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The thickness shear mode acoustic wave device is of interest for the sensing of biomarkers for diseases in various biological fluids, but suffers from the issue of non-specific adsorption of compounds other than those of interest to the electrode surface, thus affecting the device’s output. The aim of this present study was to determine the level of non-specific adsorption on gold electrodes from serum samples with added ovarian cancer biomarker lysophosphatidic acid in the presence of a surface anti-fouling layer. The latter was an oligoethylene molecule with thiol group for attachment to the electrode surface. It was found that the anti-fouling layer had a minimal effect on the level of both adsorption of components from serum and the marker. This result stands in sharp contrast to the analogous monolayer employed for anti-fouling reduction on silica.
Brian De La Franier; Michael Thompson. Surface Adsorption of the Cancer Biomarker Lysophosphatidic Acid in Serum Studied by Acoustic Wave Biosensor. Materials 2021, 14, 4158 .
AMA StyleBrian De La Franier, Michael Thompson. Surface Adsorption of the Cancer Biomarker Lysophosphatidic Acid in Serum Studied by Acoustic Wave Biosensor. Materials. 2021; 14 (15):4158.
Chicago/Turabian StyleBrian De La Franier; Michael Thompson. 2021. "Surface Adsorption of the Cancer Biomarker Lysophosphatidic Acid in Serum Studied by Acoustic Wave Biosensor." Materials 14, no. 15: 4158.
We report the microfabrication and characterization of gold microband electrodes on silicon using standard microfabrication methods, i.e., lithography and etching techniques. A two-step electrodeposition process was carried out using the on-chip platinum reference and gold counter electrodes, thus incorporating glucose oxidase onto a platinum-modified, gold microband electrode with an o-phenylenediamine and ß-cyclodextrin mixture. The as-fabricated electrodes were studied using optical microscopy, scanning electron microscopy, and atomic force microscopy. The two-step electrodeposition process was conducted in low sample volumes (50 µL) of both solutions required for biosensor construction. Cyclic voltammetry and electrochemical impedance spectroscopy were utilised for electrochemical characterization at each stage of the deposition process. The enzymatic-based microband biosensor demonstrated a linear response to glucose from 2.5–15 mM, using both linear sweep voltammetry and chronoamperometric measurements in buffer-based solutions. The biosensor performance was examined in 30 µL volumes of fetal bovine serum. Whilst a reduction in the sensor sensitivity was evident within 100% serum samples (compared to buffer media), the sensor demonstrated linear glucose detection with increasing glucose concentrations (5–17 mM).
Julia Madden; Colm Barrett; Fathima Laffir; Michael Thompson; Paul Galvin; Alan O’ Riordan. On-Chip Glucose Detection Based on Glucose Oxidase Immobilized on a Platinum-Modified, Gold Microband Electrode. Biosensors 2021, 11, 249 .
AMA StyleJulia Madden, Colm Barrett, Fathima Laffir, Michael Thompson, Paul Galvin, Alan O’ Riordan. On-Chip Glucose Detection Based on Glucose Oxidase Immobilized on a Platinum-Modified, Gold Microband Electrode. Biosensors. 2021; 11 (8):249.
Chicago/Turabian StyleJulia Madden; Colm Barrett; Fathima Laffir; Michael Thompson; Paul Galvin; Alan O’ Riordan. 2021. "On-Chip Glucose Detection Based on Glucose Oxidase Immobilized on a Platinum-Modified, Gold Microband Electrode." Biosensors 11, no. 8: 249.
