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Dr. Georgios Koubouris
ELGO DIMITRA, Institute for Olive Tree, Subtropical Crops and Viticulture

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0 Climate Change
0 Remote Sensing
0 Soil
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Research article
Published: 18 May 2021 in Plant Disease
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In November 2019, a severe outbreak of fruit rot was observed in olive orchards in Crete, southern Greece. Symptoms appeared primarily on fruits and stalks, resembling those caused by anthracnose. Typical symptoms were fruit rot, shrinkage and mummification, associated commonly with stalk discoloration and fruit drop. Disease incidence was estimated up to 100% in some cases and an unprecedented increase in olive oil acidity reaching up to 8% (percentage of oleic acid) in severely affected olive groves was recorded. Thirty-two olive groves were then surveyed, and samples of fruit, stalk, leaf and shoot were collected. Visual, stereoscopic and microscopic observations revealed several fungi belonging to the genera Alternaria, Botryosphaeria, Capnodium, Colletotrichum, Fusarium and Pseudocercospora. Fungal infection in fruits was commonly associated with concomitant infestation by the olive fruit fly Bactrocera oleae along with increased air temperature and relative humidity conditions that prevailed in October-November 2019. Twenty representative fungal strains isolated from symptomatic fruits and stalks were characterized by morphological, physiological and molecular analyses. By rDNA-ITS region and EF1-α gene sequencing analysis, these isolates were identified as Alternaria spp., A. infectoria, Botryosphaeria dothidea, Colletotrichum boninense sensu lato, Fusarium lateritium, F. solani species complex and Stemphylium amaranthi. Pathogenicity tests on punctured fruits revealed that all isolates were pathogenic; however F. solani isolates along with B. dothidea were the most virulent and wounds were necessary for efficient fungal infection. Moreover, as little as 10 spores of F. solani were sufficient of causing significant infection in punctured fruits. Fusarium solani was also capable of infecting olive fruits in the presence of B. oleae, with no additional wounding, in artificial inoculation experiments. Moreover, it was capable of colonizing and affecting olive blossoms. Further analyses in olive oil extracted from fruits artificially inoculated with F. solani indicated a significant increase in oil acidity, K232, K270 and peroxide value, whereas total phenols content was significantly decreased. To the best of our knowledge, this is the first report of Fusarium solani associated with olive fruit rot and olive oil degradation worldwide.

ACS Style

Emmanouil Alexandros Markakis; Emmanouil N. Roditakis; Georgios S. Kalantzakis; Anastasia Chatzaki; Stefanos K. Soultatos; Marianna Stavrakaki; Georgia I. Tavlaki; George C. Koubouris; Nikolaos Bagkis; Dimitrios E. Goumas. Characterization of fungi associated with olive fruit rot and olive oil degradation in Crete, southern Greece. Plant Disease 2021, 1 .

AMA Style

Emmanouil Alexandros Markakis, Emmanouil N. Roditakis, Georgios S. Kalantzakis, Anastasia Chatzaki, Stefanos K. Soultatos, Marianna Stavrakaki, Georgia I. Tavlaki, George C. Koubouris, Nikolaos Bagkis, Dimitrios E. Goumas. Characterization of fungi associated with olive fruit rot and olive oil degradation in Crete, southern Greece. Plant Disease. 2021; ():1.

Chicago/Turabian Style

Emmanouil Alexandros Markakis; Emmanouil N. Roditakis; Georgios S. Kalantzakis; Anastasia Chatzaki; Stefanos K. Soultatos; Marianna Stavrakaki; Georgia I. Tavlaki; George C. Koubouris; Nikolaos Bagkis; Dimitrios E. Goumas. 2021. "Characterization of fungi associated with olive fruit rot and olive oil degradation in Crete, southern Greece." Plant Disease , no. : 1.

Journal article
Published: 28 December 2020 in Sustainability
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Detailed knowledge of soil properties is fundamentally important for optimizing agriculture practices and management. Meanwhile, the spatial distribution of soil physicochemical properties is considered a fundamental input of any sustainable agricultural planning. In the present study, ordinary kriging, regression kriging and IDW were chosen for deciphering soil spatial variability and mapping soil properties in a reclaimed area of the Behera Governorate of Egypt where soil arose from two different types, one sandstone and the other limestone. Geostatistics were used to show the interrelationships and conditions of soil properties (available phosphorus, potassium and nitrogen, EC, pH, Sp, ESP, CEC, OC, SAR, and CaCO3). The results of mapping spatial soil variability by Geostatistics could be used for precision agriculture applications. Based on the soil test results, nutrient management recommendations should be applied regarding variable rates of fertilizers. The performance of the maps was evaluated using Mean square error (MSE). Inverse distance weight (IDW) showed higher efficiency than Kriging as a prediction method for mapping the studied soil properties in the study area. The results of the present study suggest that the application of the selected fit model worldwide in any relevant study of soil properties of different geological sources is feasible.

ACS Style

Mohamed A. E. AbdelRahman; Yasser M. Zakarya; Mohamed M. Metwaly; Georgios Koubouris. Deciphering Soil Spatial Variability through Geostatistics and Interpolation Techniques. Sustainability 2020, 13, 194 .

AMA Style

Mohamed A. E. AbdelRahman, Yasser M. Zakarya, Mohamed M. Metwaly, Georgios Koubouris. Deciphering Soil Spatial Variability through Geostatistics and Interpolation Techniques. Sustainability. 2020; 13 (1):194.

Chicago/Turabian Style

Mohamed A. E. AbdelRahman; Yasser M. Zakarya; Mohamed M. Metwaly; Georgios Koubouris. 2020. "Deciphering Soil Spatial Variability through Geostatistics and Interpolation Techniques." Sustainability 13, no. 1: 194.

