Cellular senescence may contribute to aging and age-related diseases and senolytic drugs that selectively kill senescent cells may delay aging and promote healthspan. More recently, several categories of senolytics have been established, namely HSP90 inhibitors, Bcl-2 family inhibitors and natural compounds such as quercetin and fisetin. However, senolytic and senostatic potential of nanoparticles and surface-modified nanoparticles has never been addressed. In the present study, quercetin surface functionalized Fe₃O₄ nanoparticles (MNPQ) were synthesized and their senolytic and senostatic activity was evaluated during oxidative stress-induced senescence in human fibroblasts in vitro. MNPQ promoted AMPK activity that was accompanied by non-apoptotic cell death and decreased number of stress-induced senescent cells (senolytic action) and the suppression of senescence-associated proinflammatory response (decreased levels of secreted IL-8 and IFN-β, senostatic action). In summary, we have shown for the first time that MNPQ may be considered as promising candidates for senolytic- and senostatic-based anti-aging therapies.

Magnetic nanoparticles Fe₃O₄ doped by different amounts of Y³⁺ (0, 0.1, 1, 10 %) ions were designed to obtain maximum heating efficiency in magnetic hyperthermia for cancer treatment. Single phase formation was evident by XRD measurements. Improved magnetization value was obtained for the Fe₃O₄ sample with 1% of Y³⁺ doping. The specific absorption rate (SAR) and intrinsic loss of power (ILP) values for prepared colloids was obtained in water. The best results was estimated for Fe₃O₄ with 0.1% of Y³⁺ ions (SAR=194 W/g and ILP=1.85 nHm²/kg for magnetic field of 16kA/m with the frequency 413 kHz). The excellent biocompatibility with low cell cytotoxicity of Fe₃O₄:Y nanoparticles was observed. Immediately after magnetic hyperthermia treatment with Fe₃O₄:0.1%Y, the 4T1 cells viability decrease was observed (77% for 35 μg/ml, 68 % for 100 μg/ml). These results suggest that Fe₃O₄ doped by Y³⁺ ions nanoparticles are suitable for biomedical applications, especially for the hyperthermia treatment.