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Skeletal muscle plays important roles in animal locomotion, metabolism, and meat production in farm animals. Current studies showed that non-coding RNAs, especially the circular RNA (circRNA) play an indispensable role in skeletal muscle development. Our previous study revealed that several differentially expressed circRNAs among fast muscle growing broilers (FMGB) and slow muscle growing layers (SMGL) may regulate muscle development in the chicken. In this study, a novel differentially expressed circPPP1R13B was identified. Molecular mechanism analysis indicated that circPPP1R13B targets miR-9-5p and negatively regulates the expression of miR-9-5p, which was previously reported to be an inhibitor of skeletal muscle development. In addition, circPPP1R13B positively regulated the expression of miR-9-5p target gene insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) and further activated the downstream insulin like growth factors (IGF)/phosphatidylinositol 3-kinase (PI3K)/AKT serine/threonine kinase (AKT) signaling pathway. The results also showed that the knockdown of circPPP1R13B inhibits chicken skeletal muscle satellite cells (SMSCs) proliferation and differentiation, and the overexpression of circPPP1R13B promotes the proliferation and differentiation of chicken SMSCs. Furthermore, the overexpression of circPPP1R13B could block the inhibitory effect of miR-9-5p on chicken SMSC proliferation and differentiation. In summary, our results suggested that circPPP1R13B promotes chicken SMSC proliferation and differentiation by targeting miR-9-5p and activating IGF/PI3K/AKT signaling pathway.
Xiaoxu Shen; Yuanhang Wei; Guishuang You; Wei Liu; Felix Kwame Amevor; Yao Zhang; Haorong He; Menggen Ma; Yun Zhang; Diyan Li; Qing Zhu; Huadong Yin. Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p. Animals 2021, 11, 2396 .
AMA StyleXiaoxu Shen, Yuanhang Wei, Guishuang You, Wei Liu, Felix Kwame Amevor, Yao Zhang, Haorong He, Menggen Ma, Yun Zhang, Diyan Li, Qing Zhu, Huadong Yin. Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p. Animals. 2021; 11 (8):2396.
Chicago/Turabian StyleXiaoxu Shen; Yuanhang Wei; Guishuang You; Wei Liu; Felix Kwame Amevor; Yao Zhang; Haorong He; Menggen Ma; Yun Zhang; Diyan Li; Qing Zhu; Huadong Yin. 2021. "Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p." Animals 11, no. 8: 2396.
Hollow peanut-shaped NiFe2O4/CoFe2O4 twinned nano-spherical shell composite materials have interconnected electron channels and excellent electrochemical performance, which prompted the use of this unique spatial structure to fabricate efficient electrochemical sensors. In this work, N-doped carbon dots (NCDs) incorporated into magnetic NiFe2O4/CoFe2O4 nanoparticle shell (NiFe2O4/CoFe2O4/NCDs) modified glassy carbon electrode (GCE) was applied to construct a dual-template molecularly imprinted polymer (MIP) based electrochemistry sensor (NiFe2O4/CoFe2O4/NCDs/MIP/GCE) for the simultaneous detection of catechin (CA) and theophylline (TPH). MIP was fabricated by an in-situ electrochemical polymerization strategy based on the theoretical exploration and density functional theory (DFT) computer directional simulation to screen out the optimal functional monomer (L-arginine) and the optimal ratio between the dual template molecules (CA and TPH) and functional monomer. The materials were characterized by SEM, TEM, XRD, XPS, and TGA. Besides, electron binding energy, binding constant, and imprinting factor were investigated. With the optimal conditions, the proposed electrochemical dual detection system showed outstanding analytical performance for the simultaneous sensing of CA and TPH, with an ultralow detection limit (LOD, S/N = 3) of 1.3 nM for CA in 0.01–1 μM (R2 = 0.9956) and 1–50 μM (R2 = 0.9928), as well as a LOD of 20.0 nM for TPH in the linear range of 0.1–100 μM (R2 = 0.9939), respectively. Also, the selectivity and anti-interference performances of the fabricated sensor were performed by differential pulse voltammetry and chronoamperometry, and successfully detected the analyte from tea drinks and human urine samples with the recovery rates ranging from 98.22% to 104.76% and relative standard deviations (RSD) were 1.19%–3.81%, demonstrated the sensor has excellent stability, repeatability, and reproducibility, which paves the way for other platforms to use this nanomaterial for the detection of antioxidant in the filed food safety.
Zhiwei Lu; Xin Du; Mengmeng Sun; Yan Zhang; Yifan Li; Xianxiang Wang; Yanying Wang; Haijun Du; Huadong Yin; Hanbing Rao. Novel dual-template molecular imprinted electrochemical sensor for simultaneous detection of CA and TPH based on peanut twin-like NiFe2O4/CoFe2O4/NCDs nanospheres: Fabrication, application and DFT theoretical study. Biosensors and Bioelectronics 2021, 190, 113408 .
AMA StyleZhiwei Lu, Xin Du, Mengmeng Sun, Yan Zhang, Yifan Li, Xianxiang Wang, Yanying Wang, Haijun Du, Huadong Yin, Hanbing Rao. Novel dual-template molecular imprinted electrochemical sensor for simultaneous detection of CA and TPH based on peanut twin-like NiFe2O4/CoFe2O4/NCDs nanospheres: Fabrication, application and DFT theoretical study. Biosensors and Bioelectronics. 2021; 190 ():113408.
