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Nearly 40–50% of infertility problems are estimated to be of female origin. Previous studies dedicated to the analysis of metabolites in follicular fluid (FF) produced contrasting results, although some valuable indexes capable to discriminate control groups (CTRL) from infertile females (IF) and correlate with outcome measures of assisted reproduction techniques were in some instances found. In this study, we analyzed in blind FF of 35 control subjects (CTRL = patients in which inability to obtain pregnancy was exclusively due to a male factor) and 145 IF (affected by: endometriosis, n = 19; polycystic ovary syndrome, n = 14; age-related reduced ovarian reserve, n = 58; reduced ovarian reserve, n = 29; unexplained infertility, n = 14; genetic infertility, n = 11) to determine concentrations of 55 water- and fat-soluble low molecular weight compounds (antioxidants, oxidative/nitrosative stress-related compounds, purines, pyrimidines, energy-related metabolites, and amino acids). Results evidenced that 27/55 of them had significantly different values in IF with respect to those measured in CTRL. The metabolic pattern of these potential biomarkers of infertility was cumulated (in both CTRL and IF) into a Biomarker Score index (incorporating the metabolic anomalies of FF), that fully discriminated CTRL (mean Biomarker Score value = 4.00 ± 2.30) from IF (mean Biomarker Score value = 14.88 ± 3.09, p< 0.001). The Biomarker Score values were significantly higher than those of CTRL in each of the six subgroups of IF. Posterior probability curves and ROC curve indicated that values of the Biomarker Score clustered CTRL and IF into two distinct groups, based on the individual FF metabolic profile. Furthermore, Biomarker Score values correlated with outcome measures of ovarian stimulation, in vitro fertilization, number and quality of blastocysts, clinical pregnancy, and healthy offspring. These results strongly suggest that the biochemical quality of FF deeply influences not only the effectiveness of IVF procedures but also the following embryonic development up to healthy newborns. The targeted metabolomic analysis of FF (using empowered Redox Energy Test) and the subsequent calculation of the Biomarker Score evidenced a set of 27 low molecular weight infertility biomarkers potentially useful in the laboratory managing of female infertility and to predict the success of assisted reproduction techniques.
Giacomo Lazzarino; Romina Pallisco; Gabriele Bilotta; Ilaria Listorti; Renata Mangione; Miriam Wissam Saab; Giuseppe Caruso; Angela Maria Amorini; Maria Violetta Brundo; Giuseppe Lazzarino; Barbara Tavazzi; Pasquale Bilotta. Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization. International Journal of Molecular Sciences 2021, 22, 8735 .
AMA StyleGiacomo Lazzarino, Romina Pallisco, Gabriele Bilotta, Ilaria Listorti, Renata Mangione, Miriam Wissam Saab, Giuseppe Caruso, Angela Maria Amorini, Maria Violetta Brundo, Giuseppe Lazzarino, Barbara Tavazzi, Pasquale Bilotta. Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization. International Journal of Molecular Sciences. 2021; 22 (16):8735.
Chicago/Turabian StyleGiacomo Lazzarino; Romina Pallisco; Gabriele Bilotta; Ilaria Listorti; Renata Mangione; Miriam Wissam Saab; Giuseppe Caruso; Angela Maria Amorini; Maria Violetta Brundo; Giuseppe Lazzarino; Barbara Tavazzi; Pasquale Bilotta. 2021. "Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization." International Journal of Molecular Sciences 22, no. 16: 8735.
The focus of this work was to investigate the toxicity of different metal nanoparticles (gold nanoparticles [AuNPs], silver nanoparticles [AgNPs], titanium dioxide nanoparticles [TiO2NPs]) on brine shrimp Artemia salina. We investigated if nanoparticles could have an influence on hatching of cysts and on mortality of larvae. Larvae (also called nauplii) and cysts were exposed to NPs for 24 hr in artificial seawater on microplates. At the end of the test, we assessed the endpoint (immobility/death) for the larvae by a stereomicroscope. Nauplii that appeared completely motionless, were counted as dead, and the percentages of mortality were calculated for each treatment. Instead for the cysts, the percentages of not‐hatched nauplii for each concentration considered were calculated by counting the number of whole cysts. Currently, nanoparticles toxicity has been investigated in several research; in our study we highlighted the nontoxicity of TiO2NPs on A. salina nauplii as shown by low percentages of immobilization and on cysts because TiO2NPs do not affect their hatching. Despite AuNPs exerted toxic effects on hatching, they did not affect larvae development as well as TiO2NPs. Otherwise, AgNPs induced mortality of the larvae and inhibited cysts hatching.
Roberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Giuseppe Fragalà; Sara Ignoto; Antonio Salvaggio; Massimo Zimbone; Giuliana Impellizzeri; Maria Violetta Brundo. Artemia salina : A microcrustacean to assess engineered nanoparticles toxicity. Microscopy Research and Technique 2020, 84, 531 -536.
AMA StyleRoberta Pecoraro, Elena Maria Scalisi, Giuseppina Messina, Giuseppe Fragalà, Sara Ignoto, Antonio Salvaggio, Massimo Zimbone, Giuliana Impellizzeri, Maria Violetta Brundo. Artemia salina : A microcrustacean to assess engineered nanoparticles toxicity. Microscopy Research and Technique. 2020; 84 (3):531-536.
Chicago/Turabian StyleRoberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Giuseppe Fragalà; Sara Ignoto; Antonio Salvaggio; Massimo Zimbone; Giuliana Impellizzeri; Maria Violetta Brundo. 2020. "Artemia salina : A microcrustacean to assess engineered nanoparticles toxicity." Microscopy Research and Technique 84, no. 3: 531-536.
Pollution and other types of environmental stress do not spare marine environments, especially those affected by high industrial pressure. Fish, especially coastal species, are used for monitoring the marine environment because they are particularly efficient as bioindicators thanks to their ability to bioaccumulate and biomagnify along the trophic chain. The aim of this research is to evaluate the bioaccumulation and the indirect bioindication ability of the European Hake, Merluccius merluccius (Linnaeus, 1758), one of the most important commercial fish species of the Mediterranean Sea. Morphological and histological alterations of the main target organs, such as liver and gills, have been investigated and the results showed a steatosis in the hepatic tissue. The accumulation of heavy metals has been analyzed by inductively coupled plasma mass spectrometry and for several metals it was showed a different concentration in the two sexes. Moreover, the expression of metallothioneins 1 and Heat Shock Protein 70 has been assessed by immunohistochemistry and did not show high level of expression. We underline the importance of contamination evaluation in commercial fish species and the utilization of the ichthyofauna as bioindicator of environmental quality.
