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Natural dyes have been used from ancient times for multiple purposes, most importantly in the field of textile dying. The increasing demand and excessive costs of natural dye extraction engendered the discovery of synthetic dyes from petrochemical compounds. Nowadays, they are dominating the textile market, with nearly 8 × 105 tons produced per year due to their wide range of color pigments and consistent coloration. Textile industries consume huge amounts of water in the dyeing processes, making it hard to treat the enormous quantities of this hazardous wastewater. Thus, they have harmful impacts when discharged in non-treated or partially treated forms in the environment (air, soil, plants and water), causing several human diseases. In the present work we focused on synthetic dyes. We started by studying their classification which depended on the nature of the manufactured fiber (cellulose, protein and synthetic fiber dyes). Then, we mentioned the characteristics of synthetic dyes, however, we focused more on their negative impacts on the ecosystem (soil, plants, water and air) and on humans. Lastly, we discussed the applied physical, chemical and biological strategies solely or in combination for textile dye wastewater treatments. Additionally, we described the newly established nanotechnology which achieves complete discharge decontamination.
Houda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences 2021, 11, 6255 .
AMA StyleHouda Ben Slama, Ali Chenari Bouket, Zeinab Pourhassan, Faizah N. Alenezi, Allaoua Silini, Hafsa Cherif-Silini, Tomasz Oszako, Lenka Luptakova, Patrycja Golińska, Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences. 2021; 11 (14):6255.
Chicago/Turabian StyleHouda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. 2021. "Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods." Applied Sciences 11, no. 14: 6255.
Diverse vegetable oils are extracted from oleagenic trees and plants all over the world. In particular, olive oil represents a strategic socio-economic branch in the Mediterranean countries. These countries use either two or three-phase olive oil extraction systems. In this work, we focus on the by-products from three-phase olive oil extraction, which are the liquid olive mill wastewater (OMW) and the solid olive mill pomace (OMP) rejected in evaporative ponds. The disposal of this recalcitrant waste poses environmental problems such as the death of different species of insects and animals. In-depth ICP-OES analysis of the heavy metal composition of OMW and OMP revealed the presence of many metals ranging from non-toxic to highly toxic. The LC-HRMS characterization of these by-products indicated the presence of several secondary metabolites harmful to humans or to the environment. Thus, we aimed to identify OMW and OMP indigenous microbiota through metagenomics. The bacterial population was dominated by the Acetobacter (49.7%), Gluconobacter (17.3%), Gortzia (13.7%) and Nardonalla (5.3%) genera. The most abundant fungal genera were Nakazawaea, Saccharomyces, Lachancea and Candida. These microbial genera are responsible for OMW, OMP and soil toxicity alleviation.
Houda Ben Slama; Ali Chenari Bouket; Faizah N. Alenezi; Ameur Khardani; Lenka Luptakova; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation. Applied Sciences 2021, 11, 5131 .
AMA StyleHouda Ben Slama, Ali Chenari Bouket, Faizah N. Alenezi, Ameur Khardani, Lenka Luptakova, Armelle Vallat, Tomasz Oszako, Mostafa E. Rateb, Lassaad Belbahri. Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation. Applied Sciences. 2021; 11 (11):5131.
Chicago/Turabian StyleHouda Ben Slama; Ali Chenari Bouket; Faizah N. Alenezi; Ameur Khardani; Lenka Luptakova; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. 2021. "Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation." Applied Sciences 11, no. 11: 5131.
A modified one-step process was used to prepare tetracalcium phosphate/monetite/calcium sulfate hemihydrate powder cement mixtures (CAS). The procedure allowed the formation of monetite and calcium sulfate hemihydrate (CSH) in the form of nanoparticles. It was hypothesized that the presence of nanoCSH in small amounts enhances the in vitro bioactivity of CAS cement in relation to osteogenic gene markers in mesenchymal stem cells (MSCs). The CAS powder mixtures with 15 and 5 wt.% CSH were prepared by milling powder tetracalcium phosphate in an ethanolic solution of both orthophosphoric and sulfuric acids. The CAS cements had short setting times (around 5 min). The fast setting of the cement samples after the addition of the liquid component (water solution of NaH2PO4) was due to the partial formation of calcium sulfate dihydrate and hydroxyapatite before soaking in SBF with a small change in the original phase composition in cement powder samples after milling. Nanocrystalline hydroxyapatite biocement was produced by soaking of cement samples after setting in simulated body fluid (SBF). The fast release of calcium ions from CAS5 cement, as well as a small rise in the pH of SBF during soaking, were demonstrated. After soaking in SBF for 7 days, the final product of the cement transformation was nanocrystalline hydroxyapatite. The compressive strength of the cement samples (up to 30 MPa) after soaking in simulated body fluid (SBF) was comparable to that of bone. Real time polymerase chain reaction (RT-PCR) analysis revealed statistically significant higher gene expressions of alkaline phosphatase (ALP), osteonectin (ON) and osteopontin (OP) in cells cultured for 14 days in CAS5 extract compared to CSH-free cement. The addition of a small amount of nanoCSH (5 wt.%) to the tetracalcium phosphate (TTCP)/monetite cement mixture significantly promoted the over expression of osteogenic markers in MSCs. The prepared CAS powder mixture with its enhanced bioactivity can be used for bone defect treatment and has good potential for bone healing.