The interaction of the spike (S) glycoprotein of SARS-CoV-2 with angiotensin-converting enzyme 2 (ACE2) correlates with increased virus transmissibility and disease severity in humans. Two strategies may be considered for preventive or treatment purposes: the blockage of the ACE2 receptors or the shielding of receptor-binding domains (RBD) in the Sprotein of COVID-19, as well as the S2 cleavage site that is used by the furin enzyme of the host cells in the late phase of virus activation. Herein, the interaction of carbon quantum dots (CQDs) with the Sprotein of SARS-CoV-2 was investigated using molecular docking and molecular dynamics. CQD molecules were optimized by the HF/3-21G level of theory; the probable interactions between the CQDs with Sprotein were studied by blind docking mode, considering the Sprotein as the receptor and CQDs as ligands. Ethanol, folic acid, Favipiravir, two kinds of functionalized triangular hexagonal graphene, and four kinds of functionalized CQDs were studied on a comparative basis. The results show that OH and amine-functionalized CQDs tend to interact with three branches of Sprotein, especially RBD. The fact that they can block the S2 cleavage site leads to their potential use as a therapeutic agent.
Zahra Ramezani; Mohammad Dayer; Siamak Noorizadeh; Michael Thompson. Deactivation of SARS-CoV-2 via Shielding of Spike Glycoprotein Using Carbon Quantum Dots: Bioinformatic Perspective. COVID 2021, 1, 120 -129.
AMA StyleZahra Ramezani, Mohammad Dayer, Siamak Noorizadeh, Michael Thompson. Deactivation of SARS-CoV-2 via Shielding of Spike Glycoprotein Using Carbon Quantum Dots: Bioinformatic Perspective. COVID. 2021; 1 (1):120-129.
Chicago/Turabian StyleZahra Ramezani; Mohammad Dayer; Siamak Noorizadeh; Michael Thompson. 2021. "Deactivation of SARS-CoV-2 via Shielding of Spike Glycoprotein Using Carbon Quantum Dots: Bioinformatic Perspective." COVID 1, no. 1: 120-129.
Implantable devices fabricated from austenitic type 316L stainless steel have been employed significantly in medicine, principally because the material displays excellent mechanical characteristics and corrosion resistance. It is well known, however, that interaction of exposure of such a material to blood can initiate platelet adhesion and blood coagulation, leading to a harmful medical condition. In order to prevent undesirable surface platelet adhesion on biomaterials employed in procedures such as renal dialysis, we developed an ultrathin anti-thrombogenic covalently attached monolayer based on monoethylene glycol silane chemistry. This functions by forming an interstitial hydration layer which displays restricted mobility in the prevention of surface fouling. In the present work, the promising anti-thrombogenic properties of this film are examined with respect to platelet aggregation on 316L austenitic stainless steel exposed to whole human blood. Prior to exposure with blood, all major surface modification steps were examined by X-ray photoelectron spectroscopic analysis and surface free-angle measurement by contact angle goniometry. End-stage anti-thrombogenicity detection after 20 min of blood exposure at 100 s−1, 300 s−1, 600 s−1, 750 s−1, and 900 s−1 shear rates revealed that a significant reduction (>90%) of platelet adhesion and aggregation was achieved for surface-modified steel, compared with untreated material. This result is confirmed by experiments conducted in real time for 60-minute exposure to blood at 100 s−1, 600 s−1, and 900 s−1 shear rates.
Tairan Yang; Brian De La Franier; Michael Thompson. Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel. Materials 2021, 14, 2342 .
AMA StyleTairan Yang, Brian De La Franier, Michael Thompson. Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel. Materials. 2021; 14 (9):2342.
Chicago/Turabian StyleTairan Yang; Brian De La Franier; Michael Thompson. 2021. "Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel." Materials 14, no. 9: 2342.
The determination of protease activity is very important for disease diagnosis, drug development, and quality and safety assurance for dairy products. Therefore, the development of low-cost and sensitive methods for assessing protease activity is crucial. We report two approaches for monitoring protease activity: in a volume and at surface, via colorimetric and acoustic wave-based biosensors operated in the thickness-shear mode (TSM), respectively. The TSM sensor was based on a β-casein substrate immobilized on a piezoelectric quartz crystal transducer. After an enzymatic reaction with trypsin, it cleaved the surface-bound β-casein, which increased the resonant frequency of the crystal. The limit of detection (LOD) was 0.48 ± 0.08 nM. A label-free colorimetric assay for trypsin detection has also been performed using β-casein and 6-mercaptohexanol (MCH) functionalized gold nanoparticles (AuNPs/MCH-β-casein). Due to the trypsin cleavage of β-casein, the gold nanoparticles lost shelter, and MCH increased the attractive force between the modified AuNPs. Consequently, AuNPs aggregated, and the red shift of the absorption spectra was observed. Spectrophotometric assay enabled an LOD of 0.42 ± 0.03 nM. The Michaelis–Menten constant, KM, for reverse enzyme reaction has also been estimated by both methods. This value for the colorimetric assay (0.56 ± 0.10 nM) is lower in comparison with those for the TSM sensor (0.92 ± 0.44 nM). This is likely due to the better access of the trypsin to the β-casein substrate at the surface of AuNPs in comparison with those at the TSM transducer.
