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Dr. Abhishek Sharma
Amity Food and Agriculture Foundation, Amity University Uttar Pradesh, Noida

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0 Bioremediation
0 Plant Protection
0 Plant-Microbe Interactions
0 Biopesticides
0 Soil Microbiolgy

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Journal article
Published: 08 July 2021 in Journal of Environmental Chemical Engineering
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The degradation of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) through bioaugmentation and biostimulation was investigated. Aqueous phase experiment established the potential of Kinneretia asachharophila strain 12853 in invigorating the RDX-degradation. The microbe denitrified 75.7% RDX from the minimal salt medium by utilizing it as a nitrogen source based on nitrite production. High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LCMS) analyses confirmed the presence of methylenedinitramine (MEDINA) and 4-nitro-2,4-diazabutanal (NDAB) as intermediate metabolites. The microbe formulated into powder and beads showed 29% and 14% loss in viability, respectively, after a storage period of 3 months at 25 °C. The third type of formulation, i.e., water-dispersible granules (WDG), retained 98.52% viability at similar experimental conditions. The microcosm studies revealed a 40% RDX degradation in soil using WDG, while unformulated fresh cultures showed 29% degradation. The process became 1.9–2.4 times more efficient when WDG coupled with stimulants, wheat straw, or sucrose (0.5% w/w). We recorded first-order degradation kinetics with an RDX soil half-life of 16.54–24.15 days. The current study demonstrates the enhanced RDX remediation from the contaminated soil using a robust microbial formulation compatible with wheat straw as an organic stimulant.

ACS Style

Mohd Aamir Khan; Abhishek Sharma; Sonal Yadav; S. Mary Celin; Satyawati Sharma; Ahmed Noureldeen; Hadeer Darwish. Enhancing remediation of RDX-contaminated soil by introducing microbial formulation technology coupled with biostimulation. Journal of Environmental Chemical Engineering 2021, 9, 106019 .

AMA Style

Mohd Aamir Khan, Abhishek Sharma, Sonal Yadav, S. Mary Celin, Satyawati Sharma, Ahmed Noureldeen, Hadeer Darwish. Enhancing remediation of RDX-contaminated soil by introducing microbial formulation technology coupled with biostimulation. Journal of Environmental Chemical Engineering. 2021; 9 (5):106019.

Chicago/Turabian Style

Mohd Aamir Khan; Abhishek Sharma; Sonal Yadav; S. Mary Celin; Satyawati Sharma; Ahmed Noureldeen; Hadeer Darwish. 2021. "Enhancing remediation of RDX-contaminated soil by introducing microbial formulation technology coupled with biostimulation." Journal of Environmental Chemical Engineering 9, no. 5: 106019.

Original research article
Published: 10 June 2021 in Frontiers in Microbiology
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The current study investigates the diversity pattern and fungicidal potential of bacterial endophytes isolated from two different organic varieties of tomato plants (V1 and V2). A total of seventy-five bacterial isolates identified by 16S rRNA gene sequencing revealed a majority of genus as Bacillus and one Planococcus, which were grouped into eight different species. The Shannon diversity H’ (1.56), Simpson’s index of diversity (0.93), Magalef’ index (2.23), Evenness (0.96), and Species richness (7) indicated the high endophytic bacterial diversity in the V1 variety of the tomato. Bacterial endophytes isolated from both of the varieties were screened for their antifungal activity against five economically critical fungal pathogens (viz., Botrytis cinerea, Rhizoctonia solani, Fusarium solani, Verticillium lateritium, and Alternaria solani) of tomato crop through dual culture assay. The data revealed B. siamensis strain NKIT9 as the most potent antagonist, significantly (p < 0.05) inhibiting the mycelial growth between 75 to 90% against selected fungal pathogens. High bioactivity of lipopeptide extract of strain NKIT9 was recorded against R. solani with minimum IC50 value of 230 μg/ml. The Ultra Performance Liquid Chromatography-High Definition Mass Spectrometry (UPLC-HDMS) analysis of this lipopeptide extract revealed the presence of Surfactin and Bacillomycin D. Furthermore, in-vitro results showed that the selected bacterial strain significantly minimized the disease incidence in damping-off assay which makes this strain a promising antifungal bio-control agent. Moreover, in the pot experiment the NKIT9 increased the fruit yield by 59.2% compared with the untreated R. solani infested control.

ACS Style

Ayushi Sharma; Nutan Kaushik; Abhishek Sharma; Abhay Bajaj; Mandar Rasane; Yogesh S. Shouche; Takwa Marzouk; Naceur Djébali. Screening of Tomato Seed Bacterial Endophytes for Antifungal Activity Reveals Lipopeptide Producing Bacillus siamensis Strain NKIT9 as a Potential Bio-Control Agent. Frontiers in Microbiology 2021, 12, 1 .

AMA Style

Ayushi Sharma, Nutan Kaushik, Abhishek Sharma, Abhay Bajaj, Mandar Rasane, Yogesh S. Shouche, Takwa Marzouk, Naceur Djébali. Screening of Tomato Seed Bacterial Endophytes for Antifungal Activity Reveals Lipopeptide Producing Bacillus siamensis Strain NKIT9 as a Potential Bio-Control Agent. Frontiers in Microbiology. 2021; 12 ():1.

