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Dr. Niki Mougiou
Aristotle University of Thessaloniki

Basic Info


Research Keywords & Expertise

0 Metabolic Engineering
0 Molecular Biology
0 Olive
0 Arabidopsis thaliana
0 Genetic

Honors and Awards

BBSRC scholarship

University of East Anglia




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Short Biography

Dr. Niki Mougiou is a biologist and a Research Associate at the Institute of Applied Biosciences - Centre for Research and Technology Hellas. Her scientific interests involve the analysis of olive cultivars, the study of the genes participating in antioxidant biosynthesis in olive fruit and the heterologous production of the antioxidants in yeast bio- factories. Her current research is mostly focused on population genetics and marker assisted breeding of cultivated plants using SSR/ ISSR as well as in genetic analysis of cultivars using Next Generation Sequencing.

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Journal article
Published: 14 April 2021 in Cells
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Histone acetylation is directly related to gene expression. In yeast, the acetyltransferase general control nonderepressible-5 (GCN5) targets histone H3 and associates with transcriptional co-activators alteration/deficiency in activation-2 (ADA2) and alteration/deficiency in activation-3 (ADA3) in complexes like SAGA. Arabidopsis thaliana has two genes encoding proteins, designated ADA3a and ADA3b, that correspond to yeast ADA3. We investigated the role of ADA3a and ADA3b in regulating gene expression during flowering time. Specifically, we found that knock out mutants ada3a-2 and the double mutant ada3a-2 ada3b-2 lead to early flowering compared to the wild type plants under long day (LD) conditions and after moving plants from short days to LD. Consistent with ADA3a being a repressor of floral initiation, FLOWERING LOCUS T (FT) expression was increased in ada3a mutants. In contrast, other genes involved in multiple pathways leading to floral transition, including FT repressors, players in GA signaling, and members of the SPL transcriptional factors, displayed reduced expression. Chromatin immunoprecipitation analysis revealed that ADA3a affects the histone H3K14 acetylation levels in SPL3, SPL5, RGA, GAI, and SMZ loci. In conclusion, ADA3a is involved in floral induction through a GCN5-containing complex that acetylates histone H3 in the chromatin of flowering related genes.

ACS Style

Stylianos Poulios; Despoina Dadarou; Maxim Gavriilidis; Niki Mougiou; Nestoras Kargios; Vasileia Maliori; Amy Hark; John Doonan; Konstantinos Vlachonasios. The Transcriptional Adaptor Protein ADA3a Modulates Flowering of Arabidopsis thaliana. Cells 2021, 10, 904 .

AMA Style

Stylianos Poulios, Despoina Dadarou, Maxim Gavriilidis, Niki Mougiou, Nestoras Kargios, Vasileia Maliori, Amy Hark, John Doonan, Konstantinos Vlachonasios. The Transcriptional Adaptor Protein ADA3a Modulates Flowering of Arabidopsis thaliana. Cells. 2021; 10 (4):904.

Chicago/Turabian Style

Stylianos Poulios; Despoina Dadarou; Maxim Gavriilidis; Niki Mougiou; Nestoras Kargios; Vasileia Maliori; Amy Hark; John Doonan; Konstantinos Vlachonasios. 2021. "The Transcriptional Adaptor Protein ADA3a Modulates Flowering of Arabidopsis thaliana." Cells 10, no. 4: 904.

Journal article
Published: 11 March 2021 in Polish Journal of Food and Nutrition Sciences
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ACS Style

Niki Mougiou; Foteini Trikka; Sofia Michailidou; Mariana Pantoura; Anagnostis Argiriou. Molecular and Biochemical Characterization of the Greek Pepper (Capsicum annuum) Cultivars ‘Florinis’ and ‘Karatzova’. Polish Journal of Food and Nutrition Sciences 2021, 71, 89 -96.

AMA Style

Niki Mougiou, Foteini Trikka, Sofia Michailidou, Mariana Pantoura, Anagnostis Argiriou. Molecular and Biochemical Characterization of the Greek Pepper (Capsicum annuum) Cultivars ‘Florinis’ and ‘Karatzova’. Polish Journal of Food and Nutrition Sciences. 2021; 71 (1):89-96.

