Intestinal parasitic infections such as amoebiasis, ascariasis, hookworm infection, and trichuriasis are the most common infections among non-human primates (NHPs). There are always the possibilities of transmission these parasites between humans and NHPs. Multiple groups of rhesus macaques (Macaca mulatta) live in the urban area of Kathmandu Valley near human settlements, however the gastrointestinal (GI) parasitic infections in those macaques are understudied. This study aimed to explore the GI parasites in free-ranging macaques from Pashupatinath, Swayambhunath, Tripureshwor, Nilbarahi temples and a group of captive rhesus macaques in the Central Zoo, Kathmandu. Fecal samples were collected from the macaques between October 2021 to September 2022 and assessed for parasites by the both wet mount method and concentration technique. There is high prevalence of GI parasite infection; out of 121 fecal samples examined, 87.6% of samples were positive. Six species of protozoans and eight species of helminths were identified from the fecal samples including the first report of Iodamoeba butschlii in monkeys of Nepal. Among the protozoan parasites, Entamoeba coli (54.71%) showed the highest prevalence followed by Balantioides coli (44.33%), E. histolytica (19.81%), and Iodamoeba butschlii (10%). Among the helminths, Trichuris spp. (31.13%) and Strongyloides spp. (31.13%) showed the highest prevalence followed by Hookworm (24.52%), and Strongyle spp. (23.58%). The likelihood ratio test suggested that the prevalence differed significantly with the seasons for Iodamoeba butschlii, Giardia spp., Strongyles spp., Hookworm, and Trichostrongylus spp. The prevalence of E. histolytica, E. coli, Iodamoeba. butschlii, Trichuris spp., Trichostrongylus spp., and Unknown spp.1 differed with sampling localities. The high prevalence of GI parasites found in the macaques living in the densely urbanized Kathmandu presents a potential threat to humans and warrants further study as well as increased education of the public and management of the human-macaque interface in the urban landscape of the Valley.

Staphylococcus aureus is a ubiquitous pathogen and colonizer in humans and animals. There are few studies on the molecular epidemiology of S. aureus in wild monkeys and apes. S. aureus carriage in rhesus macaques (Macaca mulatta) and Assam macaques (Macaca assamensis) is a species that has not previously been sampled and lives in remote environments with limited human contact. Forty Staphylococcus aureus isolates including 33 methicillin-susceptible S. aureus (MSSA) and seven methicillin-resistant S. aureus (MRSA) were characterized. Thirty-four isolates were from rhesus macaques and six isolates (five MSSA, one MRSA) were from Assam macaques. Isolates were characterized using StaphyType DNA microarrays. Five of the MRSA including one from Assam macaque were CC22 MRSA-IV (PVL+/tst+), which is a strain previously identified in Nepalese rhesus. One MRSA each were CC6 MRSA-IV and CC772 MRSA-V (PVL+). One MSSA each belonged to CC15, CC96, and CC2990. Six MRSA isolates carried the blaZ, while ten known CC isolates (seven MRSA, three MSSA) carried a variety of genes including aacA-aphD, aphA3, erm(C), mph(C), dfrA, msrA, and/or sat genes. The other 30 MSSA isolates belonged to 17 novel clonal complexes, carried no antibiotic resistance genes, lacked Panton–Valentine Leukocidin (PVL), and most examined exotoxin genes. Four clonal complexes carried egc enterotoxin genes, and four harbored edinB, which is an exfoliative toxin homologue.

The Ramaroshan Lake Complex (RLC) in Sudurpaschim Province, Nepal, is a Himalayan lake cluster that holds significant ecological, economic, religious, and esthetic importance. This study aimed to provide a comprehensive characterization of the hydrochemical properties of water within the RLC and assess its suitability for irrigation purposes. A total of 38 water samples were collected from seven different lakes of the complex. The physicochemical parameters and major ions were then analyzed. The water samples from the RLC were alkaline, and based on total hardness, they ranged from soft to moderately hard categories. The presence of major ions included the following: Ca²⁺ > Na⁺ > Mg²⁺ > K⁺ > Fe³⁺ > NH₄⁺ and HCO₃⁻ > Cl⁻ > SO₄²⁻ > NO₃⁻ > PO₄³⁻. The alkaline earth metals (Ca²⁺ and Mg²⁺) dominated the alkali metals (Na⁺ and K⁺) and weak acids (HCO₃⁻) dominated the strong acids (Cl⁻ and SO₄²⁻). The dominant hydrochemical facies of the lake water was a Ca-HCO₃ type indicating a calcium carbonate type of lithology. Carbonate rock weathering was the most dominant process in influencing the hydrochemistry of the water. A high ratio of (Ca²⁺+ Mg²⁺)/Tz⁺ and a lower ratio of (Na⁺ + K⁺)/Tz⁺ revealed the dominance of Ca²⁺ and Mg²⁺ resulting from carbonate weathering, with little contribution from silicate weathering. Different irrigation indices revealed the suitability of the RLC water for irrigation. The insights derived from this study are pivotal in safeguarding water quality and bolstering sustainability efforts. The study also furnishes foundational data crucial to an array of stakeholders including researchers and policymakers and significantly contributes to advancing water management strategies and fostering ecosystem conservation in the Himalayan freshwater lakes, particularly in the face of the overarching challenge posed by global climate change.

