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Crop diversity through residue incorporation is the most important method for sustaining soil health. A field study was conducted over five consecutive years (2012–2017) to see the impact of residue incorporartions in Inceptisol of eastern India. The main plot treatments had five cropping systems (CS), namely, fallow−rice−rice (FRR), jute−rice−wheat (JRW), jute−rice−baby corn (JRBc), jute−rice−vegetable pea (JRGp), jute−rice−mustard−mungbean/green gram (JRMMu), which cinsisted of four sub-plots with varied nutrient and crop residue management (NCRM) levels, namely crops with no residue +75% of the recommended dose of fertilizers (RDF) (F1R0), crops with the residue of the previous crops +75% RDF (F1R1), crops with no resiude +100% RDF (F2R0), and crops with residue +100% RDF (F2R1). The highest system productivity was obtained for JRBc (15.3 Mg·ha−1), followed by JRGp (8.81 Mg·ha−1) and JRMMu (7.61 Mg·ha−1); however, the highest sustainability index was found with the JRGp cropping system (0.88), followed by JRMMu (0.82). Among the NCRMs, the highest productivity (8.78 Mg·ha−1) and sustainability index (0.83) were recorded in F2R1. Five soil parameters, namely, bulk density, available K, urease activity, dehydrogenase activity, and soil microbial biomass carbon (SMBC), were used in the minimum data-set (MDS) for the calculation of the soil quality index (SQI). The best attainment of SQI was found in the JRGp system (0.63), closely followed by the JRMMu (0.61) cropping system.
Mukesh Kumar; Sabyasachi Mitra; Sonali Paul Mazumdar; Bijan Majumdar; Amit Ranjan Saha; Shiv Ram Singh; Biswajit Pramanick; Ahmed Gaber; Walaa F. Alsanie; Akbar Hossain. Improvement of Soil Health and System Productivity through Crop Diversification and Residue Incorporation under Jute-Based Different Cropping Systems. Agronomy 2021, 11, 1622 .
AMA StyleMukesh Kumar, Sabyasachi Mitra, Sonali Paul Mazumdar, Bijan Majumdar, Amit Ranjan Saha, Shiv Ram Singh, Biswajit Pramanick, Ahmed Gaber, Walaa F. Alsanie, Akbar Hossain. Improvement of Soil Health and System Productivity through Crop Diversification and Residue Incorporation under Jute-Based Different Cropping Systems. Agronomy. 2021; 11 (8):1622.
Chicago/Turabian StyleMukesh Kumar; Sabyasachi Mitra; Sonali Paul Mazumdar; Bijan Majumdar; Amit Ranjan Saha; Shiv Ram Singh; Biswajit Pramanick; Ahmed Gaber; Walaa F. Alsanie; Akbar Hossain. 2021. "Improvement of Soil Health and System Productivity through Crop Diversification and Residue Incorporation under Jute-Based Different Cropping Systems." Agronomy 11, no. 8: 1622.
The present study aims to improve biomass and biochemical constituents, especially lipid production of Anabaena variabilis and Nostoc muscorum by formulating an optimal growth condition using various concentrations of nutrients (NO3−, PO43− and CO32−) for biodiesel production. The supplementation of the three nutrients by +50% showed the maximum dry weight and biomass productivity, while the macromolecule contents were varied. The depletion of N-NO3− by 50% N-NO3− showed the maximum lipid yield (146.67 mg L−1) in A. variabilis and the maximum carbohydrate contents (285.33 mg L−1) in N. muscorum with an increase of 35% and 30% over control of the synthetic medium, respectively. However, variation in P-PO43− and C-CO32− showed insignificant improving results for all biochemical compositions in both cyanobacteria. A. variabilis was the superior species for lipid and protein accumulation; however, N. muscorum showed the maximum carbohydrate content. Accordingly, A. variabilis was selected for biodiesel production. In A. variabilis, −50% N-NO3− resulted in 35% higher lipid productivity compared to the control. Furthermore, the fatty acid profile and biodiesel quality-related parameters have improved under this condition. This study has revealed the strategies to improve A. variabilis lipid productivity for biodiesel production for small-scale in vitro application in terms of fuel quality under low nitrate levels.
Shimaa M. El Shafay; Ahmed Gaber; Walaa F. Alsanie; Mostafa E. Elshobary. Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production. Sustainability 2021, 13, 9081 .
AMA StyleShimaa M. El Shafay, Ahmed Gaber, Walaa F. Alsanie, Mostafa E. Elshobary. Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production. Sustainability. 2021; 13 (16):9081.
Chicago/Turabian StyleShimaa M. El Shafay; Ahmed Gaber; Walaa F. Alsanie; Mostafa E. Elshobary. 2021. "Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production." Sustainability 13, no. 16: 9081.
Very little is known about the changes that occur in soil organic carbon (SOC) and total nitrogen (TN) under an intensive rice-based cropping system following the change to minimal tillage and increased crop residue retention in the Gangetic Plains of South Asia. The field experiment was conducted for 3 years at Rajbari, Bangladesh to examine the impact of tillage practices and crop residue retention on carbon (C) and nitrogen (N) cycling. The experiment comprised four tillage practices—conventional tillage (CT), zero tillage (ZT), strip-tillage (ST), and bed planting (BP) in combination with two residue retention levels—increased residue (R50%) and low residue (R20%—the current practice). The TN, SOC, and mineral N (NH4+-N and NO3−-N) were measured in the soil at different crop growth stages. After 3 years, ZT, ST, and BP sequestered 12, 11, and 6% more SOC, and 18, 13, and 10% more TN, respectively than the conventional crop establishment practice at 0–5 cm soil depth. The accumulation of SOC and TN was also higher compared to the initial SOC and TN in soil. Among the tillage practices, the maximum SOC and TN sequestration were recorded with ST and with R50% that might be attributed to reduced mineralization of C and N in soil particularly with increased residue retention, since decay rates of potentially mineralizable C was lower in the ST with both the residue retention practices. Increased residue retention and minimum tillage practices after nine consecutive crops has altered the C and N cycling by slowing the in-season turnover of C and N, reducing the level of nitrate-N available to plants in the growing season and increasing retained soil levels of SOC and TN.
Nazmus Salahin; Khairul Alam; Sharif Ahmed; Mohammad Jahiruddin; Ahmed Gaber; Walaa F. Alsanie; Akbar Hossain; Richard W. Bell. Carbon and Nitrogen Mineralization in Dark Grey Calcareous Floodplain Soil Is Influenced by Tillage Practices and Residue Retention. Plants 2021, 10, 1650 .
AMA StyleNazmus Salahin, Khairul Alam, Sharif Ahmed, Mohammad Jahiruddin, Ahmed Gaber, Walaa F. Alsanie, Akbar Hossain, Richard W. Bell. Carbon and Nitrogen Mineralization in Dark Grey Calcareous Floodplain Soil Is Influenced by Tillage Practices and Residue Retention. Plants. 2021; 10 (8):1650.
