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M. Trifunović
Department of Plant Physiology, Institute for Biological Research “Siniša Stanković“—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia

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Book chapter
Published: 05 May 2021 in Plant Breeding - Current and Future Views
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The production and breeding of plants with desired properties are possible by a fundamental biotechnological technique, genetic engineering. Applying and developing of genetic engineering procedures also enable preservation and improvement of plant species endangered in nature, including medicinal plant common centaury (Centaurium erythraea Rafn.). Numerous developmental processes in plants are controlled by cytokinins (CKs). The only so far known enzyme involved in CK catabolism is cytokinin oxidase/dehydrogenase (CKX). Genes coding for two Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were successfully introduced into centaury root explants. Subsequently, the contents of endogenous CKs in AtCKX-overexpressing centaury plants grown in vitro were investigated. Simultaneous secondary metabolite analyses showed antibacterial and antifungal activity of transgenic centaury plants and suggested their use as potential producers of anti-cancer compounds. Considering that centaury can inhabit saline soils in natural habitats, following investigations included evaluation of salinity tolerance in vitro. All obtained and summarized results indicated that transgenic AtCKX centaury plants could serve as a suitable model for studies of numerous physiological and developmental processes under endogenous phytohormonal control.

ACS Style

Milana Trifunović-Momčilov; Václav Motyka. Transgenic AtCKX Centaury Plants Grown In Vitro. Plant Breeding - Current and Future Views 2021, 1 .

AMA Style

Milana Trifunović-Momčilov, Václav Motyka. Transgenic AtCKX Centaury Plants Grown In Vitro. Plant Breeding - Current and Future Views. 2021; ():1.

Chicago/Turabian Style

Milana Trifunović-Momčilov; Václav Motyka. 2021. "Transgenic AtCKX Centaury Plants Grown In Vitro." Plant Breeding - Current and Future Views , no. : 1.

Review
Published: 30 April 2021 in Plants
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In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.

ACS Style

Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Slađana Jevremović; Angelina Subotić. Bulb Dormancy In Vitro—Fritillaria meleagris: Initiation, Release and Physiological Parameters. Plants 2021, 10, 902 .

AMA Style

Marija Marković, Milana Trifunović Momčilov, Branka Uzelac, Slađana Jevremović, Angelina Subotić. Bulb Dormancy In Vitro—Fritillaria meleagris: Initiation, Release and Physiological Parameters. Plants. 2021; 10 (5):902.

Chicago/Turabian Style

Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Slađana Jevremović; Angelina Subotić. 2021. "Bulb Dormancy In Vitro—Fritillaria meleagris: Initiation, Release and Physiological Parameters." Plants 10, no. 5: 902.

Journal article
Published: 14 January 2021 in Plants
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Aquaporins comprise a large group of transmembrane proteins responsible for water transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins, nothing is known about this protein family in Impatiens walleriana, a commercially important horticultural plant, which is sensitive to drought stress. In the present study, attention is given to the molecular characterization of aquaporins in I. walleriana and their expression during drought stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein. Drought stress and recovery treatment affected the aquaporins expression in I. walleriana leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively. Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins expanded knowledge about plant aquaporins, while its expression during drought and recovery contributed to I. walleriana drought tolerance mechanisms and re-acclimation.

ACS Style

Marija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milan Dragićević; Ana Simonović; Snežana Milošević. Molecular Characterization and Expression of Four Aquaporin Genes in Impatiens walleriana During Drought Stress and Recovery. Plants 2021, 10, 154 .

AMA Style

Marija Đurić, Angelina Subotić, Ljiljana Prokić, Milana Trifunović-Momčilov, Aleksandar Cingel, Milan Dragićević, Ana Simonović, Snežana Milošević. Molecular Characterization and Expression of Four Aquaporin Genes in Impatiens walleriana During Drought Stress and Recovery. Plants. 2021; 10 (1):154.

Chicago/Turabian Style

Marija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milan Dragićević; Ana Simonović; Snežana Milošević. 2021. "Molecular Characterization and Expression of Four Aquaporin Genes in Impatiens walleriana During Drought Stress and Recovery." Plants 10, no. 1: 154.

