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Prof. Dr. Aniello Anastasio
Department of Veterinary Medicine and Animal Production, University of Naples, Federico II, via Delpino 1, 80137, Naples, Italy

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0 Food Microbiology
0 One Health
0 Food Science and Technology
0 food inspection
0 Fish inspection

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Journal article
Published: 14 June 2021 in Foods
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Antimicrobial resistance has become one of the major global public health concerns, and it is indispensable to search for alternatives to conventional antibiotics. Recently, antimicrobial peptides have received great attention because of their broad-spectrum antimicrobial activity at relatively low concentrations, even against pathogens such as Salmonella enterica, which is responsible for most food-borne illnesses. This work aimed at evaluating the antimicrobial and antibiofilm activity of the innate defense peptide, named 1018-K6, against S. enterica. A total of 42 strains, belonging to three different subspecies and 32 serotypes, were included in this study. The antibiotic resistance profile of all the strains and the cytotoxic effects of 1018-K6 on mammalian fibroblast cells were also investigated. Results revealed that MIC (minimum inhibitory concentrations) and MBC (minimum bactericidal concentrations) values were in the ranges of 8–64 μg/mL and 16–128 μg/mL, respectively, although most strains (97%) showed MICs between 16 and 32 μg/mL. Moreover, sub-inhibitory concentrations of 1018-K6 strongly reduced the biofilm formation in several S. enterica strains, whatever the initial inoculum size. Our results demonstrated that 1018-K6 is able to control and manage S. enterica growth with a large potential for applications in the fields of active packaging and water disinfectants.

ACS Style

Rossella Festa; Rosa Ambrosio; Alexandre Lamas; Lorena Gratino; Gianna Palmieri; Carlos Franco; Alberto Cepeda; Aniello Anastasio. A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica. Foods 2021, 10, 1372 .

AMA Style

Rossella Festa, Rosa Ambrosio, Alexandre Lamas, Lorena Gratino, Gianna Palmieri, Carlos Franco, Alberto Cepeda, Aniello Anastasio. A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica. Foods. 2021; 10 (6):1372.

Chicago/Turabian Style

Rossella Festa; Rosa Ambrosio; Alexandre Lamas; Lorena Gratino; Gianna Palmieri; Carlos Franco; Alberto Cepeda; Aniello Anastasio. 2021. "A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica." Foods 10, no. 6: 1372.

Review article
Published: 18 May 2021 in Current Opinion in Food Science
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In food authentication research, species identification was first developed by comparing the sequences of several mitochondrial genes isolated from food with NCBI or Barcode of Life Database (BOLD) data. These methods usually use Sanger methodology for sequencing although they have difficulty in identifying mixed species in processed foods. The first research proposing the use of next-generation sequencing (NGS) for identification of fish for food consumption appeared in 2012. Recently, several platforms used for NGS have shown their capacity to identify up to 15 different fish species or more in a single highly processed fish product.

ACS Style

Carlos Manuel Franco; Rosa Luisa Ambrosio; Alberto Cepeda; Aniello Anastasio. Fish intended for human consumption: from DNA barcoding to a next-generation sequencing (NGS)-based approach. Current Opinion in Food Science 2021, 42, 86 -92.

AMA Style

Carlos Manuel Franco, Rosa Luisa Ambrosio, Alberto Cepeda, Aniello Anastasio. Fish intended for human consumption: from DNA barcoding to a next-generation sequencing (NGS)-based approach. Current Opinion in Food Science. 2021; 42 ():86-92.

Chicago/Turabian Style

Carlos Manuel Franco; Rosa Luisa Ambrosio; Alberto Cepeda; Aniello Anastasio. 2021. "Fish intended for human consumption: from DNA barcoding to a next-generation sequencing (NGS)-based approach." Current Opinion in Food Science 42, no. : 86-92.

Journal article
Published: 11 May 2021 in Animals
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Water-bath stunning represents the most-applied stunning system in poultry slaughtering, but within the European Union, specific indications on electric parameters that should be used, such as voltage, are missing. The objective of this study was to evaluate the efficiency of two commercially available types of electrical equipment (A and B) on broilers with different live body weights and the influence of the tested parameters on meat quality. Experimental trials in a European Union-approved slaughterhouse were carried out using two different stunners. 6600 broilers, divided into three weight groups, were stunned applying different protocols based on the same current frequencies and intensity but different voltages. The state of unconsciousness (presence of corneal reflex and wings flapping) and post-mortem defects (pectoral hemorrhages and dark meat) were evaluated by blinded trained operators. The presence of corneal reflex and petechiae were the most reported consciousness signs and post-mortem injuries, respectively. Different weights played an important role within stunner A, registering statistical differences (p < 0.01) among groups. Considering injuries, an inverse relationship between body weight and lesions was found. The results highlighted the effectiveness of both stunning systems applying the best combination of electrical parameters considering the weight of the animal and ensuring its well-being.

ACS Style

Giorgio Smaldone; Stefano Capezzuto; Rosa Ambrosio; Maria Peruzy; Raffaele Marrone; Giacomo Peres; Aniello Anastasio. The Influence of Broilers’ Body Weight on the Efficiency of Electrical Stunning and Meat Quality under Field Conditions. Animals 2021, 11, 1362 .

