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Y Wang
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China

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Journal article
Published: 28 June 2021 in Animals
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The fertility of female animals is negatively correlated with increasing chronological age. In aging broiler breeder hens, there is a decline in the functionality of the ovary and liver accompanied by hormonal or endocrine changes, a reduction in antioxidant capacity, and a decrease in folliculogenesis. Therefore, improving the reproductive function in aging breeder hens using dietary strategies is of great concern to the poultry breeder. This study evaluated the capacity of dietary quercetin (Q), vitamin E (VE), and their combination (Q + VE) to promote follicle development and attenuate organ inflammation by improving the antioxidant capacity of aging breeder hens. In this study, 400 broiler breeder hens (Tianfu broilers breeder hens, 435 days old) were allotted into four groups (100 birds each) with four replicates each (25 birds each). They were fed diets containing Q (0.4 g/kg), VE (0.2 g/kg), Q + VE (0.4 g/kg + 0.2 g/kg), and a basal diet for 10 weeks. The results showed that Q + VE improved the organ characteristics (p< 0.05), and also that Q + VE showed protective effects on the liver against injury, as well as increasing the antioxidant capacity of the liver, serum, and ovary (p< 0.05). Furthermore, liver lipid synthesis was increased remarkably, as indicated by the changes in triglyceride levels in hens fed Q + VE (p< 0.05). Levels of E2, FSH, and LH, their receptors, and mRNAs related to yolk precursor synthesis were increased by the Q + VE (p< 0.05). Therefore, the combination of quercetin and vitamin E synergistically promotes and regulates the transportation and exchange of synthetic substances among the liver–blood–ovary alliances to ensure the synchronous development and functional coordination between the liver and ovary in aging breeder hens.

ACS Style

Felix Amevor; Zhifu Cui; Xiaxia Du; Zifan Ning; Gang Shu; Ningning Jin; Xun Deng; Yaofu Tian; Zhichao Zhang; Xincheng Kang; Dan Xu; Guishuang You; Yao Zhang; Diyan Li; Yan Wang; Qing Zhu; Xiaoling Zhao. Combination of Quercetin and Vitamin E Supplementation Promotes Yolk Precursor Synthesis and Follicle Development in Aging Breeder Hens via Liver–Blood–Ovary Signal Axis. Animals 2021, 11, 1915 .

AMA Style

Felix Amevor, Zhifu Cui, Xiaxia Du, Zifan Ning, Gang Shu, Ningning Jin, Xun Deng, Yaofu Tian, Zhichao Zhang, Xincheng Kang, Dan Xu, Guishuang You, Yao Zhang, Diyan Li, Yan Wang, Qing Zhu, Xiaoling Zhao. Combination of Quercetin and Vitamin E Supplementation Promotes Yolk Precursor Synthesis and Follicle Development in Aging Breeder Hens via Liver–Blood–Ovary Signal Axis. Animals. 2021; 11 (7):1915.

Chicago/Turabian Style

Felix Amevor; Zhifu Cui; Xiaxia Du; Zifan Ning; Gang Shu; Ningning Jin; Xun Deng; Yaofu Tian; Zhichao Zhang; Xincheng Kang; Dan Xu; Guishuang You; Yao Zhang; Diyan Li; Yan Wang; Qing Zhu; Xiaoling Zhao. 2021. "Combination of Quercetin and Vitamin E Supplementation Promotes Yolk Precursor Synthesis and Follicle Development in Aging Breeder Hens via Liver–Blood–Ovary Signal Axis." Animals 11, no. 7: 1915.

Journal article
Published: 26 May 2021 in Genes
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The proliferation and differentiation of skeletal muscle satellite cells (SMSCs) play an important role in the development of skeletal muscle. Our previous sequencing data showed that miR-21-5p is one of the most abundant miRNAs in chicken skeletal muscle. Therefore, in this study, the spatiotemporal expression of miR-21-5p and its effects on skeletal muscle development of chickens were explored using in vitro cultured SMSCs as a model. The results in this study showed that miR-21-5p was highly expressed in the skeletal muscle of chickens. The overexpression of miR-21-5p promoted the proliferation of SMSCs as evidenced by increased cell viability, increased cell number in the proliferative phase, and increased mRNA and protein expression of proliferation markers including PCNA, CDK2, and CCND1. Moreover, it was revealed that miR-21-5p promotes the formation of myotubes by modulating the expression of myogenic markers including MyoG, MyoD, and MyHC, whereas knockdown of miR-21-5p showed the opposite result. Gene prediction and dual fluorescence analysis confirmed that KLF3 was one of the direct target genes of miR-21-5p. We confirmed that, contrary to the function of miR-21-5p, KLF3 plays a negative role in the proliferation and differentiation of SMSCs. Si-KLF3 promotes cell number and proliferation activity, as well as the cell differentiation processes. Our results demonstrated that miR-21-5p promotes the proliferation and differentiation of SMSCs by targeting KLF3. Collectively, the results obtained in this study laid a foundation for exploring the mechanism through which miR-21-5p regulates SMSCs.

ACS Style

Donghao Zhang; Jinshan Ran; Jingjing Li; Chunlin Yu; Zhifu Cui; Felix Amevor; Yan Wang; Xiaosong Jiang; Mohan Qiu; Huarui Du; Qing Zhu; Chaowu Yang; Yiping Liu. miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken. Genes 2021, 12, 814 .

AMA Style

Donghao Zhang, Jinshan Ran, Jingjing Li, Chunlin Yu, Zhifu Cui, Felix Amevor, Yan Wang, Xiaosong Jiang, Mohan Qiu, Huarui Du, Qing Zhu, Chaowu Yang, Yiping Liu. miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken. Genes. 2021; 12 (6):814.

Chicago/Turabian Style

Donghao Zhang; Jinshan Ran; Jingjing Li; Chunlin Yu; Zhifu Cui; Felix Amevor; Yan Wang; Xiaosong Jiang; Mohan Qiu; Huarui Du; Qing Zhu; Chaowu Yang; Yiping Liu. 2021. "miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken." Genes 12, no. 6: 814.

