In October 2020, samples of walnut branch blight were collected from Longnan. Pathogens were isolated and identified based on morphological and molecular features, and their characteristics were analyzed by pathogenicity. Pathogenicity testing revealed that seven strains (LN-1, LN-3, LN-6, LN-19, LN-27, QY3-1, and QY9-1) induced symptoms of walnut branch blight that were consistent with those observed in the field after inoculation. Furthermore, some Fusarium-type conidia and spherical chlamydospores were visible indicating that they were Fusarium spp. A molecular characterization including sequence and phylogenetic analysis of the ITS, TEF-1α, βTUB, Fu, and LSU gene regions revealed that LN-1 and LN-19 belonged to F. avenaceum, LN-3 and LN-6 to F. acuminatum, LN-27 to F. sporotrichioides, and QY3-1 and QY9-1 to F. tricinctum. This is the first time that F. acuminatum-, F. sporotrichioides-, and F. tricinctum-caused walnut branch blight has been reported in China.

Snow pea root rot in China is caused by Fusarium solani (FSH) and Fusarium avenaceum (FAH), which affect snow pea production. The chemical control methods used against FSH and FAH are toxic to the environment and resistance may be developed in persistence applications. Therefore, an alternative approach is needed to control these pathogens. This study focuses on Trichoderma longibrachiatum strains (TL6 and TL13), mycoparasitic mechanisms of FSH and FAH, as well as growth-promoting potentials on snow pea seedlings under FSH and FAH stress at the physiological, biochemical, and molecular levels. The average inhibitory rates of TL6 against FSH and FAH were 54.58% and 69.16%, respectively, on day 7. Similarly, TL13 average inhibitory rates against FSH and FAH were 59.06% and 71.27%, respectively, on day 7. The combined TL13 and TL6 with FSH and FAH reduced disease severity by 86.6, 81.6, 57.60, and 60.90%, respectively, in comparison to the controls. The snow pea plants inoculated with FSH and FAH without TL6 and TL13 increased malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) contents in the leaves by 64.8, 66.0, 64.4 and 65.9%, respectively, compared to the control. However, the combined FSH and FAH with TL6 and TL13 decreased the MDA and H₂O₂ content by 75.6, 76.8, 70.0, and 76.4%, respectively, in comparison to the controls. In addition, the combined TL6 + FSH and TL6 + FAH increased the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) by 60.5, 64.7, and 60.3%, respectively, and 60.0, 64.9, and 56.6%, respectively, compared to the controls. Again, compared to the controls, the combined TL13 + FSH and TL13 + FAH increased the activity of SOD, POD, and CAT by 69.7, 68.6, and 65.6%, respectively, and 70.10, 69.5, and 65.8%, respectively. Our results suggest that the pretreatment of snow pea seeds with TL6 and TL13 increases snow pea seedling growth, controls FSH and FAH root rot, increases antioxidant enzyme activity, and activates plant defense mechanisms. The TL13 strain had the greatest performance in terms of pathogen inhibition and snow pea growth promotion compared to the TL6 strain.

Globally, grasslands are affected by climate change and unsustainable management practices which usually leads to transitions from stable, degraded and then to desertification. Spatial vegetation patch configurations are regarded as key indicators of such transitions. Understanding the relationships between this grass-land vegetation and its environment is key to vegetation restoration projects. Spatial vegetation patch patterns were chosen across different soil and topographic conditions. Patch numbers, perimeter, and cover of each patch were measured along transects of each patch type. Using field surveys and multivariate statistical analysis, we investigated the differences in vegetation biomass and distribution and soil properties of four typical alpine plant species patches along with a range of environmental and topographic conditions. It was found that topographic conditions and soil properties, particularly soil moisture explained most of the variation in spatial patch vegetation characteristics and thus control vegetation restoration in the alpine grassland. The Kobresia humilis, Blysmus sinocompressus and Iris lactea patches under the drylands recorded small patch sizes, large patch numbers, low connectivity, and large total perimeter per unit area. Generally, species within the high moisture sites recorded small patch numbers, a large fraction of vegetation cover and a small total perimeter per m². Patches in limited soil moisture areas recorded patch configurations indicating they are unstable and undergoing degradation and therefore need urgent restoration attention to forestall their further degradation and its resultant effect of desertification. These results would provide quantitative easy-to-use indicators for vegetation degradation and help in vegetation restoration projects.

The study investigates the effects of NPK fertilizer and spent mushroom substrate (SMS) on lettuce (Lactuca sativa) growth, yield, and antioxidant enzyme activity. SMS, a by-product of mushroom cultivation, is a sustainable organic fertilizer rich in organic matter and beneficial microbes, while NPK provides essential macronutrients for plant growth. A greenhouse experiment was conducted using four treatments: control (no amendment), SMS, NPK, and a combination of SMS and NPK. Key growth parameters, including plant height, number of leaves, and fresh and dry weights of leaves and roots, were measured, alongside oxidative stress markers and antioxidant enzyme activities. The results revealed that SMS and NPK independently improved lettuce growth and physiological responses, but their combined application had the most significant effects. The SMS+NPK treatment increased plant height, leaf and root biomass, and antioxidant enzyme activities while reducing oxidative stress markers like MDA and H₂O₂. This synergistic effect underlines the potential of integrating organic and inorganic fertilizers to enhance crop productivity sustainably. The findings highlight the benefits of SMS as an environmentally friendly amendment, cost-effectiveness, and its potential to complement NPK for sustainable agricultural practices. SMS enhances soil microbial diversity and organic matter content more effectively than inorganic fertilizers, leading to improved nutrient cycling and long-term soil fertility. This research offers a novel application of SMS as a low-cost substitute for fertilizers that pose environmental risks.