Research article
Published: 07 December 2020 in International Journal of Remote Sensing
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Hyperspectral reflectance is becoming an increasingly popular source of information for plants’ physio-chemical status. In this study, net photosynthetic rate (A) and the activity of four antioxidant enzymes of Phaseolus vulgaris L., superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX), were compared with hyperspectral data using regression methods (principal component regression (PCR) and partial least squares regression (PLSR)) and vegetation indices (normalized difference vegetation index (NDVI), photochemical reflectance index (PRI), water index (WI) and the normalized reflectance (R) ratio R 710/R 750). The experiment was conducted on potted plants of two common bean genotypes (‘Blue lake’ and ‘Terli’), receiving four treatments (control, drought, salinity and drought + salinity), in an unheated greenhouse. The results suggest that both types of abiotic stress have a significant impact on the photosynthetic operation and the function of the antioxidant mechanism. The two regression methods performed well in predicting photosynthetic rate (A), while a strong correlation between A with the two vegetation indices was also revealed. Antioxidant enzymes appeared to be correlated with the WI (GPX and SOD enzymes) and the normalized reflectance (R) ratio R 710/R 750 (APX and SOD enzymes). Hyperspectral data seem to be particularly useful for fast and non-destructive assessment method for photosynthesis and enzymes activity of common bean plant.

ACS Style

Blagoja Boshkovski; Constantinos Tzerakis; Georgios Doupis; Anhelina Zapolska; Chariton Kalaitzidis; Georgios Koubouris. Relationship between physiological and biochemical measurements with spectral reflectance for two Phaseolus vulgaris L. genotypes under multiple stress. International Journal of Remote Sensing 2020, 42, 1230 -1249.

AMA Style

Blagoja Boshkovski, Constantinos Tzerakis, Georgios Doupis, Anhelina Zapolska, Chariton Kalaitzidis, Georgios Koubouris. Relationship between physiological and biochemical measurements with spectral reflectance for two Phaseolus vulgaris L. genotypes under multiple stress. International Journal of Remote Sensing. 2020; 42 (4):1230-1249.

Chicago/Turabian Style

Blagoja Boshkovski; Constantinos Tzerakis; Georgios Doupis; Anhelina Zapolska; Chariton Kalaitzidis; Georgios Koubouris. 2020. "Relationship between physiological and biochemical measurements with spectral reflectance for two Phaseolus vulgaris L. genotypes under multiple stress." International Journal of Remote Sensing 42, no. 4: 1230-1249.

Journal article
Published: 02 December 2020 in Sustainability
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Olive tree growth and reproduction are severely affected by temperature extremes, compromising fruit yield. In that aspect, the olive varieties “Koroneiki” and “Mastoidis” were employed in a mild cold stress experiment, imitating night frost incidents to assess their biochemical, physiological and reproductive functions in relation to genotype. The physiological performance of the stressed plants was not significantly altered, suggesting that both cultivars were well adapted to mild cold night stress. The biochemical response of the plants, regarding antioxidant enzymes, H2O2 and TBARS accumulation, confirmed that both cultivars could cope with the stress applied. The mRNA levels of the PPO gene, which participates in hydroxytyrosol biosynthesis and plant defense, were elevated after 24-h stress at 0 °C, in both cultivars with “Mastoidis” plants exhibiting higher levels for a longer period. Three more genes involved in hydroxytyrosol biosynthesis upregulated their expression levels as a response to cold stress. The numerous plant phenology aspects measured reinforced the conclusion that both cultivars responded to the stress applied. The results of the present study may contribute to better understanding olive tree adaptive responses to low temperature events, an abiotic stress condition that is often present in an open plantation, thus assisting farmers on breeding and cultivar selection.

ACS Style

Niki Mougiou; Boushra Baalbaki; Georgios Doupis; Nektarios Kavroulakis; Stylianos Poulios; Konstantinos Vlachonasios; Georgios Koubouris. The Effect of Low Temperature on Physiological, Biochemical and Flowering Functions of Olive Tree in Relation to Genotype. Sustainability 2020, 12, 10065 .

AMA Style

Niki Mougiou, Boushra Baalbaki, Georgios Doupis, Nektarios Kavroulakis, Stylianos Poulios, Konstantinos Vlachonasios, Georgios Koubouris. The Effect of Low Temperature on Physiological, Biochemical and Flowering Functions of Olive Tree in Relation to Genotype. Sustainability. 2020; 12 (23):10065.

Chicago/Turabian Style

Niki Mougiou; Boushra Baalbaki; Georgios Doupis; Nektarios Kavroulakis; Stylianos Poulios; Konstantinos Vlachonasios; Georgios Koubouris. 2020. "The Effect of Low Temperature on Physiological, Biochemical and Flowering Functions of Olive Tree in Relation to Genotype." Sustainability 12, no. 23: 10065.

Journal article
Published: 28 October 2020 in Agronomy
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During recent centuries, cultivated olive has evolved to one of the major tree crops in the Mediterranean Basin and lately expanded to America, Australia, and Asia producing an estimated global average value of over USD 18 billion. A long-term research effort has been established with the long-term goal to preserve biodiversity, characterize agronomic behavior, and ultimately utilize genotypes suitable for cultivation in areas of unfavorable environmental conditions. In the present study, a combination of 10 simple sequence repeat (SSR) markers with the classification binary tree (CBT) analysis was evaluated as a method for discriminating genotypes within cultivated olive trees, while Olea europaea subsp. cuspidata was also used as an outgroup. The 10 SSR loci employed in this study, were highly polymorphic and gave reproducible amplification patterns for all accessions analyzed. Genetic analysis indicated that the group of SSR loci employed was highly informative. A further analysis revealed that two sub populations and pairwise relatedness gave insight about synonymies. In conclusion, the CBT method which employed SSR allelic sizes proved to be a valuable tool in order to distinguish olive cultivars over the traditional unweighted pair group method with the arithmetic mean (UPGMA) algorithm. Further research which will combine phenotyping characterization of olive germplasm will have the potential to enable the utilization of existing, and breeding of new, superior cultivars.

