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Prof. Dr. Raffaele Marrone
Department of Veterinary Medicine and Animal Production (MVPA), University of Naples “Federico II”, 80137 Naples, Italy

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0 Dairy Science
0 Food Chemistry
0 Food Processing
0 Food Safety
0 Nutrition

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food processing and engineering

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Journal article
Published: 11 May 2021 in Animals
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Water-bath stunning represents the most-applied stunning system in poultry slaughtering, but within the European Union, specific indications on electric parameters that should be used, such as voltage, are missing. The objective of this study was to evaluate the efficiency of two commercially available types of electrical equipment (A and B) on broilers with different live body weights and the influence of the tested parameters on meat quality. Experimental trials in a European Union-approved slaughterhouse were carried out using two different stunners. 6600 broilers, divided into three weight groups, were stunned applying different protocols based on the same current frequencies and intensity but different voltages. The state of unconsciousness (presence of corneal reflex and wings flapping) and post-mortem defects (pectoral hemorrhages and dark meat) were evaluated by blinded trained operators. The presence of corneal reflex and petechiae were the most reported consciousness signs and post-mortem injuries, respectively. Different weights played an important role within stunner A, registering statistical differences (p < 0.01) among groups. Considering injuries, an inverse relationship between body weight and lesions was found. The results highlighted the effectiveness of both stunning systems applying the best combination of electrical parameters considering the weight of the animal and ensuring its well-being.

ACS Style

Giorgio Smaldone; Stefano Capezzuto; Rosa Ambrosio; Maria Peruzy; Raffaele Marrone; Giacomo Peres; Aniello Anastasio. The Influence of Broilers’ Body Weight on the Efficiency of Electrical Stunning and Meat Quality under Field Conditions. Animals 2021, 11, 1362 .

AMA Style

Giorgio Smaldone, Stefano Capezzuto, Rosa Ambrosio, Maria Peruzy, Raffaele Marrone, Giacomo Peres, Aniello Anastasio. The Influence of Broilers’ Body Weight on the Efficiency of Electrical Stunning and Meat Quality under Field Conditions. Animals. 2021; 11 (5):1362.

Chicago/Turabian Style

Giorgio Smaldone; Stefano Capezzuto; Rosa Ambrosio; Maria Peruzy; Raffaele Marrone; Giacomo Peres; Aniello Anastasio. 2021. "The Influence of Broilers’ Body Weight on the Efficiency of Electrical Stunning and Meat Quality under Field Conditions." Animals 11, no. 5: 1362.

Journal article
Published: 20 April 2021 in Veterinary Sciences
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Recently, several concerns have been expressed on red meat quality and consumption. The aims of this study were to evaluate the influence of different breeding techniques and a prolonged post dry aging (PDA) maturation process on biomolecules level in raw buffalo meat. In the first experiment, two groups of animals were maintained with different space availability (15 vs. 10 m2/animal) for 90 days and biomolecules content was evaluated. In experiment 2, two diets (with or without ryegrass green forage) were used to assess the concentration of these biomolecules. Finally, in experiment 3, the meat of the animals that showed the highest content of biomolecules was chosen to assess the influence of the PDA maturation process. Buffaloes reared at 15 m2 showed a significantly (p < 0.05) higher content of the considered biomolecules compared with their counterparts. Similarly, buffaloes fed green forage showed higher content of biomolecules (p < 0.05) compared with the control group. The meat of the animals bred at 15 m2 and fed green forage showed a significant (p < 0.01) increase of biomolecules content during the PDA maturation process up to 60 days without influence microbiological profile in terms of total aerobic bacterial counts, yeasts, and molds. In conclusion, breeding techniques and PDA maturation system could enhance biomolecules levels in terms of quality, without affect health standards.

ACS Style

Angela Salzano; Alessio Cotticelli; Raffaele Marrone; Michael D’Occhio; Nunzia D’Onofrio; Gianluca Neglia; Rosa Ambrosio; Maria Balestrieri; Giuseppe Campanile. Effect of Breeding Techniques and Prolonged Post Dry Aging Maturation Process on Biomolecule Levels in Raw Buffalo Meat. Veterinary Sciences 2021, 8, 66 .

AMA Style

Angela Salzano, Alessio Cotticelli, Raffaele Marrone, Michael D’Occhio, Nunzia D’Onofrio, Gianluca Neglia, Rosa Ambrosio, Maria Balestrieri, Giuseppe Campanile. Effect of Breeding Techniques and Prolonged Post Dry Aging Maturation Process on Biomolecule Levels in Raw Buffalo Meat. Veterinary Sciences. 2021; 8 (4):66.

Chicago/Turabian Style

Angela Salzano; Alessio Cotticelli; Raffaele Marrone; Michael D’Occhio; Nunzia D’Onofrio; Gianluca Neglia; Rosa Ambrosio; Maria Balestrieri; Giuseppe Campanile. 2021. "Effect of Breeding Techniques and Prolonged Post Dry Aging Maturation Process on Biomolecule Levels in Raw Buffalo Meat." Veterinary Sciences 8, no. 4: 66.

