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Branka Uzelac completed her Ph.D. in Experimental and Applied Botany at the University of Belgrade, Serbia (2012). She is a member of the Laboratory of Plant Cytology and Histology at the Institute for Biological Research „Siniša Stanković“–National Institute of the Republic of Serbia, University of Belgrade. Her research has generally been in the area of cytology and anatomy of plant development, focusing on cytological aspects of the mechanisms responsible for cell and tissue differentiation. She has published 25 papers in reputed journals.
In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.
Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Slađana Jevremović; Angelina Subotić. Bulb Dormancy In Vitro—Fritillaria meleagris: Initiation, Release and Physiological Parameters. Plants 2021, 10, 902 .
AMA StyleMarija Marković, Milana Trifunović Momčilov, Branka Uzelac, Slađana Jevremović, Angelina Subotić. Bulb Dormancy In Vitro—Fritillaria meleagris: Initiation, Release and Physiological Parameters. Plants. 2021; 10 (5):902.
Chicago/Turabian StyleMarija Marković; Milana Trifunović Momčilov; Branka Uzelac; Slađana Jevremović; Angelina Subotić. 2021. "Bulb Dormancy In Vitro—Fritillaria meleagris: Initiation, Release and Physiological Parameters." Plants 10, no. 5: 902.
Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.
Branka Uzelac; Dragana Stojičić; Snežana Budimir. Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. Reference Series in Phytochemistry 2020, 25 -61.
AMA StyleBranka Uzelac, Dragana Stojičić, Snežana Budimir. Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. Reference Series in Phytochemistry. 2020; ():25-61.
Chicago/Turabian StyleBranka Uzelac; Dragana Stojičić; Snežana Budimir. 2020. "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites." Reference Series in Phytochemistry , no. : 25-61.
The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.
Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Olga Radulović; Snežana Milošević; Slađana Jevremović; Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. Plants 2020, 9, 1573 .
AMA StyleMarija Marković, Milana Trifunović Momčilov, Branka Uzelac, Olga Radulović, Snežana Milošević, Slađana Jevremović, Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. Plants. 2020; 9 (11):1573.
Chicago/Turabian StyleMarija Marković; Milana Trifunović Momčilov; Branka Uzelac; Olga Radulović; Snežana Milošević; Slađana Jevremović; Angelina Subotić. 2020. "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs." Plants 9, no. 11: 1573.
High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
Maja Belić; Snežana Zdravković-Korać; Branka Uzelac; Dušica Ćalić; Suzana Pavlović; Jelena Milojević. Variability in somatic embryo-forming capacity of spinach. Scientific Reports 2020, 10, 1 -10.
AMA StyleMaja Belić, Snežana Zdravković-Korać, Branka Uzelac, Dušica Ćalić, Suzana Pavlović, Jelena Milojević. Variability in somatic embryo-forming capacity of spinach. Scientific Reports. 2020; 10 (1):1-10.
Chicago/Turabian StyleMaja Belić; Snežana Zdravković-Korać; Branka Uzelac; Dušica Ćalić; Suzana Pavlović; Jelena Milojević. 2020. "Variability in somatic embryo-forming capacity of spinach." Scientific Reports 10, no. 1: 1-10.
Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors—ancymidol (A) and paclobutrazol (P)—the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb’s dormancy and its sprouting.
Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Aleksandar Cingel; Snežana Milošević; Slađana Jevremović; Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content. Plants 2020, 9, 1449 .
AMA StyleMarija Marković, Milana Trifunović Momčilov, Branka Uzelac, Aleksandar Cingel, Snežana Milošević, Slađana Jevremović, Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content. Plants. 2020; 9 (11):1449.
Chicago/Turabian StyleMarija Marković; Milana Trifunović Momčilov; Branka Uzelac; Aleksandar Cingel; Snežana Milošević; Slađana Jevremović; Angelina Subotić. 2020. "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content." Plants 9, no. 11: 1449.
The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.
Olga Radulović; Slaviša Stanković; Branka Uzelac; Vojin Tadić; Milana Trifunović-Momčilov; Jelena Lozo; Marija Marković. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. Plants 2020, 9, 599 .
AMA StyleOlga Radulović, Slaviša Stanković, Branka Uzelac, Vojin Tadić, Milana Trifunović-Momčilov, Jelena Lozo, Marija Marković. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. Plants. 2020; 9 (5):599.
Chicago/Turabian StyleOlga Radulović; Slaviša Stanković; Branka Uzelac; Vojin Tadić; Milana Trifunović-Momčilov; Jelena Lozo; Marija Marković. 2020. "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations." Plants 9, no. 5: 599.
Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.
Branka Uzelac; Dragana Stojičić; Snežana Budimir. Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. Reference Series in Phytochemistry 2019, 1 -37.
AMA StyleBranka Uzelac, Dragana Stojičić, Snežana Budimir. Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. Reference Series in Phytochemistry. 2019; ():1-37.
Chicago/Turabian StyleBranka Uzelac; Dragana Stojičić; Snežana Budimir. 2019. "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites." Reference Series in Phytochemistry , no. : 1-37.
In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
Svetlana Tošić; Dragana Stojičić; Violeta Slavkovska; Tatjana Mihailov-Krstev; Bojan Zlatković; Snežana Budimir; Branka Uzelac. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). Planta 2019, 249, 1365 -1377.
AMA StyleSvetlana Tošić, Dragana Stojičić, Violeta Slavkovska, Tatjana Mihailov-Krstev, Bojan Zlatković, Snežana Budimir, Branka Uzelac. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). Planta. 2019; 249 (5):1365-1377.
Chicago/Turabian StyleSvetlana Tošić; Dragana Stojičić; Violeta Slavkovska; Tatjana Mihailov-Krstev; Bojan Zlatković; Snežana Budimir; Branka Uzelac. 2019. "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)." Planta 249, no. 5: 1365-1377.
Bosnian pine (Pinus heldreichii Christ.) is a Tertiary relict subendemic to the mountains of Balkan Peninsula and a small area in southern Italy.
Dragana Stojičić; Branka Uzelac; Snežana Budimir. Bosnian Pine Pinus heldreichii Christ. Biosafety of Forest Transgenic Trees 2018, 49 -62.
AMA StyleDragana Stojičić, Branka Uzelac, Snežana Budimir. Bosnian Pine Pinus heldreichii Christ. Biosafety of Forest Transgenic Trees. 2018; ():49-62.
Chicago/Turabian StyleDragana Stojičić; Branka Uzelac; Snežana Budimir. 2018. "Bosnian Pine Pinus heldreichii Christ." Biosafety of Forest Transgenic Trees , no. : 49-62.
Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.
Branka Uzelac; Dusica Janosevic; Snezana Budimir. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. Advanced Structural Safety Studies 2018, 267 -282.
AMA StyleBranka Uzelac, Dusica Janosevic, Snezana Budimir. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. Advanced Structural Safety Studies. 2018; ():267-282.
Chicago/Turabian StyleBranka Uzelac; Dusica Janosevic; Snezana Budimir. 2018. "In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay." Advanced Structural Safety Studies , no. : 267-282.
We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.
Vojin Tadić; Marija Petrić; Branka Uzelac; Snežana Milošević; Zoran Vujčić; Jasmina Stevanović; Jovan Tadić. Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. Scientia Horticulturae 2018, 231, 210 -218.
AMA StyleVojin Tadić, Marija Petrić, Branka Uzelac, Snežana Milošević, Zoran Vujčić, Jasmina Stevanović, Jovan Tadić. Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. Scientia Horticulturae. 2018; 231 ():210-218.
Chicago/Turabian StyleVojin Tadić; Marija Petrić; Branka Uzelac; Snežana Milošević; Zoran Vujčić; Jasmina Stevanović; Jovan Tadić. 2018. "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1." Scientia Horticulturae 231, no. : 210-218.
The effect of abiotic (salicylic acid, jasmonic acid and methyl jasmonate) and biotic (chitosan and yeast extract) elicitors on the growth and xanthone accumulation in two hairy root clones of Gentiana dinarica Beck. was studied. The obtained results showed that clone 3 was more responsive to elicitor treatment than clone D. The production of dominant xanthone norswertianin-1-O-primeveroside was not significantly affected by either of the abiotic elicitor tested but was stimulated with chitosan treatment. The highest concentrations of all elicitors strongly increased the content of xanthone aglycone norswertianin, but simultaneously reducing the production of its glycoside norswertianin-1-O-primeveroside. The most efficient in enhancing norswertianin production was a 7-day treatment with salicylic acid (200 μM) and chitosan (50 mg l− 1), which yielded a 7.7- and a 24-fold increase in norswertianin content, respectively. In addition, treatment with biotic elicitors caused the occurrence of new xanthone compounds that were not detected in other samples. Free radical scavenging activity of xanthones was carried out by DPPH assay, and norswertianin showed the strongest activity.
Dijana Krstić-Milošević; Teodora Janković; Branka Uzelac; Dragan Vinterhalter; Branka Vinterhalter. Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica. Plant Cell, Tissue and Organ Culture (PCTOC) 2017, 130, 631 -640.
