This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 10⁵ PFU/mL to 1.5×10⁷ PFU/mL, more than 1.0 × 10⁵ PFU/mL to 1.0 × 10⁷ PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 10³ PFU/mL, three orders of magnitude lower than 2.85 × 10⁶ PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.

This work presents a systematic approach to formulating UV curable ionomer coatings that can be used as ion-exchange membranes when they are applied on porous substrates. Ion-exchange membranes fabricated in this way can be a cost-effective alternative to perfluorosulfonic acid membranes, such as Nafion and similar thin ionomer film membranes. Hierarchically structured coated membranes find applications for energy storage and conversion (organic redox flow batteries and artificial photosynthesis cells) and separation processes (electrodialysis). Designing the ionomer precursor for membrane formulation requires the introduction of compounds with drastically different properties into a liquid mixture. Hansen solubility theory was used to find the solvents to compatibilize main formulation components: acrylic sulfone salt (3-sulfopropyl methacrylate potassium salt) and hexafunctional polyester acrylate cross-linker (Ebecryl 830), otherwise nonmiscible or mutely soluble. Among the identified suitable solvents, acrylic acid and acetic acid allowed for optimal mixing of the components and reaching the highest levels of sulfonic group content, providing the desired ion-exchange capacity. Interestingly, they represented a case of a reactive and nonreactive solvent since acrylic acid was built into the ionomer during the UV curing step. Properties of the two membrane variants were compared. Samples fabricated with acetic acid exhibit improved handleability compared with the case of acrylic acid. Acetic acid yielded a lower area-specific resistance (6.4 ± 0.17 Ohm·cm²) compared to acrylic acid (12.1 ± 0.16 Ohm·cm² in 0.5 M NaCl). This was achieved without severely suppressing the selectivity of the membrane, which was standing at 93.4 and 96.4% for preparation with acetic and acrylic acid, respectively.