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Infectious pancreatic necrosis (IPN) is a highly contagious disease causing high mortality in juvenile trouts. Since there is no effective way to treatment against IPNV, early diagnosis and prevention play an important role in combating the disease. The different types of IPNV vaccines (inactive, live, recombinant, DNA, etc) have been produced from local isolates and have been used in developed countries. In Turkey, there is no commercial licensed vaccines against IPNV. Due to this reason, IPNV vaccine is needed in Turkey. The production of recombinant VP2 subunit vaccine (IPNV-VP2) and inactivated whole particle virus vaccine (IPNV-WPV) were attempted from selected isolate belong to sp serotype. For this purpose; the virus was produced in RTG-2 cell line and RT-PCR amplification was performed by using primers with restriction enzymes. The whole VP2 gene was cloned into a plasmid vector and VP2 was expressed by using E. coli expression system. A trial was conducted to determine the immunity ability of IPNV-VP2 and IPNV-WPV in rainbow trout. According to the SN50 assay, the IPNV-WPV stimulates immune response faster than the IPNV-VP2 vaccine. Besides, the relative percent of Survive (RPS) was detected as 79% in fish vaccinated with IPNV-WPV and 70% in fish vaccinated with IPNV-VP2. Thus, we can say that the recombinant vaccine of IPNV-VP2 is almost protected against IPNV infection as well as the inactive vaccine.
Cuneyt Tamer; Abdullah Cavunt; Yuksel Durmaz; Emre Ozan; Hamza Kadi; Gulnur Kalayci; Buket Ozkan; Hakan Isidan; Harun Albayrak. Inactivated infectious pancreatic necrosis virus (IPNV) vaccine and E.coli-expressed recombinant IPNV-VP2 subunit vaccine afford protection against IPNV challenge in rainbow trout. Fish & Shellfish Immunology 2021, 115, 205 -211.
AMA StyleCuneyt Tamer, Abdullah Cavunt, Yuksel Durmaz, Emre Ozan, Hamza Kadi, Gulnur Kalayci, Buket Ozkan, Hakan Isidan, Harun Albayrak. Inactivated infectious pancreatic necrosis virus (IPNV) vaccine and E.coli-expressed recombinant IPNV-VP2 subunit vaccine afford protection against IPNV challenge in rainbow trout. Fish & Shellfish Immunology. 2021; 115 ():205-211.
Chicago/Turabian StyleCuneyt Tamer; Abdullah Cavunt; Yuksel Durmaz; Emre Ozan; Hamza Kadi; Gulnur Kalayci; Buket Ozkan; Hakan Isidan; Harun Albayrak. 2021. "Inactivated infectious pancreatic necrosis virus (IPNV) vaccine and E.coli-expressed recombinant IPNV-VP2 subunit vaccine afford protection against IPNV challenge in rainbow trout." Fish & Shellfish Immunology 115, no. : 205-211.
The aquatic virus, infectious pancreatic necrosis virus (IPNV), is known to infect various farmed fish, in particular salmonids, and is responsible for large economic losses in the aquaculture industry. Common practices to detect the virus include qPCR tests based on specific primers and serum neutralization tests for virus serotyping. Following the potential presence of IPNV viruses in a fish farm in Scotland containing vaccinated and IPNV-resistant fish, the common serotyping of the IPNV isolates was not made possible. This led us to determine the complete genome of the new IPNV isolates in order to investigate the cause of the serotyping discrepancy. Next-generation sequencing using the Illumina technology along with the sequence-independent single primer amplification (SISPA) approach was conducted to fully characterize the new Scottish isolates. With this approach, the full genome of two isolates, V1810–4 and V1810–6, was determined and analyzed. The potential origin of the virus isolates was investigated by phylogenetic analyses along with tridimensional and secondary protein structure analyses. These revealed the emergence of a new variant from one of the main virus serotypes, probably caused by the presence of selective pressure exerted by the vaccinated IPNV-resistant farmed fish.
