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Bioprocess specialist, interested in the production and characterization of polysaccharides, in particular agave fructans and also microbial polysaccharides.
In the present study, a novel laccase from ascomycete Gliomastix murorum was produced in agro-industrial wastes and entrapped in galactomannan beads for Reactive Blue 2 (Rb-2) decolorization. The maximum laccase production in agave bagasse-based medium occurred at 72 h (1798.6 UL−1). Entrapped laccase decolorized ˃80% of 0.5 mM Rb-2 in 2 h without the addition of redox mediator. Km for Rb-2 substrate was 1.42 mM, with a Vmax of 1.19 µmol min−1 for entrapped laccase. Galactomannan matrices produce stability to acid pH (2–5) and temperatures from 20–70 °C. Reusability assays showed that entrapped laccase could retain efficient Rb-2 decolorization of ˃80% six times. In general, galactomannan used for entrapment of laccase provides economic advantages in large-scale wastewater treatment due to its natural origin and efficient results.
Itzel C. Romero-Soto; Raúl B. Martínez-Pérez; Jorge A. Rodríguez; Rosa M. Camacho-Ruiz; Alejandra Barbachano-Torres; Martha Martín del Campo; Juan Napoles-Armenta; Jorge E. Pliego-Sandoval; María O. Concha-Guzmán; María Angeles Camacho-Ruiz. Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization. Sustainability 2021, 13, 9019 .
AMA StyleItzel C. Romero-Soto, Raúl B. Martínez-Pérez, Jorge A. Rodríguez, Rosa M. Camacho-Ruiz, Alejandra Barbachano-Torres, Martha Martín del Campo, Juan Napoles-Armenta, Jorge E. Pliego-Sandoval, María O. Concha-Guzmán, María Angeles Camacho-Ruiz. Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization. Sustainability. 2021; 13 (16):9019.
Chicago/Turabian StyleItzel C. Romero-Soto; Raúl B. Martínez-Pérez; Jorge A. Rodríguez; Rosa M. Camacho-Ruiz; Alejandra Barbachano-Torres; Martha Martín del Campo; Juan Napoles-Armenta; Jorge E. Pliego-Sandoval; María O. Concha-Guzmán; María Angeles Camacho-Ruiz. 2021. "Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization." Sustainability 13, no. 16: 9019.
The isolation and molecular and chemo-taxonomic identification of seventeen halophilic archaea from the Santa Bárbara saltern, Sonora, México, were performed. Eight strains were selected based on pigmentation. Molecular identification revealed that the strains belonged to the Haloarcula, Halolamina and Halorubrum genera. Neutral lipids (quinones) were identified in all strains. Glycolipid S-DGD was found only in Halolamina sp. strain M3; polar phospholipids 2,3-O-phytanyl-sn-glycerol-1-phosphoryl-3-sn-glycerol (PG), 2,3-di-O-phytanyl-sn-glycero-1-phospho-3′-sn-glycerol-1′-methyl phosphate (PGP-Me) and sodium salt 1-(3-sn-phosphatidyl)-rac-glycerol were found in all the strains; and one unidentified glyco-phospholipid in strains M1, M3 and M4. Strains M1, M3 and M5 were selected for further studies based on carotenoid production. The effect of glucose and succinic and glutamic acid on carotenoid production was assessed. In particular, carotenoid production and growth significantly improved in the presence of glucose in strains Haloarcula sp. M1 and Halorubrum sp. M5 but not in Halolamina sp. M3. Glutamic and succinic acid had no effect on carotenoid production, and even was negative for Halorubrum sp. M5. Growth was increased by glutamic and succinic acid on Haloarcula sp. M1 but not in the other strains. This work describes for first time the presence of halophilic archaea in the Santa Bárbara saltern and highlights the differences in the effect of carbon sources on the growth and carotenoid production of haloarchaea.
Ana Vázquez-Madrigal; Alejandra Barbachano-Torres; Melchor Arellano-Plaza; Manuel Kirchmayr; Ilaria Finore; Annarita Poli; Barbara Nicolaus; Susana De La Torre Zavala; Rosa Camacho-Ruiz. Effect of Carbon Sources in Carotenoid Production from Haloarcula sp. M1, Halolamina sp. M3 and Halorubrum sp. M5, Halophilic Archaea Isolated from Sonora Saltern, Mexico. Microorganisms 2021, 9, 1096 .
AMA StyleAna Vázquez-Madrigal, Alejandra Barbachano-Torres, Melchor Arellano-Plaza, Manuel Kirchmayr, Ilaria Finore, Annarita Poli, Barbara Nicolaus, Susana De La Torre Zavala, Rosa Camacho-Ruiz. Effect of Carbon Sources in Carotenoid Production from Haloarcula sp. M1, Halolamina sp. M3 and Halorubrum sp. M5, Halophilic Archaea Isolated from Sonora Saltern, Mexico. Microorganisms. 2021; 9 (5):1096.