ACS Style

Evangelia V. Avramidou; Georgios C. Koubouris; Panos V. Petrakis; Katerina K. Lambrou; Ioannis T. Metzidakis; Andreas G. Doulis. Classification Binary Trees with SSR Allelic Sizes: Combining Regression Trees with Genetic Molecular Data in Order to Characterize Genetic Diversity between Cultivars of Olea europaea L. Agronomy 2020, 10, 1662 .

AMA Style

Evangelia V. Avramidou, Georgios C. Koubouris, Panos V. Petrakis, Katerina K. Lambrou, Ioannis T. Metzidakis, Andreas G. Doulis. Classification Binary Trees with SSR Allelic Sizes: Combining Regression Trees with Genetic Molecular Data in Order to Characterize Genetic Diversity between Cultivars of Olea europaea L. Agronomy. 2020; 10 (11):1662.

Chicago/Turabian Style

Evangelia V. Avramidou; Georgios C. Koubouris; Panos V. Petrakis; Katerina K. Lambrou; Ioannis T. Metzidakis; Andreas G. Doulis. 2020. "Classification Binary Trees with SSR Allelic Sizes: Combining Regression Trees with Genetic Molecular Data in Order to Characterize Genetic Diversity between Cultivars of Olea europaea L." Agronomy 10, no. 11: 1662.

Journal article
Published: 17 May 2020 in South African Journal of Botany
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Soil salinization is considered as one of the most important risks for agricultural soils. The objective of this experiment was to study the ecophysiological and the biochemical behaviour of two cultivars of Olea europaea L., 'Chemlali' and 'Koroneiki' under two salinity levels (100 and 200 mM NaCl) and the potential alleviation induced by gibberellic acid (GA3) foliar sprays. Salinity treatments significantly decreased photosynthetic assimilation rate and stomatal conductance compared to the control for both cultivars, but ‘Chemlali’ showed a higher resistance to increasing NaCl salinity compared to 'Koroneiki'. Leaf chlorophyll index also reduced gradually with increasing salinity concentration compared to the control. At the end of the experiment, a decrease in growth and dry matter accumulation was observed. Under high salinity stress, a significant decrease in root DW was recorded by 37% and 59% for ‘Chemlali’ and ‘Koroneiki’, respectively. High salinity stress decreased also shoot DW up to 51% for ‘Chemlali’. However, mannitol concentration increased under increasing salinity levels compared to control for ‘Chemlali’ cultivars. Interestingly, foliar application of GA3 alleviated the negative effects of salinity on ecophysiological parameters especially for ‘Koroneiki’. Indeed, GA3 improved photosynthetic assimilation up to 14% for ‘Chemlali’ and 36% for ‘Koroneiki’ compared to high salinity treatment. Both cultivars showed an increase in leaf chlorophyll index after applying GA3. Under high salinity combined with GA3, growth and dry weight were increased compared to salt stressed plants without GA3. The obtained results report that ‘Chemlali’ cultivar is more tolerant to salinity than ‘Koroneiki’ and suggest that GA3 plays an important role to reduce negative effects of NaCl salinity.

ACS Style

Ines Moula; Olfa Boussadia; Georgios Koubouris; Mortadha Ben Hassine; Wafa Boussetta; Marie Christine Van Labeke; Mohamed Braham. Ecophysiological and biochemical aspects of olive tree (Olea europaea L.) in response to salt stress and gibberellic acid-induced alleviation. South African Journal of Botany 2020, 132, 38 -44.

AMA Style

Ines Moula, Olfa Boussadia, Georgios Koubouris, Mortadha Ben Hassine, Wafa Boussetta, Marie Christine Van Labeke, Mohamed Braham. Ecophysiological and biochemical aspects of olive tree (Olea europaea L.) in response to salt stress and gibberellic acid-induced alleviation. South African Journal of Botany. 2020; 132 ():38-44.

Chicago/Turabian Style

Ines Moula; Olfa Boussadia; Georgios Koubouris; Mortadha Ben Hassine; Wafa Boussetta; Marie Christine Van Labeke; Mohamed Braham. 2020. "Ecophysiological and biochemical aspects of olive tree (Olea europaea L.) in response to salt stress and gibberellic acid-induced alleviation." South African Journal of Botany 132, no. : 38-44.

Articles
Published: 19 April 2020 in International Journal of Remote Sensing
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Fomitiporia mediterranea, commonly known as ‘Esca’, is a detrimental fungus for many tree species and grapevine and is considered to be one of the main causal agents of wood decay of olive plantations in the Mediterranean region. Symptomatic trees are mainly identified at the advanced stages of the disease, so no curative control measures can be applied. In recent years, spectral measurements have been used in agriculture for early detection of disease incidents. In this paper, Linear Discriminant Analysis (LDA) of hyperspectral data collected in situ by a ASD FieldSpec® 3 spectroradiometer was used to investigate its potential for identifying alterations caused by the fungus in the most popular Greek olive cultivars ‘Amfissis’, ‘Chalkidikis’, ‘Mastoidis’, ‘Koroneiki’ and ‘Kalamon’. In order to identify the optimal wavelength ranges for LDA that are indicative of Fomitiporia mediterranea presence, Principal Component Regression (PCR) and Partial Least Square Regression (PLSR) were applied. The results showed a good classification accuracy of infected and non-infected trees. Furthermore, trees that were not identified as diseased through laboratory analysis (reisolation from trunks) but had been artificially infected at the beginning of the experiment, were actually classified as infected by LDA, implying that hyperspectral scanning was able to identify past infection even if the pathogen was no longer present.