AMA Style

Giorgio Smaldone, Stefano Capezzuto, Rosa Ambrosio, Maria Peruzy, Raffaele Marrone, Giacomo Peres, Aniello Anastasio. The Influence of Broilers’ Body Weight on the Efficiency of Electrical Stunning and Meat Quality under Field Conditions. Animals. 2021; 11 (5):1362.

Chicago/Turabian Style

Giorgio Smaldone; Stefano Capezzuto; Rosa Ambrosio; Maria Peruzy; Raffaele Marrone; Giacomo Peres; Aniello Anastasio. 2021. "The Influence of Broilers’ Body Weight on the Efficiency of Electrical Stunning and Meat Quality under Field Conditions." Animals 11, no. 5: 1362.

Journal article
Published: 05 October 2020 in Animals
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Sardina pilchardus and Engraulis encrasicolus are considered the principal target species for commercial fishing in Europe and are widely consumed as semipreserved products. Although they are considered shelf-stable products, if treatment is not correctly applied, their consumption may represent a public health risk in regard to anisakiasis and allergic reactions. Little is known about the prevalence of Anisakis spp. in ripened products. This study aimed to evaluate the presence of Anisakis spp. larvae in deboned, in-oil anchovy and sardine fillets marketed in the EU to assess the influence of processing techniques on the prevalence of larvae. Ninety semipreserved anchovy and sardine products deriving from the Mediterranean Sea or Atlantic Ocean were collected from different EU retailers and examined using chloropeptic digestion to evaluate the presence of larvae and identify them. Thirty nonviable Anisakid larvae—A. pegreffii (30%) and A. simplex (70%)—were found. The frequency of larvae was higher in anchovies (28.8%). The low frequency of parasites found proved that processing technologies can influence the presence of larvae in final products, but it is important that visual inspection is performed only by trained people. The sources of raw materials should be considered in the production flow chart.

ACS Style

Giorgio Smaldone; Rosa Luisa Ambrosio; Raffaele Marrone; Marina Ceruso; Aniello Anastasio. Anisakis spp. Larvae in Deboned, in-Oil Fillets Made of Anchovies (Engraulis encrasicolus) and Sardines (Sardina pilchardus) Sold in EU Retailers. Animals 2020, 10, 1807 .

AMA Style

Giorgio Smaldone, Rosa Luisa Ambrosio, Raffaele Marrone, Marina Ceruso, Aniello Anastasio. Anisakis spp. Larvae in Deboned, in-Oil Fillets Made of Anchovies (Engraulis encrasicolus) and Sardines (Sardina pilchardus) Sold in EU Retailers. Animals. 2020; 10 (10):1807.

Chicago/Turabian Style

Giorgio Smaldone; Rosa Luisa Ambrosio; Raffaele Marrone; Marina Ceruso; Aniello Anastasio. 2020. "Anisakis spp. Larvae in Deboned, in-Oil Fillets Made of Anchovies (Engraulis encrasicolus) and Sardines (Sardina pilchardus) Sold in EU Retailers." Animals 10, no. 10: 1807.

Journal article
Published: 02 October 2020 in Foods
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The commercialization of porgies or seabreams of the family Sparidae has greatly increased in the last decade, and some valuable species have become subject to seafood substitution. DNA regions currently used for fish species identification in fresh and processed products belong to the mitochondrial (mt) genes cytochrome b (Cytb), cytochrome c oxidase I (COI), 16S and 12S. However, these markers amplify for fragments with lower divergence within and between some species, failing to provide informative barcodes. We adopted comparative mitogenomics, through the analysis of complete mtDNA sequences, as a compatible approach toward studying new barcoding markers. The intent is to develop a specific and rapid assay for the identification of the common pandora Pagellus erythrinus, a sparid species frequently subject to fraudulent replacement. The genetic diversity analysis (Hamming distance, p-genetic distance, gene-by-gene sequence variability) between 16 sparid mtDNA genomes highlighted the discriminating potential of a 291 bp NAD2 gene fragment. A pair of species-specific primers were successfully designed and tested by end-point and real-time PCR, achieving amplification only in P. erythrinus among several fish species. The use of the NAD2 barcoding marker provides a rapid presence/absence method for the identification of P. erythrinus.

ACS Style

Marina Ceruso; Celestina Mascolo; Pasquale De Luca; Iolanda Venuti; Giorgio Smaldone; Elio Biffali; Aniello Anastasio; Tiziana Pepe; Paolo Sordino. A Rapid Method for the Identification of Fresh and Processed Pagellus erythrinus Species against Frauds. Foods 2020, 9, 1397 .

AMA Style

Marina Ceruso, Celestina Mascolo, Pasquale De Luca, Iolanda Venuti, Giorgio Smaldone, Elio Biffali, Aniello Anastasio, Tiziana Pepe, Paolo Sordino. A Rapid Method for the Identification of Fresh and Processed Pagellus erythrinus Species against Frauds. Foods. 2020; 9 (10):1397.

Chicago/Turabian Style

Marina Ceruso; Celestina Mascolo; Pasquale De Luca; Iolanda Venuti; Giorgio Smaldone; Elio Biffali; Aniello Anastasio; Tiziana Pepe; Paolo Sordino. 2020. "A Rapid Method for the Identification of Fresh and Processed Pagellus erythrinus Species against Frauds." Foods 9, no. 10: 1397.