Journal article
Published: 19 November 2020 in Food Chemistry
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The effect of green feed on health-promoting biomolecules in milk was examined in dairy buffaloes. Buffaloes received a total mixed ration (TMR) (Control, C; n = 40) or TMR + alfalfa green feed (30% of diet) (Treated, T; n = 40). Biomolecules and functional activity were measured in milk obtained twice-monthly. Treated buffaloes had higher milk l-carnitine, acetyl-l-carnitine, propionyl-l-carnitine and δ-valerobetaine (P < 0.01). They also had higher antioxidant activity (P < 0.01). Compared with C buffaloes, milk of T buffaloes improved the viability of endothelial cells exposed to high-glucose (P < 0.01), and reduced intracellular lipid peroxidation, reactive oxygen species (ROS), and cytokine release (P < 0.01). Milk of T buffaloes inhibited with greater potency the viability of human HCT116 and Cal 27 cancer cells (P < 0.001). The findings show that including green feed in the diet of dairy buffaloes enhances health-promoting biomolecules and the antioxidant and antineoplastic properties of milk.

ACS Style

Angela Salzano; Gianluca Neglia; Nunzia D'Onofrio; Maria Luisa Balestrieri; Antonio Limone; Alessio Cotticelli; Raffaele Marrone; Aniello Anastasio; Michael J. D'Occhio; Giuseppe Campanile. Green feed increases antioxidant and antineoplastic activity of buffalo milk: A globally significant livestock. Food Chemistry 2020, 344, 128669 .

AMA Style

Angela Salzano, Gianluca Neglia, Nunzia D'Onofrio, Maria Luisa Balestrieri, Antonio Limone, Alessio Cotticelli, Raffaele Marrone, Aniello Anastasio, Michael J. D'Occhio, Giuseppe Campanile. Green feed increases antioxidant and antineoplastic activity of buffalo milk: A globally significant livestock. Food Chemistry. 2020; 344 ():128669.

Chicago/Turabian Style

Angela Salzano; Gianluca Neglia; Nunzia D'Onofrio; Maria Luisa Balestrieri; Antonio Limone; Alessio Cotticelli; Raffaele Marrone; Aniello Anastasio; Michael J. D'Occhio; Giuseppe Campanile. 2020. "Green feed increases antioxidant and antineoplastic activity of buffalo milk: A globally significant livestock." Food Chemistry 344, no. : 128669.

Journal article
Published: 05 October 2020 in Animals
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Sardina pilchardus and Engraulis encrasicolus are considered the principal target species for commercial fishing in Europe and are widely consumed as semipreserved products. Although they are considered shelf-stable products, if treatment is not correctly applied, their consumption may represent a public health risk in regard to anisakiasis and allergic reactions. Little is known about the prevalence of Anisakis spp. in ripened products. This study aimed to evaluate the presence of Anisakis spp. larvae in deboned, in-oil anchovy and sardine fillets marketed in the EU to assess the influence of processing techniques on the prevalence of larvae. Ninety semipreserved anchovy and sardine products deriving from the Mediterranean Sea or Atlantic Ocean were collected from different EU retailers and examined using chloropeptic digestion to evaluate the presence of larvae and identify them. Thirty nonviable Anisakid larvae—A. pegreffii (30%) and A. simplex (70%)—were found. The frequency of larvae was higher in anchovies (28.8%). The low frequency of parasites found proved that processing technologies can influence the presence of larvae in final products, but it is important that visual inspection is performed only by trained people. The sources of raw materials should be considered in the production flow chart.

ACS Style

Giorgio Smaldone; Rosa Luisa Ambrosio; Raffaele Marrone; Marina Ceruso; Aniello Anastasio. Anisakis spp. Larvae in Deboned, in-Oil Fillets Made of Anchovies (Engraulis encrasicolus) and Sardines (Sardina pilchardus) Sold in EU Retailers. Animals 2020, 10, 1807 .

AMA Style

Giorgio Smaldone, Rosa Luisa Ambrosio, Raffaele Marrone, Marina Ceruso, Aniello Anastasio. Anisakis spp. Larvae in Deboned, in-Oil Fillets Made of Anchovies (Engraulis encrasicolus) and Sardines (Sardina pilchardus) Sold in EU Retailers. Animals. 2020; 10 (10):1807.

Chicago/Turabian Style

Giorgio Smaldone; Rosa Luisa Ambrosio; Raffaele Marrone; Marina Ceruso; Aniello Anastasio. 2020. "Anisakis spp. Larvae in Deboned, in-Oil Fillets Made of Anchovies (Engraulis encrasicolus) and Sardines (Sardina pilchardus) Sold in EU Retailers." Animals 10, no. 10: 1807.

Journal article
Published: 21 May 2020 in Animals
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We aimed to evaluate the effects of post dry ageing (PDA) period on meat colour and rheological characteristics in 16 buffalo bulls fed two different diets: with (FRS) or without (CTL) rye grass. Animals were randomly divided into two feeding groups and slaughtered at 540 ± 4.7 and 533 ± 7.0 kg of live body weight, respectively, for the CTL and FRS group. After five days post-mortem ageing (T0), Semitendinosus muscle (ST) and Longissimus muscle (LD) underwent a prolonged maturation process in a controlled meat chamber for 30 days (ST) and until 60 days (LD). After 30 days (T1), significant changes (p < 0.01) in meat colour (ΔE) in both muscles of the FRS group was recorded, while no significant change was observed in CTL group. The FRS diet had a positive effect on textural properties of ST muscle compared to CTL diet, as well as hardness, chewiness and gumminess. All qualitative characteristics improved in the first period of PDA but, whereas LD showed to keep improving, extending the post-ageing period by further 30 days, the ST becomes un-processable at 60 days. In conclusion, a combined used of fresh feeding and PDA period could enhance both tenderness and colour in animal fed FSR.

ACS Style

Raffaele Marrone; Angela Salzano; Antonio Di Francia; Lucia Vollano; Roberto Di Matteo; Anna Balestrieri; Aniello Anastasio; Carmela Maria Assunta Barone. Effects of Feeding and Maturation System on Qualitative Characteristics of Buffalo Meat (Bubalus bubalis). Animals 2020, 10, 899 .

