Yoga has been shown to be a simple and economical therapeutic modality that may be considered as a beneficial adjuvant for type 2 diabetes mellitus. This study investigated the impact of Hatha yoga and conventional physical training (PT) exercise regimens on biochemical, oxidative stress indicators and oxidant status in patients with type 2 diabetes.

Alzheimer’s disease is a progressive, clinically heterogeneous, and particularly complex neurodegenerative disease characterized by a decline in cognition. Over the last two decades, there has been significant growth in the investigation of cerebrospinal fluid (CSF) biomarkers for Alzheimer’s disease. This review presents current evidence from many clinical neurochemical studies, with findings that attest to the efficacy of existing core CSF biomarkers such as total tau, phosphorylated tau, and amyloid-β (Aβ₄₂), which diagnose Alzheimer’s disease in the early and dementia stages of the disorder. The heterogeneity of the pathophysiology of the late-onset disease warrants the growth of the Alzheimer’s disease CSF biomarker toolbox; more biomarkers showing other aspects of the disease mechanism are needed. This review focuses on new biomarkers that track Alzheimer’s disease pathology, such as those that assess neuronal injury (VILIP-1 and neurofilament light), neuroinflammation (sTREM2, YKL-40, osteopontin, GFAP, progranulin, and MCP-1), synaptic dysfunction (SNAP-25 and GAP-43), vascular dysregulation (hFABP), as well as CSF α-synuclein levels and TDP-43 pathology. Some of these biomarkers are promising candidates as they are specific and predict future rates of cognitive decline. Findings from the combinations of subclasses of new Alzheimer’s disease biomarkers that improve their diagnostic efficacy in detecting associated pathological changes are also presented.

Succinic acid (SA) and oleic acid (OA) are the primary hypoglycaemic agents in Desmodium canum, a plant traditionally employed for its potential health benefits. The synergy of the two organic acids exhibits potency in retarding blood glucose levels (BGL) in euglycaemic Sprague Dawley (S-D) rats following a single-dose administration. A cocktail of the two compounds is being investigated for its antidiabetic properties in fructose-fed streptozotocin (STZ)-induced diabetic rats. Eighteen type 2 diabetic S-D rats were divided into 3 groups and treated for 28 d with either the cocktail (OA + SA, 800 mg/kg body weight [BW]), glibenclamide (10 mg/kg BW), or vehicle (10% polysorbate 20). Another 12 S-D rats served as euglycaemic animals and were divided into two groups, fed either the cocktail (OA + SA, 800 mg/kg BW) or vehicle. Changes in BW, blood pressure (BP), BGL, water and food consumption, serum insulin, serum glucagon and insulin resistance (IR) were monitored. Treatment with the cocktail showed no change in euglycaemic animals; however, there was a significant reduction in the BGL of diabetic treated animals when compared with diabetic control (14.48 ± 1.92 vs. 25.56 ± 1.38 mM; ). Quantitative insulin sensitivity check index (QUICKI) and glucose/insulin (G/I) scores for IR indicated an improvement in insulin response in the diabetic treated animals. Additionally, there was a noticeable reduction in food and water consumption when compared with diabetic control animals, which was accompanied by a reduction in weight. Overall, the cocktail elicited antidiabetic properties and may serve an important therapeutic role as a nutritional supplement in type 2 diabetics.

Background: Pre-eclampsia (PE) complicates approximately 5-7% of all pregnancies. This study investigates the effects of S-nitroso-N-acetylpenicillamine (SNAP) and S-nitrosoglutathione (GSNO) on the classical features of PE. Materials and Methods: On day 14 of gestation, female Sprague-Dawley rats were separated into five groups and treated intravenously for 7 days as follows: (i) 0.3 mL 0.9% saline (control, n = 11); (ii) 50 mg/kg Body Weight (BW) N-nitro-L-arginine methyl ester (L-NAME) in 0.3 mL saline ( n = 10); (iii) 50 mg/kg BW L-NAME and 8 mg/kg BW GSNO in 0.15 mL saline ( n = 6); (iv) 50 mg/kg BW L-NAME in 0.15 mL saline and 8 mg/kg BW SNAP in 0.15 mL DMSO ( n = 9); and (v) 0.15 mL DMSO and 0.15 mL saline (SNAP control, n = 7). Blood pressures were measured on day 14 through day 20, a 4-h urine sample was taken on day 20, and animals were sacrificed on day 21. Pups were counted and weighed individually. Results: SNAP and GSNO significantly decreased systolic, diastolic, and mean arterial pressures in PE-induced rats from day 14 through day 20 ( P < 0.05). Pup weights in SNAP and GSNO groups were higher than in L-NAME group but lower than in controls ( P ≤ 0.001). SNAP and GSNO partially reversed growth retardation. Conclusion: Elevated blood pressure, proteinuria, and intrauterine growth restriction associated with PE were induced in Sprague-Dawley rats using L-NAME. These were partially reversed with the use of GSNO and SNAP. The mechanism of action of these S-nitrosothiols (RSNOs) should be further explored.