Chicago/Turabian StyleAna Vázquez-Madrigal; Alejandra Barbachano-Torres; Melchor Arellano-Plaza; Manuel Kirchmayr; Ilaria Finore; Annarita Poli; Barbara Nicolaus; Susana De La Torre Zavala; Rosa Camacho-Ruiz. 2021. "Effect of Carbon Sources in Carotenoid Production from Haloarcula sp. M1, Halolamina sp. M3 and Halorubrum sp. M5, Halophilic Archaea Isolated from Sonora Saltern, Mexico." Microorganisms 9, no. 5: 1096.
In this study, a low-cost chemically defined (CD) culture medium was proposed and evaluated with the aim of replacing culture media such as yeast mold (YM) and yeast peptone dextrose (YPD), commonly used for growth and carotenoid production by Xanthophyllomyces dendrorhous. Initially, the CD culture medium was compared to the YM and YPD. The growth in optical density (OD) and carotenoid production (mg/L) of the cultures reached 4.88, 6.76, 5.79, and 0.67, 0.92, and 0.69, respectively. The CD culture served as the basis of an improved specific culture medium containing industrial glucose. Additionally, in this new formulation, vitamins, glutamate, and other compounds were evaluated. Industrial glucose more than doubled carotenoid production; however, the addition of vitamins was not essential for X. dendrorhous cultivation. Moreover, glutamate and Na2HPO4 proved to be highly significant factors (p-value < 0.05), increasing carotenoid biosynthesis from 0.67 to 1.33 mg/L. The specific culture was successfully used in a bioreactor at 2 L and 110 L pilot-scale levels, increasing carotenoid production up to 2 mg/L. It was demonstrated that the CD-specific culture medium is an efficient alternative to conventional culture media to carry out carotenoid production at the laboratory and pilot levels, with promising potential for industrial scaling.
Alejandro Torres-Haro; Anne Gschaedler; Juan Mateos-Díaz; Enrique Herrera-López; Rosa Camacho-Ruíz; Melchor Arellano-Plaza. Improvement of a Specific Culture Medium Based on Industrial Glucose for Carotenoid Production by Xanthophyllomyces dendrorhous. Processes 2021, 9, 429 .
AMA StyleAlejandro Torres-Haro, Anne Gschaedler, Juan Mateos-Díaz, Enrique Herrera-López, Rosa Camacho-Ruíz, Melchor Arellano-Plaza. Improvement of a Specific Culture Medium Based on Industrial Glucose for Carotenoid Production by Xanthophyllomyces dendrorhous. Processes. 2021; 9 (3):429.
Chicago/Turabian StyleAlejandro Torres-Haro; Anne Gschaedler; Juan Mateos-Díaz; Enrique Herrera-López; Rosa Camacho-Ruíz; Melchor Arellano-Plaza. 2021. "Improvement of a Specific Culture Medium Based on Industrial Glucose for Carotenoid Production by Xanthophyllomyces dendrorhous." Processes 9, no. 3: 429.
Antecedentes: La fisiología de la materia prima en los procesos de extracción de fructanos es imprescindible para establecer criterios que garanticen homogeneidad en la calidad del producto, así, la madurez fisiológica plena (MFP) para la extracción de fructanos en las especies de Agave puede reducir la variabilidad de su calidad. Preguntas: ¿La MFP de los agaves y el uso exclusivo de su tallo influye sobre el perfil de carbohidratos y el GP promedio de sus fructanos? Especies de estudio: Agave salmiana Otto ex Salm-Dyck (ASE) y A. tequilana F.A.C. Weber (ATE) Sitio de estudio y periodo de investigación: El estudio fue realizado con cabezas de maguey mezcalero potosino recolectadas en agostaderos ejidales del municipio de Charcas, San Luis Potosí y cabezas de maguey tequilero que se cosecharon en Arandas, Jalisco; durante la primavera de (2019). Métodos: Por medio de HPTLC, HPLC y HP-SEC se caracterizó y estimó la distribución del perfil de carbohidratos y el promedio del GP de los fructanos de tallos de ASE y ATE con MFP y el mismo proceso orgánico de extracción, y de fructanos comerciales de A. tequilana (ATC) y de achicoria (IC). Resultados: Se encontró un GP de 14.46, 16.20, 18.25 y 21.11 para IC, ASE, ATC y ATE, respectivamente. Conclusión: La MFP en las plantas de Agave y el uso exclusivo de sus tallos se confirmó que el GP para ASE es 16 y se presenta por primera vez un GP de 21 para A. tequilana F.A.C. Weber bajo el proceso descrito de extracción.
Evelyn Regalado Rentería; César Iván Godínez-Hernández; Juan Rogelio Aguirre Rivera; Rosa María Camacho Ruíz; Bertha Irene Juárez Flores. Caracterización de fructanos de tallos de Agave salmiana Otto ex Salm Dyck y A. tequilana F.A.C. Weber en madurez fisiológica plena. Botanical Sciences 2021, 99, 388 -397.
AMA StyleEvelyn Regalado Rentería, César Iván Godínez-Hernández, Juan Rogelio Aguirre Rivera, Rosa María Camacho Ruíz, Bertha Irene Juárez Flores. Caracterización de fructanos de tallos de Agave salmiana Otto ex Salm Dyck y A. tequilana F.A.C. Weber en madurez fisiológica plena. Botanical Sciences. 2021; 99 (2):388-397.