Mitogenome announcement
Published: 08 June 2020 in Mitochondrial DNA Part B
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The sharpsnout seabream Diplodus puntazzo Walbaum, 1792 is a target species of small-scale fishery activities and is cage-cultured for human consumption. Nonetheless, genetic information on this species is limited. We here first sequence its complete mitochondrial genome. The sequence is composed of 16,638 base pairs, accounting for 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 2 non-coding regions (D-loop and L-origin). The overall nucleotide composition is: 27.4% A, 28.9% C, 26.9% T, and 16.8% G. Maximum likelihood analyses placed D. puntazzo close to Acanthopagrus and some Pagellus species.

ACS Style

Marina Ceruso; Iolanda Venuti; David Osca; Luigi Caputi; Aniello Anastasio; Fabio Crocetta; Paolo Sordino; Tiziana Pepe. The complete mitochondrial genome of the sharpsnout seabream Diplodus puntazzo (Perciformes: Sparidae). Mitochondrial DNA Part B 2020, 5, 2379 -2381.

AMA Style

Marina Ceruso, Iolanda Venuti, David Osca, Luigi Caputi, Aniello Anastasio, Fabio Crocetta, Paolo Sordino, Tiziana Pepe. The complete mitochondrial genome of the sharpsnout seabream Diplodus puntazzo (Perciformes: Sparidae). Mitochondrial DNA Part B. 2020; 5 (3):2379-2381.

Chicago/Turabian Style

Marina Ceruso; Iolanda Venuti; David Osca; Luigi Caputi; Aniello Anastasio; Fabio Crocetta; Paolo Sordino; Tiziana Pepe. 2020. "The complete mitochondrial genome of the sharpsnout seabream Diplodus puntazzo (Perciformes: Sparidae)." Mitochondrial DNA Part B 5, no. 3: 2379-2381.

Journal article
Published: 21 May 2020 in Animals
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We aimed to evaluate the effects of post dry ageing (PDA) period on meat colour and rheological characteristics in 16 buffalo bulls fed two different diets: with (FRS) or without (CTL) rye grass. Animals were randomly divided into two feeding groups and slaughtered at 540 ± 4.7 and 533 ± 7.0 kg of live body weight, respectively, for the CTL and FRS group. After five days post-mortem ageing (T0), Semitendinosus muscle (ST) and Longissimus muscle (LD) underwent a prolonged maturation process in a controlled meat chamber for 30 days (ST) and until 60 days (LD). After 30 days (T1), significant changes (p < 0.01) in meat colour (ΔE) in both muscles of the FRS group was recorded, while no significant change was observed in CTL group. The FRS diet had a positive effect on textural properties of ST muscle compared to CTL diet, as well as hardness, chewiness and gumminess. All qualitative characteristics improved in the first period of PDA but, whereas LD showed to keep improving, extending the post-ageing period by further 30 days, the ST becomes un-processable at 60 days. In conclusion, a combined used of fresh feeding and PDA period could enhance both tenderness and colour in animal fed FSR.

ACS Style

Raffaele Marrone; Angela Salzano; Antonio Di Francia; Lucia Vollano; Roberto Di Matteo; Anna Balestrieri; Aniello Anastasio; Carmela Maria Assunta Barone. Effects of Feeding and Maturation System on Qualitative Characteristics of Buffalo Meat (Bubalus bubalis). Animals 2020, 10, 899 .

AMA Style

Raffaele Marrone, Angela Salzano, Antonio Di Francia, Lucia Vollano, Roberto Di Matteo, Anna Balestrieri, Aniello Anastasio, Carmela Maria Assunta Barone. Effects of Feeding and Maturation System on Qualitative Characteristics of Buffalo Meat (Bubalus bubalis). Animals. 2020; 10 (5):899.

Chicago/Turabian Style

Raffaele Marrone; Angela Salzano; Antonio Di Francia; Lucia Vollano; Roberto Di Matteo; Anna Balestrieri; Aniello Anastasio; Carmela Maria Assunta Barone. 2020. "Effects of Feeding and Maturation System on Qualitative Characteristics of Buffalo Meat (Bubalus bubalis)." Animals 10, no. 5: 899.

Journal article
Published: 21 February 2020 in BMC Veterinary Research
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Background The presence of Anisakis larvae in fish represents a major public health concern. Effective risk management procedures should be applied to prevent heavily infected products from reaching the market. The aim of the study is to provide preliminary data on parasite exposure and risk classification in frozen fish products by applying a risk categorization scheme (site, abundance, density and epidemiology – SADE) and Fish Parasite Rating (FPR) method. Fish and cephalopods samples (N = 771) from 5 different FAO Atlantic areas were examined and categorized after an accurate visual inspection and a chloro-peptic digestion. Results In 25 out of 33 fish species parasite larvae were found. 10897 anisakids larvae were collected and identified to genus level. Molva dypterygia, Conger conger, Zeus faber and Aphanopus carbo were shown to be the most highly infected species. SADE and FPR scores were 1 and poor, respectively, for the referred species, because of the disseminated Anisakis infection and commercial rejection. Conclusion SADE/FPR method showed high specificity and accuracy. The information provided in this work could be used in early warning systems for the detection of parasites in fishery products and might help fishing industries in establishing management strategies for infected stocks in terms of cost saving decisions.

