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Dr. Hesham Hassanien
Animal and Fish production Dept., Fac. of Agric. and Food Sci. King Faisal Univ. Saudi Arabia

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0 Aquaculture
0 fish ecology
0 Fish Cryopreservation
0 Fish breeding
0 gene markers

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Journal article
Published: 17 April 2021 in Sustainability
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This study aimed to understand the effect of commercial seaweed extract as a biofertilizer, named True-Algae-Max (TAM®), on the yield, nutritional, antioxidant, and cytotoxic activity of Eruca vesicaria. Three concentrations of TAM® (5, 10, and 15%) were studied by foliar spray over the two cultivation years (2016 and 2017) without any chemical fertilizer, along with a control consisting of synthetic nitrogen, phosphorus and potassium (NPK) fertilizers. The yield and composition of E. vesicaria were significantly improved in all treatments, particularly at 10% concentration of TAM®, which resulted in maximum yield (1.99 kg m−2) and significant amounts of chlorophyll, carotenoids, phenolic compounds, flavonoids and total nutrients. Compared to the NPK control, E. vesicaria grown with 10% of TAM® improved total antioxidant activity from 41.80 to 49.36 mg g−1 and cytotoxicity from 25.30 to 60.40% with an IC50 value 85.7 µg mL−1 against the hepatocellular carcinoma cell line (HepG2). These findings indicate that seaweed extract can generally be used as a safe potential multifunctional biofertilizer in the agricultural field. The use of seaweed as a biofertilizer could potentially help mitigate the adverse effects of main nutrient deficiencies, diminishing the use of chemical fertilizers.

ACS Style

Shimaa Hassan; Mohamed Ashour; Ahmed Soliman; Hesham Hassanien; Walaa Alsanie; Ahmed Gaber; Mostafa Elshobary. The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav. Sustainability 2021, 13, 4485 .

AMA Style

Shimaa Hassan, Mohamed Ashour, Ahmed Soliman, Hesham Hassanien, Walaa Alsanie, Ahmed Gaber, Mostafa Elshobary. The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav. Sustainability. 2021; 13 (8):4485.

Chicago/Turabian Style

Shimaa Hassan; Mohamed Ashour; Ahmed Soliman; Hesham Hassanien; Walaa Alsanie; Ahmed Gaber; Mostafa Elshobary. 2021. "The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav." Sustainability 13, no. 8: 4485.

Journal article
Published: 06 April 2021 in Agriculture
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Seaweed extract biostimulants are among the best modern sustainable biological plant growth promoters. They have been proven to eliminate plant diseases and abiotic stresses, leading to maximizing yields. Additionally, they have been listed as environmentally friendly biofertilizers. The focus of the present research is the use of a commercial seaweed biostimulant as an eco-friendly product (formally named True Algae Max (TAM). During the 2017 and 2018 seasons, five treatments of various NPK:TAM ratios were applied via regular fertigation, namely a conventional treatment of 100% NPK (C0) alongside combinations of 25%, 50%, 75%, and 100% (C25, C50, C75, and C100) of TAM, to evaluate the effectiveness of its bioactive compounds on enhancing growth, yield, and NPK content of cucumber (Cucumis sativus) under greenhouse conditions. TAM is rich in phytochemical compounds, such as milbemycin oxime, rhodopin, nonadecane, and 5-silaspiro [4.4]nona-1,3,6,8-tetraene,3,8-bis(diethylboryl)-2,7-diethyl-1,4,6,9-tetraphenyl-. Promising measured parameter outcomes showed the potentiality of applying TAM with and without mixes of ordinary NPK application. TAM could increase cucumber yield due to improving chemical and physical features related to immunity, productivity, and stress defense. In conclusion, it is better to avoid applying mineral fertilizers, considering also that the organic agricultural and welfare sectors could shortly depend on such biotechnological tools and use them to fulfill global food demands for improved sustainability.