Chicago/Turabian Style

Ayushi Sharma; Nutan Kaushik; Abhishek Sharma; Abhay Bajaj; Mandar Rasane; Yogesh S. Shouche; Takwa Marzouk; Naceur Djébali. 2021. "Screening of Tomato Seed Bacterial Endophytes for Antifungal Activity Reveals Lipopeptide Producing Bacillus siamensis Strain NKIT9 as a Potential Bio-Control Agent." Frontiers in Microbiology 12, no. : 1.

Review
Published: 13 April 2021 in Microorganisms
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Capsicum annuum L. is a significant horticulture crop known for its pungent varieties and used as a spice. The pungent character in the plant, known as capsaicinoid, has been discovered to have various health benefits. However, its production has been affected due to various exogenous stresses, including diseases caused by a soil-borne pathogen, Pythium spp. predominantly affecting the Capsicum plant in younger stages and causing damping-off, this pathogen can incite root rot in later plant growth stages. Due to the involvement of multiple Pythium spp. and their capability to disperse through various routes, their detection and diagnosis have become crucial. However, the quest for a point-of-care technology is still far from over. The use of an integrated approach with cultural and biological techniques for the management of Pythium spp. can be the best and most sustainable alternative to the traditionally used and hazardous chemical approach. The lack of race-specific resistance genes against Pythium spp. can be compensated with the candidate quantitative trait loci (QTL) genes in C. annuum L. This review will focus on the epidemiological factors playing a major role in disease spread, the currently available diagnostics in species identification, and the management strategies with a special emphasis on Pythium spp. causing damping-off and root rot in different cultivars of C. annuum L.

ACS Style

Himanshu Arora; Abhishek Sharma; Satyawati Sharma; Farah Haron; Abdul Gafur; R. Sayyed; Rahul Datta. Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management. Microorganisms 2021, 9, 823 .

AMA Style

Himanshu Arora, Abhishek Sharma, Satyawati Sharma, Farah Haron, Abdul Gafur, R. Sayyed, Rahul Datta. Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management. Microorganisms. 2021; 9 (4):823.

Chicago/Turabian Style

Himanshu Arora; Abhishek Sharma; Satyawati Sharma; Farah Haron; Abdul Gafur; R. Sayyed; Rahul Datta. 2021. "Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management." Microorganisms 9, no. 4: 823.

Journal article
Published: 04 March 2021 in Journal of King Saud University - Science
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The present study explored the feasibility of waste biomass viz., Karanja (Pongamia pinnata) deoiled cake (KDC), and sundried biogas slurry (BGS) as a mixed substrate to develop Purpureocillium lilacinum 6029 based fungal formulation against plant-parasitic nematode, Meloidogyne incognita. The fungus cultured on KDC-BGS in a 60/40 ratio showed maximum egg mass inhibition (96.8%) and protease activity (376.65 U/g) along with superior colonization ability (100% colonization of egg mass on the third day). Incidentally, P. lilacinum spores obtained from the traditional substrate (wheat) inhibited only 68.72% egg mass hatching with a comparatively poor colonization rate construing the linkage between the nature of substrate and virulence of the fungus. Bioefficacy studies on tomato plants in the greenhouse revealed that the formulation with KDC-BGS as a substrate was 1.19 times more effective than the wheat-based formulation in controlling nematodes. Enhanced physical-biochemical parameters of tomato plants treated with the bioformulation further substantiated KDC-BGS as a reliable and economical substrate for P. lilacinum.

ACS Style

Abhishek Sharma; Satyawati Sharma; Naved Sabir; Mohamed A. El-Sheikh; Mohammed Alyemeni. Impact assessment of Karanja deoiled cake and sundried biogas slurry as a mixed substrate on the nematicidal potential of Purpureocillium lilacinum. Journal of King Saud University - Science 2021, 33, 101399 .

AMA Style

Abhishek Sharma, Satyawati Sharma, Naved Sabir, Mohamed A. El-Sheikh, Mohammed Alyemeni. Impact assessment of Karanja deoiled cake and sundried biogas slurry as a mixed substrate on the nematicidal potential of Purpureocillium lilacinum. Journal of King Saud University - Science. 2021; 33 (3):101399.

Chicago/Turabian Style

Abhishek Sharma; Satyawati Sharma; Naved Sabir; Mohamed A. El-Sheikh; Mohammed Alyemeni. 2021. "Impact assessment of Karanja deoiled cake and sundried biogas slurry as a mixed substrate on the nematicidal potential of Purpureocillium lilacinum." Journal of King Saud University - Science 33, no. 3: 101399.

Journal article
Published: 22 February 2021 in Journal of Pure and Applied Microbiology
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Brinjal and tomato are the most important transplanted vegetable crops of the Solanaceae family. The successful cultivation of these crops is vital for meeting the nutritional dietary requirement of India’s population and earning foreign exchange for the country by exporting vegetables to foreign countries. However, there are several abiotic and biotic impediments in the cultivation of these crops. Among biotic impediments , plant-parasitic nematodes have become one of the critical factor adversely affecting the cultivation of these vegetables. In general, Meloidogyne spp. (root-knot nematode) is the most common, widespread and economically damaging plant parasitic nematode species in tomato and brinjal crop. In addition to the damage caused by root – knot nematode, it stimulates the entry of soil-borne pathogens leading to development of the disease complex. The present study was undertaken to study the interaction effect of soil & soilless growing media viz. cocopeat and vermicompost along with organic amendments i.e., Trichoderma, AM fungus, and Cabbage residue incorporated individually as well as in different combinations for eco-friendly root-knot nematode management in brinjal and tomato nursery. The results indicated that treatment C-8 (Cocopeat + Trichoderma + AM fungus + Cabbage residues) recorded the superior germination count, germination percentage, days to 50% germination, root length, shoot length, fresh weight, root weight, shoot weight and root: shoot ratio. It is pertinent to mention that the soilless media, along with various organic amendments, were found to be superior for all the root and shoot attributes as compared to the conventional soil media for growing healthy nursery of tomato and brinjal in root knot nematode infested geographies. Our findings provide an effective and sustainable method of growing healthy plant nursery in nematode infested regions.

