Background and Aim: Trichinellosis remains a dangerous disease for humans and animals, which can lead to a lethal outcome. The study of specific body reactions in response to invasion by different types of Trichinella can help in the early diagnosis of the disease. This study aimed to investigate the hematological, biochemical, and serological characteristics of rabbits experimentally infected with trichinellosis, as well as the possibility of using changes in these parameters at various disease stages for early hematological, biochemical, and serological diagnosis of trichinellosis. Materials and Methods: Three groups of rabbits were orally infected with Trichinella nativa and Trichinella spiralis derived from encysted T. spirtalis larvae in pork muscle samples. The first and second groups were infected with T. nativa and T. spiralis, respectively, while the third group served as control by receiving a physiological solution. An ADVIA 2120i automatic hematology analyzer with a blood smear staining module was used to determine the hematological parameters of rabbits. Antigens were used in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies in the sera of infected rabbits that were supernatants containing excretory-secretory antigens (ES-Ag) and somatic antigen (S-Ag). Results: The detection of biochemical responses to the invasion of T. nativa and T. spiralis isolates was detected and hematological parameters were featured in two cases. Trichinella nativa increased the number of erythrocytes, neutrophils, eosinophils, monocytes, basophils, and thrombocytes on day 7 in rabbits. Creatine kinase (CK) is regarded as the most important indicator for the early detection of parasite invasion. Blood biochemistry showed no active response to T. spiralis infection. However, counts of erythrocytes, neutrophils, lymphocytes, and CK rose significantly. In both color indicators, the number of thrombocytes decreased. Enzyme-linked immunosorbent assay with ES-Ag and S-Ag of these isolates demonstrated the ability to detect antibodies as early as 7 days after infection, with a significant increase in the marker up to 70 days. Conclusion: On the 7th day after infection, blood tests of infected animals revealed CK-N-acetyl-cysteine (18.2%) and neutrophils (43%) when infected with T. nativa and neutrophils (26.7%) and lymphocytes (20%) when infected with T. spiralis. These indicators may serve as specific parameters for the early detection of Trichinella spp. invasion.
This research is aimed at studying the properties of polymer anticorrosion coatings based on ED-20 resin widely used in practice and industrial wastes. In this work, three basic types of nanoscale nanofillers were chosen: dispersed particles—microsilica, microspheres obtained at Kazakh enterprises, and carbon nanotubes. Physicochemical research methods were used in the research: a laser analyzer for studying the dispersibility of industrial waste and spectrometric research methods. The properties of materials were investigated by standardized methods. The obtained results show that the introduction of microsilica and microspheres obtained at Kazakhstani enterprises, used as additives, improves both the physical and mechanical properties of epoxy composites compared to the standard (control) material. The results of experiments have shown that the optimal content of additives of microsilica and microspheres provides an improvement in the physical and mechanical properties of epoxy composites in comparison with the standard (control) material. Studies have shown that the introduction of microspheres into ED-20 polymer increases impact toughness. The introduction of microsilica into the matrix contributes to the increase of elastic modulus. Experimental studies of optical properties of samples of carbon composite polymer films based on polystyrene (PS) with additives of carbon nanotubes C60 and C70 and multilayer carbon nanotubes were also carried out. The experimental results obtained for the optical properties of polymer composites based on basic polymers from solid waste and carbon nanotubes showed that the optical properties of polymer composites undergo noticeable changes.