ACS Style

Giorgio Smaldone; Elvira Abollo; Raffaele Marrone; Cristian E. M. Bernardi; Claudia Chirollo; Aniello Anastasio; Santiago P. Del Hierro. Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas. BMC Veterinary Research 2020, 16, 1 -12.

AMA Style

Giorgio Smaldone, Elvira Abollo, Raffaele Marrone, Cristian E. M. Bernardi, Claudia Chirollo, Aniello Anastasio, Santiago P. Del Hierro. Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas. BMC Veterinary Research. 2020; 16 (1):1-12.

Chicago/Turabian Style

Giorgio Smaldone; Elvira Abollo; Raffaele Marrone; Cristian E. M. Bernardi; Claudia Chirollo; Aniello Anastasio; Santiago P. Del Hierro. 2020. "Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas." BMC Veterinary Research 16, no. 1: 1-12.

Journal article
Published: 28 January 2020 in Antibiotics
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Loranthus europaeus is a well-known and important medicinal plant, with a long history of traditional medicine use. Several studies showed that it contains many bioactive compounds with a wide range of pharmacological effects. In light of these past researches, L. europaeus were chosen to consider its potential antimicrobial action. To this aim, different protocols were performed to selectively extract protein compounds, from L. europaeus yellow fruits, and evaluate the antimicrobial activity against four phytopathogenic fungi (Aspergillus niger, Alternaria spp., Penicillium spp., Botritis cinereus) and a number of foodborne bacterial pathogens (Listeria monocytogenes, Staphylococcus aureus strains, Salmonella Typhimurium and Escherichia coli) by using serial dilutions and colony formation assays. Results evidenced no antifungal activity but a notable bactericidal efficiency of a crude protein extract against two foodborne pathogens, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values between 0.2 and 0.5 mg/mL, being S. aureus strains the most susceptible bacteria. Moreover, a strong bactericidal activity against S. aureus M7 was observed by two partially purified protein fractions of about 600 and 60 kDa molecular mass in native conditions. Therefore, these plant protein extracts could be used as natural alternative preventives to control food poisoning diseases and preserve foodstuff avoiding health hazards of chemically antimicrobial applications.

ACS Style

Rosa Luisa Ambrosio; Lorena Gratino; Sara Mirino; Ennio Cocca; Antonino Pollio; Aniello Anastasio; Gianna Palmieri; Marco Balestrieri; Angelo Genovese; Marta Gogliettino. The Bactericidal Activity of Protein Extracts from Loranthus europaeus Berries: A Natural Resource of Bioactive Compounds. Antibiotics 2020, 9, 47 .

AMA Style

Rosa Luisa Ambrosio, Lorena Gratino, Sara Mirino, Ennio Cocca, Antonino Pollio, Aniello Anastasio, Gianna Palmieri, Marco Balestrieri, Angelo Genovese, Marta Gogliettino. The Bactericidal Activity of Protein Extracts from Loranthus europaeus Berries: A Natural Resource of Bioactive Compounds. Antibiotics. 2020; 9 (2):47.

Chicago/Turabian Style

Rosa Luisa Ambrosio; Lorena Gratino; Sara Mirino; Ennio Cocca; Antonino Pollio; Aniello Anastasio; Gianna Palmieri; Marco Balestrieri; Angelo Genovese; Marta Gogliettino. 2020. "The Bactericidal Activity of Protein Extracts from Loranthus europaeus Berries: A Natural Resource of Bioactive Compounds." Antibiotics 9, no. 2: 47.

Journal article
Published: 19 July 2019 in International Journal of Environmental Research and Public Health
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To assess the quality of shellfish harvest areas, bivalve mollusk samples from three coastal areas of the Campania region in Southwest Italy were evaluated for viruses over a three-year period (2015–2017). Screening of 289 samples from shellfish farms and other locations by qPCR and RT-qPCR identified hepatitis A virus (HAV; 8.9%), norovirus GI (NoVGI; 10.8%) and GII (NoVGII; 39.7%), rotavirus (RV; 9.0%), astrovirus (AsV; 20.8%), sapovirus (SaV; 18.8%), aichivirus-1 (AiV-1; 5.6%), and adenovirus (AdV, 5.6%). Hepatitis E virus (HEV) was never detected. Sequence analysis identified HAV as genotype IA and AdV as type 41. This study demonstrates the presence of different enteric viruses within bivalve mollusks, highlighting the limitations of the current EU classification system for shellfish growing waters.

ACS Style

Giovanna Fusco; Aniello Anastasio; David H. Kingsley; Maria Grazia Amoroso; Tiziana Pepe; Pina M. Fratamico; Barbara Cioffi; Rachele Rossi; Giuseppina La Rosa; Federica Boccia. Detection of Hepatitis A Virus and Other Enteric Viruses in Shellfish Collected in the Gulf of Naples, Italy. International Journal of Environmental Research and Public Health 2019, 16, 2588 .

AMA Style

Giovanna Fusco, Aniello Anastasio, David H. Kingsley, Maria Grazia Amoroso, Tiziana Pepe, Pina M. Fratamico, Barbara Cioffi, Rachele Rossi, Giuseppina La Rosa, Federica Boccia. Detection of Hepatitis A Virus and Other Enteric Viruses in Shellfish Collected in the Gulf of Naples, Italy. International Journal of Environmental Research and Public Health. 2019; 16 (14):2588.