Lubomir Medvecky; Maria Giretova; Radoslava Stulajterova; Lenka Luptakova; Tibor Sopcak. Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation. Materials 2021, 14, 2137 .
AMA StyleLubomir Medvecky, Maria Giretova, Radoslava Stulajterova, Lenka Luptakova, Tibor Sopcak. Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation. Materials. 2021; 14 (9):2137.
Chicago/Turabian StyleLubomir Medvecky; Maria Giretova; Radoslava Stulajterova; Lenka Luptakova; Tibor Sopcak. 2021. "Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation." Materials 14, no. 9: 2137.
Plant growth promoting bacteria (PGPB) have been the target of intensive research studies toward their efficient use in the field as biofertilizers, biocontrol, and bioremediation agents among numerous other applications. Recent trends in the field of PGPB research led to the development of versatile multifaceted PGPB that can be used in different field conditions such as biocontrol of plant pathogens in metal contaminated soils. Unfortunately, all these research efforts lead to the development of PGPB that failed to perform in salty environments. Therefore, it is urgently needed to address this drawback of these PGPB toward their efficient performance in salinity context. In this paper we provide a review of state-of-the-art research in the field of PGPB and propose a road map for the development of next generation versatile and multifaceted PGPB that can perform in salinity. Beyond soil desalinization, our study paves the way towards the development of PGPB able to provide services in diverse salty environments such as heavy metal contaminated, or pathogen threatened. Smart development of salinity adapted next generation biofertilizers will inevitably allow for mitigation and alleviation of biotic and abiotic threats to plant productivity in salty environments.
Hafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability 2021, 13, 4422 .
AMA StyleHafsa Cherif-Silini, Allaoua Silini, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Nawel Bouremani, Justyna Anna Nowakowska, Tomasz Oszako, Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability. 2021; 13 (8):4422.
Chicago/Turabian StyleHafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. 2021. "Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application." Sustainability 13, no. 8: 4422.
Biopolymer composites allow the creation of an optimal environment for the regeneration of chondral and osteochondral defects of articular cartilage, where natural regeneration potential is limited. In this experimental study, we used the sheep animal model for the creation of knee cartilage defects. In the medial part of the trochlea and on the medial condyle of the femur, we created artificial defects (6 × 3 mm2) with microfractures. In four experimental sheep, both defects were subsequently filled with the porous acellular polyhydroxybutyrate/chitosan (PHB/CHIT)-based implant. Two sheep had untreated defects. We evaluated the quality of the newly formed tissue in the femoral trochlea defect site using imaging (X-ray, Computer Tomography (CT), Magnetic Resonance Imaging (MRI)), macroscopic, and histological methods. Macroscopically, the surface of the treated regenerate corresponded to the niveau of the surrounding cartilage. X-ray examination 6 months after the implantation confirmed the restoration of the contour in the subchondral calcified layer and the advanced rate of bone tissue integration. The CT scan revealed a low regenerative potential in the bone zone of the defect compared to the cartilage zone. The percentage change in cartilage density at the defect site was not significantly different to the reference area (0.06–6.4%). MRI examination revealed that the healing osteochondral defect was comparable to the intact cartilage signal on the surface of the defect. Hyaline-like cartilage was observed in most of the treated animals, except for one, where the defect was repaired with fibrocartilage. Thus, the acellular, chitosan-based biomaterial is a promising biopolymer composite for the treatment of chondral and osteochondral defects of traumatic character. It has potential for further clinical testing in the orthopedic field, primarily with the combination of supporting factors.
Eva Petrovova; Marek Tomco; Katarina Holovska; Jan Danko; Lenka Kresakova; Katarina Vdoviakova; Veronika Simaiova; Filip Kolvek; Petra Hornakova; Teodor Toth; Jozef Zivcak; Peter Gal; David Sedmera; Lenka Luptakova; Lubomir Medvecky. PHB/CHIT Scaffold as a Promising Biopolymer in the Treatment of Osteochondral Defects—An Experimental Animal Study. Polymers 2021, 13, 1232 .