Journal article
Published: 25 May 2021 in Animals
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Long non-coding RNAs (lncRNAs) and mRNAs are temporally expressed during chicken follicle development. However, follicle transcriptome studies in chickens with timepoints relating to changes in luteinizing hormone (LH) levels are rare. In this study, gene expression in Rohman layers was investigated at three distinct stages of the ovulatory cycle: zeitgeber time 0 (ZT0, 9:00 a.m.), zeitgeber time 12 (ZT12, 9:00 p.m.), and zeitgeber time 20 (ZT20, 5:00 a.m.) representing the early, middle, and LH surge stages, respectively, of the ovulatory cycle. Gene expression profiles were explored during follicle development at ZT0, ZT12, and ZT20 using Ribo-Zero RNA sequencing. The three stages were separated into two major stages, including the pre-LH surge and the LH surge stages. A total of 12,479 mRNAs and 7528 lncRNAs were identified among the three stages, and 4531, 523 differentially expressed genes (DEGs) and 2367, 211 differentially expressed lncRNAs (DELs) were identified in the ZT20 vs. ZT12, and ZT12 vs. ZT0, comparisons. Functional enrichment analysis revealed that genes involved in cell proliferation and metabolism processes (lipid-related) were mainly enriched in the ZT0 and ZT12 stages, respectively, and genes related to oxidative stress, steroids regulation, and inflammatory process were enriched in the ZT20 stage. These findings provide the basis for further investigation of the specific genetic and molecular functions of follicle development in chickens.

ACS Style

Liang Li; Xun Deng; Silu Hu; Zhifu Cui; Zifan Ning; Taotao Gui; Xiaoling Zhao; Diyan Li; Yan Wang; Huadong Yin; Lin Ye; Yaofu Tian; Yao Zhang; Hua Li; Qing Zhu. Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle. Animals 2021, 11, 1533 .

AMA Style

Liang Li, Xun Deng, Silu Hu, Zhifu Cui, Zifan Ning, Taotao Gui, Xiaoling Zhao, Diyan Li, Yan Wang, Huadong Yin, Lin Ye, Yaofu Tian, Yao Zhang, Hua Li, Qing Zhu. Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle. Animals. 2021; 11 (6):1533.

Chicago/Turabian Style

Liang Li; Xun Deng; Silu Hu; Zhifu Cui; Zifan Ning; Taotao Gui; Xiaoling Zhao; Diyan Li; Yan Wang; Huadong Yin; Lin Ye; Yaofu Tian; Yao Zhang; Hua Li; Qing Zhu. 2021. "Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle." Animals 11, no. 6: 1533.

Journal article
Published: 09 December 2020 in Animals
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Several reproductive hormones were reported to be involved in regulating egg yolk precursor synthesis in chickens; however, the mechanism that shows how the liver-blood-ovary signal axis works in relation to age changes has not been reported yet. Therefore, in this study, we observe the morphology and histology of the liver and ovary and determine the serum biochemical parameters and the expression abundance of the critical genes from d90 to 153. Results show that the body weight and liver weight were significantly increased from d132, while the ovary weight increased from d139. Aside from the increase in weight, other distinct changes such as the liver color and an increased deposition of large amounts of yolk precursors into the ovarian follicles were observed. On d139, we observed small fatty vacuoles in the hepatocytes. The results of serum biochemical parameters showed a significant increase in the estradiol (E2) level, first on d125, and then it reached its peak on d132. Meanwhile, the levels of follicle-stimulating hormone (FSH) increased initially and then remained at a high level from d146 to d153, while the levels of luteinizing hormone (LH) increased significantly on d132 and reached the top level on d153. Moreover, the levels of lecithin (LEC), vitellogenin (VTG), very low density lipoprotein y (VLDLy), triglyceride (TG), and total cholesterol (TC) were significantly increased at d125 and were close from d146 to d153. The mRNA and protein expression of estrogen receptor-alpha (ER-α) and E2 levels in the liver and serum, respectively, showed similar changes. Moreover, with reference to an increase in serum E2 level, the mRNA expression of genes related to yolk precursor synthesis (very low density apolipoprotein-II, ApoVLDL-II) and vitellogenin-II (VTG-II), lipogenesis (fatty acid synthase, FAS), and lipid transport (microsomal triglyceride transport protein, MTTP) in the liver showed up-regulation. These results suggest that the correlation between liver-blood-ovary alliances regulate the transport and exchange of synthetic substances to ensure synchronous development and functional coordination between the liver and ovary. We also found that E2 is an activator that is regulated by FSH, which induces histological and functional changes in the hepatocytes through the ER-α pathway.

ACS Style

Zhifu Cui; Felix Kwame Amevor; Qian Feng; Xincheng Kang; Weizhen Song; Qing Zhu; Yan Wang; Diyan Li; Xiaoling Zhao. Sexual Maturity Promotes Yolk Precursor Synthesis and Follicle Development in Hens via Liver-Blood-Ovary Signal Axis. Animals 2020, 10, 2348 .

AMA Style

Zhifu Cui, Felix Kwame Amevor, Qian Feng, Xincheng Kang, Weizhen Song, Qing Zhu, Yan Wang, Diyan Li, Xiaoling Zhao. Sexual Maturity Promotes Yolk Precursor Synthesis and Follicle Development in Hens via Liver-Blood-Ovary Signal Axis. Animals. 2020; 10 (12):2348.

Chicago/Turabian Style

Zhifu Cui; Felix Kwame Amevor; Qian Feng; Xincheng Kang; Weizhen Song; Qing Zhu; Yan Wang; Diyan Li; Xiaoling Zhao. 2020. "Sexual Maturity Promotes Yolk Precursor Synthesis and Follicle Development in Hens via Liver-Blood-Ovary Signal Axis." Animals 10, no. 12: 2348.

Erratum
Published: 07 December 2020 in Animals
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The authors wish to make the following corrections to their paper

ACS Style

Jing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84. Animals 2020, 10, 2322 .

AMA Style

Jing Zhao, Xiaoxu Shen, Xinao Cao, Haorong He, Shunshun Han, Yuqi Chen, Can Cui, Yuanhang Wei, Yan Wang, Diyan Li, Qing Zhu, Huadong Yin. Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84. Animals. 2020; 10 (12):2322.