AMA Style

Raffaele Marrone, Angela Salzano, Antonio Di Francia, Lucia Vollano, Roberto Di Matteo, Anna Balestrieri, Aniello Anastasio, Carmela Maria Assunta Barone. Effects of Feeding and Maturation System on Qualitative Characteristics of Buffalo Meat (Bubalus bubalis). Animals. 2020; 10 (5):899.

Chicago/Turabian Style

Raffaele Marrone; Angela Salzano; Antonio Di Francia; Lucia Vollano; Roberto Di Matteo; Anna Balestrieri; Aniello Anastasio; Carmela Maria Assunta Barone. 2020. "Effects of Feeding and Maturation System on Qualitative Characteristics of Buffalo Meat (Bubalus bubalis)." Animals 10, no. 5: 899.

Journal article
Published: 21 February 2020 in BMC Veterinary Research
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Background The presence of Anisakis larvae in fish represents a major public health concern. Effective risk management procedures should be applied to prevent heavily infected products from reaching the market. The aim of the study is to provide preliminary data on parasite exposure and risk classification in frozen fish products by applying a risk categorization scheme (site, abundance, density and epidemiology – SADE) and Fish Parasite Rating (FPR) method. Fish and cephalopods samples (N = 771) from 5 different FAO Atlantic areas were examined and categorized after an accurate visual inspection and a chloro-peptic digestion. Results In 25 out of 33 fish species parasite larvae were found. 10897 anisakids larvae were collected and identified to genus level. Molva dypterygia, Conger conger, Zeus faber and Aphanopus carbo were shown to be the most highly infected species. SADE and FPR scores were 1 and poor, respectively, for the referred species, because of the disseminated Anisakis infection and commercial rejection. Conclusion SADE/FPR method showed high specificity and accuracy. The information provided in this work could be used in early warning systems for the detection of parasites in fishery products and might help fishing industries in establishing management strategies for infected stocks in terms of cost saving decisions.

ACS Style

Giorgio Smaldone; Elvira Abollo; Raffaele Marrone; Cristian E. M. Bernardi; Claudia Chirollo; Aniello Anastasio; Santiago P. Del Hierro. Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas. BMC Veterinary Research 2020, 16, 1 -12.

AMA Style

Giorgio Smaldone, Elvira Abollo, Raffaele Marrone, Cristian E. M. Bernardi, Claudia Chirollo, Aniello Anastasio, Santiago P. Del Hierro. Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas. BMC Veterinary Research. 2020; 16 (1):1-12.

Chicago/Turabian Style

Giorgio Smaldone; Elvira Abollo; Raffaele Marrone; Cristian E. M. Bernardi; Claudia Chirollo; Aniello Anastasio; Santiago P. Del Hierro. 2020. "Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas." BMC Veterinary Research 16, no. 1: 1-12.

Journal article
Published: 29 June 2019 in Molecules
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Octopus vulgaris constitute an important part of most suitable marine resources for human consumption, however, they can represent a source in chemical contaminants intake such as heavy metals. In this scenario, the aim of the study was the evaluation of the concentration of lead (Pb), cadmium (Cd) and mercury (Hg) in the muscle and digestive gland of octopus caught from two different locations along Campania coast (Castellammare di Stabia and Napoli) and the estimation of their weekly human intake derived from the ingestion of octopus. Analysing 38 samples showed a higher concentration of Pb in the muscle of octopus in Castellammare di Stabia than in Napoli. No statistical differences were reported for Cd, Pb and Hg concentrations in the digestive gland of octopus between two sampling sites. Differences were observed between the two tissue types, with a higher level of Cd and Pb observed in the digestive gland compared with the muscle. Noteworthy, the consumption of muscle from Castellammare di Stabia could increase Pb intake in heavy consumers of local octopus. In conclusion, the present work determines that it is important to improve strategies to minimize environmental pollution sources in these areas.

ACS Style

Andrea Ariano; Raffaele Marrone; Rebecca Andreini; Giorgio Smaldone; Salvatore Velotto; Serena Montagnaro; Aniello Anastasio; Lorella Severino. Metal Concentration in Muscle and Digestive Gland of Common Octopus (Octopus vulgaris) from Two Coastal Site in Southern Tyrrhenian Sea (Italy). Molecules 2019, 24, 2401 .

AMA Style

Andrea Ariano, Raffaele Marrone, Rebecca Andreini, Giorgio Smaldone, Salvatore Velotto, Serena Montagnaro, Aniello Anastasio, Lorella Severino. Metal Concentration in Muscle and Digestive Gland of Common Octopus (Octopus vulgaris) from Two Coastal Site in Southern Tyrrhenian Sea (Italy). Molecules. 2019; 24 (13):2401.

Chicago/Turabian Style

Andrea Ariano; Raffaele Marrone; Rebecca Andreini; Giorgio Smaldone; Salvatore Velotto; Serena Montagnaro; Aniello Anastasio; Lorella Severino. 2019. "Metal Concentration in Muscle and Digestive Gland of Common Octopus (Octopus vulgaris) from Two Coastal Site in Southern Tyrrhenian Sea (Italy)." Molecules 24, no. 13: 2401.