AMA StyleEva Petrovova, Marek Tomco, Katarina Holovska, Jan Danko, Lenka Kresakova, Katarina Vdoviakova, Veronika Simaiova, Filip Kolvek, Petra Hornakova, Teodor Toth, Jozef Zivcak, Peter Gal, David Sedmera, Lenka Luptakova, Lubomir Medvecky. PHB/CHIT Scaffold as a Promising Biopolymer in the Treatment of Osteochondral Defects—An Experimental Animal Study. Polymers. 2021; 13 (8):1232.
Chicago/Turabian StyleEva Petrovova; Marek Tomco; Katarina Holovska; Jan Danko; Lenka Kresakova; Katarina Vdoviakova; Veronika Simaiova; Filip Kolvek; Petra Hornakova; Teodor Toth; Jozef Zivcak; Peter Gal; David Sedmera; Lenka Luptakova; Lubomir Medvecky. 2021. "PHB/CHIT Scaffold as a Promising Biopolymer in the Treatment of Osteochondral Defects—An Experimental Animal Study." Polymers 13, no. 8: 1232.
Dimethyl sulfoxide (DMSO) is widely used as a solvent for small hydrophobic drug molecules. However, the safe volume allowing to avoid its embryotoxic effect has been poorly studied. In this study, we documented the effects of dimethyl sulfoxide (DMSO) in the developing chicken embryo at morphological, histological, and molecular levels. We focused on the developing chicken liver as the main organ involved in the process of detoxification. In our study, 100% DMSO was administered subgerminally onto the eggshell membrane (membrana papyracea) at various volumes (5, 10, 15, 20, 25, 30, 35, and 50 µL) on 4th embryonic day (ED). We focused on histopathological alterations of the liver structure, and noticed the overall impact of DMSO on developing chicken embryos (embryotoxicity, malformation). At the molecular level, we studied cytochrome P450 complex (CYP) isoform’s activities in relation to changes of CYP1A5, CYP3A37, and CYP3A80 gene expression. Total embryotoxicity after application of different doses of DMSO on ED 4, and the embryo lethality increased with increasing DMSO amounts. Overall mortality after DMSO administration ranged below 33%. Mortality was increased with higher amounts of DMSO, mainly from 20 µL. The highest mortality was observed for the highest dose of DMSO over 35 µL. The results also showed a decrease in body weight with increased application volumes of DMSO. At the histological level, we observed mainly the presence of lipid droplets and dilated bile canaliculi and sinusoids in samples over the administration of 25 µL of DMSO. While these findings were not statistically significant, DMSO treatment caused a significant different up-regulation of mRNA expression in all studied genes. For CYP1A5, CYP3A37, and CYP3A80 DMSO volumes needed were 15 µL, 10 µL, and 20 µL, respectively. A significant down-regulation of all studied CYP isoform was detected after application of a DMSO dose of 5 µL. Regarding the morphological results, we can assume that the highest safe dose of DMSO without affecting chicken embryo development and its liver is up to 10 µL. This conclusion is corroborated with the presence of number of malformations and body weight reduction, which correlates with histological findings. Moreover, the gene expression results showed that even the lowest administered DMSO volume could affect hepatocytes at the molecular level causing down-regulation of cytochrome P450 complex (CYP1A5, CYP3A37, CYP3A80).
Lenka Luptakova; Simona Dvorcakova; Zuzana Demcisakova; Lassaad Belbahri; Katarina Holovska; Eva Petrovova. Dimethyl Sulfoxide: Morphological, Histological, and Molecular View on Developing Chicken Liver. Toxics 2021, 9, 55 .
AMA StyleLenka Luptakova, Simona Dvorcakova, Zuzana Demcisakova, Lassaad Belbahri, Katarina Holovska, Eva Petrovova. Dimethyl Sulfoxide: Morphological, Histological, and Molecular View on Developing Chicken Liver. Toxics. 2021; 9 (3):55.
Chicago/Turabian StyleLenka Luptakova; Simona Dvorcakova; Zuzana Demcisakova; Lassaad Belbahri; Katarina Holovska; Eva Petrovova. 2021. "Dimethyl Sulfoxide: Morphological, Histological, and Molecular View on Developing Chicken Liver." Toxics 9, no. 3: 55.