Chicago/Turabian Style

Giovanna Fusco; Aniello Anastasio; David H. Kingsley; Maria Grazia Amoroso; Tiziana Pepe; Pina M. Fratamico; Barbara Cioffi; Rachele Rossi; Giuseppina La Rosa; Federica Boccia. 2019. "Detection of Hepatitis A Virus and Other Enteric Viruses in Shellfish Collected in the Gulf of Naples, Italy." International Journal of Environmental Research and Public Health 16, no. 14: 2588.

Journal article
Published: 29 June 2019 in Molecules
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Octopus vulgaris constitute an important part of most suitable marine resources for human consumption, however, they can represent a source in chemical contaminants intake such as heavy metals. In this scenario, the aim of the study was the evaluation of the concentration of lead (Pb), cadmium (Cd) and mercury (Hg) in the muscle and digestive gland of octopus caught from two different locations along Campania coast (Castellammare di Stabia and Napoli) and the estimation of their weekly human intake derived from the ingestion of octopus. Analysing 38 samples showed a higher concentration of Pb in the muscle of octopus in Castellammare di Stabia than in Napoli. No statistical differences were reported for Cd, Pb and Hg concentrations in the digestive gland of octopus between two sampling sites. Differences were observed between the two tissue types, with a higher level of Cd and Pb observed in the digestive gland compared with the muscle. Noteworthy, the consumption of muscle from Castellammare di Stabia could increase Pb intake in heavy consumers of local octopus. In conclusion, the present work determines that it is important to improve strategies to minimize environmental pollution sources in these areas.

ACS Style

Andrea Ariano; Raffaele Marrone; Rebecca Andreini; Giorgio Smaldone; Salvatore Velotto; Serena Montagnaro; Aniello Anastasio; Lorella Severino. Metal Concentration in Muscle and Digestive Gland of Common Octopus (Octopus vulgaris) from Two Coastal Site in Southern Tyrrhenian Sea (Italy). Molecules 2019, 24, 2401 .

AMA Style

Andrea Ariano, Raffaele Marrone, Rebecca Andreini, Giorgio Smaldone, Salvatore Velotto, Serena Montagnaro, Aniello Anastasio, Lorella Severino. Metal Concentration in Muscle and Digestive Gland of Common Octopus (Octopus vulgaris) from Two Coastal Site in Southern Tyrrhenian Sea (Italy). Molecules. 2019; 24 (13):2401.

Chicago/Turabian Style

Andrea Ariano; Raffaele Marrone; Rebecca Andreini; Giorgio Smaldone; Salvatore Velotto; Serena Montagnaro; Aniello Anastasio; Lorella Severino. 2019. "Metal Concentration in Muscle and Digestive Gland of Common Octopus (Octopus vulgaris) from Two Coastal Site in Southern Tyrrhenian Sea (Italy)." Molecules 24, no. 13: 2401.

Journal article
Published: 08 March 2019 in Chemosphere
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Fast Detection Strategy for Cyanobacterial blooms and associated Cyanotoxins (FDSCC) is a multidisciplinary strategy that allows early detection, in 24 man-hours, of cyanobacteria and related cyanotoxins in water and bivalve samples. This approach combines the advantages of remote/proximal sensing with those of analytical/bioinformatics analyses, namely, LC-HRMS-based molecular networking. The detection of Lyngbyatoxin A, a lipophilic cyanotoxin, in all analyzed water samples and in bivalves, commonly used as food, was the proof of the reliability of the new method.

ACS Style

Germana Esposito; Roberta Teta; Raffaele Marrone; Carlo De Sterlich; Marco Casazza; Aniello Anastasio; Massimiliano Lega; Valeria Costantino. A Fast Detection Strategy for Cyanobacterial blooms and associated cyanotoxins (FDSCC) reveals the occurrence of lyngbyatoxin A in campania (South Italy). Chemosphere 2019, 225, 342 -351.

AMA Style

Germana Esposito, Roberta Teta, Raffaele Marrone, Carlo De Sterlich, Marco Casazza, Aniello Anastasio, Massimiliano Lega, Valeria Costantino. A Fast Detection Strategy for Cyanobacterial blooms and associated cyanotoxins (FDSCC) reveals the occurrence of lyngbyatoxin A in campania (South Italy). Chemosphere. 2019; 225 ():342-351.

Chicago/Turabian Style

Germana Esposito; Roberta Teta; Raffaele Marrone; Carlo De Sterlich; Marco Casazza; Aniello Anastasio; Massimiliano Lega; Valeria Costantino. 2019. "A Fast Detection Strategy for Cyanobacterial blooms and associated cyanotoxins (FDSCC) reveals the occurrence of lyngbyatoxin A in campania (South Italy)." Chemosphere 225, no. : 342-351.