Journal article
Published: 08 March 2019 in Chemosphere
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Fast Detection Strategy for Cyanobacterial blooms and associated Cyanotoxins (FDSCC) is a multidisciplinary strategy that allows early detection, in 24 man-hours, of cyanobacteria and related cyanotoxins in water and bivalve samples. This approach combines the advantages of remote/proximal sensing with those of analytical/bioinformatics analyses, namely, LC-HRMS-based molecular networking. The detection of Lyngbyatoxin A, a lipophilic cyanotoxin, in all analyzed water samples and in bivalves, commonly used as food, was the proof of the reliability of the new method.

ACS Style

Germana Esposito; Roberta Teta; Raffaele Marrone; Carlo De Sterlich; Marco Casazza; Aniello Anastasio; Massimiliano Lega; Valeria Costantino. A Fast Detection Strategy for Cyanobacterial blooms and associated cyanotoxins (FDSCC) reveals the occurrence of lyngbyatoxin A in campania (South Italy). Chemosphere 2019, 225, 342 -351.

AMA Style

Germana Esposito, Roberta Teta, Raffaele Marrone, Carlo De Sterlich, Marco Casazza, Aniello Anastasio, Massimiliano Lega, Valeria Costantino. A Fast Detection Strategy for Cyanobacterial blooms and associated cyanotoxins (FDSCC) reveals the occurrence of lyngbyatoxin A in campania (South Italy). Chemosphere. 2019; 225 ():342-351.

Chicago/Turabian Style

Germana Esposito; Roberta Teta; Raffaele Marrone; Carlo De Sterlich; Marco Casazza; Aniello Anastasio; Massimiliano Lega; Valeria Costantino. 2019. "A Fast Detection Strategy for Cyanobacterial blooms and associated cyanotoxins (FDSCC) reveals the occurrence of lyngbyatoxin A in campania (South Italy)." Chemosphere 225, no. : 342-351.

Journal article
Published: 31 January 2019 in Molecules
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The distribution of pyrethroid insecticide deltamethrin (DLM) in goat milk and cheese (caciotta) following pour-on administration at the sheep dosage (DLMS-10 mL/60 kg body weight) and double dosage (DLMD-20 mL/60 kg body weight) was studied. DLM concentrations were measured in milk collected from study animals (No.14) before treatment and at 2, 4, 8, 12, 16, 24, 30, 36, 48, 56, until 168 h (7 days) post treatment and in caciotta cheese at 12 and 24 h post treatment. At both dosages, the maximum level of DLM residues in goat milk and cheese was below the maximum residue limit (MRL) of 20 μg kg−1 established for bovine milk (EU No 37/2010) at all time points. However, in terms of public health, higher DLM residues in cheese show that further specific studies should be performed on double dosage efficacy and pharmacokinetic and pharmacodynamics properties of ectoparasites in lactating goats.

ACS Style

Raffaele Marrone; Abilasha Ramkumar; Giorgio Smaldone; Domenico Rufrano; Claudia Chirollo; Vincenzo Veneziano; Martin Danaher; Aniello Anastasio. Deltamethrin Residues in Milk and Cheese of Lactating Goats (Capra hircus). Molecules 2019, 24, 517 .

AMA Style

Raffaele Marrone, Abilasha Ramkumar, Giorgio Smaldone, Domenico Rufrano, Claudia Chirollo, Vincenzo Veneziano, Martin Danaher, Aniello Anastasio. Deltamethrin Residues in Milk and Cheese of Lactating Goats (Capra hircus). Molecules. 2019; 24 (3):517.

Chicago/Turabian Style

Raffaele Marrone; Abilasha Ramkumar; Giorgio Smaldone; Domenico Rufrano; Claudia Chirollo; Vincenzo Veneziano; Martin Danaher; Aniello Anastasio. 2019. "Deltamethrin Residues in Milk and Cheese of Lactating Goats (Capra hircus)." Molecules 24, no. 3: 517.

Journal article
Published: 01 August 2018 in International Journal of Food Microbiology
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Antimicrobial peptides have received great attention for their potential benefits to extend the shelf-life of food-products. Innate defense regulator peptide-1018 (IDR-1018) represents a promising candidate for such applications, due to its broad-spectrum antimicrobial activity, although food-isolated pathogens have been poorly investigated. Herein, we describe the design and the structural-functional characterization of a new 1018-derivative peptide named 1018-K6, in which the alanine in position 6 was replaced with a lysine. Spectroscopic analysis revealed a noticeable switch from β-sheet to helical conformations of 1018-K6 respect to IDR-1018, with a faster folding kinetic and increased structural stability. Moreover, 1018-K6 evidenced a significant antibiofilm/bactericidal efficiency specifically against Listeria monocytogenes isolates from food-products and food-processing environments, belonging to serotype 4b involved in the majority of human-listeriosis cases, with EC50 values two- five-fold lower than those measured for IDR-1018. Therefore, a single amino-acid substitution in IDR-1018 sequence produced severe changes in peptide conformation and antimicrobial performances.

ACS Style

Gianna Palmieri; Marco Balestrieri; Federico Capuano; Yolande T.R. Proroga; Francesco Pomilio; Patrizia Centorame; Alessia Riccio; Raffaele Marrone; Aniello Anastasio. Bactericidal and antibiofilm activity of bactenecin-derivative peptides against the food-pathogen Listeria monocytogenes : New perspectives for food processing industry. International Journal of Food Microbiology 2018, 279, 33 -42.

AMA Style

Gianna Palmieri, Marco Balestrieri, Federico Capuano, Yolande T.R. Proroga, Francesco Pomilio, Patrizia Centorame, Alessia Riccio, Raffaele Marrone, Aniello Anastasio. Bactericidal and antibiofilm activity of bactenecin-derivative peptides against the food-pathogen Listeria monocytogenes : New perspectives for food processing industry. International Journal of Food Microbiology. 2018; 279 ():33-42.