Novel calcium phosphate cements containing a mixture of four amino acids, glycine, proline, hydroxyproline and either lysine or arginine (CAL, CAK) were characterized and used for treatment of artificial osteochondral defects in knee. It was hypothesized that an enhanced concentration of extracellular collagen amino acids (in complex mixture), in connection with bone cement in defect sites, would support the healing of osteochondral defects with successful formation of hyaline cartilage and subchondral bone. Calcium phosphate cement mixtures were prepared by in situ reaction in a planetary ball mill at aseptic conditions and characterized. It was verified that about 30–60% of amino acids remained adsorbed on hydroxyapatite particles in cements and the addition of amino acids caused around 60% reduction in compressive strength and refinement of hydroxyapatite particles in their microstructure. The significant over-expression of osteogenic genes after the culture of osteoblasts was demonstrated in the cement extracts containing lysine and compared with other cements. The cement pastes were inserted into artificial osteochondral defects in the medial femoral condyle of pigs and, after 3 months post-surgery, tissues were analyzed macroscopically, histologically, immunohistochemically using MRI and X-ray methods. Analysis clearly showed the excellent healing process of artificial osteochondral defects in pigs after treatment with CAL and CAK cements without any inflammation, as well as formation of subchondral bone and hyaline cartilage morphologically and structurally identical to the original tissues. Good integration of the hyaline neocartilage with the surrounding tissue, as well as perfect interconnection between the neocartilage and new subchondral bone tissue, was demonstrated. Tissues were stable after 12 months’ healing.
Lubomir Medvecky; Maria Giretova; Radoslava Stulajterova; Jan Danko; Katarina Vdoviakova; Lenka Kresakova; Zdenek Zert; Eva Petrovova; Katarina Holovska; Maros Varga; Lenka Luptakova; Tibor Sopcak. Characterization of Properties, In Vitro and In Vivo Evaluation of Calcium Phosphate/Amino Acid Cements for Treatment of Osteochondral Defects. Materials 2021, 14, 436 .
AMA StyleLubomir Medvecky, Maria Giretova, Radoslava Stulajterova, Jan Danko, Katarina Vdoviakova, Lenka Kresakova, Zdenek Zert, Eva Petrovova, Katarina Holovska, Maros Varga, Lenka Luptakova, Tibor Sopcak. Characterization of Properties, In Vitro and In Vivo Evaluation of Calcium Phosphate/Amino Acid Cements for Treatment of Osteochondral Defects. Materials. 2021; 14 (2):436.
Chicago/Turabian StyleLubomir Medvecky; Maria Giretova; Radoslava Stulajterova; Jan Danko; Katarina Vdoviakova; Lenka Kresakova; Zdenek Zert; Eva Petrovova; Katarina Holovska; Maros Varga; Lenka Luptakova; Tibor Sopcak. 2021. "Characterization of Properties, In Vitro and In Vivo Evaluation of Calcium Phosphate/Amino Acid Cements for Treatment of Osteochondral Defects." Materials 14, no. 2: 436.
Marine endophytic fungi from under-explored locations are a promising source for the discovery of new bioactivities. Different endophytic fungi were isolated from plants and marine organisms collected from Wadi El-Natrun saline lakes and the Red Sea near Hurghada, Egypt. The isolated strains were grown on three different media, and their ethyl acetate crude extracts were evaluated for their antimicrobial activity against a panel of pathogenic bacteria and fungi as well as their antioxidant properties. Results showed that most of the 32 fungal isolates initially obtained possessed antimicrobial and antioxidant activities. The most potent antimicrobial extracts were applied to three different cellulose containing fabrics to add new multifunctional properties such as ultraviolet protection and antimicrobial functionality. For textile safety, the toxicity profile of the selected fungal extract was evaluated on human fibroblasts. The 21 strains displaying bioactivity were identified on molecular basis and selected for chemical screening and dereplication, which was carried out by analysis of the MS/MS data using the Global Natural Products Social Molecular Networking (GNPS) platform. The obtained molecular network revealed molecular families of compounds commonly produced by fungal strains, and in combination with manual dereplication, further previously reported metabolites were identified as well as potentially new derivatives.
Ahmed Hamed; Sylvia Soldatou; M. Qader; Subha Arjunan; Kevin Miranda; Federica Casolari; Coralie Pavesi; Oluwatofunmilay Diyaolu; Bathini Thissera; Manal Eshelli; Lassaad Belbahri; Lenka Luptakova; Nabil Ibrahim; Mohamed Abdel-Aziz; Basma Eid; Mosad Ghareeb; Mostafa Rateb; Rainer Ebel. Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles. Microorganisms 2020, 8, 1617 .