ACS Style

Anhelina Zapolska; Chariton Kalaitzidis; Emmanouil Markakis; Eleftherios Ligoxigakis; Georgios Koubouris. Linear Discriminant Analysis of spectral measurements for discrimination between healthy and diseased trees of Olea europaea L. artificially infected by Fomitiporia mediterranea. International Journal of Remote Sensing 2020, 41, 5388 -5398.

AMA Style

Anhelina Zapolska, Chariton Kalaitzidis, Emmanouil Markakis, Eleftherios Ligoxigakis, Georgios Koubouris. Linear Discriminant Analysis of spectral measurements for discrimination between healthy and diseased trees of Olea europaea L. artificially infected by Fomitiporia mediterranea. International Journal of Remote Sensing. 2020; 41 (14):5388-5398.

Chicago/Turabian Style

Anhelina Zapolska; Chariton Kalaitzidis; Emmanouil Markakis; Eleftherios Ligoxigakis; Georgios Koubouris. 2020. "Linear Discriminant Analysis of spectral measurements for discrimination between healthy and diseased trees of Olea europaea L. artificially infected by Fomitiporia mediterranea." International Journal of Remote Sensing 41, no. 14: 5388-5398.

Journal article
Published: 11 April 2020 in Climate
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Olive cultivation is considered as one of the most significant agricultural activities in Greece, from a financial, social, and ecological point of view. Intensive cultivation practices in combination with the Mediterranean climate, lead to depletion of soil organic matter, erosion, desertification, and degradation of water resources. This paper describes sustainable olive crop management practices that were comparatively applied in 120 olive groves in Greece for 5 years with the participation of three farmers groups. Organic materials recycled in the olive groves during the present study were valuable sources of carbon, nitrogen, phosphorus, and potassium. Carbon content was highest in pruning residue (53.8–54.2%) while all materials studied were considered rich in C ranging between 41.9–46.2% (compost) and 34.9–42.5% (three-phase olive mill waste-OMW). The highest content in nitrogen was detected in compost (2–2.45%) followed by pruning residue (0.93–0.99%) and OMW (0.03–0.1%). Compost was considered a good source of phosphorus (0.3–0.6%) followed by pruning residue (0.08–0.13%) and OMW (0.01–0.3%). Potassium was also considerable in the organic materials recycled ranging 0.5–1.5% in compost followed by pruning residue (0.5–0.7%) and OMW (0.3–1.1%). Adoption of modified pruning also had important contribution toward sustainable management of olive trees. Sustainable pruning resulted in a well-balanced ratio between vegetative growth and fruiting (balanced, every year, in order to eradicate biennial bearing). Significant fluctuation in olive yields was observed in the first years of the project while yields were gradually stabilised by applying sustainable crop management. In parallel, yield increase without additional inputs, lowers the carbon—environmental footprint of the product regarding several environmental impact categories. Results can be integrated in the national agricultural and environmental policy in Mediterranean countries toward the achievement of a circular economy.

ACS Style

G. Michalopoulos; K. A. Kasapi; G. Koubouris; G. Psarras; G. Arampatzis; E. Hatzigiannakis; V. Kavvadias; C. Xiloyannis; G. Montanaro; S. Malliaraki; A. Angelaki; C. Manolaraki; G. Giakoumaki; S. Reppas; N. Kourgialas; G. Kokkinos. Adaptation of Mediterranean Olive Groves to Climate Change through Sustainable Cultivation Practices. Climate 2020, 8, 54 .

AMA Style

G. Michalopoulos, K. A. Kasapi, G. Koubouris, G. Psarras, G. Arampatzis, E. Hatzigiannakis, V. Kavvadias, C. Xiloyannis, G. Montanaro, S. Malliaraki, A. Angelaki, C. Manolaraki, G. Giakoumaki, S. Reppas, N. Kourgialas, G. Kokkinos. Adaptation of Mediterranean Olive Groves to Climate Change through Sustainable Cultivation Practices. Climate. 2020; 8 (4):54.

Chicago/Turabian Style

G. Michalopoulos; K. A. Kasapi; G. Koubouris; G. Psarras; G. Arampatzis; E. Hatzigiannakis; V. Kavvadias; C. Xiloyannis; G. Montanaro; S. Malliaraki; A. Angelaki; C. Manolaraki; G. Giakoumaki; S. Reppas; N. Kourgialas; G. Kokkinos. 2020. "Adaptation of Mediterranean Olive Groves to Climate Change through Sustainable Cultivation Practices." Climate 8, no. 4: 54.

Articles
Published: 16 February 2020 in Communications in Soil Science and Plant Analysis
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Salinity and drought stresses are critical for Phaseolus vulgaris L. growth and development. They affect plants in various ways, including tissue mineral element content. Micro- and macro-elements in leaves of Phaseolus vulgaris L. (cv. ‘Blue lake’ and cv. ‘Terli’) subjected to deficit irrigation and salinity treatments were investigated, both analytically and with regards to their effect on the leaves’ spectral reflectance. B (boron), K (potassium), Mn (manganese), Na (sodium), Si (silicon) and Zn (zinc) appeared to be influenced by stress factors, mainly responding to salinity increase. The leaf spectral reflectance of the plants appeared to be significantly correlated with most of the elements under investigation. Multivariate regression identified a relationship of the reflectance at particular regions of the spectrum with phosphorus and NDVI (normalized difference vegetation index) and indicated a significant correlation with B, Fe (iron), K, Mn, P (phosphorus) and Zn. Moreover, customized spectral indices, exhibiting significantly high correlation with B, Fe, K, Mg (magnesium), Mn, Na, P, Zn and N (nitrogen), were developed.

