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Natural dyes have been used from ancient times for multiple purposes, most importantly in the field of textile dying. The increasing demand and excessive costs of natural dye extraction engendered the discovery of synthetic dyes from petrochemical compounds. Nowadays, they are dominating the textile market, with nearly 8 × 105 tons produced per year due to their wide range of color pigments and consistent coloration. Textile industries consume huge amounts of water in the dyeing processes, making it hard to treat the enormous quantities of this hazardous wastewater. Thus, they have harmful impacts when discharged in non-treated or partially treated forms in the environment (air, soil, plants and water), causing several human diseases. In the present work we focused on synthetic dyes. We started by studying their classification which depended on the nature of the manufactured fiber (cellulose, protein and synthetic fiber dyes). Then, we mentioned the characteristics of synthetic dyes, however, we focused more on their negative impacts on the ecosystem (soil, plants, water and air) and on humans. Lastly, we discussed the applied physical, chemical and biological strategies solely or in combination for textile dye wastewater treatments. Additionally, we described the newly established nanotechnology which achieves complete discharge decontamination.
Houda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences 2021, 11, 6255 .
AMA StyleHouda Ben Slama, Ali Chenari Bouket, Zeinab Pourhassan, Faizah N. Alenezi, Allaoua Silini, Hafsa Cherif-Silini, Tomasz Oszako, Lenka Luptakova, Patrycja Golińska, Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences. 2021; 11 (14):6255.
Chicago/Turabian StyleHouda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. 2021. "Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods." Applied Sciences 11, no. 14: 6255.
Plant growth promoting bacteria (PGPB) have been the target of intensive research studies toward their efficient use in the field as biofertilizers, biocontrol, and bioremediation agents among numerous other applications. Recent trends in the field of PGPB research led to the development of versatile multifaceted PGPB that can be used in different field conditions such as biocontrol of plant pathogens in metal contaminated soils. Unfortunately, all these research efforts lead to the development of PGPB that failed to perform in salty environments. Therefore, it is urgently needed to address this drawback of these PGPB toward their efficient performance in salinity context. In this paper we provide a review of state-of-the-art research in the field of PGPB and propose a road map for the development of next generation versatile and multifaceted PGPB that can perform in salinity. Beyond soil desalinization, our study paves the way towards the development of PGPB able to provide services in diverse salty environments such as heavy metal contaminated, or pathogen threatened. Smart development of salinity adapted next generation biofertilizers will inevitably allow for mitigation and alleviation of biotic and abiotic threats to plant productivity in salty environments.
Hafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability 2021, 13, 4422 .
AMA StyleHafsa Cherif-Silini, Allaoua Silini, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Nawel Bouremani, Justyna Anna Nowakowska, Tomasz Oszako, Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability. 2021; 13 (8):4422.
Chicago/Turabian StyleHafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. 2021. "Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application." Sustainability 13, no. 8: 4422.
There is a great interest in mitigating soil salinity that limits plant growth and productivity. In this study, eighty-nine strains were isolated from the rhizosphere and endosphere of two halophyte species (Suaeda mollis and Salsola tetrandra) collected from three chotts in Algeria. They were screened for diverse plant growth-promoting traits, antifungal activity and tolerance to different physico-chemical conditions (pH, PEG, and NaCl) to evaluate their efficiency in mitigating salt stress and enhancing the growth of Arabidopsis thaliana and durum wheat under NaCl–stress conditions. Three bacterial strains BR5, OR15, and RB13 were finally selected and identified as Bacillus atropheus. The Bacterial strains (separately and combined) were then used for inoculating Arabidopsis thaliana and durum wheat during the seed germination stage under NaCl stress conditions. Results indicated that inoculation of both plant spp. with the bacterial strains separately or combined considerably improved the growth parameters. Three soils with different salinity levels (S1 = 0.48, S2 = 3.81, and S3 = 2.80 mS/cm) were used to investigate the effects of selected strains (BR5, OR15, and RB13; separately and combined) on several growth parameters of wheat plants. The inoculation (notably the multi-strain consortium) proved a better approach to increase the chlorophyll and carotenoid contents as compared to control plants. However, proline content, lipid peroxidation, and activities of antioxidant enzymes decreased after inoculation with the plant growth-promoting rhizobacteria (PGPR) that can attenuate the adverse effects of salt stress by reducing the reactive oxygen species (ROS) production. These results indicated that under saline soil conditions, halotolerant PGPR strains are promising candidates as biofertilizers under salt stress conditions.