AMA StyleAhmed Hamed, Sylvia Soldatou, M. Qader, Subha Arjunan, Kevin Miranda, Federica Casolari, Coralie Pavesi, Oluwatofunmilay Diyaolu, Bathini Thissera, Manal Eshelli, Lassaad Belbahri, Lenka Luptakova, Nabil Ibrahim, Mohamed Abdel-Aziz, Basma Eid, Mosad Ghareeb, Mostafa Rateb, Rainer Ebel. Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles. Microorganisms. 2020; 8 (10):1617.
Chicago/Turabian StyleAhmed Hamed; Sylvia Soldatou; M. Qader; Subha Arjunan; Kevin Miranda; Federica Casolari; Coralie Pavesi; Oluwatofunmilay Diyaolu; Bathini Thissera; Manal Eshelli; Lassaad Belbahri; Lenka Luptakova; Nabil Ibrahim; Mohamed Abdel-Aziz; Basma Eid; Mosad Ghareeb; Mostafa Rateb; Rainer Ebel. 2020. "Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles." Microorganisms 8, no. 10: 1617.
(1) Background: The preparation and characterization of novel fully injectable enzymatically hardened tetracalcium phosphate/monetite cements (CXI cements) using phytic acid/phytase (PHYT/F3P) hardening liquid with a small addition of polyacrylic acid/carboxymethyl cellulose anionic polyelectrolyte (PAA/CMC) and enhanced bioactivity. (2) Methods: Composite cements were prepared by mixing of calcium phosphate powder mixture with hardening liquid containing anionic polyelectrolyte. Phase and microstructural analysis, compressive strength, release of ions and in vitro testing were used for the evaluation of cement properties. (3) Results: The simple possibility to control the setting time of self-setting CXI cements was shown (7–28 min) by the change in P/L ratio or PHYT/F3P reaction time. The wet compressive strength of cements (up to 15 MPa) was close to cancellous bone. The increase in PAA content to 1 wt% caused refinement and change in the morphology of hydroxyapatite particles. Cement pastes had a high resistance to wash-out in a short time after cement mixing. The noncytotoxic character of CX cement extracts was verified. Moreover, PHYT supported the formation of Ca deposits, and the additional synergistic effect of PAA and CMC on enhanced ALP activity was found, along with the strong up-regulation of osteogenic gene expressions for osteopontin, osteocalcin and IGF1 growth factor evaluated by the RT-qPCR analysis in osteogenic αMEM 50% CXI extracts. (4) Conclusions: The fully injectable composite calcium phosphate bicements with anionic polyelectrolyte addition showed good mechanical and physico-chemical properties and enhanced osteogenic bioactivity which is a promising assumption for their application in bone defect regeneration.
Lubomir Medvecky; Radoslava Štulajterová; Maria Giretova; Lenka Luptakova; Tibor Sopčák. Injectable Enzymatically Hardened Calcium Phosphate Biocement. Journal of Functional Biomaterials 2020, 11, 74 .
AMA StyleLubomir Medvecky, Radoslava Štulajterová, Maria Giretova, Lenka Luptakova, Tibor Sopčák. Injectable Enzymatically Hardened Calcium Phosphate Biocement. Journal of Functional Biomaterials. 2020; 11 (4):74.
Chicago/Turabian StyleLubomir Medvecky; Radoslava Štulajterová; Maria Giretova; Lenka Luptakova; Tibor Sopčák. 2020. "Injectable Enzymatically Hardened Calcium Phosphate Biocement." Journal of Functional Biomaterials 11, no. 4: 74.
The phosphogypsum (PG) endogenous bacterial community and endophytic bacterial communities of four plants growing in phosphogypsum-contaminated sites, Suaeda fruticosa (SF), Suaeda mollis (SM), Mesembryanthmum nodiflorum (MN) and Arthrocnemum indicum (AI) were investigated by amplicon sequencing. Results highlight a more diverse community of phosphogypsum than plants associated endophytic communities. Additionally, the bacterial culturable communities of phosphogypsum and associated plant endophytes were isolated and their plant-growth promotion capabilities, bioremediation potential and stress tolerance studied. Most of plant endophytes were endowed with plant growth-promoting (PGP) activities and phosphogypsum communities and associated plants endophytes proved highly resistant to salt, metal and antibiotic stress. They also proved very active in bioremediation of phosphogypsum and other organic and inorganic environmental pollutants. Genome sequencing of five members of the phosphogypsum endogenous community showed that they belong to the recently described species Bacillus albus (BA). Genome mining of BA allowed the description of pollutant degradation and stress tolerance mechanisms. Prevalence of this tool box in the core, accessory and unique genome allowed to conclude that accessory and unique genomes are critical for the dynamics of strain acquisition of bioremediation abilities. Additionally, secondary metabolites (SM) active in bioremediation such as petrobactin have been characterized. Taken together, our results reveal hidden untapped valuable bacterial actors for waste remediation.