Jessica Benkaroun; Katherine Muir; Rosa Allshire; Cüneyt Tamer; Manfred Weidmann. Isolation of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant from a Fish Farm in Scotland. Viruses 2021, 13, 385 .
AMA StyleJessica Benkaroun, Katherine Muir, Rosa Allshire, Cüneyt Tamer, Manfred Weidmann. Isolation of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant from a Fish Farm in Scotland. Viruses. 2021; 13 (3):385.
Chicago/Turabian StyleJessica Benkaroun; Katherine Muir; Rosa Allshire; Cüneyt Tamer; Manfred Weidmann. 2021. "Isolation of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant from a Fish Farm in Scotland." Viruses 13, no. 3: 385.
Viral haemorrhagic septicaemia (VHS) is a serious disease of finfish with a high mortality rate (70%) worldwide. It is caused by infectious viral haemorrhagic septicaemia virus (VHSV). No commercial vaccine is available against VHSV so far. In the present study, we aimed to develop a sustainable recombinant subunit vaccine against a local isolate of VHSV belonging to the genogroup Ie. In order to develop a recombinant vaccine against VHSV genogroup Ie, a cDNA construct of the VHSV gG gene was amplified by reverse transcriptase‐polymerase chain reaction (RT‐PCR) and cloned into an Escherichia coli expression vector (pET‐28a) to express recombinant protein products. In addition, a formol‐inactivated whole particle vaccine was prepared. Subsequently, rainbow trout (17–60 g) were vaccinated by injecting purified recombinant VHSV gG proteins with an adjuvant (montanide) and the formol‐inactivated whole particle vaccine and then challenged with moderately virulent VHSV. At the end of the trial, the mortality rate was found to be 10% (RPS 33.3%) in the recombinant vaccine group, while no mortality was detected (RPS 100%) in the formol‐inactivated whole particle vaccine group. The recombinant subunit vaccine did not provide as much protection as the inactivated whole particle vaccine, even if the desired antibody production and viral load decreased.
Cuneyt Tamer; Harun Albayrak; Semra Gumusova. Comparison of immune response between Escherichia coli ‐derived recombinant subunit vaccine and formol‐inactivated whole particle vaccine against viral haemorrhagic septicaemia virus (VHSV) in rainbow trout. Aquaculture Research 2021, 52, 2706 -2714.
AMA StyleCuneyt Tamer, Harun Albayrak, Semra Gumusova. Comparison of immune response between Escherichia coli ‐derived recombinant subunit vaccine and formol‐inactivated whole particle vaccine against viral haemorrhagic septicaemia virus (VHSV) in rainbow trout. Aquaculture Research. 2021; 52 (6):2706-2714.
Chicago/Turabian StyleCuneyt Tamer; Harun Albayrak; Semra Gumusova. 2021. "Comparison of immune response between Escherichia coli ‐derived recombinant subunit vaccine and formol‐inactivated whole particle vaccine against viral haemorrhagic septicaemia virus (VHSV) in rainbow trout." Aquaculture Research 52, no. 6: 2706-2714.
Marek's disease (MD) is an important disease of avian species and a potential threat to the poultry industry worldwide. In this study, 16 dead commercial chickens from flocks with suspected MD were necropsied immediately after death. Pathological findings were compatible with MD, and gallid alphaherpesvirus 2 was identified in PCR of spleen samples. Virus isolation was performed in primary cell culture, and partial sequencing of the meq gene of the isolate revealed >99% nucleotide sequence identity to virulent and very virulent plus strains from a number of European countries, placing it in the same subclade of clade III as two virulent Italian strains and a very virulent plus Polish strain as well as virulent strains of geese and ducks. The data reported here indicate that a virulent strain of Marek’s disease virus is circulating in Turkey and has not been stopped by the current national vaccination programme.
Emre Ozan; Bahadir Muftuoglu; Ismail Sahindokuyucu; Hanne Nur Kurucay; Sinem Inal; Nilufer Kuruca; Ahmed Eisa Elhag; Efe Karaca; Cuneyt Tamer; Semra Gumusova; Harun Albayrak; Gerald Barry; Mustafa Yavuz Gulbahar; Zafer Yazici. Marek’s disease virus in vaccinated poultry flocks in Turkey: its first isolation with molecular characterization. Archives of Virology 2021, 166, 559 -569.