Chicago/Turabian Style

Jing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. 2020. "Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84." Animals 10, no. 12: 2322.

Journal article
Published: 29 January 2020 in Toxins
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T-2 toxin is type A trichothecenes mycotoxin, which produced by fusarium species in cereal grains. T-2 toxin has been shown to induce a series of toxic effects on the health of human and animal, such as immunosuppression and carcinogenesis. Previous study has proven that T-2 toxin caused hepatotoxicity in chicken, but the regulatory mechanism is unclear. In the present study, we assessed the toxicological effect of T-2 toxin on apoptosis and autophagy in hepatocytes. The total of 120 1-day-old healthy broilers were allocated randomly into four groups and reared for 21 day with complete feed containing 0 mg/kg, 0.5 mg/kg, 1 mg/kg or 2 mg/kg T-2 toxin, respectively. The results showed that the apoptosis rate and pathological changes degree hepatocytes were aggravated with the increase of T-2 toxin. At the molecular mechanism level, T-2 toxin induced mitochondria-mediated apoptosis by producing reactive oxygen species, promoting cytochrome c translocation between the mitochondria and cytoplasm, and thus promoting apoptosomes formation. Meanwhile, the expression of the autophagy-related protein, ATG5, ATG7 and Beclin-1, and the LC3-II/LC3-I ratio were increased, while p62 was downregulated, suggesting T-2 toxin caused autophagy in hepatocytes. Further experiments demonstrated that the PI3K/AKT/mTOR signal may be participated in autophagy induced by T-2 toxin in chicken hepatocytes. These data suggest a possible underlying molecular mechanism for T-2 toxin that induces apoptosis and autophagy in chicken hepatocytes

ACS Style

Huadong Yin; Shunshun Han; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins 2020, 12, 90 .

AMA Style

Huadong Yin, Shunshun Han, Yuqi Chen, Yan Wang, Diyan Li, Qing Zhu. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins. 2020; 12 (2):90.

Chicago/Turabian Style

Huadong Yin; Shunshun Han; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. 2020. "T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes." Toxins 12, no. 2: 90.

Journal article
Published: 23 January 2020 in International Journal of Molecular Sciences
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CSRP3/MLP (cysteine-rich protein 3/muscle Lim protein), a member of the cysteine-rich protein family, is a muscle-specific LIM-only factor specifically expressed in skeletal muscle. CSRP3 is critical in maintaining the structure and function of normal muscle. To investigate the mechanism of disease in CSRP3 myopathy, we performed siRNA-mediated CSRP3 knockdown in chicken primary myoblasts. CSRP3 silencing resulted in the down-regulation of the expression of myogenic genes and the up-regulation of atrophy-related gene expressions. We found that CSRP3 interacted with LC3 protein to promote the formation of autophagosomes during autophagy. CSRP3-silencing impaired myoblast autophagy, as evidenced by inhibited autophagy-related ATG5 and ATG7 mRNA expression levels, and inhibited LC3II and Beclin-1 protein accumulation. In addition, impaired autophagy in CSRP3-silenced cells resulted in increased sensitivity to apoptosis cell death. CSRP3-silenced cells also showed increased caspase-3 and caspase-9 cleavage. Moreover, apoptosis induced by CSRP3 silencing was alleviated after autophagy activation. Together, these results indicate that CSRP3 promotes the correct formation of autophagosomes through its interaction with LC3 protein, which has an important role in skeletal muscle remodeling and maintenance.

ACS Style

Can Cui; Shunshun Han; Shuyue Tang; Haorong He; Xiaoxu Shen; Jing Zhao; Yuqi Chen; Yuanhang Wei; Yan Wang; Qing Zhu; Diyan Li; And Huadong Yin. The Autophagy Regulatory Molecule CSRP3 Interacts with LC3 and Protects Against Muscular Dystrophy. International Journal of Molecular Sciences 2020, 21, 749 .

AMA Style

Can Cui, Shunshun Han, Shuyue Tang, Haorong He, Xiaoxu Shen, Jing Zhao, Yuqi Chen, Yuanhang Wei, Yan Wang, Qing Zhu, Diyan Li, And Huadong Yin. The Autophagy Regulatory Molecule CSRP3 Interacts with LC3 and Protects Against Muscular Dystrophy. International Journal of Molecular Sciences. 2020; 21 (3):749.

Chicago/Turabian Style

Can Cui; Shunshun Han; Shuyue Tang; Haorong He; Xiaoxu Shen; Jing Zhao; Yuqi Chen; Yuanhang Wei; Yan Wang; Qing Zhu; Diyan Li; And Huadong Yin. 2020. "The Autophagy Regulatory Molecule CSRP3 Interacts with LC3 and Protects Against Muscular Dystrophy." International Journal of Molecular Sciences 21, no. 3: 749.

Journal article
Published: 04 January 2020 in Animals
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The development of skeletal muscle satellite cells (SMSCs) is a complex process that could be regulated by many genes. Previous studies have shown that Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation, differentiation, and apoptosis in mouse. However, the function of HDAC4 in chicken muscle development is still unknown. Given that chicken is a very important meat-producing animal that is also an ideal model to study skeletal muscle development, we explored the functions of HDAC4 in chicken SMSCs after the interference of HDAC4. The results showed that HDAC4 was enriched in embryonic skeletal muscle, and it was highly expressed in embryonic muscle than in postnatal muscles. Meanwhile, knockdown of HDAC4 could significantly inhibit the proliferation and differentiation of chicken SMSCs but had no effect on the apoptosis of SMSCs as observed in a series of experiment conducted in vitro. These results indicated that HDAC4 might play a positive role in chicken skeletal muscle growth and development.

ACS Style

Jing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84 .

AMA Style

Jing Zhao, Xiaoxu Shen, Xinao Cao, Haorong He, Shunshun Han, Yuqi Chen, Can Cui, Yuanhang Wei, Yan Wang, Diyan Li, Qing Zhu, Huadong Yin. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals. 2020; 10 (1):84.

Chicago/Turabian Style

Jing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. 2020. "HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells." Animals 10, no. 1: 84.