Chicago/Turabian StyleEvelyn Regalado Rentería; César Iván Godínez-Hernández; Juan Rogelio Aguirre Rivera; Rosa María Camacho Ruíz; Bertha Irene Juárez Flores. 2021. "Caracterización de fructanos de tallos de Agave salmiana Otto ex Salm Dyck y A. tequilana F.A.C. Weber en madurez fisiológica plena." Botanical Sciences 99, no. 2: 388-397.
Ceramic and polymeric membrane systems were compared at the pilot scale for separating agave fructans into different molecular weight fractions that help to diversify them into more specific industrial applications. The effect of the transmembrane pressure of ultrafiltration performance was evaluated through hydraulic permeability, permeate flux and rejection coefficients, using the same operating conditions such as temperature, feed concentration and the molecular weight cut-off (MWCO) of membranes. The fouling phenomenon and the global yield of the process were evaluated in concentration mode. A size distribution analysis of agave fructans is presented and grouped by molecular weight in different fractions. Great differences were found between both systems, since rejection coefficients of 68.6% and 100% for fructans with degrees of polymerization (DP) > 10, 36.3% and 99.3% for fructooligosaccharides (FOS) and 21.4% and 34.2% for mono-disaccharides were obtained for ceramic and polymeric membrane systems, respectively. Thus, ceramic membranes are better for use in the fractionation process since they reached a purity of 42.2% of FOS with a yield of 40.1% in the permeate and 78.23% for fructans with DP > 10 and a yield of 70% in the retentate. Polymeric membranes make for an efficient fructan purification process, eliminating only mono-disaccharides, and reaching a 97.7% purity (considering both fructan fractions) with a yield of 64.3% in the retentate.
Noe Luiz-Santos; Rogelio Prado-Ramírez; Enrique Arriola-Guevara; Rosa-María Camacho-Ruiz; Lorena Moreno-Vilet. Performance Evaluation of Tight Ultrafiltration Membrane Systems at Pilot Scale for Agave Fructans Fractionation and Purification. Membranes 2020, 10, 261 .
AMA StyleNoe Luiz-Santos, Rogelio Prado-Ramírez, Enrique Arriola-Guevara, Rosa-María Camacho-Ruiz, Lorena Moreno-Vilet. Performance Evaluation of Tight Ultrafiltration Membrane Systems at Pilot Scale for Agave Fructans Fractionation and Purification. Membranes. 2020; 10 (10):261.
Chicago/Turabian StyleNoe Luiz-Santos; Rogelio Prado-Ramírez; Enrique Arriola-Guevara; Rosa-María Camacho-Ruiz; Lorena Moreno-Vilet. 2020. "Performance Evaluation of Tight Ultrafiltration Membrane Systems at Pilot Scale for Agave Fructans Fractionation and Purification." Membranes 10, no. 10: 261.
Alejandra Barbachano‐Torres; Mayra A. López‐Ortega; Mariana Delgado‐García; Yolanda González‐García; Jorge A. Rodríguez; Manuel R. Kirchmayr; Rosa M. Camacho‐Ruíz. Production and Characterization of Surface‐Active Lipopeptides by Haloalkaliphilic Bacteria Salibacterium sp. 4CTb. Journal of Surfactants and Detergents 2019, 23, 67 -78.
AMA StyleAlejandra Barbachano‐Torres, Mayra A. López‐Ortega, Mariana Delgado‐García, Yolanda González‐García, Jorge A. Rodríguez, Manuel R. Kirchmayr, Rosa M. Camacho‐Ruíz. Production and Characterization of Surface‐Active Lipopeptides by Haloalkaliphilic Bacteria Salibacterium sp. 4CTb. Journal of Surfactants and Detergents. 2019; 23 (1):67-78.
Chicago/Turabian StyleAlejandra Barbachano‐Torres; Mayra A. López‐Ortega; Mariana Delgado‐García; Yolanda González‐García; Jorge A. Rodríguez; Manuel R. Kirchmayr; Rosa M. Camacho‐Ruíz. 2019. "Production and Characterization of Surface‐Active Lipopeptides by Haloalkaliphilic Bacteria Salibacterium sp. 4CTb." Journal of Surfactants and Detergents 23, no. 1: 67-78.
A comparative data of two generally accepted mass techniques for estimate the molecular weight distribution of polysaccharides are presented. The data were obtained from agave fructans samples of different molecular weight, which were analyzed authored independently. The two analytical techniques used were Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-ToF) and High Pressure Size Exclusion Chromatography (HP-SEC). The data set here are related to the research paper entitled “Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans” by Moreno-Vilet et al. [1]. This article present the comparative figures as histograms obtained from mathematically processed MALDI-ToF spectra and HPLC-SEC chromatograms. And also, the calculated polymer parameters as number and mass molecular weight, number and mass average degree of polymerization and dispersity index.
Lorena Moreno-Vilet; Stéphane Bostyn; José-Luis Flores-Montaño; Rosa-María Camacho-Ruiz. Comparative data of molecular weight distribution of agave fructans fractions using MALDI-ToF and HPLC-SEC. Data in Brief 2019, 24, 103984 .
