Atmospheric and room-temperature plasma (ARTP) is an efficient microbial mutagenesis method with broad application prospects. Compared to traditional methods, ARTP technology can more effectively induce DNA damage and generate stable mutant strains. It is characterized by its simplicity, cost-effectiveness, and avoidance of hazardous chemicals, presenting a vast potential for application. The ARTP technology is widely used in bacterial, fungal, and microalgal mutagenesis for increasing productivity and improving characteristics. In conclusion, ARTP technology holds significant promise in the field of microbial breeding. Through ARTP technology, we can create mutant strains with specific genetic traits and improved performance, thereby increasing yield, improving quality, and meeting market demands. The field of microbial breeding will witness further innovation and progress with continuous refinement and optimization of ARTP technology.

As the first defence for cells to counteract the toxicity of active oxygen, superoxide dismutase (SOD) plays an important role in the response of living organisms to stress and cell differentiation. One extracellular Cu-ZnSOD (ecCu-ZnSOD), and two MnSODs, were identified based on the Volvariella volvacea genome sequence. All three genes have complicated alternative splicing modes during transcription; only when the fourth intron is retained can the Vv_Cu-Znsod1 gene be translated into a protein sequence with SOD functional domains. The expression levels of the three sod genes in the pilei are higher than in the stipe. The Vv_Cu-Znsod1 and the Vv_Mnsod2 are co-expressed in different developmental stages of the fruiting body, with the highest level of expression in the pilei of the egg stage, and they show a significant, positive correlation with the efficiency of karyogamy, indicating the potential role of these two genes during karyogamy. The expression of the ecCu-Znsod and two Vv_Mnsod genes showed a significant up-regulated when treated by cold stress for one hour; however, the lack of the intracellular Cu-ZnSOD encoding gene (icCu-Znsod) and the special locus of the ecCu-Znsod gene initiation codon suggested a possible reason for the autolysis phenomenon of V. volvacea in cold conditions.

Evaluation of tourism competitiveness is useful for measuring the level of regional tourism development. It is of great importance to understand the advantages and disadvantages of tourism development correctly and formulate corresponding development strategies. To investigate tourism competitiveness, this paper established an evaluation index system, including tourism development competitiveness, tourism resource competitiveness, and tourism-support competitiveness, for 14 prefectures and cities in Xinjiang in China. The characteristics and laws of spatial differentiation were analyzed. Factor analysis was applied to examine the spatial differentiation of regional tourism competitiveness. The results showed an obvious spatial differentiation in tourism competitiveness among the 14 prefectures and cities. In terms of development competitiveness, Yili and Urumqi constituted the spatial center, followed by Changji, Altay, and Ba Prefecture. As the provincial capital, Urumqi has political, economic, cultural, transportation, and geographic advantages, but its competitiveness is not prominent in terms of monopoly and efficiency. In terms of resource competitiveness, Yili is the core attraction, while Urumqi, Kashgar, Altay, and Ba Prefecture are dominant attractions. With respect to supporting competitiveness, Bo Prefecture has high value, followed by Urumqi City and Aksu. Hetian and Ke Prefecture have the lowest values. The comprehensive competitiveness of tourism is centered on Yili. Urumqi and Bo Prefecture are subcenters, and Changji, Altay, Ba Prefecture, Aksu, and Kashgar are characterized as multi-polar competition areas. Using the KMO and Bartlett’s sphericity tests, the cumulative contribution variance of the eigenvalues of the eight factors extracted by the maximum variance rotation method was found to be 92.714%. Socio-economic conditions, tourism resources, infrastructure construction, regional cultural influence, ecological environment carrying capacity, tertiary industry development, tourism service level, and living security system are the main driving factors affecting the spatial differentiation of tourism competitiveness in Xinjiang. Analyzing the spatial evolution characteristics and the driving factors of the regional tourism competitiveness in Xinjiang, this paper seeks to promote the optimal allocation of tourism production factors in the macro regional system, and provide theoretical guidance and an empirical basis for the comprehensive and harmonic development of regional tourism.

Carbon dioxide is commonly used as one of the significant environmental factors to control pileus expansion during mushroom cultivation. However, the pileus expansion mechanism related to CO₂ is still unknown. In this study, the young fruiting bodies of a popular commercial mushroom Flammulina filiformis were cultivated under different CO₂ concentrations. In comparison to the low CO₂ concentration (0.05%), the pileus expansion rates were significantly lower under a high CO₂ concentration (5%). Transcriptome data showed that the up-regulated genes enriched in high CO₂ concentration treatments mainly associated with metabolism processes indicated that the cell metabolism processes were active under high CO₂ conditions. However, the gene ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with cell division processes contained down-regulated genes at both 12 h and 36 h under a high concentration of CO₂. Transcriptome and qRT-PCR analyses demonstrated that a high CO₂ concentration had an adverse effect on gene expression of the ubiquitin–proteasome system and cell cycle–yeast pathway, which may decrease the cell division ability and exhibit an inhibitory effect on early pileus expansion. Our research reveals the molecular mechanism of inhibition effects on early pileus expansion by elevated CO₂, which could provide a theoretical basis for a CO₂ management strategy in mushroom cultivation.

The mushroom stipe raises the pileus above the substrate into a suitable position for dispersing spores. The stipe elongates at different speeds along its length, with the rate of elongation decreasing in a gradient from the top to the base. However, the molecular mechanisms underlying stipe gradient elongation are largely unknown. Here, we used the model basidiomycete mushroom Flammulina filiformis to investigate the mechanism of mushroom stipe elongation and the role of reactive oxygen species (ROS) signaling in this process. Our results show that O₂⁻ and H₂O₂ exhibit opposite gradient distributions in the stipe, with higher O₂⁻ levels in the elongation region (ER), and higher H₂O₂ levels in the stable region (SR). Moreover, NADPH-oxidase-encoding genes are up-regulated in the ER, have a function in producing O₂⁻, and positively regulate stipe elongation. Genes encoding manganese superoxide dismutase (MnSOD) are up-regulated in the SR, have a function in producing H₂O_2, and negatively regulate stipe elongation. Altogether, our data demonstrate that ROS (O₂⁻/H₂O₂) redistribution mediated by NADPH oxidase and MnSODs is linked to the gradient elongation of the F. filiformis stipe.

Stipe gradient elongation is an important and remarkable feature in the development of most mushroom fruiting bodies. However, its molecular mechanism has rarely been described. Here, the decreasing trend of stipe elongation and increasing trend of cell length in a gradient from the top to the base of the stipe were determined in a model basidiomycete mushroom: Flammulina filiformis. According to RNA-seq results, 1409 differentially expressed genes (DEGs) were identified among elongation region (ER), transition region (TR), and stable region (SR) samples, including 26 transcription factors (TFs). Based on Short Time-series Expression Miner (STEM) clustering of DEGs, clusters 1 and 3, with obvious expression trends that were consistent with or in contrast to the elongation rate, were screened. The cluster 1 DEGs were mainly involved in the GO cellular component category and KEGG genetic information processing class; however, the cluster 3 DEGs were mainly involved in metabolic processes. Furthermore, qRT-PCR confirmed that key genes of the long-chain fatty acid synthesis pathway were involved in stipe gradient elongation and regulated by NADPH oxidase-derived ROS signaling molecules. These findings provide an essential basis for understanding the molecular mechanism of stipe gradient elongation.