Antonio Salvaggio; Roberta Pecoraro; Chiara Copat; Margherita Ferrante; Alfina Grasso; Elena Maria Scalisi; Sara Ignoto; Vincenza Serena Bonaccorsi; Giuseppina Messina; Bianca Maria Lombardo; Francesco Tiralongo; Maria Violetta Brundo. Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment. Journal of Marine Science and Engineering 2020, 8, 712 .
AMA StyleAntonio Salvaggio, Roberta Pecoraro, Chiara Copat, Margherita Ferrante, Alfina Grasso, Elena Maria Scalisi, Sara Ignoto, Vincenza Serena Bonaccorsi, Giuseppina Messina, Bianca Maria Lombardo, Francesco Tiralongo, Maria Violetta Brundo. Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment. Journal of Marine Science and Engineering. 2020; 8 (9):712.
Chicago/Turabian StyleAntonio Salvaggio; Roberta Pecoraro; Chiara Copat; Margherita Ferrante; Alfina Grasso; Elena Maria Scalisi; Sara Ignoto; Vincenza Serena Bonaccorsi; Giuseppina Messina; Bianca Maria Lombardo; Francesco Tiralongo; Maria Violetta Brundo. 2020. "Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment." Journal of Marine Science and Engineering 8, no. 9: 712.
Organophosphates are a large class of chemicals with anticholinesterase action insecticides. Dimethoate belongs to the class of organophosphates and it is used for agriculture purpose. Its main toxicological role in animals and humans is the inhibition of the activity of acetylcholinesterase. Although it is not considered genotoxic, carcinogenic and teratogen, there is evidence of increased pup mortality in developmental neurotoxicity studies. Since there is scant published literature about developmental toxicity, we investigated the adverse effects of dimethoate on fertilization and embryonic development in sea urchin (Paracentrotus lividus), a model organism widely used to assess the toxicity of contaminants on environmental matrices; so pesticide residues can be released into the environment, and could affect the health of organisms, including humans. Different solution of dimethoate (4 × 10−3, 4 × 10−4, 4 × 10−5, 4 × 10−6 and 4 × 10−7 g/10 ml) have been tested on spermatozoa of P. lividus to evaluate the fertilizing ability of them when we added egg cells untreated. We demonstrated that dimethoate does not interfere with fertilizing ability of spermatozoa but egg cells fertilized by treated spermatozoa showed alterations in the segmentation planes as asymmetric and/or asynchronous cell divisions.
Elena Maria Scalisi; Roberta Pecoraro; Antonio Salvaggio; Aurora Corsaro; Giuseppina Messina; Sara Ignoto; Bianca Maria Lombardo; Maria Violetta Brundo. Evaluation of dimethoate toxicity on fertilization and on embryonic development of Paracentrotus lividus (Lamarck, 1816). Toxicology Research 2020, 9, 1 .
AMA StyleElena Maria Scalisi, Roberta Pecoraro, Antonio Salvaggio, Aurora Corsaro, Giuseppina Messina, Sara Ignoto, Bianca Maria Lombardo, Maria Violetta Brundo. Evaluation of dimethoate toxicity on fertilization and on embryonic development of Paracentrotus lividus (Lamarck, 1816). Toxicology Research. 2020; 9 (4):1.
Chicago/Turabian StyleElena Maria Scalisi; Roberta Pecoraro; Antonio Salvaggio; Aurora Corsaro; Giuseppina Messina; Sara Ignoto; Bianca Maria Lombardo; Maria Violetta Brundo. 2020. "Evaluation of dimethoate toxicity on fertilization and on embryonic development of Paracentrotus lividus (Lamarck, 1816)." Toxicology Research 9, no. 4: 1.
In many Vertebrate and invertebrate, the interaction mechanisms among gametes are based on a high affinity and specificity of recognition and link between specific saccharidic residues and receptors present on the surface of gametes. Literature data also suggest that Insects could use this strategy. In particular, Orthoptera Tettigoniidae spermatodesms and sperms undergo radical changes passing through the male to the female genital tracts that may be interpreted as well as a capacitation process. These changes could also concern the presence and distribution of surface glycoconjugates. Our study aims to highlight the presence and distribution of saccharide residues on the spermatozoa surface in five species of Orthoptera Tettigoniidae using a battery of lectins Fluorescein Isothiocyanate Conjugated and Gold Conjugated. The results of this investigation have shown that on the surface of the male gametes are present saccharide residues whose nature and distribution are species‐specific, during their transfer to the female genital tract, they significantly undergo a change. These results let us hypothesize that also for this group of Insects, the glycoconjugates play a significant role in the process of interaction between gametes.
Roberta Pecoraro; Elena Maria Scalisi; Maria Violetta Brundo. Lectin binding sites on the plasma membranes of Orthoptera Tettigonioidea spermatodesms. Microscopy Research and Technique 2020, 83, 1321 -1329.
AMA StyleRoberta Pecoraro, Elena Maria Scalisi, Maria Violetta Brundo. Lectin binding sites on the plasma membranes of Orthoptera Tettigonioidea spermatodesms. Microscopy Research and Technique. 2020; 83 (11):1321-1329.
Chicago/Turabian StyleRoberta Pecoraro; Elena Maria Scalisi; Maria Violetta Brundo. 2020. "Lectin binding sites on the plasma membranes of Orthoptera Tettigonioidea spermatodesms." Microscopy Research and Technique 83, no. 11: 1321-1329.
Recently two-dimensional nanomaterials, such as graphene and molybdenum disulfide (MoS2), have received much attention as adsorbent materials for the effective removal of organic contaminants. MoS2 is attracting attention, not only for its chemical-physical properties, but also for its wide availability in nature as a constituent of molybdenite. The aim of this investigation was to assess the effects of different MoS2 concentrations (5 × 10−1, 5 × 10−2 and 5 × 10−3 mg/ml) on the embryonated eggs of Gallus gallus domesticus, according to Beck method. We evaluated the toxic effect of the MoS2 powder purchased at Sigma-Aldrich indicated as “received” and MoS2 powder treated via mechanical milling indicated as “ball mille”. Subsequently, the embryos were sacrificed at different times of embryonic development (11th, 15th and 19th day after incubation) in order to evaluate their embryotoxic and teratogenic effects. The alterations of the embryonic development were studied by morphological and immunohistochemical analysis of the tissues. The results obtained have shown the toxicity of both powders of MoS2 with a high percentage of deaths and growth delays. Moreover, the immunohistochemical analysis performed on several tissue sections showed a strong positivity to the anti-metallothionein1 antibody only for the erythrocytes.