Chicago/Turabian StyleZhiwei Lu; Xin Du; Mengmeng Sun; Yan Zhang; Yifan Li; Xianxiang Wang; Yanying Wang; Haijun Du; Huadong Yin; Hanbing Rao. 2021. "Novel dual-template molecular imprinted electrochemical sensor for simultaneous detection of CA and TPH based on peanut twin-like NiFe2O4/CoFe2O4/NCDs nanospheres: Fabrication, application and DFT theoretical study." Biosensors and Bioelectronics 190, no. : 113408.
Long non-coding RNAs (lncRNAs) and mRNAs are temporally expressed during chicken follicle development. However, follicle transcriptome studies in chickens with timepoints relating to changes in luteinizing hormone (LH) levels are rare. In this study, gene expression in Rohman layers was investigated at three distinct stages of the ovulatory cycle: zeitgeber time 0 (ZT0, 9:00 a.m.), zeitgeber time 12 (ZT12, 9:00 p.m.), and zeitgeber time 20 (ZT20, 5:00 a.m.) representing the early, middle, and LH surge stages, respectively, of the ovulatory cycle. Gene expression profiles were explored during follicle development at ZT0, ZT12, and ZT20 using Ribo-Zero RNA sequencing. The three stages were separated into two major stages, including the pre-LH surge and the LH surge stages. A total of 12,479 mRNAs and 7528 lncRNAs were identified among the three stages, and 4531, 523 differentially expressed genes (DEGs) and 2367, 211 differentially expressed lncRNAs (DELs) were identified in the ZT20 vs. ZT12, and ZT12 vs. ZT0, comparisons. Functional enrichment analysis revealed that genes involved in cell proliferation and metabolism processes (lipid-related) were mainly enriched in the ZT0 and ZT12 stages, respectively, and genes related to oxidative stress, steroids regulation, and inflammatory process were enriched in the ZT20 stage. These findings provide the basis for further investigation of the specific genetic and molecular functions of follicle development in chickens.
Liang Li; Xun Deng; Silu Hu; Zhifu Cui; Zifan Ning; Taotao Gui; Xiaoling Zhao; Diyan Li; Yan Wang; Huadong Yin; Lin Ye; Yaofu Tian; Yao Zhang; Hua Li; Qing Zhu. Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle. Animals 2021, 11, 1533 .
AMA StyleLiang Li, Xun Deng, Silu Hu, Zhifu Cui, Zifan Ning, Taotao Gui, Xiaoling Zhao, Diyan Li, Yan Wang, Huadong Yin, Lin Ye, Yaofu Tian, Yao Zhang, Hua Li, Qing Zhu. Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle. Animals. 2021; 11 (6):1533.
Chicago/Turabian StyleLiang Li; Xun Deng; Silu Hu; Zhifu Cui; Zifan Ning; Taotao Gui; Xiaoling Zhao; Diyan Li; Yan Wang; Huadong Yin; Lin Ye; Yaofu Tian; Yao Zhang; Hua Li; Qing Zhu. 2021. "Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle." Animals 11, no. 6: 1533.
Skeletal muscle satellite cell growth and development is a complicated process driven by multiple genes. The PDZ and LIM domain 5 (PDLIM5) gene has been proven to function in C2C12 myoblast differentiation and is involved in the regulation of skeletal muscle development. The role of PDLIM5 in chicken skeletal muscle satellite cells, however, is unclear. In this study, in order to determine whether the PDLIM5 gene has a function in chicken skeletal muscle satellite cells, we examined the changes in proliferation and differentiation of chicken skeletal muscle satellite cells (SMSCs) after interfering and overexpressing PDLIM5 in cells. In addition, the molecular pathways of the PDLIM5 gene regulating SMSC proliferation and differentiation were analyzed by transcriptome sequencing. Our results show that PDLIM5 can promote the proliferation and differentiation of SMSCs; furthermore, through transcriptome sequencing, it can be found that the differential genes are enriched in the MAPK signaling pathway after knocking down PDLIM5. Finally, it was verified that PDLIM5 played an active role in the proliferation and differentiation of chicken SMSCs by activating the p38-MAPK signaling pathway. These results indicate that PDLIM5 may be involved in the growth and development of chicken skeletal muscle.
Haorong He; Huadong Yin; Xueke Yu; Yao Zhang; Menggen Ma; Diyan Li; Qing Zhu. PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway. Animals 2021, 11, 1016 .
AMA StyleHaorong He, Huadong Yin, Xueke Yu, Yao Zhang, Menggen Ma, Diyan Li, Qing Zhu. PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway. Animals. 2021; 11 (4):1016.
Chicago/Turabian StyleHaorong He; Huadong Yin; Xueke Yu; Yao Zhang; Menggen Ma; Diyan Li; Qing Zhu. 2021. "PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway." Animals 11, no. 4: 1016.
Zearalenone (ZEA) is a nonsteroidal estrogenic mycotoxin found in several food commodities worldwide. ZEA causes reproductive disorders, genotoxicity, and testicular toxicity in animals. However, little is known about the functions of apoptosis and autophagy after exposure to ZEA in granulosa cells. This study investigated the effects of ZEA on chicken granulosa cells. The results show that ZEA at different doses significantly inhibited the growth of chicken granulosa cells by inducing apoptosis. ZEA treatment up-regulated Bax and downregulated Bcl-2 expression, promoted cytochrome c release into the cytosol, and triggered mitochondria-mediated apoptosis. Consequently, caspase-9 and downstream effector caspase-3 were activated, resulting in chicken granulosa cells apoptosis. ZEA treatment also upregulated LC3-II and Beclin-1 expression, suggesting that ZEA induced a high level of autophagy. Pretreatment with chloroquine (an autophagy inhibitor) and rapamycin (an autophagy inducer) increased and decreased the rate of apoptosis, respectively, in contrast with other ZEA-treated groups. Autophagy delayed apoptosis in the ZEA-treated cells. Therefore, autophagy may prevent cells from undergoing apoptosis by reducing ZEA-induced cytotoxicity. In addition, our results further show that the autophagy was stimulated by ZEA through PI3K-AKT-mTOR and MAPK signaling pathways in chicken granulosa cells.