ACS Style

Ambuj Bhardwaj; Abhishek Sharma; B.K. Goswami; Vijay Bhardwaj. Interaction Effect of Soilless Media and Organic Amendments for Eco-Friendly Root-Knot Nematode Management in Brinjal and Tomato Nursery. Journal of Pure and Applied Microbiology 2021, 15, 356 -367.

AMA Style

Ambuj Bhardwaj, Abhishek Sharma, B.K. Goswami, Vijay Bhardwaj. Interaction Effect of Soilless Media and Organic Amendments for Eco-Friendly Root-Knot Nematode Management in Brinjal and Tomato Nursery. Journal of Pure and Applied Microbiology. 2021; 15 (1):356-367.

Chicago/Turabian Style

Ambuj Bhardwaj; Abhishek Sharma; B.K. Goswami; Vijay Bhardwaj. 2021. "Interaction Effect of Soilless Media and Organic Amendments for Eco-Friendly Root-Knot Nematode Management in Brinjal and Tomato Nursery." Journal of Pure and Applied Microbiology 15, no. 1: 356-367.

Book chapter
Published: 27 January 2021 in Nano- and Microencapsulation - Techniques and Applications
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Microemulsion formulation is among the most suitable carrier for the delivery of bioactive and, therefore, has excellent potential for industrial applications. The microemulsion system is thermodynamically and kinetically stable. Due to the smaller droplet size of the microemulsion system, the bioactive covers a larger surface of the target pest. Botanicals and essential oils, in particular, are green options to control various soil and seed-borne pathogens. Each oil contains several bioactive constituents that practically avoid microbe-resistance against it. Nevertheless, to improve the handling and shelf-life of botanicals, microemulsion formulation is the best option available. The current chapter provides the insight of a microemulsion system and explores the possibility of botanical oil-based biopesticides for a sustainable agro-ecosystem. We believe that botanical oil microemulsion could be a better alternative to synthetic pesticides and opens a new corridor for the promotion of the greener way of plant protection in India and across the globe.

ACS Style

Abhishek Sharma; Saurabh Dubey; Nusrat Iqbal. Microemulsion Formulation of Botanical Oils as an Efficient Tool to Provide Sustainable Agricultural Pest Management. Nano- and Microencapsulation - Techniques and Applications 2021, 1 .

AMA Style

Abhishek Sharma, Saurabh Dubey, Nusrat Iqbal. Microemulsion Formulation of Botanical Oils as an Efficient Tool to Provide Sustainable Agricultural Pest Management. Nano- and Microencapsulation - Techniques and Applications. 2021; ():1.

Chicago/Turabian Style

Abhishek Sharma; Saurabh Dubey; Nusrat Iqbal. 2021. "Microemulsion Formulation of Botanical Oils as an Efficient Tool to Provide Sustainable Agricultural Pest Management." Nano- and Microencapsulation - Techniques and Applications , no. : 1.

Journal article
Published: 21 December 2020 in Journal of Fungi
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Aspergillus species are known to cause damage to food crops and are associated with opportunistic infections in humans. In the United States, significant losses have been reported in peanut production due to contamination caused by the Aspergillus species. This study evaluated the antifungal effect and anti-aflatoxin activity of selected plant-based essential oils (EOs) against Aspergillus flavus in contaminated peanuts, Tifguard, runner type variety. All fifteen essential oils, tested by the poisoned food technique, inhibited the growth of A. flavus at concentrations ranging between 125 and 4000 ppm. The most effective oils with total clearance of the A. flavus on agar were clove (500 ppm), thyme (1000 ppm), lemongrass, and cinnamon (2000 ppm) EOs. The gas chromatography-mass spectrometry (GC-MS) analysis of clove EO revealed eugenol (83.25%) as a major bioactive constituent. An electron microscopy study revealed that clove EO at 500 ppm caused noticeable morphological and ultrastructural alterations of the somatic and reproductive structures. Using both the ammonia vapor (AV) and coconut milk agar (CMA) methods, we not only detected the presence of an aflatoxigenic form of A. flavus in our contaminated peanuts, but we also observed that aflatoxin production was inhibited by clove EO at concentrations between 500 and 2000 ppm. In addition, we established a correlation between the concentration of clove EO and AFB1 production by reverse-phase high-performance liquid chromatography (HPLC). We demonstrate in our study that clove oil could be a promising natural fungicide for an effective bio-control, non-toxic bio-preservative, and an eco-friendly alternative to synthetic additives against A. flavus in Georgia peanuts.

ACS Style

Premila Narayana Achar; Pham Quyen; Emmanuel C. Adukwu; Abhishek Sharma; Huggins Zephaniah Msimanga; Hanumanthu Nagaraja; Marikunte Yanjarappa Sreenivasa. Investigation of the Antifungal and Anti-Aflatoxigenic Potential of Plant-Based Essential Oils against Aspergillus flavus in Peanuts. Journal of Fungi 2020, 6, 383 .