Chicago/Turabian Style

Gianna Palmieri; Marco Balestrieri; Federico Capuano; Yolande T.R. Proroga; Francesco Pomilio; Patrizia Centorame; Alessia Riccio; Raffaele Marrone; Aniello Anastasio. 2018. "Bactericidal and antibiofilm activity of bactenecin-derivative peptides against the food-pathogen Listeria monocytogenes : New perspectives for food processing industry." International Journal of Food Microbiology 279, no. : 33-42.

Original articles
Published: 01 January 2018 in CyTA - Journal of Food
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The levels of PCDDs, PCDFs and DL-PCBs were analyzed both in milk and adipose tissues sampled “in vivo” from lactating, drying off and heifer buffaloes from a Campania farm which had been impounded by the competent authority owing to the high dioxin levels found in bulk milk. The chemical determination was carried out by HRGC-HRMS using US EPA Method 1613b. The range of WHO-TEQ values for the PCDDs/PCDFs in adipose tissues was 1.79 to 68.64 pg g−1 fat and in milk was 8.33 to 13.95 pg g−1 fat. The contamination profile for dioxins and furans was given by 1,2,3,7,8-PeCDD; 2,3,4,7,8-PeCDF; 1,2,3,6,7,8-HxCDD and 2,3,7,8-TCDD. The levels of DL-PCBs in adipose tissue varied from 1.38 to 20.13 pg g−1 fat while ranged from 8.33 to 13.95 pg g−1 fat in milk. The pattern of DL-PCBs in both matrices was dominated by congeners PCB 126 and PCB 169. Graphical Abstract

ACS Style

Claudia Chirollo; Marina Ceruso; Tiziana Pepe; Antonio Vassallo; Raffaele Marrone; Lorella Severino; Aniello Anastasio. Levels and congeners distribution of dioxins, furans and dioxin-like PCBs in buffaloes adipose tissues sampled in vivo and milk. CyTA - Journal of Food 2018, 16, 1109 -1114.

AMA Style

Claudia Chirollo, Marina Ceruso, Tiziana Pepe, Antonio Vassallo, Raffaele Marrone, Lorella Severino, Aniello Anastasio. Levels and congeners distribution of dioxins, furans and dioxin-like PCBs in buffaloes adipose tissues sampled in vivo and milk. CyTA - Journal of Food. 2018; 16 (1):1109-1114.

Chicago/Turabian Style

Claudia Chirollo; Marina Ceruso; Tiziana Pepe; Antonio Vassallo; Raffaele Marrone; Lorella Severino; Aniello Anastasio. 2018. "Levels and congeners distribution of dioxins, furans and dioxin-like PCBs in buffaloes adipose tissues sampled in vivo and milk." CyTA - Journal of Food 16, no. 1: 1109-1114.

Journal article
Published: 20 October 2017 in Italian Journal of Food Safety
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The European Food Safety Authority stated that many traditional marinating and cold smoking methods are not sufficient to kill A. simplex and asked to evaluate alternative treatments for killing viable parasites in fishery. Baccalà is a well-liked traditional product. The aim of study was to evaluate the effectiveness of the salting process on the inactivation of nematodes of the genus Anisakis in naturally infected Baccalà fillets. N. 19 fillets, subjected to a dual salting process (brine and dry salting) were analyzed. Visual inspection and chloropeptic digestion were performed. Larvae viability was evaluated, and parameters such as NaCl (%), moisture (%), WPS and aw were determined. In n. 17 samples parasites were found 123 parasites with a mean intensity of 7.23±4.78 and an mean abundance of 6.47±5.05. Visual examination has revealed 109 parasites. 61.8% of larvae were found in the ventral portions. The results show that salting process with a salt concentration of 18.6%, aw values of 0.7514 and 24.15% WPS in all parts of baccalà fillets, devitalise Anisakidae larvae in a 15-day period.

ACS Style

Giorgio Smaldone; Raffaele Marrone; Giuseppe Palma; Paolo Sarnelli; Aniello Anastasio. Preliminary study on the inactivation of anisakid larvae in baccalà prepared according to traditional methods. Italian Journal of Food Safety 2017, 6, 6964 .

AMA Style

Giorgio Smaldone, Raffaele Marrone, Giuseppe Palma, Paolo Sarnelli, Aniello Anastasio. Preliminary study on the inactivation of anisakid larvae in baccalà prepared according to traditional methods. Italian Journal of Food Safety. 2017; 6 (4):6964.

Chicago/Turabian Style

Giorgio Smaldone; Raffaele Marrone; Giuseppe Palma; Paolo Sarnelli; Aniello Anastasio. 2017. "Preliminary study on the inactivation of anisakid larvae in baccalà prepared according to traditional methods." Italian Journal of Food Safety 6, no. 4: 6964.

Journal article
Published: 16 August 2017 in Italian Journal of Food Safety
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Listeria monocytogenes (L.m.) is an agent of serious foodborne illness. It is a major concern for the food industry, since microorganism, growing in biofilms is protected against cleaning and disinfection and is difficult to eradicate. Aim of this study was to develop a protocol to assess the ability of two flexible packaging materials, named HGP40 and GND35, submitted to corona discharge treatment, to limit the production of L.m. biofilm at 12±1°C. Two strains were selected for this study: L.m ATCC 7644 and L. m. EURL 12MOB098LM isolated from dairy products. Results suggest that both L.m. strains were able to form biofilm on packaging materials tested. The differences on HGP40 and GND35s treated and not treated surfaces were not statistically significant.