Research paper
Published: 01 January 2020 in Virulence
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Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and treatment of AL, thus screening of significant immune markers is needed to promote the development of disease-resistant breeds. In this study, data-independent acquisition (DIA) technology was used to detect the DEPs of three breeds of chicken according to different comparison to investigate the potential markers. Results showed special DEPs for spleen development of each breed were detected, such as PCNT, DDB2, and ZNF62. These DEPs were involved in intestinal immune network used in production of IgA signaling pathways and related to immune response which can be used as potential markers for spleen development in different breeds. The DEPs such as RAB44 and TPN involved in viral myocarditis, transcriptional misregulation in cancer, and tuberculosis can be used as potential markers of spleen immune response after ALV-J infection in chickens. Pair-wise analysis was performed for the three breeds after the infection of ALV-J. The proteins such as RFX1, TAF10, and VH1 were differently expressed between three breeds. These DEPs involved in antigen processing and expression, acute myelogenous leukemia, and viral carcinogenesis can be used as potential immune markers after ALV-J infection of different genetic backgrounds. The screening of potential markers at protein level provides a strong theoretical research basis for disease resistance breeding in poultry.

ACS Style

Fei Ye; Yan Wang; Qijian He; Zhaoshuo Wang; Enyue Ma; Shiliang Zhu; Heling Yu; Huadong Yin; Xiaoling Zhao; Diyan Li; Hengyong Xu; Hua Li; Qing Zhu. Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic. Virulence 2020, 11, 1158 -1176.

AMA Style

Fei Ye, Yan Wang, Qijian He, Zhaoshuo Wang, Enyue Ma, Shiliang Zhu, Heling Yu, Huadong Yin, Xiaoling Zhao, Diyan Li, Hengyong Xu, Hua Li, Qing Zhu. Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic. Virulence. 2020; 11 (1):1158-1176.

Chicago/Turabian Style

Fei Ye; Yan Wang; Qijian He; Zhaoshuo Wang; Enyue Ma; Shiliang Zhu; Heling Yu; Huadong Yin; Xiaoling Zhao; Diyan Li; Hengyong Xu; Hua Li; Qing Zhu. 2020. "Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic." Virulence 11, no. 1: 1158-1176.

Journal article
Published: 30 December 2019 in Animals
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Due to the increasing demand for producing chickens with high meat quality, there is a need to determine its mode of action on chicken meat quality traits across a wider age spectrum. In this study, five groups of 200 male Da-Heng meat type birds were reared until slaughter age of 60, 90, 120, 150, 180 days old and breast muscle performance, meat quality traits, and myofiber characteristics were evaluated. The larger body weight and breast weight of chicken are based on larger myofiber diameter and area, less myofiber density for the older birds than younger birds. There was an age effect on all meat quality traits of chicken breast muscle (p < 0.05). Older chickens often presented a higher pH, lower drip loss, higher shear force, darker, and redder breast meat. The correlation coefficients showed that myofiber characteristics played an important role in breast pH values, drip loss, and meat color (p < 0.05). Besides, significant correlations were also found between meat quality traits (p < 0.05). Further studies are needed to explore the biochemical character and potential molecular mechanism of chicken breast muscle to determine the factors that causes these age-related differences in meat quality in the current study.

ACS Style

Jingjing Li; Chaowu Yang; Han Peng; Huadong Yin; Yan Wang; Yaodong Hu; Chunlin Yu; Xiaosong Jiang; Huarui Du; Qingyun Li; Yiping Liu; Li. Effects of Slaughter Age on Muscle Characteristics and Meat Quality Traits of Da-Heng Meat Type Birds. Animals 2019, 10, 69 .

AMA Style

Jingjing Li, Chaowu Yang, Han Peng, Huadong Yin, Yan Wang, Yaodong Hu, Chunlin Yu, Xiaosong Jiang, Huarui Du, Qingyun Li, Yiping Liu, Li. Effects of Slaughter Age on Muscle Characteristics and Meat Quality Traits of Da-Heng Meat Type Birds. Animals. 2019; 10 (1):69.

Chicago/Turabian Style

Jingjing Li; Chaowu Yang; Han Peng; Huadong Yin; Yan Wang; Yaodong Hu; Chunlin Yu; Xiaosong Jiang; Huarui Du; Qingyun Li; Yiping Liu; Li. 2019. "Effects of Slaughter Age on Muscle Characteristics and Meat Quality Traits of Da-Heng Meat Type Birds." Animals 10, no. 1: 69.

Journal article
Published: 04 December 2019 in Animals
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In this study, using a laying hen model, we determined the expression of FOXL2 and RSPO1 in different central and peripheral tissue and ovarian follicles at different stages of development. At the same time, mRNA expression of both genes in granulosa and theca cells harvested from follicles at different stages of folliculogenesis was also evaluated. Finally, we assessed the effect of leptin treatment on expression of FOXL2 and RSPO1 in in vitro cultured granulosa cells harvested from 1–5 mm to F3–F1 follicles. Our RT-qPCR results revealed that a comparatively higher expression of FOXL2 and RSPO1 was observed in ovary, hypothalamus, and pituitary. Abundant mRNA expression of FOXL2 was observed in small prehierarchical follicles (1–1.9 and 2–2.9 mm follicles; p < 0.05), whereas mRNA expression of RSPO1 showed an increasing trend in large hierarchical follicles (F5–F1), and its abundant expression was observed in post-ovulatory follicles. FOXL2 mRNA expression was stable in granulosa cells harvested from 3–5 mm to F4 follicles, and exhibited a significantly higher expression in large hierarchical follicles. Conversely, relatively low mRNA expression of FOXL2 was observed in theca cells. RSPO1 mRNA expression was relatively lower in granulosa cells; however, theca cells exhibited a significantly higher mRNA expression of RSPO1 in F4 to F1 follicles. In the next experiment, we treated the in vitro cultured granulosa cells with different concentrations (1, 10, 100, and 1000 ng/mL) of exogenous leptin. Compared to the control group, a significant increase in the expression of FOXL2 was observed in groups treated with 1, 10, and 100 ng/mL leptin, whereas expression of RSPO1 was increased in all leptin-treated groups. When treated with 100 ng/mL leptin, FOXL2 and RSPO1 expression was upregulated in cultured granulosa cells harvested from both large hierarchical (F3–F1) and small prehierarchical follicles (1–5 mm). Based on these findings and evidence from mainstream literature, we envisage that FOXL2 and RSPO1 genes (in connection with hypothalamic-hypophysis axis) and leptin (via modulation of FOXL2 and RSPO1 expression) might have significant physiological roles, at least in part, in modulating the ovarian mechanisms, such as follicle development, selection, and steroidogenesis in laying hens.

