This study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticles and miRNA-133a probe onto the silicon dioxide of the fiber surface. For Mahlavu of hepatocellular carcinoma (HCC), detection is determined by the wavelength shift and transmission loss of a U-shaped optical fiber biosensor. The spectral sensitivity of wavelength and their coefficient of determination are found at −218.319 nm/ ng/mL and 0.839, respectively. Concurrently, the sensitivity of transmission loss and their coefficient of determination are found at 162.394 dB/ ng/mL and 0.984, respectively. A method for estimating the limit of detection of Mahlavu is at 0.0133 ng/mL. The results show that the proposed U-shaped biosensor is highly specific to miRNA-133a and possesses good sensitivity to variations in specimen concentration. As such, it could be of substantial value in microRNA detection.

Three functional materials were coated on tilted fiber Bragg grating (TFBG) sensors, and through the analysis of the characteristics of TBFG sensors’ amplitudes in the core and cladding modes, their abilities to sense humidity were compared. The mechanisms by which physical moisture absorption sensing was achieved through the three materials were investigated. The degree of sensitivity in terms of resonance wavelength in the cladding mode for the three coating materials was PAHP4 > PEDOT:PSS > GO. The sensitivity in the cladding modes of the tested sensor showed that the best resonance wavelength was as high as 0.0112 nm/% RH and was achieved for the TFBG coated with PAHP4 due to the hydrophilic groups around the material, which are good at forming hydrogen bond interactions with H₂O.

Rheumatoid arthritis (RA) is regarded as a chronic, immune-mediated disease that leads to the damage of various types of immune cells and signal networks, followed by inappropriate tissue repair and organ damage. RA is primarily manifested in the joints, but also manifests in the lungs and the vascular system. This study developed a method for the in vitro detection of RA through cyclic citrullinated peptide (CCP) antibodies and antigens. The diameter of a tilted-fiber Bragg grating (TFBG) biosensor was etched to 50 μm and then bonded with CCP antigens and antibodies. The small variations in the external refractive index and the optical fiber cladding were measured. The results indicated that the self-assembled layer of the TFBG biosensor was capable of detecting pre- and post-immune CCP antigen and CCP peptide concentrations within four minutes. A minimum CCP concentration of 1 ng/mL was detected with this method. This method is characterized by the sensor’s specificity, ability to detect CCP reactions, user-friendliness, and lack of requirement for professional analytical skills, as the detections are carried out by simply loading and releasing the test samples onto the platform. This study provides a novel approach to medical immunosensing analysis and detection. Although the results for the detection of different concentrations of CCP antigen are not yet clear, it was possible to prove the concept that the biosensor is feasible even if the measurement is not easy and accurate at this stage. Further study and improvement are required.

A temperature sensor was fabricated with a functional conductive poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coating on a long-period fiber grating (LPFG). The LPFG was fabricated by laser-assisted wet-chemical etching for controlling the grating depth of the LPFG after the treated surface of an optical fiber was inscribed by laser light. The functional conductive polymer acts as a temperature sustained sensing layer and enhances the grating depth of the LPFG sensor as a strain buffer at various temperatures. The sensor was subjected to three cycles of temperature measurement to investigate the sensor’s wavelength shift and energy loss when exposed to temperatures between 30 and 100 °C. Results showed that the sensor’s average wavelength sensitivity and its linearity were 0.052 nm/°C and 99%, respectively; average transmission sensitivity and linearity were 0.048 (dB/°C) and 95%, respectively.