This page has only limited features, please log in for full access.

Prof. Dr. Antonio Evidente
Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy

Basic Info


Research Keywords & Expertise

0 Biological Control
0 Ecology
0 Organic Chemistry
0 Spectroscopy
0 bioactive natural substances

Fingerprints

Spectroscopy
Biological Control
Ecology

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 31 August 2021 in Biomolecules
Reads 0
Downloads 0

Crinum biflorum Rottb. (syn. Crinum distichum) is an Amaryllidaceae plant used in African traditional medicine but very few studies have been performed on this species from a chemical and applicative point of view. Bulbs of C. biflorum, collected in Senegal, were extracted with ethanol by Soxhlet and the corresponding organic extract was purified using chromatographic methods. The pure compounds were chemically characterized by spectroscopic techniques (1D and 2D 1H and 13C NMR, HR MS and ECD) and X-ray analysis. Four homoisoflavonoids (1–4) and one alkylamide (5) were isolated and characterized as 5,6,7-trimethoxy-3-(4-hydroxybenzyl)chroman-4-one (1), as 3-hydroxy-5,6,7-trimethoxy-3-(4-hydroxybenzyl)chroman-4-one (2), as 3-hydroxy-5,6,7-trimethoxy-3-(4-methoxybenzyl)chroman-4-one (3) and as 5,6,7-trimethoxy-3-(4-methoxybenzyl)chroman-4-one (4), and the alkylamide as (E)-N-(4-hydroxyphenethyl)-3-(4-hydroxyphenyl)acrylamide (5), commonly named N-p-coumaroyltyramine. The relative configuration of compound 1 was verified thanks to the X-ray analysis which also allowed us to confirm its racemic nature. The absolute configurations of compounds 2 and 3 were assigned by comparing their ECD spectra with those previously reported for urgineanins A and B. Flavanoids 1, 3 and 4 showed promising anticancer properties being cytotoxic at low micromolar concentrations towards HeLa and A431 human cancer cell lines. The N-p-coumaroyltyramine (5) was selectively toxic to A431 and HeLa cancer cells while it protected immortalized HaCaT cells against oxidative stress induced by hydrogen peroxide. Compounds 1–4 also inhibited acetylcholinesterase activity with compound 3 being the most potent. The anti-amylase and the strong anti-glucosidase activity of compound 5 were confirmed. Our results show that C. biflorum produces compounds of therapeutic interest with anti-diabetic, anti-tumoral and anti-acetylcholinesterase properties.

ACS Style

Marco Masi; Manoj Koirala; Antonella Delicato; Roberta Di Lecce; Natacha Merindol; Seydou Ka; Matar Seck; Angela Tuzi; Isabel Desgagne-Penix; Viola Calabrò; Antonio Evidente. Isolation and Biological Characterization of Homoisoflavanoids and the Alkylamide N-p-Coumaroyltyramine from Crinum biflorum Rottb., an Amaryllidaceae Species Collected in Senegal. Biomolecules 2021, 11, 1298 .

AMA Style

Marco Masi, Manoj Koirala, Antonella Delicato, Roberta Di Lecce, Natacha Merindol, Seydou Ka, Matar Seck, Angela Tuzi, Isabel Desgagne-Penix, Viola Calabrò, Antonio Evidente. Isolation and Biological Characterization of Homoisoflavanoids and the Alkylamide N-p-Coumaroyltyramine from Crinum biflorum Rottb., an Amaryllidaceae Species Collected in Senegal. Biomolecules. 2021; 11 (9):1298.

Chicago/Turabian Style

Marco Masi; Manoj Koirala; Antonella Delicato; Roberta Di Lecce; Natacha Merindol; Seydou Ka; Matar Seck; Angela Tuzi; Isabel Desgagne-Penix; Viola Calabrò; Antonio Evidente. 2021. "Isolation and Biological Characterization of Homoisoflavanoids and the Alkylamide N-p-Coumaroyltyramine from Crinum biflorum Rottb., an Amaryllidaceae Species Collected in Senegal." Biomolecules 11, no. 9: 1298.

Journal article
Published: 21 August 2021 in Phytochemistry
Reads 0
Downloads 0

Three previously undescribed metabolites named argyrotoxins A-C, were isolated, together with the well known porritoxinol, its closely related phthalide, a phthalide derivative, zinniol, alternariol and its 4-methyl ether from Alternaria argyroxiphii E.G. Simmons & Aragaki, the causal agent of leaf spot on African mahogany trees, Khaya senegalensis A. Juss. (Meliaceae). The known compounds were identified comparing their physical and spectroscopic properties to those previously reported in literature. Argyrotoxins A-C were characterized essentially by NMR (1H, 13C, COSY, HSQC, HMBC and NOESY NMR spectra) and HRESIMS spectra as 4-(7-methoxy-6-methyl-3-oxo-1,3-dihydro-isobenzofuran-5-yloxy)-2-methyl-butyric acid, 5-but-2-enyloxy-N-(2-hydroxyethyl)-2-hydroxymethyl-3-methoxy-4-methyl-benzamide and 1-(5-(hydroxymethyl)-3-methoxy-4-(methoxymethyl)-2-methylphenoxy)-3-methylbutane-2,3-diol, respectively. The absolute configuration of argyrotoxin A was determined through electronic circular dichroism, by applying the biphenyl chiroptical probe approach. The phytoxicity of all metabolites isolated was evaluated by leaf puncture assay at concentration of 1 mg/mL. Zinniol proved to be the most active compound causing necrotic lesions on young leaves of Hedera elix L., Phaseolus vulgaris L. and Quercus ilex L. Argirotoxins A and B were found active, to a minor extent, on Phaseolus vulgaris L. leaves, while porritoxinol exhibited activity on holm oak leaves. The other secondary metabolites herein reported for A. argyroxiphii were inactive.

ACS Style

Marco Masi; Roberta Di Lecce; Lucia Maddau; Giulia Marsico; Stefano Superchi; Antonio Evidente. Argyrotoxins A-C, a trisubstituted dihydroisobenzofuranone, a tetrasubstituted 2-hydroxyethylbenzamide and a tetrasubstitutedphenyl trisubstitutedbutyl ether produced by Alternaria argyroxiphii, the causal agent of leaf spot on African mahogany trees (Khaya senegalensis). Phytochemistry 2021, 191, 112921 .