Fedia Ben Mefteh; Ali Chenari Bouket; Amal Daoud; Lenka Luptakova; Faizah N. Alenezi; Neji Gharsallah; Lassaad Belbahri; Ben Mefteh. Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation. Microorganisms 2019, 7, 382 .
AMA StyleFedia Ben Mefteh, Ali Chenari Bouket, Amal Daoud, Lenka Luptakova, Faizah N. Alenezi, Neji Gharsallah, Lassaad Belbahri, Ben Mefteh. Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation. Microorganisms. 2019; 7 (10):382.
Chicago/Turabian StyleFedia Ben Mefteh; Ali Chenari Bouket; Amal Daoud; Lenka Luptakova; Faizah N. Alenezi; Neji Gharsallah; Lassaad Belbahri; Ben Mefteh. 2019. "Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation." Microorganisms 7, no. 10: 382.
Oomycete and fungal pathogens, mainly Phytophthora and Fusarium species, are notorious causal agents of huge economic losses and environmental damages. For instance, Phytophthora ramorum, Phytophthora cryptogea, Phytophthora plurivora and Fusarium solani cause significant losses in nurseries and in forest ecosystems. Chemical treatments, while harmful to the environment and human health, have been proved to have little or no impact on these species. Recently, biocontrol bacterial species were used to cope with these pathogens and have shown promising prospects towards sustainable and eco-friendly agricultural practices. Olive trees prone to Phytophthora and Fusarium disease outbreaks are suitable for habitat-adapted symbiotic strategies, to recover oomycetes and fungal pathogen biocontrol agents. Using this strategy, we showed that olive trees-associated microbiome represents a valuable source for microorganisms, promoting plant growth and healthy benefits in addition to being biocontrol agents against oomycete and fungal diseases. Isolation, characterization and screening of root microbiome of olive trees against numerous Phytophthora and other fungal pathogens have led to the identification of the Bacillus velezensis OEE1, with plant growth promotion (PGP) abilities and strong activity against major oomycete and fungal pathogens. Phylogenomic analysis of the strain OEE1 showed that B. velezensis suffers taxonomic imprecision that blurs species delimitation, impacting their biofertilizers’ practical use. Genome mining of several B. velezensis strains available in the GenBank have highlighted a wide array of plant growth promoting rhizobacteria (PGPR) features, metals and antibiotics resistance and the degradation ability of phytotoxic aromatic compounds. Strain OEE1 harbours a large repertoire of secreted and volatile secondary metabolites. Rarefaction analysis of secondary metabolites richness in the B. velezenis genomes, unambiguously documented new secondary metabolites from ongoing genome sequencing efforts that warrants more efforts in order to assess the huge diversity in the species. Comparative genomics indicated that B. velezensis harbours a core genome endowed with PGP features and accessory genome encoding diverse secondary metabolites. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of OEE1 Volatile Organic Compounds (VOCs) and Liquid Chromatography High Resolution Mas Spectrometry (LC-HRMS) analysis of secondary metabolites identified numerous molecules with PGP abilities that are known to interfere with pathogen development. Moreover, B. velezensis OEE1 proved effective in protecting olive trees against F. solani in greenhouse experiments and are able to inhabit olive tree roots. Our strategy provides an effective means for isolation of biocontrol agents against recalcitrant pathogens. Their genomic analysis provides necessary clues towards their efficient implementation as biofertilizers.
Manel Cheffi; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Marta Belka; Armelle Vallat; Mostafa E. Rateb; Slim Tounsi; Mohamed Ali Triki; Lassaad Belbahri. Olea europaea L. Root Endophyte Bacillus velezensis OEE1 Counteracts Oomycete and Fungal Harmful Pathogens and Harbours a Large Repertoire of Secreted and Volatile Metabolites and Beneficial Functional Genes. Microorganisms 2019, 7, 314 .
AMA StyleManel Cheffi, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Marta Belka, Armelle Vallat, Mostafa E. Rateb, Slim Tounsi, Mohamed Ali Triki, Lassaad Belbahri. Olea europaea L. Root Endophyte Bacillus velezensis OEE1 Counteracts Oomycete and Fungal Harmful Pathogens and Harbours a Large Repertoire of Secreted and Volatile Metabolites and Beneficial Functional Genes. Microorganisms. 2019; 7 (9):314.
Chicago/Turabian StyleManel Cheffi; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Marta Belka; Armelle Vallat; Mostafa E. Rateb; Slim Tounsi; Mohamed Ali Triki; Lassaad Belbahri. 2019. "Olea europaea L. Root Endophyte Bacillus velezensis OEE1 Counteracts Oomycete and Fungal Harmful Pathogens and Harbours a Large Repertoire of Secreted and Volatile Metabolites and Beneficial Functional Genes." Microorganisms 7, no. 9: 314.