Yifeng Zhu; Heng Wang; Jianping Wang; Shunshun Han; Yao Zhang; Menggen Ma; Qing Zhu; Keying Zhang; Huadong Yin. Zearalenone Induces Apoptosis and Cytoprotective Autophagy in Chicken Granulosa Cells by PI3K-AKT-mTOR and MAPK Signaling Pathways. Toxins 2021, 13, 199 .
AMA StyleYifeng Zhu, Heng Wang, Jianping Wang, Shunshun Han, Yao Zhang, Menggen Ma, Qing Zhu, Keying Zhang, Huadong Yin. Zearalenone Induces Apoptosis and Cytoprotective Autophagy in Chicken Granulosa Cells by PI3K-AKT-mTOR and MAPK Signaling Pathways. Toxins. 2021; 13 (3):199.
Chicago/Turabian StyleYifeng Zhu; Heng Wang; Jianping Wang; Shunshun Han; Yao Zhang; Menggen Ma; Qing Zhu; Keying Zhang; Huadong Yin. 2021. "Zearalenone Induces Apoptosis and Cytoprotective Autophagy in Chicken Granulosa Cells by PI3K-AKT-mTOR and MAPK Signaling Pathways." Toxins 13, no. 3: 199.
The authors wish to make the following corrections to their paper
Jing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84. Animals 2020, 10, 2322 .
AMA StyleJing Zhao, Xiaoxu Shen, Xinao Cao, Haorong He, Shunshun Han, Yuqi Chen, Can Cui, Yuanhang Wei, Yan Wang, Diyan Li, Qing Zhu, Huadong Yin. Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84. Animals. 2020; 10 (12):2322.
Chicago/Turabian StyleJing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. 2020. "Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84." Animals 10, no. 12: 2322.
Fibromodulin (Fmod), which is an extracellular matrix protein, belongs to the extracellular matrix small-leucine-rich proteoglycan family. Fmod is abundantly expressed in muscles and connective tissues and is involved in biological regulation processes, including cell apoptosis, cell adhesion, and modulation of cytokine activity. Fmod is the main regulator of myostatin, which controls the development of muscle cells, but its regulatory path is unknown. Chicken models are ideal for studying embryonic skeletal muscle development; therefore, to investigate the mechanism of Fmod in muscle development, Fmod-silenced and Fmod-overexpressed chicken myoblasts were constructed. The results showed that Fmod plays a positive role in differentiation by detecting the expression of myogenic differentiation markers, immunofluorescence of MyHC protein, and myotube formation in myoblasts. Fmod regulates expression of atrophy-related genes to alleviate muscle atrophy, which was confirmed by histological analysis of breast muscles in Fmod-modulated chicks in vivo. Additionally, genes differentially expressed between Fmod knockdown and normal myoblasts were enriched in the signaling pathway of transforming growth factor β (TGF-β). Both Fmod-silenced and Fmod-overexpressed myoblasts regulated the expression of TGFBR1 and p-Smad3. Thus, Fmod can promote differentiation but not proliferation of myoblasts by regulating the TGF-β signaling pathway, which may serve a function in muscular atrophy.
Huadong Yin; Can Cui; Shunshun Han; Yuqi Chen; Jing Zhao; Haorong He; Diyan Li; Qing Zhu. Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway. Animals 2020, 10, 1477 .
AMA StyleHuadong Yin, Can Cui, Shunshun Han, Yuqi Chen, Jing Zhao, Haorong He, Diyan Li, Qing Zhu. Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway. Animals. 2020; 10 (9):1477.
Chicago/Turabian StyleHuadong Yin; Can Cui; Shunshun Han; Yuqi Chen; Jing Zhao; Haorong He; Diyan Li; Qing Zhu. 2020. "Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway." Animals 10, no. 9: 1477.
MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that post-transcriptionally regulate expression of their target genes. Emerging evidence demonstrates that miRNAs are important regulators in the development of skeletal muscle satellite cells (SMSCs). Our previous research showed that gga-miR-3525 is differentially expressed in breast muscle of broilers (high growth rate) and layers (low growth rate). In this study, we report a new role for gga-miR-3525 as a myogenic miRNA that regulates skeletal muscle development in chickens. Exogenous increases in the expression of gga-miR-3525 significantly inhibited proliferation and differentiation of SMSCs, whereas the opposite effects were observed in gga-miR-3525 knockdown SMSCs. We confirmed that PDLIM3 (PDZ and LIM domain 3) is a target gene of gga-miR-3525 that can promote proliferation and differentiation of SMSCs. We found that PDLIM3 overexpression elevated the abundance of phosphorylated (p-)p38 protein but that the gga-miR-3525 mimic and p38-MAPK inhibitor (SB203580) weakened the activation of p-p38. Furthermore, treatment with SB203580 reduced the promoting effect of PDLIM3 on SMSC proliferation and differentiation. Overall, our results indicate that gga-miR-3525 regulates the proliferation and differentiation of SMSCs by targeting PDLIM3 via the p38/MAPK signaling pathway in chickens.