AMA Style

Marco Masi, Roberta Di Lecce, Lucia Maddau, Giulia Marsico, Stefano Superchi, Antonio Evidente. Argyrotoxins A-C, a trisubstituted dihydroisobenzofuranone, a tetrasubstituted 2-hydroxyethylbenzamide and a tetrasubstitutedphenyl trisubstitutedbutyl ether produced by Alternaria argyroxiphii, the causal agent of leaf spot on African mahogany trees (Khaya senegalensis). Phytochemistry. 2021; 191 ():112921.

Chicago/Turabian Style

Marco Masi; Roberta Di Lecce; Lucia Maddau; Giulia Marsico; Stefano Superchi; Antonio Evidente. 2021. "Argyrotoxins A-C, a trisubstituted dihydroisobenzofuranone, a tetrasubstituted 2-hydroxyethylbenzamide and a tetrasubstitutedphenyl trisubstitutedbutyl ether produced by Alternaria argyroxiphii, the causal agent of leaf spot on African mahogany trees (Khaya senegalensis)." Phytochemistry 191, no. : 112921.

Minireview
Published: 09 July 2021 in ChemBioChem
Reads 0
Downloads 0

Sphaeropsidin A (SphA) is a tetracyclic pimarane diterpene, first isolated as the main phytotoxin produced by Diplodia cupressi the causal agent of a severe canker disease of Italian cypress (Cupressus sempervirens L.). It was also produced, together with several analogues, by different pathogenic Diplodia species and other fungi and showed a broad array of biological activities suggesting its promising application in agriculture and medicine. The anticancer activity of SphA is very potent and cell specific. Recent studies have revealed its unique mode of action. This minireview reports the structures of SphA and its family of natural analogues, their biosynthetic origins, their fungal sources, and biological activities. The preparation of various SphA derivatives is also described as well as the results of structure-activity relationship (SAR) studies and on their potential practical applications.

ACS Style

Marco Masi; Antonio Evidente. Sphaeropsidin A, a pimarane diterpene with interesting biological activities and promising practical applications. ChemBioChem 2021, 1 .

AMA Style

Marco Masi, Antonio Evidente. Sphaeropsidin A, a pimarane diterpene with interesting biological activities and promising practical applications. ChemBioChem. 2021; ():1.

Chicago/Turabian Style

Marco Masi; Antonio Evidente. 2021. "Sphaeropsidin A, a pimarane diterpene with interesting biological activities and promising practical applications." ChemBioChem , no. : 1.

Journal article
Published: 06 July 2021 in Antibiotics
Reads 0
Downloads 0

Cotula cinerea, belonging to the tribe Anthemideae, is a plant widespread in the Southern hemisphere. It is frequently used in folk medicine in North African countries for several of its medical properties, shown by its extracts and essential oils. The dichloromethane extract obtained from its aerial parts demonstrated antibiotic activity against Enterococcus faecalis and was fractionated by bioguided purification procedures affording five main sesquiterpene lactones. They were identified by spectroscopic methods (NMR and ESIMS data) as guaiantrienolides, i.e., 6-acetoxy-1β-,6-acetoxy-1α-, and 6-acetoxy-10-β-hydroxyguaiantrienolide (1–3), and germacrenolides, i.e., haagenolide and 1,10-epoxyhaagenolide (4 and 5). The absolute configuration was assigned by applying the advanced Mosher’s method to haagenolide and by X-ray diffraction analysis to 1,10-epoxyhaagenolide. The specific antibiotic and antibiofilm activities were tested toward the clinical isolates of Enterococcus faecalis. The results showed that compounds 3–5 have antibacterial activity against all the strains of E. faecalis, while compound 2 exhibited activity only toward some strains. Compound 1 did not show this activity but had only antibiofilm properties. Thus, these metabolites have potential as new antibiotics and antibiofilm against drug resistant opportunistic pathogens.

ACS Style

Alessio Cimmino; Emanuela Roscetto; Marco Masi; Angela Tuzi; Imene Radjai; Chakali Gahdab; Rossella Paolillo; Amedeo Guarino; Maria Catania; Antonio Evidente. Sesquiterpene Lactones from Cotula cinerea with Antibiotic Activity against Clinical Isolates of Enterococcus faecalis. Antibiotics 2021, 10, 819 .

AMA Style

Alessio Cimmino, Emanuela Roscetto, Marco Masi, Angela Tuzi, Imene Radjai, Chakali Gahdab, Rossella Paolillo, Amedeo Guarino, Maria Catania, Antonio Evidente. Sesquiterpene Lactones from Cotula cinerea with Antibiotic Activity against Clinical Isolates of Enterococcus faecalis. Antibiotics. 2021; 10 (7):819.

Chicago/Turabian Style

Alessio Cimmino; Emanuela Roscetto; Marco Masi; Angela Tuzi; Imene Radjai; Chakali Gahdab; Rossella Paolillo; Amedeo Guarino; Maria Catania; Antonio Evidente. 2021. "Sesquiterpene Lactones from Cotula cinerea with Antibiotic Activity against Clinical Isolates of Enterococcus faecalis." Antibiotics 10, no. 7: 819.

Journal article
Published: 31 May 2021 in International Journal of Biological Macromolecules
Reads 0
Downloads 0

An O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide (LPS) of Pseudomonas donghuensis SVBP6, a bacterium with a broad-spectrum antifungal activity in vitro, particularly that against Macrophomina phaseolina. This latter is one of the most virulent and dangerous pathogens of plants, including soybean which is an economically important crop in Argentina today. The OPS was studied by sugar analysis and spectroscopy (1D and 2D 1H and 13C NMR) showing the following trisaccharide repeating unit: →6)-ɑ-D-ManpNAc-(1 → 3)-β-l-Rhap-(1 → 4)-β-D-Glcp-(1→. The crude LPS, the purified LPS and the O-chain were assayed for their antifungal activity against M. phaseolina at 25, 50, 100, and 200 μg plug−1. The results showed that the crude LPS best inhibition was at 200 μg plug−1, able to inhibit the fungus growth by about 45%, while purified LPS and the corresponding OPS, in the same condition, reduced fungus growth by 65%, and 75%, respectively. Furthermore, the purified LPS and OPS significantly reduced the growth of M. phaseolina already at 100 μg plug−1 compared to the crude LPS. The structure of the O-chain is unique among the bacterial LPS and this is the first time that both the antifungal activity of a bacterial LPS and its corresponding O-chain were described.