Souhila Kerbab; Allaoua Silini; Ali Chenari Bouket; Hafsa Cherif-Silini; Manal Eshelli; Nour El Houda Rabhi; Lassaad Belbahri. Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria. Applied Sciences 2021, 11, 1034 .
AMA StyleSouhila Kerbab, Allaoua Silini, Ali Chenari Bouket, Hafsa Cherif-Silini, Manal Eshelli, Nour El Houda Rabhi, Lassaad Belbahri. Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria. Applied Sciences. 2021; 11 (3):1034.
Chicago/Turabian StyleSouhila Kerbab; Allaoua Silini; Ali Chenari Bouket; Hafsa Cherif-Silini; Manal Eshelli; Nour El Houda Rabhi; Lassaad Belbahri. 2021. "Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria." Applied Sciences 11, no. 3: 1034.
GO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction analysis (XRD), Raman spectroscopy, Thermo-gravimetric analysis (TGA), X-ray fluorescence (XRF), Zeta potential analysis, Scanning electron microscopy (SEM), and Atomic force microscopy (AFM) techniques were used to reveal that AgNPs have covered the surface of GO sheets through non covalent and permanent bonding, altering new structural and electronic properties, leading to the appearance of the oxidative stress phenomenon, which considered as a key step of the antibacterial mechanism of this kind of hybrids, causing death of both Gram-positive (Bacillus subtilis, Enterococcus faecalis, Methicillin-resistant Staphylococcus aureus - MRSA, and Staphylococcus aureus) and Gram-negative (Escherichia coli, Serratia marcescens, Shigella sp, Salmonella sp, Serratia microorganisms liquefaciens, Proteus sp, Enterobacter cloacae and Pseudomonas aeruginosa).
Samir Bouchareb; Rachida Doufnoune; Farid Riahi; Hafsa Cherif-Silini; Lassaad Belbahri. Non-covalent functionalization of graphene oxide using self-assembly of silver-triphenylphosphine for bactericidal formulations. Materials Chemistry and Physics 2019, 243, 122598 .
AMA StyleSamir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad Belbahri. Non-covalent functionalization of graphene oxide using self-assembly of silver-triphenylphosphine for bactericidal formulations. Materials Chemistry and Physics. 2019; 243 ():122598.
Chicago/Turabian StyleSamir Bouchareb; Rachida Doufnoune; Farid Riahi; Hafsa Cherif-Silini; Lassaad Belbahri. 2019. "Non-covalent functionalization of graphene oxide using self-assembly of silver-triphenylphosphine for bactericidal formulations." Materials Chemistry and Physics 243, no. : 122598.
In the arid region Bou-Saâda at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Saâda, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5–10 pH range, and up to 30% polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0–400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.
Hafsa Cherif-Silini; Bathini Thissera; Ali Chenari Bouket; Nora Saadaoui; Allaoua Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Bilal Yahiaoui; Semcheddine Cherrad; Sebastien Vacher; Mostafa E. Rateb; Lassaad Belbahri. Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal. International Journal of Molecular Sciences 2019, 20, 3989 .
AMA StyleHafsa Cherif-Silini, Bathini Thissera, Ali Chenari Bouket, Nora Saadaoui, Allaoua Silini, Manal Eshelli, Faizah N. Alenezi, Armelle Vallat, Lenka Luptakova, Bilal Yahiaoui, Semcheddine Cherrad, Sebastien Vacher, Mostafa E. Rateb, Lassaad Belbahri. Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal. International Journal of Molecular Sciences. 2019; 20 (16):3989.
Chicago/Turabian StyleHafsa Cherif-Silini; Bathini Thissera; Ali Chenari Bouket; Nora Saadaoui; Allaoua Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Bilal Yahiaoui; Semcheddine Cherrad; Sebastien Vacher; Mostafa E. Rateb; Lassaad Belbahri. 2019. "Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal." International Journal of Molecular Sciences 20, no. 16: 3989.