AMA StyleLorena Moreno-Vilet, Stéphane Bostyn, José-Luis Flores-Montaño, Rosa-María Camacho-Ruiz. Comparative data of molecular weight distribution of agave fructans fractions using MALDI-ToF and HPLC-SEC. Data in Brief. 2019; 24 ():103984.
Chicago/Turabian StyleLorena Moreno-Vilet; Stéphane Bostyn; José-Luis Flores-Montaño; Rosa-María Camacho-Ruiz. 2019. "Comparative data of molecular weight distribution of agave fructans fractions using MALDI-ToF and HPLC-SEC." Data in Brief 24, no. : 103984.
Agave salmiana represents a high economic and cultural relevance in Mexican society. Cultivated in arid zones of Mexico, it is the agave species primarily used for the production of mezcal, a traditional Mexican alcoholic beverage. In this study, we performed a morphological comparison among 145 accessions of Agave salmiana ssp. crassispina and ssp. salmiana from 14 municipalities in northern Guanajuato. A total of 12 morphological variables of leaf size, spines and teeth were measured. In order to perform this evaluation, agave plants were considered either as cultivated or wild-type. Statistical analyzes were conducted and a dendrogram was constructed with the nearest neighbor method, analysis of variance (ANOVA) and Fisher’s Least Significant Difference (LSD) multiple comparisons. Several groups were established within the dendrogram and the distance among the groups was found to be small. The ANOVA shows significant differences among the subspecies and the populations, whereas the multiple comparisons detected several groups. The statistical analyses showed a morphological variation among the studied plants. However, the whole population under study falls within a range of morphological homogeneity.
José Juvencio Castañeda-Nava; José Manuel Rodríguez-Domínguez; Rosa María Camacho-Ruiz; Juan Gallardo-Valdez; Edgardo Villegas-García; Antonia Gutiérrez-Mora. Morphological comparison among populations of Agave salmiana Otto ex Salm-Dyck (Asparagaceae), a species used for mezcal production in Mexico. Flora 2019, 255, 18 -23.
AMA StyleJosé Juvencio Castañeda-Nava, José Manuel Rodríguez-Domínguez, Rosa María Camacho-Ruiz, Juan Gallardo-Valdez, Edgardo Villegas-García, Antonia Gutiérrez-Mora. Morphological comparison among populations of Agave salmiana Otto ex Salm-Dyck (Asparagaceae), a species used for mezcal production in Mexico. Flora. 2019; 255 ():18-23.
Chicago/Turabian StyleJosé Juvencio Castañeda-Nava; José Manuel Rodríguez-Domínguez; Rosa María Camacho-Ruiz; Juan Gallardo-Valdez; Edgardo Villegas-García; Antonia Gutiérrez-Mora. 2019. "Morphological comparison among populations of Agave salmiana Otto ex Salm-Dyck (Asparagaceae), a species used for mezcal production in Mexico." Flora 255, no. : 18-23.
Biologically active peptides produced from fish wastes are gaining attention because their health benefits. Proteases produced by halophilic microorganisms are considered as a source of active enzymes in high salt systems like fish residues. Hence, the aim of this study was the bioprospection of halophilic microorganisms for the production of proteases to prove their application for peptide production. Halophilic microorganisms were isolated from saline soils of Mexico and Bolivia. An enzymatic screening was carried out for the detection of lipases, esterases, pHB depolymerases, chitinases, and proteases. Most of the strains were able to produce lipases, esterases, and proteases, and larger hydrolysis halos were detected for protease activity. Halobacillus andaensis was selected to be studied for proteolytic activity production; the microorganism was able to grow on gelatin, yeast extract, skim milk, casein, peptone, fish muscle (Cyprinus carpio), and soy flour as protein sources, and among these sources, fish muscle protein was the best inducer of proteolytic activity, achieving a protease production of 571 U/mL. The extracellular protease was active at 50°C, pH 8, and 1.4 M NaCl and was inhibited by phenylmethylsulfonyl fluoride. The proteolytic activity of H. andaensis was used to hydrolyze fish muscle protein for peptide production. The peptides obtained showed a MW of 5.3 kDa and a radical scavenging ability of 10 to 30% on 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and a ferric reducing ability of plasma. The use of noncommercial extracellular protease produced by H. andaensis for biologically active peptide production using fish muscle as the protein source presents a great opportunity for high-value peptide production. How to cite: Delgado-García M, Flores-Gallegos AC, Kirchmayr M, et al. Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein. Electron J Biotechnol 2019;39. https://doi.org/10.1016/j.ejbt.2019.03.001.
Mariana Delgado-García; Adriana C. Flores-Gallegos; Manuel Kirchmayr; Jorge A. Rodríguez; Juan C. Mateos-Díaz; Cristobal N. Aguilar; Marcelo Muller; Rosa M. Camacho-Ruíz. Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein. Electronic Journal of Biotechnology 2019, 39, 52 -60.
AMA StyleMariana Delgado-García, Adriana C. Flores-Gallegos, Manuel Kirchmayr, Jorge A. Rodríguez, Juan C. Mateos-Díaz, Cristobal N. Aguilar, Marcelo Muller, Rosa M. Camacho-Ruíz. Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein. Electronic Journal of Biotechnology. 2019; 39 ():52-60.
