A high content of non-starch polysaccharides (NSP), namely arabinoxylans (AX), in rye is a reason for the potential adverse effect of this grain on intestinal functions, gut microflora, absorption of nutrients and performance indices. As such, the use of rye grain in intensively produced poultry diets is limited. However, recently developed new types of hybrid rye are characterised not only by increased yield potential, resistance to fungus and pests and low production costs, but also the content of antinutritive substances may be reduced in these varieties. The aim of this paper is to discuss the mechanisms of NSP effects in the digestive tract, as well as to review the results of recent studies on the use of rye in poultry nutrition. Based on the literature data, it can be concluded that the use of new hybrid rye varieties with decreased NSP concentration and NSP-hydrolising enzymes may be a way of increasing the share of rye grain in poultry diets.

In this paper, we present a study on the evaluation of the effect of xylanase addition to a diet with an increasing content of modern hybrid rye (Brasetto variety) on the performance indices and viscosity of small intestine content in broiler chickens. A total of 560 1-day-old male Ross 308 chickens were randomly assigned to 1 of 10 treatments, each comprising 7 replicate cages, with 8 male birds per cage. A 5 × 2 factorial arrangement was employed, with five dietary levels of ground rye (0%, 5%, 10%, 15%, and 20%). All the diets were either unsupplemented or supplemented with xylanase (200 mg/kg of feed; with minimum xylanase activity 1000 FXU/g). In the starter rearing period (1–21 days of age), the inclusion of rye (without xylanase supplementation) to the diet, even at the lowest dietary level (5%), negatively affected body weight gain (p < 0.05); there was no effect on feed intake and feed conversion ratio. In older chickens (the grower-finisher rearing period; 22–42 days of age), none of the dietary levels of rye (5–20%) affected growth performance indices. Similarly, no significant effect of increasing dietary level of rye was found for the entire rearing period (1–42 days of age). Diet supplementation with xylanase improved body weight gain and feed conversion ratio in chickens from 1 to 21 days of age. No positive effect of enzyme was found in older birds. No significant effects of the experimental factors used were noticed on the results of slaughter analysis, i.e., the carcass yield, breast meat yield, abdominal fat, and relative weight of the liver and gizzard. A high dietary concentration of rye (20%) increased the viscosity of small intestine content (p < 0.05); however, diet supplementation with xylanase significantly alleviated this effect. The findings of this experiment indicated that modern hybrid rye grain may be used at a 20% dietary level in broiler diets during the second feeding phase, i.e., from 22 to 42 days of age, without any detrimental influence on growth performance indices, while enzyme (xylanase) positively affected body weight gain and feed conversion ratio in younger chicks (1–21 days of age).

Nanoparticles are increasingly popular in numerous fields including electronics, optics and medicine (vaccines, tissue engineering, microsurgery, genomics and cancer therapies). The most widely used nanoparticles in biomedical applications are those designed by man. Scientists have obtained many types of silica nanoparticles with defined shape and chemical composition, but different properties and applications. Nanoparticles include particles with at least one dimension ranging from 1–100 nm. Silica nanoparticles (Sn), reaching values from several dozen to several hundred m²/g, have unique physicochemical properties due to their porous structure and well-developed specific surface. Currently, the use of Sn in animal nutrition, with a focus on gastrointestinal tract function, is of great interest.

THE EFFECT OF DIFFERENT DIETARY LEVELS OF HYBRID RYE AND XYLANASE ADDITION ON THE PERFORMANCE AND EGG QUALITY IN LAYING HENS. British Poultry Science. Accepted 21 February 2019.

The main aim of the study was to evaluate the effect of two experimental feed additives based on kaolin clay on piglets’ health and microbiota status. The experiment was divided into 2 parts – from birth up to weaning (28 d) and later after weaning up to 70 d of age. Eighteen litters of piglets with sows were divided into 3 groups: control, A and B. The animals from experimental group A were supplemented with kaolin clay (100%), while the animals from group B received kaolin clay enriched with dried pomace of chokeberry and fructooligosaccharides. We noted an improvement in blood parameters related to anaemia, which were significantly better in the experimental group B in comparison to the control and A group (haematocrit; 37.67 and 37.40 vs 39.65%; P<0.0005). The dietary treatments during pre-weaning time influenced the increase of the E. coli strain in the colon and jejunum and had no effect on lowering the population of Salmonella and Shigella in the colon. However, it affected the quantity of Salmonella and Shigella in the jejunum in both A and B (-20%) groups. The rise of commensal bacteria Lactobacillus (+2.3 and +10%) and Bacteroides (+5.82 and +5.11%) was observed in groups A and B in the colon. This effect was not present in the jejunum.

Background: Preclinical and clinical studies suggest that zinc deficiency and chronic stress contribute to depressive symptoms. Our study explores the intricate relationship between these factors by examining their physiological and biochemical effects across various organs in C57Bl/6J mice. Methods: The mice were divided into four groups: control, chronic restraint stress for 3 weeks, a zinc-restricted diet (<3 mg/kg) for 4 weeks, and a combination of stress and zinc restriction. Mice spleen and thymus weights were measured, and hematoxylin–eosin staining was conducted for liver and intestinal morphometry. Moreover, metallothionein (MT-1, MT-2, and MT-3), zinc transporter (ZnT-1), oxidative stress markers (TBARS, SOD, and GSH-Px), and zinc, iron, and copper concentrations in the liver were evaluated. Immunohistochemical analysis of the jejunum for ZIP1 and ZIP4 was also performed. Conclusions: Our findings reveal that dietary zinc restriction and chronic stress induce structural changes in the intestines and immune organs and impact metallothionein expression, oxidative stress, and liver iron and copper homeostasis.