Chicago/Turabian StyleNazmus Salahin; Khairul Alam; Sharif Ahmed; Mohammad Jahiruddin; Ahmed Gaber; Walaa F. Alsanie; Akbar Hossain; Richard W. Bell. 2021. "Carbon and Nitrogen Mineralization in Dark Grey Calcareous Floodplain Soil Is Influenced by Tillage Practices and Residue Retention." Plants 10, no. 8: 1650.
Acute myeloid leukemia (AML) is a bone marrow malignancy having multiple molecular pathways driving its progress. In recent years, the main causes of AML considered all over the world are genetic variations in cancerous cells. The RUNX1 and FLT3 genes are necessary for the normal hematopoiesis and differentiation process of hematopoietic stem cells into mature blood cells, therefore they are the most common targets for point mutations resulting in AML. We screened 32 CN-AML patients for FLT3-ITD (by Allele-specific PCR) and RUNX1 mutations (by Sanger sequencing). The FLT3 mRNA expression was assessed in all AML patients and its subgroups. Eight patients (25%) carried RUNX1 mutation (K83E) while three patients (9.37%) were found to have internal tandem duplications in FLT3 gene. The RUNX1 mutation data were correlated with clinical parameters and FLT3 gene expression profile. The RUNX1 mutations were observed to be significantly prevalent in older males. Moreover, RUNX1 and FLT3-mutated patients had lower complete remission rate, event-free survival rate, and lower overall survival rate than patients with wild-type RUNX1 and FLT3 gene. The RUNX1 and FLT3 mutant patients with up-regulated FLT3 gene expression showed even worse prognosis. Bradford Assay showed that protein concentration was down-regulated in RUNX1 and FLT3 mutants in comparison to RUNX1 and FLT3 wild-type groups. This study constitutes the first report from Pakistan reporting significant molecular mutation analysis of RUNX1 and FLT3 genes including FLT3 expression evaluation with follow-up. This provides an insight that aforementioned mutations are markers of poor prognosis but the study with a large AML cohort will be useful to further investigate their role in disease biology of AML.
Atia Rehman; Afia Muhammad Akram; Asma Chaudhary; Nadeem Sheikh; Zawar Hussain; Walaa F. Alsanie; Rahat Abdul Rehman; Naila Hameed; Tayyaba Saleem; Amjad Zafar; Muhammad Absar; Zafar Iqbal; Alaa Alhazmi; Hosam Ali Baeshen; Zuhair M Mohammedsaleh; Samina Qamer; Samy Sayed; Ahmed Gaber. RUNX1 mutation and elevated FLT3 gene expression cooperates to induce inferior prognosis in cytogenetically normal acute myeloid leukemia patients. Saudi Journal of Biological Sciences 2021, 28, 4845 -4851.
AMA StyleAtia Rehman, Afia Muhammad Akram, Asma Chaudhary, Nadeem Sheikh, Zawar Hussain, Walaa F. Alsanie, Rahat Abdul Rehman, Naila Hameed, Tayyaba Saleem, Amjad Zafar, Muhammad Absar, Zafar Iqbal, Alaa Alhazmi, Hosam Ali Baeshen, Zuhair M Mohammedsaleh, Samina Qamer, Samy Sayed, Ahmed Gaber. RUNX1 mutation and elevated FLT3 gene expression cooperates to induce inferior prognosis in cytogenetically normal acute myeloid leukemia patients. Saudi Journal of Biological Sciences. 2021; 28 (9):4845-4851.
Chicago/Turabian StyleAtia Rehman; Afia Muhammad Akram; Asma Chaudhary; Nadeem Sheikh; Zawar Hussain; Walaa F. Alsanie; Rahat Abdul Rehman; Naila Hameed; Tayyaba Saleem; Amjad Zafar; Muhammad Absar; Zafar Iqbal; Alaa Alhazmi; Hosam Ali Baeshen; Zuhair M Mohammedsaleh; Samina Qamer; Samy Sayed; Ahmed Gaber. 2021. "RUNX1 mutation and elevated FLT3 gene expression cooperates to induce inferior prognosis in cytogenetically normal acute myeloid leukemia patients." Saudi Journal of Biological Sciences 28, no. 9: 4845-4851.
The formulator of aquatic diets is part of a continuous search for alternative protein sources instead of depreciated fish meal. The utilization of zooplankton as a feed ingredient is an interesting trend due to their high-quality protein content and abundance of essential nutrients. The current study aims to investigate the effects of partial and total replacement of fish meal (FM) by Dahpnia magna meal (DMM) on growth performance, feed utilization, histological, and economic status of mullet, M. cephalus, larvae. In addition to the control diet, 100% FM, D0, four diets containing DMM at different levels were used: 25% (D25), 50% (D50), 75% (D75), and 100% (D100) replacement of fish meal. A total of 300 larvae (0.097 ± 0.001 g) were equally divided into five groups (three replicate per each group) at a density of 200 larvae m−3. The aquariums were renewed at a rate of 30% daily. During the 60-day experimental period, all larvae were fed their respective diets at a level of 20% of live body weight, five times a day (9.00 a.m., 12.00, 15.00, 18.00, and 21.00 p.m.). The results indicated that compared to D0, fish fed D75 recorded the highest significant value of growth, and feed utilization parameters, while fish fed D100 achieved the lowest feed cost and incidence cost, and the highest profit index and economic efficiency ratio. A strong correlation was reported among weight gain, feed conversion ratio and fish meal replacement with DMM; R2 = 0.94 and 0.91, respectively. The fit regression model representing mullet response to FM replacement with DMM is a poly-nominal regression model with maximum response at 75–100%. The histological investigation of the intestine revealed an improvement of histomorphometric indices and goblet cell number with increasing DMM inclusion levels. These findings confirmed that 75% to 100% partial substitution of FM with DMM is the ideal replacement for mullet, M. cephalus, larvae for improving fish growth performance and feed utilization.
Hamdy Abo-Taleb; Mohamed Ashour; Mohamed Elokaby; Mohamed Mabrouk; Mohamed El-Feky; Othman Abdelzaher; Ahmed Gaber; Walaa Alsanie; Abdallah Mansour. Effect of a New Feed Daphnia magna (Straus, 1820), as a Fish Meal Substitute on Growth, Feed Utilization, Histological Status, and Economic Revenue of Grey Mullet, Mugil cephalus (Linnaeus 1758). Sustainability 2021, 13, 7093 .
AMA StyleHamdy Abo-Taleb, Mohamed Ashour, Mohamed Elokaby, Mohamed Mabrouk, Mohamed El-Feky, Othman Abdelzaher, Ahmed Gaber, Walaa Alsanie, Abdallah Mansour. Effect of a New Feed Daphnia magna (Straus, 1820), as a Fish Meal Substitute on Growth, Feed Utilization, Histological Status, and Economic Revenue of Grey Mullet, Mugil cephalus (Linnaeus 1758). Sustainability. 2021; 13 (13):7093.