Halophyte Limoniastrum monopetalum, an evergreen shrub inhabiting the Mediterranean region, has well-documented phytoremediation potential for metal removal from polluted sites. It is also considered to be a medicinal halophyte with potent activity against plant pathogens. Therefore, L. monopetalum may be a suitable candidate for isolating endophytic microbiota members that provide plant growth promotion (PGP) and resistance to abiotic stresses. Selected for biocontrol abilities, these endophytes may represent multifaceted and versatile biocontrol agents, combining pathogen biocontrol in addition to PGP and plant protection against abiotic stresses. In this study 117 root culturable bacterial endophytes, including Gram-positive (Bacillus and Brevibacillus), Gram-negative (Proteus, Providencia, Serratia, Pantoea, Klebsiella, Enterobacter and Pectobacterium) and actinomycete Nocardiopsis genera have been recovered from L. monopetalum. The collection exhibited high levels of biocontrol abilities against bacterial (Agrobacterium tumefaciens MAT2 and Pectobacterium carotovorum MAT3) and fungal (Alternaria alternata XSZJY-1, Rhizoctonia bataticola MAT1 and Fusarium oxysporum f. sp. radicis lycopersici FORL) pathogens. Several bacteria also showed PGP capacity and resistance to antibiotics and metals. A highly promising candidate Bacillus licheniformis LMRE 36 with high PGP, biocontrol, metal and antibiotic, resistance was subsequently tested in planta (potato and olive trees) for biocontrol of a collection of 14 highly damaging Fusarium species. LMRE 36 proved very effective against the collection in both species and against an emerging Fusarium sp. threatening olive trees culture in nurseries. These findings provide a demonstration of our pyramiding strategy. Our strategy was effective in combining desirable traits in biocontrol agents towards broad-spectrum resistance against pathogens and protection of crops from abiotic stresses. Stacking multiple desirable traits into a single biocontrol agent is achieved by first, careful selection of a host for endophytic microbiota recovery; second, stringent in vitro selection of candidates from the collection; and third, application of the selected biocontrol agents in planta experiments. That pyramiding strategy could be successfully used to mitigate effects of diverse biotic and abiotic stresses on plant growth and productivity. It is anticipated that the strategy will provide a new generation of biocontrol agents by targeting the microbiota of plants in hostile environments.
Houda Ben Slama; Mohamed Ali Triki; Ali Chenari Bouket; Fedia Ben Mefteh; Faizah N. Alenezi; Lenka Luptakova; Hafsa Cherif-Silini; Armelle Vallat; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents. Microorganisms 2019, 7, 249 .
AMA StyleHouda Ben Slama, Mohamed Ali Triki, Ali Chenari Bouket, Fedia Ben Mefteh, Faizah N. Alenezi, Lenka Luptakova, Hafsa Cherif-Silini, Armelle Vallat, Tomasz Oszako, Neji Gharsallah, Lassaad Belbahri. Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents. Microorganisms. 2019; 7 (8):249.
Chicago/Turabian StyleHouda Ben Slama; Mohamed Ali Triki; Ali Chenari Bouket; Fedia Ben Mefteh; Faizah N. Alenezi; Lenka Luptakova; Hafsa Cherif-Silini; Armelle Vallat; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. 2019. "Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents." Microorganisms 7, no. 8: 249.
Stone surfaces are extreme environments that support microbial life. This microbial growth occurs despite unfavourable conditions associated with stone including limited sources of nutrients and water, high pH and exposure to extreme variations in temperature, humidity and irradiation. These stone-dwelling microbes are often resistant to extreme environments including exposure to desiccation, heavy metals, UV and Gamma irradiation. Here, we report on the effects of climate and stone geochemistry on microbiomes of Roman stone ruins in North Africa. Stone microbiomes were dominated by Actinobacteria, Cyanobacteria and Proteobacteria but were heavily impacted by climate variables that influenced water availability. Stone geochemistry also influenced community diversity, particularly through biologically available P, Mn and Zn. Functions associated with photosynthesis and UV protection were enriched in the metagenomes, indicating the significance of these functions for community survival on stones. Core members of the stone microbial communities were also identified and included Geodermatophilaceae, Rubrobacter, Sphingomonas and others. Our research has helped to expand the understanding of stone microbial community structure and functional capacity within the context of varying climates, geochemical properties and stone conditions.
Moussa Louati; Nathaniel J. Ennis; Faten Ghodhbane‐Gtari; Karima Hezbri; Joseph L. Sevigny; Maria F. Fahnestock; Hafsa Cherif-Silini; Julia G. Bryce; Louis S. Tisa; Maher Gtari. Elucidating the ecological networks in stone‐dwelling microbiomes. Environmental Microbiology 2019, 22, 1467 -1480.
AMA StyleMoussa Louati, Nathaniel J. Ennis, Faten Ghodhbane‐Gtari, Karima Hezbri, Joseph L. Sevigny, Maria F. Fahnestock, Hafsa Cherif-Silini, Julia G. Bryce, Louis S. Tisa, Maher Gtari. Elucidating the ecological networks in stone‐dwelling microbiomes. Environmental Microbiology. 2019; 22 (4):1467-1480.