Ivan Piovarci; Sopio Melikishvili; Marek Tatarko; Tibor Hianik; Michael Thompson. Detection of Sub-Nanomolar Concentration of Trypsin by Thickness-Shear Mode Acoustic Biosensor and Spectrophotometry. Biosensors 2021, 11, 117 .
AMA StyleIvan Piovarci, Sopio Melikishvili, Marek Tatarko, Tibor Hianik, Michael Thompson. Detection of Sub-Nanomolar Concentration of Trypsin by Thickness-Shear Mode Acoustic Biosensor and Spectrophotometry. Biosensors. 2021; 11 (4):117.
Chicago/Turabian StyleIvan Piovarci; Sopio Melikishvili; Marek Tatarko; Tibor Hianik; Michael Thompson. 2021. "Detection of Sub-Nanomolar Concentration of Trypsin by Thickness-Shear Mode Acoustic Biosensor and Spectrophotometry." Biosensors 11, no. 4: 117.
The ultra-high frequency EMPAS (electromagnetic piezoelectric acoustic sensor) device is composed of an electrode-less quartz disc in which shear oscillation is induced by an AC-powered magnetic coil located 30 μm below the substrate. This configuration allows the instigation of high acoustic harmonics (in the region of 49th–53rd), with the resulting enhanced analytical sensitivity for biosensor purposes compared to the conventional thickness-shear mode device. In this paper, we introduce significant improvements to the operation of the system with respect to sensing applications. This includes a new interface program and the capability to measure the acoustic quality factor not available in the prototype version. The enhanced configuration is subject to testing through biosensor detection of surface adsorption of biological macromolecules, which include β-casein, and a gelsolin-actin complex.
Gábor Mészáros; Sanaz Akbarzadeh; Brian De La Franier; Zsófia Keresztes; Michael Thompson. Advances in Electromagnetic Piezoelectric Acoustic Sensor Technology for Biosensor-Based Detection. Chemosensors 2021, 9, 58 .
AMA StyleGábor Mészáros, Sanaz Akbarzadeh, Brian De La Franier, Zsófia Keresztes, Michael Thompson. Advances in Electromagnetic Piezoelectric Acoustic Sensor Technology for Biosensor-Based Detection. Chemosensors. 2021; 9 (3):58.
Chicago/Turabian StyleGábor Mészáros; Sanaz Akbarzadeh; Brian De La Franier; Zsófia Keresztes; Michael Thompson. 2021. "Advances in Electromagnetic Piezoelectric Acoustic Sensor Technology for Biosensor-Based Detection." Chemosensors 9, no. 3: 58.
The operation of biosensors requires surfaces that are both highly specific towards the target analyte and that are minimally subject to fouling by species present in a biological fluid. In this work, we further examined the thiosulfonate-based linker in order to construct robust and durable self-assembling monolayers (SAMs) onto hydroxylated surfaces such as silica. These SAMs are capable of the chemoselective immobilization of thiol-containing probes (for analytes) under aqueous conditions in a single, straightforward, reliable, and coupling-free manner. The efficacy of the method was assessed through implementation as a biosensing interface for an ultra-high frequency acoustic wave device dedicated to the detection of avidin via attached biotin. Fouling was assessed via introduction of interfering bovine serum albumin (BSA), IgG antibody, or goat serum. Improvements were investigated systematically through the incorporation of an oligoethylene glycol backbone employed together with a self-assembling diluent without a functional distal group. This work demonstrates that the incorporation of a diluent of relatively short length is crucial for the reduction of fouling. Included in this work is a comparison of the surface attachment of the linker to Si3N4 and AlN, both materials used in sensor technology.
