We investigated nutrition as a potential mechanism underlying the link between floral diversity/composition and wild bee performance. The health, resilience, and fitness of bees may be limited by a lack of nutritionally balanced larval food (pollen), influencing the entire population, even if adults are not limited nutritionally by the availability and quality of their food (mainly nectar). We hypothesized that the nutritional quality of bee larval food is indirectly connected to the species diversity of pollen provisions and is directly driven by the pollen species composition. Therefore, the accessibility of specific, nutritionally desirable key plant species for larvae might promote bee populations. Using a fully controlled feeding experiment, we simulated different pollen resources that could be available to bees in various environments, reflecting potential changes in floral species diversity and composition that could be caused by landscape changes. Suboptimal concentrations of certain nutrients in pollen produced by specific plant species resulted in reduced bee fitness. The negative effects were alleviated when scarce nutrients were added to these pollen diets. The scarcity of specific nutrients was associated with certain plant species but not with plant diversity. Thus, one of the mechanisms underlying the decreased fitness of wild bees in homogenous landscapes may be nutritional imbalance, i.e., the scarcity of specific nutrients associated with the presence of certain plant species and not with species diversity in pollen provisions eaten by larvae. Accordingly, we provide a conceptual representation of how the floral species composition and diversity can impact bee populations by affecting fitness-related life history traits. Additionally, we suggest that mixes of ‘bee-friendly’ plants used to improve the nutritional base for wild bees should be composed considering the local flora to supplement bees with vital nutrients that are scarce in the considered environment.

Bee nutrition studies have focused on food quantity rather than quality, and on details of bee biology rather than on the functioning of bees in ecosystems. Ecological stoichiometry has been proposed for studies on bee nutritional ecology as an ecosystem-oriented approach complementary to traditional approaches. It uses atomic ratios of chemical elements in foods and organisms as metrics to ask ecological questions. However, information is needed on the fitness effects of nutritional mismatches between bee demand and the supply of specific elements in food. We performed the first laboratory feeding experiment on the wild bee Osmia bicornis, investigating the impact of Na, K, and Zn scarcity in larval food on fitness-related life history traits (mortality, cocoon development, and imago body mass). We showed that bee fitness is shaped by chemical element availability in larval food; this effect may be sex-specific, where Na might influence female body mass, while Zn influences male mortality and body mass, and the trade-off between K allocation in cocoons and adults may influence cocoon and body development. These results elucidate the nutritional mechanisms underlying the nutritional ecology, behavioral ecology, and population functioning of bees within the context of nutrient cycling in the food web.

The energy budget is an indicator of an organism’s overall condition. Changes in energy reserves and/or energy consumption have been used as biomarkers of toxic stress. To understand the effects of different forms and concentrations of Zn and the costs of effective Zn regulation by the earthworm Eisenia andrei, we performed a toxicokinetic experiment in which individuals were sampled over time to determine the available energy reserves (total carbohydrate, protein, and lipid content), energy consumption (measured at the cellular level and as the whole-animal respiration rate), and internal Zn concentration. The earthworms were exposed to ZnCl₂ or zinc nanoparticles (ZnO-NPs) in Lufa 2.2 soil for 21 days (contamination phase), followed by 14 days of elimination in clean soil (decontamination phase). Carbohydrates were the only energy reserves with significantly lower levels following ZnO-NP 1000 treatment than following other treatments (p ≤ 0.00001) in the contamination phase. The total available energy reserves and protein content did not differ among treatments, but a significant effect of exposure time was observed (p ≤ 0.0001). Exposure to Zn (both ions and NPs) increased energy consumption at the cellular level, reflecting the high energy demand of the stress response. The results indicated that E. andrei can regulate internal Zn concentrations efficiently, regardless of form or concentration, without considerable impact on energy reserves or respiration rate.

In this study, the effects of zinc nanoparticles (ZnO-NPs) and ions (ZnCl₂) on the mortality, growth, maturation, and cellular respiration of the earthworm Eisenia andrei were assessed. Earthworms were individually exposed for 98 days, starting from the juvenile stage, to soils contaminated with either ZnO-NPs or ZnCl₂ (125, 250, 500 and 1000 mg Zn kg⁻¹ dry weight (dw)). Exposure to the highest-concentration ionic treatments (500 and 1000 mg kg⁻¹) caused 100% mortality, while for other treatments, mortality did not exceed 15% at the end of exposure. Compared to the control treatment, both 125–1000 mg kg⁻¹ ZnO-NPs and 125 or 250 mg kg⁻¹ ZnCl₂ stimulated earthworm growth, which might be due to a hormetic effect. ZnO-NPs and ZnCl₂ caused different responses at medium Zn concentrations (250 and 500 mg kg⁻¹): earthworms exposed to ionic treatment at 250 mg kg⁻¹ were characterized by a significantly lower growth constant, lower cellular respiration rate, later inflection point, and higher final body weight than those exposed to ZnO-NPs treatments at the same (250 mg kg⁻¹) or twice as high (500 mg kg⁻¹) nominal Zn concentrations. However, differences were not observed in all examined parameters between the studied forms when the highest-concentration ZnO-NPs treatment was compared with the lowest-concentration ionic treatment, which was likely due to the same levels of available Zn concentrations in those treatments. Overall, different growth and maturation strategies accompanied by pronounced differences in cellular respiration were adopted by earthworms exposed to low and medium levels of either ZnO-NPs or ZnCl₂.

Bees provide important ecological services, and many species are threatened globally, yet our knowledge of wild bee ecology and evolution is limited. While evolving from carnivorous ancestors, bees had to develop strategies for coping with limitations imposed on them by a plant‐based diet, with nectar providing energy and essential amino acids and pollen as an extraordinary, protein‐ and lipid‐rich food nutritionally similar to animal tissues. Both nectar and pollen display one characteristic common to plants, a high ratio of potassium to sodium (K:Na), potentially leading to bee underdevelopment, health problems, and death. We discuss why and how the ratio of K:Na contributes to bee ecology and evolution and how considering this factor in future studies will provide new knowledge, more accurately depicting the relationship of bees with their environments. Such knowledge is essential for understanding how plants and bees function and interact and is needed to effectively protect wild bees.