Farm biosecurity management includes a set of practical measures used to prevent and limit the spread of infections to humans and animals. Infections, predominantly caused by zoonotic agents, often occur due to a lack of safety standards monitoring on farms, but also because of the use of inappropriate antimicrobial products leading to bacterial resistance, tolerance to biocides and the emergence antimicrobial-resistant germs. To date, research was mainly focused on studying the antimicrobial resistance in bacterial biofilms and the mechanisms involved in their occurrence. At molecular level, the limited diffusion of biocides in the biofilm matrix, enzyme-mediated resistance, genetic adaptation, efflux pumps, and levels of metabolic activity inside the biofilm are some of the investigated biological mechanisms which can promote antimicrobial resistance in biofilms were also investigated. Interventions, based on the identification of novel antimicrobial compounds, that would exclude the occurrence of bacterial tolerance, including essential oils (oregano, cloves), organic acids (tannic & oleic acid) and natural plant compounds (e.g. alkaloids, flavonoids, tannins and coumarins) were also extensively studied and reviewed given their effectiveness against pathogen-produced biofilms. The aim of this review was emphasize the importance of biosecurity and farm management practices and to assess their impact on bacterial biofilm formation. Furthermore, we present the recent intervention strategies aimed at reducing and combating the formation of bacterial biofilms in livestock farms.

This paper presents the development of a cost-effective automatic system for greenhouse environment control. The architectural and functional features were analyzed in the context of the realization of a controlled-environment agricultural system through all its stages: installation, deployment of the software, integration, maintenance, crop control strategy setup and daily operation of the grower. The proposed embedded platform provides remote monitoring and control of the greenhouse environment and is implemented as a distributed sensing and control network integrating wired and wireless nodes. All nodes were built with low-cost, low-power microcontrollers. The key issues that were addressed include the energy-efficient control, the robustness of the distributed control network to faults and a low-cost hardware implementation. The translation of the supervisory growth-planning information to the operational (control network) level is achieved through a specific architecture residing on a crop planning module (CPM) and an interfacing block (IB). A suite of software applications with flows and interfaces developed from a grower-centric perspective was designed and implemented on a multi-tier architecture. The operation of the platform was validated through implementation of sensing and control nodes, application of software for configuration and visualization, and deployment in typical greenhouses.

In our days consumers are interested and visibly reoriented towards natural/local/organic products. Starting from this specificity, the development of rural tourism can come as a possible sustainable response to be implemented in many of the rural areas, such an example being the Moieciu area subject to our analysis. The main objective of this paper is to achieve an analysis of rural tourism activity’s development stage from Moieciu area, from the tourist’s perspective, using case study method and as “main tool of research” the questionnaire. Thus, several research-oriented goals followed are: establishing the arguments for practicing tourism in rural areas; describing the development of Bran-Moieciu area from economic, agricultural and tourist point of view; identifying the reasons for which Moieciu area is a place where tourism can ensure sustainable development. The measure of appreciation of this area by the tourist is analyzed through a face-to-face questionnaire, having as purpose to identify: the capitalization level of local resources through rural tourism activities; the extent to which local resources are present in the product consumed by tourists; the satisfaction degree and expectations regarding the tourist product; the connection between tourism and area development in terms of satisfaction. Based on the information obtained some proposals/possible directions will be proposed to transform the area into “a possible model of sustainable rural tourism development”.

Many images of agricultural crops are made at different times of the day, images with different spectral information about the same crop in relation to conditions when the picture was taken. A set of 30 digital images of a wheat crop in the BBCH 3-Stem elongation code 32–33 stage was captured between 9 am and 14 (UTC+3), in the 0°–180° variation range of the image acquisition angle on the E-W axis (cardinal directions). A high variation of the spectral data given by the combination of the hour (h) and angle (a) at which the images were captured was found. The interdependence relationship between the analyzed parameters (r, g, and b), and the time (t) and the angle (a) of image acquisition was assessed with the linear correlation coefficient. By calculating the roots of the mathematical expressions of the correlation coefficients dependence on the angles (a) or times of day (t), the optimal angle and time were determined as a combination of the two variables for capturing images and obtaining optimal r_o, g_o, b_o values. The correction coefficients of the normalized r, g, and b values obtained out of the optimal field were determined. To this end, the multiplication of the r(a,t), g(a,t), and b(a,t) values with the ρ_a,t, γ_a,t, and β_a,t correction coefficients was suggested to reach the optimal values for sustainable decisions.

Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis can colonize the tooth root canals, adhere to dentin walls, and frequently cause periodontitis in dogs. Bacterial periodontal diseases are common in domesticated pets, causing severe oral cavity inflammation and a strong immune response. This study investigates the antioxidant effect of a natural antimicrobial mixture (Auraguard—Ag) on the ability of S. aureus, S. pyogenes and E. faecalis to infect primary canine oral epithelial cells as well as its impact on their virulence factors. Our data show that a concentration of 0.25% Ag is sufficient to inhibit the growth of all three pathogens, whereas a concentration of 0.5% will become bactericidal. The sub-inhibitory concentration of 0.125% Ag reveals that the antimicrobial mixture can significantly reduce biofilm formation and exopolysaccharide production. The impact on these virulence factors was further translated into a significantly reduced ability to infect primary canine oral epithelial cells and restore epithelial tight junctions, with no impact on the epithelial cell viability. The post-infection inflammatory cytokines (IL-1β and IL-8) and the COX-2 mediator were also reduced both in mRNA and protein expression levels. The oxidative burst, detected upon infection, was also decreased in the presence of Ag, as our results show a significant decrease in H₂O₂ released by the infected cells. We show that inhibition of either NADPH or ERK activity will result in a downregulation of COX-2 expression and lower levels of H₂O₂ in infected cells. Conclusively, our study shows that natural antimicrobials reduce pro-inflammatory events, post infection, through an antioxidative mechanism that involves the downregulation of the COX-2 mediator via the inactivation of ERK in the absence of H₂O₂. As a result, they significantly reduce the risk of secondary bacterial infections and host oxidative stress caused by Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis accumulation in biofilms in an in vitro canine oral infection model.

Legionella pneumophila is responsible for causing Legionnaires’ disease and Pontiac fever, also known as legionellosis. The aim of this study was to investigate the mechanistic effect of a mixture of natural antimicrobials (Citrox BCL) in preventing L. pneumophila biofilm formation and reducing its in vitro virulence. The minimum inhibitory concentrations were detected at 0.06%, and the MBC was established at 0.125%. Based on the growth curve profile, the sub-inhibitory concentration of 0.02% was further used to study the mechanistic implications in the absence of a cytotoxic effect on A549 cells. At 24 h post-infection, Citrox BCL reduced (p = 0.005) the intracellular growth of L. pneumophila when the A549 cells or the bacteria were pre-treated with 0.02% Citrox BCL. This result was replicated when Citrox BCL was added during the 24 h infection assay leading to a reduction in intracellular growth (p = 0.003). Herein we show that at the sub-inhibitory concentration of 0.02%, Citrox CBL lowers the ROS levels in infected A549 cells and causes a 45% reduction in L. pneumophila EPS production, a reduction associated with the decline in biofilm formation. Overall, our results corroborate the low c-di-GMP production with the decrease in biofilm formation and low EPS levels. The low EPS levels seemed to be caused by the downregulation of the tatB and tatC gene expressions. Moreover, inhibition of pvcA and pvcB gene expressions, leading to lower siderophore levels, suggests that Citrox BCL reduces the ability of L. pneumophila to sequester iron and reduce biofilm formation through iron starvation.