AMA StyleDijana Krstić-Milošević, Teodora Janković, Branka Uzelac, Dragan Vinterhalter, Branka Vinterhalter. Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica. Plant Cell, Tissue and Organ Culture (PCTOC). 2017; 130 (3):631-640.
Chicago/Turabian StyleDijana Krstić-Milošević; Teodora Janković; Branka Uzelac; Dragan Vinterhalter; Branka Vinterhalter. 2017. "Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica." Plant Cell, Tissue and Organ Culture (PCTOC) 130, no. 3: 631-640.
The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum. Initial changes in nuclear and plastidial organization were observed at a more advanced secretory stage, marking the onset of senescence. During trichome senescence, gradual reduction of the cytoplasm density occurred along with structural changes of the plastids and the tonoplast. As a result of inward blebbing of the cytoplasm into the vacuole, membrane bound vesicular structures appeared in the vacuolar space. At the late secretory stage, marked by an increase in vacuolation and extraplasmic space, degenerative changes included further fragmentation of the cytoplasm and deterioration of the tonoplast. Multimembrane myelin bodies observed in the vacuolar space were indicative of membrane digestion although plasma membrane did not appear massively degraded.
Branka Uzelac; Dušica Janošević; Dragana Stojičić; Snežana Budimir. Morphogenesis and developmental ultrastructure ofNicotiana tabacumshort glandular trichomes. Microscopy Research and Technique 2017, 80, 779 -786.
AMA StyleBranka Uzelac, Dušica Janošević, Dragana Stojičić, Snežana Budimir. Morphogenesis and developmental ultrastructure ofNicotiana tabacumshort glandular trichomes. Microscopy Research and Technique. 2017; 80 (7):779-786.
Chicago/Turabian StyleBranka Uzelac; Dušica Janošević; Dragana Stojičić; Snežana Budimir. 2017. "Morphogenesis and developmental ultrastructure ofNicotiana tabacumshort glandular trichomes." Microscopy Research and Technique 80, no. 7: 779-786.
Main conclusion In vitro conditions and benzyladenine influenced both content and composition of micropropagated Micromeria pulegium essential oils, with pulegone and menthone being the main essential oil components. The content and chemical composition of Micromeria pulegium (Rochel) Benth. essential oils were studied in native plant material at vegetative stage and in micropropagated plants, obtained from nodal segments cultured on solid MS medium supplemented with N6–benzyladenine (BA) or kinetin at different concentrations, alone or in combination with indole-3-acetic acid. Shoot proliferation was achieved in all treatments, but the highest biomass production was obtained after treatment with 10 μM BA. Phytochemical analysis identified up to 21 compounds in the essential oils of wild-growing and in vitro cultivated plants, both showing very high percentages of total monoterpenoids dominated by oxygenated monoterpenes of the menthane type. Pulegone and menthone were the main essential oil components detected in both wild-growing plants (60.07 and 26.85 %, respectively) and micropropagated plants grown on either plant growth regulator-free medium (44.57 and 29.14 %, respectively) or BA-supplemented medium (50.77 and 14.45 %, respectively). The percentage of total sesquiterpenoids increased in vitro, particularly owing to sesquiterpene hydrocarbons that were not found in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties. In addition, the morphology and structure of M. pulegium glandular trichomes in relation to the secretory process were characterized for the first time using SEM and light microscopy, and their secretion was histochemically analyzed.
Dragana Stojičić; Svetlana Tošić; Violeta Slavkovska; Bojan Zlatković; Snežana Budimir; Dušica Janošević; Branka Uzelac. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). Planta 2016, 244, 393 -404.
AMA StyleDragana Stojičić, Svetlana Tošić, Violeta Slavkovska, Bojan Zlatković, Snežana Budimir, Dušica Janošević, Branka Uzelac. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). Planta. 2016; 244 (2):393-404.
Chicago/Turabian StyleDragana Stojičić; Svetlana Tošić; Violeta Slavkovska; Bojan Zlatković; Snežana Budimir; Dušica Janošević; Branka Uzelac. 2016. "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)." Planta 244, no. 2: 393-404.
Branka Vinterhalter; Nevena Mitić; Dragan Vinterhalter; Branka Uzelac; Dijana Krstić-Milošević. Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa. Biologia 2016, 71, 1 .
AMA StyleBranka Vinterhalter, Nevena Mitić, Dragan Vinterhalter, Branka Uzelac, Dijana Krstić-Milošević. Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa. Biologia. 2016; 71 (2):1.