Chicago/Turabian StyleMoussa Louati; Nathaniel J. Ennis; Faten Ghodhbane‐Gtari; Karima Hezbri; Joseph L. Sevigny; Maria F. Fahnestock; Hafsa Cherif-Silini; Julia G. Bryce; Louis S. Tisa; Maher Gtari. 2019. "Elucidating the ecological networks in stone‐dwelling microbiomes." Environmental Microbiology 22, no. 4: 1467-1480.
Date palm (Phoenix dactylifera L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by Fusarium oxysporum f. sp. albedinis, already responsible for destroying date production in other infected areas, mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, the additional benefits to agricultural ecosystems of using plant growth promoting rhizobacteria (PGPR) or endophytes are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization, meaning that it can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates, designated BFOA1, BFOA2, BFOA3 and BFOA4, that proved very active against F. oxysporum f. sp. albedinis. BFOA1–BFOA4 proved also active against 16 Fusarium isolates belonging to four species: F. oxysporum (with strains phytopathogenic of Olea europaea and tomato), F. solani (with different strains attacking O. europaea and potato), F. acuminatum (pathogenic on O. europaea) and F. chlamydosporum (phytopathogenic of O. europaea). BFOA1–BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens: Botrytis cinerea, Alternaria alternata, Phytophthora infestans, and Rhizoctonia bataticola. Isolates BFOA1–BFOA4 had the ability to grow at temperatures up to 35°C, pH range of 5–10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features, including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1–BFOA4 isolates indicated that they all belong to Bacillus halotolerans, which could therefore considered as a warden against Fusarium infection in plants. Comparative genomics allowed us to functionally describe the open pan genome of B. halotolerans and LC-HRMS and GCMS analyses, enabling the description of diverse secondary metabolites including pulegone, 2-undecanone, and germacrene D, with important antimicrobial and insecticidal properties. In conclusion, B. halotolerans could be used as an efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.
Houda Ben Slama; Hafsa Cherif-Silini; Ali Chenari Bouket; Mallique Qader; Allaoua Silini; Bilal Yahiaoui; Faizah N. Alenezi; Lenka Luptakova; Mohamed Ali Triki; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium. Frontiers in Microbiology 2019, 9, 3236 .
AMA StyleHouda Ben Slama, Hafsa Cherif-Silini, Ali Chenari Bouket, Mallique Qader, Allaoua Silini, Bilal Yahiaoui, Faizah N. Alenezi, Lenka Luptakova, Mohamed Ali Triki, Armelle Vallat, Tomasz Oszako, Mostafa E. Rateb, Lassaad Belbahri. Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium. Frontiers in Microbiology. 2019; 9 ():3236.
Chicago/Turabian StyleHouda Ben Slama; Hafsa Cherif-Silini; Ali Chenari Bouket; Mallique Qader; Allaoua Silini; Bilal Yahiaoui; Faizah N. Alenezi; Lenka Luptakova; Mohamed Ali Triki; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. 2019. "Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium." Frontiers in Microbiology 9, no. : 3236.
Aims The study aimed for evaluate the efficacy of Pseudomonas knackmussiiMLR6 on growth promotion, photosynthetic responses, pigment contents and gene expression of the plant model Arabidopsis thaliana under NaCl stress. Methods and Results The strain MLR6 was isolated from the rhizopshere of the halophyte Salsola tetrandra collected from a natural saline Algerian soil. Results showed the ability of MLR6 to induce plant growth‐promoting traits even under NaCl stress. The inoculation with MLR6 improved the stomatal conductance, the transpiration rate, the total chlorophyll and carotenoid contents under salt stress. It conferred also an increase in fresh/dry weight as well as plant height. MLR6 inoculation further provided a positive effect on cell membrane stability by reducing the electrolyte leakage and priming the ROS accumulation after the salt exposition. Additionally, the expression of NHX1, HKT1, SOS2 and SOS3 as well as SAG13 and PR1 was maintained in MLR6‐bacterized plant at a similar level of controls. Conclusions The inoculation of A. thaliana with MLR6 improves plant growth and reduces damages caused by salt stress. Significance and Impact of the Study The use of P. knackmussiiMLR6 appears as a promising strategy to improve the sustainable agriculture under saline conditions.
Nour El Houda Rabhi; Allaoua Silini; Hafsa Cherif-Silini; Bilal Yahiaoui; Abdelmalek Lekired; Mathilde Robineau; Qassim Esmaeel; Cédric Jacquard; Nathalie Vaillant-Gaveau; Christophe Clément; Essaïd Aït Barka; Lisa Sanchez. Pseudomonas knackmussiiMLR6, a rhizospheric strain isolated from halophyte, enhances salt tolerance inArabidopsis thaliana. Journal of Applied Microbiology 2018, 125, 1836 -1851.