ACS Style

Evelina L. Zdorovenko; Andrey S. Dmitrenok; Marco Masi; Stefany Castaldi; Federico M. Muzio; Rachele Isticato; Claudio Valverde; Yuriy A. Knirel; Antonio Evidente. Structural studies on the O-specific polysaccharide of the lipopolysaccharide from Pseudomonas donghuensis strain SVBP6, with antifungal activity against the phytopathogenic fungus Macrophomina phaseolina. International Journal of Biological Macromolecules 2021, 182, 2019 -2023.

AMA Style

Evelina L. Zdorovenko, Andrey S. Dmitrenok, Marco Masi, Stefany Castaldi, Federico M. Muzio, Rachele Isticato, Claudio Valverde, Yuriy A. Knirel, Antonio Evidente. Structural studies on the O-specific polysaccharide of the lipopolysaccharide from Pseudomonas donghuensis strain SVBP6, with antifungal activity against the phytopathogenic fungus Macrophomina phaseolina. International Journal of Biological Macromolecules. 2021; 182 ():2019-2023.

Chicago/Turabian Style

Evelina L. Zdorovenko; Andrey S. Dmitrenok; Marco Masi; Stefany Castaldi; Federico M. Muzio; Rachele Isticato; Claudio Valverde; Yuriy A. Knirel; Antonio Evidente. 2021. "Structural studies on the O-specific polysaccharide of the lipopolysaccharide from Pseudomonas donghuensis strain SVBP6, with antifungal activity against the phytopathogenic fungus Macrophomina phaseolina." International Journal of Biological Macromolecules 182, no. : 2019-2023.

Journal article
Published: 14 May 2021 in Phytopathologia Mediterranea
Reads 0
Downloads 0

A new cleistanthane nor-diterpenoid, named olicleistanone (1), was isolated as a racemate from the culture filtrates of Diplodia olivarum, an emerging pathogen involved in the aetiology of branch canker and dieback of several plant species typical of the Mediterranean maquis in Sardinia, Italy. When the fungus was grown in vitro on Czapek medium, olicleistanone was isolated together with some already known phytotoxic diterpenoids identified as sphaeropsidins A, C, and G, and diplopimarane (2-5). Olicleistanone was characterized as 4-ethoxy-6a-methoxy-3,8,8-trimethyl-4,5,8,9,10,11-hexahydrodibenzo[de,g]chromen-7(6aH)-one. When D. olivarum was grown on mineral salt medium it produced (-)-mellein (6), sphaeropsidin A and small amounts of sphaeropsidin G and diplopimarane. Olicleistanone (1) exhibited strong activity against the insect Artemia salina L. (100% larval mortality) at 100 μg mL-1 but did not exhibit phytotoxic, antifungal or antioomycete activity. Among the metabolites isolated (1-6), sphaeropsidin A (2) was active in all bioassays performed exhibiting strong phytotoxicity on leaves of Phaseolus vulgaris L., Juglans regia L. and Quercus suber L. at 1 mg mL-1. Sphaeropsidin A (2) also completely inhibited mycelium growth of Athelia rolfsii, Diplodia corticola, Phytophthora cambivora and P. lacustris at 200 μg per plug, and was active in the Artemia salina assay. Also in this assay, diplopimarane (5) and sphaeropsidin G (4) were active (100% larval mortality). Diplopimarane also showed antifungal and antioomycete activities. Athelia rolfsii was the most sensitive species to diplopimarane. Sphaeropsidin C (3) and (-)-mellein (6) were inactive in all bioassays. These results expand knowledge on the metabolic profile of Botryosphaeriaceae, and embody the first characterization of the main secondary metabolites secreted by D. olivarum.

ACS Style

Roberta DI Lecce; Marco Masi; Benedetto Teodoro Linaldeddu; Gennaro Pescitelli; Lucia Maddau; Antonio Evidente. Bioactive secondary metabolites produced by the emerging pathogen Diplodia olivarum. Phytopathologia Mediterranea 2021, 60, 129 -138.

AMA Style

Roberta DI Lecce, Marco Masi, Benedetto Teodoro Linaldeddu, Gennaro Pescitelli, Lucia Maddau, Antonio Evidente. Bioactive secondary metabolites produced by the emerging pathogen Diplodia olivarum. Phytopathologia Mediterranea. 2021; 60 (1):129-138.

Chicago/Turabian Style

Roberta DI Lecce; Marco Masi; Benedetto Teodoro Linaldeddu; Gennaro Pescitelli; Lucia Maddau; Antonio Evidente. 2021. "Bioactive secondary metabolites produced by the emerging pathogen Diplodia olivarum." Phytopathologia Mediterranea 60, no. 1: 129-138.

Journal article
Published: 19 April 2021 in Plants
Reads 0
Downloads 0

Grapevine trunk diseases (GTDs) are considered a serious problem to viticulture worldwide. Several GTD fungal pathogens produce phytotoxic metabolites (PMs) that were hypothesized to migrate to the foliage where they cause distinct symptoms. The role of PMs in the expression of Botryosphaeria dieback (BD) symptoms in naturally infected and artificially inoculated wood using molecular and analytical chemistry techniques was investigated. Wood samples from field vines naturally infected with BD and one-year-old vines inoculated with Diplodia seriata, Spencermartinsia viticola and Dothiorella vidmadera were analysed by cultural isolations, quantitative PCR (qPCR) and targeted LC-MS/MS to detect three PMs: (R)-mellein, protocatechuic acid and spencertoxin. (R)-mellein was detected in symptomatic naturally infected wood and vines artificially inoculated with D. seriata but was absent in all non-symptomatic wood. The amount of (R)-mellein detected was correlated with the amount of pathogen DNA detected by qPCR. Protocatechuic acid and spencertoxin were absent in all inoculated wood samples. (R)-mellein may be produced by the pathogen during infection to break down the wood, however it was not translocated into other parts of the vine. The foliar symptoms previously reported in vineyards may be due to a combination of PMs produced and climatic and physiological factors that require further investigation.

ACS Style

Pierluigi Reveglia; Regina Billones-Baaijens; Jennifer Millera Niem; Marco Masi; Alessio Cimmino; Antonio Evidente; Sandra Savocchia. Production of Phytotoxic Metabolites by Botryosphaeriaceae in Naturally Infected and Artificially Inoculated Grapevines. Plants 2021, 10, 802 .