Chicago/Turabian StyleBranka Vinterhalter; Nevena Mitić; Dragan Vinterhalter; Branka Uzelac; Dijana Krstić-Milošević. 2016. "Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa." Biologia 71, no. 2: 1.
Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.
Branka Uzelac; Dušica Janošević; Ana Simonović; Václav Motyka; Petre I. Dobrev; Snežana Budimir. Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro. Protoplasma 2015, 253, 259 -275.
AMA StyleBranka Uzelac, Dušica Janošević, Ana Simonović, Václav Motyka, Petre I. Dobrev, Snežana Budimir. Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro. Protoplasma. 2015; 253 (2):259-275.
Chicago/Turabian StyleBranka Uzelac; Dušica Janošević; Ana Simonović; Václav Motyka; Petre I. Dobrev; Snežana Budimir. 2015. "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro." Protoplasma 253, no. 2: 259-275.
Branka Uzelac. Characterisation of programmed cell death and senescence in leaves of Nicotiana tabacum L. in vitro. Characterisation of programmed cell death and senescence in leaves of Nicotiana tabacum L. in vitro 2013, 1 .
AMA StyleBranka Uzelac. Characterisation of programmed cell death and senescence in leaves of Nicotiana tabacum L. in vitro. Characterisation of programmed cell death and senescence in leaves of Nicotiana tabacum L. in vitro. 2013; ():1.
Chicago/Turabian StyleBranka Uzelac. 2013. "Characterisation of programmed cell death and senescence in leaves of Nicotiana tabacum L. in vitro." Characterisation of programmed cell death and senescence in leaves of Nicotiana tabacum L. in vitro , no. : 1.
In Pinus peuce zygotic embryo culture grown on Gresshoff and Doy (1972; GD) basal medium, 2.22 μM benzyladenine (BA) was superior in promoting adventitious bud induction during 4 weeks comparing to kinetin or BA + kinetin. Shoot elongation was achieved on half-strength GD medium devoid of plant growth regulators and containing activated charcoal. Pulse treatment with 1 mM indole-3-butyric acid (IBA) for 2 h, followed by transfer to half-strength GD medium, produced the most efficient rooting. Rooted shoots were transplanted to the greenhouse and plantlets continued to grow and developed into phenotypically normal plants. Up to 10 plants per explant can be obtained within 36 weeks from culture initiation.
D. Stojičić; Dušica Janošević; B. Uzelac; V. Čokeša; S. Budimir. Micropropagation of Pinus peuce. Biologia plantarum 2012, 1 -3.
AMA StyleD. Stojičić, Dušica Janošević, B. Uzelac, V. Čokeša, S. Budimir. Micropropagation of Pinus peuce. Biologia plantarum. 2012; ():1-3.
Chicago/Turabian StyleD. Stojičić; Dušica Janošević; B. Uzelac; V. Čokeša; S. Budimir. 2012. "Micropropagation of Pinus peuce." Biologia plantarum , no. : 1-3.
Cytokinins are involved in plant cell proliferation leading to plant growth and morphogenesis. The size, activity and maintenance of the shoot apical meristem (SAM) are defined by a balanced rate of mitotic cell divisions and functional cell differentiation that are controlled by cytokinins. In order to investigate the effect of exogenous cytokinin on SAM, morpho-anatomical changes in the shoot apices of tobacco treated with benzyladenine (BA) were compared to those of untreated control plants.
Branka Uzelac; Dusica Janosevic; Dragana Stojicic; Snezana Budimir. Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. Archives of Biological Sciences 2012, 64, 511 -516.
AMA StyleBranka Uzelac, Dusica Janosevic, Dragana Stojicic, Snezana Budimir. Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. Archives of Biological Sciences. 2012; 64 (2):511-516.
Chicago/Turabian StyleBranka Uzelac; Dusica Janosevic; Dragana Stojicic; Snezana Budimir. 2012. "Effect of cytokinins on shoot apical meristem in Nicotiana tabacum." Archives of Biological Sciences 64, no. 2: 511-516.
This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.
Dragana Stojicic; Dusica Janosevic; Branka Uzelac; V. Cokesa; Snezana Budimir. In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. Archives of Biological Sciences 2012, 64, 503 -509.
AMA StyleDragana Stojicic, Dusica Janosevic, Branka Uzelac, V. Cokesa, Snezana Budimir. In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. Archives of Biological Sciences. 2012; 64 (2):503-509.
Chicago/Turabian StyleDragana Stojicic; Dusica Janosevic; Branka Uzelac; V. Cokesa; Snezana Budimir. 2012. "In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth." Archives of Biological Sciences 64, no. 2: 503-509.