ACS Style

Weihe Niu; Izhar Hyder Qazi; Sichen Li; Xiaoling Zhao; Huadong Yin; Yan Wang; Qing Zhu; Hongbing Han; Guangbin Zhou; Xiaohui Du. Expression of FOXL2 and RSPO1 in Hen Ovarian Follicles and Implication of Exogenous Leptin in Modulating Their mRNA Expression in In Vitro Cultured Granulosa Cells. Animals 2019, 9, 1083 .

AMA Style

Weihe Niu, Izhar Hyder Qazi, Sichen Li, Xiaoling Zhao, Huadong Yin, Yan Wang, Qing Zhu, Hongbing Han, Guangbin Zhou, Xiaohui Du. Expression of FOXL2 and RSPO1 in Hen Ovarian Follicles and Implication of Exogenous Leptin in Modulating Their mRNA Expression in In Vitro Cultured Granulosa Cells. Animals. 2019; 9 (12):1083.

Chicago/Turabian Style

Weihe Niu; Izhar Hyder Qazi; Sichen Li; Xiaoling Zhao; Huadong Yin; Yan Wang; Qing Zhu; Hongbing Han; Guangbin Zhou; Xiaohui Du. 2019. "Expression of FOXL2 and RSPO1 in Hen Ovarian Follicles and Implication of Exogenous Leptin in Modulating Their mRNA Expression in In Vitro Cultured Granulosa Cells." Animals 9, no. 12: 1083.

Research article
Published: 25 November 2019 in BioMed Research International
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T-2 toxin is a trichothecene mycotoxin produced by fungi which are known to contaminate cereals, especially in wheat and corn. T-2 toxin is known to cause a range of toxic effects in humans and animals, including immunosuppression and carcinogenesis. Although the effects of T-2 toxin on condition of chickens’ spleens have been reported, there has been no systematic study of damage to the spleen of broiler chickens exposed to T-2 toxin. The purpose of the present study was to assess the effects of T-2 toxin on pathology, rates of apoptosis, oxidative stress, and T-lymphocyte subsets in the spleen of broiler chickens. One hundred and twenty male broiler chickens were randomly assigned to one of four groups (30 birds per group), fed 0 mg/kg (control), 0.5 mg/kg, 1 mg/kg, or 2 mg/kg T-2 toxin, respectively. After 21 days, chickens exposed to T-2 toxin demonstrated decreased relative weight and size of the spleen, increased percentage of apoptotic splenocytes, and evident lesions. Concentrations of reactive oxygen species and MDA content increased in splenocytes during T-2 toxin treatments, whereas activities of SOD, CAT, and GSH-PX decreased. The ratio of CD4+/CD8+ T cells also decreased as the dose of T-2 toxin increased. Overall, these results suggest that T-2 toxin causes oxidative stress, leading to increased rates of splenocyte apoptosis and might impair the splenic immune function of broiler chickens.

ACS Style

Yuqi Chen; Shunshun Han; Yan Wang; Diyan Li; Xiaoling Zhao; Qing Zhu; Huadong Yin. Oxidative Stress and Apoptotic Changes in Broiler Chicken Splenocytes Exposed to T-2 Toxin. BioMed Research International 2019, 2019, 1 -9.

AMA Style

Yuqi Chen, Shunshun Han, Yan Wang, Diyan Li, Xiaoling Zhao, Qing Zhu, Huadong Yin. Oxidative Stress and Apoptotic Changes in Broiler Chicken Splenocytes Exposed to T-2 Toxin. BioMed Research International. 2019; 2019 ():1-9.

Chicago/Turabian Style

Yuqi Chen; Shunshun Han; Yan Wang; Diyan Li; Xiaoling Zhao; Qing Zhu; Huadong Yin. 2019. "Oxidative Stress and Apoptotic Changes in Broiler Chicken Splenocytes Exposed to T-2 Toxin." BioMed Research International 2019, no. : 1-9.

Research article
Published: 31 October 2019 in BioMed Research International
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Bitterness is an important taste sensation for chickens, which provides useful sensory information for acquisition and selection of diet, and warns them against ingestion of potentially harmful and noxious substances in nature. Bitter taste receptors (T2Rs) mediate the recognition of bitter compounds belonging to a family of proteins known as G-protein coupled receptors. The aim of this study was to identify and evaluate the expression of T2R7 in chicken tongue tissue and construct cT2R7-1 and cT2R7-2-expressing HEK-293T cells to access the expression of PLCβ2 and ITPR3 after exposure with different concentrations of the bitter compounds. Using real-time PCR, we show that the relative expression level of T2R7 mRNA in 5, 1, 0.1, and 10−3 mM of camphor and erythromycin solutions and 5 mM of chlorpheniramine maleate solutions was significantly higher than that in 50 mM KCL solutions. We confirmed that the bitter taste receptor T2R7 and downstream signaling effectors are sensitive to different concentrations of bitter compounds. Moreover, T2R7-1 (corresponding to the unique haplotype of the Tibetan chicken) had higher sensitivity to bitter compounds compared with that of T2R7-2 (corresponding to the unique haplotype of the Jiuyuan black-chicken). These results provide great significance of taste response on dietary intake to improve chicken feeding efficiency in poultry production and have certain reference value for future taste research in other bird species.

ACS Style

Yuan Su; Hang Jie; Qing Zhu; Xiaoling Zhao; Yan Wang; Huadong Yin; Shailendra Mishra; Diyan Li. Effect of Bitter Compounds on the Expression of Bitter Taste Receptor T2R7 Downstream Signaling Effectors in cT2R7/pDisplay-Gα16/gust44/pcDNA3.1 (+) Cells. BioMed Research International 2019, 2019, 1 -12.