Jack Sheng; Brian De La Franier; Michael Thompson. Assembling Surface Linker Chemistry with Minimization of Non-Specific Adsorption on Biosensor Materials. Materials 2021, 14, 472 .
AMA StyleJack Sheng, Brian De La Franier, Michael Thompson. Assembling Surface Linker Chemistry with Minimization of Non-Specific Adsorption on Biosensor Materials. Materials. 2021; 14 (2):472.
Chicago/Turabian StyleJack Sheng; Brian De La Franier; Michael Thompson. 2021. "Assembling Surface Linker Chemistry with Minimization of Non-Specific Adsorption on Biosensor Materials." Materials 14, no. 2: 472.
Indwelling urinary catheters are a common medical device used to relieve urinary retention. Many patients who undergo urinary catheterization develop urinary tract infections (UTIs), which can lead to severe medical complications and high cost of subsequent treatment. Recent years have seen a number of attempts at reducing the rate of UTIs in catheterized patients via catheter surface modifications. In this work, a low cost, robust anti-thrombogenic, and sterilizable anti-fouling layer based on a covalently-bound monoethylene glycol hydroxide (MEG-OH) was attached to polyurethane, a polymeric material commonly used to fabricate catheters. Modified polyurethane tubing was compared to bare tubing after exposure to a wide spectrum of pathogens including Gram-negative bacteria (Pesudomonas aeruginosa, Escherichia coli), Gram-positive bacteria (Staphylococcus aureus) and a fungus (Candida albicans). It has been demonstrated that the MEG-OH monolayer was able to significantly reduce the amount of adhesion of pathogens present on the material surface, with between 85 and 96 % reduction after 24 h of exposure. Additionally, similar reductions in surface fouling were observed following autoclave sterilization, long term storage of samples in air, and longer exposure up to 3 days.
Brian De La Franier; Dalal Asker; Desmond van Den Berg; Benjamin Hatton; Michael Thompson. Reduction of microbial adhesion on polyurethane by a sub-nanometer covalently-attached surface modifier. Colloids and Surfaces B: Biointerfaces 2021, 200, 111579 .
AMA StyleBrian De La Franier, Dalal Asker, Desmond van Den Berg, Benjamin Hatton, Michael Thompson. Reduction of microbial adhesion on polyurethane by a sub-nanometer covalently-attached surface modifier. Colloids and Surfaces B: Biointerfaces. 2021; 200 ():111579.
Chicago/Turabian StyleBrian De La Franier; Dalal Asker; Desmond van Den Berg; Benjamin Hatton; Michael Thompson. 2021. "Reduction of microbial adhesion on polyurethane by a sub-nanometer covalently-attached surface modifier." Colloids and Surfaces B: Biointerfaces 200, no. : 111579.
Epilepsy is represented by a set of neurological disorders that result in recurring seizures and convulsions. Although several types of the condition have been characterized, the underlying cause for these remains largely unknown. A number of molecular biomarkers for epilepsy have been identified including glutamate, γ-aminobutyric acid, and miRNAs. In addition, a special role appears to be played by the potassium cation. Detection of these species is anticipated to assist in both diagnosis and fundamental understanding of the condition. This review details the application of a number of biosensor devices that have been designed specifically for the detection of both molecular biomarkers and the K+ cation in proximity to an animal cortex. These devices offer considerable potential not only for diagnostic goals, but also for study of the cause and spread of the epileptic seizure, especially if such biosensors can detect analytes in a multiplexed, real-time manner.
Michael Thompson; Amanda J. Ackroyd. Biosensor Technology in Diagnostics and Mechanistic Studies of Epilepsy. Proceedings of The 1st International Electronic Conference on Biosensors 2020, 22, 20 .