AMA Style

Pierluigi Reveglia, Regina Billones-Baaijens, Jennifer Millera Niem, Marco Masi, Alessio Cimmino, Antonio Evidente, Sandra Savocchia. Production of Phytotoxic Metabolites by Botryosphaeriaceae in Naturally Infected and Artificially Inoculated Grapevines. Plants. 2021; 10 (4):802.

Chicago/Turabian Style

Pierluigi Reveglia; Regina Billones-Baaijens; Jennifer Millera Niem; Marco Masi; Alessio Cimmino; Antonio Evidente; Sandra Savocchia. 2021. "Production of Phytotoxic Metabolites by Botryosphaeriaceae in Naturally Infected and Artificially Inoculated Grapevines." Plants 10, no. 4: 802.

Journal article
Published: 18 April 2021 in Toxins
Reads 0
Downloads 0

Aedes albopictus (Skuse) is a widespread mosquito, a vector of important human arboviruses, including Chikungunya, Dengue and Zika. It is an extremely difficult species to control even for the onset of resistances to chemicals insecticides, therefore ecofriendly products are urgently needed. In this study, the activity of Amaryllidaceae alkaloids and some of their semisynthetic derivatives, of 2-methoxy-1,4-naphthoquinone and two analogues, of cyclopaldic acid and epi-epoformin on the survival and development of Ae. albopictus larvae was evaluated. First-instar larval exposure for 24 and 48 h to cyclopaldic acid, resulted in mortality mean per-centage of 82.4 and 96.9 respectively; 1,2-O,O-diacetyllycorine 48h post-treatment caused 84.7% mortality. Larval and pupal duration were proved to decrease significantly when larvae were exposed to cyclopaldic acid, 1,2-O,O-diacetyllycorine and N-methyllycorine iodide. The mean number of third-instar larvae surviving to 2-methyl-1,4-naphthoquinone, 2-hydroxy-1,4-naphthoquinone and 2-methoxy-1,4-naphthoquinone was significantly lower than the number of correspondent control larvae over the time. This study indicated that 1,2-O,O’-diacetyllycorine, N-methyllycorine iodide, cyclopaldic acid and 1,4-naphthoquinone structural derivatives have good potential for developing bioinsecticides for mosquito control programs. The obtained results are of general interest due to the global importance of the seri-ous human diseases such a vector is able to spread.

ACS Style

Sonia Ganassi; Marco Masi; Pasqualina Grazioso; Antonio Evidente; Antonio De Cristofaro. Activity of Some Plant and Fungal Metabolites towards Aedes albopictus (Diptera, Culicidae). Toxins 2021, 13, 285 .

AMA Style

Sonia Ganassi, Marco Masi, Pasqualina Grazioso, Antonio Evidente, Antonio De Cristofaro. Activity of Some Plant and Fungal Metabolites towards Aedes albopictus (Diptera, Culicidae). Toxins. 2021; 13 (4):285.

Chicago/Turabian Style

Sonia Ganassi; Marco Masi; Pasqualina Grazioso; Antonio Evidente; Antonio De Cristofaro. 2021. "Activity of Some Plant and Fungal Metabolites towards Aedes albopictus (Diptera, Culicidae)." Toxins 13, no. 4: 285.

Journal article
Published: 11 April 2021 in Plants
Reads 0
Downloads 0

The holoparasitic broomrape weeds (Orobanche and Phelipanche species) cause severe yield losses throughout North Africa, the Middle East, and Southern and Eastern Europe. These parasitic weeds form an haustorium at the tip of their radicles to infect the crop upon detection of the host-derived haustorium-inducing factors. Until now, the haustorial induction in the broomrapes remains less studied than in other parasitic plant species. Known haustorium-inducing factors active in hemiparasites, such as Striga and Triphysaria species, were reported to be inefficient for the induction of haustoria in broomrape radicles. In this work, the haustorium-inducing activity of p-benzoquinone and 2,6-dimethoxy-p-benzoquinone (BQ and DMBQ) on radicles of three different broomrapes, namely Orobanche cumana, Orobanche minor and Phelipanche ramosa, is reported. Additional allelopathic effects of benzoquinones on radicle growth and radicle necrosis were studied. The results of this work suggest that benzoquinones play a role in the induction of haustorium in broomrapes. Although dependent on the broomrape species assayed and the concentration of quinones used in the test, the activity of BQ appeared to be stronger than that of DMBQ. The redox property represented by p-benzoquinone, which operates in several physiological processes of plants, insects and animals, is invoked to explain this different activity. This work confirms the usefulness of benzoquinones as haustorium-inducing factors for holoparasitic plant research. The findings of this work could facilitate future studies in the infection process, such as host-plant recognition and haustorial formation.

ACS Style

Mónica Fernández-Aparicio; Marco Masi; Alessio Cimmino; Antonio Evidente. Effects of Benzoquinones on Radicles of Orobanche and Phelipanche Species. Plants 2021, 10, 746 .

AMA Style

Mónica Fernández-Aparicio, Marco Masi, Alessio Cimmino, Antonio Evidente. Effects of Benzoquinones on Radicles of Orobanche and Phelipanche Species. Plants. 2021; 10 (4):746.

Chicago/Turabian Style

Mónica Fernández-Aparicio; Marco Masi; Alessio Cimmino; Antonio Evidente. 2021. "Effects of Benzoquinones on Radicles of Orobanche and Phelipanche Species." Plants 10, no. 4: 746.

Journal article
Published: 24 March 2021 in International Journal of Molecular Sciences
Reads 0
Downloads 0

In recent decades, intensive crop management has involved excessive use of pesticides or fertilizers, compromising environmental integrity and public health. Accordingly, there has been worldwide pressure to find an eco-friendly and safe strategy to ensure agricultural productivity. Among alternative approaches, Plant Growth-Promoting (PGP) rhizobacteria are receiving increasing attention as suitable biocontrol agents against agricultural pests. In the present study, 22 spore-forming bacteria were selected among a salt-pan rhizobacteria collection for their PGP traits and their antagonistic activity against the plant pathogen fungus Macrophomina phaseolina. Based on the higher antifungal activity, strain RHFS10, identified as Bacillus vallismortis, was further examined and cell-free supernatant assays, column purification, and tandem mass spectrometry were employed to purify and preliminarily identify the antifungal metabolites. Interestingly, the minimum inhibitory concentration assessed for the fractions active against M. phaseolina was 10 times lower and more stable than the one estimated for the commercial fungicide pentachloronitrobenzene. These results suggest the use of B. vallismortis strain RHFS10 as a potential plant growth-promoting rhizobacteria as an alternative to chemical pesticides to efficiently control the phytopathogenic fungus M. phaseolina.