Review
Published: 31 December 2020 in Plants
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Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic embryos. When SE is initiated from centaury root explants, the process occurs spontaneously (on hormone-free medium), directly (without the callusing phase), and the somatic embryos are of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is indirect (preceded by callusing). Histological observations and culture conditions are compared in these two systems. The changes in antioxidative enzymes were followed during SE from the leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were analyzed using a variety of approaches. The newest and preliminary results, including centaury transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed as perspectives of centaury research.

ACS Style

Ana D. Simonović; Milana M. Trifunović-Momčilov; Biljana K. Filipović; Marija P. Marković; Milica D. Bogdanović; Angelina R. Subotić. Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review. Plants 2020, 10, 70 .

AMA Style

Ana D. Simonović, Milana M. Trifunović-Momčilov, Biljana K. Filipović, Marija P. Marković, Milica D. Bogdanović, Angelina R. Subotić. Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review. Plants. 2020; 10 (1):70.

Chicago/Turabian Style

Ana D. Simonović; Milana M. Trifunović-Momčilov; Biljana K. Filipović; Marija P. Marković; Milica D. Bogdanović; Angelina R. Subotić. 2020. "Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review." Plants 10, no. 1: 70.

Journal article
Published: 13 November 2020 in Plants
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This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.

ACS Style

Marija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milorad Vujičić; Snežana Milošević. Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. Plants 2020, 9, 1559 .

AMA Style

Marija Đurić, Angelina Subotić, Ljiljana Prokić, Milana Trifunović-Momčilov, Aleksandar Cingel, Milorad Vujičić, Snežana Milošević. Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. Plants. 2020; 9 (11):1559.

Chicago/Turabian Style

Marija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milorad Vujičić; Snežana Milošević. 2020. "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro." Plants 9, no. 11: 1559.

Journal article
Published: 13 November 2020 in Plants
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The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.

ACS Style

Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Olga Radulović; Snežana Milošević; Slađana Jevremović; Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. Plants 2020, 9, 1573 .

AMA Style

Marija Marković, Milana Trifunović Momčilov, Branka Uzelac, Olga Radulović, Snežana Milošević, Slađana Jevremović, Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. Plants. 2020; 9 (11):1573.

Chicago/Turabian Style

Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Olga Radulović; Snežana Milošević; Slađana Jevremović; Angelina Subotić. 2020. "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs." Plants 9, no. 11: 1573.

Journal article
Published: 27 October 2020 in Plants
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Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors—ancymidol (A) and paclobutrazol (P)—the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb’s dormancy and its sprouting.

ACS Style

Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Aleksandar Cingel; Snežana Milošević; Slađana Jevremović; Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content. Plants 2020, 9, 1449 .

AMA Style

Marija Marković, Milana Trifunović Momčilov, Branka Uzelac, Aleksandar Cingel, Snežana Milošević, Slađana Jevremović, Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content. Plants. 2020; 9 (11):1449.

Chicago/Turabian Style

Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Aleksandar Cingel; Snežana Milošević; Slađana Jevremović; Angelina Subotić. 2020. "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content." Plants 9, no. 11: 1449.

Journal article
Published: 08 May 2020 in Plants
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The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.

ACS Style

Olga Radulović; Slaviša Stanković; Branka Uzelac; Vojin Tadić; Milana Trifunović-Momčilov; Jelena Lozo; Marija Marković. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. Plants 2020, 9, 599 .

AMA Style

Olga Radulović, Slaviša Stanković, Branka Uzelac, Vojin Tadić, Milana Trifunović-Momčilov, Jelena Lozo, Marija Marković. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. Plants. 2020; 9 (5):599.

Chicago/Turabian Style

Olga Radulović; Slaviša Stanković; Branka Uzelac; Vojin Tadić; Milana Trifunović-Momčilov; Jelena Lozo; Marija Marković. 2020. "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations." Plants 9, no. 5: 599.

Research article
Published: 13 April 2020 in Annals of Applied Biology
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ACS Style

Milana Trifunović-Momčilov; Danijela Paunović; Snežana Milošević; Marija Marković; Slađana Jevremović; Ivana Dragićević; Angelina Subotić. Salinity stress response of non‐transformed and AtCKX transgenic centaury ( Centaurium erythraea Rafn.) shoots and roots grown in vitro. Annals of Applied Biology 2020, 177, 74 -89.