Journal article
Published: 31 January 2019 in Molecules
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The distribution of pyrethroid insecticide deltamethrin (DLM) in goat milk and cheese (caciotta) following pour-on administration at the sheep dosage (DLMS-10 mL/60 kg body weight) and double dosage (DLMD-20 mL/60 kg body weight) was studied. DLM concentrations were measured in milk collected from study animals (No.14) before treatment and at 2, 4, 8, 12, 16, 24, 30, 36, 48, 56, until 168 h (7 days) post treatment and in caciotta cheese at 12 and 24 h post treatment. At both dosages, the maximum level of DLM residues in goat milk and cheese was below the maximum residue limit (MRL) of 20 μg kg−1 established for bovine milk (EU No 37/2010) at all time points. However, in terms of public health, higher DLM residues in cheese show that further specific studies should be performed on double dosage efficacy and pharmacokinetic and pharmacodynamics properties of ectoparasites in lactating goats.

ACS Style

Raffaele Marrone; Abilasha Ramkumar; Giorgio Smaldone; Domenico Rufrano; Claudia Chirollo; Vincenzo Veneziano; Martin Danaher; Aniello Anastasio. Deltamethrin Residues in Milk and Cheese of Lactating Goats (Capra hircus). Molecules 2019, 24, 517 .

AMA Style

Raffaele Marrone, Abilasha Ramkumar, Giorgio Smaldone, Domenico Rufrano, Claudia Chirollo, Vincenzo Veneziano, Martin Danaher, Aniello Anastasio. Deltamethrin Residues in Milk and Cheese of Lactating Goats (Capra hircus). Molecules. 2019; 24 (3):517.

Chicago/Turabian Style

Raffaele Marrone; Abilasha Ramkumar; Giorgio Smaldone; Domenico Rufrano; Claudia Chirollo; Vincenzo Veneziano; Martin Danaher; Aniello Anastasio. 2019. "Deltamethrin Residues in Milk and Cheese of Lactating Goats (Capra hircus)." Molecules 24, no. 3: 517.

Mitogenome announcement
Published: 02 January 2019 in Mitochondrial DNA Part B
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The complete mitochondrial genome of the Angolan dentex (Dentex angolensis, Poll and Maul, 1953) is 16.581 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes. The overall base composition of D. angolesis mtDNA is: 27.4% for A, 28% for C, 16.7% for G, 27.9% for T. Phylogenetic analysis indicated that D. angolensis is most closely related to D. tumifrons. Whole genome sequencing of D. angolensis mitochondrial DNA will contribute to improving knowledge about Sparidae evolution.

ACS Style

Celestina Mascolo; Marina Ceruso; Claudia Chirollo; Giuseppe Palma; Aniello Anastasio; Paolo Sordino; Tiziana Pepe. The complete mitochondrial genome of the Angolan dentex Dentex angolensis (Perciformes: Sparidae). Mitochondrial DNA Part B 2019, 4, 1245 -1246.

AMA Style

Celestina Mascolo, Marina Ceruso, Claudia Chirollo, Giuseppe Palma, Aniello Anastasio, Paolo Sordino, Tiziana Pepe. The complete mitochondrial genome of the Angolan dentex Dentex angolensis (Perciformes: Sparidae). Mitochondrial DNA Part B. 2019; 4 (1):1245-1246.

Chicago/Turabian Style

Celestina Mascolo; Marina Ceruso; Claudia Chirollo; Giuseppe Palma; Aniello Anastasio; Paolo Sordino; Tiziana Pepe. 2019. "The complete mitochondrial genome of the Angolan dentex Dentex angolensis (Perciformes: Sparidae)." Mitochondrial DNA Part B 4, no. 1: 1245-1246.

Journal article
Published: 01 August 2018 in International Journal of Food Microbiology
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Antimicrobial peptides have received great attention for their potential benefits to extend the shelf-life of food-products. Innate defense regulator peptide-1018 (IDR-1018) represents a promising candidate for such applications, due to its broad-spectrum antimicrobial activity, although food-isolated pathogens have been poorly investigated. Herein, we describe the design and the structural-functional characterization of a new 1018-derivative peptide named 1018-K6, in which the alanine in position 6 was replaced with a lysine. Spectroscopic analysis revealed a noticeable switch from β-sheet to helical conformations of 1018-K6 respect to IDR-1018, with a faster folding kinetic and increased structural stability. Moreover, 1018-K6 evidenced a significant antibiofilm/bactericidal efficiency specifically against Listeria monocytogenes isolates from food-products and food-processing environments, belonging to serotype 4b involved in the majority of human-listeriosis cases, with EC50 values two- five-fold lower than those measured for IDR-1018. Therefore, a single amino-acid substitution in IDR-1018 sequence produced severe changes in peptide conformation and antimicrobial performances.

ACS Style

Gianna Palmieri; Marco Balestrieri; Federico Capuano; Yolande T.R. Proroga; Francesco Pomilio; Patrizia Centorame; Alessia Riccio; Raffaele Marrone; Aniello Anastasio. Bactericidal and antibiofilm activity of bactenecin-derivative peptides against the food-pathogen Listeria monocytogenes : New perspectives for food processing industry. International Journal of Food Microbiology 2018, 279, 33 -42.

AMA Style

Gianna Palmieri, Marco Balestrieri, Federico Capuano, Yolande T.R. Proroga, Francesco Pomilio, Patrizia Centorame, Alessia Riccio, Raffaele Marrone, Aniello Anastasio. Bactericidal and antibiofilm activity of bactenecin-derivative peptides against the food-pathogen Listeria monocytogenes : New perspectives for food processing industry. International Journal of Food Microbiology. 2018; 279 ():33-42.