AMA StyleNour El Houda Rabhi, Allaoua Silini, Hafsa Cherif-Silini, Bilal Yahiaoui, Abdelmalek Lekired, Mathilde Robineau, Qassim Esmaeel, Cédric Jacquard, Nathalie Vaillant-Gaveau, Christophe Clément, Essaïd Aït Barka, Lisa Sanchez. Pseudomonas knackmussiiMLR6, a rhizospheric strain isolated from halophyte, enhances salt tolerance inArabidopsis thaliana. Journal of Applied Microbiology. 2018; 125 (6):1836-1851.
Chicago/Turabian StyleNour El Houda Rabhi; Allaoua Silini; Hafsa Cherif-Silini; Bilal Yahiaoui; Abdelmalek Lekired; Mathilde Robineau; Qassim Esmaeel; Cédric Jacquard; Nathalie Vaillant-Gaveau; Christophe Clément; Essaïd Aït Barka; Lisa Sanchez. 2018. "Pseudomonas knackmussiiMLR6, a rhizospheric strain isolated from halophyte, enhances salt tolerance inArabidopsis thaliana." Journal of Applied Microbiology 125, no. 6: 1836-1851.
Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-β-picrylhydrazyl (DPPH), β-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography–High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography–Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.
Fedia Ben Mefteh; Amal Daoud; Ali Chenari Bouket; Bathini Thissera; Yamina Kadri; Hafsa Cherif-Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Tomasz Oszako; Adel Kadri; José María Ros-García; Mostafa E. Rateb; Neji Gharsallah; Lassaad Belbahri. Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites. International Journal of Molecular Sciences 2018, 19, 1986 .
AMA StyleFedia Ben Mefteh, Amal Daoud, Ali Chenari Bouket, Bathini Thissera, Yamina Kadri, Hafsa Cherif-Silini, Manal Eshelli, Faizah N. Alenezi, Armelle Vallat, Tomasz Oszako, Adel Kadri, José María Ros-García, Mostafa E. Rateb, Neji Gharsallah, Lassaad Belbahri. Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites. International Journal of Molecular Sciences. 2018; 19 (7):1986.
Chicago/Turabian StyleFedia Ben Mefteh; Amal Daoud; Ali Chenari Bouket; Bathini Thissera; Yamina Kadri; Hafsa Cherif-Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Tomasz Oszako; Adel Kadri; José María Ros-García; Mostafa E. Rateb; Neji Gharsallah; Lassaad Belbahri. 2018. "Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites." International Journal of Molecular Sciences 19, no. 7: 1986.
This study was conducted to determine the effect of plant growth promoting rhizobacteria Azotobacter chroococcum AZ6 isolated from arid soil and osmolytes such as glycine betaine (GB) or proline (P) on the growth of durum wheat varieties under salinity stress. Inoculation by A. chroococcum AZ6 in the presence or absence of P (5 mM) or GB (5 mM) reduced substantially the effect of salt stress on plant growth parameters such as root length, plant height, fresh shoot and root weight and dry shoot and root weight. The differences between the two varieties were low but with a fresh and dry weight higher in Waha. The rate of Na+ accumulation in the roots and the shoots was important up to 100 mM and increased at 200 mM. The K+ concentration and chlorophyll content decreased but proline and amino acid contents were enhanced with increasing salinity. Treatment by inoculation in the presence or absence of osmolytes improved the chlorophyll (a and total) and the K+ concentrations and reduced intracellular proline accumulation and amino acids contents. Also, as result, the use of A. chroococcum AZ6 and osmolytes treatment may provide a means of improving tolerance of durum wheat to salt stress. Key words: Durum wheat, salinity, osmolytes, Azotobacter chroococcum,
Silini Allaoua; Cherif Silini Hafsa; Yahiaoui Bilal; Allaoua Silini; Hafsa Cherif-Silini; Bilal Yahiaoui. Growing varieties durum wheat (Triticum durum) in response to the effect of osmolytes and inoculation by Azotobacter chroococcum under salt stress. African Journal of Microbiology Research 2016, 10, 387 -399.
AMA StyleSilini Allaoua, Cherif Silini Hafsa, Yahiaoui Bilal, Allaoua Silini, Hafsa Cherif-Silini, Bilal Yahiaoui. Growing varieties durum wheat (Triticum durum) in response to the effect of osmolytes and inoculation by Azotobacter chroococcum under salt stress. African Journal of Microbiology Research. 2016; 10 (12):387-399.