AMA StyleMichael Thompson, Amanda J. Ackroyd. Biosensor Technology in Diagnostics and Mechanistic Studies of Epilepsy. Proceedings of The 1st International Electronic Conference on Biosensors. 2020; 22 (1):20.
Chicago/Turabian StyleMichael Thompson; Amanda J. Ackroyd. 2020. "Biosensor Technology in Diagnostics and Mechanistic Studies of Epilepsy." Proceedings of The 1st International Electronic Conference on Biosensors 22, no. 1: 20.
An electromagnetic piezoelectric acoustic sensor (EMPAS) was used to study the non-specific adsorption of human red blood cell-derived extracellular vesicle preparations. Vesicle storage history (temperature and duration) highly affected the obtained results: The signal change, namely the frequency decrease of the crystal measured at 20 °C, was negligibly small (−2) when the vesicle solutions had previously been stored at 4 °C, and was in the order of 10 s−2 when the vesicle solutions had been stored at −30 °C. Moreover, the rate of frequency decrease increased exponentially with the storage time at −30 °C. Upon a 4 °C storage period following the −30 °C storage period of the same sample, the measured frequency decrease dropped, suggesting a partial relaxation of the system. The results are explained by the disintegration of the vesicles triggered by the freeze–thaw cycle, likely due to the detachment of proteins from the vesicle surface as was proved by size-exclusion chromatography. Surface modification of the sensor crystal provided the possibility of signal enhancement, as the maximum rate of the frequency change for the same vesicle concentrations was higher on hydrophobic, octadecyl trichlorosilane–modified quartz than on hydrophilic, bare quartz. The EMPAS signal has been associated with the amount of detached proteins, which in turn is proportional to the originating vesicle concentration.
Loránd Románszki; Zoltán Varga; Judith Mihály; Zsófia Keresztes; Michael Thompson. Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins. Biosensors 2020, 10, 173 .
AMA StyleLoránd Románszki, Zoltán Varga, Judith Mihály, Zsófia Keresztes, Michael Thompson. Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins. Biosensors. 2020; 10 (11):173.
Chicago/Turabian StyleLoránd Románszki; Zoltán Varga; Judith Mihály; Zsófia Keresztes; Michael Thompson. 2020. "Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins." Biosensors 10, no. 11: 173.
The determination of protease activity is very important for disease diagnosis, drug development, and quality and safety assurance for dairy products. Therefore, the development of low-cost methods for assessing protease activity is critical. Here, we demonstrate that an acoustic wave-based biosensor operated in the thickness-shear mode (TSM) enables the low-cost detection of protease activity in real time. The TSM sensor was based on a protein substrate (PS) β-casein immobilized on a piezoelectric quartz crystal electrode. The β-casein layer was immobilized onto a gold surface by a carboxylate terminated self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid (MUA). The carboxylic acid terminal was activated by the reaction of a mixture of water- soluble N-(3-Dimethylaminopropyl)-N0-ethylcarbodiimide (EDC) and N–Hydroxysuccinimide (NHS) on the electrode surfaces. We demonstrated that β-casein can form a stable assembly on a piezoelectric quartz crystal electrode. After an enzymatic reaction with trypsin, it cleaved the surface-bound β-casein substrate, which increased the frequency of the crystal in a sigmoidal manner. Trypsin was detected in the range of concentrations from 1 to 50 nM. The limit of detection was 0.2 nM. Initial reaction rates measured at different enzyme concentrations have been used to construct a calibration curve. Considering the results obtained, we believe that the TSM biosensor is a useful tool for protease analysis.
Sopio Melikishvili; Tibor Hianik; Michael Thompson. Detection of Sub-Nanomolar Concentration of Trypsin by Thicken-Shear Mode (TSM) Acoustic Wave Biosensor. Proceedings of The 1st International Electronic Conference on Biosensors 2020, 60, 6 .