AMA Style

Milana Trifunović-Momčilov, Danijela Paunović, Snežana Milošević, Marija Marković, Slađana Jevremović, Ivana Dragićević, Angelina Subotić. Salinity stress response of non‐transformed and AtCKX transgenic centaury ( Centaurium erythraea Rafn.) shoots and roots grown in vitro. Annals of Applied Biology. 2020; 177 (1):74-89.

Chicago/Turabian Style

Milana Trifunović-Momčilov; Danijela Paunović; Snežana Milošević; Marija Marković; Slađana Jevremović; Ivana Dragićević; Angelina Subotić. 2020. "Salinity stress response of non‐transformed and AtCKX transgenic centaury ( Centaurium erythraea Rafn.) shoots and roots grown in vitro." Annals of Applied Biology 177, no. 1: 74-89.

Journal article
Published: 01 January 2019 in Environmental Engineering and Management Journal
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ACS Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snezana Trifunovic; Ana Ciric; Jasmina Glamočlija; Sladana Jevremovic; Angelina Subotic. ANTIMICROBIAL ACTIVITY, ANTIOXIDANT POTENTIAL AND TOTAL PHENOLIC CONTENT OF TRANSGENIC ATCKX1 CENTAURY (Centaurium erythraea Rafn.) PLANTS GROWN IN VITRO. Environmental Engineering and Management Journal 2019, 18, 2063 -2072.

AMA Style

Milana Trifunović-Momčilov, Dijana Krstić-Milošević, Snezana Trifunovic, Ana Ciric, Jasmina Glamočlija, Sladana Jevremovic, Angelina Subotic. ANTIMICROBIAL ACTIVITY, ANTIOXIDANT POTENTIAL AND TOTAL PHENOLIC CONTENT OF TRANSGENIC ATCKX1 CENTAURY (Centaurium erythraea Rafn.) PLANTS GROWN IN VITRO. Environmental Engineering and Management Journal. 2019; 18 (9):2063-2072.

Chicago/Turabian Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snezana Trifunovic; Ana Ciric; Jasmina Glamočlija; Sladana Jevremovic; Angelina Subotic. 2019. "ANTIMICROBIAL ACTIVITY, ANTIOXIDANT POTENTIAL AND TOTAL PHENOLIC CONTENT OF TRANSGENIC ATCKX1 CENTAURY (Centaurium erythraea Rafn.) PLANTS GROWN IN VITRO." Environmental Engineering and Management Journal 18, no. 9: 2063-2072.

Book chapter
Published: 09 May 2017 in Reference Series in Phytochemistry
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The genus Centaurium includes about 50 plant species found throughout the northern hemisphere. Plant species Centaurium erythraea Rafn., commonly known as common centaury, has been used for centuries for medical purposes. Centaury is used to treat anemia, jaundice, and gout and to cure febrile conditions and regulate blood sugar. So far, centaury species were genetically transformed mostly using Agrobacterium rhizogenes. Only one report has described A. tumefaciens-mediated gene delivery for the production of transgenic centaury plants. Genetic transformation of centaury using AtCKX genes did not influence the quality but influenced the quantity of xanthones in shoots and roots. The majority of AtCKX transformed centaury lines grown in vitro produced increased eustomin and/or demethyleustomin content than untransformed control plants. This work clearly demonstrates, for the first time, the effect of centaury secoiridoids and xanthones on colorectal cancer cell line (DLD1) and its resistant counterpart (DLD1-TxR). The xanthone eustomin showed the most significant cell growth inhibition effects. Since xanthones are increasingly being used for their pharmacological properties, AtCKX transgenic centaury plants could be used as a useful source of plant material for the production of novel drugs.

ACS Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snežana Trifunović; Ana Podolski-Renić; Milica Pešić; Angelina Subotić. Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds. Reference Series in Phytochemistry 2017, 205 -230.