Huadong Yin; Jing Zhao; Haorong He; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. Gga-miR-3525 Targets PDLIM3 through the MAPK Signaling Pathway to Regulate the Proliferation and Differentiation of Skeletal Muscle Satellite Cells. International Journal of Molecular Sciences 2020, 21, 5573 .
AMA StyleHuadong Yin, Jing Zhao, Haorong He, Yuqi Chen, Yan Wang, Diyan Li, Qing Zhu. Gga-miR-3525 Targets PDLIM3 through the MAPK Signaling Pathway to Regulate the Proliferation and Differentiation of Skeletal Muscle Satellite Cells. International Journal of Molecular Sciences. 2020; 21 (15):5573.
Chicago/Turabian StyleHuadong Yin; Jing Zhao; Haorong He; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. 2020. "Gga-miR-3525 Targets PDLIM3 through the MAPK Signaling Pathway to Regulate the Proliferation and Differentiation of Skeletal Muscle Satellite Cells." International Journal of Molecular Sciences 21, no. 15: 5573.
Immunoglobulin superfamily containing leucine-rich repeat (Islr) contains an Ig-like domain, an LRR motif, and a transmembrane domain and is highly expressed in various chicken tissues. Although Islr has known roles in muscle regeneration, its role in the regulation of muscle atrophy has not been studied. In this study, we constructed Islr-silenced or Islr-overexpressed myoblasts to investigate its role during the differentiation of myoblasts into myotubes. The results showed that Islr was highly expressed in chicken skeletal muscle tissue and regulated myoblast differentiation, but not proliferation. Islr regulated the expression of atrophy-related genes including atrogin-1 and MuRF-1, and could rescue dexamethasone-induced atrophy in myoblasts and myotubes. Western blot analysis indicated that Islr participates in myoblast atrophy through IGF/PI3K/AKT-FOXO signaling. Meanwhile, the expression of caspase-8 and caspase-9 increased in Islr-silenced groups, indicating its role in cell viability. Taken together, these data suggested that Islr plays an important role in myoblasts differentiation, and which can alleviate skeletal muscle atrophy and prevents muscle cell apoptosis via IGF/PI3K/AKT-FOXO signaling pathway.
Can Cui; Shunshun Han; Xiaoxu Shen; Haorong He; Yuqi Chen; Jing Zhao; Yuanhang Wei; Yan Wang; Qing Zhu; Diyan Li; Huadong Yin. ISLR regulates skeletal muscle atrophy via IGF1-PI3K/Akt-Foxo signaling pathway. Cell and Tissue Research 2020, 381, 479 -492.
AMA StyleCan Cui, Shunshun Han, Xiaoxu Shen, Haorong He, Yuqi Chen, Jing Zhao, Yuanhang Wei, Yan Wang, Qing Zhu, Diyan Li, Huadong Yin. ISLR regulates skeletal muscle atrophy via IGF1-PI3K/Akt-Foxo signaling pathway. Cell and Tissue Research. 2020; 381 (3):479-492.
Chicago/Turabian StyleCan Cui; Shunshun Han; Xiaoxu Shen; Haorong He; Yuqi Chen; Jing Zhao; Yuanhang Wei; Yan Wang; Qing Zhu; Diyan Li; Huadong Yin. 2020. "ISLR regulates skeletal muscle atrophy via IGF1-PI3K/Akt-Foxo signaling pathway." Cell and Tissue Research 381, no. 3: 479-492.
As bioinformatic approaches have been developed, it has been demonstrated that microRNAs (miRNAs) are involved in the formation of muscles and play important roles in regulation of muscle cell proliferation and differentiation. Previously, it has been demonstrated that miR-148a-3p is one of the most abundant miRNAs in chicken skeletal muscle. Here, we build on that work and demonstrate that miR-148a-3p is important in the control of differentiation of chicken skeletal muscle satellite cells (SMSCs). Elevated expression of miR-148a-3p significantly promoted differentiation and inhibited apoptosis of SMSCs but did not affect proliferation. Furthermore, it was observed that the mesenchyme homeobox 2 (Meox2) is a target gene of miR-148a-3p and that miR-148a-3p can down-regulate expression of Meox2, which promote differentiation of SMSCs and suppress apoptosis. Furthermore, miR-148a-3p overexpression encouraged activation of the PI3K/AKT signaling pathway, which could be recovered by overexpression of Meox2. Overall, these findings suggest that microRNA-148a-3p is a potent promoter of myogenesis via direct targeting of Meox2 and increase of the PI3K/AKT signaling pathway in chicken SMSCs.
Huadong Yin; Haorong He; Xinao Cao; Xiaoxu Shen; Shunshun Han; Can Cui; Jing Zhao; Yuanhang Wei; Yuqi Chen; Lu Xia; Yan Wang; Diyan Li; Qing Zhu. MiR-148a-3p Regulates Skeletal Muscle Satellite Cell Differentiation and Apoptosis via the PI3K/AKT Signaling Pathway by Targeting Meox2. Frontiers in Genetics 2020, 11, 1 .
