This work aimed at investigating the interactive effects of salt-signaling molecules, i.e., ethylene, extracellular ATP (eATP), H₂O₂, and cytosolic Ca²⁺ ([Ca²⁺]_cyt), on the regulation of K⁺/Na⁺ homeostasis in Arabidopsisthaliana. The presence of eATP shortened Col-0 hypocotyl length under no-salt conditions. Moreover, eATP decreased relative electrolyte leakage and lengthened root length significantly in salt-treated Col-0 plants but had no obvious effects on the ethylene-insensitive mutants etr1-1 and ein3-1eil1-1. Steady-state ionic flux kinetics showed that exogenous 1-aminocyclopropane-1-carboxylic acid (ACC, an ethylene precursor) and eATP-Na₂ (an eATP donor) significantly increased Na⁺ extrusion and suppressed K⁺ loss during short-term NaCl treatment. Moreover, ACC remarkably raised the fluorescence intensity of salt-elicited H₂O₂ and cytosolic Ca²⁺. Our qPCR data revealed that during 12 h of NaCl stress, application of ACC increased the expression of AtSOS1 and AtAHA1, which encode the plasma membrane (PM) Na⁺/H⁺ antiporters (SOS1) and H⁺-ATPase (H⁺ pumps), respectively. In addition, eATP markedly increased the transcription of AtEIN3, AtEIL1, and AtETR1, and ACC treatment of Col-0 roots under NaCl stress conditions caused upregulation of AtRbohF and AtSOS2/3, which directly contribute to the H₂O₂ and Ca²⁺ signaling pathways, respectively. Briefly, ethylene was triggered by eATP, a novel upstream signaling component, which then activated and strengthened the H₂O₂ and Ca²⁺ signaling pathways to maintain K⁺/Na⁺ homeostasis under salinity.

In the leaves of most herbaceous plants, stomata exist in both the adaxial (upper) and abaxial (lower) surfaces^1,2. Many previous studies have reported that stomata on the abaxial surface are more responsive to light than those on the adaxial surface^{3,4,5,6,7,8,9}. However, the underlying molecular mechanism is still unclear. Here, by examining the model plants Arabidopsis and tobacco, we confirmed that the distinct feature occurred at the guard cell level. Next, with both single-cell RNA sequencing and transcriptome sequencing, we compared gene expression patterns of adaxial and abaxial guard cells and highlighted the possibility of different utilization of potassium ion (K⁺) channels. Via in silico OnGuard simulation and genetic modifications, we found that adaxial and abaxial guard cells rely on different K⁺_in channels, which control K⁺ influx for stomatal opening. The present study provides insights into understanding the distinct stomatal light response of different leaf surfaces.

Sulfur, widely present in the soil and atmosphere, is one of the essential elements for plants. Sulfate is a dominant form of sulfur in soils taken up by plant roots. In addition to the assimilation into sulfur compounds essential for plant growth and development, it has been reported recently that sulfate as well as other sulfur containing compounds can also induce stomatal movement. Here, we first summarized the uptake and transport of sulfate and atmospheric sulfur, including H₂O and SO₂, and then, focused on the effects of inorganic and organic sulfur on stomatal movement. We concluded all the transporters for different sulfur compounds, and compared the expression level of those transporters in guard cells and mesophyll cells. The relationship between abscisic acid and sulfur compounds in regulation of stomatal movement were also discussed.