Chicago/Turabian StyleSilini Allaoua; Cherif Silini Hafsa; Yahiaoui Bilal; Allaoua Silini; Hafsa Cherif-Silini; Bilal Yahiaoui. 2016. "Growing varieties durum wheat (Triticum durum) in response to the effect of osmolytes and inoculation by Azotobacter chroococcum under salt stress." African Journal of Microbiology Research 10, no. 12: 387-399.
The rhizobacteria that promote the growth of plants can have a positive effect on the productivity of crops, especially in stress conditions. Among the plant -growth -promoting (PGP) rhizobacteria (PGPR) cluster, Bacillus spp. are among the genera with most potential due to their spore forming ability, thereby increasing the adaptation of Bacillus strains to commercial formulation and field application. Due to their intrinsic properties, the Bacilli have several mechanisms conferring beneficial effects on plants. Thirty-five strains of Bacillus isolated from the rhizosphere of wheat from three different soils in arid and semi-arid areas of Algeria were tested for properties involved in the promotion of plant growth. The PGP ability of the 35 strains was evaluated by determining their biofertilisation (phosphate solubilisation), biostimulation [indole acetic acid (IAA) production] and biocontrol [cyanhydric acid (HCN), siderophores, 2,3-butanediol production and antifungal activity] activities. Of the 35 strains, 78 % had the ability to solubilise phosphates at rates of 16.65 μg/mL for strain D13, 15.60 μg/mL for D7 and 15.05 μg/mL for D6. These strains were the most successful and were isolated from arid and alkaline soils. The highest concentrations of IAA were produced by strains D4 and D7 to values ranging from 10 to 19 μg/mL. All strains inhibited at least one fungal strain tested, and 75 % had activity against three fungi or more. More than half of Bacillus strains produced 2.3- butanediol but only a single strain produced HCN. Only three strains (B25, D11 and BA11) were efficient in the production of siderophores. Also, four strains (B21, D4, B10 and B25) possessed ACCdeaminase and were considered regulators of stress. Phylogenetic diversity of the strains was analysed by 16S rDNA sequencing. The results identified all strains as being similar to the Bacillus sp. cluster, and divided separately into five groups. The majority of strains (n = 28) were assigned to the species Bacillus thuringiensis and Bacillus subtilis. The Bacillus species isolated in this study showing PGP abilities have the potential to be used as PGPR.
Hafsa Cherif-Silini; Allaoua Silini; Bilal Yahiaoui; Imen Ouzari; Abdellatif Boudabous. Phylogenetic and plant-growth-promoting characteristics of Bacillus isolated from the wheat rhizosphere. Annals of Microbiology 2016, 66, 1087 -1097.
AMA StyleHafsa Cherif-Silini, Allaoua Silini, Bilal Yahiaoui, Imen Ouzari, Abdellatif Boudabous. Phylogenetic and plant-growth-promoting characteristics of Bacillus isolated from the wheat rhizosphere. Annals of Microbiology. 2016; 66 (3):1087-1097.
Chicago/Turabian StyleHafsa Cherif-Silini; Allaoua Silini; Bilal Yahiaoui; Imen Ouzari; Abdellatif Boudabous. 2016. "Phylogenetic and plant-growth-promoting characteristics of Bacillus isolated from the wheat rhizosphere." Annals of Microbiology 66, no. 3: 1087-1097.
The Bacillus by their intrinsic properties have several mechanisms of procuring beneficial effects to plants. They are among the most successful bacterial communities in the solubilization of phosphates. Ten Bacillus strains were isolated from the rhizosphere of wheat from three areas in arid and semi-arid regions in Algeria. These strains were tested for their ability to solubilize phosphates on liquid and solid media: LB (Luria-Bertani), NBRIP (National Botanical Research Institute Phosphate) and PVK (Pikovskaya) supplemented with Ca3(PO4)2 (5 g/l). The highest levels of soluble phosphates were obtained on PVK and NBRIP liquid (177.68 and 173.28 µg/ml for D13 and D1 strains, respectively). Phosphates solubilization is always associated with a decrease in the pH of the medium. An inverse relationship (r = -0.4224) existed between the levels of soluble phosphate and the decrease of pH in medium. To test the solubilization capacity of theBacillus strains under salt stress, concentrations of NaCl (0, 2, 4, 6 and 8%) were added to the NBRIP medium. The solubilization capacity was negatively affected by the increase of salinity. However, the response of the Bacillus to salt was strain dependent. Strains D1 and D13, which produced the best rate of solubilization were used to study the ability of osmoprotectants (glycine-betaine, proline) to restore the ability to solubilize phosphate at high salt concentrations. However, these strains do not seem to accumulate these types of osmoprotectants. Key words: Bacillus, phosphate solubilization, NaCl, osmoprotectant.