ACS Style

Shimaa Hassan; Mohamed Ashour; Nobumitsu Sakai; Lixin Zhang; Hesham Hassanien; Ahmed Gaber; Gamal Ammar. Impact of Seaweed Liquid Extract Biostimulant on Growth, Yield, and Chemical Composition of Cucumber (Cucumis sativus). Agriculture 2021, 11, 320 .

AMA Style

Shimaa Hassan, Mohamed Ashour, Nobumitsu Sakai, Lixin Zhang, Hesham Hassanien, Ahmed Gaber, Gamal Ammar. Impact of Seaweed Liquid Extract Biostimulant on Growth, Yield, and Chemical Composition of Cucumber (Cucumis sativus). Agriculture. 2021; 11 (4):320.

Chicago/Turabian Style

Shimaa Hassan; Mohamed Ashour; Nobumitsu Sakai; Lixin Zhang; Hesham Hassanien; Ahmed Gaber; Gamal Ammar. 2021. "Impact of Seaweed Liquid Extract Biostimulant on Growth, Yield, and Chemical Composition of Cucumber (Cucumis sativus)." Agriculture 11, no. 4: 320.

Journal article
Published: 06 February 2021 in Sustainability
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To achieve strong, successful and commercial aqua-biotechnological microalgae applications, screening, isolation, molecular identification, and physiological characterizations are needed. In the current study, a native cyanobacteria strain Arthrospira platensis NIOF17/003 was isolated from the surface water of El-Khadra Lake, a saline-alkaline lake located in Wadi El-Natrun, Egypt. The cyanobacterium was phylogenetically identified by 16S rRNA molecular marker and deposited in the GenBank database (accession number MW396472). The late exponential phase of A. platensis NIOF17/003 was reached at the 8th day of growth using Zarrouk medium, with a recorded dry weight (DW) of 0.845 g L−1. The isolated strain showed 52% of protein, 14% of carbohydrate, biomass productivity of 143.83 mg L−1 day−1, 8.5% of lipid, and lipid productivity of 14.37 mg L−1 day−1. In general, the values of cetane number, iodine value, cold filter plugging point (52.9, 85.5 g I2/100 g oil, and −2.2 °C, respectively) of the isolated fatty acid methyl esters are in accordance with those suggested by international standards. Besides, applying algal-free lipid (FL) as biodiesel byproduct in the production of rotifer (Brachionus plicatilis) revealed that a 0.6 g L−1 FL significantly increased the rotifer population females carrying eggs, confirming that FL can be used efficiently for B. plicatilis production. The current study concluded that the new isolate A. platensis NIOF17/003 is a promising strain for double sustainable use in biodiesel production and aquaculture feed.

ACS Style

Mohamed Zaki; Mohamed Ashour; Ahmed Heneash; Mohamed Mabrouk; Ahmed Alprol; Hanan Khairy; Abdelaziz Nour; Abdallah Mansour; Hesham Hassanien; Ahmed Gaber; Mostafa Elshobary. Potential Applications of Native Cyanobacterium Isolate (Arthrospira platensis NIOF17/003) for Biodiesel Production and Utilization of Its Byproduct in Marine Rotifer (Brachionus plicatilis) Production. Sustainability 2021, 13, 1769 .

AMA Style

Mohamed Zaki, Mohamed Ashour, Ahmed Heneash, Mohamed Mabrouk, Ahmed Alprol, Hanan Khairy, Abdelaziz Nour, Abdallah Mansour, Hesham Hassanien, Ahmed Gaber, Mostafa Elshobary. Potential Applications of Native Cyanobacterium Isolate (Arthrospira platensis NIOF17/003) for Biodiesel Production and Utilization of Its Byproduct in Marine Rotifer (Brachionus plicatilis) Production. Sustainability. 2021; 13 (4):1769.