Chicago/Turabian StyleHamdy Abo-Taleb; Mohamed Ashour; Mohamed Elokaby; Mohamed Mabrouk; Mohamed El-Feky; Othman Abdelzaher; Ahmed Gaber; Walaa Alsanie; Abdallah Mansour. 2021. "Effect of a New Feed Daphnia magna (Straus, 1820), as a Fish Meal Substitute on Growth, Feed Utilization, Histological Status, and Economic Revenue of Grey Mullet, Mugil cephalus (Linnaeus 1758)." Sustainability 13, no. 13: 7093.
Burullus Lake is Egypt’s second most important coastal lagoon. The present study aimed to shed light on the different types of polluted waters entering the lake from various drains, as well as to evaluate the zooplankton community, determine the physical and chemical characteristics of the waters, and study the eutrophication state based on three years of seasonal monitoring from 2017 to 2019 at 12 stations. The results revealed that Rotifera, Copepoda, Protozoa, and Cladocera dominated the zooplankton population across the three-year study period, with a total of 98 taxa from 59 genera and 10 groups detected in the whole-body lake in 2018 and 2019, compared to 93 species from 52 genera in 2017. Twelve representative surface water samples were collected from the lake to determine physicochemical parameters, i.e., temperature, pH, salinity, dissolved oxygen, biological oxygen demand, chemical oxygen demand, ammonia-N, nitrate–N, nitrate-N, total nitrogen, total phosphorus, dissolved reactive phosphorus, and chlorophyll-a, as well as Fe, Cu, Zn, Cr, Ni, Cd, and Pb ions. Based on the calculations of the water quality index (WQI), the lake was classified as having good water quality. However, the trophic state is ranked as hyper-eutrophic and high trophic conditions.
Ahmed Alprol; Ahmed Heneash; Asgad Soliman; Mohamed Ashour; Walaa Alsanie; Ahmed Gaber; Abdallah Mansour. Assessment of Water Quality, Eutrophication, and Zooplankton Community in Lake Burullus, Egypt. Diversity 2021, 13, 268 .
AMA StyleAhmed Alprol, Ahmed Heneash, Asgad Soliman, Mohamed Ashour, Walaa Alsanie, Ahmed Gaber, Abdallah Mansour. Assessment of Water Quality, Eutrophication, and Zooplankton Community in Lake Burullus, Egypt. Diversity. 2021; 13 (6):268.
Chicago/Turabian StyleAhmed Alprol; Ahmed Heneash; Asgad Soliman; Mohamed Ashour; Walaa Alsanie; Ahmed Gaber; Abdallah Mansour. 2021. "Assessment of Water Quality, Eutrophication, and Zooplankton Community in Lake Burullus, Egypt." Diversity 13, no. 6: 268.
World Health Organization classified Listeria monocytogenes as a major notable foodborne pathogen associated with high mortality and hospitalization. The study reports the prevalence, antibiogram, virulence determination and genetic characterization of L. monocytogenes from different food products. A total of 250 food samples, fifty samples each from raw milk, ice cream, minced meat, fish fillet and sausage were collected from the Menoufiya governorate in Egypt. L. monocytogenes was detected in 17 (6.8%) of the tested food samples including minced meat (14%), fish fillet (8%), sausage (6%) and raw milk (6%). The antimicrobial susceptibility assay of 17 L. monocytogenes isolates against seventeen antibiotics belonging to eight antibiotics classes revealed a high susceptibility to norfloxacin (82.3%), amoxicillin-clavulanic acid (76.4%), cefotaxime (70.5%), erythromycin (64.6%), amoxicillin (64.6%), gentamicin (58.7%) and vancomycin (58.7%). While, high resistance was observed against oxytetracycline (76.4%), trimethoprim-sulfamethoxazole (76.4%), chloramphenicol (70.5%), doxycycline (64.6%), levofloxacin (41.2%) and azithromycin (41.2%). Of note, all L. monocytogenes isolates were multidrug-resistant. The multiplex PCR successfully amplified L. monocytogenes in all tested isolates. Screening of the five virulence-related genes revealed the hlyA and iap as the most prevalent genes followed by actA gene, however, the inlA and prfA genes were not detected in any of the studied isolates. The partial 16S rRNA gene sequencing of three L. monocytogenes isolates showed a high nucleotide similarity (99.1–99.8%) between the study isolates and various global clones, and phylogenetic analysis clustered these L. monocytogenes strains with other Listeria species including L. welshimeri, L. seeligeri and L. innocua. This study demonstrates the impact of L. monocytogenes as a major contaminant of various food products and suggests more attention to the awareness and hygienic measures in the food industry.
Eman Abdeen; Walid Mousa; Ola. Harb; Gehad Fath-Elbab; Mohammed Nooruzzaman; Ahmed Gaber; Walaa Alsanie; Ahmed Abdeen. Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt. Foods 2021, 10, 1381 .
AMA StyleEman Abdeen, Walid Mousa, Ola. Harb, Gehad Fath-Elbab, Mohammed Nooruzzaman, Ahmed Gaber, Walaa Alsanie, Ahmed Abdeen. Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt. Foods. 2021; 10 (6):1381.
Chicago/Turabian StyleEman Abdeen; Walid Mousa; Ola. Harb; Gehad Fath-Elbab; Mohammed Nooruzzaman; Ahmed Gaber; Walaa Alsanie; Ahmed Abdeen. 2021. "Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt." Foods 10, no. 6: 1381.
Diabetes Mellitus (DM) is one of the important public health issues worldwide. The Fat mass obesity (FTO) gene rs-9939609 variant identified single nucleotide polymorphism (SNP) with the T to A missense mutation, and has a strong association with T2DM. FTO gene is present on chromosome “16q12.2” comprising of nine exons. FTO gene rs-9939609 a variant is commonly found in the Pakistani Population. The purpose of the study was to alert the population about the rs-9939609 variant SNP, having a strong association with T2DM. Total of 190 participants were included in the present cross-sectional study. To collect the samples non-probability convenience technique was used. subjects were recruited and divided into three groups, normal healthy subjects, obese and T2DM. The patients were selected from the Medicine department Jamshoro/Hyderabad by filling the pre-designed proforma, as well as verbal and written consent taken from study participants. To analysed the data ANOVA Post hoc (Tukey-test) was applied for comparison among groups (P < 0.05) and “SNP-STAT” online software was used for frequencies. The BMI, neck circumference, waist circumference and lipid profile, fasting blood sugar and HbA1c was found significant (p < 0.001) in both genders as compared to control. Homozygous and heterozygous distribution of allelic and genotyping frequency was found in study participants. 37.9 %T/A, 57.4% T/T, and A/A were 4.7%. The FTO gene rs-9939609 variant amplified and have an increased risk of developing T2DM in the Sindh population. Codominant model odd ratio of T/A showed 2.42 (CI)1.23–3.84, with significant p < 0.032. The present study concluded that the FTO gene SNP rs-9939609 variant was found in the population of Hyderabad, Sindh and having strong association with T2DM and obese individuals. Increase BMI, neck and waist circumference are the biomarkers of obesity and causative factors of T2DM.