AMA StyleHuadong Yin, Haorong He, Xinao Cao, Xiaoxu Shen, Shunshun Han, Can Cui, Jing Zhao, Yuanhang Wei, Yuqi Chen, Lu Xia, Yan Wang, Diyan Li, Qing Zhu. MiR-148a-3p Regulates Skeletal Muscle Satellite Cell Differentiation and Apoptosis via the PI3K/AKT Signaling Pathway by Targeting Meox2. Frontiers in Genetics. 2020; 11 ():1.
Chicago/Turabian StyleHuadong Yin; Haorong He; Xinao Cao; Xiaoxu Shen; Shunshun Han; Can Cui; Jing Zhao; Yuanhang Wei; Yuqi Chen; Lu Xia; Yan Wang; Diyan Li; Qing Zhu. 2020. "MiR-148a-3p Regulates Skeletal Muscle Satellite Cell Differentiation and Apoptosis via the PI3K/AKT Signaling Pathway by Targeting Meox2." Frontiers in Genetics 11, no. : 1.
MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (TGF-β2) was identified as a target gene of miR-200a-3p, and which turned out to inhibit differentiation and proliferation, and promote apoptosis of SMSCs. Exogenous TGF-β2 increased the abundances of phosphorylated SMAD2 and SMAD3 proteins, and a miR-200a-3p mimic weakened this effect. The TGF-β2 inhibitor treatment reduced the promotional and inhibitory effects of miR-200a-3p on SMSC differentiation and apoptosis, respectively. Our results indicate that miRNAs are abundantly expressed during embryonic skeletal muscle development, and that miR-200a-3p promotes SMSC development by targeting TGF-β2 and regulating the TGF-β2/SMAD signaling pathway.
Huadong Yin; Haorong He; Xiaoxu Shen; Shuyue Tang; Jing Zhao; Xinao Cao; Shunshun Han; Can Cui; Yuqi Chen; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu. MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway. International Journal of Molecular Sciences 2020, 21, 3274 .
AMA StyleHuadong Yin, Haorong He, Xiaoxu Shen, Shuyue Tang, Jing Zhao, Xinao Cao, Shunshun Han, Can Cui, Yuqi Chen, Yuanhang Wei, Yan Wang, Diyan Li, Qing Zhu. MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway. International Journal of Molecular Sciences. 2020; 21 (9):3274.
Chicago/Turabian StyleHuadong Yin; Haorong He; Xiaoxu Shen; Shuyue Tang; Jing Zhao; Xinao Cao; Shunshun Han; Can Cui; Yuqi Chen; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu. 2020. "MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway." International Journal of Molecular Sciences 21, no. 9: 3274.
Aflatoxin B1 (AFB1) leads to a major risk to poultry and its residues in meat products can also pose serious threat to human health. In this study, after feeding 165-day-old Roman laying hens for 35 days, the toxic effects of aflatoxin B1 at different concentrations were evaluated. The purpose of this study was to explore the mechanism of liver toxicosis responses to AFB1. We found that highly toxic group exposure resulted in liver fat deposition, increased interstitial space, and hepatocyte apoptosis in laying hens. Furthermore, a total of 164 differentially expressed lnRNAs and 186 differentially expressed genes were found to be highly correlated (Pearson Correlation Coefficient > 0.80, p-value < 0.05) by sequencing the transcriptome of control (CB) and highly toxic group (TB3) chickens. We also identify 29 differentially expressed genes and 19 miRNAs that have targeted regulatory relationships. Based on the liver cell apoptosis and fatty liver syndrome that this research focused on, we found that the highly toxic AFB1 led to dysregulation of the expression of PPARG and BCL6. They are cis-regulated by TU10057 and TU45776, respectively. PPARG was the target gene of gga-miR-301a-3p, gga-miR-301b-3p, and BCL6 was the target gene of gga-miR-190a-3p. In summary, highly toxic AFB1 affects the expression levels of protein-coding genes and miRNAs in the liver of Roman layer hens, as well as the expression level of long non-coding RNA in the liver, which upregulates the expression of PPARG and downregulates the expression of Bcl-6. Our study provides information on possible genetic regulatory networks in AFB1-induced hepatic fat deposition and hepatocyte apoptosis.
Xueqin Liu; Shailendra Kumar Mishra; Tao Wang; Zhongxian Xu; Xiaoling Zhao; Yan Wang; Huadong Yin; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Qing Zhu; Feng Chen; Diyan Li. AFB1 Induced Transcriptional Regulation Related to Apoptosis and Lipid Metabolism in Liver of Chicken. Toxins 2020, 12, 290 .
AMA StyleXueqin Liu, Shailendra Kumar Mishra, Tao Wang, Zhongxian Xu, Xiaoling Zhao, Yan Wang, Huadong Yin, Xiaolan Fan, Bo Zeng, Mingyao Yang, Deying Yang, Qingyong Ni, Yan Li, Mingwang Zhang, Qing Zhu, Feng Chen, Diyan Li. AFB1 Induced Transcriptional Regulation Related to Apoptosis and Lipid Metabolism in Liver of Chicken. Toxins. 2020; 12 (5):290.
Chicago/Turabian StyleXueqin Liu; Shailendra Kumar Mishra; Tao Wang; Zhongxian Xu; Xiaoling Zhao; Yan Wang; Huadong Yin; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Qing Zhu; Feng Chen; Diyan Li. 2020. "AFB1 Induced Transcriptional Regulation Related to Apoptosis and Lipid Metabolism in Liver of Chicken." Toxins 12, no. 5: 290.