Chicago/Turabian Style

Mohamed Zaki; Mohamed Ashour; Ahmed Heneash; Mohamed Mabrouk; Ahmed Alprol; Hanan Khairy; Abdelaziz Nour; Abdallah Mansour; Hesham Hassanien; Ahmed Gaber; Mostafa Elshobary. 2021. "Potential Applications of Native Cyanobacterium Isolate (Arthrospira platensis NIOF17/003) for Biodiesel Production and Utilization of Its Byproduct in Marine Rotifer (Brachionus plicatilis) Production." Sustainability 13, no. 4: 1769.

Journal article
Published: 17 December 2020 in Saudi Journal of Biological Sciences
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Understanding of fish genetic characterization plays a vital role in the conservation and utilization of fish genetic resources of grouper species. The present study was carried out to assess the genetic diversity and phylogenetic relationships in five grouper species, Epinephelus spp. from eastern Saudi Arabian coast using two molecular marker systems, inter simple sequence repeat (ISSR) and microsatellite (SSR) markers. In total, 219 individuals grouper specimens (Epinephelus tauvina, E. coioides, E. bleekeri, E. malabaricus, and E. areolatus) were genotyped with 10 ISSR and 11 SSR selected primers. The ISSR produced 94 DNA fragments, of which 44 were polymorphic with an average of 2.13 fragment per primer. While SSR primers generated 107 alleles, all of them were polymorphic with an average 9.72 per primer. ISSR and SSR techniques demonstrated a high level of gene diversity and genetic distances illustrated by UPGMA dendrograms among the grouper species. The results proved that the SSR markers were highly informative and efficient in detecting genetic variability and relationships of the Epinephelus spp.

ACS Style

Hesham Abdallah Hassanien; Yousof Al-Rashada. Assessment of genetic diversity and phylogenetic relationship among grouper species Epinephelus spp. from the Saudi waters of the Arabian Gulf. Saudi Journal of Biological Sciences 2020, 28, 1779 -1786.

AMA Style

Hesham Abdallah Hassanien, Yousof Al-Rashada. Assessment of genetic diversity and phylogenetic relationship among grouper species Epinephelus spp. from the Saudi waters of the Arabian Gulf. Saudi Journal of Biological Sciences. 2020; 28 (3):1779-1786.

Chicago/Turabian Style

Hesham Abdallah Hassanien; Yousof Al-Rashada. 2020. "Assessment of genetic diversity and phylogenetic relationship among grouper species Epinephelus spp. from the Saudi waters of the Arabian Gulf." Saudi Journal of Biological Sciences 28, no. 3: 1779-1786.

Original article
Published: 27 March 2019 in Aquaculture Research
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In this study, three molecular markers including random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) were used to evaluate genetic variation of green tiger prawn Penaeus semisulcatus collected from two geographically isolated environments; located in the Manifa, Arabian Gulf, Saudi Arabia and Ataka, Suez Gulf, Egypt. Genetic parameters included the percentage of polymorphism (P%), effective alleles (Ne), Nei genetic diversity (H) and Shannon index (I), which were calculated based on molecular data. All three marker systems distinguished genetic variation of P. semisulcatus in various levels. The highest polymorphism (91.30%) was obtained with SSR, followed by ISSR (82.26%) and RAPD markers (62.04%), respectively. Our results indicate that SSR appeared to be the best suited molecular assay for assessing the genetic variation between genotypes of P. semisulcatus. The present study indicated that Manifa and Ataka genotypes were closely related. Moreover, the analysis of variability could require more than one DNA‐based molecular marker techniques.

ACS Style

Hesham Hassanien; Yousof Al‐Rashada. Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers. Aquaculture Research 2019, 50, 1626 -1633.

AMA Style

Hesham Hassanien, Yousof Al‐Rashada. Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers. Aquaculture Research. 2019; 50 (6):1626-1633.

Chicago/Turabian Style

Hesham Hassanien; Yousof Al‐Rashada. 2019. "Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers." Aquaculture Research 50, no. 6: 1626-1633.