AMA Style

Premila Narayana Achar, Pham Quyen, Emmanuel C. Adukwu, Abhishek Sharma, Huggins Zephaniah Msimanga, Hanumanthu Nagaraja, Marikunte Yanjarappa Sreenivasa. Investigation of the Antifungal and Anti-Aflatoxigenic Potential of Plant-Based Essential Oils against Aspergillus flavus in Peanuts. Journal of Fungi. 2020; 6 (4):383.

Chicago/Turabian Style

Premila Narayana Achar; Pham Quyen; Emmanuel C. Adukwu; Abhishek Sharma; Huggins Zephaniah Msimanga; Hanumanthu Nagaraja; Marikunte Yanjarappa Sreenivasa. 2020. "Investigation of the Antifungal and Anti-Aflatoxigenic Potential of Plant-Based Essential Oils against Aspergillus flavus in Peanuts." Journal of Fungi 6, no. 4: 383.

Preprint content
Published: 09 October 2020
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The current study investigates the diversity pattern and fungicidal potential of bacterial endophytes isolated from two different organic varieties of tomato plants (V1 and V2). A total of seventy-four bacterial isolates identified by 16S rRNA sequencing revealed a single genus Bacillus with 16 different species. The Shannon diversity H' (1.45), Simpson's index of diversity (0.9), Magalef' index (2.1), Evenness (0.96), and Species richness (8) indicated the high endophytic bacterial diversity in the V1 variety of the tomato. Bacterial endophytes isolated from both the varieties were screened for their antifungal activity against five economically critical fungal pathogens (viz., Botrytis cinerea, Rhizoctonia solani, Fusarium solani, Verticillium lateritium, and Alternaria solani) of tomato crop through dual culture assay. The data revealed B. siamensis KCTC 13613(T) as the most potent antagonist significantly (p < 0.05), inhibiting the mycelial growth between 75 to 90% against selected fungal pathogens. High bioactivity of lipopeptide extract of B. siamensis was recorded against R. solani with IC50 value of 72 ppm. The UPLC-HDMS analysis of this lipopeptide extract revealed the presence of, Surfactin and Bacillomycin D.

ACS Style

Ayushi Sharma; Nutan Kaushik; Abhishek Sharma; Abhay Bajaj; Mandar Rasane; Yogesh S Shouche; Takwa Marzouk; Naceur Djebali. Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent. 2020, 1 .

AMA Style

Ayushi Sharma, Nutan Kaushik, Abhishek Sharma, Abhay Bajaj, Mandar Rasane, Yogesh S Shouche, Takwa Marzouk, Naceur Djebali. Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent. . 2020; ():1.

Chicago/Turabian Style

Ayushi Sharma; Nutan Kaushik; Abhishek Sharma; Abhay Bajaj; Mandar Rasane; Yogesh S Shouche; Takwa Marzouk; Naceur Djebali. 2020. "Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent." , no. : 1.

Journal article
Published: 08 October 2020 in Sustainability
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Root-knot nematodes pose a serious threat to agriculture and forest systems, causing significant losses of the crop worldwide. A wide range of chemical nematicides has traditionally been used to manage phyto-nematodes. However, due to their ill effects on the environment, biological control agents (BCAs) like Purpureocillium lilacinumthat exhibit antagonistic effects on root-knot nematodes are preferred. The current study focused on identifying nematicidal metabolites produced by the fungus Purpureocillium lilacinum cultivated on akaranja deoiled cake-based liquid medium through bioactivity-guided fractionation against Meloidogyne incognita. Column chromatography of the ethyl acetate extract of fungal filtrate exhibited the most potent fraction (fraction 14–15), giving 94.6% egg mass hatching inhibition on the 5th day and a maximum nematicidal activity of 62% against second-stage juveniles after 48 h at 5000 mg/L. Gas chromatography coupled with mass spectrometry (GC-MS) analysis of this fraction revealed five major compounds, viz., 2-ethyl butyric acid, phenyl ethyl alcohol, benzoic acid, benzene acetic acid, and 3,5-Di-t-butylphenol. These biocompounds have potential biocontrol applications in agriculture, but further in vivo studies are warranted.

ACS Style

Abhishek Sharma; Aditi Gupta; Manu Dalela; Satyawati Sharma; R. Sayyed; Hesham Enshasy; Elsayed Elsayed. Linking Organic Metabolites as Produced by Purpureocillium lilacinum 6029 Cultured on Karanja Deoiled Cake Medium for the Sustainable Management of Root-Knot Nematodes. Sustainability 2020, 12, 8276 .

AMA Style

Abhishek Sharma, Aditi Gupta, Manu Dalela, Satyawati Sharma, R. Sayyed, Hesham Enshasy, Elsayed Elsayed. Linking Organic Metabolites as Produced by Purpureocillium lilacinum 6029 Cultured on Karanja Deoiled Cake Medium for the Sustainable Management of Root-Knot Nematodes. Sustainability. 2020; 12 (19):8276.

Chicago/Turabian Style

Abhishek Sharma; Aditi Gupta; Manu Dalela; Satyawati Sharma; R. Sayyed; Hesham Enshasy; Elsayed Elsayed. 2020. "Linking Organic Metabolites as Produced by Purpureocillium lilacinum 6029 Cultured on Karanja Deoiled Cake Medium for the Sustainable Management of Root-Knot Nematodes." Sustainability 12, no. 19: 8276.