Noncoding RNAs, especially microRNAs (miRNAs), have been reported to play important roles during skeletal muscle development and regeneration. Our previous sequencing data revealed that miR-99a-5p is one of the most abundant miRNAs in chicken breast muscle. The purpose of this study was to reveal the regulatory mechanism of miR-99a-5p in the proliferation and differentiation of chicken skeletal muscle satellite cells (SMSCs). Through the investigation of cell proliferation activity, cell cycle progression, and 5-ethynyl-29-deoxyuridine (EdU) assay, we found that miR-99a-5p can significantly promote the proliferation of SMSCs. Moreover, we found that miR-99a-5p can inhibit myotube formation by decreasing the expression of muscle cell differentiation marker genes. After miR-99a-5p target gene scanning, we confirmed that miR-99a-5p directly targets the 3′ untranslated region (UTR) of myotubularin-related protein 3 (MTMR3) and regulates its expression level during chicken SMSC proliferation and differentiation. We also explored the role of MTMR3 in muscle development and found that its knockdown significantly facilitates the proliferation but represses the differentiation of SMSCs, which is opposite to the effects of miR-99a-5p. Overall, we demonstrated that miR-99a-5p regulates the proliferation and differentiation of SMSCs by targeting MTMR3.
Xinao Cao; Shuyue Tang; Fei Du; Hao Li; Xiaoxu Shen; Diyan Li; Yan Wang; Zhichao Zhang; Lu Xia; Qing Zhu; Huadong Yin. miR-99a-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting MTMR3 in Chicken. Genes 2020, 11, 369 .
AMA StyleXinao Cao, Shuyue Tang, Fei Du, Hao Li, Xiaoxu Shen, Diyan Li, Yan Wang, Zhichao Zhang, Lu Xia, Qing Zhu, Huadong Yin. miR-99a-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting MTMR3 in Chicken. Genes. 2020; 11 (4):369.
Chicago/Turabian StyleXinao Cao; Shuyue Tang; Fei Du; Hao Li; Xiaoxu Shen; Diyan Li; Yan Wang; Zhichao Zhang; Lu Xia; Qing Zhu; Huadong Yin. 2020. "miR-99a-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting MTMR3 in Chicken." Genes 11, no. 4: 369.
MicroRNAs are evolutionarily conserved, small non-coding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. We previously found that miR-9-5p is abundantly expressed in chicken skeletal muscle. Here, we demonstrate a new role for miR-9-5p as a myogenic microRNA that regulates skeletal muscle development. The overexpression of miR-9-5p significantly inhibited the proliferation and differentiation of skeletal muscle satellite cells (SMSCs), whereas miR-9-5p inhibition had the opposite effect. We show that insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) is a target gene of miR-9-5p, using dual-luciferase assays, RT-qPCR, and Western Blotting, and that it promotes proliferation and differentiation of SMSCs. In addition, we found that IGF2BP3 regulates IGF-2 expression, using overexpression and knockdown studies. We show that Akt is activated by IGF2BP3 and is essential for IGF2BP3-induced cell development. Together, our results indicate that miR-9-5p could regulate the proliferation and differentiation of myoblasts by targeting IGF2BP3 through IGF-2 and that this activity results in the activation of the PI3K/Akt signaling pathway in skeletal muscle cells.
Huadong Yin; Haorong He; Xiaoxu Shen; Jing Zhao; Xinao Cao; Shunshun Han; Can Cui; Yuqi Chen; Yuanhang Wei; Lu Xia; Yan Wang; Diyan Li; Qing Zhu. miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway. International Journal of Molecular Sciences 2020, 21, 1655 .
AMA StyleHuadong Yin, Haorong He, Xiaoxu Shen, Jing Zhao, Xinao Cao, Shunshun Han, Can Cui, Yuqi Chen, Yuanhang Wei, Lu Xia, Yan Wang, Diyan Li, Qing Zhu. miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway. International Journal of Molecular Sciences. 2020; 21 (5):1655.
Chicago/Turabian StyleHuadong Yin; Haorong He; Xiaoxu Shen; Jing Zhao; Xinao Cao; Shunshun Han; Can Cui; Yuqi Chen; Yuanhang Wei; Lu Xia; Yan Wang; Diyan Li; Qing Zhu. 2020. "miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway." International Journal of Molecular Sciences 21, no. 5: 1655.
To study the influence of the PGC-1β gene on chicken adipocyte proliferation and differentiation, we constructed RNA interference (RNAi) vectors that target the PGC-1β gene and transfected these vectors into adipocytes. Oil Red O staining and a CCK-8 cell kit were used to determine cell triglyceride accumulation status and cell proliferation after transfection, respectively. The mRNA abundances of PGC-1β and adipocyte-differentiation-related genes (PPARγ, C/EBPα, SREBP-1c, FAS, and A-FABP) were detected by real-time PCR. The results showed that the mRNA and protein abundances of PGC-1β in PGC-1β-shRNA transfected adipocytes were significantly lower than those in the control. Interference decreased cell differentiation, but did not depress the cell proliferation. PGC-1β interference impeded the triglyceride accumulation, the mRNA expression levels of nuclear receptors PPARγ and SREBP-1c, and fatty acid synthetase (FAS), and both proteins PPARγ and SREBP-1c, and the fatty acids transporting protein A-FABP. Generally, PGC-1β modulated the cell differentiation and triglyceride accumulation in chicken adipocytes.