Elena Maria Scalisi; Antonio Salvaggio; Francesco Antoci; Antonio Messina; Roberta Pecoraro; Maria Cantarella; Giuliana Gorrasi; Giuliana Impellizzeri; Maria Violetta Brundo. Toxicity assessment of two-dimensional nanomaterials molybdenum disulfide in Gallus gallus domesticus. Ecotoxicology and Environmental Safety 2020, 200, 110772 .
AMA StyleElena Maria Scalisi, Antonio Salvaggio, Francesco Antoci, Antonio Messina, Roberta Pecoraro, Maria Cantarella, Giuliana Gorrasi, Giuliana Impellizzeri, Maria Violetta Brundo. Toxicity assessment of two-dimensional nanomaterials molybdenum disulfide in Gallus gallus domesticus. Ecotoxicology and Environmental Safety. 2020; 200 ():110772.
Chicago/Turabian StyleElena Maria Scalisi; Antonio Salvaggio; Francesco Antoci; Antonio Messina; Roberta Pecoraro; Maria Cantarella; Giuliana Gorrasi; Giuliana Impellizzeri; Maria Violetta Brundo. 2020. "Toxicity assessment of two-dimensional nanomaterials molybdenum disulfide in Gallus gallus domesticus." Ecotoxicology and Environmental Safety 200, no. : 110772.
The proteasome inhibitor bortezomib (BTZ) has emerged as an effective drug for the treatment of multiple myeloma even though many patients relapse from BTZ therapy. The present study investigated the metabolic pathways underlying the acquisition of bortezomib resistance in multiple myeloma. We used two different clones of multiple myeloma cell lines exhibiting different sensitivities to BTZ (U266 and U266-R) and compared them in terms of metabolic profile, mitochondrial fitness and redox balance homeostasis capacity. Our results showed that the BTZ-resistant clone (U266-R) presented increased glycosylated UDP-derivatives when compared to BTZ-sensitive cells (U266), thus also suggesting higher activities of the hexosamine biosynthetic pathway (HBP), regulating not only protein O- and N-glycosylation but also mitochondrial functions. Notably, U266-R displayed increased mitochondrial biogenesis and mitochondrial dynamics associated with stronger antioxidant defenses. Furthermore, U266-R maintained a significantly higher concentration of substrates for protein glycosylation when compared to U266, particularly for UDP-GlcNac, thus further suggesting the importance of glycosylation in the BTZ pharmacological response. Moreover, BTZ-treated U266-R showed significantly higher ATP/ADP ratios and levels of ECP and also exhibited increased mitochondrial fitness and antioxidant response. In conclusions, our findings suggest that the HBP may play a major role in mitochondrial fitness, driving BTZ resistance in multiple myeloma and thus representing a possible target for new drug development for BTZ-resistant patients.
Daniele Tibullo; Cesarina Giallongo; Alessandra Romano; Nunzio Vicario; Alessandro Barbato; Fabrizio Puglisi; Rosalba Parenti; Angela Maria Amorini; Miriam Wissam Saab; Barbara Tavazzi; Renata Mangione; Maria Violetta Brundo; Giacomo Lazzarino; Giuseppe Alberto Palumbo; Giovanni Li Volti; Francesco Di Raimondo; Giuseppe Lazzarino. Mitochondrial Functions, Energy Metabolism and Protein Glycosylation are Interconnected Processes Mediating Resistance to Bortezomib in Multiple Myeloma Cells. Biomolecules 2020, 10, 696 .
AMA StyleDaniele Tibullo, Cesarina Giallongo, Alessandra Romano, Nunzio Vicario, Alessandro Barbato, Fabrizio Puglisi, Rosalba Parenti, Angela Maria Amorini, Miriam Wissam Saab, Barbara Tavazzi, Renata Mangione, Maria Violetta Brundo, Giacomo Lazzarino, Giuseppe Alberto Palumbo, Giovanni Li Volti, Francesco Di Raimondo, Giuseppe Lazzarino. Mitochondrial Functions, Energy Metabolism and Protein Glycosylation are Interconnected Processes Mediating Resistance to Bortezomib in Multiple Myeloma Cells. Biomolecules. 2020; 10 (5):696.
Chicago/Turabian StyleDaniele Tibullo; Cesarina Giallongo; Alessandra Romano; Nunzio Vicario; Alessandro Barbato; Fabrizio Puglisi; Rosalba Parenti; Angela Maria Amorini; Miriam Wissam Saab; Barbara Tavazzi; Renata Mangione; Maria Violetta Brundo; Giacomo Lazzarino; Giuseppe Alberto Palumbo; Giovanni Li Volti; Francesco Di Raimondo; Giuseppe Lazzarino. 2020. "Mitochondrial Functions, Energy Metabolism and Protein Glycosylation are Interconnected Processes Mediating Resistance to Bortezomib in Multiple Myeloma Cells." Biomolecules 10, no. 5: 696.
Three different Advanced Oxidation Processes (AOPs) have been investigated for the degradation of the imidacloprid pesticide in water: photocatalysis, Fenton and photo-Fenton reactions. For these tests, we have compared the performance of two types of CeO2, employed as a non-conventional photocatalyst/Fenton-like material. The first one has been prepared by chemical precipitation with KOH, while the second one has been obtained by exposing the as-synthetized CeO2 to solar irradiation in H2 stream. This latter treatment led to obtain a more defective CeO2 (coded as “grey CeO2”) with the formation of Ce3+ sites on the surface of CeO2, as determined by Raman and X-ray Photoelectron Spectroscopy (XPS) characterizations. This peculiar feature has been demonstrated as beneficial for the solar photo–Fenton reaction, with the best performance exhibited by the grey CeO2. On the contrary, the bare CeO2 showed a photocatalytic activity higher with respect to the grey CeO2, due to the higher exposed surface area and the lower band-gap. The easy synthetic procedures of CeO2 reported here, allows to tune and modify the physico-chemical properties of CeO2, allowing a choice of different CeO2 samples on the basis of the specific AOPs for water remediation. Furthermore, neither of the samples have shown any critical toxicity.