Farheen Shaikh; Tazeen Shah; Norah Abdullah Bazekh Madkhali; Ahmed Gaber; Walaa F. Alsanie; Sanum Ali; Shafaq Ansari; Muhammad Rafiq; R.Z. Sayyed; Nadir Ali Rind; Khalid Hussain Rind; Akhtar Hussain Shar; Syed Mohammed Basheeruddin Asdaq. Frequency distribution and association of Fat-mass and obesity (FTO) gene SNP rs-9939609 variant with Diabetes Mellitus Type-II population of Hyderabad, Sindh, Pakistan. Saudi Journal of Biological Sciences 2021, 28, 4183 -4190.
AMA StyleFarheen Shaikh, Tazeen Shah, Norah Abdullah Bazekh Madkhali, Ahmed Gaber, Walaa F. Alsanie, Sanum Ali, Shafaq Ansari, Muhammad Rafiq, R.Z. Sayyed, Nadir Ali Rind, Khalid Hussain Rind, Akhtar Hussain Shar, Syed Mohammed Basheeruddin Asdaq. Frequency distribution and association of Fat-mass and obesity (FTO) gene SNP rs-9939609 variant with Diabetes Mellitus Type-II population of Hyderabad, Sindh, Pakistan. Saudi Journal of Biological Sciences. 2021; 28 (8):4183-4190.
Chicago/Turabian StyleFarheen Shaikh; Tazeen Shah; Norah Abdullah Bazekh Madkhali; Ahmed Gaber; Walaa F. Alsanie; Sanum Ali; Shafaq Ansari; Muhammad Rafiq; R.Z. Sayyed; Nadir Ali Rind; Khalid Hussain Rind; Akhtar Hussain Shar; Syed Mohammed Basheeruddin Asdaq. 2021. "Frequency distribution and association of Fat-mass and obesity (FTO) gene SNP rs-9939609 variant with Diabetes Mellitus Type-II population of Hyderabad, Sindh, Pakistan." Saudi Journal of Biological Sciences 28, no. 8: 4183-4190.
Bioactive molecules derived from seaweed extracts are revolutionary bio-stimulants used to enhance plant growth and increase yield production. This study evaluated the effectiveness of a commercially available seaweed liquid extract, namely, True-Algae-Max (TAM®), as a plant growth stimulant on nutritional, and antioxidant activity of Capsicum annuum. Three concentrations of TAM® (0.25, 0.5, and 1%) of various NPK: TAM® ratios were investigated via foliar spray, over 2017 and 2018 cultivation seasons, under greenhouse conditions. TAM® is rich in phytochemical compounds, such as ascorbic acid (1.66 mg g−1), phenolics (101.67 mg g−1), and flavonoids (2.60 mg g−1) that showed good antioxidant activity (54.52 mg g−1) and DPPH inhibition of 70.33%. Promoting measured parameter results stated the extensive potentiality of TAM® application, in comparison with conventional NPK treatment. Yield and composition of C. annuum were significantly improved in all TAM® treated groups, especially the TAM0.5% concentration, which resulted in maximum yield (4.23 Kg m−2) and significant amounts of profuse biological molecules like chlorophyll, ascorbic, phenolic compounds, flavonoids, and total nutrients. Compared to the NPK control treatments, C. annuum treated with TAM0.5% improved the total antioxidant activity of hot Pepper from 162.16 to 190.95 mg g−1. These findings indicate that the extract of seaweed can be used as an environmentally friendly, multi-functional biostimulant in the agricultural field for more sustainable production, in addition to reducing the use of hazardous synthetic fertilizers.
Mohamed Ashour; Shimaa Hassan; Mostafa Elshobary; Gamal Ammar; Ahmed Gaber; Walaa Alsanie; Abdallah Mansour; Rania El-Shenody. Impact of Commercial Seaweed Liquid Extract (TAM®) Biostimulant and Its Bioactive Molecules on Growth and Antioxidant Activities of Hot Pepper (Capsicum annuum). Plants 2021, 10, 1045 .
AMA StyleMohamed Ashour, Shimaa Hassan, Mostafa Elshobary, Gamal Ammar, Ahmed Gaber, Walaa Alsanie, Abdallah Mansour, Rania El-Shenody. Impact of Commercial Seaweed Liquid Extract (TAM®) Biostimulant and Its Bioactive Molecules on Growth and Antioxidant Activities of Hot Pepper (Capsicum annuum). Plants. 2021; 10 (6):1045.
Chicago/Turabian StyleMohamed Ashour; Shimaa Hassan; Mostafa Elshobary; Gamal Ammar; Ahmed Gaber; Walaa Alsanie; Abdallah Mansour; Rania El-Shenody. 2021. "Impact of Commercial Seaweed Liquid Extract (TAM®) Biostimulant and Its Bioactive Molecules on Growth and Antioxidant Activities of Hot Pepper (Capsicum annuum)." Plants 10, no. 6: 1045.
This research aimed to produce new 1-[(aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic acid derivatives and check their anticancer effect against the breast cancer MCF-7 cell line. The 2-oxo-1,2-dihydroquinoline-3-carboxylic acid (4) compound was obtained by hydrolyzing ethyl 2-oxo-1,2-dihydroquinoline-3-carboxylate (2) with thiourea and anhydrous potassium carbonate ethanol, which was then treated with ethyl 3-substituted 2-cyanoacrylates (6) in the presence of triethylamine in diethyl formamide to give 1-[2-(ethoxy)carbonyl-2-cyano-1-arylvinyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic (7a,d). Cyclization of compound 7 with hydrazine hydrate ethanol inferred the association of 1-[(aryl)(3 amino-5-oxopyrazolidin-4-ylidene)methyl-2-oxo-1,2-dihydroquinol-3-carboxylates (8a,d). Spectroscopic and micro-analytical techniques such as IR, NMR, and elemental analysis were used to validate the structure of the synthesized organic compounds. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested by using the MTT assay on the MCF-7 cell line. When compared to the reference compound Dox, the compounds 7b,c and 8a–c demonstrated strong anticancer activity against the MCF-7 cell line. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested against the MCF-7 cell line, using MTT assay. The compounds 7b,c and 8a–c showed significant anticancer activity compared to the reference compound Dox against the MCF-7 cell line.