Weizhen Song; Chenglin Zhong; Yuncong Yuan; Qing Zhu; Yan Wang; Huadong Yin; Diyan Li; Zhichao Zhang; Gang Shu; Chaowu Yang; Huarui Du; Xiaosong Jiang; Xiaoling Zhao. Peroxisome proliferator-activated receptor-coactivator 1-beta (PGC-1β) modulates the expression of genes involved in adipogenesis during preadipocyte differentiation in chicken. Gene 2020, 741, 144516 .
AMA StyleWeizhen Song, Chenglin Zhong, Yuncong Yuan, Qing Zhu, Yan Wang, Huadong Yin, Diyan Li, Zhichao Zhang, Gang Shu, Chaowu Yang, Huarui Du, Xiaosong Jiang, Xiaoling Zhao. Peroxisome proliferator-activated receptor-coactivator 1-beta (PGC-1β) modulates the expression of genes involved in adipogenesis during preadipocyte differentiation in chicken. Gene. 2020; 741 ():144516.
Chicago/Turabian StyleWeizhen Song; Chenglin Zhong; Yuncong Yuan; Qing Zhu; Yan Wang; Huadong Yin; Diyan Li; Zhichao Zhang; Gang Shu; Chaowu Yang; Huarui Du; Xiaosong Jiang; Xiaoling Zhao. 2020. "Peroxisome proliferator-activated receptor-coactivator 1-beta (PGC-1β) modulates the expression of genes involved in adipogenesis during preadipocyte differentiation in chicken." Gene 741, no. : 144516.
T-2 toxin is type A trichothecenes mycotoxin, which produced by fusarium species in cereal grains. T-2 toxin has been shown to induce a series of toxic effects on the health of human and animal, such as immunosuppression and carcinogenesis. Previous study has proven that T-2 toxin caused hepatotoxicity in chicken, but the regulatory mechanism is unclear. In the present study, we assessed the toxicological effect of T-2 toxin on apoptosis and autophagy in hepatocytes. The total of 120 1-day-old healthy broilers were allocated randomly into four groups and reared for 21 day with complete feed containing 0 mg/kg, 0.5 mg/kg, 1 mg/kg or 2 mg/kg T-2 toxin, respectively. The results showed that the apoptosis rate and pathological changes degree hepatocytes were aggravated with the increase of T-2 toxin. At the molecular mechanism level, T-2 toxin induced mitochondria-mediated apoptosis by producing reactive oxygen species, promoting cytochrome c translocation between the mitochondria and cytoplasm, and thus promoting apoptosomes formation. Meanwhile, the expression of the autophagy-related protein, ATG5, ATG7 and Beclin-1, and the LC3-II/LC3-I ratio were increased, while p62 was downregulated, suggesting T-2 toxin caused autophagy in hepatocytes. Further experiments demonstrated that the PI3K/AKT/mTOR signal may be participated in autophagy induced by T-2 toxin in chicken hepatocytes. These data suggest a possible underlying molecular mechanism for T-2 toxin that induces apoptosis and autophagy in chicken hepatocytes
Huadong Yin; Shunshun Han; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins 2020, 12, 90 .
AMA StyleHuadong Yin, Shunshun Han, Yuqi Chen, Yan Wang, Diyan Li, Qing Zhu. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins. 2020; 12 (2):90.
Chicago/Turabian StyleHuadong Yin; Shunshun Han; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. 2020. "T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes." Toxins 12, no. 2: 90.
CSRP3/MLP (cysteine-rich protein 3/muscle Lim protein), a member of the cysteine-rich protein family, is a muscle-specific LIM-only factor specifically expressed in skeletal muscle. CSRP3 is critical in maintaining the structure and function of normal muscle. To investigate the mechanism of disease in CSRP3 myopathy, we performed siRNA-mediated CSRP3 knockdown in chicken primary myoblasts. CSRP3 silencing resulted in the down-regulation of the expression of myogenic genes and the up-regulation of atrophy-related gene expressions. We found that CSRP3 interacted with LC3 protein to promote the formation of autophagosomes during autophagy. CSRP3-silencing impaired myoblast autophagy, as evidenced by inhibited autophagy-related ATG5 and ATG7 mRNA expression levels, and inhibited LC3II and Beclin-1 protein accumulation. In addition, impaired autophagy in CSRP3-silenced cells resulted in increased sensitivity to apoptosis cell death. CSRP3-silenced cells also showed increased caspase-3 and caspase-9 cleavage. Moreover, apoptosis induced by CSRP3 silencing was alleviated after autophagy activation. Together, these results indicate that CSRP3 promotes the correct formation of autophagosomes through its interaction with LC3 protein, which has an important role in skeletal muscle remodeling and maintenance.
Can Cui; Shunshun Han; Shuyue Tang; Haorong He; Xiaoxu Shen; Jing Zhao; Yuqi Chen; Yuanhang Wei; Yan Wang; Qing Zhu; Diyan Li; And Huadong Yin. The Autophagy Regulatory Molecule CSRP3 Interacts with LC3 and Protects Against Muscular Dystrophy. International Journal of Molecular Sciences 2020, 21, 749 .
AMA StyleCan Cui, Shunshun Han, Shuyue Tang, Haorong He, Xiaoxu Shen, Jing Zhao, Yuqi Chen, Yuanhang Wei, Yan Wang, Qing Zhu, Diyan Li, And Huadong Yin. The Autophagy Regulatory Molecule CSRP3 Interacts with LC3 and Protects Against Muscular Dystrophy. International Journal of Molecular Sciences. 2020; 21 (3):749.