In the arid region Bou-Saâda at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Saâda, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5–10 pH range, and up to 30% polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0–400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.
Hafsa Cherif-Silini; Bathini Thissera; Ali Chenari Bouket; Nora Saadaoui; Allaoua Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Bilal Yahiaoui; Semcheddine Cherrad; Sebastien Vacher; Mostafa E. Rateb; Lassaad Belbahri. Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal. International Journal of Molecular Sciences 2019, 20, 3989 .
AMA StyleHafsa Cherif-Silini, Bathini Thissera, Ali Chenari Bouket, Nora Saadaoui, Allaoua Silini, Manal Eshelli, Faizah N. Alenezi, Armelle Vallat, Lenka Luptakova, Bilal Yahiaoui, Semcheddine Cherrad, Sebastien Vacher, Mostafa E. Rateb, Lassaad Belbahri. Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal. International Journal of Molecular Sciences. 2019; 20 (16):3989.
Chicago/Turabian StyleHafsa Cherif-Silini; Bathini Thissera; Ali Chenari Bouket; Nora Saadaoui; Allaoua Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Bilal Yahiaoui; Semcheddine Cherrad; Sebastien Vacher; Mostafa E. Rateb; Lassaad Belbahri. 2019. "Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal." International Journal of Molecular Sciences 20, no. 16: 3989.
Halophyte Limoniastrum monopetalum, an evergreen shrub inhabiting the Mediterranean region, has well-documented phytoremediation potential for metal removal from polluted sites. It is also considered to be a medicinal halophyte with potent activity against plant pathogens. Therefore, L. monopetalum may be a suitable candidate for isolating endophytic microbiota members that provide plant growth promotion (PGP) and resistance to abiotic stresses. Selected for biocontrol abilities, these endophytes may represent multifaceted and versatile biocontrol agents, combining pathogen biocontrol in addition to PGP and plant protection against abiotic stresses. In this study 117 root culturable bacterial endophytes, including Gram-positive (Bacillus and Brevibacillus), Gram-negative (Proteus, Providencia, Serratia, Pantoea, Klebsiella, Enterobacter and Pectobacterium) and actinomycete Nocardiopsis genera have been recovered from L. monopetalum. The collection exhibited high levels of biocontrol abilities against bacterial (Agrobacterium tumefaciens MAT2 and Pectobacterium carotovorum MAT3) and fungal (Alternaria alternata XSZJY-1, Rhizoctonia bataticola MAT1 and Fusarium oxysporum f. sp. radicis lycopersici FORL) pathogens. Several bacteria also showed PGP capacity and resistance to antibiotics and metals. A highly promising candidate Bacillus licheniformis LMRE 36 with high PGP, biocontrol, metal and antibiotic, resistance was subsequently tested in planta (potato and olive trees) for biocontrol of a collection of 14 highly damaging Fusarium species. LMRE 36 proved very effective against the collection in both species and against an emerging Fusarium sp. threatening olive trees culture in nurseries. These findings provide a demonstration of our pyramiding strategy. Our strategy was effective in combining desirable traits in biocontrol agents towards broad-spectrum resistance against pathogens and protection of crops from abiotic stresses. Stacking multiple desirable traits into a single biocontrol agent is achieved by first, careful selection of a host for endophytic microbiota recovery; second, stringent in vitro selection of candidates from the collection; and third, application of the selected biocontrol agents in planta experiments. That pyramiding strategy could be successfully used to mitigate effects of diverse biotic and abiotic stresses on plant growth and productivity. It is anticipated that the strategy will provide a new generation of biocontrol agents by targeting the microbiota of plants in hostile environments.
Houda Ben Slama; Mohamed Ali Triki; Ali Chenari Bouket; Fedia Ben Mefteh; Faizah N. Alenezi; Lenka Luptakova; Hafsa Cherif-Silini; Armelle Vallat; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents. Microorganisms 2019, 7, 249 .
AMA StyleHouda Ben Slama, Mohamed Ali Triki, Ali Chenari Bouket, Fedia Ben Mefteh, Faizah N. Alenezi, Lenka Luptakova, Hafsa Cherif-Silini, Armelle Vallat, Tomasz Oszako, Neji Gharsallah, Lassaad Belbahri. Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents. Microorganisms. 2019; 7 (8):249.