ACS Style

Blagoja Boshkovski; Constantinos Tzerakis; Georgios Doupis; Anhelina Zapolska; Chariton Kalaitzidis; Georgios Koubouris. Relationships of Spectral Reflectance with Plant Tissue Mineral Elements of Common Bean (Phaseolus Vulgaris L.) Under Drought and Salinity Stresses. Communications in Soil Science and Plant Analysis 2020, 51, 675 -686.

AMA Style

Blagoja Boshkovski, Constantinos Tzerakis, Georgios Doupis, Anhelina Zapolska, Chariton Kalaitzidis, Georgios Koubouris. Relationships of Spectral Reflectance with Plant Tissue Mineral Elements of Common Bean (Phaseolus Vulgaris L.) Under Drought and Salinity Stresses. Communications in Soil Science and Plant Analysis. 2020; 51 (5):675-686.

Chicago/Turabian Style

Blagoja Boshkovski; Constantinos Tzerakis; Georgios Doupis; Anhelina Zapolska; Chariton Kalaitzidis; Georgios Koubouris. 2020. "Relationships of Spectral Reflectance with Plant Tissue Mineral Elements of Common Bean (Phaseolus Vulgaris L.) Under Drought and Salinity Stresses." Communications in Soil Science and Plant Analysis 51, no. 5: 675-686.

Journal article
Published: 01 July 2019 in Acta Horticulturae
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ACS Style

O.A. Fakher; G. Doupis; G. Psarras; Androniki Papafilippaki; Georgios Koubouris. Evaluation of legume-cereal seed mixtures for enhancement of soil fertility, carbon storage and biodiversity in a low-intensity olive orchard. Acta Horticulturae 2019, 309 -314.

AMA Style

O.A. Fakher, G. Doupis, G. Psarras, Androniki Papafilippaki, Georgios Koubouris. Evaluation of legume-cereal seed mixtures for enhancement of soil fertility, carbon storage and biodiversity in a low-intensity olive orchard. Acta Horticulturae. 2019; (1242):309-314.

Chicago/Turabian Style

O.A. Fakher; G. Doupis; G. Psarras; Androniki Papafilippaki; Georgios Koubouris. 2019. "Evaluation of legume-cereal seed mixtures for enhancement of soil fertility, carbon storage and biodiversity in a low-intensity olive orchard." Acta Horticulturae , no. 1242: 309-314.

Journal article
Published: 27 June 2019 in Biologia plantarum
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ACS Style

Georgios Koubouris; I. Limperaki; M. Darioti; C. Sergentani. Effects of various winter chilling regimes on flowering quality indicatorsof Greek olive cultivars. Biologia plantarum 2019, 1 .

AMA Style

Georgios Koubouris, I. Limperaki, M. Darioti, C. Sergentani. Effects of various winter chilling regimes on flowering quality indicatorsof Greek olive cultivars. Biologia plantarum. 2019; ():1.

Chicago/Turabian Style

Georgios Koubouris; I. Limperaki; M. Darioti; C. Sergentani. 2019. "Effects of various winter chilling regimes on flowering quality indicatorsof Greek olive cultivars." Biologia plantarum , no. : 1.

Original article
Published: 16 March 2019 in Tree Genetics & Genomes
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Seed (endocarp) morphology is useful for genotype discrimination and cultivar classification. Over a 20-year period, 504 olive trees (Olea europaea subsp. europaea) previously assigned to different cultivars originating from Greece (n = 37), Spain (n = 2), and Italy (n = 2) as well as one accession of Olea europaea subsp. cuspidata were evaluated employing 11 endocarp morphological markers and 9 SSR markers. A matrix of 42 morphotypes in total was subjected to classification binary tree (CBT) analysis. In addition, cultivars were fingerprinted employing 9 microsatellite (SSR) markers and placed on a similarity dendrogram. All 41 olive cultivars and one accession of Olea europaea subsp. cuspidata employed in the present study yielded different morphological profiles produced by the 11 endocarp traits. In the resulting CBT, the total reduction in error, that is, the total information in the set of all 42 morphotypes, was 100%. This meant that the set of 11 morphological characters—having 28 different states in all—was sufficient to remove all data noise and to correctly classify all examined olive cultivars. In addition, all olive cultivars were successfully discriminated by the 9 SSR markers employed. It is suggested that cultivars with large seeds—and concomitantly large fruits—are more distant from the wild forms and probably more evolved compared to cultivars with small seeds. In corroboration to the above, based on seed shape, some of the olive cultivars showed high resemblance to wild olives leading thus to the hypothesis that they were produced or selected during the early ages of olive domestication.

ACS Style

Georgios Koubouris; Evangelia Avramidou; I. T. Metzidakis; P. V. Petrakis; C. K. Sergentani; A. G. Doulis. Phylogenetic and evolutionary applications of analyzing endocarp morphological characters by classification binary tree and leaves by SSR markers for the characterization of olive germplasm. Tree Genetics & Genomes 2019, 15, 26 .

AMA Style

Georgios Koubouris, Evangelia Avramidou, I. T. Metzidakis, P. V. Petrakis, C. K. Sergentani, A. G. Doulis. Phylogenetic and evolutionary applications of analyzing endocarp morphological characters by classification binary tree and leaves by SSR markers for the characterization of olive germplasm. Tree Genetics & Genomes. 2019; 15 (2):26.

Chicago/Turabian Style

Georgios Koubouris; Evangelia Avramidou; I. T. Metzidakis; P. V. Petrakis; C. K. Sergentani; A. G. Doulis. 2019. "Phylogenetic and evolutionary applications of analyzing endocarp morphological characters by classification binary tree and leaves by SSR markers for the characterization of olive germplasm." Tree Genetics & Genomes 15, no. 2: 26.