Chicago/Turabian Style

Gianna Palmieri; Marco Balestrieri; Federico Capuano; Yolande T.R. Proroga; Francesco Pomilio; Patrizia Centorame; Alessia Riccio; Raffaele Marrone; Aniello Anastasio. 2018. "Bactericidal and antibiofilm activity of bactenecin-derivative peptides against the food-pathogen Listeria monocytogenes : New perspectives for food processing industry." International Journal of Food Microbiology 279, no. : 33-42.

Original articles
Published: 01 January 2018 in CyTA - Journal of Food
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The levels of PCDDs, PCDFs and DL-PCBs were analyzed both in milk and adipose tissues sampled “in vivo” from lactating, drying off and heifer buffaloes from a Campania farm which had been impounded by the competent authority owing to the high dioxin levels found in bulk milk. The chemical determination was carried out by HRGC-HRMS using US EPA Method 1613b. The range of WHO-TEQ values for the PCDDs/PCDFs in adipose tissues was 1.79 to 68.64 pg g−1 fat and in milk was 8.33 to 13.95 pg g−1 fat. The contamination profile for dioxins and furans was given by 1,2,3,7,8-PeCDD; 2,3,4,7,8-PeCDF; 1,2,3,6,7,8-HxCDD and 2,3,7,8-TCDD. The levels of DL-PCBs in adipose tissue varied from 1.38 to 20.13 pg g−1 fat while ranged from 8.33 to 13.95 pg g−1 fat in milk. The pattern of DL-PCBs in both matrices was dominated by congeners PCB 126 and PCB 169. Graphical Abstract

ACS Style

Claudia Chirollo; Marina Ceruso; Tiziana Pepe; Antonio Vassallo; Raffaele Marrone; Lorella Severino; Aniello Anastasio. Levels and congeners distribution of dioxins, furans and dioxin-like PCBs in buffaloes adipose tissues sampled in vivo and milk. CyTA - Journal of Food 2018, 16, 1109 -1114.

AMA Style

Claudia Chirollo, Marina Ceruso, Tiziana Pepe, Antonio Vassallo, Raffaele Marrone, Lorella Severino, Aniello Anastasio. Levels and congeners distribution of dioxins, furans and dioxin-like PCBs in buffaloes adipose tissues sampled in vivo and milk. CyTA - Journal of Food. 2018; 16 (1):1109-1114.

Chicago/Turabian Style

Claudia Chirollo; Marina Ceruso; Tiziana Pepe; Antonio Vassallo; Raffaele Marrone; Lorella Severino; Aniello Anastasio. 2018. "Levels and congeners distribution of dioxins, furans and dioxin-like PCBs in buffaloes adipose tissues sampled in vivo and milk." CyTA - Journal of Food 16, no. 1: 1109-1114.

Journal article
Published: 01 November 2016 in Food Chemistry
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Recently there has been growing interest in the discovery of new antimicrobial agents to increase safety and shelf-life of food products. Here, we developed an innovative approach by introducing the concept that mitochondrial targeting peptides (MTP) can interact and disrupt bacterial membranes, acting as antimicrobial agents. As proof-of-principle, we used a multidisciplinary strategy by combining in silico predictions, docking simulations and antimicrobial assays, to identify two peptides, MTP1 and MTP2, which were structurally and functionally characterized. Both compounds appeared effective against Listeria monocytogenes, one of the most important foodborne pathogens. Specifically, a significant bactericidal activity was evidenced with EC50 values of 16.8 ± 1.2 μM for MTP1 and 109 ± 7.0 μM for MTP2. Finally, NMR structure determinations suggested that MTP1 would be oriented into the membrane bilayer, while the molecular shape of MTP2 could indicate porin-mediated antimicrobial mechanisms, as predicted using molecular docking analysis. Therefore, MTPs represent alternative sources to design new potential bio-preservatives.

ACS Style

Gianna Palmieri; Marco Balestrieri; Yolande T.R. Proroga; Lucia Falcigno; Angelo Facchiano; Alessia Riccio; Federico Capuano; Raffaele Marrone; Gianluca Neglia; Aniello Anastasio. New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence. Food Chemistry 2016, 211, 546 -554.

AMA Style

Gianna Palmieri, Marco Balestrieri, Yolande T.R. Proroga, Lucia Falcigno, Angelo Facchiano, Alessia Riccio, Federico Capuano, Raffaele Marrone, Gianluca Neglia, Aniello Anastasio. New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence. Food Chemistry. 2016; 211 ():546-554.

Chicago/Turabian Style

Gianna Palmieri; Marco Balestrieri; Yolande T.R. Proroga; Lucia Falcigno; Angelo Facchiano; Alessia Riccio; Federico Capuano; Raffaele Marrone; Gianluca Neglia; Aniello Anastasio. 2016. "New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence." Food Chemistry 211, no. : 546-554.

Journal article
Published: 01 November 2014 in Journal of Food Composition and Analysis
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ACS Style

Raffaele Marrone; Anna Balestrieri; Tiziana Pepe; Lucia Vollano; Nicoletta Murru; Michael J. D’Occhio; Aniello Anastasio. Physicochemical composition, fatty acid profile and cholesterol content of “Pecorino Carmasciano” cheese, a traditional Italian dairy product. Journal of Food Composition and Analysis 2014, 36, 85 -89.

