish embryo toxicity test on zebrafish displayed that neither mortality nor sublethal effects were caused by the different iGO solutions, even when the concentration was increased up to four times higher than the one effective in reducing the bacteria survival. The antibacterial properties and the absence of toxicity make the visible laser irradiation of GO a promising option for water purification applications.
Maria Antonietta Buccheri; Daniele D’Angelo; Silvia Scalese; Simon F Spanò; Simona Filice; Enza Fazio; Giuseppe Compagnini; Massimo Zimbone; Maria Violetta Brundo; Roberta Pecoraro; Anna Alba; Fulvia Sinatra; Giancarlo Rappazzo; Vittorio Privitera. Modification of graphene oxide by laser irradiation: a new route to enhance antibacterial activity. Nanotechnology 2016, 27, 245704 -245704.
AMA StyleMaria Antonietta Buccheri, Daniele D’Angelo, Silvia Scalese, Simon F Spanò, Simona Filice, Enza Fazio, Giuseppe Compagnini, Massimo Zimbone, Maria Violetta Brundo, Roberta Pecoraro, Anna Alba, Fulvia Sinatra, Giancarlo Rappazzo, Vittorio Privitera. Modification of graphene oxide by laser irradiation: a new route to enhance antibacterial activity. Nanotechnology. 2016; 27 (24):245704-245704.
Chicago/Turabian StyleMaria Antonietta Buccheri; Daniele D’Angelo; Silvia Scalese; Simon F Spanò; Simona Filice; Enza Fazio; Giuseppe Compagnini; Massimo Zimbone; Maria Violetta Brundo; Roberta Pecoraro; Anna Alba; Fulvia Sinatra; Giancarlo Rappazzo; Vittorio Privitera. 2016. "Modification of graphene oxide by laser irradiation: a new route to enhance antibacterial activity." Nanotechnology 27, no. 24: 245704-245704.
The increase of heavy metals in the environment involves a high exposure of aquatic organisms to these pollutants. The present study is planned to investigate the effects of zinc chloride (ZnCl2) on the bone embryonic development of Danio rerio and confirm the use of zebrafish as a model organism to study the teratogenic potential of this pollutant. zebrafish embryos were exposed to different ZnCl2 concentrations and analyzed by ICP-MS. The skeletal anomalies were evaluated to confocal microscope after staining with calcein solution and RhodZinTM-3,AM. The data show a delay in hatching compared with the controls, malformations in the process of calcification and significant defects in growth. In conclusion, the current work demonstrates for the first time the Zn toxic effects on calcification process and confirm zebrafish (Danio rerio) as suitable alternative vertebrate model to study the causes and the mechanisms of the skeletal malformations.
Antonio Salvaggio; Fabio Marino; Marco Albano; Roberta Pecoraro; Giuseppina Camiolo; Daniele Tibullo; Vincenzo Bramanti; Bianca M. Lombardo; Salvatore Saccone; Veronica Mazzei; Maria V. Brundo. Toxic Effects of Zinc Chloride on the Bone Development in Danio rerio (Hamilton, 1822). Frontiers in Physiology 2016, 7, 1 .
AMA StyleAntonio Salvaggio, Fabio Marino, Marco Albano, Roberta Pecoraro, Giuseppina Camiolo, Daniele Tibullo, Vincenzo Bramanti, Bianca M. Lombardo, Salvatore Saccone, Veronica Mazzei, Maria V. Brundo. Toxic Effects of Zinc Chloride on the Bone Development in Danio rerio (Hamilton, 1822). Frontiers in Physiology. 2016; 7 ():1.
Chicago/Turabian StyleAntonio Salvaggio; Fabio Marino; Marco Albano; Roberta Pecoraro; Giuseppina Camiolo; Daniele Tibullo; Vincenzo Bramanti; Bianca M. Lombardo; Salvatore Saccone; Veronica Mazzei; Maria V. Brundo. 2016. "Toxic Effects of Zinc Chloride on the Bone Development in Danio rerio (Hamilton, 1822)." Frontiers in Physiology 7, no. : 1.