AMA StyleEmre Ozan, Bahadir Muftuoglu, Ismail Sahindokuyucu, Hanne Nur Kurucay, Sinem Inal, Nilufer Kuruca, Ahmed Eisa Elhag, Efe Karaca, Cuneyt Tamer, Semra Gumusova, Harun Albayrak, Gerald Barry, Mustafa Yavuz Gulbahar, Zafer Yazici. Marek’s disease virus in vaccinated poultry flocks in Turkey: its first isolation with molecular characterization. Archives of Virology. 2021; 166 (2):559-569.
Chicago/Turabian StyleEmre Ozan; Bahadir Muftuoglu; Ismail Sahindokuyucu; Hanne Nur Kurucay; Sinem Inal; Nilufer Kuruca; Ahmed Eisa Elhag; Efe Karaca; Cuneyt Tamer; Semra Gumusova; Harun Albayrak; Gerald Barry; Mustafa Yavuz Gulbahar; Zafer Yazici. 2021. "Marek’s disease virus in vaccinated poultry flocks in Turkey: its first isolation with molecular characterization." Archives of Virology 166, no. 2: 559-569.
Infectious pancreatic necrosis virus (IPNV) and viral haemorrhagic septicaemia virus (VHSV) are two of the major viral threats faced by the aquaculture industry in Turkey. The aim of our study was to investigate the pathogenicity of two Turkish viral strains isolated locally from the Bolu VHSV strain (Accession number: KM972678.1) and the HAH‐4 IPNV strain (Accession number: KM972675). The titres of infectious virus were determined by virus titration tests using monolayer cultures of EPC cells to determine the challenge dose. The challenge trial was conducted with 40 rainbow trout (Oncorhynchus mykiss (Walbaum)) for each virus and control group. The infective dose of each virus was applied intraperitoneally as 1 × 107 of the tissue culture infective dose per ml. At the end of the trial period (day 21), all fish were examined for clinical signs and post‐mortem changes. The average mortality rates for VHSV and IPNV were 36.6% and 33.3%, respectively. Necropsies performed on the deceased fish revealed the presence of IPNV only in fish that had been infected with IPNV, as determined using a real‐time PCR method targeting the VP3 gene region of the virus. Similarly, VHSV was detected only in the fish infected with VHSV using a real‐time PCR method targeting the gG gene region of the virus. In conclusion, the Bolu strain of VHSV and the HAH‐4 strain of IPNV each has moderate pathogenicity in rainbow trout.
Cuneyt Tamer; Yuksel Durmaz; Emre Ozan; Hamza Kadi; Abdullah Cavunt; Bahadir Muftuoglu; Harun Albayrak. Pathogenicity trials regarding Turkish isolates of infectious pancreatic necrosis virus and viral haemorrhagic septicaemia virus in rainbow trout. Aquaculture Research 2020, 52, 1395 -1400.
AMA StyleCuneyt Tamer, Yuksel Durmaz, Emre Ozan, Hamza Kadi, Abdullah Cavunt, Bahadir Muftuoglu, Harun Albayrak. Pathogenicity trials regarding Turkish isolates of infectious pancreatic necrosis virus and viral haemorrhagic septicaemia virus in rainbow trout. Aquaculture Research. 2020; 52 (4):1395-1400.
Chicago/Turabian StyleCuneyt Tamer; Yuksel Durmaz; Emre Ozan; Hamza Kadi; Abdullah Cavunt; Bahadir Muftuoglu; Harun Albayrak. 2020. "Pathogenicity trials regarding Turkish isolates of infectious pancreatic necrosis virus and viral haemorrhagic septicaemia virus in rainbow trout." Aquaculture Research 52, no. 4: 1395-1400.