Article
Published: 09 April 2020 in Phytochemistry Reviews
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Milletia pinnata, popularly known as Karanja, is a perennial tropical tree indigenous to India, South-Eastern Asia, and Australia. This highly favored oilseed plant is used in agroforestry as well as for restoration of wastelands due to its moderate tolerance to drought and salinity. Karanja tree, though widely investigated as biofuel feedstock producer, possess wider commercializing values that can be exploited in agricultural and industrial sectors. The oil, leaves, and bark of the Karanja tree have remarkable medicinal properties. In contrast, oil cakes have broad pesticidal applications due to the presence of various bioactive constituents (e.g., karanjin and pongamol). Due to richness in proteins and fatty acids, oil cakes have also been utilized as feedstock for industrially relevant enzyme production and animal feed. In this paper, we have presented the recent and updated review of varied applications of Karanja biomass classified into medicinal, industrial, and agricultural applications.

ACS Style

Abhishek Sharma; Nutan Kaushik; Himanshi Rathore. Karanja (Milletia pinnata (L.) Panigrahi): a tropical tree with varied applications. Phytochemistry Reviews 2020, 19, 643 -658.

AMA Style

Abhishek Sharma, Nutan Kaushik, Himanshi Rathore. Karanja (Milletia pinnata (L.) Panigrahi): a tropical tree with varied applications. Phytochemistry Reviews. 2020; 19 (3):643-658.

Chicago/Turabian Style

Abhishek Sharma; Nutan Kaushik; Himanshi Rathore. 2020. "Karanja (Milletia pinnata (L.) Panigrahi): a tropical tree with varied applications." Phytochemistry Reviews 19, no. 3: 643-658.

Journal article
Published: 28 March 2020 in Journal of Environmental Management
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In the current manuscript, we explored the remediation potential of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by Gram-positive Microbacterium esteraromaticum 12849. The strain detoxified 70.9 and 63.93% RDX in minimal nutrient medium and soil, respectively. Subsequently, the strain 12849 was formulated in form of water-dispersible granules (WDG) using talcum powder and alginic acid as inert ingredients. During the microcosm study, WDG exhibited 8.98% enhanced RDX degradation in contrast to the unformulated Microbacterium esteraromaticum. The LC-MS analysis revealed the presence of two intermediates, namely N-methyl-N, N′-dinitromethanediamine, and methylenedintramine, during the RDX degradation by strain 12849 in soil. Interestingly, no significant difference was observed in the rate of RDX degradation by strain 12849 due to the formulation process. The first-order kinetics was seen in RDX degradation with a degradation coefficient of 0.04 and 0.0339 day−1 by formulated and unformulated strain, respectively. The current investigation implies M. esteraromaticum as a potential microbe for RDX degradation and opens up the possibility of exploiting it in its effective WDG form for explosive contaminated sites.

ACS Style

Sonal Yadav; Abhishek Sharma; Mohd Aamir Khan; Ranju Sharma; Mary Celin; Anushree Malik; Satyawati Sharma. Enhancing hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX) remediation through water-dispersible Microbacterium esteraromaticum granules. Journal of Environmental Management 2020, 264, 110446 .

AMA Style

Sonal Yadav, Abhishek Sharma, Mohd Aamir Khan, Ranju Sharma, Mary Celin, Anushree Malik, Satyawati Sharma. Enhancing hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX) remediation through water-dispersible Microbacterium esteraromaticum granules. Journal of Environmental Management. 2020; 264 ():110446.

Chicago/Turabian Style

Sonal Yadav; Abhishek Sharma; Mohd Aamir Khan; Ranju Sharma; Mary Celin; Anushree Malik; Satyawati Sharma. 2020. "Enhancing hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX) remediation through water-dispersible Microbacterium esteraromaticum granules." Journal of Environmental Management 264, no. : 110446.

Journal article
Published: 01 February 2020 in Environmental Technology & Innovation
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ACS Style

Mohd Aamir Khan; Sonal Yadav; Ranju Sharma; Manu Dalela; S. Mary Celin; Abhishek Sharma; Satyawati Sharma. Augmentation of stimulated Pelomonas aquatica dispersible granules enhances remediation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) contaminated soil. Environmental Technology & Innovation 2020, 17, 1 .

AMA Style

Mohd Aamir Khan, Sonal Yadav, Ranju Sharma, Manu Dalela, S. Mary Celin, Abhishek Sharma, Satyawati Sharma. Augmentation of stimulated Pelomonas aquatica dispersible granules enhances remediation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) contaminated soil. Environmental Technology & Innovation. 2020; 17 ():1.

Chicago/Turabian Style

Mohd Aamir Khan; Sonal Yadav; Ranju Sharma; Manu Dalela; S. Mary Celin; Abhishek Sharma; Satyawati Sharma. 2020. "Augmentation of stimulated Pelomonas aquatica dispersible granules enhances remediation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) contaminated soil." Environmental Technology & Innovation 17, no. : 1.

Short communication
Published: 19 October 2018 in Indian Phytopathology
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In the present study, three strains each of Bacillus subtilis and Trichoderma harzianum were compared for their antagonism against Fusarium oxysporum f. sp. lycopersici (FOL), responsible for causing wilt disease in tomato plant. In vitro antifungal volatile assay revealed that B. subtilis 2274 caused significantly (p < 0.05) higher inhibition of radial growth of FOL (61.7%) compared to T. harzianum 3928 (47.2%). These two biocontrol agents with high efficacy (> 75%) were evaluated by profiling of volatile organic compounds (VOC). The emitted volatiles were extracted by non-invasive solid phase micro extraction (SPME) paired with gas chromatography-mass spectrometry (GC–MS). In total, 48 VOC were identified from B. subtilis 2274, and 20 from T. harzianum 3928. This study documented the VOC profile of efficient bacterial and fungal biocontrol strains mentioned above through a robust analytical method. It could successfully correlate the higher potential of B. subtilis as an antagonist as compared to T. harzianum with production of additional compounds of antifungal categories including derivatives of pyran, naphthalene and morpholine.