Roberto Fiorenza; Stefano Andrea Balsamo; Luisa D’Urso; Salvatore Sciré; Maria Violetta Brundo; Roberta Pecoraro; Elena Maria Scalisi; Vittorio Privitera; Giuliana Impellizzeri. CeO2 for Water Remediation: Comparison of Various Advanced Oxidation Processes. Catalysts 2020, 10, 446 .
AMA StyleRoberto Fiorenza, Stefano Andrea Balsamo, Luisa D’Urso, Salvatore Sciré, Maria Violetta Brundo, Roberta Pecoraro, Elena Maria Scalisi, Vittorio Privitera, Giuliana Impellizzeri. CeO2 for Water Remediation: Comparison of Various Advanced Oxidation Processes. Catalysts. 2020; 10 (4):446.
Chicago/Turabian StyleRoberto Fiorenza; Stefano Andrea Balsamo; Luisa D’Urso; Salvatore Sciré; Maria Violetta Brundo; Roberta Pecoraro; Elena Maria Scalisi; Vittorio Privitera; Giuliana Impellizzeri. 2020. "CeO2 for Water Remediation: Comparison of Various Advanced Oxidation Processes." Catalysts 10, no. 4: 446.
The aim of this study was to evaluate the toxicity of titanium dioxide nanoparticles (TiO2NPs) by short‐term toxicity tests in Apis mellifera, considered an excellent bioindicator organism mainly due to its sensitivity. Bees have been exposed to several concentrations of TiO2NPs (1 × 10−3, 1 × 10−4, 1 × 10−5, 1 × 10−6 mg/10 ml) for 10 days. Morphostructural and histological assays were done on gut and honey sac. The research of exposure biomarkers like metallothioneins 1 (MT1) and Heat Shock Protein 70 (HSP70) was performed to verify if a detoxification mechanism has been activated in the exposed animals. No histological alteration on the epithelium of the gut and honey sac were observed in exposed samples. A significant positivity for anti‐MT1 antibody was observed only in the honey sac cells. A weak positivity for HSP70 was observed in both structures analyzed. In several studies have shown the non‐toxicity of TiO2NPs on other model organisms, in our study, titanium dioxide nanoparticles was proven to be highly toxic at the highest concentration tested (100% of lethality to 1 × 10−3 mg/10 ml) and moderately toxic at lower concentrations. Honey bees proved to be excellent models for study of NPs toxicity and for monitoring environment.
Giulia Ferrara; Antonio Salvaggio; Roberta Pecoraro; Elena Maria Scalisi; Andrea Maria Presti; Giuliana Impellizzeri; Maria Violetta Brundo. Toxicity assessment of nano‐TiO 2 in Apis mellifera L., 1758: histological and immunohistochemical assays. Microscopy Research and Technique 2019, 83, 332 -337.
AMA StyleGiulia Ferrara, Antonio Salvaggio, Roberta Pecoraro, Elena Maria Scalisi, Andrea Maria Presti, Giuliana Impellizzeri, Maria Violetta Brundo. Toxicity assessment of nano‐TiO 2 in Apis mellifera L., 1758: histological and immunohistochemical assays. Microscopy Research and Technique. 2019; 83 (4):332-337.
Chicago/Turabian StyleGiulia Ferrara; Antonio Salvaggio; Roberta Pecoraro; Elena Maria Scalisi; Andrea Maria Presti; Giuliana Impellizzeri; Maria Violetta Brundo. 2019. "Toxicity assessment of nano‐TiO 2 in Apis mellifera L., 1758: histological and immunohistochemical assays." Microscopy Research and Technique 83, no. 4: 332-337.
Invertebrates are precious organisms in order to study environmental pollution. In particular, they appear to be suitable as a bioindicator species for pioneer ecotoxicity studies on new xenobiotics such as nanoparticles. In fact, they are able to absorb nanomaterials scattered in the environment in different ways and it's known the compartmentalization of nano‐sized contaminants in selected tissues and intracellular organelles. Titanium dioxide represents the most used nanoparticulate, destined to become probably ubiquitous in the environment. Recently, some research has been published on the toxic potential of nano‐TiO2 in several animal species. Among all invertebrates, Oniscidean Isopods are the only taxon of Crustaceans that has become completely terrestrial, known as excellent bioindicators and bioaccumulators. They have a digestive gland, the hepatopancreas, which is the location of election for the accumulation of pollutants. For this reason, they are considered efficient animal models to ecological studies. For this study, we collected Armadillo officinalis from Natural Oriented Reserve of “Vendicari” (Sicily, Italy), to evaluate the toxicity of titanium dioxide (TiO2) on their hepatopancreas, after a short period of exposure. We conducted morphostructural and immunohistochemistry assays. The results suggested a great capacity of the species of bioaccumulation of nanoparticles in the hepatopancreas, where a strong positivity to the metallothioneins was highlighted. Our study confirms that Oniscidean Isopods, in particular Armadillo officinalis, proved to be an appropriate indicator of pollution in terrestrial ecosystems from nanoparticles.
Alessia Flaccavento; Roberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Antonio Salvaggio; Giuliana Impellizzeri; Bianca Maria Lombardo; Maria Violetta Brundo. Morphostructural and immunohistochemical study for evaluation of nano‐TiO 2 toxicity in Armadillo officinalis Duméril, 1816 (Crustacea, Isopoda, Oniscidea). Microscopy Research and Technique 2019, 83, 297 -303.
AMA StyleAlessia Flaccavento, Roberta Pecoraro, Elena Maria Scalisi, Giuseppina Messina, Antonio Salvaggio, Giuliana Impellizzeri, Bianca Maria Lombardo, Maria Violetta Brundo. Morphostructural and immunohistochemical study for evaluation of nano‐TiO 2 toxicity in Armadillo officinalis Duméril, 1816 (Crustacea, Isopoda, Oniscidea). Microscopy Research and Technique. 2019; 83 (3):297-303.