Chicago/Turabian StyleMariana Delgado-García; Adriana C. Flores-Gallegos; Manuel Kirchmayr; Jorge A. Rodríguez; Juan C. Mateos-Díaz; Cristobal N. Aguilar; Marcelo Muller; Rosa M. Camacho-Ruíz. 2019. "Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein." Electronic Journal of Biotechnology 39, no. : 52-60.
Lipases and esterases are able to hydrolyze triglycerides, and they are important biocatalysts in food industry. There are many sources of these enzymes. One of the most interesting and unexplored sources of microbial lipases/esterases is the Archaea domain. Most of the archaea produces “extremozymes” that are able to act at harsh conditions such as high temperature, acidic or alkaline pH, high pressure, etc. Particularly, halophilic archaea is a group of microorganisms able to produce lipases and esterases active at a high salt concentration. Nowadays, only a few lipases/esterases from halophilic archaea have been described; however, the reports indicate that halophilic archaeal lipases/esterases are stable at neutral pH, high salt concentration, high temperature, and in hydrophilic solvents. This chapter describes the special features of halophilic archaeal lipases and esterases characterized until now and optimal conditions for their activity and solvent stability. Their substrate preferences were analyzed. Halophilic archaeal lipase/esterase sequences reported in different databases were analyzed. With this analysis we found the evidence of the typical characteristics of lipases/esterases from halophilic archaea: acidic isoelectric point, low molecular weight, and a high content of aspartic and glutamic amino acids. The few halophilic lipases and esterases characterized until now show a preference to hydrolyze vinyl esters and mono- and diglycerides with low activity in triglycerides. Studies on the application of halophilic lipases and esterases in food industry are scarce. However, they have great potential in some processes, like in synthesis of high-value products like structured lipids useful as food additives. The use of lipolytic archaeal enzymes in seafood ripening is an emerging trend. An extensive research is needed in order to develop food applications.
M. Delgado-García; J. A. Rodríguez; Juan Carlos Mateos-Díaz; C. N. Aguilar; R. Rodríguez-Herrera; R. M. Camacho-Ruíz. Halophilic Archaeal Lipases and Esterases: Activity, Stability, and Food Applications. Enzymes in Food Technology 2018, 243 -262.
AMA StyleM. Delgado-García, J. A. Rodríguez, Juan Carlos Mateos-Díaz, C. N. Aguilar, R. Rodríguez-Herrera, R. M. Camacho-Ruíz. Halophilic Archaeal Lipases and Esterases: Activity, Stability, and Food Applications. Enzymes in Food Technology. 2018; ():243-262.
Chicago/Turabian StyleM. Delgado-García; J. A. Rodríguez; Juan Carlos Mateos-Díaz; C. N. Aguilar; R. Rodríguez-Herrera; R. M. Camacho-Ruíz. 2018. "Halophilic Archaeal Lipases and Esterases: Activity, Stability, and Food Applications." Enzymes in Food Technology , no. : 243-262.
Cultivable halophilic microorganisms were isolated and identified from saline and alkaline-sodic soils: Cuatro Cienegas, Sayula and San Marcos lakes. Physicochemical characteristics of soils were determined to understand the relationship between those and the microorganisms isolated. The Cuatro Cienegas soils had a neutral pH, EC of 2.3–8 dS cm−1, classified as moderately saline. Whereas, the soils from Sayula and San Marcos lakes, had an alkaline pH, EC 15 to 65 dS m−1, typical of saline-sodic. We identified 23 cultivable halophilic bacteria using 16s rDNA, being Halobacillus sp., Marinococcus sp., and Alkalibacillus sp. the predominant genus by culture dependent approach. We found a correlation between the soils anion and cation content with the occurrence of different genus of halophilic bacteria in each studied site. Alkalibacillus sp. was predominant in Sayula and San Marcos lakes and was related to the high Na+ content; while Bacillus sp. and Halobacillus sp. were predominant in Cuatro Cienegas, their occurrence was related to a high content of Ca2+, Mg2+, and SO42-.
Mariana Delgado-García; Silvia Maribel Contreras-Ramos; Jorge Alberto Rodríguez; Juan Carlos Mateos-Díaz; Cristóbal Noé Aguilar; Rosa María Camacho-Ruíz. Isolation of halophilic bacteria associated with saline and alkaline-sodic soils by culture dependent approach. Heliyon 2018, 4, e00954 .
AMA StyleMariana Delgado-García, Silvia Maribel Contreras-Ramos, Jorge Alberto Rodríguez, Juan Carlos Mateos-Díaz, Cristóbal Noé Aguilar, Rosa María Camacho-Ruíz. Isolation of halophilic bacteria associated with saline and alkaline-sodic soils by culture dependent approach. Heliyon. 2018; 4 (11):e00954.
Chicago/Turabian StyleMariana Delgado-García; Silvia Maribel Contreras-Ramos; Jorge Alberto Rodríguez; Juan Carlos Mateos-Díaz; Cristóbal Noé Aguilar; Rosa María Camacho-Ruíz. 2018. "Isolation of halophilic bacteria associated with saline and alkaline-sodic soils by culture dependent approach." Heliyon 4, no. 11: e00954.