Nutritional supplements are gaining traction for their effects in mitigating the impacts of various health conditions. In particular, many supplements are being proposed to reduce the impacts of type 2 diabetes (T2D), a metabolic condition that has reached global epidemic proportions. Recently, a supplement of oleic acid (OA) and succinic acid (SA; 1 : 1, w/w) was reported to improve glycaemic control in type 2 diabetic (T2D) Sprague–Dawley (S‐D) rats through ameliorating insulin release and sensitivity. Here, we investigate the effects of the supplement (OA and SA) on hepatic and pancreatic function in T2D S‐D rats. Eighteen (18) S‐D rats were rendered diabetic and were divided into three equal groups: diabetic control, diabetic treatment, and diabetic glibenclamide. Another 12 S‐D rats were obtained and served as the normal groups. The animals were treated daily with the vehicle, OA and SA (800 mg/kg body weight (bw); 1 : 1), or glibenclamide (10 mg/kg bw) which served as the positive control. The findings indicated that treatment with the supplement resulted in a 35.69 ± 4.22% reduction (p = 0.006) in blood glucose levels (BGL). Analysis of hepatic enzymes depicted that the nutritional supplement reduced the activity of the gluconeogenesis enzyme, glucose‐6‐phosphatase (G6P) while improved the activity of catabolic enzymes such as glucose‐6‐phosphate dehydrogenase (G6PD) and pyruvate kinase (PK). Furthermore, the supplement attenuated oxidative stress through restoration of catalase (CAT) and superoxide dismutase (SOD), while reducing malondialdehyde (MDA) levels. Finally, the supplement showed no liver or kidney toxicity and improved the size and number of pancreatic islets of Langerhans, indicating its potential application in treating T2D. The study highlighted that a supplement of the two organic acids may be beneficial in reducing the rate of pathogenesis of type 2 diabetes. Therefore, it may offer therapeutic value as a dietary or nutritional supplement in the approach against diabetes and its complications.

Introduction: Desmodium canum (Strong Back) is deemed a versatile traditional medicine, where it is used to treat diabetes, hypertension, asthma and erectile dysfunction. Aim: To identify the various phytochemicals present within extracts of D. canum, their antioxidant capabilities and their effects on blood glucose levels, haemodynamic parameters and testosterone levels in healthy Sprague-Dawley (S-D) rats. Method: Crude extracts were obtained using hexane, ethyl acetate and methanol. These were analysed for various phytochemicals and their antioxidant potential assessed using the 2,2-diphenyl-1picrylhydrazyl (DPPH) method. The extracts were investigated for hypoglycaemic potential using the Oral Glucose Tolerance Test (OGTT), where extracts were administered intravenously (50 mg/kg BW) to fasted rats and their blood glucose readings monitored at 30 min intervals. The hypotensive effect of the extracts were also investigated where rats were administered intravenously at 50 mg/kg BW. These haemodynamic parameters were monitored using the CODA 6 machine at 5 min intervals for a total of 20 min. Additionally, the effect on testosterone level was investigated in male rats where extracts were administered daily by oral gavage. Serum testosterone levels were then determined using an ELISA kit. Results: The different extracts showed varying phytochemical and pharmacological properties. The methanol extract showed antioxidant capabilities, while the ethyl acetate extract showed significant hypoglycaemic and hypotensive effects when compared with the control. The hexane extract showed significant activity in increasing the testosterone when compared with the control. Conclusion: D. canum extracts showed significant pharmacological activities in normal Sprague- Dawley rats.