Chicago/Turabian StyleAlessia Flaccavento; Roberta Pecoraro; Elena Maria Scalisi; Giuseppina Messina; Antonio Salvaggio; Giuliana Impellizzeri; Bianca Maria Lombardo; Maria Violetta Brundo. 2019. "Morphostructural and immunohistochemical study for evaluation of nano‐TiO 2 toxicity in Armadillo officinalis Duméril, 1816 (Crustacea, Isopoda, Oniscidea)." Microscopy Research and Technique 83, no. 3: 297-303.
In order to achieve a selective removal of specific pesticides from water, we synthesized, through the sol-gel technique, molecularly imprinted TiO2 photocatalysts with the only use of the standard reactants of the TiO2 sol-gel synthesis together with the pesticide molecules, without any addition of further reactants supports or matrices. It is a new, easy, smart and scalable method that avoid the multistep and solvent-consuming procedures, typical of the molecular imprinting. Two widely-used pesticides, i.e. the herbicide 2,4D, and the insecticide imidacloprid, were chosen as template for the molecular imprinting and as contaminants target for the photocatalytic tests. A remarkable enhancement of the photocatalytic activity was verified with the TiO2 imprinted with the corresponding pesticide-target. The selectivity of the photodegradation process was verified thanks to the comparison with the degradation of pesticides not-used as template. Furthermore, the eventual toxic effects of the molecularly imprinted materials were evaluated by biological tests. The combination of molecular imprinting with photocatalysis, here investigated for the first time with pesticides, it is a promising strategy to selectively catch (through the molecular imprinting process) and degrade (through the photocatalysis) specific organic contaminants from water.
Roberto Fiorenza; Alessandro Di Mauro; Maria Cantarella; Carmelo Iaria; Elena Maria Scalisi; Maria Violetta Brundo; Antonino Gulino; Luca Spitaleri; Giuseppe Nicotra; Sandro Dattilo; Sabrina Carola Carroccio; Vittorio Privitera; Giuliana Impellizzeri. Preferential removal of pesticides from water by molecular imprinting on TiO2 photocatalysts. Chemical Engineering Journal 2019, 379, 122309 .
AMA StyleRoberto Fiorenza, Alessandro Di Mauro, Maria Cantarella, Carmelo Iaria, Elena Maria Scalisi, Maria Violetta Brundo, Antonino Gulino, Luca Spitaleri, Giuseppe Nicotra, Sandro Dattilo, Sabrina Carola Carroccio, Vittorio Privitera, Giuliana Impellizzeri. Preferential removal of pesticides from water by molecular imprinting on TiO2 photocatalysts. Chemical Engineering Journal. 2019; 379 ():122309.
Chicago/Turabian StyleRoberto Fiorenza; Alessandro Di Mauro; Maria Cantarella; Carmelo Iaria; Elena Maria Scalisi; Maria Violetta Brundo; Antonino Gulino; Luca Spitaleri; Giuseppe Nicotra; Sandro Dattilo; Sabrina Carola Carroccio; Vittorio Privitera; Giuliana Impellizzeri. 2019. "Preferential removal of pesticides from water by molecular imprinting on TiO2 photocatalysts." Chemical Engineering Journal 379, no. : 122309.
In recent years, the Mediterranean Sea has become an accumulation zone for waste generated by the 22 countries bordering its shores. Although the effects of plastic litter on the marine environment and on organisms have recently been studied in other areas, further information is needed for the Mediterranean Sea and, in particular, about plastics additives inputs and interactions with the biota and the trophic network, such as phthalates and bisphenol A. Plastic material production, use and disposal contribute also to the release of heavy metals into the environment, such as mercury (Hg), often used during the production of chlorine, the primary ingredient in PVC, lead (Pb) and cadmium (Cd), which are used as stabilizers in PVC and leach out of products during use and disposal. Our research aims to evaluate phthalates, bisphenol A and heavy metals contamination in Lepidopus caudatus (Pisces, Trichiuridae), which could be considered as a potential sentinel species. For the evaluation of toxicological effects, we evaluated the expression of vitellogenin and metallothioneins 1. In all samples analyzed, we have not found microplastics in the gastrointestinal tract but chemical analysis revealed the presence of high content of phthalates, and in particular high quantities of DIDP, DEHP, bis-benzylester phthalate, bis-butyl ester phthalate and mono-N-butyl ester phthalate in different organs. Instead, trace elements detected in tissue revealed a trend of concentrations generally higher in liver and intestine than gill and muscle tissues. Immunohistochemical analysis for anti-metallothionein 1 antibody showed a strong positivity of liver cells, both in females and males. Analysis for the anti-vitellogenin antibody showed in females a strong positivity both in the liver cells and in the gonads, in male specimens was found to be always negative except for a specimen, in which it was highlighted a positivity in some areas of the liver and of the gonad.
Antonio Salvaggio; Francesco Tiralongo; Evangelia Krasakopoulou; Dimitra Marmara; Ioannis Giovos; Rosalia Crupi; Giuseppina Messina; Bianca Maria Lombardo; Alessandra Marzullo; Roberta Pecoraro; Elena Maria Scalisi; Chiara Copat; Pietro Zuccarello; Margherita Ferrante; Maria Violetta Brundo. Biomarkers of Exposure to Chemical Contamination in the Commercial Fish Species Lepidopus caudatus (Euphrasen, 1788): A Particular Focus on Plastic Additives. Frontiers in Physiology 2019, 10, 905 .
AMA StyleAntonio Salvaggio, Francesco Tiralongo, Evangelia Krasakopoulou, Dimitra Marmara, Ioannis Giovos, Rosalia Crupi, Giuseppina Messina, Bianca Maria Lombardo, Alessandra Marzullo, Roberta Pecoraro, Elena Maria Scalisi, Chiara Copat, Pietro Zuccarello, Margherita Ferrante, Maria Violetta Brundo. Biomarkers of Exposure to Chemical Contamination in the Commercial Fish Species Lepidopus caudatus (Euphrasen, 1788): A Particular Focus on Plastic Additives. Frontiers in Physiology. 2019; 10 ():905.
Chicago/Turabian StyleAntonio Salvaggio; Francesco Tiralongo; Evangelia Krasakopoulou; Dimitra Marmara; Ioannis Giovos; Rosalia Crupi; Giuseppina Messina; Bianca Maria Lombardo; Alessandra Marzullo; Roberta Pecoraro; Elena Maria Scalisi; Chiara Copat; Pietro Zuccarello; Margherita Ferrante; Maria Violetta Brundo. 2019. "Biomarkers of Exposure to Chemical Contamination in the Commercial Fish Species Lepidopus caudatus (Euphrasen, 1788): A Particular Focus on Plastic Additives." Frontiers in Physiology 10, no. : 905.