Carbohydrate esterases are a group of enzymes which release acyl or alkyl groups attached by ester linkage to carbohydrates. The CAZy database, which classifies enzymes that assemble, modify, and break down carbohydrates and glycoconjugates, classifies all carbohydrate esterases into 16 families. This chapter is an overview of the research for nearly 50 years around the main groups of carbohydrate esterases dealing with the degradation of polysaccharides, their main biochemical and molecular traits, as well as its application for the synthesis of high added value esters.
Mariana Armendáriz-Ruiz; Jorge Alberto Rodríguez González; Rosa María Camacho-Ruíz; Juan Carlos Mateos-Díaz. Carbohydrate Esterases: An Overview. Methods in Molecular Biology 2018, 1835, 39 -68.
AMA StyleMariana Armendáriz-Ruiz, Jorge Alberto Rodríguez González, Rosa María Camacho-Ruíz, Juan Carlos Mateos-Díaz. Carbohydrate Esterases: An Overview. Methods in Molecular Biology. 2018; 1835 ():39-68.
Chicago/Turabian StyleMariana Armendáriz-Ruiz; Jorge Alberto Rodríguez González; Rosa María Camacho-Ruíz; Juan Carlos Mateos-Díaz. 2018. "Carbohydrate Esterases: An Overview." Methods in Molecular Biology 1835, no. : 39-68.
Agave fructans are increasingly important in food industry and nutrition sciences as a potential ingredient of functional food, thus practical analysis tools to characterize them are needed. In view of the importance of the molecular weight on the functional properties of agave fructans, this study has the purpose to optimize a method to determine their molecular weight distribution by HPLC-SEC for industrial application. The optimization was carried out using a simplex method. The optimum conditions obtained were at column temperature of 61.7°C using tri-distilled water without salt, adjusted pH of 5.4 and a flow rate of 0.36mL/min. The exclusion range is from 1 to 49 of polymerization degree (180-7966Da). This proposed method represents an accurate and fast alternative to standard methods involving multiple-detection or hydrolysis of fructans. The industrial applications of this technique might be for quality control, study of fractionation processes and determination of purity.
Lorena Moreno-Vilet; Stéphane Bostyn; Jose-Luis Flores-Montaño; Rosa-María Camacho-Ruiz. Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans. Food Chemistry 2017, 237, 833 -840.
AMA StyleLorena Moreno-Vilet, Stéphane Bostyn, Jose-Luis Flores-Montaño, Rosa-María Camacho-Ruiz. Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans. Food Chemistry. 2017; 237 ():833-840.
Chicago/Turabian StyleLorena Moreno-Vilet; Stéphane Bostyn; Jose-Luis Flores-Montaño; Rosa-María Camacho-Ruiz. 2017. "Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans." Food Chemistry 237, no. : 833-840.
Fructans from agave have received specific attention because of their highly branched fructan content. We have previously reported that the degree of polymerization (dp) influences their biological activity. Therefore, the aim of this study was to investigate the effect of unfractionated and fractionated fructans (higher and lower dps) from Agave tequilana in high-fat diet-induced (HFD) obese mice. Fructans with a lower dp (HFD+ScF) decreased weight gain by 30 %, body fat mass by 51 %, hyperglycemia by 25 % and liver steatosis by 40 %. Interestingly, unfractionated fructans (HFD+F) decreased glucose and triglycerides (TG), whereas fractionated fructans with a higher dp (HFD+LcF) decreased TG but not glucose; in contrast, HFD+ScF decreased glucose but not TG. Our findings suggest that both higher and lower dp agave fructans have complementary effects in metabolic disorders related to obesity. These findings may contribute to the development of improved food supplements with a specific ratio combination of fructans with different dps.
A. L. Márquez-Aguirre; R. M. Camacho-Ruíz; Yanet Karina Gutiérrez Mercado; E. Padilla-Camberos; M. González-Ávila; F. J. Gálvez-Gastélum; N. E. Díaz-Martínez; D. Ortuño-Sahagún. Fructans from Agave tequilana with a Lower Degree of Polymerization Prevent Weight Gain, Hyperglycemia and Liver Steatosis in High-Fat Diet-Induced Obese Mice. Plant Foods for Human Nutrition 2016, 71, 416 -421.
AMA StyleA. L. Márquez-Aguirre, R. M. Camacho-Ruíz, Yanet Karina Gutiérrez Mercado, E. Padilla-Camberos, M. González-Ávila, F. J. Gálvez-Gastélum, N. E. Díaz-Martínez, D. Ortuño-Sahagún. Fructans from Agave tequilana with a Lower Degree of Polymerization Prevent Weight Gain, Hyperglycemia and Liver Steatosis in High-Fat Diet-Induced Obese Mice. Plant Foods for Human Nutrition. 2016; 71 (4):416-421.
Chicago/Turabian StyleA. L. Márquez-Aguirre; R. M. Camacho-Ruíz; Yanet Karina Gutiérrez Mercado; E. Padilla-Camberos; M. González-Ávila; F. J. Gálvez-Gastélum; N. E. Díaz-Martínez; D. Ortuño-Sahagún. 2016. "Fructans from Agave tequilana with a Lower Degree of Polymerization Prevent Weight Gain, Hyperglycemia and Liver Steatosis in High-Fat Diet-Induced Obese Mice." Plant Foods for Human Nutrition 71, no. 4: 416-421.