Ahmed Gaber; Walaa Alsanie; Majid Alhomrani; Abdulhakeem Alamri; Ibrahim El-Deen; Moamen Refat. Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity. Crystals 2021, 11, 571 .
AMA StyleAhmed Gaber, Walaa Alsanie, Majid Alhomrani, Abdulhakeem Alamri, Ibrahim El-Deen, Moamen Refat. Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity. Crystals. 2021; 11 (5):571.
Chicago/Turabian StyleAhmed Gaber; Walaa Alsanie; Majid Alhomrani; Abdulhakeem Alamri; Ibrahim El-Deen; Moamen Refat. 2021. "Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity." Crystals 11, no. 5: 571.
This study aimed to synthesize quinolinone derivatives and investigate their cytotoxic activity. The compound 1-azacoumarin-3-carboxylic acid (2-oxo-1H-quinoline-3-carboxylic acid) was obtained via the cyclocondensation of 2-hydroxybenzaldehyde with diethyl malonate in base catalyst to give ethyl coumarin-3-carboxylate, followed by the ammonolysis of ester (ethyl coumarin-3-carboxylate) with ammonia in the presence of anhydrous potassium carbonate. Treatment of 2-oxo-1H-quinoline-3-carboxylic acid with acetic anhydride, cinnamaldehyde, cinnamic acid and methyl 5-phenyl-2-cyano-2,4-pentadienoate under different conditions led to the formation of 1 (substituted) aza coumarin-3-carboxylic acids (1-N-(acetyl)-azacoumarin-3-carboxylic acid, 1-N-(2-Formyl-1-phenyl) vinyl-azacoumarin-3-carboxylic acids, 1-N-[2-(Hydroxy) carbonyl-1-(Phenyl) vinyl]-azacoumarin-3-carboxylic acid and 1-N-(4-Cyano-5-methoxy-5-oxo-1-Phenylpenta-1,3-diene-1-y)-azacoumarin-3-carboxylic 284 acid), respectively. The structures of synthesized 1-(substituted) azacoumarin-3-carboxylic acids were confirmed based on spectroscopic methods (IR and NMR), along with elemental analyses. Interestingly compound 6 demonstrated probable impacts as an anti-cancer drug against the MCF-7 cell line. The mechanism of action was assessed using a flow cytometric assay. The outcomes revealed that compound 6 could arrest the cell cycle at G2/M phase and pre-G1 apoptosis.
Ahmed Gaber; Walaa Alsanie; Majid Alhomrani; Abdulhakeem Alamri; Ibrahim El-Deen; Moamen Refat. Synthesis and Characterization of Some New Coumarin Derivatives as Probable Breast Anticancer MCF-7 Drugs. Crystals 2021, 11, 565 .
AMA StyleAhmed Gaber, Walaa Alsanie, Majid Alhomrani, Abdulhakeem Alamri, Ibrahim El-Deen, Moamen Refat. Synthesis and Characterization of Some New Coumarin Derivatives as Probable Breast Anticancer MCF-7 Drugs. Crystals. 2021; 11 (5):565.
Chicago/Turabian StyleAhmed Gaber; Walaa Alsanie; Majid Alhomrani; Abdulhakeem Alamri; Ibrahim El-Deen; Moamen Refat. 2021. "Synthesis and Characterization of Some New Coumarin Derivatives as Probable Breast Anticancer MCF-7 Drugs." Crystals 11, no. 5: 565.
Copepods are one of the most abundant and diverse live food sources for mesopelagic and bathypelagic fishes and crustaceans. They could contribute to the overlap of the transition period from live feed to an artificial weaning diet in marine larvae production. However, the culture conditions still need optimization to provide sufficient production to cover the increasing demand for marine hatcheries. Therefore, the present study investigated the effects of different salinity levels (5, 10, 15, 20, 25, and 30 ppt) on the population growth, growth rate, and population composition (males, females, copepodite, and nauplii ratio) of the marine copepod, Oithona nana. The experiment continued for 15 days, under laboratory-controlled conditions of temperature (27 ± 1 °C), pH (7.7 ± 0.15), and continuous gentle aeration in 30 L glass aquaria. The copepod culture aquaria were supplemented with a mixture of soybean and yeast (0.5 g 10−6 individual−1 24-h−1) as a feed source. The highest significant population growth and population growth rate of O. nana were achieved with a salinity level of 20 ppt. Regarding population composition, O. nana cultured at the salinity level of 20 ppt recorded the highest significant percentages of copepodite and nauplii. The results concluded that copepod, O. nana, is capable of withstanding abrupt changes in the salinity, but there are limits to their tolerance, with an optimal salinity level of 20 ppt. This salinity level achieved the highest population growth and the highest percentages of copepodite and nauplii of marine Copepoda, O. nana.
Fawzy Magouz; Mohamed Essa; Mustafa Matter; Abdallah Mansour; Ahmed Gaber; Mohamed Ashour. Effect of Different Salinity Levels on Population Dynamics and Growth of the Cyclopoid Copepod Oithona nana. Diversity 2021, 13, 190 .
AMA StyleFawzy Magouz, Mohamed Essa, Mustafa Matter, Abdallah Mansour, Ahmed Gaber, Mohamed Ashour. Effect of Different Salinity Levels on Population Dynamics and Growth of the Cyclopoid Copepod Oithona nana. Diversity. 2021; 13 (5):190.
Chicago/Turabian StyleFawzy Magouz; Mohamed Essa; Mustafa Matter; Abdallah Mansour; Ahmed Gaber; Mohamed Ashour. 2021. "Effect of Different Salinity Levels on Population Dynamics and Growth of the Cyclopoid Copepod Oithona nana." Diversity 13, no. 5: 190.
The modern broiler industry faces huge challenges to keep high production quality and quantity, especially under environmental heat stress conditions. The negative effect of heat stress on broiler productivity is mediated by oxidative stress induction. The blue-green alga (Spirulina platensis) has many applications in poultry nutrition with the high levels of bioactive antioxidant compounds, which can alleviate the oxidative stress damage induced by high ambient temperature. The current study was designed to investigate the effects of dietary Spirulina inclusion at different levels on growth performance, redox status, carcass traits, meat quality, blood hematology, and metabolites profile of broilers subjected to cyclic heat stress. A total of 300 one-day-old Cobb-500 broiler chicks were recruited. Starting from day 21 to 42 of age, birds were randomly divided into five treatment groups with 6 replicates × 10 birds per group, where the first one was provided with the basal diet and reared under normal thermal conditions (23 ± 1 °C) to serve as a negative control. Meanwhile, the other four groups were exposed to cyclic heat stress (34 ± 1 °C for 8 h per day) and were fed a basal diet supplemented with Spirulina at a concentration of 0, 0.5, 1 or 1.5%. Spirulina supplementation to heat-stressed broilers was able to alleviate the negative impacts of heat stress on the final average daily gain, body weight and feed conversion ratio, with the best impact observed among the chickens fed 1% Spirulina. Hematological results indicate increasing hemoglobin and hematocrit levels with Spirulina supplementation compared to the non-supplemented stressed group. Further, Spirulina supplementation significantly influenced blood lipid metabolites marked by reduced serum cholesterol and low-density lipoprotein (LDL), and increased high-density lipoprotein (HDL) levels. The lipid peroxidation level was reduced (p < 0.05), while the antioxidant enzyme activity was increased with Spirulina supplementation to the heat-stressed group. Spirulina supplementation at 0.5 or 1% improved carcass dressing, breast and leg percentages. It can be concluded that dietary Spirulina supplementation at 0.5 or 1% to broiler reared under heat stress conditions can effectively improve broiler production performance and balance the redox status.