ACS Style

Patrizia Centorame; Anna Rita D'Angelo; Federica Di Simone; Romolo Salini; Alessandra Cornacchia; Raffaele Marrone; Aniello Anastasio; Francesco Pomilio. Listeria monocytogenes biofilm production on food packaging materials submitted to physical treatment. Italian Journal of Food Safety 2017, 6, 6654 -6654.

AMA Style

Patrizia Centorame, Anna Rita D'Angelo, Federica Di Simone, Romolo Salini, Alessandra Cornacchia, Raffaele Marrone, Aniello Anastasio, Francesco Pomilio. Listeria monocytogenes biofilm production on food packaging materials submitted to physical treatment. Italian Journal of Food Safety. 2017; 6 (3):6654-6654.

Chicago/Turabian Style

Patrizia Centorame; Anna Rita D'Angelo; Federica Di Simone; Romolo Salini; Alessandra Cornacchia; Raffaele Marrone; Aniello Anastasio; Francesco Pomilio. 2017. "Listeria monocytogenes biofilm production on food packaging materials submitted to physical treatment." Italian Journal of Food Safety 6, no. 3: 6654-6654.

Journal article
Published: 24 January 2017 in Italian Journal of Food Safety
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Consumer is very careful about healthiness; in this context nutritionists often highlight the importance of fish for human nutrition because of their protein and fatty acid composition. In order to stimulate utilisation and consumption of fish species by unusual target groups such as children, the aim of this research was to formulate and to evaluate shelf-life and nutritional values of fish preparations stored in modified atmosphere packaging (MAP). Fish species used for trail were Trachurus trachurus and Oncorhynchus mykiss fished and farmed in Basilicata region respectively. Fish burgers were made with different ingredients of plant and animal origin and packed in air (control) and in MAP and stored at refrigeration atemperature. Sensory, physicalchemical analysis as pH, aw, total volatile nitrogen (TVN), trimetilammine (TMA), thiobarbituric acid (TBA), free fatty acids (FFA) and microbiological analysis like aerobic plate count, Enterobacteriaceae, Escherichia coli, Pseudomonas spp., sulphite-reducing clostridia, Staphylococci, Salmonella spp. and Listeria monocytogenes were performed at intervals of 0°, 1°, 2°, 5°, 8°, 15°, 22°, day from production. Results showed that fish burgers stored in MAP had a longer shelf-life; protein degradation indexes and spoilage bacterial species showed lower values in the samples packaged in MAP compared with the control. The formulation of the fish burger meets the approval of the target consumers. The mixing of natural ingredients has made possible both the enhancement of the organoleptic characteristics with an excellent balance of nutritional values. The diversification of fish preparations, besides enhancing the fish production of marginal areas would add value to a product with potential and remarkable profit margins.

ACS Style

Giorgio Smaldone; Raffaele Marrone; Tiziana Zottola; Lucia Vollano; Giulio Grossi; Maria Luisa Cortesi. Formulation and shelf-life of fish burgers served to preschool children. Italian Journal of Food Safety 2017, 6, 6373 .

AMA Style

Giorgio Smaldone, Raffaele Marrone, Tiziana Zottola, Lucia Vollano, Giulio Grossi, Maria Luisa Cortesi. Formulation and shelf-life of fish burgers served to preschool children. Italian Journal of Food Safety. 2017; 6 (1):6373.

Chicago/Turabian Style

Giorgio Smaldone; Raffaele Marrone; Tiziana Zottola; Lucia Vollano; Giulio Grossi; Maria Luisa Cortesi. 2017. "Formulation and shelf-life of fish burgers served to preschool children." Italian Journal of Food Safety 6, no. 1: 6373.

Journal article
Published: 01 November 2016 in Food Chemistry
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Recently there has been growing interest in the discovery of new antimicrobial agents to increase safety and shelf-life of food products. Here, we developed an innovative approach by introducing the concept that mitochondrial targeting peptides (MTP) can interact and disrupt bacterial membranes, acting as antimicrobial agents. As proof-of-principle, we used a multidisciplinary strategy by combining in silico predictions, docking simulations and antimicrobial assays, to identify two peptides, MTP1 and MTP2, which were structurally and functionally characterized. Both compounds appeared effective against Listeria monocytogenes, one of the most important foodborne pathogens. Specifically, a significant bactericidal activity was evidenced with EC50 values of 16.8 ± 1.2 μM for MTP1 and 109 ± 7.0 μM for MTP2. Finally, NMR structure determinations suggested that MTP1 would be oriented into the membrane bilayer, while the molecular shape of MTP2 could indicate porin-mediated antimicrobial mechanisms, as predicted using molecular docking analysis. Therefore, MTPs represent alternative sources to design new potential bio-preservatives.

ACS Style

Gianna Palmieri; Marco Balestrieri; Yolande T.R. Proroga; Lucia Falcigno; Angelo Facchiano; Alessia Riccio; Federico Capuano; Raffaele Marrone; Gianluca Neglia; Aniello Anastasio. New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence. Food Chemistry 2016, 211, 546 -554.

AMA Style

Gianna Palmieri, Marco Balestrieri, Yolande T.R. Proroga, Lucia Falcigno, Angelo Facchiano, Alessia Riccio, Federico Capuano, Raffaele Marrone, Gianluca Neglia, Aniello Anastasio. New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence. Food Chemistry. 2016; 211 ():546-554.

Chicago/Turabian Style

Gianna Palmieri; Marco Balestrieri; Yolande T.R. Proroga; Lucia Falcigno; Angelo Facchiano; Alessia Riccio; Federico Capuano; Raffaele Marrone; Gianluca Neglia; Aniello Anastasio. 2016. "New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence." Food Chemistry 211, no. : 546-554.

