Hericium erinaceus is a medicinal fungal species that produces the active biological metabolite erinacine A with strong antioxidant activity. The classical extraction techniques used to date to obtain metabolites from this fungal species require high consumption of resources and energy and, in the end, prove to be expensive and inefficient, especially on a biomedical scale. The aim of this research is based on the development of an ultrasonic extraction (UE) method for the identification and extraction of biological compounds with high antioxidant activity from the mycelia of H. erinaceus biomass developed through a solid cultivation process. The extraction process was optimized by varying parameters to determine the best extraction yield of metabolites involved in such antioxidant activity, using the response surface methodology (RSM). The physicochemical analyses were oriented towards the investigation of polyphenols, flavonoids, and the diterpenoid erinacine A. It is highlighted that there is a very good mutual connection between the concentration of polyphenols and flavonoids in the extracts studied and the diterpenoid erinacine A. Also, this study describes an efficient and qualitative extraction method for extracting natural antioxidants from the H. erinaceus mushroom, since toxic solvents were not used in the developed extraction procedure. This biomass can be used both as a food source and as a possible phytotherapeutic tool in the prevention or treatment of various neurodegenerative disorders that require drugs with strong antioxidant activity.

We report a study on the biocompatibility vs. thickness in the case of titanium nitride (TiN) films synthesized on 410 medical grade stainless steel substrates by pulsed laser deposition. The films were grown in a nitrogen atmosphere, and their in vitro cytotoxicity was assessed according to ISO 10993-5 [1]. Extensive physical-chemical analyses have been carried out on the deposited structures with various thicknesses in order to explain the differences in biological behavior: profilometry, scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy (XPS), X-ray diffraction and surface energy measurements. XPS revealed the presence of titanium oxynitride beside TiN in amounts that vary with the film thickness. The cytocompatibility of films seems to be influenced by their TiN surface content. The thinner films seem to be more suitable for medical applications, due to the combined high values of bonding strength and superior cytocompatibility.

The plasma electrolytic oxidation (PEO) surface modification technique was employed for improving the mechanical and anti-corrosion properties of the AZ63 magnesium alloy. Different PEO processing times (5, 10 and 20 min) in a 10 g/L NaAlO2 electrolyte, with no other additives, led to the formation of ceramic coatings with mean thicknesses between 15 and 37 microns. Scanning electron microscopy (SEM) showed that the porosity of the coatings decreased with processing time, but an increase in roughness was observed. X-Ray diffraction phase analysis indicated a coating structure composed of majority magnesium aluminate spinel. The corrosion rate of the coated samples decreased with an order of magnitude compared with the bare alloy. The average micro-hardness values of the PEO-coated samples was up to five times higher than those of the AZ63 alloy.

The Joint European Torus (JET) fusion reactor was upgraded to the metallic wall configuration in 2011 which consisted of bulk beryllium (Be) tiles in the main chamber and bulk tungsten (W) and W-coated CFC tiles in the divertor (Matthews G.F. et al 2011 Phys. Scr. T148 014001). During each campaign, a series of wall damages were observed; on the upper dump plates (UDP) positioned to the top part of the vessel walls and on the inner wall—mainly affecting the inner wall guard limiters (IWGL). In both cases, it was concluded that the causes of these damages were unmitigated plasma disruptions. In the case of JET with the metallic wall configuration, most of these plasma disruptions were intentionally provoked. The overall objective was to study the behaviour of these phenomena, in order to assess their impact on the wall, improve understanding of morphological material changes, and—based on that—to develop, implement and test mitigation techniques for their prospective use on ITER. The current results bring additional information on the effects of the unmitigated plasma disruptions on the UDPs and are a significant extension of work presented in (Jepu et al 2019 Nucl. Fusion 59 086009) where the scale of the damage after three operational campaigns on the Be top limiters of JET was highlighted. In addition, new data is presented on the damaging effect that the high energetic runaway electrons had on the Be IWGL in JET.