ACS Style

Stefany Castaldi; Claudia Petrillo; Giuliana Donadio; Fabrizio Piaz; Alessio Cimmino; Marco Masi; Antonio Evidente; Rachele Isticato. Plant Growth Promotion Function of Bacillus sp. Strains Isolated from Salt-Pan Rhizosphere and Their Biocontrol Potential against Macrophomina phaseolina. International Journal of Molecular Sciences 2021, 22, 3324 .

AMA Style

Stefany Castaldi, Claudia Petrillo, Giuliana Donadio, Fabrizio Piaz, Alessio Cimmino, Marco Masi, Antonio Evidente, Rachele Isticato. Plant Growth Promotion Function of Bacillus sp. Strains Isolated from Salt-Pan Rhizosphere and Their Biocontrol Potential against Macrophomina phaseolina. International Journal of Molecular Sciences. 2021; 22 (7):3324.

Chicago/Turabian Style

Stefany Castaldi; Claudia Petrillo; Giuliana Donadio; Fabrizio Piaz; Alessio Cimmino; Marco Masi; Antonio Evidente; Rachele Isticato. 2021. "Plant Growth Promotion Function of Bacillus sp. Strains Isolated from Salt-Pan Rhizosphere and Their Biocontrol Potential against Macrophomina phaseolina." International Journal of Molecular Sciences 22, no. 7: 3324.

Journal article
Published: 13 March 2021 in Plants
Reads 0
Downloads 0

Allelopathic potential of buckwheat roots on the radicle growth of the broomrape weed species Orobanche cumana and Phelipanche ramosa was studied. Buckwheat root exudates induced a significant growth inhibition in P. ramosa radicles but radicles of O. cumana were not affected. Among the metabolites present in the root organic extract we identified the flavonol quercetin and the stilbene p-coumaric acid methyl ester with only quercetin showing inhibitory effect on P. ramosa. The activity of quercetin was compared with other two similar flavanoids, the flavone apigenin and the dihydroflavanol 3-O-acetylpadmatin extracted respectively from Lavandula stoechas and Dittrichia viscosa plants. In this comparative assay only 3-O-acetylpadmatin besides quercetin, showed inhibition activity of radicle growth while apigenin was inactive. These results indicated that the presence of two ortho-free hydroxy groups of C ring, like catechol, could be an important feature to impart activity while the carbon skeleton of B ring and substituents of both A and B rings are not essential. Besides reduction of radicle growth, haustorium induction was observed at the tip of P. ramosa radicles treated with quercetin which swelled and a layer of papillae was formed. Activity of quercetin on haustorium induction in P. ramosa was assayed in comparison with the known haustorium-inducing factor 2,6-dimethoxy-p-benzoquinone (DMBQ) and a three partial methyl ether derivatives semisynthetized from quercetin. Results indicated that P. ramosa haustorium was induced by DMBQ at concentrations of 1–0.5 mM and quercetin and its derivatives at concentration range 0.1–0.05 mM.

ACS Style

Mónica Fernández-Aparicio; Marco Masi; Alessio Cimmino; Susana Vilariño; Antonio Evidente. Allelopathic Effect of Quercetin, a Flavonoid from Fagopyrum esculentum Roots in the Radicle Growth of Phelipanche ramosa: Quercetin Natural and Semisynthetic Analogues Were Used for a Structure-Activity Relationship Investigation. Plants 2021, 10, 543 .

AMA Style

Mónica Fernández-Aparicio, Marco Masi, Alessio Cimmino, Susana Vilariño, Antonio Evidente. Allelopathic Effect of Quercetin, a Flavonoid from Fagopyrum esculentum Roots in the Radicle Growth of Phelipanche ramosa: Quercetin Natural and Semisynthetic Analogues Were Used for a Structure-Activity Relationship Investigation. Plants. 2021; 10 (3):543.

Chicago/Turabian Style

Mónica Fernández-Aparicio; Marco Masi; Alessio Cimmino; Susana Vilariño; Antonio Evidente. 2021. "Allelopathic Effect of Quercetin, a Flavonoid from Fagopyrum esculentum Roots in the Radicle Growth of Phelipanche ramosa: Quercetin Natural and Semisynthetic Analogues Were Used for a Structure-Activity Relationship Investigation." Plants 10, no. 3: 543.

Regular article
Published: 17 February 2021 in Chirality
Reads 0
Downloads 0

Sesquitepenoids inuloxins A–D, belonging to different subgroups, were isolated from Dittrichia viscosa and showed potential biocontrol of some parasitic plants as Pelipanche, Orobanche, and Cuscuta species. The absolute configurations of the first three inuloxins A–C were previously determined by using experimental and computational chiroptical spectroscopic methods. The absolute configuration of inuloxin D remains to be established. The bioactive inuloxin E, closely related to inuloxin D, was recently isolated from the same plant organic extract. The same relative configuration of inuloxin D was assigned to inuloxin E by comparison of their NMR spectroscopic data. The absolute configurations of inuloxin D and inuloxin E are suggested in this work by analysis of the experimental and predicted chiroptical properties of the 4‐O‐acetyl derivative of inuloxin D.

ACS Style

Jordan L. Johnson; Ernesto Santoro; Roukia Zatout; Ana G. Petrovic; Alessio Cimmino; Stefano Superchi; Antonio Evidente; Nina D. Berova; Prasad L. Polavarapu. Absolute configuration of seco‐eudesmanolide inuloxin D from experimental and predicted chiroptical studies of its 4‐ O ‐acetyl derivative. Chirality 2021, 33, 233 -241.

AMA Style

Jordan L. Johnson, Ernesto Santoro, Roukia Zatout, Ana G. Petrovic, Alessio Cimmino, Stefano Superchi, Antonio Evidente, Nina D. Berova, Prasad L. Polavarapu. Absolute configuration of seco‐eudesmanolide inuloxin D from experimental and predicted chiroptical studies of its 4‐ O ‐acetyl derivative. Chirality. 2021; 33 (5):233-241.