Chapter
Published: 08 March 2019 in Soil Fertility Management for Sustainable Development
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Olive (Olea europaea L.) is a common cultivated tree crop in the Mediterranean Basin. Inappropriate cultivation practices (i.e. excessive tillage and application of herbicides, the absence of organic amendments, the burning of pruning residues in situ) lead, in combination with the Mediterranean climate, to the depletion of soil organic matter, erosion, desertification and degradation of water resources. Strategies based on changes in the land management (e.g. cover crops and green manure, restriction of tillage, recycling of agricultural wastes and pruning residues), have been reported to enhance soil structure, increase soil fertility, decrease soil erosion, increase the C stored in soil, and reduce atmospheric CO2. This chapter highlights soil management techniques that could promote the conservation of the productive olive grove system and thus contribute to the sustainability of the natural resources. During olive tree growth and olive oil production a large quantity of plant residues and mill wastes are produced. The implementation of alternative olive cultivation techniques (reduced/no tillage, plant residue and weed management, tree pruning, etc.) has not been systematically tested under the prevailing conditions of the Mediterranean olive forest. Although there are multiple specific studies taking into account specific practices in selected regions, a holistic approach for the Mediterranean olive groves has not yet been known. The design, the development and the adoption of an integrated soil management system in olive groves, adjustable to local soil climatic conditions is proposed, among further research priorities.

ACS Style

Victor Kavvadias; Georgios Koubouris. Sustainable Soil Management Practices in Olive Groves. Soil Fertility Management for Sustainable Development 2019, 167 -188.

AMA Style

Victor Kavvadias, Georgios Koubouris. Sustainable Soil Management Practices in Olive Groves. Soil Fertility Management for Sustainable Development. 2019; ():167-188.

Chicago/Turabian Style

Victor Kavvadias; Georgios Koubouris. 2019. "Sustainable Soil Management Practices in Olive Groves." Soil Fertility Management for Sustainable Development , no. : 167-188.

Research paper
Published: 17 December 2018 in Plant Biology
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Vitamin E is a general term used to describe a group of eight lipophilic compounds known as tocochromanols. These vitamin E variants are chemically categorized into two classes formed by α‐, β‐, γ‐, and δ‐ tocopherols and tocotrienols isoforms, respectively. The present study describes the concurrent regulation of genes and metabolites orchestrating vitamin E biosynthesis in olive drupes of five distinctive Greek olive cultivars. A combination of physiological, analytical, biochemical and molecular approaches was employed in order to carry out comparative analyses including real‐time RT‐qPCR for gene expression levels and HPLC analysis of metabolite contents. Findings indicated that tocochromanol levels and composition, oil content, gene expression levels as well as total antioxidant activity were highly dependent on cultivar genotype and, to a lesser extent, on fruit developmental stage. Specifically, cultivars ‘Kalokairida’ and ‘Lianolia Kerkyras’ demonstrated the highest vitamin E content, with cv. ‘Lianolia Kerkyras’ showing high tocochromanol content combined with highest overall antioxidant activity in all developmental stages, concomitant with the up‐regulation expression profile of HPPD. The genotypic imprint versus the temporal contribution to vitamin E levels, as well as the potential link to lipid peroxidation amelioration are discussed.

ACS Style

Egli C. Georgiadou; Georgios Koubouris; Vlasios Goulas; Chrysi Sergentani; Nikolaos Nikoloudakis; George A. Manganaris; Panagiotis Kalaitzis; Vasileios Fotopoulos. Genotype‐dependent regulation of vitamin E biosynthesis in olive fruits as revealed through metabolic and transcriptional profiles. Plant Biology 2018, 21, 604 -614.

AMA Style

Egli C. Georgiadou, Georgios Koubouris, Vlasios Goulas, Chrysi Sergentani, Nikolaos Nikoloudakis, George A. Manganaris, Panagiotis Kalaitzis, Vasileios Fotopoulos. Genotype‐dependent regulation of vitamin E biosynthesis in olive fruits as revealed through metabolic and transcriptional profiles. Plant Biology. 2018; 21 (4):604-614.

Chicago/Turabian Style

Egli C. Georgiadou; Georgios Koubouris; Vlasios Goulas; Chrysi Sergentani; Nikolaos Nikoloudakis; George A. Manganaris; Panagiotis Kalaitzis; Vasileios Fotopoulos. 2018. "Genotype‐dependent regulation of vitamin E biosynthesis in olive fruits as revealed through metabolic and transcriptional profiles." Plant Biology 21, no. 4: 604-614.

Journal article
Published: 01 December 2018 in Journal of Water and Climate Change
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Due to global population growth, there is an increasing demand for food production. Consumers are informed about positive (stronger immune system through vitamins) and negative (cancer through pesticide residue) effects of nutrition, and there are also many health-related dietary requirements (e.g. lactose or gluten intolerance, diabetes, etc.) that result in the need for high quality and safe food. Another challenge for agriculture is adaptation to climate change (CC) especially in areas with intense competition for natural resources between agriculture and other uses, or with sensitivity to environmental degradation such as soil erosion, desertification or salinization.

ACS Style

Georgios Koubouris. Advances in prediction, monitoring and mitigation of climate change effects on water resources and good agricultural practices for crop adaptation to environmental stresses. Journal of Water and Climate Change 2018, 9, 631 -632.

AMA Style

Georgios Koubouris. Advances in prediction, monitoring and mitigation of climate change effects on water resources and good agricultural practices for crop adaptation to environmental stresses. Journal of Water and Climate Change. 2018; 9 (4):631-632.

