THE EFFECT OF DIFFERENT DIETARY LEVELS OF HYBRID RYE AND XYLANASE ADDITION ON THE PERFORMANCE AND EGG QUALITY IN LAYING HENS. British Poultry Science. Accepted 21 February 2019.

Thirty clinically healthy Holstein-Friesian cows underwent twice daily machine milking and ultrasonographic examinations of the udder just prior to and after milking. Digital ultrasonographic images of each udder quarter were subjected to computer-assisted echotextural analyses to obtain mean numerical pixel values (NPVs) and pixel heterogeneity (PSD) of the mammary gland parenchyma. The average milk yield and pH were higher (p < 0.05) in the morning, whereas crude fat, total solids, solids non-fat and citric acid content were higher (p < 0.05) during the evening milking period. Mean NPVs and PSDs of the mammary gland parenchyma were greater (p < 0.05) after than before milking. There were significant correlations among echotextural characteristics of the udder and protein percentage, lactose content and freezing point depression determined in the milk samples collected in the morning and crude protein, casein, lactose and solids non-fat in the evening. Our results can be interpreted to suggest that computerized analysis of the mammary gland ultrasonograms has the makings of a technique for estimating non-fat milk constituents in cows. However, future validating studies are necessary before this method can be employed in commercial settings and research. Moreover, significant inter-quarter differences in udder echogenicity may necessitate further echotextural studies of separate quarters.

The aim of the study was to determine the effect of the method of fragmentation of hybrid rye and maize grain on digestibility parameters for ruminants. Varying degrees of fragmentation – none (whole grains; WG), crushed (CG) or ground to pass through a 4.0 (GG4.0) or 1.5 mm (GG1.5) screen – were estimated by the in sacco nylon bag technique, in vitro true digestibility (IVTD), and the in vitro gas production (GP) technique. WG, CG, GG4.0 and GG1.5 were categorized as none, slight, moderate and extensive fragmentation of the grain kernel, respectively. Three non-lactating cows fitted with ruminal and duodenal cannulas were used to determine the effective rumen degradability (ERD) and intestinal and total tract digestibility (ID and TTD, respectively) of dry matter (DM), crude protein (CP) and starch. IVTD was determined with an ANKOM Daisy ^II Incubator, and GP and in vitro organic matter digestibility were determined with an ANKOM ^RF Gas Production System. Dry matter, CP and starch of hybrid rye grain were rapidly degraded in the rumen, and this degradability as well as ID and TTD was only marginally affected by the method of kernel fragmentation; however, among the methods used, the ERD of GG4.0 rye was the lowest. On the other hand, the greater the degree of kernel fragmentation, the higher ERD, ID and TTD were obtained for the DM, CP and starch of maize grain. In summary, rye grain is more susceptible to fermentation in the rumen than maize grain, but the means of grain processing may alter the rate, extent and site of their digestion, particularly for maize grain.

Six wether sheep with ruminal and duodenal cannulas were used in a replicated 3 × 3 Latin square to determine the effect of rye grain (from an open-pollinated or a hybrid cultivar) and maize grain from hybrid cultivar inclusion in the diet on ruminal fermentation parameters and nutrient digestion in different gastrointestinal tract compartments. The experimental diets consisted of meadow hay (800 g/day) and a concentrate mixture (300 g/day) that contained rye grain from an openpollinated cultivar (OPRG), rye grain from a hybrid cultivar (HRG), or maize grain from a hybrid cultivar (MG) as a main energy source. Each experimental period lasted 17 days and consisted of 10 days for diet adaptation and 7 days for data and sample collection. Ruminal fermentation (pH, short-chain fatty acids, and ammonia concentration) and nutrient digestion up to the duodenum (in reticulorumen, omasum, and abomasum), in the intestine, as well as in the total digestive tract was investigated. Mean ruminal pH was higher for MG compared to HRG and OPRG (P<0.01) but did not differ between HRG and OPRG (6.45, 6.35, and 6.29 for MG, HRG, and OPRG, respectively). Treatment did not affect short-chain fatty acid concentration in the rumen, except for the molar proportions of valeric acid and of isovaleric acid of the total short-chain fatty acids, which was or tended to be higher (P≤0.06) for MG compared to HRG and OPRG. Less starch was digested in the gastrointestinal tract up to the duodenum and more in the intestine for MG compared to HRG and OPRG (P=0.01); however, total tract nutrient digestibility did not differ between treatments. In conclusion, usage of rye grain in sheep diets resulted in a lower ruminal pH compared to maize grain usage. No differences for ruminal fermentation, nutrient digestion up to the duodenum, in the intestine, as well as in the total digestive tract between rye grain from open-pollinated and hybrid cultivar usage in sheep diets were found.

Grain from traditional varieties of rye is not commonly used in dairy cattle nutrition. However, new hybrid varieties of rye currently available are characterized by some nutritional and agrotechnical benefits. This paper deals with the hypothesis that rye grain derived from a hybrid variety may be an alternative for maize grain in diets for dairy cattle. Sixteen lactating Polish Holstein- Friesian cows were divided into two groups according to their parity (8 primi- and 8 multiparous), stage of lactation (106 ± 30 days after calving) and milk yield (34 ± 4 kg/day). Cows were fed a total mixed ration (TMR) containing grass silage and whole crop maize silage and 29.2% of the concentrate (in dry matter). The latter contained approximately 48% (as fed) of either maize grain (M) or hybrid rye grain (HR) as a main source of cereal grain. Experimental diets were fed for 9 weeks. The use of HR as a substitute for M did not affect (P>0.05) dry matter intake and milk yield. There were no differences between treatments in the content of milk solids, amino acids, and fatty acid profile. However, substituting M by HR positively influenced composition of milk protein fractions by increasing the proportion of α-casein (37.0 vs 39.7%; P<0.01) and к-casein (6.5 vs 7.3%; P=0.02) as well as decreasing the proportion of β-casein (28.8 vs 27.8%; P=0.02) and sensory characteristics of the milk (body and texture, and taste; P<0.05). In turn, the composition of the diet did not affect the technological suitability for processing of milk fat (acid and peroxide number, melting and solidification temperature), rennet coagulation time, heat stability or titratable acidity. This study has shown that hybrid rye grain may be an alternative for maize grain in a TMR based on grass and whole maize silage for mid-lactation dairy cows. Further studies are needed with higher proportion of hybrid rye grain in TMR or with other roughages used in a basal diet to fully determine efficiency of hybrid rye grain use in diets for dairy cows.