Journal article
Published: 20 September 2016 in Theriogenology
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The aim of this study was to evaluate the effect of carnitine supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was cryopreserved in BioXcell containing 0 (control group), 2.5 and 7.5-mM carnitine. After thawing, viability, motility, membrane integrity and capacitation status (assessed by localization of phosphotyrosine-containing proteins and chlortetracycline, chlortetracycline assay) were evaluated. Furthermore, total antioxidant capacity, reactive oxygen species (ROS) and lipid peroxidation levels, as well as adenosine triphosphate (ATP) content and phospholipids concentration were assessed. Finally, in vitro–fertilizing ability was evaluated after heterologous IVF. An increased post-thawing sperm motility and membrane integrity were recorded in both treated groups compared with the control (44.4 ± 3.5, 53.1 ± 3.9, and 52.5 ± 3.6%; P < 0.05 and 48.44 ± 0.69, 55.19 ± 0.54, 59.63 ± 0.30%; P < 0.01 with 0, 2.5-mM, and 7.5-mM carnitine, respectively). Supplementation of carnitine to the freezing extender decreased (P < 0.01) the percentage of sperm displaying fluorescence at both equatorial and anterior acrosomal regions (pattern EA), corresponding to high capacitation level, compared with the control (30.3 ± 3.8, 18.8 ± 2.8, and 7.2 ± 2.9%, respectively, with 0, 2.5-mM, and 7.5-mM carnitine). In agreement with this, carnitine also decreased (P < 0.01) the percentage of sperm displaying chlortetracycline pattern B (capacitated sperm) (63.8 ± 1.8, 46.8 ± 2.2, and 37.2 ± 1.8%, respectively with 0, 2.5-, and 7.5-mM carnitine). Interestingly, carnitine increased total antioxidant capacity and ATP content of buffalo frozen-thawed sperm (1.32 ± 0.02, 1.34 ± 0.01, 1.37 ± 0.01 mM/L and 4.1 ± 0.1, 5.3 ± 0.1 and 8.2 ± 0.4 nM × 108 sperm; P < 0.01, respectively, with 0, 2.5- and 7.5-mM carnitine). Intracellular ROS decreased in carnitine-treated sperm compared with the control, as indicated by dihydroethidium (DHE) values (0.22 ± 0.01, 0.18 ± 0.01, and 0.14 ± 0.0 μM/100 μL dihydroethidium, respectively, with 0, 2.5-, and 7.5-mM carnitine; P < 0.01), whereas lipid peroxidation levels (on average 30.5 ± 0.3 nmol/mL MDA) and phospholipids concentration (on average 0.14 ± 0.00 μg/120 × 106 sperm) were unaffected. Despite the improved sperm quality, the percentage of normospermic penetration after IVF was not influenced (on average 53.5 ± 1.8). In conclusion, enrichment of extender with carnitine improved buffalo sperm quality by increasing ATP generation and modulating ROS production, without affecting in vitro fertilizing ability.

ACS Style

Valentina Longobardi; Angela Salzano; Giuseppe Campanile; Raffaele Marrone; Francesco Palumbo; Milena Vitiello; Gianluigi Zullo; Bianca Gasparrini. Carnitine supplementation decreases capacitation-like changes of frozen-thawed buffalo spermatozoa. Theriogenology 2016, 88, 236 -243.

AMA Style

Valentina Longobardi, Angela Salzano, Giuseppe Campanile, Raffaele Marrone, Francesco Palumbo, Milena Vitiello, Gianluigi Zullo, Bianca Gasparrini. Carnitine supplementation decreases capacitation-like changes of frozen-thawed buffalo spermatozoa. Theriogenology. 2016; 88 ():236-243.

Chicago/Turabian Style

Valentina Longobardi; Angela Salzano; Giuseppe Campanile; Raffaele Marrone; Francesco Palumbo; Milena Vitiello; Gianluigi Zullo; Bianca Gasparrini. 2016. "Carnitine supplementation decreases capacitation-like changes of frozen-thawed buffalo spermatozoa." Theriogenology 88, no. : 236-243.

Data article
Published: 16 June 2016 in Data in Brief
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Here we report details on the design and conformational analysis of two novel peptides showing antimicrobial properties, as reported in the research article, “New antimicrobial peptides against foodborne pathogens: from in silico design to experimental evidence” G. Palmieri, M. Balestrieri, Y.T.R. Proroga, L. Falcigno, A. Facchiano, A. Riccio, F. Capuano, R. Marrone, G. Campanile, A. Anastasio (2016) [1]. NMR data, such as chemical shifts in two different solvents as well as aCH protons deviations from random coil values and NOE patterns, are shown together with the statistics of structural calculations. Strategy and particulars of molecular design are presented.

ACS Style

Lucia Falcigno; Gianna Palmieri; Marco Balestrieri; Yolande T.R. Proroga; Angelo Facchiano; Alessia Riccio; Federico Capuano; Raffaele Marrone; Giuseppe Campanile; Aniello Anastasio. NMR and computational data of two novel antimicrobial peptides. Data in Brief 2016, 8, 562 -569.

AMA Style

Lucia Falcigno, Gianna Palmieri, Marco Balestrieri, Yolande T.R. Proroga, Angelo Facchiano, Alessia Riccio, Federico Capuano, Raffaele Marrone, Giuseppe Campanile, Aniello Anastasio. NMR and computational data of two novel antimicrobial peptides. Data in Brief. 2016; 8 ():562-569.

