Bovine respiratory disease (BRD) is a huge economic burden on the livestock industries of countries worldwide. Bovine respiratory syncytial virus (BRSV) is one of the most important pathogens that contributes to BRD. In this study, we report the identification and first isolation, with molecular characterization, of a new BRSV strain from lung specimens of three beef cows in Turkey that died from respiratory distress. After the screening of lung tissues for BRD-associated viruses using a multiscreen antigen-ELISA, a BRSV antigen was detected. This was then confirmed by real-time RT-PCR specific for BRSV. Following confirmation, virus isolation was conducted in MDBK cell cultures and clear CPE, including syncytia compatible with BRSV, were detected. RT-nested PCR, using F gene-specific primers, was performed on the cultured isolates, and the products were sequenced and deposited to Genbank with accession numbers MT179304, MT024766, and MT0244767. Phylogenetic analysis of these sequences indicated that the cattle were infected with BRSV from subgroup III and were closely related to previously identified American and Turkish strains, but contained some amino acid and nucleotide differences. This research paves the way for further studies on the molecular characteristics of natural BRSV isolates, including full genome analysis and disease pathogenesis, and also contributes to the development of robust national strategies against this virus.

Maedi-Visna is a slowly progressive infection of sheep that affects the respiratory and nervous systems and causes significant yield losses worldwide. This study was conducted to assess the seroprevalence of Maedi-Visna infection in sheep across several provinces in the Central Black Sea region of Türkiye, namely Samsun, Sinop, Ordu, Giresun, Amasya and Tokat. A total of 476 sheep serum samples were collected for further analysis. The presence of antibodies in the sera was determined by Ab ELISA. As a result of the study, 263 (55.25%) blood serum samples were positive for the presence of MVV antibodies. Seropositivity rates by province were as follows: Samsun 45%, Sinop 80%, Ordu 58.75%, Giresun 35%, Amasya 38.75%, Tokat 75%. Notably, our findings suggest a significantly high seroprevalence of Maedi-Visna infection in this region compared to other studies conducted in different parts of Türkiye This information is pivotal for understanding the extent of the infection in the sheep population of the Central Black Sea region and can contribute to the development of strategies for disease management and control.

Mavidil virusu (MDV) Avrupa’da önemli verim kayıplarına sebep olan vektörlerle nakledilen ve özellikle koyunlarda teratojenik etkiye sahip bir virustur. Bu çalışma koyunlarda Orta Karadeniz Bölgesinde yer alan Samsun, Sinop, Ordu, Giresun ve Amasya illerinde MDV’nin seroprevalansını araştırmak amacıyla yapılmıştır. Her bir ilden 80 adet olmak üzere toplam 400 adet koyundan serum örnekleri toplandı. MDV’ye spesifik antikor varlığını belirlemek için antikor (Ab) ELISA test tekniği kullanıldı. Test sonucunda 10 koyunun (%2,5) MDV (Ab) yönünden pozitif olduğu tespit edildi. Amasya’da %2,5, Sinop’ta %10 oranında seropozitiflik tespit edilirken Samsun, Ordu ve Giresun illerinde seropozitiflik belirlenmedi. Elde edilen veriler MDV enfeksiyonun bölgede düşük seroprevalansa sahip olduğunu göstermektedir. Etkene karşı aşılama yapılmayan Orta Karadeniz Bölgesinde seropozitifliğin tespit edilmesi bölgede aşılama çalışmalarının gerekliliğini göstermektedir.