AMA Style

Milana Trifunović-Momčilov, Dijana Krstić-Milošević, Snežana Trifunović, Ana Podolski-Renić, Milica Pešić, Angelina Subotić. Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds. Reference Series in Phytochemistry. 2017; ():205-230.

Chicago/Turabian Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snežana Trifunović; Ana Podolski-Renić; Milica Pešić; Angelina Subotić. 2017. "Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds." Reference Series in Phytochemistry , no. : 205-230.

Journal article
Published: 01 January 2017 in Archives of Biological Sciences
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The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.

ACS Style

Marija Petric; Angelina Subotic; Sladjana Jevremovic; Milana Trifunovic-Momcilov; Vojin Tadic; Marica Grujic; Zoran Vujcic. Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. Archives of Biological Sciences 2017, 69, 619 -625.

AMA Style

Marija Petric, Angelina Subotic, Sladjana Jevremovic, Milana Trifunovic-Momcilov, Vojin Tadic, Marica Grujic, Zoran Vujcic. Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. Archives of Biological Sciences. 2017; 69 (4):619-625.

Chicago/Turabian Style

Marija Petric; Angelina Subotic; Sladjana Jevremovic; Milana Trifunovic-Momcilov; Vojin Tadic; Marica Grujic; Zoran Vujcic. 2017. "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base." Archives of Biological Sciences 69, no. 4: 619-625.

Book chapter
Published: 23 November 2016 in Reference Series in Phytochemistry
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The genus Centaurium includes about 50 plant species found throughout the northern hemisphere. Plant species Centaurium erythraea Rafn., commonly known as common centaury, has been used for centuries for medical purposes. Centaury is used to treat anemia, jaundice, and gout and to cure febrile conditions and regulate blood sugar. So far, centaury species were genetically transformed mostly using Agrobacterium rhizogenes. Only one report has described A. tumefaciens-mediated gene delivery for the production of transgenic centaury plants. Genetic transformation of centaury using AtCKX genes did not influence the quality but influenced the quantity of xanthones in shoots and roots. The majority of AtCKX transformed centaury lines grown in vitro produced increased eustomin and/or demethyleustomin content than untransformed control plants. This work clearly demonstrates, for the first time, the effect of centaury secoiridoids and xanthones on colorectal cancer cell line (DLD1) and its resistant counterpart (DLD1-TxR). The xanthone eustomin showed the most significant cell growth inhibition effects. Since xanthones are increasingly being used for their pharmacological properties, AtCKX transgenic centaury plants could be used as a useful source of plant material for the production of novel drugs.

ACS Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snežana Trifunović; Ana Podolski-Renić; Milica Pešić; Angelina Subotić. Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds. Reference Series in Phytochemistry 2016, 1 -26.

AMA Style

Milana Trifunović-Momčilov, Dijana Krstić-Milošević, Snežana Trifunović, Ana Podolski-Renić, Milica Pešić, Angelina Subotić. Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds. Reference Series in Phytochemistry. 2016; ():1-26.

Chicago/Turabian Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snežana Trifunović; Ana Podolski-Renić; Milica Pešić; Angelina Subotić. 2016. "Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds." Reference Series in Phytochemistry , no. : 1-26.

Book chapter
Published: 22 July 2016 in Reference Series in Phytochemistry
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The genus Centaurium includes about 50 plant species found throughout the northern hemisphere. Plant species Centaurium erythraea Rafn., commonly known as common centaury, has been used for centuries for medical purposes. Centaury is used to treat anemia, jaundice, and gout and to cure febrile conditions and regulate blood sugar. So far, centaury species were genetically transformed mostly using Agrobacterium rhizogenes. Only one report has described A. tumefaciens-mediated gene delivery for the production of transgenic centaury plants. Genetic transformation of centaury using AtCKX genes did not influence the quality but influenced the quantity of xanthones in shoots and roots. The majority of AtCKX transformed centaury lines grown in vitro produced increased eustomin and/or demethyleustomin content than untransformed control plants. This work clearly demonstrates, for the first time, the effect of centaury secoiridoids and xanthones on colorectal cancer cell line (DLD1) and its resistant counterpart (DLD1-TxR). The xanthone eustomin showed the most significant cell growth inhibition effects. Since xanthones are increasingly being used for their pharmacological properties, AtCKX transgenic centaury plants could be used as a useful source of plant material for the production of novel drugs.