Chicago/Turabian Style

Georgios Koubouris. 2018. "Advances in prediction, monitoring and mitigation of climate change effects on water resources and good agricultural practices for crop adaptation to environmental stresses." Journal of Water and Climate Change 9, no. 4: 631-632.

Journal article
Published: 09 November 2018 in Science of The Total Environment
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Water scarcity in the Mediterranean region is becoming a growing concern, threatening the viability of agriculture, which is one of the main economic sectors in many areas. The design of an optimal irrigation management plan, based on state-of-the-art measuring and modeling tools, can effectively contribute towards water saving efforts and potentially address the water scarcity issue in the region. This paper describes the development and application of an integrated decision-making system for the management of water resources of olive and citrus crops in the North of Chania, Crete, Greece. The system integrates different field measurements, for example 2088 soil moisture measurements taken within the study area, and modeling approaches to simulate flow in the unsaturated zone. After the successful calibration and validation of the model, the spatio-temporal representation of soil moisture and pore water pressure were used as guidance for developing optimal irrigation plans, taking into account the water needs of olive and citrus crops, aiming to maximize crop yield, agricultural income, and promote water saving efforts. According to the results, water use can be reduced by up to 36% during the dry season, compared to conventional irrigation practices for citrus trees. Similarly, for olive trees, the reduction in water use can reach up to 41%. The proposed methodology can also be cost-effective in terms of water value, saving about 40% from the typical water cost for irrigation in the study area. The impact of climate change on water resources availability in the area and water conservation efforts were also investigated for the period of (2019–2030). Results show that, comparing the Baseline, RCP 8.5 and RCP 4.5 climatic scenarios, the highest savings on average are observed for emission scenario RCP 4.5 with 53.3% water savings for olive trees and 46.7% for citrus trees.

ACS Style

Nektarios N. Kourgialas; Georgios Koubouris; Zoi Dokou. Optimal irrigation planning for addressing current or future water scarcity in Mediterranean tree crops. Science of The Total Environment 2018, 654, 616 -632.

AMA Style

Nektarios N. Kourgialas, Georgios Koubouris, Zoi Dokou. Optimal irrigation planning for addressing current or future water scarcity in Mediterranean tree crops. Science of The Total Environment. 2018; 654 ():616-632.

Chicago/Turabian Style

Nektarios N. Kourgialas; Georgios Koubouris; Zoi Dokou. 2018. "Optimal irrigation planning for addressing current or future water scarcity in Mediterranean tree crops." Science of The Total Environment 654, no. : 616-632.

Article
Published: 30 October 2018 in European Journal of Plant Pathology
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Wood decay caused primarily by Fomitiporia mediterranea is considered to be an emerging disease for citrus and olive trees in Mediterranean countries. The pathogen cannot be currently controlled by chemicals whereas there is no available information about the source and level of resistance even for the most important cultivars where the global citrus and olive industries are based on. In the present study, five of the most significant Greek olive cultivars (Amfissis, Chalkidikis, Kalamon, Koroneiki and Mastoidis) were evaluated for their resistance to F. mediterranea by conducting artificial inoculation experiments. Disease reactions were evaluated by measuring the length of bark and wood lesions, 33 months post inoculation. Trunk sections scanning and image pixel analysis were employed to estimate the total discoloration and decay area of infected wood, whereas positive re-isolation ratio of F. mediterranea was also taken into account in resistance evaluation. Data on symptom development along with wood tissue colonization by the pathogen indicated that the resistance of olive cultivars to F. mediterranea varied significantly. Based on the cumulative stress response index (CSRI), ‘Kalamon’ and ‘Koroneiki’ were classified as resistant, ‘Chalkidikis’ and ‘Mastoidis’ as intermediately resistant, whereas ‘Amfissis’ was susceptible. Determination of lignin in trunk wood showed that the resistance of olive cultivars to F. mediterranea could be associated with their lignin content. This is the first experimental evidence of differential susceptibility responses of olive cultivars against F. mediterranea, and reveals for the first time the potential exploitation of host resistance as a promising approach in the effort to control wood decay disease of olive in practice.

ACS Style

Emmanouil A. Markakis; Eleftherios K. Ligoxigakis; Peter A. Roussos; Chrysi K. Sergentani; Nektarios Kavroulakis; Emmanouil N. Roditakis; Georgios C. Koubouris. Differential susceptibility responses of Greek olive cultivars to Fomitiporia mediterranea. European Journal of Plant Pathology 2018, 153, 1055 -1066.

AMA Style

Emmanouil A. Markakis, Eleftherios K. Ligoxigakis, Peter A. Roussos, Chrysi K. Sergentani, Nektarios Kavroulakis, Emmanouil N. Roditakis, Georgios C. Koubouris. Differential susceptibility responses of Greek olive cultivars to Fomitiporia mediterranea. European Journal of Plant Pathology. 2018; 153 (4):1055-1066.

Chicago/Turabian Style

Emmanouil A. Markakis; Eleftherios K. Ligoxigakis; Peter A. Roussos; Chrysi K. Sergentani; Nektarios Kavroulakis; Emmanouil N. Roditakis; Georgios C. Koubouris. 2018. "Differential susceptibility responses of Greek olive cultivars to Fomitiporia mediterranea." European Journal of Plant Pathology 153, no. 4: 1055-1066.