Although in recent years there has been intensification in the use of nanoparticles (NPs) for the production of many commercial products, few studies have been carried out to assess the risks associated with its use. Among the most used NPs, silver nanoparticles (AgNPs) have a preponderant position as they have various applications. In this study, 40 adult zebrafish were exposed to increasing concentrations of AgNPs (8, 45, and 70 μg/L) for 30 days to evaluate the effects on eyes after chronic exposure to AgNPs with an average diameter of 50 nm. From the morphological and ultrastructural analysis performed, no alteration or lesions of the corneal epithelium were detected.
Roberta Pecoraro; Antonio Salvaggio; Elena Maria Scalisi; Carmelo Iaria; Giovanni Lanteri; Chiara Copat; Margherita Ferrante; Giuseppe Fragalà; Massimo Zimbone; Giuliana Impellizzeri; Maria V. Brundo. Evaluation of the effects of silver nanoparticles on Danio rerio cornea: Morphological and ultrastructural analysis. Microscopy Research and Technique 2019, 82, 1297 -1301.
AMA StyleRoberta Pecoraro, Antonio Salvaggio, Elena Maria Scalisi, Carmelo Iaria, Giovanni Lanteri, Chiara Copat, Margherita Ferrante, Giuseppe Fragalà, Massimo Zimbone, Giuliana Impellizzeri, Maria V. Brundo. Evaluation of the effects of silver nanoparticles on Danio rerio cornea: Morphological and ultrastructural analysis. Microscopy Research and Technique. 2019; 82 (8):1297-1301.
Chicago/Turabian StyleRoberta Pecoraro; Antonio Salvaggio; Elena Maria Scalisi; Carmelo Iaria; Giovanni Lanteri; Chiara Copat; Margherita Ferrante; Giuseppe Fragalà; Massimo Zimbone; Giuliana Impellizzeri; Maria V. Brundo. 2019. "Evaluation of the effects of silver nanoparticles on Danio rerio cornea: Morphological and ultrastructural analysis." Microscopy Research and Technique 82, no. 8: 1297-1301.
Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the present study was to assess the iron chelating properties of α-lipoic acid (ALA) and how such an effect impacts on iron overload mediated toxicity. Human mesenchymal stem cells (HS-5) and animals (zebrafish, n = 10 for each group) were treated for 24 h with ferric ammonium citrate (FAC, 120 µg/mL) in the presence or absence of ALA (20 µg/mL). Oxidative stress was evaluated by reduced glutathione content, reactive oxygen species formation, mitochondrial dysfunction, and gene expression of heme oxygenase-1b and mitochondrial superoxide dismutase; organ injury, iron accumulation, and autophagy were measured by microscopical, cytofluorimetric analyses, and inductively coupled plasma‒optical mission Spectrometer (ICP-OES). Our results showed that FAC results in a significant increase of tissue iron accumulation, oxidative stress, and autophagy and such detrimental effects were reversed by ALA treatment. In conclusion, ALA possesses excellent iron chelating properties that may be exploited in a clinical setting for organ preservation, as well as exhibiting a good safety profile and low cost for the national health system.
Giuseppina Camiolo; Daniele Tibullo; Cesarina Giallongo; Alessandra Romano; Nunziatina L. Parrinello; Giuseppe Musumeci; Michelino Di Rosa; Nunzio Vicario; Maria V. Brundo; Francesco Amenta; Margherita Ferrante; Chiara Copat; Roberto Avola; Giovanni Li Volti; Antonio Salvaggio; Francesco Di Raimondo; Giuseppe A. Palumbo. α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload. International Journal of Molecular Sciences 2019, 20, 609 .
AMA StyleGiuseppina Camiolo, Daniele Tibullo, Cesarina Giallongo, Alessandra Romano, Nunziatina L. Parrinello, Giuseppe Musumeci, Michelino Di Rosa, Nunzio Vicario, Maria V. Brundo, Francesco Amenta, Margherita Ferrante, Chiara Copat, Roberto Avola, Giovanni Li Volti, Antonio Salvaggio, Francesco Di Raimondo, Giuseppe A. Palumbo. α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload. International Journal of Molecular Sciences. 2019; 20 (3):609.
Chicago/Turabian StyleGiuseppina Camiolo; Daniele Tibullo; Cesarina Giallongo; Alessandra Romano; Nunziatina L. Parrinello; Giuseppe Musumeci; Michelino Di Rosa; Nunzio Vicario; Maria V. Brundo; Francesco Amenta; Margherita Ferrante; Chiara Copat; Roberto Avola; Giovanni Li Volti; Antonio Salvaggio; Francesco Di Raimondo; Giuseppe A. Palumbo. 2019. "α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload." International Journal of Molecular Sciences 20, no. 3: 609.
Two-dimensional (2D) nanomaterials have received much attention in recent years, because of their unusual properties associated with their ultra-thin thickness and 2D morphology. Besides graphene, a new 2D material, molybdenum disulfide (MoS2), has attracted immense interest in various applications. On the other hand, ball-milling process provides an original strategy to modify materials at the nanometer scale. This methodology represents a smart solution for the fabrication of MoS2 nanopowders extremely-efficient in adsorbing water contaminants in aqueous solution. This work reports a comprehensive morphological, structural, and physicochemical investigation of MoS2 nanopowders treated with dry ball-milling. The adsorption performances of the produced nanopowders were tested using methylene blue (MB) dye and phenol in aqueous solution. The adsorption capacity as a function of ball-milling time was deeply studied and explained. Importantly, the ball-milled MoS2 nanopowders can be easily and efficiently regenerated without compromising their adsorption capacity, so to be reusable for dye adsorption. The eventual toxic effects of the prepared materials on microcrustacean Artemia salina were also studied. The present results demonstrate that ball-milling of MoS2 offers a valid method for large-scale production of extremely efficient adsorbent for the decontamination of wastewaters from several pollutants.