Chicago/Turabian StyleCan Cui; Shunshun Han; Shuyue Tang; Haorong He; Xiaoxu Shen; Jing Zhao; Yuqi Chen; Yuanhang Wei; Yan Wang; Qing Zhu; Diyan Li; And Huadong Yin. 2020. "The Autophagy Regulatory Molecule CSRP3 Interacts with LC3 and Protects Against Muscular Dystrophy." International Journal of Molecular Sciences 21, no. 3: 749.
The development of skeletal muscle satellite cells (SMSCs) is a complex process that could be regulated by many genes. Previous studies have shown that Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation, differentiation, and apoptosis in mouse. However, the function of HDAC4 in chicken muscle development is still unknown. Given that chicken is a very important meat-producing animal that is also an ideal model to study skeletal muscle development, we explored the functions of HDAC4 in chicken SMSCs after the interference of HDAC4. The results showed that HDAC4 was enriched in embryonic skeletal muscle, and it was highly expressed in embryonic muscle than in postnatal muscles. Meanwhile, knockdown of HDAC4 could significantly inhibit the proliferation and differentiation of chicken SMSCs but had no effect on the apoptosis of SMSCs as observed in a series of experiment conducted in vitro. These results indicated that HDAC4 might play a positive role in chicken skeletal muscle growth and development.
Jing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84 .
AMA StyleJing Zhao, Xiaoxu Shen, Xinao Cao, Haorong He, Shunshun Han, Yuqi Chen, Can Cui, Yuanhang Wei, Yan Wang, Diyan Li, Qing Zhu, Huadong Yin. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals. 2020; 10 (1):84.
Chicago/Turabian StyleJing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. 2020. "HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells." Animals 10, no. 1: 84.
Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. HSPGs interact with growth factors and receptors through heparan sulfate (HS) chains. The sulfation pattern of heparan sulfate chains influences signaling events mediated by heparan sulfate proteoglycans located on the cell surface. SULF1 and SULF2 are two endo-sulfatases that can cleave specific 6-O-sulfate groups within the heparan chains. To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. The SULF1 and SULF2 transcripts were widely distributed in various tissues. Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. Therefore, our findings increase our knowledge of sulfatase expression diversity and provide a solid basis for further research concerning this molecular mechanism.
L He; H Xu; F Ye; H Yu; Y Lu; H Yin; X Zhao; Q Zhu; Y Wang. Expression Pattern of Sulf1 and Sulf2 in Chicken Tissues and Characterization of Their Expression During Different Periods in Skeletal Muscle Satellite Cells. Brazilian Journal of Poultry Science 2020, 22, 1 .
AMA StyleL He, H Xu, F Ye, H Yu, Y Lu, H Yin, X Zhao, Q Zhu, Y Wang. Expression Pattern of Sulf1 and Sulf2 in Chicken Tissues and Characterization of Their Expression During Different Periods in Skeletal Muscle Satellite Cells. Brazilian Journal of Poultry Science. 2020; 22 (3):1.
Chicago/Turabian StyleL He; H Xu; F Ye; H Yu; Y Lu; H Yin; X Zhao; Q Zhu; Y Wang. 2020. "Expression Pattern of Sulf1 and Sulf2 in Chicken Tissues and Characterization of Their Expression During Different Periods in Skeletal Muscle Satellite Cells." Brazilian Journal of Poultry Science 22, no. 3: 1.
Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and treatment of AL, thus screening of significant immune markers is needed to promote the development of disease-resistant breeds. In this study, data-independent acquisition (DIA) technology was used to detect the DEPs of three breeds of chicken according to different comparison to investigate the potential markers. Results showed special DEPs for spleen development of each breed were detected, such as PCNT, DDB2, and ZNF62. These DEPs were involved in intestinal immune network used in production of IgA signaling pathways and related to immune response which can be used as potential markers for spleen development in different breeds. The DEPs such as RAB44 and TPN involved in viral myocarditis, transcriptional misregulation in cancer, and tuberculosis can be used as potential markers of spleen immune response after ALV-J infection in chickens. Pair-wise analysis was performed for the three breeds after the infection of ALV-J. The proteins such as RFX1, TAF10, and VH1 were differently expressed between three breeds. These DEPs involved in antigen processing and expression, acute myelogenous leukemia, and viral carcinogenesis can be used as potential immune markers after ALV-J infection of different genetic backgrounds. The screening of potential markers at protein level provides a strong theoretical research basis for disease resistance breeding in poultry.
Fei Ye; Yan Wang; Qijian He; Zhaoshuo Wang; Enyue Ma; Shiliang Zhu; Heling Yu; Huadong Yin; Xiaoling Zhao; Diyan Li; Hengyong Xu; Hua Li; Qing Zhu. Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic. Virulence 2020, 11, 1158 -1176.
AMA StyleFei Ye, Yan Wang, Qijian He, Zhaoshuo Wang, Enyue Ma, Shiliang Zhu, Heling Yu, Huadong Yin, Xiaoling Zhao, Diyan Li, Hengyong Xu, Hua Li, Qing Zhu. Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic. Virulence. 2020; 11 (1):1158-1176.
Chicago/Turabian StyleFei Ye; Yan Wang; Qijian He; Zhaoshuo Wang; Enyue Ma; Shiliang Zhu; Heling Yu; Huadong Yin; Xiaoling Zhao; Diyan Li; Hengyong Xu; Hua Li; Qing Zhu. 2020. "Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic." Virulence 11, no. 1: 1158-1176.