Chicago/Turabian Style

Jordan L. Johnson; Ernesto Santoro; Roukia Zatout; Ana G. Petrovic; Alessio Cimmino; Stefano Superchi; Antonio Evidente; Nina D. Berova; Prasad L. Polavarapu. 2021. "Absolute configuration of seco‐eudesmanolide inuloxin D from experimental and predicted chiroptical studies of its 4‐ O ‐acetyl derivative." Chirality 33, no. 5: 233-241.

Journal article
Published: 16 February 2021 in Biomolecules
Reads 0
Downloads 0

Fungi are among the biotic agents that can cause deterioration of building stones and cultural heritage. The most common methods used to control fungal spread and growth are based on chemical pesticides. However, the massive use of these synthetic chemicals produces heavy environmental pollution and risk to human and animal health. Furthermore, their use is time dependent and relies on the repetition of treatments, which increases the possibility of altering building stones and culture heritage through environmental contamination. One alternative is the use of natural products with high antifungal activity, which can result in reduced toxicity and deterioration of archeological remains. Recently, three fungal strains, namely Aspergillus niger, Alternaria alternata and Fusarium oxysporum, were isolated as damaging agents from the external tuff wall of the Roman remains “Villa of Poppea” in Oplontis, Naples, Italy. In this manuscript, three selected fungal metabolites, namely cyclopaldic acid, cavoxin and epi-epoformin, produced by fungi pathogenic for forest plants, were evaluated as potential antifungal compounds against the above fungi. Cavoxin and epi-epoformin showed antifungal activity against Asperigillus niger and Fusarium oxysporum, while cyclopaldic acid showed no activity when tested on the three fungi. The same antifungal activity was observed in vitro experiments on infected stones of the Neapolitan yellow tuff (NYT), a volcanic lithotype widely diffused in the archeological sites of Campania, Italy. This study represents a first step in the use of these two fungal metabolites to allow better preservation of artworks and to guarantee the conditions suitable for their conservation.

ACS Style

Marco Masi; Mariagioia Petraretti; Antonino De Natale; Antonino Pollio; Antonio Evidente. Fungal Metabolites with Antagonistic Activity against Fungi of Lithic Substrata. Biomolecules 2021, 11, 295 .

AMA Style

Marco Masi, Mariagioia Petraretti, Antonino De Natale, Antonino Pollio, Antonio Evidente. Fungal Metabolites with Antagonistic Activity against Fungi of Lithic Substrata. Biomolecules. 2021; 11 (2):295.

Chicago/Turabian Style

Marco Masi; Mariagioia Petraretti; Antonino De Natale; Antonino Pollio; Antonio Evidente. 2021. "Fungal Metabolites with Antagonistic Activity against Fungi of Lithic Substrata." Biomolecules 11, no. 2: 295.

Preprint
Published: 09 February 2021
Reads 0
Downloads 0

In the last decades, intensive crop management has involved excessive use of pesticides or fertilizers, compromising environmental integrity and public health. Accordingly, there has been worldwide pressure to find an eco-friendly and safe strategy to ensure agricultural productivity. Recently, Plant Growth-Promoting (PGP) rhizobacteria are receiving increasing attention as suitable biocontrol agents against agricultural pests. In the present study, 22 spore-forming bacteria were selected among a salt-pan rhizobacteria collection for their PGP traits and their antagonistic activity against the plant pathogen fungus Macrophomina phaseolina. Based on the higher antifungal activity, strain RHFS10, identified as Bacillus vallismortis, was furtherly examined and cell-free supernatants assays, column purification, and tandem mass spectrometry employed to purify and preliminarily identify the antifungal metabolites. Interestingly, the minimum inhibitory concentration assessed for the fractions active against M. phaseolina, resulted 10 times lower and more stable than the one estimated for the commercial fungicide pentachloronitrobenzene. These results suggest the use of B. vallismortis strain RHFS10 as a potential Plant Growth Promoting Rhizobacteria to efficiently control phytopathogenic fungus M. phaseolina, in alternative to chemical pesticides.

ACS Style

Stefany Castaldi; Claudia Petrillo; Giuliana Donadio; Fabrizio del Piaz; Alessio Cimmino; Marco Masi; Antonio Evidente; Rachele Isticato. Growth Promotion Function of Bacillus sp. Strains Isolated From Salt-Pan Rhizosphere and Their Biocontrol Potential Against Macrophomina Phaseolina. 2021, 1 .

AMA Style

Stefany Castaldi, Claudia Petrillo, Giuliana Donadio, Fabrizio del Piaz, Alessio Cimmino, Marco Masi, Antonio Evidente, Rachele Isticato. Growth Promotion Function of Bacillus sp. Strains Isolated From Salt-Pan Rhizosphere and Their Biocontrol Potential Against Macrophomina Phaseolina. . 2021; ():1.

Chicago/Turabian Style

Stefany Castaldi; Claudia Petrillo; Giuliana Donadio; Fabrizio del Piaz; Alessio Cimmino; Marco Masi; Antonio Evidente; Rachele Isticato. 2021. "Growth Promotion Function of Bacillus sp. Strains Isolated From Salt-Pan Rhizosphere and Their Biocontrol Potential Against Macrophomina Phaseolina." , no. : 1.

Journal article
Published: 08 February 2021 in Biomolecules
Reads 0
Downloads 0

Drechslera gigantea Heald & Wolf is a worldwide-spread necrotrophic fungus closely related to the Bipolaris genus, well-known because many member species provoke severe diseases in cereal crops and studied because they produce sesterpenoid phytoxins named ophiobolins which possess interesting biological properties. The unfolded protein response (UPR) is a conserved mechanism protecting eukaryotic cells from the accumulation of unfolded/misfolded proteins in the endoplasmic reticulum (ER). In plants, consolidated evidence supports the role of UPR in the tolerance to abiotic stress, whereas much less information is available concerning the induction of ER stress by pathogen infection and consequent UPR elicitation as part of the defense response. In this study, the infection process of D. gigantea in Arabidopsis thaliana wild type and UPR-defective bzip28 bzip60 double mutant plants was comparatively investigated, with the aim to address the role of UPR in the expression of resistance to the fungal pathogen. The results of confocal microscopy, as well as of qRT-PCR transcript level analysis of UPR genes, proteomics, microRNAs expression profile and HPLC-based hormone analyses demonstrated that ophiobolin produced by the fungus during infection compromised ER integrity and that impairment of the IRE1/bZIP60 pathway of UPR hampered the full expression of resistance, thereby enhancing plant susceptibility to the pathogen.

