Drought stress is a major environmental constraint for plant growth. Climate-change-driven increases in ambient temperatures resulted in reduced or unevenly distributed rainfalls, leading to increased soil drought. Carex duriuscula C. A. Mey is a typical drought-tolerant sedge, but few reports have examined the mechanisms conferring its tolerant traits. In the present study, the drought responses of C. duriuscula were assessed by quantifying activity of antioxidant enzymes in its leaf and root tissues and evaluating the relative contribution of organic and inorganic osmolyte in plant osmotic adjustment, linking it with the patterns of the ion acquisition by roots. Two levels of stress—mild (MD) and severe (SD) drought treatments—were used, followed by re-watering. Drought stress caused reduction in a relative water content and chlorophyll content of leaves; this was accompanied by an increase in the hydrogen peroxide (H₂O₂) and superoxide (O_2⁻) contents in leaves and roots. Under MD stress, the activities of catalase (CAT), peroxidase (POD), and glutathione peroxidase (GPX) increased in leaves, whereas, in roots, only CAT and POD activities increased. SD stress led to an increase in the activities of CAT, POD, superoxide dismutase (SOD), and GPX in both tissues. The levels of proline, soluble sugars, and soluble proteins in the leaves also increased. Under both MD and SD stress conditions, C. duriuscula increased K⁺, Na⁺, and Cl⁻ uptake by plant roots, which resulted in an increased K⁺, Na⁺, and Cl⁻ concentrations in leaves and roots. This reliance on inorganic osmolytes enables a cost-efficient osmotic adjustment in C. duriuscula. Overall, this study revealed that C. duriuscula was able to survive arid environments due to an efficient operation of its ROS-scavenging systems and osmotic adjustment mechanisms.

CDPKs (Ca²⁺-Dependent Protein Kinases) are very important regulators in plant response to abiotic stress. The molecular regulatory mechanism of CDPKs involved in salt stress tolerance remains unclear, although some CDPKs have been identified in salt-stress signaling. Here, we investigated the function of an Arabidopsis CDPK, CPK12, in salt-stress signaling. The CPK12-RNA interference (RNAi) mutant was much more sensitive to salt stress than the wild-type plant GL1 in terms of seedling growth. Under NaCl treatment, Na⁺ levels in the roots of CPK12-RNAi plants increased and were higher than levels in GL1 plants. In addition, the level of salt-elicited H₂O₂ production was higher in CPK12-RNAi mutants than in wild-type GL1 plants after NaCl treatment. Collectively, our results suggest that CPK12 is required for plant adaptation to salt stress.

Plant salt tolerance relies on a coordinated functioning of different tissues and organs. Salinity tissue tolerance is one of the key traits that confers plant adaptation to saline environment. This trait implies maintenance low cytosolic Na⁺/K⁺ ratio in metabolically active cellular compartments. In this study, we used Nitraria sibirica Pall., a perennial woody halophytes species, to understand the mechanistic basis of its salinity tissue tolerance. The results showed that the growth of seedlings was stimulated by 100-200 mM NaCl treatment. The ions distribution analysis showed that the leaves acted as Na⁺ sink while plant root possess superior K⁺ retention. The excessive Na⁺ absorbed from soil was mainly transported to the shoot and eventually sequestrated into mesophyll vacuoles in the leaves. As a result, N. sibirica could keep optimal balance of K⁺/Na⁺ at a tissue- and cell-specific level under saline condition. To enable this, N. sibirica increased both vacuolar H⁺-ATPase and H⁺-PPase enzymes activities and up-regulated expressions of NsVHA, NsVP1 and NsNHX1 genes. Vacuolar Na⁺ sequestration in the leaf mesophyll mediated by NsVHA, NsVP1 and NsNHX1 reduced the Na⁺ concentration in cytosol and inhibited further K⁺ loss. Meanwhile, N.sibirica enhanced the TPK expression at the transcriptional level to promote K⁺ efflux from vacuole into cytoplasm, assisting in maintaining cytosolic K⁺ homeostasis. It is concluded that the tissue tolerance traits such as vacuolar Na⁺ sequestration and intracellular K⁺ homeostasis is critical to confer adaptation of N. sibirica to soil salinity.

This work aimed at investigating the interactive effects of salt-signaling molecules, i.e., ethylene, extracellular ATP (eATP), H₂O₂, and cytosolic Ca²⁺ ([Ca²⁺]_cyt), on the regulation of K⁺/Na⁺ homeostasis in Arabidopsisthaliana. The presence of eATP shortened Col-0 hypocotyl length under no-salt conditions. Moreover, eATP decreased relative electrolyte leakage and lengthened root length significantly in salt-treated Col-0 plants but had no obvious effects on the ethylene-insensitive mutants etr1-1 and ein3-1eil1-1. Steady-state ionic flux kinetics showed that exogenous 1-aminocyclopropane-1-carboxylic acid (ACC, an ethylene precursor) and eATP-Na₂ (an eATP donor) significantly increased Na⁺ extrusion and suppressed K⁺ loss during short-term NaCl treatment. Moreover, ACC remarkably raised the fluorescence intensity of salt-elicited H₂O₂ and cytosolic Ca²⁺. Our qPCR data revealed that during 12 h of NaCl stress, application of ACC increased the expression of AtSOS1 and AtAHA1, which encode the plasma membrane (PM) Na⁺/H⁺ antiporters (SOS1) and H⁺-ATPase (H⁺ pumps), respectively. In addition, eATP markedly increased the transcription of AtEIN3, AtEIL1, and AtETR1, and ACC treatment of Col-0 roots under NaCl stress conditions caused upregulation of AtRbohF and AtSOS2/3, which directly contribute to the H₂O₂ and Ca²⁺ signaling pathways, respectively. Briefly, ethylene was triggered by eATP, a novel upstream signaling component, which then activated and strengthened the H₂O₂ and Ca²⁺ signaling pathways to maintain K⁺/Na⁺ homeostasis under salinity.

MicroRNAs (miRNAs) are endogenous small RNAs that play a crucial role in plant growth, development, and environmental stress responses. Reaumuria soongorica is a typical halophyte that is widely distributed in saline–alkali desert regions. Under salt stress, R. soongorica can complete germination, a critical biological process in the life cycle of seed plants. To identify miRNAs and predict target mRNAs involved in seed germination during salt stress, nine small-RNA libraries were constructed and analyzed from R. soongorica seeds treated with various concentrations of NaCl. We also obtained transcriptome data under the same treatment conditions. Further analysis identified 88 conserved miRNAs representing 25 defined families and discovered 13 novel miRNAs from nine libraries. A co-expression analysis was performed on the same samples to identify putative miRNA–mRNA interactions that were responsive to salt stress. A comparative analysis of expression during germination under 273 (threshold) and 43 mM (optimal) NaCl treatments identified 13 differentially expressed miRNAs and 23 corresponding target mRNAs, while a comparison between 43 mM NaCl and non-salt-stress conditions uncovered one differentially expressed miRNA and one corresponding target mRNA. These results provide basic data for further study of molecular mechanisms involved in the germination of salt-stressed R. soongorica seeds, and also provide a reference for the improvement of salt tolerance during plant germination.