Cherif Silini Hafsa; Silini Allaoua; Ghoul Mostefa; Yahiaoui Bilal; Arif Fouzia; Hafsa Cherif-Silini; Allaoua Silini; Mostefa Ghoul; Bilal Yahiaoui; Fouzia Arif. Solubilization of phosphate by the Bacillus under salt stress and in the presence of osmoprotectant compounds. African Journal of Microbiology Research 2013, 7, 4562 -4571.
AMA StyleCherif Silini Hafsa, Silini Allaoua, Ghoul Mostefa, Yahiaoui Bilal, Arif Fouzia, Hafsa Cherif-Silini, Allaoua Silini, Mostefa Ghoul, Bilal Yahiaoui, Fouzia Arif. Solubilization of phosphate by the Bacillus under salt stress and in the presence of osmoprotectant compounds. African Journal of Microbiology Research. 2013; 7 (37):4562-4571.
Chicago/Turabian StyleCherif Silini Hafsa; Silini Allaoua; Ghoul Mostefa; Yahiaoui Bilal; Arif Fouzia; Hafsa Cherif-Silini; Allaoua Silini; Mostefa Ghoul; Bilal Yahiaoui; Fouzia Arif. 2013. "Solubilization of phosphate by the Bacillus under salt stress and in the presence of osmoprotectant compounds." African Journal of Microbiology Research 7, no. 37: 4562-4571.
The use of microbial technology in agriculture is expanding quickly with the identification of new bacterial strains which are more effective in promoting the growth of plants. The rhizobacteria that promote the growth of plants can have a positive effect on the productivity of crops especially when subjected to salt stress. A nitrogen-fixing bacterium was isolated from the wheat rhizosphere of an arid region. The strain was identified on the basis of tests API20E and 16S rRNA sequencing, as Pantoea agglomerans lma2. This strain degraded several carbon sources: sugars (fructose, ribose, dextrin, salicin...), lipids (lecithin, tributyrin and tween 80), proteins (gelatin, casein), grew on KCN and could grow from pH 4 to 8 and had an optimum at pH 7. The growth temperature showed a maximum at 30 degrees C and the bacteria could tolerate from 4 to 41 degrees C and the growth rate was higher when the NaCl concentration was between 100 and 300 mM. The performance of activities enhancing the growth of plants of P. agglomerans lma2 was significantly better in the presence of salt. Rates of Indole Acetic Acid (IAA), siderophores production and solubilization of phosphate increased between 100 and 400 mM NaCl compared to the control without salt. The maximum values were saved to 300 mM for the production of siderophores (18.32%) and solubilization of phosphate (1061.49 microg mL(-1)) and 100 mM for the production of IAA (161 microg mL(-1)). A significant correlation existed between these three activities. These results showed that P. agglomerans lma2 with its Plant Growth Promoting Rhizobacteria (PGPR) and halophilic properties could constitute a good fertilizer in arid and saline zone.
H. Silini-Che; Hafsa Cherif-Silini; M. Ghoul; S. Yadav. Isolation and Characterization of Plant Growth Promoting Traits of a Rhizobacteria: Pantoea agglomerans lma2. Pakistan Journal of Biological Sciences 2012, 15, 267 -276.
AMA StyleH. Silini-Che, Hafsa Cherif-Silini, M. Ghoul, S. Yadav. Isolation and Characterization of Plant Growth Promoting Traits of a Rhizobacteria: Pantoea agglomerans lma2. Pakistan Journal of Biological Sciences. 2012; 15 (6):267-276.
Chicago/Turabian StyleH. Silini-Che; Hafsa Cherif-Silini; M. Ghoul; S. Yadav. 2012. "Isolation and Characterization of Plant Growth Promoting Traits of a Rhizobacteria: Pantoea agglomerans lma2." Pakistan Journal of Biological Sciences 15, no. 6: 267-276.
