Colistin, a last resort antibiotic, is important for controlling infections with carbapenem-resistant Enterobacteriaceae. The recent emergence of mobile-colistin-resistance (mcr) genes has threatened the effectiveness of colistin. Aquaculture is hypothesized to be a major contributor to the evolution and dissemination of mcr. However, data on mcr in aquaculture are limited. Here, the occurrence of mcr-1 was assessed in Rainbow Trout in Lebanon, a country with developing antimicrobial stewardship and an established use of colistin for medical and farming purposes. mcr-1 was detected in 5 Escherichia coli isolated from fish guts. The isolates were classified as multidrug-resistant and their colistin minimum inhibitory concentration ranged between 16 and 32 μg/mL. Whole genome sequencing analysis showed that mcr-1 was carried on transmissible IncX4 plasmids and that the isolates harbored more than 14 antibiotic resistance genes. The isolates belonged to ST48 and ST101, which have been associated with mcr and can occur in humans and fish. The mcr-1-positive E. coli persisted in 6-day biofilms, but there was a potential fitness cost. Given the status of infrastructure in Lebanon, there is a high potential for the dissemination of mcr via aquatic environments. Urgent actions are needed to control mcr and to enhance antimicrobial stewardship in Lebanon.

Background/Objectives: Vitamin D—crucial for bone health, immune function, and hormone regulation—is deficient worldwide, affecting around half the population, particularly women. The study aims to determine the prevalence and risk factors of vitamin D deficiency and hypovitaminosis D in non-pregnant women in Lebanon. Methods: A national cross-sectional survey sampled households across Lebanon, covering 2803 non-pregnant women aged 15 to 49. Demographic information and dietary habits were collected, and anthropometric measurements and serum analyses, including 25-hydroxyvitamin D (25(OH)D) concentrations, were conducted. Multivariable Poisson regressions were constructed to calculate the adjusted prevalence ratio (aPR) for vitamin D deficiency and hypovitaminosis D of variables. Results: The prevalence of vitamin D deficiency (<30 nmol/L) among non-pregnant women in Lebanon was 37.9%, while 69.2% had hypovitaminosis D (<50 nmol/L). Wearing a veil (hijab) was identified as the most significant risk factor for both vitamin D deficiency (aPR = 3.76) and hypovitaminosis D (aPR = 1.47). Additionally, olive skin and dark skin were both associated with an increased prevalence of vitamin D deficiency (olive skin: aPR = 1.14; dark skin: aPR = 1.28), while only dark skin color was associated with hypovitaminosis D (aPR = 1.10). In contrast, protective factors against vitamin D deficiency and hypovitaminosis D included daily sun exposure exceeding one hour (aPR = 0.83–0.91) and vitamin D supplementation (aPR = 0.30–0.55). Anemia, folate deficiency, and vitamin B12 deficiency were significantly associated with a higher prevalence of vitamin D deficiency, hypovitaminosis D, or both. BMI was not significantly associated with vitamin D deficiency; however, women with underweight (aPR = 1.13) and obesity (aPR = 1.12) exhibited a higher prevalence of hypovitaminosis D. Conclusions: Vitamin D deficiency and hypovitaminosis D affect a significant portion of non-pregnant women in Lebanon, with veiling (hijab wearing), limited sun exposure, and lack of supplementation as primary risk factors. Future work should focus on tailoring recommendations for vitamin D supplementation, sun exposure, and food fortification to effectively address the diverse risk factors in the population.