Chicago/Turabian Style

Niki Mougiou; Foteini Trikka; Sofia Michailidou; Mariana Pantoura; Anagnostis Argiriou. 2021. "Molecular and Biochemical Characterization of the Greek Pepper (Capsicum annuum) Cultivars ‘Florinis’ and ‘Karatzova’." Polish Journal of Food and Nutrition Sciences 71, no. 1: 89-96.

Review
Published: 05 February 2021 in Plants
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Transcription of protein-encoding genes starts with forming a pre-initiation complex comprised of RNA polymerase II and several general transcription factors. To activate gene expression, transcription factors must overcome repressive chromatin structure, which is accomplished with multiprotein complexes. One such complex, SAGA, modifies the nucleosomal histones through acetylation and other histone modifications. A prototypical histone acetyltransferase (HAT) known as general control non-repressed protein 5 (GCN5), was defined biochemically as the first transcription-linked HAT with specificity for histone H3 lysine 14. In this review, we analyze the components of the putative plant SAGA complex during plant evolution, and current knowledge on the biological role of the key components of the HAT module, GCN5 and ADA2b in plants, will be summarized.

ACS Style

Konstantinos Vlachonasios; Stylianos Poulios; Niki Mougiou. The Histone Acetyltransferase GCN5 and the Associated Coactivators ADA2: From Evolution of the SAGA Complex to the Biological Roles in Plants. Plants 2021, 10, 308 .

AMA Style

Konstantinos Vlachonasios, Stylianos Poulios, Niki Mougiou. The Histone Acetyltransferase GCN5 and the Associated Coactivators ADA2: From Evolution of the SAGA Complex to the Biological Roles in Plants. Plants. 2021; 10 (2):308.

Chicago/Turabian Style

Konstantinos Vlachonasios; Stylianos Poulios; Niki Mougiou. 2021. "The Histone Acetyltransferase GCN5 and the Associated Coactivators ADA2: From Evolution of the SAGA Complex to the Biological Roles in Plants." Plants 10, no. 2: 308.

Journal article
Published: 02 December 2020 in Sustainability
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Olive tree growth and reproduction are severely affected by temperature extremes, compromising fruit yield. In that aspect, the olive varieties “Koroneiki” and “Mastoidis” were employed in a mild cold stress experiment, imitating night frost incidents to assess their biochemical, physiological and reproductive functions in relation to genotype. The physiological performance of the stressed plants was not significantly altered, suggesting that both cultivars were well adapted to mild cold night stress. The biochemical response of the plants, regarding antioxidant enzymes, H2O2 and TBARS accumulation, confirmed that both cultivars could cope with the stress applied. The mRNA levels of the PPO gene, which participates in hydroxytyrosol biosynthesis and plant defense, were elevated after 24-h stress at 0 °C, in both cultivars with “Mastoidis” plants exhibiting higher levels for a longer period. Three more genes involved in hydroxytyrosol biosynthesis upregulated their expression levels as a response to cold stress. The numerous plant phenology aspects measured reinforced the conclusion that both cultivars responded to the stress applied. The results of the present study may contribute to better understanding olive tree adaptive responses to low temperature events, an abiotic stress condition that is often present in an open plantation, thus assisting farmers on breeding and cultivar selection.

ACS Style

Niki Mougiou; Boushra Baalbaki; Georgios Doupis; Nektarios Kavroulakis; Stylianos Poulios; Konstantinos Vlachonasios; Georgios Koubouris. The Effect of Low Temperature on Physiological, Biochemical and Flowering Functions of Olive Tree in Relation to Genotype. Sustainability 2020, 12, 10065 .

AMA Style

Niki Mougiou, Boushra Baalbaki, Georgios Doupis, Nektarios Kavroulakis, Stylianos Poulios, Konstantinos Vlachonasios, Georgios Koubouris. The Effect of Low Temperature on Physiological, Biochemical and Flowering Functions of Olive Tree in Relation to Genotype. Sustainability. 2020; 12 (23):10065.

Chicago/Turabian Style

Niki Mougiou; Boushra Baalbaki; Georgios Doupis; Nektarios Kavroulakis; Stylianos Poulios; Konstantinos Vlachonasios; Georgios Koubouris. 2020. "The Effect of Low Temperature on Physiological, Biochemical and Flowering Functions of Olive Tree in Relation to Genotype." Sustainability 12, no. 23: 10065.

