Tissue engineering (TE) demands scaffolds that have the necessary resistance to withstand the mechanical stresses once implanted in our body, as well as excellent biocompatibility. Hydrogels are postulated as interesting materials for this purpose, especially those made from biopolymers. In this study, the microstructure and rheological performance, as well as functional and biological properties of chitosan and collagen hydrogels (CH/CG) crosslinked with different coupling agents, both natural such as d-Fructose (F), genipin (G) and transglutaminase (T) and synthetic, using a combination of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride with N-hydroxysuccinimide (EDC/NHS) will be assessed. FTIR tests were carried out to determine if the proposed crosslinking reactions for each crosslinking agent occurred as expected, obtaining positive results in this aspect. Regarding the characterization of the properties of each system, two main trends were observed, from which it could be established that crosslinking with G and EDC-NHS turned out to be more effective and beneficial than with the other two crosslinking agents, producing significant improvements with respect to the base CH/CG hydrogel. In addition, in vitro tests demonstrated the potential application in TE of these systems, especially for those crosslinked with G, T and EDC-NHS.

The regeneration of soft tissues that connect, support or surround other tissues is of great interest. In this sense, hydrogels have great potential as scaffolds for their regeneration. Among the different raw materials, chitosan stands out for being highly biocompatible, which, together with its biodegradability and structure, makes it a great alternative for the manufacture of hydrogels. Therefore, the aim of this work was to develop and characterize chitosan hydrogels. To this end, the most important parameters of their processing, i.e., agitation time, pH, gelation temperature and concentration of the biopolymer used were rheologically evaluated. The results show that the agitation time does not have a significant influence on hydrogels, whereas a change in pH (from 3.2 to 7) is a key factor for their formation. Furthermore, a low gelation temperature (4 °C) favors the formation of the hydrogel, showing better mechanical properties. Finally, there is a percentage of biopolymer saturation, from which the properties of the hydrogels are not further improved (1.5 wt.%). This work addresses the development of hydrogels with high thermal resistance, which allows their use as scaffolds without damaging their mechanical properties.

The high production rate of conventional plastics and their low degradability result in severe environmental problems, such as plastic accumulation and some other related consequences. One alternative to these materials is the production of oil-free bioplastics, based on wastes from the agro-food industry, which are biodegradable. Not only is rice bran an abundant and non-expensive waste, but it is also attractive due to its high protein and starch content, which can be used as macromolecules for bioplastic production. The objective of this work was to develop rice-bran-based bioplastics by injection moulding. For this purpose, this raw material was mixed with a plasticizer (glycerol), analysing the effect of three mould temperatures (100, 130 and 150 °C) on the mechanical and microstructural properties and water absorption capacity of the final matrices. The obtained results show that rice bran is a suitable raw material for the development of bioplastics whose properties are strongly influenced by the processing conditions. Thus, higher temperatures produce stiffer and more resistant materials (Young’s modulus improves from 12 ± 7 MPa to 23 ± 6 and 33 ± 6 MPa when the temperature increases from 100 to 130 and 150 °C, respectively); however, these materials are highly compact and, consequently, their water absorption capacity diminishes. On the other hand, although lower mould temperatures lead to materials with lower mechanical properties, they exhibit a less compact structure, resulting in enhanced water absorption capacity.