AMA StyleSopio Melikishvili, Tibor Hianik, Michael Thompson. Detection of Sub-Nanomolar Concentration of Trypsin by Thicken-Shear Mode (TSM) Acoustic Wave Biosensor. Proceedings of The 1st International Electronic Conference on Biosensors. 2020; 60 (1):6.
Chicago/Turabian StyleSopio Melikishvili; Tibor Hianik; Michael Thompson. 2020. "Detection of Sub-Nanomolar Concentration of Trypsin by Thicken-Shear Mode (TSM) Acoustic Wave Biosensor." Proceedings of The 1st International Electronic Conference on Biosensors 60, no. 1: 6.
Kiril Fedorov; Sonia Sheikh; Alex Romaschin; Michael Thompson. Enhanced Long-term Antithrombogenicity Instigated by Covalently-Attached Surface Modifier on Biomedical Polymers. Recent Progress in Materials 2020, 2, 1 -16.
AMA StyleKiril Fedorov, Sonia Sheikh, Alex Romaschin, Michael Thompson. Enhanced Long-term Antithrombogenicity Instigated by Covalently-Attached Surface Modifier on Biomedical Polymers. Recent Progress in Materials. 2020; 2 (3):1-16.
Chicago/Turabian StyleKiril Fedorov; Sonia Sheikh; Alex Romaschin; Michael Thompson. 2020. "Enhanced Long-term Antithrombogenicity Instigated by Covalently-Attached Surface Modifier on Biomedical Polymers." Recent Progress in Materials 2, no. 3: 1-16.
This article explores recent advances in the development of electrochemical biosensors on microneedle platforms towards on-device sensing of biomarkers present in dermal interstitial fluid. The integration of a biosensor with a microneedle platform opens the possibility for minimally invasive bio-chemical detection or continuous monitoring within the dermal interstitial fluid. An introduction to interstitial fluid is provided placing emphasis on sampling methods that have been employed to extract and/or sample tissue fluid for analysis. We look briefly at microneedle technologies used to extract dermal interstitial fluid for subsequent analysis. Successive content will focus on microneedle technologies which have been integrated with electrochemical biosensors for the quantification of various metabolites, electrolytes and other miscellaneous entities known to be present in the dermal interstitial fluid. The review concludes with some of the key challenges and opportunities faced by this next-generation wearable sensing technology.
Julia Madden; Conor O'Mahony; Michael Thompson; Alan O'Riordan; Paul Galvin. Biosensing in dermal interstitial fluid using microneedle based electrochemical devices. Sensing and Bio-Sensing Research 2020, 29, 100348 .
AMA StyleJulia Madden, Conor O'Mahony, Michael Thompson, Alan O'Riordan, Paul Galvin. Biosensing in dermal interstitial fluid using microneedle based electrochemical devices. Sensing and Bio-Sensing Research. 2020; 29 ():100348.
Chicago/Turabian StyleJulia Madden; Conor O'Mahony; Michael Thompson; Alan O'Riordan; Paul Galvin. 2020. "Biosensing in dermal interstitial fluid using microneedle based electrochemical devices." Sensing and Bio-Sensing Research 29, no. : 100348.
A thickness-shear mode acoustic wave biosensor operated within a flow-through system was used to examine the response of mouse retinal tissue to radiation. Control experiments conducted with respect to exposure of the bare gold electrodes of the device under various conditions of light intensity and bathing solution yielded reversible changes in resonant frequency (Fs) and motional resistance (Rm). The magnitude of transient changes was proportional to light intensity, but independent of solution type. These alterations in acoustic parameters were ascribed to acoustic coupling phenomena at the electrode-to-liquid interface. Pre-differentiated retina from mouse samples deposited on the thickness shear mode (TSM) electrode exposed to a high light intensity condition also exhibited reversible changes in both Fs and Rm, compared to control experiments involving a coating used to attach the tissue to the electrode. In this case, the radiation-instigated reversible responses for both acoustic parameters exhibited a reduction in magnitude. The changes are ascribed to the alteration in viscoelasticity of the retinal matrix on the TSM electrode surface. The precise biophysical mechanism responsible for the changes in Fs and Rm remains a challenge, given the complex make up of retinal tissue.