Journal article
Published: 03 September 2018 in Journal of Water and Climate Change
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Olive trees constitute one of the most dynamic cultivations for Mediterranean countries, while their economic importance is high. As water constitutes a fundamental factor affecting olive tree production, soil water content is a most critical parameter that must be monitored to improve olive trees’ cultivation management. Effects of precipitation, irrigation, and soil management on water content in four soil depths (10, 20, 30, 40 cm), four periods of the year (February–March, April–May, June–July, August–September) and three successive years were determined in 12 Mediterranean olive groves (Trifilia, southern Greece) as well as their respective fruit and olive oil yields. Significantly higher soil water content was recorded in the first (+16.8%) and third (+27.4%) year compared to the second year. Higher (+6.8%) water content was observed in irrigated olive groves compared to rainfed fields. Higher (+5.6%) water content was observed in sustainable olive groves compared to intensively managed fields. Significantly, higher soil moisture was recorded at 40 and 30 cm depth compared to 10 cm depth while intermediate values were observed at 20 cm. Marked increase in fruit yield was achieved through sustainable management (+39%) compared to intensive olive groves. The potential to improve irrigation practices in the area was also indicated by results of the present study.

ACS Style

G. Arampatzis; E. Hatzigiannakis; V. Pisinaras; N. Kourgialas; G. Psarras; V. Kinigopoulou; A. Panagopoulos; Georgios Koubouris. Soil water content and olive tree yield responses to soil management, irrigation, and precipitation in a hilly Mediterranean area. Journal of Water and Climate Change 2018, 9, 672 -678.

AMA Style

G. Arampatzis, E. Hatzigiannakis, V. Pisinaras, N. Kourgialas, G. Psarras, V. Kinigopoulou, A. Panagopoulos, Georgios Koubouris. Soil water content and olive tree yield responses to soil management, irrigation, and precipitation in a hilly Mediterranean area. Journal of Water and Climate Change. 2018; 9 (4):672-678.

Chicago/Turabian Style

G. Arampatzis; E. Hatzigiannakis; V. Pisinaras; N. Kourgialas; G. Psarras; V. Kinigopoulou; A. Panagopoulos; Georgios Koubouris. 2018. "Soil water content and olive tree yield responses to soil management, irrigation, and precipitation in a hilly Mediterranean area." Journal of Water and Climate Change 9, no. 4: 672-678.

Journal article
Published: 03 September 2018 in Journal of Water and Climate Change
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The objective of this work was to classify and map the areas environmentally sensitive to desertification in the Mediterranean island of Crete. Sensitivity to desertification was estimated with a modification of the MEDALUS Environmentally Sensitive Area Index (ESAI) approach, using 15 quantitative parameters divided into four main quality indices: climate, vegetation, soils and management quality. The ESAI methodology was modified to include two additional parameters related to soil quality (water erosion and soil organic matter). According to the results, 37% of the island's area is characterized as critically sensitive to desertification. This percentage varies significantly across the island, with the western part having the least critically sensitive areas, and the eastern part the most critically sensitive. The results of this study also indicate that critically sensitive areas are found in the eastern side of the island mainly due to human-related factors and climatic conditions. It was concluded that the proposed methodology is a valuable tool for regional-scale assessment of areas environmentally sensitive to desertification in Mediterranean environments.

ACS Style

G. G. Morianou; N. N. Kourgialas; G. Psarras; Georgios Koubouris. Mapping sensitivity to desertification in Crete (Greece), the risk for agricultural areas. Journal of Water and Climate Change 2018, 9, 691 -702.

AMA Style

G. G. Morianou, N. N. Kourgialas, G. Psarras, Georgios Koubouris. Mapping sensitivity to desertification in Crete (Greece), the risk for agricultural areas. Journal of Water and Climate Change. 2018; 9 (4):691-702.

Chicago/Turabian Style

G. G. Morianou; N. N. Kourgialas; G. Psarras; Georgios Koubouris. 2018. "Mapping sensitivity to desertification in Crete (Greece), the risk for agricultural areas." Journal of Water and Climate Change 9, no. 4: 691-702.

Journal article
Published: 10 August 2018 in Journal of Water and Climate Change
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Olive trees are a major source of agricultural residues. Strategies based on different management of organic amendments have been reported to increase soil fertility. The effect of sustainable organic matter input practices (application of shredded pruning residue and olive residue compost to soil) on soil properties in irrigated and rain-fed olive groves was investigated. The study took place in 40 olive groves in the region of Peza, island of Crete, Greece during a 5-year period (2012–2017). The results showed that olive trees play an important role in soil nutrient conservation under semi-arid conditions in the Mediterranean basin. The addition of olive tree residues, in combination with conservation tillage practices, improved soil fertility over the experimental period. Most of the soil properties were favored by irrigation. In olive soil parcels receiving organic materials the soil organic matter and the total nitrogen were increased in irrigated fields. The ability of surface soil to sequester carbon and nutrients beneath the tree canopy of olive groves was high. It is recommended that sustainable soil management practices should consider soil fertility variability of olive orchards.

ACS Style

V. Kavvadias; M. Papadopoulou; E. Vavoulidou; S. Theocharopoulos; Georgios Koubouris; G. Psarras; Chr. Manolaraki; G. Giakoumaki; A. Vasiliadis. Effect of sustainable management of olive tree residues on soil fertility in irrigated and rain-fed olive orchards. Journal of Water and Climate Change 2018, 9, 764 -774.

AMA Style

V. Kavvadias, M. Papadopoulou, E. Vavoulidou, S. Theocharopoulos, Georgios Koubouris, G. Psarras, Chr. Manolaraki, G. Giakoumaki, A. Vasiliadis. Effect of sustainable management of olive tree residues on soil fertility in irrigated and rain-fed olive orchards. Journal of Water and Climate Change. 2018; 9 (4):764-774.

Chicago/Turabian Style

V. Kavvadias; M. Papadopoulou; E. Vavoulidou; S. Theocharopoulos; Georgios Koubouris; G. Psarras; Chr. Manolaraki; G. Giakoumaki; A. Vasiliadis. 2018. "Effect of sustainable management of olive tree residues on soil fertility in irrigated and rain-fed olive orchards." Journal of Water and Climate Change 9, no. 4: 764-774.