Eman Moustafa; Walaa Alsanie; Ahmed Gaber; Nancy Kamel; Abdulaziz Alaqil; Ahmed Abbas. Blue-Green Algae (Spirulina platensis) Alleviates the Negative Impact of Heat Stress on Broiler Production Performance and Redox Status. Animals 2021, 11, 1243 .
AMA StyleEman Moustafa, Walaa Alsanie, Ahmed Gaber, Nancy Kamel, Abdulaziz Alaqil, Ahmed Abbas. Blue-Green Algae (Spirulina platensis) Alleviates the Negative Impact of Heat Stress on Broiler Production Performance and Redox Status. Animals. 2021; 11 (5):1243.
Chicago/Turabian StyleEman Moustafa; Walaa Alsanie; Ahmed Gaber; Nancy Kamel; Abdulaziz Alaqil; Ahmed Abbas. 2021. "Blue-Green Algae (Spirulina platensis) Alleviates the Negative Impact of Heat Stress on Broiler Production Performance and Redox Status." Animals 11, no. 5: 1243.
Applications of medicinal uses of metals and their complexes have been gaining major clinical significance, especially during the COVID-19 pandemic. The ligation behavior of quercetin (Q), a flavonoid, and Zn metal, i.e., the Zn/Q complex, was fully characterized based on molar conductance, infrared (IR) spectra, elemental analysis, electronic spectra, thermogravimetric analysis, proton nuclear magnetic resonance (1H-NMR), and transmission electron microscopy (TEM) in our lab. Hepatotoxicity was induced by cadmium (CdCl2). A total of 40 male albino rats were randomly distributed into the following four groups: Control, hepatotoxic group (CdCl2), Zn/Q-treated group, and group treated with a combination of CdCl2 and Zn/Q. Serum hepatic enzymes (AST, ALT, and LDH), total protein, and enzymatic and nonenzymatic antioxidant levels were determined. Histology and TEM for hepatic tissues, in addition to the gene expression of SOD as an antioxidant enzyme in the hepatic tissues, were evaluated. The Q/Zn treatment demonstrated potent protective effects against CdCl2-induced sever oxidative stress and suppressed hepatic toxicity, genotoxicity, liver enzyme disturbances, and structural alterations. In conclusion, the Zn/Q complex produced a high potent antioxidant effect against the oxidative injury and genotoxicity induced by CdCl2 and could be considered to be a potent ameliorative hepatoprotective agent against CdCl2 hepatotoxicity, which could be beneficial during the COVID-19 pandemic.
Moamen Refat; Reham Hamza; Abdel A. Adam; Hosam Saad; Adil Gobouri; Ehab Azab; Fawziah Al-Salmi; Tariq Altalhi; Ebtihal Khojah; Ahmed Gaber; Samy El-Megharbel. Antioxidant, Antigenotoxic, and Hepatic Ameliorative Effects of Quercetin/Zinc Complex on Cadmium-Induced Hepatotoxicity and Alterations in Hepatic Tissue Structure. Coatings 2021, 11, 501 .
AMA StyleMoamen Refat, Reham Hamza, Abdel A. Adam, Hosam Saad, Adil Gobouri, Ehab Azab, Fawziah Al-Salmi, Tariq Altalhi, Ebtihal Khojah, Ahmed Gaber, Samy El-Megharbel. Antioxidant, Antigenotoxic, and Hepatic Ameliorative Effects of Quercetin/Zinc Complex on Cadmium-Induced Hepatotoxicity and Alterations in Hepatic Tissue Structure. Coatings. 2021; 11 (5):501.
Chicago/Turabian StyleMoamen Refat; Reham Hamza; Abdel A. Adam; Hosam Saad; Adil Gobouri; Ehab Azab; Fawziah Al-Salmi; Tariq Altalhi; Ebtihal Khojah; Ahmed Gaber; Samy El-Megharbel. 2021. "Antioxidant, Antigenotoxic, and Hepatic Ameliorative Effects of Quercetin/Zinc Complex on Cadmium-Induced Hepatotoxicity and Alterations in Hepatic Tissue Structure." Coatings 11, no. 5: 501.
This study aimed to understand the effect of commercial seaweed extract as a biofertilizer, named True-Algae-Max (TAM®), on the yield, nutritional, antioxidant, and cytotoxic activity of Eruca vesicaria. Three concentrations of TAM® (5, 10, and 15%) were studied by foliar spray over the two cultivation years (2016 and 2017) without any chemical fertilizer, along with a control consisting of synthetic nitrogen, phosphorus and potassium (NPK) fertilizers. The yield and composition of E. vesicaria were significantly improved in all treatments, particularly at 10% concentration of TAM®, which resulted in maximum yield (1.99 kg m−2) and significant amounts of chlorophyll, carotenoids, phenolic compounds, flavonoids and total nutrients. Compared to the NPK control, E. vesicaria grown with 10% of TAM® improved total antioxidant activity from 41.80 to 49.36 mg g−1 and cytotoxicity from 25.30 to 60.40% with an IC50 value 85.7 µg mL−1 against the hepatocellular carcinoma cell line (HepG2). These findings indicate that seaweed extract can generally be used as a safe potential multifunctional biofertilizer in the agricultural field. The use of seaweed as a biofertilizer could potentially help mitigate the adverse effects of main nutrient deficiencies, diminishing the use of chemical fertilizers.
Shimaa Hassan; Mohamed Ashour; Ahmed Soliman; Hesham Hassanien; Walaa Alsanie; Ahmed Gaber; Mostafa Elshobary. The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav. Sustainability 2021, 13, 4485 .
AMA StyleShimaa Hassan, Mohamed Ashour, Ahmed Soliman, Hesham Hassanien, Walaa Alsanie, Ahmed Gaber, Mostafa Elshobary. The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav. Sustainability. 2021; 13 (8):4485.
Chicago/Turabian StyleShimaa Hassan; Mohamed Ashour; Ahmed Soliman; Hesham Hassanien; Walaa Alsanie; Ahmed Gaber; Mostafa Elshobary. 2021. "The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav." Sustainability 13, no. 8: 4485.