Maria Cantarella; Giuliana Gorrasi; Alessandro Di Mauro; Mario Scuderi; Giuseppe Nicotra; Roberto Fiorenza; Salvatore Scirè; Elena Maria Scalisi; Maria Violetta Brundo; Vittorio Privitera; Giuliana Impellizzeri. Mechanical milling: a sustainable route to induce structural transformations in MoS2 for applications in the treatment of contaminated water. Scientific Reports 2019, 9, 1 -14.
AMA StyleMaria Cantarella, Giuliana Gorrasi, Alessandro Di Mauro, Mario Scuderi, Giuseppe Nicotra, Roberto Fiorenza, Salvatore Scirè, Elena Maria Scalisi, Maria Violetta Brundo, Vittorio Privitera, Giuliana Impellizzeri. Mechanical milling: a sustainable route to induce structural transformations in MoS2 for applications in the treatment of contaminated water. Scientific Reports. 2019; 9 (1):1-14.
Chicago/Turabian StyleMaria Cantarella; Giuliana Gorrasi; Alessandro Di Mauro; Mario Scuderi; Giuseppe Nicotra; Roberto Fiorenza; Salvatore Scirè; Elena Maria Scalisi; Maria Violetta Brundo; Vittorio Privitera; Giuliana Impellizzeri. 2019. "Mechanical milling: a sustainable route to induce structural transformations in MoS2 for applications in the treatment of contaminated water." Scientific Reports 9, no. 1: 1-14.
In the last years, the increase of anthropogenic activities has led to a progressive pollution of ecosystems. Oniscidean isopods are a group of terrestrial animals known as excellent bio‐indicators and bio‐accumulators, because of their capacity to accumulate contaminants and to implement appropriate strategies of detoxification. For this study, we collected Porcellionides pruinosus from two different sites in the Corinth region (Greece), a polluted site and a control site, to assess the heavy metal pollution status. We conducted an inductively coupled plasma mass spectrometry (ICP‐MS) analysis for the detection of trace elements in animals' hepatopancreas and soil; we performed also immunohistochemistry for detection of metallothioneins 1 (MT1) and heat shock protein 70 (HSP70s). The results suggest a great capacity of the species in trace elements bioaccumulation and the high degree of pollution of the area near Agioi Theodoroi where the metal pollution index (MPI) is threefolds higher than the control site, due to the presence of significantly higher concentration of arsenic, cadmium, cobalt, chromium, manganese, molybdenum, and lead found in hepatopancreas and soil. The environmental stress is confirmed by the expression of the relative biomarkers of exposure MT1 and HSP70. Our study, with a multimarker approach, consents getting a complete analysis of environmental quality and the effect of pollution on organisms and shows for the first time the heavy metals contamination status in certain Greece area. Furthermore, P. pruinosus proved to be an appropriate indicator of pollution in terrestrial ecosystems.
Angeliki Droutsa; Nikolaos Emmanouil; Konstantinos Raptopoulos; Antonio Salvaggio; Veronica Mazzei; Roberta Pecoraro; Giuseppina Messina; Chiara Copat; Margherita Ferrante; Maria Violetta Brundo; Bianca Maria Lombardo. Trace elements bioaccumulation in Porcellionides pruinosus (Brandt, 1833) and related biomarkers of exposure. Microscopy Research and Technique 2019, 82, 651 -657.
AMA StyleAngeliki Droutsa, Nikolaos Emmanouil, Konstantinos Raptopoulos, Antonio Salvaggio, Veronica Mazzei, Roberta Pecoraro, Giuseppina Messina, Chiara Copat, Margherita Ferrante, Maria Violetta Brundo, Bianca Maria Lombardo. Trace elements bioaccumulation in Porcellionides pruinosus (Brandt, 1833) and related biomarkers of exposure. Microscopy Research and Technique. 2019; 82 (6):651-657.
Chicago/Turabian StyleAngeliki Droutsa; Nikolaos Emmanouil; Konstantinos Raptopoulos; Antonio Salvaggio; Veronica Mazzei; Roberta Pecoraro; Giuseppina Messina; Chiara Copat; Margherita Ferrante; Maria Violetta Brundo; Bianca Maria Lombardo. 2019. "Trace elements bioaccumulation in Porcellionides pruinosus (Brandt, 1833) and related biomarkers of exposure." Microscopy Research and Technique 82, no. 6: 651-657.
Environmental pollution is a topic of great interest because it directly affects the quality of ecosystems and of all living organisms at different trophic and systematic levels. Together with the global climate change, the long-term surviving of many species of plants and animals is threaten, distributional patterns at global and regional levels are altered and it results in local assemblages of species that are quite different from those that currently constitute coevolved communities. .For this study, the species Myotis myotis was used as bioindicator and it was sampled from two caves in the south-east of Sicily, Pipistrelli chosen as control area and Palombara chosen as polluted area, to measure the concentrations of trace elements in fur and liver tissues. Results showed higher content of essential elements in fur in bats sampled from Pipistrelli. Conversely, higher concentrations of toxic metals in liver such as As, Cd, Pb and Hg were measured in bat samples in Palombara cave, where specimens have a hunting area extended within the boundaries of the petrochemical plant. Nevertheless, we cannot consider Palombara population as polluted by metal contamination since their tissue concentrations are overall lower than toxic thresholds values suggested for small mammals. Likewise, we cannot exclude other kind of pollutants as potential stressors of the examined population, contributing with the decreasing of bat colonies in Sicily.
Margherita Ferrante; Maria Teresa Spena; Béatrice Veronique Hernout; Alfina Grasso; Andrea Messina; Rosario Grasso; Paolo Agnelli; Maria Violetta Brundo; Chiara Copat. Trace elements bioaccumulation in liver and fur of Myotis myotis from two caves of the eastern side of Sicily (Italy): A comparison between a control and a polluted area. Environmental Pollution 2018, 240, 273 -285.
AMA StyleMargherita Ferrante, Maria Teresa Spena, Béatrice Veronique Hernout, Alfina Grasso, Andrea Messina, Rosario Grasso, Paolo Agnelli, Maria Violetta Brundo, Chiara Copat. Trace elements bioaccumulation in liver and fur of Myotis myotis from two caves of the eastern side of Sicily (Italy): A comparison between a control and a polluted area. Environmental Pollution. 2018; 240 ():273-285.