The effect of the nanoparticles on the marine organisms, depends on their size, chemical composition, surface structure, solubility and shape. In order to take advantage from their activity, preserving the surrounding environment from a possible pollution, we are trying to trap the nanoparticles into new nanomaterials. The nanomaterials tested were synthesized proposing a ground-breaking approach by an upside-down vision of the Au/TiO2nano-system to avoid the release of nanoparticles. The system was synthesized by wrapping Au nanoparticles with a thin layer of TiO2. The non-toxicity of the nano-system was established by testing the effect of the material on zebrafish larvae. Danio rerio o zebrafish was considered a excellent model for the environmental biomonitoring of aquatic environments and the Zebrafish Embryo Toxicity Test is considered an alternative method of animal test. For this reason zebrafish larvae were exposed to different concentrations of nanoparticles of TiO2 and Au and new nanomaterials. As biomarkers of exposure, we evaluated the expression of metallothioneins by immunohistochemistry analysis and western blotting analysis also. The results obtained by toxicity test showed that neither mortality as well as sublethal effects were induced by the different nanomaterials and nanoparticles tested. Only zebrafish larvae exposed to free Au nanoparticles showed a different response to anti-MT antibody. In fact, the immunolocalization analysis highlighted an increase of the metallothioneins synthesis.
Maria Violetta Brundo; Roberta Pecoraro; Fabio Marino; Antonio Salvaggio; Daniele Tibullo; Salvatore Saccone; Vincenzo Bramanti; Maria Antonietta Buccheri; Giuliana Impellizzeri; Viviana Escuderi; Massimo Ezimbone; Vittorio Eprivitera. Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish. Frontiers in Physiology 2016, 7, 130 .
AMA StyleMaria Violetta Brundo, Roberta Pecoraro, Fabio Marino, Antonio Salvaggio, Daniele Tibullo, Salvatore Saccone, Vincenzo Bramanti, Maria Antonietta Buccheri, Giuliana Impellizzeri, Viviana Escuderi, Massimo Ezimbone, Vittorio Eprivitera. Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish. Frontiers in Physiology. 2016; 7 ():130.
Chicago/Turabian StyleMaria Violetta Brundo; Roberta Pecoraro; Fabio Marino; Antonio Salvaggio; Daniele Tibullo; Salvatore Saccone; Vincenzo Bramanti; Maria Antonietta Buccheri; Giuliana Impellizzeri; Viviana Escuderi; Massimo Ezimbone; Vittorio Eprivitera. 2016. "Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish." Frontiers in Physiology 7, no. : 130.
The purpose of this study was to evaluate the extent of the Hg geochemical anomaly arising in the Amiata and Tolfa complex to the coastal area of northern Latium and to examine the possible influence on this area by the Mignone River, and by the small coastal basins, which are characterized by both previous mining activities and decades of past industrial impact. The results confirm the extension of the anomaly of concentrations of Hg in the coastal area of northern Latium, with the northern sector influenced by the contributions of the Fiora and Mignone Rivers and the southern sector influenced by the contributions of minor basins. The results show high values of the Adverse Effect Index throughout the considered area and highlight the need for further investigation in order to assess the impact of human activities on the present and past values of Hg in marine sediments.
Sergio Scanu; Daniele Piazzolla; Francesco Manfredi Frattarelli; Emanuele Mancini; Francesco Tiralongo; Maria Violetta Brundo; Daniele Tibullo; Roberta Pecoraro; Chiara Copat; Margherita Ferrante; Marco Marcelli. Mercury Enrichment in Sediments of the Coastal Area of Northern Latium, Italy. Bulletin of Environmental Contamination and Toxicology 2016, 96, 630 -637.
AMA StyleSergio Scanu, Daniele Piazzolla, Francesco Manfredi Frattarelli, Emanuele Mancini, Francesco Tiralongo, Maria Violetta Brundo, Daniele Tibullo, Roberta Pecoraro, Chiara Copat, Margherita Ferrante, Marco Marcelli. Mercury Enrichment in Sediments of the Coastal Area of Northern Latium, Italy. Bulletin of Environmental Contamination and Toxicology. 2016; 96 (5):630-637.
Chicago/Turabian StyleSergio Scanu; Daniele Piazzolla; Francesco Manfredi Frattarelli; Emanuele Mancini; Francesco Tiralongo; Maria Violetta Brundo; Daniele Tibullo; Roberta Pecoraro; Chiara Copat; Margherita Ferrante; Marco Marcelli. 2016. "Mercury Enrichment in Sediments of the Coastal Area of Northern Latium, Italy." Bulletin of Environmental Contamination and Toxicology 96, no. 5: 630-637.