The effect of plant growth-promoting Rhizobacteria (PGPR) and exogenous application of compatible solutes on seed germination and root concentrations of sodium and potassium of two wheat varieties (Triticum durum L.) were evaluated under saline stress. In this experiment, Azotobacter vinelandii strain DSM85, glycine betaine and proline were used. Inoculated seeds for each variety were placed on Whatman paper in 9 cm Petri dishes containing 15 mL of distilled water or NaCl solutions at various concentrations (control, 100, 200, 300 mM) supplemented with or without glycine betaine (GB) or proline at 5 mM. The results indicated that addition of proline (5 mM) stimulated the production of indol acetic acid and the growth of A. vinelandii at 200 and 300 mM NaCl, respectively. The germination rate index and the germination final percentage decreased significantly (p < 0.05) with increasing salinity level. The germination was significantly diminished at 300 mM with significant variation among varieties and Waha variety had higher germination percentage than Bousselam variety. Inoculation of seeds by A. vinelandii and exogenous application of proline had significantly positive effect on the germination at this concentration of NaCl. The rate of accumulation of Na+ in roots was important at 100 mM and increased at 200 mM. The concentration of K+ decreased when salinity increased. The effect of inoculation or inoculation with proline decreased the accumulation of Na' and reduced the loss of K+ under salt stress. From the present study we can conclude that the use of A. vinelandii strain DSM85 and external application of low concentrations of proline on seeds might be considered as a strategy for the protection of plants under saline stress.
Hafsa Cherif-Silini; H. Silini-Che; M. Ghoul. Effect of Azotobacter vinelandii and Compatible Solutes on Germination Wheat Seeds and Root Concentrations of Sodium and Potassium under Salt Stress. Pakistan Journal of Biological Sciences 2012, 15, 132 -140.
AMA StyleHafsa Cherif-Silini, H. Silini-Che, M. Ghoul. Effect of Azotobacter vinelandii and Compatible Solutes on Germination Wheat Seeds and Root Concentrations of Sodium and Potassium under Salt Stress. Pakistan Journal of Biological Sciences. 2012; 15 (3):132-140.
Chicago/Turabian StyleHafsa Cherif-Silini; H. Silini-Che; M. Ghoul. 2012. "Effect of Azotobacter vinelandii and Compatible Solutes on Germination Wheat Seeds and Root Concentrations of Sodium and Potassium under Salt Stress." Pakistan Journal of Biological Sciences 15, no. 3: 132-140.
The hydrodistilled oils from the aerial parts of Genista ulicina Spach. and G. vepres Pomel., which are endemic to Algeria, were analyzed by gas chromatography-mass spectrometry (GC-MS). In the oil of G. ulicina, 41 compounds were identified representing 90.8% of the total oil, and in G. vepres, 61 compounds representing 84.5% of the total oil. The analyses showed that the major constituents of the oils were lauric acid (14.3% – 8.5%), myristic acid (11.5% – 5%), linoleic acid (3.1% –11.7%) and palmitic acid (18.6% – 26.4%). Using a diffusion method, the oils showed significant antibacterial activities against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923).
Takia Lograda; Adel Nadjib Chaker; Jean Claude Chalchat; Messaoud Ramdani; Hafsa Cherif-Silini; Gilles Figueredo; Pierre Chalard. Chemical Composition and Antimicrobial Activity of Essential Oils of Genista ulicina and G. vepres. Natural Product Communications 2010, 5, 1 .
AMA StyleTakia Lograda, Adel Nadjib Chaker, Jean Claude Chalchat, Messaoud Ramdani, Hafsa Cherif-Silini, Gilles Figueredo, Pierre Chalard. Chemical Composition and Antimicrobial Activity of Essential Oils of Genista ulicina and G. vepres. Natural Product Communications. 2010; 5 (5):1.
Chicago/Turabian StyleTakia Lograda; Adel Nadjib Chaker; Jean Claude Chalchat; Messaoud Ramdani; Hafsa Cherif-Silini; Gilles Figueredo; Pierre Chalard. 2010. "Chemical Composition and Antimicrobial Activity of Essential Oils of Genista ulicina and G. vepres." Natural Product Communications 5, no. 5: 1.
T. Lograda; A. N. Chaker; P. Chalard; M. Ramdani; J. C. Chalchat; Hafsa Cherif-Silini; G. Figueredo. Chemical Composition and Antimicrobial Activity of Essential oil of Genista numidica Spach. and G. saharae Coss et Dur. Asian Journal of Plant Sciences 2009, 8, 495 -499.
AMA StyleT. Lograda, A. N. Chaker, P. Chalard, M. Ramdani, J. C. Chalchat, Hafsa Cherif-Silini, G. Figueredo. Chemical Composition and Antimicrobial Activity of Essential oil of Genista numidica Spach. and G. saharae Coss et Dur. Asian Journal of Plant Sciences. 2009; 8 (7):495-499.
Chicago/Turabian StyleT. Lograda; A. N. Chaker; P. Chalard; M. Ramdani; J. C. Chalchat; Hafsa Cherif-Silini; G. Figueredo. 2009. "Chemical Composition and Antimicrobial Activity of Essential oil of Genista numidica Spach. and G. saharae Coss et Dur." Asian Journal of Plant Sciences 8, no. 7: 495-499.