ACS Style

Monika Jangir; Ritika Pathak; Abhishek Sharma; Shilpi Sharma; Satyawati Sharma. Volatiles as strong markers for antifungal activity against Fusarium oxysporum f. sp. lycopersici. Indian Phytopathology 2018, 72, 681 -687.

AMA Style

Monika Jangir, Ritika Pathak, Abhishek Sharma, Shilpi Sharma, Satyawati Sharma. Volatiles as strong markers for antifungal activity against Fusarium oxysporum f. sp. lycopersici. Indian Phytopathology. 2018; 72 (4):681-687.

Chicago/Turabian Style

Monika Jangir; Ritika Pathak; Abhishek Sharma; Shilpi Sharma; Satyawati Sharma. 2018. "Volatiles as strong markers for antifungal activity against Fusarium oxysporum f. sp. lycopersici." Indian Phytopathology 72, no. 4: 681-687.

Journal article
Published: 01 October 2018 in Journal of Bioscience and Bioengineering
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Calocybe indica was cultivated on wheat straw enriched with various concentrations of sodium selenite (Na2SeO3), (2.5–40 μg/ml). The content of selenium (Se) in fruit bodies increased linearly by increasing the amount of Na2SeO3, although the biomass yield inhibited above 5 μg/ml. The fruit bodies accumulated inorganic Se into organic forms by integrating the Se into proteins (56%–68%), polysaccharides (22%–29%), and nucleic acids (1.4%–2.7%). Maximum protein content (25.31 g/100 g) was observed in fruit bodies harvested from the substrate enriched with 10 μg/ml Se. Amino acid profiling revealed the highest value of glutamic acid (4.73 g/100 g), followed by aspartic acid (1.80 g/100 g), and glycine (1.61 g/100 g) at 10 μg/ml. The Se enrichment also enhanced the total phenol, free radical 2,2-dipheynl-1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing power (FRAP) of methanolic extracts of fruiting bodies to almost double of their contents. The principle component analysis (PCA) illustrated close correlations amongst the biomass yield, polyphenols, and antioxidant activities at 5 μg/ml concentrations of the Se.

ACS Style

Himanshi Rathore; Abhishek Sharma; Shalinee Prasad; Satyawati Sharma. Selenium bioaccumulation and associated nutraceutical properties in Calocybe indica mushroom cultivated on Se-enriched wheat straw. Journal of Bioscience and Bioengineering 2018, 126, 482 -487.

AMA Style

Himanshi Rathore, Abhishek Sharma, Shalinee Prasad, Satyawati Sharma. Selenium bioaccumulation and associated nutraceutical properties in Calocybe indica mushroom cultivated on Se-enriched wheat straw. Journal of Bioscience and Bioengineering. 2018; 126 (4):482-487.

Chicago/Turabian Style

Himanshi Rathore; Abhishek Sharma; Shalinee Prasad; Satyawati Sharma. 2018. "Selenium bioaccumulation and associated nutraceutical properties in Calocybe indica mushroom cultivated on Se-enriched wheat straw." Journal of Bioscience and Bioengineering 126, no. 4: 482-487.

Original paper
Published: 09 August 2018 in Waste and Biomass Valorization
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In this study, we cultivated Calocybe indica (C. indica) on wheat straw basal substrate and analysed the influence of supplementation of nitrogenous tree leaves of Syzygium cumini (SCL), Bauhinia variegate (BVL) and Cassia fistula (CFL) on yield, nutritional composition, and antioxidant properties. Wheat straw basal substrate was supplemented with different proportions (25, 50, and 75%) of SCL, BVL and CFL to determine the most effective substrate composition. Results indicated that 25 BVL was the best substrate for producing highest yield (622 g) and biological efficiency (82.93%) of C. indica. Principle component analysis (PCA) was performed to observe the correlation within nutritional parameters (crude fat, crude protein, ash, fibre, carbohydrates, moisture and dry matter) and how these different parameters varied in different treatments used in this study. PC1 and PC2 represented 76.27% of total variation in the observed variables. Supplementation of leafy biomass significantly (p < 0.05) improved the minerals (Na, Mg, K, Ca, Mn, Fe, Zn, Se) and nutritional contents of the C. indica over wheat straw alone. PCA of mineral content (mg/100 g) of the C. indica grown on WS supplemented with nitrogenous tree leaves showed their grouping to PC1 and most of the mineral are positively correlated as depicted in correlation matrix. The impact of different substrates on the antioxidant activities of methanolic extracts of C. indica was also studied. Among the ten treatments, 25 BVL produced the fruiting bodies with high antioxidant properties followed by 25 CFL and 25 SCL. In conclusion, nitrogenous leafy biomass could be an economical, novel and easily accessible nitrogenous supplementation for C. indica cultivation.

ACS Style

Himanshi Rathore; Abhishek Sharma; Shalinee Prasad; Ashwini Kumar; Satyawati Sharma; Ajay Singh. Yield, Nutritional Composition and Antioxidant Properties of Calocybe indica Cultivated on Wheat Straw Basal Substrate Supplemented with Nitrogenous Tree Leaves. Waste and Biomass Valorization 2018, 11, 807 -815.