Chicago/Turabian StyleMargherita Ferrante; Maria Teresa Spena; Béatrice Veronique Hernout; Alfina Grasso; Andrea Messina; Rosario Grasso; Paolo Agnelli; Maria Violetta Brundo; Chiara Copat. 2018. "Trace elements bioaccumulation in liver and fur of Myotis myotis from two caves of the eastern side of Sicily (Italy): A comparison between a control and a polluted area." Environmental Pollution 240, no. : 273-285.
Thiacloprid is an insecticide belonging to the family of neonicotinoids, substances initially underestimated for their potential adverse effects, that they may manifest in the long term leading to an extensive use. The objective of this study was to evaluate the effect at increasing concentrations of thiacloprid on chick embryos development. The research was carried out on 75 fertile eggs of Gallus gallus domesticus. The eggs were opened after 10, 15 and 20 days of incubation and in treated embryos were observed developmental alterations, growth retardation, limbs defects and ectopia viscerale. The histological analysis showed hepatic steatosis and haemorrhages both in the liver and in the lungs. Moreover, the immunohistochemical analysis performed on the liver sections showed a strong positivity only for the erythrocytes to the anti-CYP1A antibody. Thiacloprid exposure increases the risks of teratogenic effects especially at the higher doses tested, therefore its use should be more controlled and limited. Since the literature on the topic is lacking, then the human health impacts resulting from neonicotinoids exposure is not yet fully understood, and, our data will be helpful to allow the assessment of an oral reference dose and health risk characterization.
Antonio Salvaggio; Francesco Antoci; Antonino Messina; Margherita Ferrante; Chiara Copat; Claudia Ruberto; Elena Maria Scalisi; Roberta Pecoraro; Maria Violetta Brundo. Teratogenic effects of the neonicotinoid thiacloprid on chick embryos (Gallus gallus domesticus). Food and Chemical Toxicology 2018, 118, 812 -820.
AMA StyleAntonio Salvaggio, Francesco Antoci, Antonino Messina, Margherita Ferrante, Chiara Copat, Claudia Ruberto, Elena Maria Scalisi, Roberta Pecoraro, Maria Violetta Brundo. Teratogenic effects of the neonicotinoid thiacloprid on chick embryos (Gallus gallus domesticus). Food and Chemical Toxicology. 2018; 118 ():812-820.
Chicago/Turabian StyleAntonio Salvaggio; Francesco Antoci; Antonino Messina; Margherita Ferrante; Chiara Copat; Claudia Ruberto; Elena Maria Scalisi; Roberta Pecoraro; Maria Violetta Brundo. 2018. "Teratogenic effects of the neonicotinoid thiacloprid on chick embryos (Gallus gallus domesticus)." Food and Chemical Toxicology 118, no. : 812-820.
ROS concentration, measured by ESR on isolated frog skin, through the presented non-lethal method, is a reliable biomarker for toxicity screening and represents a useful basic datum for future modelling studies on environmental monitoring and biodiversity loss prevention.
Gerardino D’Errico; Giuseppe Vitiello; Gaetano De Tommaso; Fagr Kh. Abdel-Gawad; Maria Violetta Brundo; Margherita Ferrante; Anna De Maio; Samantha Trocchia; Anna Rita Bianchi; Gaetano Ciarcia; Giulia Guerriero. Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring. Environmental Research 2018, 165, 11 -18.
AMA StyleGerardino D’Errico, Giuseppe Vitiello, Gaetano De Tommaso, Fagr Kh. Abdel-Gawad, Maria Violetta Brundo, Margherita Ferrante, Anna De Maio, Samantha Trocchia, Anna Rita Bianchi, Gaetano Ciarcia, Giulia Guerriero. Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring. Environmental Research. 2018; 165 ():11-18.
Chicago/Turabian StyleGerardino D’Errico; Giuseppe Vitiello; Gaetano De Tommaso; Fagr Kh. Abdel-Gawad; Maria Violetta Brundo; Margherita Ferrante; Anna De Maio; Samantha Trocchia; Anna Rita Bianchi; Gaetano Ciarcia; Giulia Guerriero. 2018. "Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring." Environmental Research 165, no. : 11-18.
Here, we report the industrial pollution effects studies as it pertains to cadmium on the reproductive health of Mytilus galloprovincialis. Mussels were removed from the biofouling of Conatex pannel after one year of exposition in a polluted site close to a disposal metallurgical factory. In testis of mussels housed in the polluted site, was observed, respect to a reference control site, a high cadmium bioaccumulation by inductively coupled plasma-mass spectrometry, and a 10 fold increase metallothionein 20 kDa gene (mt20) expression levels, by qPCR. Furthermore, mussels transferred in laboratory tanks from the reference site and exposed to 1.5, 5 and 10 µM CdCl2 revealed 1.7; 3.2 and 4.5 fold testis mt20 expression increase respectively and a positive correlation with cadmium bioaccumulation. To evaluate the potential detrimental risk of such alterations on spermatozoa we carried out electrophoretic analyses on their protamine-like proteins. Protamine-like proteins from the polluted biofouling resulted all in aggregated form and showed by EMSA, the same decreased DNA binding ability of protamine-like proteins obtained from mussels exposed in laboratory tank to CdCl2. Our results contribute to the studies concerning cadmium induced testis alterations and highlight protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on Mytilus galloprovincialis for the sensibility of changes in vivo and in vitro of protamine-like proteins state and their DNA binding affinity.
Marina Piscopo; Marco Trifuoggi; Rosaria Notariale; Sofiane Labar; Jacopo Troisi; Antonella Giarra; Dea Rabbito; Raffaela Puoti; Maria Violetta Brundo; Adriana Basile; Juan Ausió; Giulia Guerriero. Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819). Acta Biochimica Polonica 2018, 65, 259 -267.
AMA StyleMarina Piscopo, Marco Trifuoggi, Rosaria Notariale, Sofiane Labar, Jacopo Troisi, Antonella Giarra, Dea Rabbito, Raffaela Puoti, Maria Violetta Brundo, Adriana Basile, Juan Ausió, Giulia Guerriero. Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819). Acta Biochimica Polonica. 2018; 65 (2):259-267.
Chicago/Turabian StyleMarina Piscopo; Marco Trifuoggi; Rosaria Notariale; Sofiane Labar; Jacopo Troisi; Antonella Giarra; Dea Rabbito; Raffaela Puoti; Maria Violetta Brundo; Adriana Basile; Juan Ausió; Giulia Guerriero. 2018. "Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)." Acta Biochimica Polonica 65, no. 2: 259-267.