ACS Style

Simone Samperna; Angela Boari; Maurizio Vurro; Anna Salzano; Pierluigi Reveglia; Antonio Evidente; Angelo Gismondi; Antonella Canini; Andrea Scaloni; Mauro Marra. Arabidopsis Defense against the Pathogenic Fungus Drechslera gigantea Is Dependent on the Integrity of the Unfolded Protein Response. Biomolecules 2021, 11, 240 .

AMA Style

Simone Samperna, Angela Boari, Maurizio Vurro, Anna Salzano, Pierluigi Reveglia, Antonio Evidente, Angelo Gismondi, Antonella Canini, Andrea Scaloni, Mauro Marra. Arabidopsis Defense against the Pathogenic Fungus Drechslera gigantea Is Dependent on the Integrity of the Unfolded Protein Response. Biomolecules. 2021; 11 (2):240.

Chicago/Turabian Style

Simone Samperna; Angela Boari; Maurizio Vurro; Anna Salzano; Pierluigi Reveglia; Antonio Evidente; Angelo Gismondi; Antonella Canini; Andrea Scaloni; Mauro Marra. 2021. "Arabidopsis Defense against the Pathogenic Fungus Drechslera gigantea Is Dependent on the Integrity of the Unfolded Protein Response." Biomolecules 11, no. 2: 240.

Journal article
Published: 02 February 2021 in Antibiotics
Reads 0
Downloads 0

Chiliadenus lopadusanus Brullo is an Asteraceae plant species endemic to Lampedusa island, the largest island of the Pelage archipelago, Italy. The organic extract of its whole aerial parts, showing antibiotic activity against Staphylococcus aureus and Acinetobacter baumannii, wasfractionated employing bioguided purification procedures affording three main farnesane-type sesquiterpenoids. They were identified by spectroscopic methods (NMR and ESIMS data) as the (E)-3,7,11-trimethyldodeca-1,6,10-triene-3,9-diol, (E)-10-hydroxy-2,6,10-trimethyldodeca-2,6,11- trien-4-one and (E)-10-hydroxy-2,6,10-trimethyl-dodeca-6,11-dien-4-one, commonly named 9-hydroxynerolidol, 9-oxonerolidol, and chiliadenol B, respectively. These three sesquiterpenes, isolated for the first time from C. lopadusanus, were tested on methicillin-resistant S. aureus and A. baumannii showing antibacterial and antibiofilm activities. This plant could be used as a source to isolate secondary metabolites as potential new antibiotics.

ACS Style

Marco Masi; Emanuela Roscetto; Alessio Cimmino; Maria Catania; Giuseppe Surico; Antonio Evidente. Farnesane-Type Sesquiterpenoids with Antibiotic Activity from Chiliadenus lopadusanus. Antibiotics 2021, 10, 148 .

AMA Style

Marco Masi, Emanuela Roscetto, Alessio Cimmino, Maria Catania, Giuseppe Surico, Antonio Evidente. Farnesane-Type Sesquiterpenoids with Antibiotic Activity from Chiliadenus lopadusanus. Antibiotics. 2021; 10 (2):148.

Chicago/Turabian Style

Marco Masi; Emanuela Roscetto; Alessio Cimmino; Maria Catania; Giuseppe Surico; Antonio Evidente. 2021. "Farnesane-Type Sesquiterpenoids with Antibiotic Activity from Chiliadenus lopadusanus." Antibiotics 10, no. 2: 148.

Review article
Published: 25 January 2021 in Journal of Natural Products
Reads 0
Downloads 0

Two new penta- and tetrasubstituted cyclopentenones, named phaseocyclopentenones A and B (1 and 2), together with guignardone A (3), were isolated from Macrophomina phaseolina cultures. The phytopathogenic fungus was isolated from infected soybean tissues showing charcoal rot symptoms in Argentina. Charcoal rot is a devastating disease considering that soybean is one of the main legumes cultivated in the world. Phaseocyclopentenones A and B were characterized by 1D and 2D 1H and 13C NMR spectroscopic and HRESIMS spectrometric data and chemical methods as 4-benzoyl-3,4,5-trihydroxy-2-phenylcyclopent-2-enone and 3,5-dihydroxy-2,4-diphenylcyclopent-2-enone, respectively. The relative configuration of phaseocyclopentenones A and B was assigned by 1H and NOESY NMR methods, while their absolute configurations were assigned by electronic circular dichroism methods. When assayed on a nonhost plant (Solanum lycopersicum L.) by the leaf puncture assay, phaseocyclopentenones A and B and guignardone A showed phytotoxic activity, while only 1 and 2 were toxic when tested on cuttings of the same plant. No phytotoxicity or antifungal activity was detected for the three compounds on the host plant soybean (Glycine max L.) and against some of its fungal pathogens, namely, Cercospora nicotianae and Colletotrichum truncatum, also isolated from infected soybean plants in Argentina.

ACS Style

Marco Masi; Francisco Sautua; Roukia Zatout; Stefany Castaldi; Lorenzo Arrico; Rachele Isticato; Gennaro Pescitelli; Marcelo Anibal Carmona; Antonio Evidente. Phaseocyclopentenones A and B, Phytotoxic Penta- and Tetrasubstituted Cyclopentenones Produced by Macrophomina phaseolina, the Causal Agent of Charcoal Rot of Soybean in Argentina. Journal of Natural Products 2021, 84, 459 -465.

AMA Style

Marco Masi, Francisco Sautua, Roukia Zatout, Stefany Castaldi, Lorenzo Arrico, Rachele Isticato, Gennaro Pescitelli, Marcelo Anibal Carmona, Antonio Evidente. Phaseocyclopentenones A and B, Phytotoxic Penta- and Tetrasubstituted Cyclopentenones Produced by Macrophomina phaseolina, the Causal Agent of Charcoal Rot of Soybean in Argentina. Journal of Natural Products. 2021; 84 (2):459-465.

Chicago/Turabian Style

Marco Masi; Francisco Sautua; Roukia Zatout; Stefany Castaldi; Lorenzo Arrico; Rachele Isticato; Gennaro Pescitelli; Marcelo Anibal Carmona; Antonio Evidente. 2021. "Phaseocyclopentenones A and B, Phytotoxic Penta- and Tetrasubstituted Cyclopentenones Produced by Macrophomina phaseolina, the Causal Agent of Charcoal Rot of Soybean in Argentina." Journal of Natural Products 84, no. 2: 459-465.