Chicago/Turabian StyleHouda Ben Slama; Mohamed Ali Triki; Ali Chenari Bouket; Fedia Ben Mefteh; Faizah N. Alenezi; Lenka Luptakova; Hafsa Cherif-Silini; Armelle Vallat; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. 2019. "Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents." Microorganisms 7, no. 8: 249.
To explore proteolytic activity of endophytic fungi inhabiting date palm roots, a Penicillium bilaiae isolate, displaying the highest level of protease production, has been recovered. Response surface methodology (RSM) was applied to optimize culture conditions for protease production by the fungus. Plackett-Burman design allowed for screening of variables effective in protease production. Results indicated that temperature, initial pH and glucose concentration dramatically affect protease yield. These factors were further optimized using a Box-Behnken design and RSM. A combination of initial pH (6.26), temperature (24.5 °C), glucose (13.75 g/L), NaNO₃ (1.5 g/L), MgSO₄ (0.2 g/L), KH₂PO₄ (0.5 g/L) and KCl (0.5 g/L) were optimum for maximum production of protease. A 1086-fold enhancement of protease production was gained after optimization. Biochemical properties of fungal protease including the effect of pH and temperature on the activity and the stability of proteolytic enzyme were determined. Moreover, the influence of carbon and nitrogen sources, metal ions, detergents as well as enzyme inhibitors was investigated. Our results highlighted that protease of Penicillium bilaiae isolate TDPEF30 could be considered as a promising candidate for industrial applications.
Fedia Ben Mefteh; Fakher Frikha; Amal Daoud; Ali Chenari Bouket; Lenka Luptakova; Faizah N. Alenezi; Bader S. Al-Anzi; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. Response Surface Methodology Optimization of an Acidic Protease Produced by Penicillium bilaiae Isolate TDPEF30, a Newly Recovered Endophytic Fungus from Healthy Roots of Date Palm Trees (Phoenix dactylifera L.). Microorganisms 2019, 7, 74 .
AMA StyleFedia Ben Mefteh, Fakher Frikha, Amal Daoud, Ali Chenari Bouket, Lenka Luptakova, Faizah N. Alenezi, Bader S. Al-Anzi, Tomasz Oszako, Neji Gharsallah, Lassaad Belbahri. Response Surface Methodology Optimization of an Acidic Protease Produced by Penicillium bilaiae Isolate TDPEF30, a Newly Recovered Endophytic Fungus from Healthy Roots of Date Palm Trees (Phoenix dactylifera L.). Microorganisms. 2019; 7 (3):74.
Chicago/Turabian StyleFedia Ben Mefteh; Fakher Frikha; Amal Daoud; Ali Chenari Bouket; Lenka Luptakova; Faizah N. Alenezi; Bader S. Al-Anzi; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. 2019. "Response Surface Methodology Optimization of an Acidic Protease Produced by Penicillium bilaiae Isolate TDPEF30, a Newly Recovered Endophytic Fungus from Healthy Roots of Date Palm Trees (Phoenix dactylifera L.)." Microorganisms 7, no. 3: 74.
Toxoplasma gondii is a worldwide parasite that is important both for veterinary medicine (economic losses in the herd) and for public health (immunocompromised patients and pregnant women). An important source of Toxoplasma infection in humans is consumption of contaminated meat and milk (undercooked meat and unpasteurized milk). Small ruminants are important in both milk and meat production throughout the world because of free-range husbandry. The purpose of our study was to detect the presence of T. gondii DNA in ewes' milk 1 month after the term, and to determine the relationship between the occurrence of this DNA in blood and milk based on the phase of infection. Using enzyme-linked immunosorbent assay (ELISA) the animals were divided into two groups (immunoglobulin M positive (IgM+), IgM-). With real-time polymerase chain reaction (PCR), T. gondii DNA was detected in seven milk samples (28%) and five blood samples (20%) of the IgM+ group (25 samples). In the IgM- group T. gondii DNA was detected in two milk samples (3.6%) out of 55 samples.
L. Luptakova; K. Benova; A. Rencko; E. Petrovova. DNA detection of Toxoplasma gondii in sheep milk and blood samples in relation to phase of infection. Veterinary Parasitology 2015, 208, 250 -253.
AMA StyleL. Luptakova, K. Benova, A. Rencko, E. Petrovova. DNA detection of Toxoplasma gondii in sheep milk and blood samples in relation to phase of infection. Veterinary Parasitology. 2015; 208 (3-4):250-253.
Chicago/Turabian StyleL. Luptakova; K. Benova; A. Rencko; E. Petrovova. 2015. "DNA detection of Toxoplasma gondii in sheep milk and blood samples in relation to phase of infection." Veterinary Parasitology 208, no. 3-4: 250-253.