AMA Style

Yuan Su, Hang Jie, Qing Zhu, Xiaoling Zhao, Yan Wang, Huadong Yin, Shailendra Mishra, Diyan Li. Effect of Bitter Compounds on the Expression of Bitter Taste Receptor T2R7 Downstream Signaling Effectors in cT2R7/pDisplay-Gα16/gust44/pcDNA3.1 (+) Cells. BioMed Research International. 2019; 2019 ():1-12.

Chicago/Turabian Style

Yuan Su; Hang Jie; Qing Zhu; Xiaoling Zhao; Yan Wang; Huadong Yin; Shailendra Mishra; Diyan Li. 2019. "Effect of Bitter Compounds on the Expression of Bitter Taste Receptor T2R7 Downstream Signaling Effectors in cT2R7/pDisplay-Gα16/gust44/pcDNA3.1 (+) Cells." BioMed Research International 2019, no. : 1-12.

Journal article
Published: 16 October 2019 in International Journal of Molecular Sciences
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Myoferlin (MyoF), which is a calcium/phospholipid-binding protein expressed in cardiac and muscle tissues, belongs to the ferlin family. While MyoF promotes myoblast differentiation, the underlying mechanisms remain poorly understood. Here, we found that MyoF not only promotes C2C12 myoblast differentiation, but also inhibits muscle atrophy and autophagy. In the present study, we found that myoblasts fail to develop into mature myotubes due to defective differentiation in the absence of MyoF. Meanwhile, MyoF regulates the expression of atrophy-related genes (Atrogin-1 and MuRF1) to rescue muscle atrophy. Furthermore, MyoF interacts with Dishevelled-2 (Dvl-2) to activate canonical Wnt signaling. MyoF facilitates Dvl-2 ubiquitination resistance by reducing LC3-labeled Dvl-2 levels and antagonizing the autophagy system. In conclusion, we found that MyoF plays an important role in myoblast differentiation during skeletal muscle atrophy. At the molecular level, MyoF protects Dvl-2 against autophagy-mediated degradation, thus promoting activation of the Wnt/β-catenin signaling pathway. Together, our findings suggest that MyoF, through stabilizing Dvl-2 and preventing autophagy, regulates Wnt/β-catenin signaling-mediated skeletal muscle development.

ACS Style

Shunshun Han; Can Cui; Haorong He; Xiaoxu Shen; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. Myoferlin Regulates Wnt/β-Catenin Signaling-Mediated Skeletal Muscle Development by Stabilizing Dishevelled-2 Against Autophagy. International Journal of Molecular Sciences 2019, 20, 5130 .

AMA Style

Shunshun Han, Can Cui, Haorong He, Xiaoxu Shen, Yuqi Chen, Yan Wang, Diyan Li, Qing Zhu, Huadong Yin. Myoferlin Regulates Wnt/β-Catenin Signaling-Mediated Skeletal Muscle Development by Stabilizing Dishevelled-2 Against Autophagy. International Journal of Molecular Sciences. 2019; 20 (20):5130.

Chicago/Turabian Style

Shunshun Han; Can Cui; Haorong He; Xiaoxu Shen; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. 2019. "Myoferlin Regulates Wnt/β-Catenin Signaling-Mediated Skeletal Muscle Development by Stabilizing Dishevelled-2 Against Autophagy." International Journal of Molecular Sciences 20, no. 20: 5130.

Research article
Published: 08 August 2019 in BioMed Research International
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Inhibin α (INHA) is a candidate gene controlling ovulation in poultry. As the functional center of inhibin, INHA is a molecular marker associated with egg-laying performance. The objective of the current study was to analyze the expression differences of INHA in reproductive system and single nucleotide polymorphisms (SNPs) associations with reproductive traits in chickens. A total of 260 LuHua chickens (barred-feather chicken) were adopted. Twelve SNPs were detected in INHA gene. Among the exonic SNPs, three (g. 22177991A>G, g. 22178249G>C, and g. 22178414G>A) were missense mutations, resulting in the amino acid substitutions Val→Ala, Ala→Gly, and Ala→Gly, respectively. Four SNPs in the 3 untranslated region of INHA were predicted to either disturb or create microRNA-target interactions. Five SNPs (g. 22176870T>C, g. 22177100T>C, g. 22177149T>C, g. 22177991A>G, and g. 22178975G>A) were significantly associated with the number of eggs at 300 d of age (EN) (P < 0.05). Birds carrying GA genotype exhibited more EN than those with AA genotype (P < 0.01). In addition, quantitative real-time PCR revealed that INHA is mainly expressed in follicles on d 300 in chickens. Firstly, INHA expression increased and then decreased. The highest INHA mRNA abundance was found in the fifth largest preovulatory follicle (F5) (P < 0.01). In the prehierarchical follicles, INHA mRNA expression increased dramatically in small yellow follicles (SYF) (P < 0.01). Western blotting analysis showed that the INHA protein expression profile in the follicle was similar to its mRNA counterpart with greater expression in F5 and SYF follicles and lowest expression in F1 follicles (P < 0.05). These results suggest that INHA is a potential candidate gene improving reproductive traits in chickens.

ACS Style

Zhifu Cui; Lingbin Liu; Xiaoling Zhao; Jinshan Ran; Yan Wang; Huadong Yin; Diyan Li; Qing Zhu. Analysis of Expression and Single Nucleotide Polymorphisms of INHA Gene Associated with Reproductive Traits in Chickens. BioMed Research International 2019, 2019, 1 -11.

AMA Style

Zhifu Cui, Lingbin Liu, Xiaoling Zhao, Jinshan Ran, Yan Wang, Huadong Yin, Diyan Li, Qing Zhu. Analysis of Expression and Single Nucleotide Polymorphisms of INHA Gene Associated with Reproductive Traits in Chickens. BioMed Research International. 2019; 2019 ():1-11.