Journal article
Published: 01 July 2018 in Plant Physiology and Biochemistry
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Olive tree is one of the most valuable crops cultivated for its oil that is rich in antioxidants. The beneficial effects of oleuropein and hydroxytyrosol (HT), the most abundant and the most powerful antioxidant respectively, as well as tyrosol, HT's precursor molecule, are well studied however their biosynthetic pathways are not yet clarified. The transcriptome analysis of the young olive fruit, cultivar "Koroneiki", revealed transcripts of all the enzymes used to reconstitute the biosynthetic pathway of tyrosol and HT in other organisms. We also identified transcripts of the genes that encode for enzymes involved in the secologanin biosynthesis, oleuropein's precursor molecule. Following the transcriptome analysis, the relative expression of the transcripts was monitored during fruit development and compared to the concentration of the 3 metabolites they synthesize at the same developmental stages. The highest expression levels, accompanied by the maximum concentration of the three metabolites, was found in the young olive fruit. The correlation between the expression profile and the metabolites' concentration indicates that the transcripts were correctly identified and the synthesis of the compounds is regulated at a transcriptional level. Interestingly, HT showed a sudden increment in the final developmental stage of the black mature fruit that is attributed to oleuropein catabolism.

ACS Style

Niki Mougiou; Fotini Trikka; Emmanouil Trantas; Filippos Ververidis; Antonios Makris; Anagnostis Argiriou; Konstantinos E. Vlachonasios. Expression of hydroxytyrosol and oleuropein biosynthetic genes are correlated with metabolite accumulation during fruit development in olive, Olea europaea , cv. Koroneiki. Plant Physiology and Biochemistry 2018, 128, 41 -49.

AMA Style

Niki Mougiou, Fotini Trikka, Emmanouil Trantas, Filippos Ververidis, Antonios Makris, Anagnostis Argiriou, Konstantinos E. Vlachonasios. Expression of hydroxytyrosol and oleuropein biosynthetic genes are correlated with metabolite accumulation during fruit development in olive, Olea europaea , cv. Koroneiki. Plant Physiology and Biochemistry. 2018; 128 ():41-49.

Chicago/Turabian Style

Niki Mougiou; Fotini Trikka; Emmanouil Trantas; Filippos Ververidis; Antonios Makris; Anagnostis Argiriou; Konstantinos E. Vlachonasios. 2018. "Expression of hydroxytyrosol and oleuropein biosynthetic genes are correlated with metabolite accumulation during fruit development in olive, Olea europaea , cv. Koroneiki." Plant Physiology and Biochemistry 128, no. : 41-49.

Journal article
Published: 26 August 2011 in Molecular Breeding
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The TATA binding protein-associated factor 5 (TAF5) is a subunit of TFIID and SAGA complexes involved in RNA polymerase II transcription initiation and histone acetylation. Although members of the putative SAGA complex in Arabidopsis such as GCN5 and ADA2b have important roles in plant development and abiotic stress responses, the function of other components of the Arabidopsis putative SAGA complex, like TAF5, is unknown. We used reverse genetics to elucidate the biological role of TAF5 in Arabidopsis thaliana. The absence of homozygote taf5 mutants indicated that AtTAF5 is an essential gene for the plant viability. Genetic approaches also revealed that AtTAF5 plays a critical role in regulatory mechanisms involved in male gametogenesis and pollen tube growth. Moreover, Arabidopsis taf5 heterozygous mutants displayed terminal flower-like phenotype, suggesting that TAF5 could be involved in molecular mechanisms that regulate indeterminate inflorescence meristems. Therefore, this work suggests that Arabidopsis TAF5 is necessary and sufficient for a complete plant life cycle.

ACS Style

Niki Mougiou; Stylianos Poulios; Athanasios Kaldis; Konstantinos E. Vlachonasios. Arabidopsis thaliana TBP-associated factor 5 is essential for plant growth and development. Molecular Breeding 2011, 30, 355 -366.

AMA Style

Niki Mougiou, Stylianos Poulios, Athanasios Kaldis, Konstantinos E. Vlachonasios. Arabidopsis thaliana TBP-associated factor 5 is essential for plant growth and development. Molecular Breeding. 2011; 30 (1):355-366.

Chicago/Turabian Style

Niki Mougiou; Stylianos Poulios; Athanasios Kaldis; Konstantinos E. Vlachonasios. 2011. "Arabidopsis thaliana TBP-associated factor 5 is essential for plant growth and development." Molecular Breeding 30, no. 1: 355-366.