AMA Style

Himanshi Rathore, Abhishek Sharma, Shalinee Prasad, Ashwini Kumar, Satyawati Sharma, Ajay Singh. Yield, Nutritional Composition and Antioxidant Properties of Calocybe indica Cultivated on Wheat Straw Basal Substrate Supplemented with Nitrogenous Tree Leaves. Waste and Biomass Valorization. 2018; 11 (3):807-815.

Chicago/Turabian Style

Himanshi Rathore; Abhishek Sharma; Shalinee Prasad; Ashwini Kumar; Satyawati Sharma; Ajay Singh. 2018. "Yield, Nutritional Composition and Antioxidant Properties of Calocybe indica Cultivated on Wheat Straw Basal Substrate Supplemented with Nitrogenous Tree Leaves." Waste and Biomass Valorization 11, no. 3: 807-815.

Journal article
Published: 09 July 2018 in Industrial Crops and Products
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Essential oils play pivotal role in sustainably controlling plant pests and diseases. In this study, a synergistic oil-in-water nanoemulsion containing clove (CO) and lemongrass oil (LGO) was developed and explored its potential as antifungal agents against Fusarium oxysporum f.sp. lycopersici (FOL). After initial confirmation of synergism between CO-LGO, a nanoemulsion was formulated using non-ionic surfactants through low-energy emulsification method. The nanoemulsion showed Z-average diameter of 76.73 nm having spherical shaped droplet structure as confirmed by TEM imaging. Minimum inhibitory concentration (MIC) of the optimized nanoemulsion was 4000 mg/l against FOL with the logistic kinetic model demonstrating its notably rapid fungicidal effect. The nanoemulsion disrupted the membrane integrity of FOL as consistently evidenced by sodium dodecyl sulfate polyacrylamide gel electrophoresis, scanning electron and atomic force microscopy. During in planta assay, nanoformulation applied as a soil amendment at MIC reduced the severity of tomato Fusarium wilt incidence up to 70.6% compared with untreated control. The nanoemulsion applied on seedlings and seeds also significantly controlled the wilt disease in tomato without showing any sign of phytotoxicity.

ACS Style

Abhishek Sharma; Naveen Kumar Sharma; Ankit Srivastava; Arti Kataria; Saurabh Dubey; Satyawati Sharma; Bishwajit Kundu. Clove and lemongrass oil based non-ionic nanoemulsion for suppressing the growth of plant pathogenic Fusarium oxysporum f.sp. lycopersici. Industrial Crops and Products 2018, 123, 353 -362.

AMA Style

Abhishek Sharma, Naveen Kumar Sharma, Ankit Srivastava, Arti Kataria, Saurabh Dubey, Satyawati Sharma, Bishwajit Kundu. Clove and lemongrass oil based non-ionic nanoemulsion for suppressing the growth of plant pathogenic Fusarium oxysporum f.sp. lycopersici. Industrial Crops and Products. 2018; 123 ():353-362.

Chicago/Turabian Style

Abhishek Sharma; Naveen Kumar Sharma; Ankit Srivastava; Arti Kataria; Saurabh Dubey; Satyawati Sharma; Bishwajit Kundu. 2018. "Clove and lemongrass oil based non-ionic nanoemulsion for suppressing the growth of plant pathogenic Fusarium oxysporum f.sp. lycopersici." Industrial Crops and Products 123, no. : 353-362.

Original articles
Published: 06 March 2018 in Biofuels
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The current investigation deals with the application of microwave energy to facilitate alkali (NaOH) pretreatment on banana peel waste (BPW) to achieve optimal reducing sugar. We studied the effect of microwave power (100 to 600 W) and irradiating time (1 to 6 min) on composition and reducing sugar content of BPW. The enzymatic hydrolysis of microwave-assisted alkali-pretreated BPW at 600 W for 2 min gave the maximum reducing sugar (0.561 g/g) of dry biomass waste. Atomic force microscopy revealed that the surface of all BPW was changed noticeably when microwave-assisted alkali pretreatment was employed. X-ray diffraction study revealed a 5-fold increment in the degree of crystallinity in optimal microwave alkali-treated samples (54.45%) as compared to the untreated sample (10.55%). Overall, the microwave-assisted alkali pretreatment removed a significant amount of hemicelluloses and lignin and led to a high amount of sugar production, suggesting microwave heating pretreatment as a potential alternative to conventional methods to achieve maximal reducing sugars.

ACS Style

Garima Tiwari; Abhishek Sharma; Ashwani Kumar; Satyawati Sharma. Assessment of microwave-assisted alkali pretreatment for the production of sugars from banana fruit peel waste. Biofuels 2018, 10, 3 -10.

AMA Style

Garima Tiwari, Abhishek Sharma, Ashwani Kumar, Satyawati Sharma. Assessment of microwave-assisted alkali pretreatment for the production of sugars from banana fruit peel waste. Biofuels. 2018; 10 (1):3-10.

Chicago/Turabian Style

Garima Tiwari; Abhishek Sharma; Ashwani Kumar; Satyawati Sharma. 2018. "Assessment of microwave-assisted alkali pretreatment for the production of sugars from banana fruit peel waste." Biofuels 10, no. 1: 3-10.

