Mercury content and fatty acids in muscles of Perca fluviatilis L. (European perch), Leuciscus idus L. (ide), Cyprinus carpio L. (European or common carp), Oncorhynchus mykiss Walb. (rainbow trout), Platichthys flesus L. (European flounder). and Clupea harengus L. (bream) from the Polish market were investigated. The total mercury was processed with AAS. The fatty acids were analyzed by gas chromatography. The concentration of mercury in muscles varied from 0.006 to 0.138 mg/kg and decreased as follows: perch ≈ ide > flounder > herring ≈ bream ≈ rainbow trout > carp (p ≤ 0.05). There were only significant positive correlations between body weight and mercury content in muscle tissue of carp (r = 0.878), flounder (r = 0.925) and herring (r = 0.982) (p ≤ 0.05). The atherogenic index (AI), thrombogenicity index (TI) and flesh-lipid quality index (FLQ) were calculated as follows 0.33–0.70 (IA), 0.16–0.31 (IT) and 13.01–33.22 (FLQ). Hypocholesterolemic (OFA) and hypercholesterolemic fatty acids (DFA) in muscles of fish ranged from 18.26 to 23.01 and from 73.91 to 78.46, respectively. In most cases, there were not significant correlations between size (body weight and total length) and fatty acids in the muscles of the examined fish (p > 0.05). The Target Hazard Quotient (THQ) values were below 1, which shows that there is no non-carcinogenic health risk to the consumer by consuming the examined fish.
The African catfish is one of the most promising warm-freshwater species for aquaculture development in the upcoming years, although there are two primary limitations in its production: less-than-desirable survival rates and low larvae quality. In this study, a novel method of egg fertilization for this species was evaluated which has already been successfully utilized in the production of other finfish. The results indicate that dividing the semen designated for fertilization of eggs into smaller aliquots and adding it to the eggs at 30 s intervals (i.e., 0, 30 and 60 s), after activation of the eggs with water, increased the hatching rate percentage (97.1%) compared to control groups (87.9%) in which the entire portion of semen was added to eggs at the time of water addition for egg activation. Results with repeated evaluations in the field confirmed that eggs with greater biological quality were fertilized at a similar rate using the modified and control methods for fertilization, although when the eggs were of a lesser biological quality, the fertilization rate was greater using the modified methods than the control methods.
The zebrafish is a freshwater fish of the Cyprinidae family, which is frequently used in scientific research. It owes its popularity to its genome, whose structure is comparable to the human genome and, for this reason, this species is often used in human medical research. However, such research requires high-quality material to conduct tests producing repeatable results. This study examines the effect of providing feed enriched with essential fatty acids, especially docosahexaenoic acid, to zebrafish spawners on fertility as well as the survival rate and growth of their offspring. The experiments revealed a significant (p < 0.05) effect of feeding spawners with feed enriched with polyunsaturated fatty acids on all tested parameters: their fertility (30% higher compared to the control group), survival rate, and the posthatching size of larvae, which were larger by 13% and 4%, respectively, compared to the control group.