Agave fructans are complex mixtures of branched fructose polymers with different-sized chains containing β(2-1) and β(2-6) linkages. In the last decade, the interest in studying the functional properties of agave fructans has increased. However, only a few studies in vivo and in vitro are available in the literature. These reports include studies of the prebiotic effect in obesity, blood lipids, cholesterol, and toxicological and immunological studies. This chapter includes information about studies on agave fructans from different sources implicated in health and gives special attention to immune aspects as a prospective field of investigation. Comparisons between inulin-type fructans and agave fructans are discussed.
L. Moreno-Vilet; R.M. Camacho-Ruíz; Diana Portales-Pérez. Prebiotic Agave Fructans and Immune Aspects. Probiotics, Prebiotics, and Synbiotics 2016, 165 -179.
AMA StyleL. Moreno-Vilet, R.M. Camacho-Ruíz, Diana Portales-Pérez. Prebiotic Agave Fructans and Immune Aspects. Probiotics, Prebiotics, and Synbiotics. 2016; ():165-179.
Chicago/Turabian StyleL. Moreno-Vilet; R.M. Camacho-Ruíz; Diana Portales-Pérez. 2016. "Prebiotic Agave Fructans and Immune Aspects." Probiotics, Prebiotics, and Synbiotics , no. : 165-179.
R. Melgarejo-Torres; Carlos O. Castillo-Araiza; P. López-Ordaz; N.V. Calleja-Castañeda; J.L. Cano-Velasco; Rosa Maria Camacho Ruiz; Gary Lye; S. Huerta-Ochoa. Evaluation of ionic liquids as dispersed phase during the production of lactones with E. coli in a three phase partitioning bioreactor. Chemical Engineering Journal 2015, 279, 379 -386.
AMA StyleR. Melgarejo-Torres, Carlos O. Castillo-Araiza, P. López-Ordaz, N.V. Calleja-Castañeda, J.L. Cano-Velasco, Rosa Maria Camacho Ruiz, Gary Lye, S. Huerta-Ochoa. Evaluation of ionic liquids as dispersed phase during the production of lactones with E. coli in a three phase partitioning bioreactor. Chemical Engineering Journal. 2015; 279 ():379-386.
Chicago/Turabian StyleR. Melgarejo-Torres; Carlos O. Castillo-Araiza; P. López-Ordaz; N.V. Calleja-Castañeda; J.L. Cano-Velasco; Rosa Maria Camacho Ruiz; Gary Lye; S. Huerta-Ochoa. 2015. "Evaluation of ionic liquids as dispersed phase during the production of lactones with E. coli in a three phase partitioning bioreactor." Chemical Engineering Journal 279, no. : 379-386.
The effect of carbon source, carbon to nitrogen (C/N) ratio, and limitation in nutrients (N, P, K, Ca, Mg, and Fe) on extracellular polymeric substances (EPS) synthesis by the marine bacteriumSaccharophagus degradanswas studied. This strain was able to grow in mineral medium and produce EPS with different efficiency according to the C source used (g EPS/L): glucose or starch (1.5 ± 0.2); galactose, sucrose, or xylose (0.7 ± 0.2); and fructose (0.3 ± 0.1). The C/N ratio (glucose/ammonium) had a significant effect on EPS biosynthesis due to its production rise as the C/N ratio increased from 3 to 100 (0.7 to 2.1 g EPS/L). It was also observed that limitation in nutrients such as N, P, K, Ca, Mg, and Fe also favored EPS biosynthesis. When taking into account both factors (C/N ratio, 100; nutrients limitation, 50%) a positive synergistic effect was noted on EPS production since under these conditions the maximum concentration obtained was 4.12 ± 0.3 g/L after 72 h of culture. The polymer was found to be a polysaccharide of mainly glucose, mannose, and galactose. This is the first report on EPS production byS. degradanswhich is a new feature of this versatile marine bacterium.
Yolanda González-García; Alejandra Heredia; Juan Carlos Meza-Contreras; Froylán M. E. Escalante; Rosa Maria Camacho Ruiz; Jesú Rdova. Biosynthesis of Extracellular Polymeric Substances by the Marine BacteriumSaccharophagus degradansunder Different Nutritional Conditions. International Journal of Polymer Science 2015, 2015, 1 -7.
AMA StyleYolanda González-García, Alejandra Heredia, Juan Carlos Meza-Contreras, Froylán M. E. Escalante, Rosa Maria Camacho Ruiz, Jesú Rdova. Biosynthesis of Extracellular Polymeric Substances by the Marine BacteriumSaccharophagus degradansunder Different Nutritional Conditions. International Journal of Polymer Science. 2015; 2015 ():1-7.
Chicago/Turabian StyleYolanda González-García; Alejandra Heredia; Juan Carlos Meza-Contreras; Froylán M. E. Escalante; Rosa Maria Camacho Ruiz; Jesú Rdova. 2015. "Biosynthesis of Extracellular Polymeric Substances by the Marine BacteriumSaccharophagus degradansunder Different Nutritional Conditions." International Journal of Polymer Science 2015, no. : 1-7.