AMA Style

Raffaele Marrone, Anna Balestrieri, Tiziana Pepe, Lucia Vollano, Nicoletta Murru, Michael J. D’Occhio, Aniello Anastasio. Physicochemical composition, fatty acid profile and cholesterol content of “Pecorino Carmasciano” cheese, a traditional Italian dairy product. Journal of Food Composition and Analysis. 2014; 36 (1-2):85-89.

Chicago/Turabian Style

Raffaele Marrone; Anna Balestrieri; Tiziana Pepe; Lucia Vollano; Nicoletta Murru; Michael J. D’Occhio; Aniello Anastasio. 2014. "Physicochemical composition, fatty acid profile and cholesterol content of “Pecorino Carmasciano” cheese, a traditional Italian dairy product." Journal of Food Composition and Analysis 36, no. 1-2: 85-89.

Journal article
Published: 23 January 2014 in Environmental Science and Pollution Research
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Nitrate and nitrite are toxicants that have become increasingly significant environmental chemicals. Increase in environmental distribution of nitrogenous compounds, especially in surface and ground water, has been attributed to the intensive use of nitrate as agricultural fertilizers and to increasing amounts of nitrogenous wastes produced by municipalities, industries, and feedlots. The purpose of this study is to illustrate a fatal nitrate toxicosis in cattle associated with the consumption of fennels (Foeniculum vulgare). Fifteen cows from the same farm suddenly developed weakness, muscular tremors, respiratory distress, and finally convulsions. The affected animals died within 24 to 48 h from the onset of the clinical signs. Five cows underwent a complete post-mortem examination. In all examined animals, gross lesions included presence of dark unclotted blood around the nostrils and the anal region, moderate inflammation of the gastrointestinal mucosa, and brown discoloration of the skeletal muscles and kidneys. The histological examination showed tubular degeneration and congestion of glomerular vessels in the kidney. Toxicological analysis detected nitrates at 4 672.2 ppm in the fennels used to feed the animals. The source of exposure to nitrates was identified in the fennels. The fennels were grown in a polluted area of the Campania region in southern Italy and distributed in a public market for human consumption. The waste from the sale of the fennels was fed to the cows. The accumulation of nitrates in some vegetables poses a risk not only for animal health but also for human and environmental safety

ACS Style

Alessandro Costagliola; Franco Roperto; Domenico Benedetto; Aniello Anastasio; Raffaele Marrone; Antonella Perillo; Valeria Russo; Serenella Papparella; Orlando Paciello. Outbreak of fatal nitrate toxicosis associated with consumption of fennels (Foeniculum vulgare) in cattle farmed in Campania region (southern Italy). Environmental Science and Pollution Research 2014, 21, 6252 -6257.

AMA Style

Alessandro Costagliola, Franco Roperto, Domenico Benedetto, Aniello Anastasio, Raffaele Marrone, Antonella Perillo, Valeria Russo, Serenella Papparella, Orlando Paciello. Outbreak of fatal nitrate toxicosis associated with consumption of fennels (Foeniculum vulgare) in cattle farmed in Campania region (southern Italy). Environmental Science and Pollution Research. 2014; 21 (9):6252-6257.

Chicago/Turabian Style

Alessandro Costagliola; Franco Roperto; Domenico Benedetto; Aniello Anastasio; Raffaele Marrone; Antonella Perillo; Valeria Russo; Serenella Papparella; Orlando Paciello. 2014. "Outbreak of fatal nitrate toxicosis associated with consumption of fennels (Foeniculum vulgare) in cattle farmed in Campania region (southern Italy)." Environmental Science and Pollution Research 21, no. 9: 6252-6257.

Journal article
Published: 01 January 2014 in BMC Veterinary Research
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Environmental contamination by pharmaceuticals is a public health concern: drugs administered to humans and animals are excreted with urine or faeces and attend the sewage treatment. The main consequences of use and abuse of antibiotics is the development and diffusion of antibiotic resistance that has become a serious global problem. Aim of the study is to evaluate the presence of antimicrobial residues and to assess the antimicrobial resistance in bacteria species isolated from different wild caught seawater fish and fishery products.

ACS Style

Giorgio Smaldone; Raffaele Marrone; Silvia Cappiello; Giuseppe A Martin; Gaetano Oliva; Maria L Cortesi; Aniello Anastasio. Occurrence of antibiotic resistance in bacteria isolated from seawater organisms caught in Campania Region: preliminary study. BMC Veterinary Research 2014, 10, 161 -161.

AMA Style

Giorgio Smaldone, Raffaele Marrone, Silvia Cappiello, Giuseppe A Martin, Gaetano Oliva, Maria L Cortesi, Aniello Anastasio. Occurrence of antibiotic resistance in bacteria isolated from seawater organisms caught in Campania Region: preliminary study. BMC Veterinary Research. 2014; 10 (1):161-161.

Chicago/Turabian Style

Giorgio Smaldone; Raffaele Marrone; Silvia Cappiello; Giuseppe A Martin; Gaetano Oliva; Maria L Cortesi; Aniello Anastasio. 2014. "Occurrence of antibiotic resistance in bacteria isolated from seawater organisms caught in Campania Region: preliminary study." BMC Veterinary Research 10, no. 1: 161-161.