Wetlands are among the highly threatened ecosystems due to anthropogenic activities. The Ramaroshan Wetland Complex (RWC) of Achham District, Nepal is one of the high-altitude wetlands facing human induced degradation and loss. Herpetofauna are key bio-indicators of environmental health and habitat quality and are useful to assess habitat conditions of such threatened ecosystems. This study quantified the land use and land cover (LULC) change in the RWC and documented the diversity and distribution pattern of herpetofauna. The LULC in the area (13.94 Km²) was analyzed for 1989, 2000, 2010 and 2021 by supervised classification of remote sensing images. Surveys were conducted along 25 transects, each of 200 m in length and environmental variables were recorded for every observation of herpetofauna. The LULC analysis revealed an overall loss of 16% of the total water body between 1989 (0.25 Km²) and 2021 (0.21 Km²). Eleven species of herpetofauna (five amphibians and six reptiles) within five families and two orders (i.e., Anura and Squamata), were recorded with low diversity (H’ = 1.88312) and evenness (E = 0.3642) indices. The herpetofauna had a hump-shaped distribution along the elevation gradient with the highest richness and abundance at 2300 m asl. Amphibian abundance decreased with increasing distance to nearest water sources, whereas reptile abundance increased. Amphibians were more abundant in agricultural field and marsh land, whereas reptile abundance was higher around human settlements. Results indicate that the wetland area in the RWC is declining at an alarming rate and, in turn, might account for the low diversity and abundance of the herpetofauna.

Staphylococcus aureus is a widespread and common opportunistic bacterium that can colonise or infect humans as well as a wide range of animals. There are a few studies of both methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolated from monkeys, apes, and lemurs, indicating a presence of a number of poorly or unknown lineages of the pathogen. In order to obtain insight into staphylococcal diversity, we sequenced strains from wild and captive individuals of three macaque species (Macaca mulatta, M. assamensis, and M. sylvanus) using Nanopore and Illumina technologies. These strains were previously identified by microarray as poorly or unknown strains. Isolates of novel lineages ST4168, ST7687, ST7688, ST7689, ST7690, ST7691, ST7692, ST7693, ST7694, ST7695, ST7745, ST7746, ST7747, ST7748, ST7749, ST7750, ST7751, ST7752, ST7753, and ST7754 were sequenced and characterised for the first time. In addition, isolates belonging to ST2990, a lineage also observed in humans, and ST3268, a MRSA strain already known from macaques, were also included into the study. Mobile genetic elements, genomic islands, and carriage of prophages were analysed. There was no evidence for novel host-specific virulence factors. However, a conspicuously high rate of carriage of a pathogenicity island harbouring edinB and etD2/etE as well as a higher number of repeat units within the gene sasG (encoding an adhesion factor) than in human isolates were observed. None of the strains harboured the genes encoding Panton–Valentine leukocidin. In conclusion, wildlife including macaques may harbour an unappreciated diversity of S. aureus lineages that may be of clinical relevance for humans, livestock, or for wildlife conservation, given the declining state of many wildlife populations.

Background and Aim: In the past, the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) infections in both humans and animals has increased across Thailand. Staphylococcus argenteus has been associated with infections among humans, exotic pets, and livestock. Both species have been identified in non-human primate species from geographically diverse locations but not from non-human primates in Thailand. This study aimed to determine the presence of MRSA/ methicillin-susceptible S. aureus (MSSA) and S. argenteus isolates collected from buccal swab samples in Macaca fascicularis at Kosumpee Forest Park (KFP), Maha Sarakham, Northeast Thailand. Materials and Methods: Aseptic buccal swab samples were collected from 30 free-ranging macaques in November 2018. All isolates were tested using multiple biochemical tests and S. aureus latex slide agglutination test. Presumptive S. aureus isolates were tested for the presence of the mecA gene using polymerase chain reaction (PCR) assays. The isolates were phenotypically determined to be resistant to a β-lactam antibiotic using the disk diffusion method with a 30 μg cefoxitin disk. The isolates were analyzed by PCR for the non-ribosomal peptide synthetase (NRPS) gene to distinguish S. argenteus from S. aureus. Results: Fifteen macaques (50%) were colonized with S. aureus and 21 isolates were characterized. Three of the macaques carried both the MRSA and MSSA isolate. One animal carried both MRSA and S. argenteus isolate, and one animal carried only S. argenteus. The NRPS gene analysis confirmed that 2 isolates (9.52%) were S. argenteus and 19 isolates (90.48%) were S. aureus [five MSSA and 14 MRSA]. Conclusion: This study is the first to identify MRSA/MSSA and S. argenteus in wild free-ranging M. fascicularis from Thailand at the KFP in Maha Sarakham. This study is also the first report on the occurrence of S. argenteus carriage in M. fascicularis from Thailand.