Bacteria that surround plant roots and exert beneficial effects on plant growth are known as plant growth-promoting rhizobacteria (PGPR). In addition to the plant growth-promotion, PGPR also imparts resistance against salinity and oxidative stress and needs to be studied. Such PGPR can function as dynamic bioinoculants under salinity conditions. The present study reports the isolation of phytase positive multifarious Klebsiella variicola SURYA6 isolated from wheat rhizosphere in Kolhapur, India. The isolate produced various plant growth-promoting (PGP), salinity ameliorating, and antioxidant traits. It produced organic acid, yielded a higher phosphorous solubilization index (9.3), maximum phytase activity (376.67 ± 2.77 U/mL), and copious amounts of siderophore (79.0%). The isolate also produced salt ameliorating traits such as indole acetic acid (78.45 ± 1.9 µg/mL), 1 aminocyclopropane-1-carboxylate deaminase (0.991 M/mg/h), and exopolysaccharides (32.2 ± 1.2 g/L). In addition to these, the isolate also produced higher activities of antioxidant enzymes like superoxide dismutase (13.86 IU/mg protein), catalase (0.053 IU/mg protein), and glutathione oxidase (22.12 µg/mg protein) at various salt levels. The isolate exhibited optimum growth and maximum secretion of these metabolites during the log-phase growth. It exhibited sensitivity to a wide range of antibiotics and did not produce hemolysis on blood agar, indicative of its non-pathogenic nature. The potential of K. variicola to produce copious amounts of various PGP, salt ameliorating, and antioxidant metabolites make it a potential bioinoculant for salinity stress management.

As a source of xylan, lignocellulose waste such as wheat bran and aquatic weed Pistia stratiotes were employed, together with potassium nitrate as a nitrogen source, which were optimized using the Box- Behnken design to increase the production of xylooligosaccharides. The yield of xylooligosaccharides increased to 2.05- fold in the optimized medium than non-optimized medium. The produced xylooligosaccharides showed a peak at 890-900 cm-1 confirming the presence of β-glycosidic linkages when detected by Fourier Transform Infra-Red (FT IR). The prebiotic effect of produced xylooligosaccharides was studied on two strains named Lactococcus lactis Cl MN515342 and Lactococcus lactis Cp MN515343 isolated from curd samples. The growth of these Lactococcus strains was found to be stimulated. A log Colony Forming Unit/ml value of 7.813 and 8.146 for Cp and Cl was observed in a medium incorporated with XOS. The Short-Chain Fatty Acid (SCFA) production by Lactococcus lactis in a medium incorporated with xylooligosaccharides was detected by GCMS. The use of lignocellulosic waste for the production of xylooligosaccharides reduces the production cost. The xylooligosaccharides produced by Massilia timonae has shown promising result in stimulating the growth of Lactococcus lactis. Thus, XOS can be incorporated in curd or Yoghurt along with the Lactococcus lactis and used as synbiotic food.

Efforts put in overriding the inulin abundant invader nastiest category I weeds are infeasible that lead into its impermanent confiscation. Hence, their heedful exploitation is obligatory. These invasive weeds have ample amount of inulin, which serves as a renewable, cheap raw substrate for inulinase production. Therefore, they have enticed intention of many researchers toward exploring more idiosyncratic inulinase producing microbial strains that utilize invasive inulin-rich weeds as substrate for fructose liberation. Plenteous industrial applications of inulinases have marked it distinctly crucial in recent biotechnological epoch. This review thus elaborates the literature on infused footprints embedded by the substituted low calorie healthy sweetener in new advancing fields.