Chicago/Turabian Style

Zhifu Cui; Lingbin Liu; Xiaoling Zhao; Jinshan Ran; Yan Wang; Huadong Yin; Diyan Li; Qing Zhu. 2019. "Analysis of Expression and Single Nucleotide Polymorphisms of INHA Gene Associated with Reproductive Traits in Chickens." BioMed Research International 2019, no. : 1-11.

Research article
Published: 11 July 2019 in BioMed Research International
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FOXO3, which encodes the transcription factor forkhead box O-3 (FoxO3), is a member of the FOXO subfamily of the forkhead box (FOX) family. FOXO3 can be negatively regulated by its phosphorylation by the PI3K/Akt signaling pathway and ultimately drives apoptosis when activated. In mammalian ovaries, the FOXO3 protein regulates atresia and follicle growth by promoting apoptosis of ovarian granulosa cells. Nonetheless, the specific effects of the FOXO3 protein on granulosa apoptosis of avian ovaries have not been elucidated. Therefore, we studied FOXO3 expression in follicles with different organization and at all hierarchical levels of chicken follicles. Via an immunofluorescence assay, the chicken follicular theca at all hierarchical levels were found to be strongly stained with an anti-FOXO3 antibody. In chicken primary ovarian granulosa cells, mRNA levels of proapoptotic factors BNIP3 and BCL2L11 decreased in the absence of FOXO3, and so did PARP-1 and cleaved caspase 3 protein levels. After treatment with a recombinant FOXO3 protein, PARP-1 and caspase 3 protein levels increased, along with mRNA levels of Bnip3 and BCL2L11 (significantly, p<0.05). In addition, FOXO3 was downregulated in chicken granulosa cells when different estradiol or FSH concentrations were applied. In conclusion, FOXO3 is expressed in chicken reproductive tissues, including follicles and ovarian granulosa cells, and promotes apoptosis of chicken ovarian granulosa cells.

ACS Style

Can Cui; Shunshun Han; Huadong Yin; Bin Luo; Xiaoxu Shen; Fuling Yang; Zihao Liu; Qing Zhu; Diyan Li; Yan Wang. FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens. BioMed Research International 2019, 2019, 1 -9.

AMA Style

Can Cui, Shunshun Han, Huadong Yin, Bin Luo, Xiaoxu Shen, Fuling Yang, Zihao Liu, Qing Zhu, Diyan Li, Yan Wang. FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens. BioMed Research International. 2019; 2019 ():1-9.

Chicago/Turabian Style

Can Cui; Shunshun Han; Huadong Yin; Bin Luo; Xiaoxu Shen; Fuling Yang; Zihao Liu; Qing Zhu; Diyan Li; Yan Wang. 2019. "FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens." BioMed Research International 2019, no. : 1-9.

Journal article
Published: 02 April 2019 in BMC Genomics
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There are hundreds of phenotypically distinguishable domestic chicken breeds or lines with highly specialized traits worldwide, which provide a unique opportunity to illustrate how selection shapes patterns of genetic variation. There are many local chicken breeds in China. Here, we provide a population genome landscape of genetic variations in 86 domestic chickens representing 10 phenotypically diverse breeds. Genome-wide analysis indicated that sex chromosomes have less genetic diversity and are under stronger selection than autosomes during domestication and local adaptation. We found an evidence of admixture between Tibetan chickens and other domestic population. We further identified strong signatures of selection affecting genomic regions that harbor genes underlying economic traits (typically related to feathers, skin color, growth, reproduction and aggressiveness) and local adaptation (to high altitude). By comparing the genomes of the Tibetan and lowland fowls, we identified genes associated with high-altitude adaptation in Tibetan chickens were mainly involved in energy metabolism, body size maintenance and available food sources. The work provides crucial insights into the distinct evolutionary scenarios occurring under artificial selection for agricultural production and under natural selection for success at high altitudes in chicken. Several genes were identified as candidates for chicken economic traits and other phenotypic traits.

ACS Style

Diyan Li; Yan Li; Miao Li; Tiandong Che; Shilin Tian; Binlong Chen; Xuming Zhou; Guolong Zhang; Uma Gaur; Majing Luo; Kai Tian; Mengnan He; Shen He; Zhongxian Xu; Long Jin; Qianzi Tang; Yifei Dai; Huailiang Xu; Yaodong Hu; Xiaoling Zhao; Huadong Yin; Yan Wang; Rongjia Zhou; Chaowu Yang; Huarui Du; Xiaosong Jiang; Qing Zhu; Mingzhou Li. Population genomics identifies patterns of genetic diversity and selection in chicken. BMC Genomics 2019, 20, 1 -12.

AMA Style

Diyan Li, Yan Li, Miao Li, Tiandong Che, Shilin Tian, Binlong Chen, Xuming Zhou, Guolong Zhang, Uma Gaur, Majing Luo, Kai Tian, Mengnan He, Shen He, Zhongxian Xu, Long Jin, Qianzi Tang, Yifei Dai, Huailiang Xu, Yaodong Hu, Xiaoling Zhao, Huadong Yin, Yan Wang, Rongjia Zhou, Chaowu Yang, Huarui Du, Xiaosong Jiang, Qing Zhu, Mingzhou Li. Population genomics identifies patterns of genetic diversity and selection in chicken. BMC Genomics. 2019; 20 (1):1-12.

Chicago/Turabian Style

Diyan Li; Yan Li; Miao Li; Tiandong Che; Shilin Tian; Binlong Chen; Xuming Zhou; Guolong Zhang; Uma Gaur; Majing Luo; Kai Tian; Mengnan He; Shen He; Zhongxian Xu; Long Jin; Qianzi Tang; Yifei Dai; Huailiang Xu; Yaodong Hu; Xiaoling Zhao; Huadong Yin; Yan Wang; Rongjia Zhou; Chaowu Yang; Huarui Du; Xiaosong Jiang; Qing Zhu; Mingzhou Li. 2019. "Population genomics identifies patterns of genetic diversity and selection in chicken." BMC Genomics 20, no. 1: 1-12.

Journal article
Published: 01 January 2019 in Brazilian Journal of Poultry Science
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ACS Style

F Yang; L He; B Luo; F Ye; C Cui; X Yu; H Xu; X Zhao; H Yin; D Li; Q Zhu; Y Wang. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science 2019, 21, 1 .