Research article
Published: 15 January 2021 in Natural Product Research
Reads 0
Downloads 0

Since 1987, several cytochalasins were isolated from Phoma exigua var. heteromorpha, the causal agent of foliar blight disease of oleander (Nerium oleander L.), and chemically and biologically characterised. During the purification process of a large-scale production of cytochalasins A and B, necessary to continue the study on their anticancer activity, a metabolite having a different carbon skeleton compared to that of cytochalasans, was isolated. It was identified as terpestacin, a well-known toxic fungal stestertepenoid, isolated for the first time from P. exigua var. heteromorpha, by spectroscopic investigation (essentially 1D and 2D 1H and 13C-NMR and ESI MS) and optical methods in comparison with the literature data. Terpestacin and some its derivatives (including a natural one, fusaproliferin) were prepared and tested for their biological activity. Terpestacin and fusaproliferin had some inhibitory effects on seed germination of Phelipanche ramosa, whereas none of the compounds caused phytotoxic effects on weed leaves. Graphical Abstract

ACS Style

Marco Masi; Maria Chiara Zonno; Angela Boari; Maurizio Vurro; Antonio Evidente. Terpestacin, a toxin produced by Phoma exigua var. heteromorpha, the causal agent of a severe foliar disease of oleander (Nerium oleander L.). Natural Product Research 2021, 1 -7.

AMA Style

Marco Masi, Maria Chiara Zonno, Angela Boari, Maurizio Vurro, Antonio Evidente. Terpestacin, a toxin produced by Phoma exigua var. heteromorpha, the causal agent of a severe foliar disease of oleander (Nerium oleander L.). Natural Product Research. 2021; ():1-7.

Chicago/Turabian Style

Marco Masi; Maria Chiara Zonno; Angela Boari; Maurizio Vurro; Antonio Evidente. 2021. "Terpestacin, a toxin produced by Phoma exigua var. heteromorpha, the causal agent of a severe foliar disease of oleander (Nerium oleander L.)." Natural Product Research , no. : 1-7.

Research article
Published: 21 December 2020 in Natural Product Research
Reads 0
Downloads 0

Ophiobolin A is a secondary phytotoxic metabolite produced by some pathogenic fungal species responsible for severe plant diseases, considered to play a role in disease development and symptom appearance. Herein we investigated whether the phytotoxic activities of ophiobolin A against weed species could be improved by nanoencapsulation. Given the rapid natural degradation of the compound, it was hoped that nanoencapsulation would prolong the phytotoxic effects or enhance the bioactivity, thus leading to improved weed control capabilities. This article presents an assessment of the effectiveness of encapsulated ophiobolin A on 11 commonly found weed species, compared to the pure ophiobolin, to the particle alone, and a combination of mixed particles and ophiobolin A, by applying the solution droplets to both intact or injured leaf surface, on the adaxial or abaxial side. The bioassays showed the improved efficacy of the encapsulated ophiobolin, and the need for leaf lesions to diffuse the particles into the tissues. Graphical Abstract

ACS Style

Maurizio Vurro; Helen E. Townley; Rachel Morrison; Angela Boari; Marco Masi; Antonio Evidente. Augmented phytotoxic effect of nanoencapsulated ophiobolin A. Natural Product Research 2020, 1 -9.

AMA Style

Maurizio Vurro, Helen E. Townley, Rachel Morrison, Angela Boari, Marco Masi, Antonio Evidente. Augmented phytotoxic effect of nanoencapsulated ophiobolin A. Natural Product Research. 2020; ():1-9.

Chicago/Turabian Style

Maurizio Vurro; Helen E. Townley; Rachel Morrison; Angela Boari; Marco Masi; Antonio Evidente. 2020. "Augmented phytotoxic effect of nanoencapsulated ophiobolin A." Natural Product Research , no. : 1-9.

Journal article
Published: 17 December 2020 in Agriculture
Reads 0
Downloads 0

The allelopathic plant extracts can be applied as soil or foliar bioherbicides and are capable of suppressing germination and growth of several weeds, some of which are herbicide resistant. This study evaluated the allelopathic activity of the aqueous extract of aerial biomass of mugwort (Artemisia vulgaris L.) on seed germination and seedling growth of redroot pigweed (Amaranthus retroflexus L.) and maize (Zea mays L.), in order to be applied as a potential bioherbicide. The aqueous extract of mugwort was qualitatively examined for the presence of bioactive compounds and it was applied in a Petri dish and pot bioassays quantifying its effects on redroot pigweed and maize by non-linear regression analyses according the log-logistic model. The aqueous extract of mugwort showed the presence of several bioactive compounds with allelopathic activity, such as polysaccharides, organic acids, flavonoids and terpenoids. The aqueous extract of mugwort, at the concentrations from 7.5% to 10% w/v, were found to be the optimal concentration range since it is able to inhibit seed germination, seedling emergence and plant growth of redroot pigweed, without affecting seed germination and seedling emergence of maize, or rather, stimulating its radicle, mesocotyl and plant’s growth. EC90 values for the seed germination, radicle and hypocotyl length of redroot pigweed were in the order: 6.1% and 8.1%, 3.2% and 6.2%, 3.8% and 5.7% w/v of aqueous extract in the two repeated bioassays, respectively. Due to potential herbicidal activity against weeds and biostimulant action on the crops, this extract could be the ideal solution in an integrated weed management program, in order to suppress weeds, increasing competitive ability of crops.

ACS Style

Euro Pannacci; Marco Masi; Michela Farneselli; Francesco Tei. Evaluation of Mugwort (Artemisia vulgaris L.) Aqueous Extract as a Potential Bioherbicide to Control Amaranthus retroflexus L. in Maize. Agriculture 2020, 10, 642 .

AMA Style

Euro Pannacci, Marco Masi, Michela Farneselli, Francesco Tei. Evaluation of Mugwort (Artemisia vulgaris L.) Aqueous Extract as a Potential Bioherbicide to Control Amaranthus retroflexus L. in Maize. Agriculture. 2020; 10 (12):642.

Chicago/Turabian Style

Euro Pannacci; Marco Masi; Michela Farneselli; Francesco Tei. 2020. "Evaluation of Mugwort (Artemisia vulgaris L.) Aqueous Extract as a Potential Bioherbicide to Control Amaranthus retroflexus L. in Maize." Agriculture 10, no. 12: 642.