Alin Cheran; Michael Thompson. Radiation-Activated Pre-Differentiated Retinal Tissue Monitored by Acoustic Wave Biosensor. Sensors 2020, 20, 2628 .
AMA StyleAlin Cheran, Michael Thompson. Radiation-Activated Pre-Differentiated Retinal Tissue Monitored by Acoustic Wave Biosensor. Sensors. 2020; 20 (9):2628.
Chicago/Turabian StyleAlin Cheran; Michael Thompson. 2020. "Radiation-Activated Pre-Differentiated Retinal Tissue Monitored by Acoustic Wave Biosensor." Sensors 20, no. 9: 2628.
Rohan R. Ravindranath; Brian De La Franier; Issaka Yougbare; Jason E. Fish; Michael Thompson. Antibody-Based Capture and Behaviour of Endothelial Cell Lines on Pre-Surface Modified Medical Grade Steel. Recent Progress in Materials 2020, 2, 1 -15.
AMA StyleRohan R. Ravindranath, Brian De La Franier, Issaka Yougbare, Jason E. Fish, Michael Thompson. Antibody-Based Capture and Behaviour of Endothelial Cell Lines on Pre-Surface Modified Medical Grade Steel. Recent Progress in Materials. 2020; 2 (1):1-15.
Chicago/Turabian StyleRohan R. Ravindranath; Brian De La Franier; Issaka Yougbare; Jason E. Fish; Michael Thompson. 2020. "Antibody-Based Capture and Behaviour of Endothelial Cell Lines on Pre-Surface Modified Medical Grade Steel." Recent Progress in Materials 2, no. 1: 1-15.
In this paper, an electrochemical technique is introduced for the determination of streptomycin (STR) in the presence of oxytetracycline (OTC) in milk samples. A novel bifunctional modified screen-printed electrode (SPE) modified with oracet blue, silver nanoparticles, and graphene oxide (OB/SNPs/GO/SPE) was fabricated. The modified electrode plays a catalyzer role for electrooxidation of STR at pH = 7.0 and reveals a facile a separation between the oxidation peaks of STR and OTC. Calculation of kinetic parameters such as the electron transfer coefficient α and the heterogeneous rate constant k´ of STR at the OB/SNPs/GO/SPE as 8.1 ± 0.07 cm s−1 and 0.32 have been obtained based on the theoretical model of Andrieux and Saveant. A differential pulse voltammetric measurement demonstrates two linear dynamic ranges, 0.4 to 240.0 nM and 240.0 to 720.0 nM and a detection limit of 0.17 nM for STR. The sensitivities of the OB/SNPs/GO/SPE towards the oxidation of STR in the absence and presence of OTC were 2.625 × 10−1 and 2.633 × 10−1 µA/µM, respectively.
Sanaz Akbarzadeh; Habibollah Khajesharifi; Michael Thompson. Simultaneous Determination of Streptomycin and Oxytetracycline Using a Oracet-Blue/Silver-Nanoparticle/Graphene-Oxide/Modified Screen-Printed Electrode. Biosensors 2020, 10, 23 .
AMA StyleSanaz Akbarzadeh, Habibollah Khajesharifi, Michael Thompson. Simultaneous Determination of Streptomycin and Oxytetracycline Using a Oracet-Blue/Silver-Nanoparticle/Graphene-Oxide/Modified Screen-Printed Electrode. Biosensors. 2020; 10 (3):23.
Chicago/Turabian StyleSanaz Akbarzadeh; Habibollah Khajesharifi; Michael Thompson. 2020. "Simultaneous Determination of Streptomycin and Oxytetracycline Using a Oracet-Blue/Silver-Nanoparticle/Graphene-Oxide/Modified Screen-Printed Electrode." Biosensors 10, no. 3: 23.
This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties.
Sandro Spagnolo; Brian De La Franier; Tibor Hianik; Michael Thompson. Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology. Biosensors 2020, 10, 20 .
