We present a review on the natural infection by trypanosomatids of nonhuman vertebrates in Chile, aiming to synthesize and update the knowledge on the diversity of trypanosomatids infecting native and alien vertebrate species. To this end, we conducted a systematic review of literature records published from 1900 to April 2020 on four databases, focusing on the 21 genera of trypanosomatids and Chile. The methods and findings of our review have been based on the preferred reporting items for systematic reviews and meta-analysis (prisma) checklist. We found 29,756 records but only 71 presented relevant information for this review. Overall, there are only two reported trypanosomatid genera infecting vertebrate species in Chile, the genera Trypanosoma and Leishmania. The former is mostly represented by Trypanosoma cruzi (90% of the total records) and to a much lesser extent by Trypanosoma avium, Trypanosoma humboldti, Trypanosoma lewisi, and a couple of unidentified trypanosomatids. A total of 25 mammals have been reported as being infected by T. cruzi, including 14 native and 11 alien species from Orders Artiodactyla, Carnivora, Chiroptera, Didelphimorphia, Lagomorpha, Perissodactyla, and Rodentia. Extensive screening studies using new analytical tools are necessary to grasp the whole potential diversity of trypanosomatid species infecting vertebrates in Chile.

The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the globa...

Chagas disease, caused by Trypanosoma cruzi, is maintained in nature by complex interactions among wild vertebrates and triatomine insect vectors, yet the role of many introduced hosts remains poorly resolved. Here, we assessed natural T. cruzi infection in wild European rabbits (Oryctolagus cuniculus) from central Chile, where introduced rabbits overlap ecologically with the sylvatic vector Mepraia spinolai. Eight free-ranging rabbits captured in Las Chinchillas National Reserve were evaluated using an integrative diagnostic approach combining xenodiagnosis with laboratory-reared, parasite-free M. spinolai nymphs, real-time polymerase chain reaction targeting T. cruzi satellite DNA in blood and 12–14 organs per animal, and histopathology with immunohistochemistry (anti-cruzipain) to identify tissue parasite forms. Blood molecular detection was positive in seven out of eight rabbits, while xenodiagnosis detected viable parasites in two out of seven evaluated individuals. Organ molecular screening detected T. cruzi DNA in at least one organ in all rabbits, with frequent positivity in the diaphragm, reproductive tissues, spleen, and kidney. Histopathology identified parasite forms in four out of eight animals, and immunohistochemistry confirmed hepatic amastigotes in one case. These findings provide multi-method evidence of natural infection in the sampled individuals, including evidence of parasite viability in some individuals, suggesting potential epidemiological relevance within this ecological context and possible utility for surveillance in Chilean sylvatic transmission settings.

We developed an ethogram of Conepatus chinga (South American hog-nosed skunk, chingue) from the continuous monitoring of a captive individual, with environmental enrichment, at a wildlife rehabilitation center in the O’Higgins Region, Chile. Using video camera traps, 17 days (408 h) of its activity were recorded, allowing us to identify daily activity patterns and times of exploration, locomotion, feeding, shelter use, and other relevant behaviors. The individual displayed a constant nocturnal activity pattern, starting around 9:00 P.M. and finishing at 5:00 A.M. The most frequent behaviors were walking and sniffing, evidencing active exploration guided by smell. No hunting/scavenging attempts were observed under these husbandry conditions. The primary refuge used was a small tunnel (approximately 30 cm deep) excavated under a wooden pallet covered with tree branches, which is consistent with refuges reported in wildlife. No visible defecation, urination, or musk release was recorded in the camera-trap footage. Given the limited observation coverage, we cannot determine whether these behaviors did not occur or occurred outside the recorded field of view. This study provides behavioral information and a descriptive baseline for this individual, including a pilot ethogram useful for research and captive management.