The global packaging market is undergoing rapid transformation. The amount of package consumption is increasing, the market structure is shifting, and new packaging technologies are emerging. Biodegradable packaging is a solution to environmental challenges that is gaining increasing attention from the international community. The development of packaging that does not affect the environment in the process of disposal is presently a top priority. The process for making biodegradable packaging from natural biomass, namely agricultural waste and solid home waste, wollastonite, is presented in this article. α-cellulose, lignin, ash, and hemicellulose were examined for chemical composition in packaging paper made from agricultural waste, specifically wheat straw and rice straw, using established procedures. In addition, studies were conducted on the transmission of micro- and nanoparticles through the prepared paper, a study was conducted on an FTIR instrument to obtain cellulose spectra, and a thermogravimetric investigation to determine whether the samples were degradable, as well as a non-standard method for the biodegradable properties of paper
This research explores the potential of agricultural waste, specifically flax straw, as a sustainable raw material for eco-friendly packaging materials. This study investigates a three-stage delignification process involving nitric acid, alkaline treatment, and organosolvent solutions. This method effectively removes lignin from the straw, resulting in high-quality technical pulp with 67.7% α-cellulose and a significantly reduced ash content (8.5%). X-ray diffraction (XRD) and thermogravimetric analysis (TGA) were employed to characterize the treated flax straw. XRD analysis revealed changes in the cellulose structure, while TGA indicated enhanced thermal stability compared to untreated straw. Microscopic analysis of the pulp fibers shows a parallel and aligned arrangement, suggesting a high fiber content and a strong paper lattice. The particle size distribution of the ground pulp, influenced by fiber size, has implications for the packing density and mechanical properties of the final product. This study demonstrates the potential of agricultural waste as a sustainable source for packaging materials, contributing to the circular economy and waste reduction.
9 May 2023 in Potravinarstvo Slovak Journal of Food Sciences
The article presents the organoleptic and physicochemical (humidity and strength) quality indicators of pasta with the addition of millet at 7.7, and 15.5%, as a new recipe for pasta production. Millets can be used to supplement pasta because of their superior nutritional value and health advantages. On the territory of the Republic of Kazakhstan and the Eurasian Economic Union, the quality indicators were calculated while taking into account the practices outlined in the standardized documents. Express drying, accelerated drying, drying to a constant mass, and employing the MA-30 "SARTORIUS" apparatus following interstate standards were all employed. The study aimed to achieve appropriate organoleptic quality indicators and physicochemical indicators of humidity up to 28% (after processing pasta with the addition of millet 7.7, and 15.5%. Approximately 100 trials were carried out at the Federal State Autonomous Scientific Institution "Scientific Research Institute of the Bakery Industry" Russian Federation, Moscow. According to the study's findings, all quality indicators are within acceptable ranges, except for pasta with the addition of millet 23.3%, recipes for pasta with the addition of millet have been developed, a utility model patent has been obtained in the territory of the Republic of Kazakhstan No. 7071, issued by the Republican State Enterprise on the right of economic management "National Institute of Intellectual Property". In conclusion, pasta recipes with the addition of millet have been developed. According to the study's findings, all quality indicators are within acceptable limits except pasta with the addition of millet, which accounts for 23.3% of the total.
31 August 2023 in Eastern-European Journal of Enterprise Technologies
Utilization of industrial waste and secondary raw materials, in particular, in the production of metallic silicon and silicon-containing alloys, which include silica vapors (microsilica), is the main task of implementing environmental policy and solves the problem of their storage and negative impact on the environment, in order to reuse them in polymer composites. The use of microsilica as a filler in composite materials based on epoxy resins contributes to a positive effect on the basic properties of the resin, and also makes it possible to use the composite material as coatings and parts in the repair of machinery and equipment. The aim of the work was to establish the positive effect of microsilica as a filler in composite materials based on ED-20 epoxy resin on improving the physical and mechanical properties of composite materials. Within the framework of this work, studies were conducted to test composite materials based on ED-20 epoxy resin, differing in different filler content of 2, 5, 10, 15 wt. % on impact strength, tensile strength and modulus of elasticity, adhesion and impact strength of the coating. The analysis of the obtained results showed a positive effect of microsilica as a filler in composite materials based on ED-20 epoxy resin on the physical and mechanical properties of the composite material. The optimal filler content was determined, which is 2 % of the mass of the ED-20 epoxy resin, while an increase in adhesion, toughness of the composite material by 45 %, tensile strength and modulus of elasticity by 21 % and 5 %, respectively, and the strength of the coating on impact by 32 %, compared with the addition of microsilica in ED-20, which shows the prospects of using microsilica as a filler in composite materials