AMA StyleSandro Spagnolo, Brian De La Franier, Tibor Hianik, Michael Thompson. Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology. Biosensors. 2020; 10 (3):20.
Chicago/Turabian StyleSandro Spagnolo; Brian De La Franier; Tibor Hianik; Michael Thompson. 2020. "Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology." Biosensors 10, no. 3: 20.
Lysophosphatidic acid (LPA) is present during the medical condition of ovarian cancer at all stages of the disease, and, therefore possesses considerable potential as a biomarker for screening its presence in female patients. Unfortunately, there is currently no clinically employable assay for this biomarker. In the present work, we introduce a test based on the duel protein system of actin and gelsolin that could allow the quantitative measurement of LPA in serum samples in a biosensing format. In order to evaluate this possibility, actin protein was dye-modified and complexed with gelsolin protein, followed by surface deposition onto silica nanoparticles. This solid-phase system was exposed to serum samples containing various concentrations of LPA and analyzed by fluorescence microscopy. Measurements conducted for the LPA-containing serum samples were higher after exposure to the developed test than samples without LPA. Early results suggest a limit of detection of 5 μM LPA in serum. The eventual goal is to employ the chemistry described here in a biosensor configuration for the large population-scale, rapid screening of women for the potential occurrence of ovarian cancer.
Brian De La Franier; Michael Thompson. Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum. Biosensors 2020, 10, 13 .
AMA StyleBrian De La Franier, Michael Thompson. Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum. Biosensors. 2020; 10 (2):13.
Chicago/Turabian StyleBrian De La Franier; Michael Thompson. 2020. "Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum." Biosensors 10, no. 2: 13.
Malignant ovarian Cancer (OC) is the deadliest gynaecological cancer and the sixth most common cause of cancer-related death.
Thompson M. Early Stage Screening for Ovarian Cancer: The Case for Biosensor Technology. Journal of Breast Cancer Research and Advancements 2020, 2, 1 .
AMA StyleThompson M. Early Stage Screening for Ovarian Cancer: The Case for Biosensor Technology. Journal of Breast Cancer Research and Advancements. 2020; 2 (1):1.
Chicago/Turabian StyleThompson M. 2020. "Early Stage Screening for Ovarian Cancer: The Case for Biosensor Technology." Journal of Breast Cancer Research and Advancements 2, no. 1: 1.
Development of a biomaterial that is resistant to the adhesion and consequential proliferation of bacteria, represents a significant challenge in terms of application of such materials in various aspects of health care. Over recent years a large number of synthetic methods have appeared with the overall goal of the prevention of bacterial adhesion to surfaces. In contrast to these artificial techniques, living organisms over millions of years have developed different systems to prevent the colonization of microorganisms. Recently, these natural approaches, which are based on surface nanotopography, have been mimicked to fabricate a modern antibacterial surface. In this vein, use of nanoparticle (NP) technology has been explored in order to create a suitable antibacterial surface. However, few studies have focused on the toxicity of these techniques and the ecotoxicity of NP materials on mammalian and bacterial cells simultaneously. Researchers have observed that the majority of previous studies have demonstrated some of the extents of the harmful impacts on mammalian cells. Here, we provide a critical review of the NP approach to antibacterial surface treatment, and also summarize the studies of toxic effects caused by metal NPs on bacteria and mammalian cells.
Sina Kheiri; Xinyu Liu; Michael Thompson. Nanoparticles at biointerfaces: Antibacterial activity and nanotoxicology. Colloids and Surfaces B: Biointerfaces 2019, 184, 110550 .
AMA StyleSina Kheiri, Xinyu Liu, Michael Thompson. Nanoparticles at biointerfaces: Antibacterial activity and nanotoxicology. Colloids and Surfaces B: Biointerfaces. 2019; 184 ():110550.
Chicago/Turabian StyleSina Kheiri; Xinyu Liu; Michael Thompson. 2019. "Nanoparticles at biointerfaces: Antibacterial activity and nanotoxicology." Colloids and Surfaces B: Biointerfaces 184, no. : 110550.