ACS Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snežana Trifunović; Ana Podolski-Renić; Milica Pešić; Angelina Subotić. Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds. Reference Series in Phytochemistry 2016, 1 -26.

AMA Style

Milana Trifunović-Momčilov, Dijana Krstić-Milošević, Snežana Trifunović, Ana Podolski-Renić, Milica Pešić, Angelina Subotić. Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds. Reference Series in Phytochemistry. 2016; ():1-26.

Chicago/Turabian Style

Milana Trifunović-Momčilov; Dijana Krstić-Milošević; Snežana Trifunović; Ana Podolski-Renić; Milica Pešić; Angelina Subotić. 2016. "Secondary Metabolite Profile of Transgenic Centaury (Centaurium erythraea Rafn.) Plants, Potential Producers of Anticancer Compounds." Reference Series in Phytochemistry , no. : 1-26.

Journal article
Published: 01 July 2016 in South African Journal of Botany
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Dragana Antonic; Snežana Milošević; Aleksandar Cingel; M. Lojić; M. Trifunović-Momčilov; M. Petric; Angelina Subotić; Ana Simonović. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. South African Journal of Botany 2016, 105, 226 -233.

AMA Style

Dragana Antonic, Snežana Milošević, Aleksandar Cingel, M. Lojić, M. Trifunović-Momčilov, M. Petric, Angelina Subotić, Ana Simonović. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. South African Journal of Botany. 2016; 105 ():226-233.

Chicago/Turabian Style

Dragana Antonic; Snežana Milošević; Aleksandar Cingel; M. Lojić; M. Trifunović-Momčilov; M. Petric; Angelina Subotić; Ana Simonović. 2016. "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought." South African Journal of Botany 105, no. : 226-233.

Journal article
Published: 01 January 2016 in Archives of Biological Sciences
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Over 20% of all protein domains are currently annotated as ?domains of unknown function? or DUFs. In a recently identified Centaurium erythraea arabinogalactan peptide, CeAGP3 (AGN92423), a conserved DUF1070 domain was found. Since identifying functions for DUFs is important in systems biology, we have analyzed the distribution and structure of DUF1070 domain (pfam06376) using a set of bioinformatics tools. There are 271 publically available DUF1070 members from 25 diverse families of vascular plants, and most are short sequences (50-100 aa). The N-terminal signal peptide (Nsp) was found in almost all complete sequences. In 233 sequences, at least two noncontiguous prolines were found as clustered dipeptides predicted to be hydroxylated and glycosylated with type II arabino-3,6-galactans, thus representing AG-II glycomodules. In addition, 35 sequences contained a region rich in basic residues (basic linker, BL). The N-terminal part of the DUF1070 domain is comprised of (part of) AG-II and/or BL, while the highly conserved C-terminus is a region of 26 aa, termed SH26. In 212 sequences, SH26 was a typical glycosylphosphatidylinositol lipid anchor signal peptide (GPIsp), but in 83 cases GPIsp was not predicted due to software constraints. In sequences where both Nsp and GPIsp were predicted, the length of mature peptides could be calculated, and it was 10-16 aa. Our analysis suggests that DUF1070 members are arabinogalactan (AG) peptides, of which the majority are GPI-anchored. DUF1070 is the only conserved domain found in classical arabinogalactan proteins and AG peptides. The SH26 region can be used for mining and annotation of AG peptides.

ACS Style

Ana D. Simonović; Milan B. Dragićević; Milica D. Bogdanović; Milana M. Trifunović-Momčilov; Angelina R. Subotić; Slađana Todorović. DUF1070 as a signature domain of a subclass of arabinogalactan peptides. Archives of Biological Sciences 2016, 68, 737 -746.

AMA Style

Ana D. Simonović, Milan B. Dragićević, Milica D. Bogdanović, Milana M. Trifunović-Momčilov, Angelina R. Subotić, Slađana Todorović. DUF1070 as a signature domain of a subclass of arabinogalactan peptides. Archives of Biological Sciences. 2016; 68 (4):737-746.