Chicago/Turabian Style

Lucia Falcigno; Gianna Palmieri; Marco Balestrieri; Yolande T.R. Proroga; Angelo Facchiano; Alessia Riccio; Federico Capuano; Raffaele Marrone; Giuseppe Campanile; Aniello Anastasio. 2016. "NMR and computational data of two novel antimicrobial peptides." Data in Brief 8, no. : 562-569.

Journal article
Published: 01 June 2016 in Food Control
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Aniello Anastasio; Giorgio Smaldone; Domenico Cacace; Raffaele Marrone; Andrea Lo Voi; Mario Santoro; Giuseppe Cringoli; Edoardo Pozio. Inactivation of Anisakis pegreffii larvae in anchovies (Engraulis encrasicolus) by salting and quality assessment of finished product. Food Control 2016, 64, 115 -119.

AMA Style

Aniello Anastasio, Giorgio Smaldone, Domenico Cacace, Raffaele Marrone, Andrea Lo Voi, Mario Santoro, Giuseppe Cringoli, Edoardo Pozio. Inactivation of Anisakis pegreffii larvae in anchovies (Engraulis encrasicolus) by salting and quality assessment of finished product. Food Control. 2016; 64 ():115-119.

Chicago/Turabian Style

Aniello Anastasio; Giorgio Smaldone; Domenico Cacace; Raffaele Marrone; Andrea Lo Voi; Mario Santoro; Giuseppe Cringoli; Edoardo Pozio. 2016. "Inactivation of Anisakis pegreffii larvae in anchovies (Engraulis encrasicolus) by salting and quality assessment of finished product." Food Control 64, no. : 115-119.

Journal article
Published: 01 May 2016 in Poultry Science
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The effects of different amounts and frequencies of stunning sine wave alternating current were investigated under field conditions. Seven hundred and fifty broilers were stunned in an electrical water bath with an average root mean square (RMS) current of 150, 200, and 250 mA and frequencies of 200, 400, 600, 800, and 1,200 Hz. The occurrence of corneal reflex, spontaneous eye blinking, and a positive response to a painful stimulus were monitored and recorded immediately after the stunning and at 20 s post-stun. Statistical analysis showed that the electrical stunning frequency (P = 0.0004), the stunning RMS current (P < 0.0001) and the interaction between stunning frequency and stunning current (P < 0.0001) had a significant effect on the occurrence of animals experiencing an abolition of corneal reflex at 20 s post-stun.

ACS Style

M. Girasole; Raffaele Marrone; A. Anastasio; Antonio Chianese; Raffaelina Mercogliano; M. L. Cortesi. Effect of electrical water bath stunning on physical reflexes of broilers: evaluation of stunning efficacy under field conditions. Poultry Science 2016, 95, 1205 -1210.

AMA Style

M. Girasole, Raffaele Marrone, A. Anastasio, Antonio Chianese, Raffaelina Mercogliano, M. L. Cortesi. Effect of electrical water bath stunning on physical reflexes of broilers: evaluation of stunning efficacy under field conditions. Poultry Science. 2016; 95 (5):1205-1210.

Chicago/Turabian Style

M. Girasole; Raffaele Marrone; A. Anastasio; Antonio Chianese; Raffaelina Mercogliano; M. L. Cortesi. 2016. "Effect of electrical water bath stunning on physical reflexes of broilers: evaluation of stunning efficacy under field conditions." Poultry Science 95, no. 5: 1205-1210.

Journal article
Published: 01 January 2016 in Virus Research
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In this study, we investigated hepatitis E virus (HEV) infection in piglets sampled in two farms in southern Italy. The virus was detected in 11 out of 15 animals tested. Based on sequence analysis, the 6 Italian strains examined belonged to two clusters containing both swine and human strains of either genotype 3 subtype e or f from Europe and Japan. The two Italian strain clusters shared nucleotide identity of 81.8% and 87.5% in the ORF2 (capsid protein) and ORF1 (RdRp) diagnostic fragments, respectively, confirming the heterogeneity of genotype 3 viruses circulating in pigs in Italy. The complete genome of one genotype 3 subtype e strain and the full ORF2 and ORF3 coding regions of one of the genotype 3f strains, obtained in this study, were compared to other HEV sequences available on line (NCBI database). The results of analysis showed that porcine strains clustered together with human and swine strains detected in Europe. Most changes in the coding region corresponded to synonymous mutations, and only the ORF3 showed a positive selection. Further, analyses are needed to understand the clinical significance of HEV genotypes and subtypes.

ACS Style

Ilaria Di Bartolo; Giorgia Angeloni; Marina Monini; Ester Maione; Raffaele Marrone; Fabio Ostanello; Franco Maria Ruggeri. Complete genome sequencing of a genotype 3 hepatitis E virus strain identified in a swine farm in Italy. Virus Research 2016, 211, 89 -95.

AMA Style

Ilaria Di Bartolo, Giorgia Angeloni, Marina Monini, Ester Maione, Raffaele Marrone, Fabio Ostanello, Franco Maria Ruggeri. Complete genome sequencing of a genotype 3 hepatitis E virus strain identified in a swine farm in Italy. Virus Research. 2016; 211 ():89-95.

Chicago/Turabian Style

Ilaria Di Bartolo; Giorgia Angeloni; Marina Monini; Ester Maione; Raffaele Marrone; Fabio Ostanello; Franco Maria Ruggeri. 2016. "Complete genome sequencing of a genotype 3 hepatitis E virus strain identified in a swine farm in Italy." Virus Research 211, no. : 89-95.