The solid adducts of SnCl2.(3amt).H2O, SnCl2.2(3amt).H2O, CdCl2.(3amt), CdCl2.2(3amt), SnCl2.(2mct).0.5H2O, SnCl2.2(2mct), CdCl2.(2mct), CdCl2.2(2mct).H2O, SnCl2.(2mcp).1.5H2O, >2.2(2mcp).4H2O, CdCl2.(2mcp), CdCl2.2(2mcp), SnCl2.(4amt).4H2O, SnCl2.2(4amt).1.5H2O, CdCl2.(4amt).H2O, and CdCl2.2(4amt) (where the 3amt, 4amt, 2mct, and 2mcp represent 3-amino-1,2,4-triazole, 4-amino-1,2,4-triazole, 2-mercaptothiazoline, and 2-mercaptopyridine simple organic chelates, respectively) were prepared using a solid-state route and investigated by CHN elemental analysis and infrared spectroscopy. Additionally, we investigated the thermogravimetric characterization and antimicrobial proprieties. It is verified that for 3amt and 4amt adducts, the coordination occurs through nitrogen atom. For 2mct compounds, the coordination occurs through nitrogen (Sn) or sulfur (Cd). For 2mcp adducts, both coordination sites nitrogen and sulfur are involved. By examination of TG curves, it is confirmed that for each hydrated compounds, the first mass loss step is linked with the release of water molecules followed by the release of ligand molecules and sublimation of the metal chloride. Furthermore, it is verified that, considering only the release of ligand molecules (3amp, 4amp, 2mct, or 2mcp), the cadmium adducts are always more stable than the correspondent tin adducts probably due to the formation of cross-linking bonds in these compounds. Finally, of these 16 adducts, 14 showed antimicrobial activities against different bacterial and fungal strains.
Ahmed Gaber; Walaa F. Alsanie; Robson F. de Farias; Moamen S. Refat. Preparation and Thermogravimetric and Antimicrobial Investigation of Cd (II) and Sn (II) Adducts of Mercaptopyridine, Amino Triazole Derivatives, and Mercaptothiazoline Organic Ligand Moieties. Bioinorganic Chemistry and Applications 2021, 2021, 1 -10.
AMA StyleAhmed Gaber, Walaa F. Alsanie, Robson F. de Farias, Moamen S. Refat. Preparation and Thermogravimetric and Antimicrobial Investigation of Cd (II) and Sn (II) Adducts of Mercaptopyridine, Amino Triazole Derivatives, and Mercaptothiazoline Organic Ligand Moieties. Bioinorganic Chemistry and Applications. 2021; 2021 ():1-10.
Chicago/Turabian StyleAhmed Gaber; Walaa F. Alsanie; Robson F. de Farias; Moamen S. Refat. 2021. "Preparation and Thermogravimetric and Antimicrobial Investigation of Cd (II) and Sn (II) Adducts of Mercaptopyridine, Amino Triazole Derivatives, and Mercaptothiazoline Organic Ligand Moieties." Bioinorganic Chemistry and Applications 2021, no. : 1-10.
Herein, we report the synthesis of eight new mononuclear and binuclear Co2+, Ni2+, Cu2+, and Zn2+ methoxy thiosemicarbazone (MTSC) complexes aiming at obtaining thiosemicarbazone complex with potent biological activity. The structure of the MTSC ligand and its metal complexes was fully characterized by elemental analysis, spectroscopic techniques (NMR, FTIR, UV-Vis), molar conductivity, thermogravimetric analysis (TG), and thermal differential analysis (DrTGA). The spectral and analytical data revealed that the obtained thiosemicarbazone-metal complexes have octahedral geometry around the metal center, except for the Zn2+-thiosemicarbazone complexes, which showed a tetrahedral geometry. The antibacterial and antifungal activities of the MTSC ligand and its (Co2+, Ni2+, Cu2+, and Zn2+) metal complexes were also investigated. Interestingly, the antibacterial activity of MTSC- metal complexes against examined bacteria was higher than that of the MTSC alone, which indicates that metal complexation improved the antibacterial activity of the parent ligand. Among different metal complexes, the MTSC- mono- and binuclear Cu2+ complexes showed significant antibacterial activity against Bacillus subtilis and Proteus vulgaris, better than that of the standard gentamycin drug. The in silico molecular docking study has revealed that the MTSC ligand could be a potential inhibitor for the oxidoreductase protein.
Ahmed Gaber; Moamen Refat; Arafa Belal; Ibrahim El-Deen; Nader Hassan; Rozan Zakaria; Majid Alhomrani; Abdulhakeem Alamri; Walaa Alsanie; Essa M. Saied. New Mononuclear and Binuclear Cu(II), Co(II), Ni(II), and Zn(II) Thiosemicarbazone Complexes with Potential Biological Activity: Antimicrobial and Molecular Docking Study. Molecules 2021, 26, 2288 .
AMA StyleAhmed Gaber, Moamen Refat, Arafa Belal, Ibrahim El-Deen, Nader Hassan, Rozan Zakaria, Majid Alhomrani, Abdulhakeem Alamri, Walaa Alsanie, Essa M. Saied. New Mononuclear and Binuclear Cu(II), Co(II), Ni(II), and Zn(II) Thiosemicarbazone Complexes with Potential Biological Activity: Antimicrobial and Molecular Docking Study. Molecules. 2021; 26 (8):2288.
Chicago/Turabian StyleAhmed Gaber; Moamen Refat; Arafa Belal; Ibrahim El-Deen; Nader Hassan; Rozan Zakaria; Majid Alhomrani; Abdulhakeem Alamri; Walaa Alsanie; Essa M. Saied. 2021. "New Mononuclear and Binuclear Cu(II), Co(II), Ni(II), and Zn(II) Thiosemicarbazone Complexes with Potential Biological Activity: Antimicrobial and Molecular Docking Study." Molecules 26, no. 8: 2288.
Seaweed extract biostimulants are among the best modern sustainable biological plant growth promoters. They have been proven to eliminate plant diseases and abiotic stresses, leading to maximizing yields. Additionally, they have been listed as environmentally friendly biofertilizers. The focus of the present research is the use of a commercial seaweed biostimulant as an eco-friendly product (formally named True Algae Max (TAM). During the 2017 and 2018 seasons, five treatments of various NPK:TAM ratios were applied via regular fertigation, namely a conventional treatment of 100% NPK (C0) alongside combinations of 25%, 50%, 75%, and 100% (C25, C50, C75, and C100) of TAM, to evaluate the effectiveness of its bioactive compounds on enhancing growth, yield, and NPK content of cucumber (Cucumis sativus) under greenhouse conditions. TAM is rich in phytochemical compounds, such as milbemycin oxime, rhodopin, nonadecane, and 5-silaspiro [4.4]nona-1,3,6,8-tetraene,3,8-bis(diethylboryl)-2,7-diethyl-1,4,6,9-tetraphenyl-. Promising measured parameter outcomes showed the potentiality of applying TAM with and without mixes of ordinary NPK application. TAM could increase cucumber yield due to improving chemical and physical features related to immunity, productivity, and stress defense. In conclusion, it is better to avoid applying mineral fertilizers, considering also that the organic agricultural and welfare sectors could shortly depend on such biotechnological tools and use them to fulfill global food demands for improved sustainability.