Mariana Armendáriz-Ruiz; Eduardo Mateos-Diaz; Jorge Alberto Rodríguez-González; Rosa María Camacho-Ruiz; Antonia Gutiérrez-Mora; Georgina Sandoval-Fabian; Santiago Gallegos-Tintoré; Juan Carlos Mateos-Diaz. Carica papaya by-products as new biocatalysts for the synthesis of oleic acid esters. Biocatalysis and Biotransformation 2015, 33, 1 -8.
AMA StyleMariana Armendáriz-Ruiz, Eduardo Mateos-Diaz, Jorge Alberto Rodríguez-González, Rosa María Camacho-Ruiz, Antonia Gutiérrez-Mora, Georgina Sandoval-Fabian, Santiago Gallegos-Tintoré, Juan Carlos Mateos-Diaz. Carica papaya by-products as new biocatalysts for the synthesis of oleic acid esters. Biocatalysis and Biotransformation. 2015; 33 (4):1-8.
Chicago/Turabian StyleMariana Armendáriz-Ruiz; Eduardo Mateos-Diaz; Jorge Alberto Rodríguez-González; Rosa María Camacho-Ruiz; Antonia Gutiérrez-Mora; Georgina Sandoval-Fabian; Santiago Gallegos-Tintoré; Juan Carlos Mateos-Diaz. 2015. "Carica papaya by-products as new biocatalysts for the synthesis of oleic acid esters." Biocatalysis and Biotransformation 33, no. 4: 1-8.
Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05–1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed.
Jorge Pliego; Juan Carlos Mateos; Jorge Rodriguez; Francisco Valero; Mireia Baeza; Ricardo Femat; Rosa Camacho; Georgina Sandoval; Enrique J. Herrera-López. Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate. Sensors 2015, 15, 2798 -2811.
AMA StyleJorge Pliego, Juan Carlos Mateos, Jorge Rodriguez, Francisco Valero, Mireia Baeza, Ricardo Femat, Rosa Camacho, Georgina Sandoval, Enrique J. Herrera-López. Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate. Sensors. 2015; 15 (2):2798-2811.
Chicago/Turabian StyleJorge Pliego; Juan Carlos Mateos; Jorge Rodriguez; Francisco Valero; Mireia Baeza; Ricardo Femat; Rosa Camacho; Georgina Sandoval; Enrique J. Herrera-López. 2015. "Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate." Sensors 15, no. 2: 2798-2811.
Fructans are dietary fibers with beneficial effects on the gastrointestinal physiology and offer a promising approach for the treatment of some metabolic disorders associated with obesity. In vitro and in vivo studies were developed to test the safety of fructans obtained from Agave tequilana Weber var. azul. Additionally, an in vivo experiment using a diet-induced obesity model was performed to compare the effect of agave fructans with different degree of polymerization (DP) profiles: agave fructans with DP > 10 (LcF), agave FOS with DP < 10 (ScF), and agave fructans with and without demineralization (dTF, TF) versus commercial chicory fructans (OraftiSynergy1™) on the body weight change, fat, total cholesterol, triglycerides and count of fecal Lactobacillus spp. and Bifidobacterium spp. Results showed that A. tequilana fructans were not mutagenic and were safe even at a dose of 5 g per kg b.w. Obese mice that received ScF showed a significant decrease in body weight gain, fat tissue and total cholesterol without increasing the count of fecal Bifidobacteria. Whereas, obese mice that received LcF and TF showed decreased triglycerides and an increased count of fecal Bifidobacteria. Interestingly, although obese mice that received dTF did not show changes in body weight gain, fat tissue, total cholesterol or triglycerides, they showed an increase in the count of Bifidobacteria. These results demonstrate that both the degree of polymerization and the demineralization process can influence the biological activity of agave fructans.
Ana Laura Márquez-Aguirre; Rosa Maria Camacho Ruiz; Myriam Arriaga-Alba; Eduardo Padilla-Camberos; Manuel Kirchmayr; José Luis Blasco; Marisela González-Avila. Effects of Agave tequilana fructans with different degree of polymerization profiles on the body weight, blood lipids and count of fecal Lactobacilli/Bifidobacteria in obese mice. Food & Function 2013, 4, 1237 -1244.
AMA StyleAna Laura Márquez-Aguirre, Rosa Maria Camacho Ruiz, Myriam Arriaga-Alba, Eduardo Padilla-Camberos, Manuel Kirchmayr, José Luis Blasco, Marisela González-Avila. Effects of Agave tequilana fructans with different degree of polymerization profiles on the body weight, blood lipids and count of fecal Lactobacilli/Bifidobacteria in obese mice. Food & Function. 2013; 4 (8):1237-1244.
Chicago/Turabian StyleAna Laura Márquez-Aguirre; Rosa Maria Camacho Ruiz; Myriam Arriaga-Alba; Eduardo Padilla-Camberos; Manuel Kirchmayr; José Luis Blasco; Marisela González-Avila. 2013. "Effects of Agave tequilana fructans with different degree of polymerization profiles on the body weight, blood lipids and count of fecal Lactobacilli/Bifidobacteria in obese mice." Food & Function 4, no. 8: 1237-1244.