Journal article
Published: 01 March 2017 in Journal of Bioscience and Bioengineering
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The antifungal effects of four essential oils viz., clove (Syzygium aromaticum), lemongrass (Cymbopogon citratus), mint (Mentha × piperita) and eucalyptus (Eucalyptus globulus) were evaluated against wilt causing fungus, Fusarium oxysporum f. sp. lycopersici 1322. The inhibitory effect of oils showed dose-dependent activity on the tested fungus. Most active being the clove oil, exhibiting complete inhibition of mycelial growth and spore germination at 125 ppm with IC50 value of 18.2 and 0.3 ppm, respectively. Essential oils of lemongrass, mint and eucalyptus were inhibitory at relatively higher concentrations. The Minimum inhibitory concentration (MIC) of clove oil was 31.25 ppm by broth microdilution method. Thirty one different compounds of clove oil, constituting approximately ≥99% of the oil, were identified by gas chromatography–mass spectroscopy analysis. The major components were eugenol (75.41%), E-caryophyllene (15.11%), α-humulene (3.78%) and caryophyllene oxide (1.13%). Effect of clove oil on surface morphology of F. oxysporum f. sp. lycopersici 1322 was studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). SEM observation revealed shrivelled hyphae while AFM observation showed shrunken and disrupted spores in clove oil treated samples. In pots, 5% aqueous emulsion of clove oil controlled F. oxysporum f. sp. lycopersici 1322 infection on tomato plants. This study demonstrated clove oil as potent antifungal agent that could be used as biofungicide for the control of F. oxysporum f. sp. lycopersici in both preventive and therapeutic manner.

ACS Style

Abhishek Sharma; Sasireka Rajendran; Ankit Srivastava; Satyawati Sharma; Bishwajit Kundu. Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil. Journal of Bioscience and Bioengineering 2017, 123, 308 -313.

AMA Style

Abhishek Sharma, Sasireka Rajendran, Ankit Srivastava, Satyawati Sharma, Bishwajit Kundu. Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil. Journal of Bioscience and Bioengineering. 2017; 123 (3):308-313.

Chicago/Turabian Style

Abhishek Sharma; Sasireka Rajendran; Ankit Srivastava; Satyawati Sharma; Bishwajit Kundu. 2017. "Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil." Journal of Bioscience and Bioengineering 123, no. 3: 308-313.

Journal article
Published: 31 December 2016 in Journal of Pure and Applied Microbiology
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ACS Style

Ritika Pathak; Abhishek Sharma; Anurup Adak; Satyawati Sharma; Rajendra Prasad. Role of Jatropha curcas Deoiled Cake as Substrate for the Production of Cellulases and Xylanase and Additive in Vermicomposting of Kitchen Waste. Journal of Pure and Applied Microbiology 2016, 10, 3163 -3172.

AMA Style

Ritika Pathak, Abhishek Sharma, Anurup Adak, Satyawati Sharma, Rajendra Prasad. Role of Jatropha curcas Deoiled Cake as Substrate for the Production of Cellulases and Xylanase and Additive in Vermicomposting of Kitchen Waste. Journal of Pure and Applied Microbiology. 2016; 10 (4):3163-3172.

Chicago/Turabian Style

Ritika Pathak; Abhishek Sharma; Anurup Adak; Satyawati Sharma; Rajendra Prasad. 2016. "Role of Jatropha curcas Deoiled Cake as Substrate for the Production of Cellulases and Xylanase and Additive in Vermicomposting of Kitchen Waste." Journal of Pure and Applied Microbiology 10, no. 4: 3163-3172.

Journal article
Published: 02 April 2016 in World Journal of Microbiology and Biotechnology
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In present study, in vitro nematocidal bioassays, FT-IR and HPLC analysis were employed to demonstrate the involvement of toxins of Purpureocillium lilacinum in killing root-knot nematodes (Meloidogyne incognita). During growth study, maximum mycelial biomass (10.52 g/l) in de-oiled Karanja cake medium was achieved on 8th day while complete mortality of nematodes was obtained by 6th day filtrate (FKSM). Maximum production of crude nematocidal toxin was recorded on 7th day suggesting that the toxin production was paralleled with growth of the fungus. The median lethal concentration (LC50) determined for the crude toxin from 6th day to 10th day ranged from 89.41 to 43.21 ppm. The median lethal time (LT50) for the crude toxin of FKSM was found to be 1.46 h. This is the first report of implementing a comparative infra-red spectroscopy coupled with HPLC analysis to predict the presence of nematocidal toxin in the fungal filtrate cultured on Karanja deoiled cake liquid medium.

ACS Style

Abhishek Sharma; Satyawati Sharma; Aditya Mittal; Satya Narayan Naik. Evidence for the involvement of nematocidal toxins of Purpureocillium lilacinum 6029 cultured on Karanja deoiled cake liquid medium. World Journal of Microbiology and Biotechnology 2016, 32, 1 -5.

AMA Style

Abhishek Sharma, Satyawati Sharma, Aditya Mittal, Satya Narayan Naik. Evidence for the involvement of nematocidal toxins of Purpureocillium lilacinum 6029 cultured on Karanja deoiled cake liquid medium. World Journal of Microbiology and Biotechnology. 2016; 32 (5):1-5.

Chicago/Turabian Style

Abhishek Sharma; Satyawati Sharma; Aditya Mittal; Satya Narayan Naik. 2016. "Evidence for the involvement of nematocidal toxins of Purpureocillium lilacinum 6029 cultured on Karanja deoiled cake liquid medium." World Journal of Microbiology and Biotechnology 32, no. 5: 1-5.