Chicago/Turabian Style

Ana D. Simonović; Milan B. Dragićević; Milica D. Bogdanović; Milana M. Trifunović-Momčilov; Angelina R. Subotić; Slađana Todorović. 2016. "DUF1070 as a signature domain of a subclass of arabinogalactan peptides." Archives of Biological Sciences 68, no. 4: 737-746.

Journal article
Published: 01 December 2015 in Comptes Rendus. Biologies
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Morphogenesis in vitro is a complex and still poorly defined process. We investigated esterase and peroxidase isoforms detected in bulb scale, during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4 °C or on a medium with an increased concentration of sucrose (4.5%) for 30 days. After these pre-treatments, the bulb scales were further grown on nutrient media that contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred at the same explant. The highest numbers of somatic embryos and bulblets were regenerated on the medium containing 2,4-D and KIN (1mg/L each), while morphogenesis was most successful at a TDZ concentration between 0.5 and 1mg/L. Monitoring of esterases and peroxidases was performed by growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1mg/L), and the number and activity of isoforms were followed every 7 days for 4 weeks. In control explants, six isoforms of esterase were observed. Three isoforms of peroxidase were not detected in the control bulb scale, which has not begun its morphogenesis process.

ACS Style

Marija Petrić; Angelina Subotić; Sladjana Jevremović; Milana Trifunović-Momčilov; Vojin Tadić; Marica Grujić; Zoran Vujčić. Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture. Comptes Rendus. Biologies 2015, 338, 793 -802.

AMA Style

Marija Petrić, Angelina Subotić, Sladjana Jevremović, Milana Trifunović-Momčilov, Vojin Tadić, Marica Grujić, Zoran Vujčić. Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture. Comptes Rendus. Biologies. 2015; 338 (12):793-802.

Chicago/Turabian Style

Marija Petrić; Angelina Subotić; Sladjana Jevremović; Milana Trifunović-Momčilov; Vojin Tadić; Marica Grujić; Zoran Vujčić. 2015. "Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture." Comptes Rendus. Biologies 338, no. 12: 793-802.

Journal article
Published: 20 November 2015 in Journal of Plant Growth Regulation
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Phytohormones are important regulators of numerous developmental and physiological processes in plants. Spontaneous morphogenesis of the common centaury (Centaurium erythraea Rafn.) is possible on nutrition medium without addition of any plant growth regulator depending solely on endogenous phytohormone levels. Thus, this plant species represents a very good model system for the investigation of numerous physiological processes under phytohormonal control in vitro. We analysed the total amount of endogenous cytokinins (CKs) including the contents of their individual groups in shoots and roots of C. erythraea plants grown in vitro. The total amount of endogenous CKs was 1.4 times higher in shoots than in roots. Inactive or weakly active N-glucosides found to predominate in both organs of centaury plants, whereas free bases and O-glucosides represented only a small portion of the total CK pool. Consequently, centaury roots showed higher IAA content as well as IAA/free CK base ratios compared to shoots. Centaury tissues also showed increased levels of “stress hormones”. In contrast to SA, considerably higher levels of ABA were found in centaury shoots than in roots. Our results could serve as a basis for understanding and elucidating spontaneous de novo shoot organogenesis and further plant regeneration of C. erythraea in vitro.

ACS Style

Milana Trifunović-Momčilov; Václav Motyka; Ivana Dragićević; Marija Petrić; Sladjana Jevremović; Jiří Malbeck; Josef Holík; Petre I. Dobrev; Angelina Subotić. Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. Journal of Plant Growth Regulation 2015, 35, 543 -552.

AMA Style

Milana Trifunović-Momčilov, Václav Motyka, Ivana Dragićević, Marija Petrić, Sladjana Jevremović, Jiří Malbeck, Josef Holík, Petre I. Dobrev, Angelina Subotić. Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. Journal of Plant Growth Regulation. 2015; 35 (2):543-552.

Chicago/Turabian Style

Milana Trifunović-Momčilov; Václav Motyka; Ivana Dragićević; Marija Petrić; Sladjana Jevremović; Jiří Malbeck; Josef Holík; Petre I. Dobrev; Angelina Subotić. 2015. "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro." Journal of Plant Growth Regulation 35, no. 2: 543-552.