AMA Style

F Yang, L He, B Luo, F Ye, C Cui, X Yu, H Xu, X Zhao, H Yin, D Li, Q Zhu, Y Wang. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science. 2019; 21 (2):1.

Chicago/Turabian Style

F Yang; L He; B Luo; F Ye; C Cui; X Yu; H Xu; X Zhao; H Yin; D Li; Q Zhu; Y Wang. 2019. "Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis." Brazilian Journal of Poultry Science 21, no. 2: 1.

Original article
Published: 01 January 2019 in Brazilian Journal of Poultry Science
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The melanocortin 1 receptor (MC1R) gene plays a key role in controlling the deposition of melanin. In mammals, the MC1Rgene is regarded as a major candidate gene in the control of melanin formation. In domestic animals, the MC1R gene mainly controls the expression of coat, skin, and plumage color in mammals and birds. In order to breed chickens with dark-green shank faster, we screened the molecular markers for shank color in a HS chicken population by exploring the relationship between polymorphism of the MC1R gene and three different shank colors (light green, dark green and yellow). Two primer pairs for code region of the MC1R gene were designed in the basic of chicken genomic sequence. DNA sequencing was performed to detect the polymorphisms and PCR was used to amplify DNA fragment. Sequences analysis indicated that 7 SNPs were predominant the three HS chicken populations with different shank color, including g.18,287,945C>T, g.18,288,088T>C, g.18,288,150G>A, g.18,288,303A>G, g.18,288,512G>A, g.18,288,513T>C, and g.18,288,520A>C. Association analysis revealed that the dark-green shank population showed moderate polymorphism, whereas the light-green shank population showed low polymorphism among overall 7 SNPs and that SNP6 (g.18,288,513T>C) may be significantly associated with three different shank colors in HS chickens. The haplotype CTGGACA had the largest haplotype frequencies, accounting for 56.22%, and the haplotype combination H1H1 is mainly distributed in the dark-green shank population, and may be used as molecular maker for marker-assisted selection of shank color in HS chickens.

ACS Style

X Shen; Y Wang; C Cui; X Zhao; D Li; Q Zhu; X Jiang; C Yang; M Qiu; C Yu; Q Li; H Du; Z Zhang; H Yin. Detection of Snps in the Melanocortin 1-Receptor (MC1R) and Its Association with Shank Color Trait in Hs Chicken. Brazilian Journal of Poultry Science 2019, 21, 1 .

AMA Style

X Shen, Y Wang, C Cui, X Zhao, D Li, Q Zhu, X Jiang, C Yang, M Qiu, C Yu, Q Li, H Du, Z Zhang, H Yin. Detection of Snps in the Melanocortin 1-Receptor (MC1R) and Its Association with Shank Color Trait in Hs Chicken. Brazilian Journal of Poultry Science. 2019; 21 (3):1.

Chicago/Turabian Style

X Shen; Y Wang; C Cui; X Zhao; D Li; Q Zhu; X Jiang; C Yang; M Qiu; C Yu; Q Li; H Du; Z Zhang; H Yin. 2019. "Detection of Snps in the Melanocortin 1-Receptor (MC1R) and Its Association with Shank Color Trait in Hs Chicken." Brazilian Journal of Poultry Science 21, no. 3: 1.

Original article
Published: 01 January 2019 in Brazilian Journal of Poultry Science
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Chinese indigenous chicken breeds are geographically widespread, and a total of 116 indigenous chicken breeds are listed as Chinese national genetic resources. However, these indigenous chicken breeds are facing serious challenges as declining population and germplasm degeneration because lots of commercial chicken breeds had been introduced. In this study, the genetic variations of eleven Chinese indigenous chicken breeds of Sichuan province and three commercial chicken breeds were investigated based on the partial mitochondrial DNA D-loop of 487bp in length. 147 individuals from 14 breeds were examined and 34 haplotypes were observed. Genetic diversity analysis showed that the highest haplotype diversity level was found in Dahen Chicken (DH) population, while the Arbor Acres Chicken (WF) and Roman layer (RM) showed lower genetic diversity levels. The long-term artificial selection may lead to reduced nucleotide diversity. Genetic population differentiation analysis indicated that most of the variation (80.80%) was attributed to variations among breeds. Phylogenetic analysis revealed that these individuals were divided into four distinct genetic clades, including cluster A, B, C and D. Eighteen haplotypes were classified as cluster A, eight haplotypes were classified as cluster B, five haplotypes were classified as cluster C and three haplotypes were classified as cluster D. There was no breed-specific clade. Our study firstly identified the population’s genetic structure of Chinese indigenous chickens and the most important commercial breeds in Sichuan province, though the genetic diversity of indigenous breeds did not suffer obvious decrease, but could be helpful for efficient artificial breeding selection and genetic resources conservation.

ACS Style

C Yu; M Qiu; X Jiang; Z Zhang; H Du; Q Li; B Xia; X Song; C Hu; X Xiong; L Yang; H Peng; J Chen; Y Wang; C Yang. Genetic Diversity and Phyletic Evolution of Eleven Chinese Indigenous and Three Commercial Chicken Breeds by mtDNA Sequences. Brazilian Journal of Poultry Science 2019, 21, 1 .

AMA Style

C Yu, M Qiu, X Jiang, Z Zhang, H Du, Q Li, B Xia, X Song, C Hu, X Xiong, L Yang, H Peng, J Chen, Y Wang, C Yang. Genetic Diversity and Phyletic Evolution of Eleven Chinese Indigenous and Three Commercial Chicken Breeds by mtDNA Sequences. Brazilian Journal of Poultry Science. 2019; 21 (4):1.

Chicago/Turabian Style

C Yu; M Qiu; X Jiang; Z Zhang; H Du; Q Li; B Xia; X Song; C Hu; X Xiong; L Yang; H Peng; J Chen; Y Wang; C Yang. 2019. "Genetic Diversity and Phyletic Evolution of Eleven Chinese Indigenous and Three Commercial Chicken Breeds by mtDNA Sequences." Brazilian Journal of Poultry Science 21, no. 4: 1.