Bovine respiratory disease (BRD) is a huge economic burden on the livestock industries of countries worldwide. Bovine respiratory syncytial virus (BRSV) is one of the most important pathogens that contributes to BRD. In this study, we report the identification and first isolation, with molecular characterization, of a new BRSV strain from lung specimens of three beef cows in Turkey that died from respiratory distress. After the screening of lung tissues for BRD-associated viruses using a multiscreen antigen-ELISA, a BRSV antigen was detected. This was then confirmed by real-time RT-PCR specific for BRSV. Following confirmation, virus isolation was conducted in MDBK cell cultures and clear CPE, including syncytia compatible with BRSV, were detected. RT-nested PCR, using F gene-specific primers, was performed on the cultured isolates, and the products were sequenced and deposited to Genbank with accession numbers MT179304, MT024766, and MT0244767. Phylogenetic analysis of these sequences indicated that the cattle were infected with BRSV from subgroup III and were closely related to previously identified American and Turkish strains, but contained some amino acid and nucleotide differences. This research paves the way for further studies on the molecular characteristics of natural BRSV isolates, including full genome analysis and disease pathogenesis, and also contributes to the development of robust national strategies against this virus.
Zafer Yazici; Emre Ozan; Cuneyt Tamer; Bahadir Muftuoglu; Gerald Barry; Hanne Nur Kurucay; Ahmed Eisa Elhag; Abdurrahman Anil Cagirgan; Semra Gumusova; Harun Albayrak. Circulation of Indigenous Bovine Respiratory Syncytial Virus Strains in Turkish Cattle: The First Isolation and Molecular Characterization. Animals 2020, 10, 1700 .
AMA StyleZafer Yazici, Emre Ozan, Cuneyt Tamer, Bahadir Muftuoglu, Gerald Barry, Hanne Nur Kurucay, Ahmed Eisa Elhag, Abdurrahman Anil Cagirgan, Semra Gumusova, Harun Albayrak. Circulation of Indigenous Bovine Respiratory Syncytial Virus Strains in Turkish Cattle: The First Isolation and Molecular Characterization. Animals. 2020; 10 (9):1700.
Chicago/Turabian StyleZafer Yazici; Emre Ozan; Cuneyt Tamer; Bahadir Muftuoglu; Gerald Barry; Hanne Nur Kurucay; Ahmed Eisa Elhag; Abdurrahman Anil Cagirgan; Semra Gumusova; Harun Albayrak. 2020. "Circulation of Indigenous Bovine Respiratory Syncytial Virus Strains in Turkish Cattle: The First Isolation and Molecular Characterization." Animals 10, no. 9: 1700.
A respiratory disease outbreak on a cattle farm in northern Turkey produced respiratory tract symptoms and severe pneumonia symptoms in 20 calves. Eight calves died, and a lung specimen from one carcass was analysed for bacteria and for viruses of the Bovine respiratory diseases complex. Bacteriological analysis was negative, but antigen detection ELISA and RT-PCR results indicated the presence of Bovine parainfluenza virus (BPIV). Virus isolation succeeded on Madin-Darby Bovine Kidney cells, and subsequent whole genome sequencing and phylogenetic analysis identified BPIV-3c. This is the first report of BPIV-3c isolation from cattle in Turkey, indicating the need for more virological and epidemiological studies.
Harun Albayrak; Zafer Yazici; Emre Ozan; Cuneyt Tamer; Ahmed Abd El Wahed; Stefanie Wehner; Kristina Ulrich; Manfred Weidmann. Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey. Veterinary Sciences 2019, 6, 56 .
AMA StyleHarun Albayrak, Zafer Yazici, Emre Ozan, Cuneyt Tamer, Ahmed Abd El Wahed, Stefanie Wehner, Kristina Ulrich, Manfred Weidmann. Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey. Veterinary Sciences. 2019; 6 (2):56.
Chicago/Turabian StyleHarun Albayrak; Zafer Yazici; Emre Ozan; Cuneyt Tamer; Ahmed Abd El Wahed; Stefanie Wehner; Kristina Ulrich; Manfred Weidmann. 2019. "Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey." Veterinary Sciences 6, no. 2: 56.