Shimaa Hassan; Mohamed Ashour; Nobumitsu Sakai; Lixin Zhang; Hesham Hassanien; Ahmed Gaber; Gamal Ammar. Impact of Seaweed Liquid Extract Biostimulant on Growth, Yield, and Chemical Composition of Cucumber (Cucumis sativus). Agriculture 2021, 11, 320 .
AMA StyleShimaa Hassan, Mohamed Ashour, Nobumitsu Sakai, Lixin Zhang, Hesham Hassanien, Ahmed Gaber, Gamal Ammar. Impact of Seaweed Liquid Extract Biostimulant on Growth, Yield, and Chemical Composition of Cucumber (Cucumis sativus). Agriculture. 2021; 11 (4):320.
Chicago/Turabian StyleShimaa Hassan; Mohamed Ashour; Nobumitsu Sakai; Lixin Zhang; Hesham Hassanien; Ahmed Gaber; Gamal Ammar. 2021. "Impact of Seaweed Liquid Extract Biostimulant on Growth, Yield, and Chemical Composition of Cucumber (Cucumis sativus)." Agriculture 11, no. 4: 320.
Substance-use disorder is globally prevalent and responsible for numerous social and medical problems. Pregabalin (Lyrica), typically used to treat diabetic neuropathy, has recently emerged as a drug of abuse. Drug abuse is associated with several neuronal changes, including the downregulation of glutamate transporters such as glutamate transporter 1 and cystine/glutamate antiporter. We investigated the effects of N-acetylcysteine, a glutamate transporter 1 and xCT upregulator, on pregabalin addiction using a conditioned place preference paradigm. Pregabalin (60 mg/kg) was found to induce conditioned place preference when compared to a vehicle. A 100 mg/kg dose of N-acetylcysteine was found to block pregabalin-seeking behaviors. These results support previous findings showing that glutamate transporters play an important role in pregabalin-induced seeking behaviors. N-acetylcysteine may represent a beneficial agent in preventing the abuse potential of pregabalin.
Atiah Almalki; Hashem Alsaab; Walaa Alsanie; Ahmed Gaber; Turki Alkhalifa; Ahmad Almalki; Omar Alzahrani; Ana Hardy; Qasim Alhadidi; Zahoor Shah; Yusuf Althobaiti. Potential Benefits of N-Acetylcysteine in Preventing Pregabalin-Induced Seeking-Like Behavior. Healthcare 2021, 9, 376 .
AMA StyleAtiah Almalki, Hashem Alsaab, Walaa Alsanie, Ahmed Gaber, Turki Alkhalifa, Ahmad Almalki, Omar Alzahrani, Ana Hardy, Qasim Alhadidi, Zahoor Shah, Yusuf Althobaiti. Potential Benefits of N-Acetylcysteine in Preventing Pregabalin-Induced Seeking-Like Behavior. Healthcare. 2021; 9 (4):376.
Chicago/Turabian StyleAtiah Almalki; Hashem Alsaab; Walaa Alsanie; Ahmed Gaber; Turki Alkhalifa; Ahmad Almalki; Omar Alzahrani; Ana Hardy; Qasim Alhadidi; Zahoor Shah; Yusuf Althobaiti. 2021. "Potential Benefits of N-Acetylcysteine in Preventing Pregabalin-Induced Seeking-Like Behavior." Healthcare 9, no. 4: 376.
The extensive usage of gibberellic acid (GA3) in agriculture and plant growth is generally associated with enormous human and public health hazards. The present research assesses the impact of n‐acetyl cysteine (NAC) on the hepatorenal injury persuaded by GA3 for this purpose, After two weeks of adaptation twenty‐four rats allocated into four groups (6 rats/group) as follows: control group, supplied with saline only; n‐acetyl cysteine (NAC) group, provided with 150 mg/kg/bw by stomach tube (orally) dissolved in saline; Positive GA3 group, received GA3 (55 mg/kg/bw) orally; Protective group received NAC (150 mg/kg/bw) and GA3 (55 mg/kg/bw) as in NAC and GA3 groups. Rats received their treatments for consecutive 3 weeks. On day 22, rats were anesthetized, then euthanized. Blood and tissue samples were obtained for biochemical, antioxidants markers analysis, gene expression, and histopathological examination. Our results revealed significant changes in serum AST, ALT, urea, uric acid, total protein, and albumin levels with a substantial rise of MDA and NO concentration in GA3 treated rats along with a considerable decrease of the GSH and overexpression of the inflammatory hepatic and renal cytokines (IL‐10, TNF‐α, NOS) and fibrotic gene expression TGF‐β1, and α‐SMA, with boost expression of nuclear factor‐kappa (NFkB). NAC co‐administered with GA3 significantly normalized the kidney and liver function and the antioxidant state, besides normal histological structure of both liver and kidney tissue and downregulated expression of the pro‐inflammatory cytokines as well as, fibrogenic gene expression. Practical applications The current study confirmed that GA3 induced hepto‐renal dysfunction that was ameliorated by NAC administration. Moreover, our findings confirmed the antioxidant capability of n‐acetyl cysteine and afford robust evidence about the ameliorative effect of the n‐acetyl cysteine to attenuate the hepatorenal injury induced by gibberellic acid through modulation of the antioxidant defense system fibrogenic, and pro‐inflammatory cytokines expression.
Mohamed Mohamed Soliman; Adil Aldhahrani; Ahmed Gaber; Walaa F. Alsanie; Mustafa Shukry; Wafaa Abdou Mohamed; Mohamed M. M. Metwally. Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity. Journal of Food Biochemistry 2021, 45, e13706 .
AMA StyleMohamed Mohamed Soliman, Adil Aldhahrani, Ahmed Gaber, Walaa F. Alsanie, Mustafa Shukry, Wafaa Abdou Mohamed, Mohamed M. M. Metwally. Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity. Journal of Food Biochemistry. 2021; 45 (4):e13706.
Chicago/Turabian StyleMohamed Mohamed Soliman; Adil Aldhahrani; Ahmed Gaber; Walaa F. Alsanie; Mustafa Shukry; Wafaa Abdou Mohamed; Mohamed M. M. Metwally. 2021. "Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity." Journal of Food Biochemistry 45, no. 4: e13706.