Book chapter
Published: 18 June 2015 in The Gentianaceae - Volume 2: Biotechnology and Applications
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Histochemical localization using the β-Glc Yariv reagent and immunolocalization with arabinogalactan protein (AGP) reactive antibodies (LM2, JIM13, JIM15, JIM16, MAC207) were performed during morphogenic induction in root cultures of Centaurium erythraea Rafn cultured on half-strength MS medium without plant growth regulators. The observations revealed that β-Glc Yariv reagent specifically bound to AGPs in cells of the root epidermis and central cylinder. Monoclonal antibodies recognizing AGPs were localized in epidermal cells and cells of the central cylinder (LM2 , JIM16), vascular tissue (JIM15), globular somatic embryos (LM2, MAC207), and de novo-formed meristematic centers in the root cortex (JIM16). The effect of β-Glc Yariv reagent was investigated after supplementation (0–75 μM) in the culture medium. The morphogenetic potential was increased at lower concentrations (15–25 μM) of treatment with β-Glc Yariv reagent but inhibited (40 %) at the highest concentration. These results implicate that AGPs play a significant role during the development of somatic embryos and adventitious shoots in root cultures of C. erythraea.

ACS Style

Milana Trifunović; Angelina Subotić; Marija Petrić; Sladjana Jevremović. The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro. The Gentianaceae - Volume 2: Biotechnology and Applications 2015, 113 -138.

AMA Style

Milana Trifunović, Angelina Subotić, Marija Petrić, Sladjana Jevremović. The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro. The Gentianaceae - Volume 2: Biotechnology and Applications. 2015; ():113-138.

Chicago/Turabian Style

Milana Trifunović; Angelina Subotić; Marija Petrić; Sladjana Jevremović. 2015. "The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro." The Gentianaceae - Volume 2: Biotechnology and Applications , no. : 113-138.

Journal article
Published: 07 March 2015 in Plant Cell, Tissue and Organ Culture (PCTOC)
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Arabinogalactan proteins (AGPs) are cell wall hydroxyproline-rich glycoproteins with diverse functions in plant growth and development. In this work the role of AGPs was comparatively studied in four developmental pathways that can be induced from centaury leaf explants—indirect somatic embryogenesis (ISE), indirect and direct shoot development (ISD and DSD) and direct root development (DRD). The addition of β-d-glucosyl Yariv (βGlcY) reagent, a synthetic phenylglycoside that specifically crosslinks AGPs, to the growth medium reduced the number of somatic embryos and adventitious buds formed per explant. The morphogenetic paths can be arranged in order of increasing sensitivity to βGlcY as: DRD (insensitive) CeAGP1–CeAGP4, of which CeAGP1, 2 and 4 code for fasciclin-like AGPs. CeAGP3 is an AG peptide with conserved DUF1070 domain. Expression pattern of these genes indicated specific involvement of CeAGP1 in ISE and unspecific involvement of CeAGP3 in morphogenesis.

ACS Style

Ana D. Simonović; Biljana K. Filipović; Milana M. Trifunović; Saša N. Malkov; Vedrana P. Milinkovic; Slađana B. Jevremović; Angelina R. Subotić. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 2: the role of arabinogalactan proteins. Plant Cell, Tissue and Organ Culture (PCTOC) 2015, 121, 721 -739.

AMA Style

Ana D. Simonović, Biljana K. Filipović, Milana M. Trifunović, Saša N. Malkov, Vedrana P. Milinkovic, Slađana B. Jevremović, Angelina R. Subotić. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 2: the role of arabinogalactan proteins. Plant Cell, Tissue and Organ Culture (PCTOC). 2015; 121 (3):721-739.

Chicago/Turabian Style

Ana D. Simonović; Biljana K. Filipović; Milana M. Trifunović; Saša N. Malkov; Vedrana P. Milinkovic; Slađana B. Jevremović; Angelina R. Subotić. 2015. "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 2: the role of arabinogalactan proteins." Plant Cell, Tissue and Organ Culture (PCTOC) 121, no. 3: 721-739.